CN108651391B - Device and method for rapidly propagating Dermatophagoides pteronyssinus - Google Patents

Abstract

The invention discloses a device and a method for rapidly propagating Dermatophagoides pteronyssinus, which are characterized by comprising the following steps: the device comprises a storage box (1), a coop (2) and a trap (3), wherein the coop (2) is placed in the storage box (1), and the coop (2) is not in contact with the peripheral side wall of the storage box (1); a plurality of trappers (3) detachably set up around the bottom of chicken coop (2), trappers (3) are transparent hollow cylindric household utensils, just trappers (3) both ends opening, and packing cloth piece (31) in it, cloth piece (31) are in trappers (3) are interior to be folded and are formed many places fold. The collecting device integrates trapping and breeding, has a simple structure, is convenient to disassemble, and can be used for observing breeding dynamics at any time by researchers to realize the collection of the living bodies of the Dermatophagoides pteronyssinus.

Description

Device and method for rapidly propagating Dermatophagoides pteronyssinus

Technical Field

The invention relates to a device integrating trapping and breeding, in particular to a device and a method capable of efficiently trapping and rapidly breeding dermanyssus gallinae.

Background

The Dermatophagoides pteronyssinus is an ectoparasitic arthropod of chickens, is very popular, is fed with blood, easily causes the problems of anemia, emaciation, growth and development retardation and the like of chicks, reduces the laying rate of laying hens, influences the quality of eggs, can cause death of the chickens in severe cases, has severe influence on the development of the poultry industry, and is also a transmission medium of various pathogens. Dermatophagoides pteronyssinus and the diseases transmitted by the same cause economic losses of up to billions of dollars each year to the world chicken industry.

In the process of extracting natural antigen from the body of the Dermatophagoides gallinae to produce the Dermatophagoides gallinae vaccine, a large amount of Dermatophagoides gallinae is needed. Reliable sources of Dermatophagoides gallinae are also needed in the evaluation of the efficacy of drugs and vaccines against Dermatophagoides gallinae. At present, due to the lack of a mature and stable breeding technology of the Dermatophagoides pteronyssinus, when a large amount of Dermatophagoides pteronyssinus is needed, the Dermatophagoides pteronyssinus needs to be collected from a chicken farm, and frequent sampling from the chicken farm consumes a large amount of manpower, material resources and financial resources; and the Dermatophagoides pteronyssinus is mainly highly developed in summer and autumn, a large amount of Dermatophagoides pteronyssinus cannot be collected in a farm in winter and spring, and seasonal circulation of the Dermatophagoides pteronyssinus is an important factor for restricting the production of Dermatophagoides pteronyssinus vaccines and the research of the Dermatophagoides pteronyssinus.

Currently, there are two main ways for the propagation of Dermatophagoides gallinae: host animal breeding and in vitro culture; researchers Lesna I et al used chickens as host animals and bred Dermatophagoides pteronyssinus by directly sucking blood from chickens. Due to the complexity of the rearing device, dermanyssus gallinae easily climbs into the corners of the device, and finally the staff collects dermanyssus gallinae by cleaning the entire rearing apparatus. The method has complicated operation, and easily leaves out the hidden Dermatophagoides pteronyssinus; a large amount of mites are easily lost when excrement is cleaned in the breeding process, and the breeding efficiency is influenced. Therefore, the number of the Dermatophagoides pteronyssinus in each propagation system is greatly changed (1000-10000), and each propagation system cannot successfully propagate the Dermatophagoides pteronyssinus; in addition, in the process of breeding, because the mites are scattered in all corners of the device, the total number and breeding dynamics of the Dermatophagoides pteronyssinus cannot be monitored at any time.

Some foreign researchers use artificial feeding device to feed Dermatophagoides pteronyssinus, the device mainly comprises three parts of glass tube, biological membrane and blood reservoir, and is used for simulating the process of sucking blood of mite on host. Kirkwood and Zeman found that in contrast to artificial films (such as sealing films), the mites of the dermanyssus gallinae took blood through only the skin membrane of the chicks, i.e. the biofilm, and that the nymphs took blood through only the skin membrane of one-day-old chicks. Harrington et al demonstrated that the rate of feeding Dermatophagoides gallinae reached 32.2% after soaking the artificial membrane in the extract of chicken skin, but this treatment increased the complexity of the Dermatophagoides gallinae propagation process. The latest research result shows that the device has high mite mortality rate and very large mite feeding rate difference in the process of feeding Dermatophagoides pteronyssinus, the feeding rate of Dermatophagoides pteronyssinus in some glass tubes is 0%, and the feeding rate in other tubes can reach 50%, which indicates that the device has poor stability. The prepared skin membrane of the young chicken of one day has the defects of influence on the ingestion rate of the Dermatophagoides pteronyssinus and longer time for breeding a large amount of mites. The device has high technical requirement and poor reproducibility, and is not easy to be widely used. In addition, the artificial feeding device cannot simulate the process of continuously sucking blood of the Dermatophagoides pteronyssinus under the natural condition, and the use of the artificial feeding device in the evaluation process of effects such as medicines and vaccines is limited.

The two methods have certain defects, so that a large number of workers still adopt a mode of sampling by a farm to obtain the Dermatophagoides pteronyssinus sample, corrugated paper is used as a mite trapper in literature reports when the Dermatophagoides pteronyssinus sample is collected by the farm, and narrow gaps, folds and dark spaces of the corrugated paper meet the preference of the Dermatophagoides pteronyssinus to a place where the Dermatophagoides pteronyssinus is inhabited; however, since the corrugated paper has a complicated structure and is opaque, a live mite sample can be obtained only by disassembling the corrugated paper with scissors, and the corrugated paper cannot be reused, is very inconvenient to operate, and destroys the habitat of the Dermatophagoides pteronyssinus and adversely affects the reproduction of the Dermatophagoides pteronyssinus. In order to facilitate counting of mites, most researchers put a bag filled with corrugated paper and Dermatophagoides pteronyssinus in an environment at-20 ℃ to freeze the mites, but the method cannot obtain live mite samples.

Based on the limitations of the above methods, it is urgently needed to develop a device integrating trapping and breeding into a whole so as to realize the rapid breeding of the dermanyssus gallinae and facilitate the sampling and collection of researchers.

Disclosure of Invention

In view of the above problems, the present invention aims to provide a device and a method for rapidly propagating Dermatophagoides pteronyssinus, wherein the device integrates trapping and propagation, has a simple structure, is convenient to disassemble, and can be used by researchers to observe propagation dynamics at any time to realize the sampling and collection of Dermatophagoides pteronyssinus living bodies.

In order to achieve the purpose, the invention adopts the following technical scheme: the device for rapidly propagating the Dermatophagoides pteronyssinus is characterized by comprising a storage box (1), a coop (2) and a trap (3), wherein the coop (2) is placed in the storage box (1), and the coop (2) is not in contact with the peripheral side wall of the storage box (1); a plurality of trappers (3) detachably set up around the bottom of chicken coop (2), trappers (3) are transparent hollow cylindric household utensils, just trappers (3) both ends opening, and packing cloth piece (31) in it, cloth piece (31) are in trappers (3) are interior to be folded and are formed many places fold.

The chicken coop is characterized by further comprising a water tray (4), tap water with a small amount of liquid detergent is filled in the water tray (4), the storage box (1) is communicated with the chicken coop (2) and is placed in the water tray (4), and the periphery of the storage box (1) is far away from the edge of the water tray (4).

The artificial infection device (5) comprises a small glass plate (51) and a large glass plate (52) which are different in specification, wherein the small glass plate (51) is placed in the large glass plate (52) filled with water, so that the small glass plate (51) is separated from the large glass plate (52) in a horizontal mode.

Lateral wall of chicken coop (2) sets up silo (21) and basin (22) excrement dish (23) is placed to the bottom of chicken coop (2), with excrement dish (23) correspond chicken coop (2) leave the side seam all around, make excrement dish (23) can follow the side seam level and take out or put into chicken coop (2).

And vaseline is smeared on the peripheral edges of the top of the storage box (1).

The trap (3) is made of hard materials.

Trapper (3) adopt the centrifuging tube, the upper portion of centrifuging tube is uncovered, and the aperture is seted up to the lower part, and the acantho gallinarum follows the both ends of centrifuging tube climb into or climb out, the specification of centrifuging tube is 1ml ~ 100ml, cloth piece (31) of centrifuging tube intussuseption adopt the cloth that has the fold.

The invention also provides a breeding method based on the device for rapidly breeding the Dermatophagoides pteronyssinus, which comprises the following steps:

1) preparing chicks of the right age, and preparing the whole device;

2) placing chicks in the coops (2), and respectively placing sufficient feed and drinking water in a trough (21) and a water tank (22) of the coops (2);

3) carrying out artificial infection of Dermatophagoides gallinae: picking a plurality of Dermatophagoides farinae into a small glass plate (51) of an artificial infection device (5), placing the small glass plate (51) containing the mites on the top of a chicken coop (2), quickly climbing into a trap (3) in the chicken coop (2) to be used as a hiding part, and then taking out the small glass plate (51);

4) the whole set of device is placed in a climatic incubator, and the Dermatophagoides pteronyssinus begins to breed, wherein the temperature in the incubator is set to be 30 ℃, the humidity is set to be 70% relative humidity, the cycle period of illumination is 24 hours, 12 hours of illumination and 12 hours of night are carried out alternately, and excrement is cleaned once every 2-3 days.

By adopting the technical scheme, the invention has the following advantages: according to the artificial breeding system for the Dermatophagoides pteronyssinus, the coop is placed in the storage box, the chicks are placed in the coop to serve as hosts of the Dermatophagoides pteronyssinus, the plurality of traps are fixed on the periphery of the bottom of the coop, the traps are transparent hollow cylindrical vessels, the cloth sheets filled in the traps form a plurality of folds, an environment suitable for the Dermatophagoides pteronyssinus to inhabit is created, and the artificial breeding system for the Dermatophagoides pteronyssinus is formed.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic diagram of the construction of the trap of the present invention;

FIG. 3 is a schematic view of the construction of the artificial infection apparatus of the present invention;

FIG. 4 is a dynamic change curve of Dermatophagoides pteronyssinus number in all traps in six consecutive weeks after the artificial infection with Dermatophagoides pteronyssinus of the present invention;

FIG. 5 is a dynamic change curve of the total egg laying number of Dermatophagoides pteronyssinus in all traps in six consecutive weeks after the artificial infection with Dermatophagoides pteronyssinus.

Detailed Description

The invention is described in detail below with reference to the figures and examples.

As shown in fig. 1 and 2, the invention provides a device for rapidly propagating dermanyssus gallinae, which comprises a storage box 1, a coop 2 and a trap 3, wherein the coop 2 is placed in the storage box 1, the coop 2 is not contacted with the peripheral side wall of the storage box 1, and a chick is placed in the coop 2 and is used as a host animal of the dermanyssus gallinae to provide blood food for the dermanyssus gallinae; a plurality of trappers 3 detachably set up around the bottom of chicken coop 2, and trapper 3 is the cylindric household utensils of transparent cavity, and 3 both ends openings of trapper, fills cloth piece 31 in it, and cloth piece 31 is folding many places folds of formation in trapper 3, builds the habitat that suitable Dermatophagoides pteronyssinus survives, and it is too tight real that pack cloth piece 31 in trapper 3 can not be, is unfavorable for holding a large amount of Dermatophagoides pteronyssinus like this, and easy damage part mite when dismantling moreover.

Further, for conveniently feeding the chick, the excrement and urine that the clearance chick produced sets up silo 21 and basin 22 at the lateral wall of chicken coop 2, places excrement and urine dish 23 in the bottom of chicken coop 2, leaves the side joint around chicken coop 2 that corresponds with excrement and urine dish 23, makes things convenient for the staff to take out excrement and urine dish 23 from the side joint level, for avoiding the acanthopani mite to gather in excrement and urine and pile up the loss that causes the mite, should in time clear up excrement and urine, generally once every 2 ~ 3 days clear up.

Furthermore, in order to prevent the mites from climbing out of the top edge of the storage box 1, vaseline is coated on the periphery of the top of the storage box 1, and the escaped mites are stuck by the vaseline to form a defense line for preventing the mites from escaping.

Further, the device still includes a water tray 4, contains in the water tray 4 and has added the running water that has a small amount of liquid detergent, puts into the water tray 4 with 1 intercommunication chicken coop 2 of storage tank together, and the edge of keeping away from the water tray 4 all around of storage tank 1, and the liquid detergent can reduce the surface tension of running water, even the mite climbs out storage tank 1 and falls into the aquatic, also can't climb on the marginal lateral wall of water tray 4 to form another way of defence line that prevents the mite escape.

Further, in order to prevent the chick from pecking the trap 3 to destroy the inhabiting environment of the Dermanychus urticae, the trap 3 is made of a hard material.

Furthermore, the trap 3 can adopt a centrifugal tube, the upper part of the centrifugal tube is open, the lower part of the centrifugal tube is provided with a small hole, and the Dermatophagoides pteronyssinus is climbed in or climbed out from the two ends of the centrifugal tube, and the specification of the Dermatophagoides pteronyssinus is 1-100 ml; the cloth 31 filled in the centrifugal tube adopts cloth with folds.

Further, as shown in fig. 3, the device further comprises an artificial infection device 5 which comprises a small glass plate 51 and a large glass plate 52 with different specifications, wherein the small glass plate 51 is placed in the large glass plate 52 filled with water, the small glass plate 51 is filled with the acarus gallinae, and the small glass plate 51 is separated from the large glass plate 52 by water, so that the mites can be prevented from escaping in the picking process.

Based on the device for rapidly propagating the Dermatophagoides pteronyssinus, the invention also provides a method for rapidly propagating the Dermatophagoides pteronyssinus, which comprises the following steps:

1) preparing chicks of the right age, and preparing the whole device;

2) placing chicks in the coops 2, and respectively placing sufficient feed and drinking water in the troughs 21 and 22 of the coops 2;

3) carrying out artificial infection of Dermatophagoides gallinae: picking 220 Dermatophagoides farinae into a small glass plate 51 of an artificial infection device 5, placing the small glass plate 51 containing the mites on the top of a coop 2, quickly climbing into a trap 3 arranged in the coop 2, and taking out the small glass plate 51;

4) the whole set of device is placed in a climatic incubator, and the Dermatophagoides pteronyssinus begins to be bred, wherein the temperature in the incubator is set to be 30 ℃, the humidity is set to be 70% relative humidity, the circulation period of illumination is 24 hours, 12-hour illumination and 12-hour night alternate operation are carried out, and in order to avoid the Dermatophagoides pteronyssinus from accumulating on excrement, excrement is cleaned once every 2-3 days.

Use examples

The device for rapidly propagating the Dermatophagoides pteronyssinus is used for propagating the Dermatophagoides pteronyssinus, and the specific contents are as follows:

three sets of devices are used to form three test groups, propagation is carried out according to the method, and the whole test period is 6 weeks; cleaning excrement in the coops 2 every 2-3 days on average, detaching the traps 3 in each set of device every week, taking out the cloth pieces, placing the cloth pieces in a plate, observing under a Zeiss type microscope, recording the number of live mites, dead mites, blood sucking mites and eggs on the cloth pieces and the proportion of mites in each stage by using a counter, and simultaneously recording the number of the Dermatophagoides gallinae mites on vaseline and in the water tray 4.

The experimental results are as follows:

according to the data recorded in the test process, a dynamic change curve of the total number of the dermanyssus gallinae mites in each set of the trap in the whole test period is formed, as shown in fig. 4;

analyzing the dynamic change curve of the total quantity of the Dermatophagoides farinae Bull, wherein the quantity of the mites is not increased after 0-7 days of pest attack, because the lost mites are equal to the newly increased quantity of the bred mites; after 7d, the mites enter a slow breeding stage, and the number of the mites is continuously increased at a certain rate; the breeding rate of the mites is slightly increased at 35-42d, which indicates that the three test groups successfully breed the Dermatophagoides gallinae; at the sixth week, the total number of the Dermatophagoides pteronyssinus of the three test groups is 10981, 10571 and 13743 respectively, the addition and subtraction standard deviation (Mean plus or minus SD) of the average value is 11765 plus or minus 1725, and the Coefficient of Variation (CV) is 14.7 percent, which shows that the device has good repeatability and stability when breeding Dermatophagoides pteronyssinus. After six weeks of reproduction, the number of mites increased from 220 to about 11765 on average, which increased by about 53.5 times. The device and the method of the invention can breed a large amount of Dermatophagoides pteronyssinus successfully in a short time, and the success rate of artificial infection reaches 100 percent.

According to the data recorded in the test process, a dynamic change curve of the total egg laying number of the Dermatophagoides pteronyssinus in the trap in each set of device in the whole test period is formed, and is shown in figure 5;

analyzing the dynamic change curve of the total egg book of the Dermatophagoides farinae, wherein eggs laid by the mites are fewer at 7 th day after the attack, and the number of the eggs at 7-14 th day begins to increase; 21-42 days, the total egg laying number is greatly increased, the speed is improved, the total number of the Dermatophagoides farinae is correspondingly increased, the number of the mites reaches a certain level in the period, most of the mites gather, the mating and the breeding of the mites are facilitated, and the egg laying number is increased; at the sixth week, the total egg production numbers of the three test groups are 3559, 4032 and 4732 respectively, the average plus-minus standard deviation (Mean ± SD) is 4107.7 ± 590, the Coefficient of Variation (CV) is 14.4%, and after six weeks of propagation, the total egg production number increases from the average 59 to the average 4107 from the first week to the sixth week, and increases by about 69.6 times. The result shows that the device and the method have good repeatability and stability and can quickly and stably propagate the Dermatophagoides pteronyssinus.

Recording the number of dead mites and feeding mites every week within 6 weeks, and calculating the survival rate and feeding rate of the mites, wherein the survival rate is live mite number/(live mite number + dead mite number). times.100%; the results of the feeding rate ═ number of ingested mites/number of live mites × 100% are shown in table 1.

TABLE 1 dynamic changes in the survival and feeding rates of Dermatophagoides gallinae

Analysis table 1 shows that in the test lasting six weeks, the survival rate of the mites is relatively stable, the survival rate of the three test groups is changed between 94.87% and 98.64%, and the average value is maintained above 95%; no significant difference was seen between the three groups. With the prolonging of time, the survival rate of the Dermatophagoides farinae is slightly reduced, mainly because the total number of the Dermatophagoides farinae is increased, the death number of the Dermatophagoides farinae is slightly increased due to the limited living space and the service life of the Dermatophagoides farinae; the high survival rate and stability of the Dermatophagoides pteronyssinus in the system indicate that the Dermatophagoides pteronyssinus is very suitable for the environmental conditions provided by the device of the invention and can be bred more stably.

The feeding rate of the mites in the three test groups is changed between 14.93% and 41.42%, the average value of the feeding rate is over 20%, and the Dermatophagoides pteronyssinus is always in a continuous blood sucking state and is relatively stable. The result shows that the feeding rate of the Dermatophagoides pteronyssinus is relatively stable in the whole test period, and the device provided by the invention can ensure that the growth, development and propagation of the mites can be stably carried out.

The change in the proportion of Dermatophagoides gallinae at each developmental stage per week in the test lasting six weeks is shown in Table 2.

TABLE 2 changes in the proportion of Dermatophagoides pteronyssinus at various developmental stages per week

Analysis of Table 2 reveals that the mean proportion of adults varies between 11.10. + -. 2.57 and 18.68. + -. 5.87% throughout the test period; the nymphs comprise first-stage nymphs and second-stage nymphs, and the average ratio of the nymphs varies between 71.46 +/-2.36 and 81.37 +/-2.85 percent; the average proportion of larvae varied between 7.54 ± 3.14 and 13.04 ± 2.30%. Because the larva develops to a nymph of the first stage, blood suction is not needed in the nymph, and 1-2d is needed, the proportion of the nymph in the total colony of the Dermatophagoides gallinae is minimum; for nymphs, the first-stage nymphs need to suck blood, molt and develop to second-stage nymphs within 1-2d, the second-stage nymphs also need to suck blood, molt and develop to adults within 2-3d, and the total development time is 3-5d, so that the nymphs occupy the largest proportion in the community, and most of the average value is 70-80%.

After the test is finished, the numbers of the dermanyssus gallinae adhered to the vaseline and the mites falling into the water tray in the test period are recorded, and the proportion of the dermanyssus gallinae to the total number of the mites is calculated. The results are shown in Table 3 below,

TABLE 3 Vaseline and the number of mites in the water tray and their ratio to the total number of mites

Analysis of table 3 reveals that the amount of sticking to the vaseline was found in each of the 3 test groups: 102. 97 and 115 which account for less than 1 percent of the total number of the Dermatophagoides pteronyssinus and have an average proportion of 0.89 +/-0.05 percent; the number of the mites falling into the water tray is respectively 11, 10 and 15, and the average proportion of the mites in the total number of the Dermatophagoides gallinae is 0.10 +/-0.01%.

From the results, it can be seen that the device of the present invention applies vaseline on the top peripheral edge of the storage tank 1 to intercept most of the escaped dermanyssus gallinae, there are few mites that can cross the vaseline, and the dermanyssus gallinae crossing the vaseline line is intercepted by the water tray 4. The breeding device thoroughly solves the problem that the Dermatophagoides pteronyssinus escapes. The escape rate of the Dermatophagoides pteronyssinus, namely the sum of the proportion of the Vaseline to the total amount of the Dermatophagoides pteronyssinus in the water tray, is about 1%, the caging rate, namely the proportion of the Dermatophagoides pteronyssinus in the trapper, to the total amount reaches about 99%, and the low escape rate shows that the trapper in the device provides a proper inhabiting environment for the Dermatophagoides pteronyssinus and has a good trapping effect.

The present invention has been described with reference to the above embodiments, and the structure, arrangement, and connection of the respective members may be changed. On the basis of the technical scheme of the invention, the improvement or equivalent transformation of the individual components according to the principle of the invention is not excluded from the protection scope of the invention.

Claims (6)

1. The utility model provides a breed chicken skin acarus's device fast which characterized in that: the device comprises a storage box (1), a coop (2) and a trap (3), wherein the coop (2) is placed in the storage box (1), and the coop (2) is not in contact with the peripheral side wall of the storage box (1); the plurality of traps (3) are detachably arranged around the bottom of the coop (2), the traps (3) are transparent hollow cylindrical vessels, the two ends of each trap (3) are opened, cloth sheets (31) are filled in the traps (3), and the cloth sheets (31) are folded in the traps (3) to form a plurality of folds;

the chicken coop is characterized by further comprising a water tray (4), tap water with a small amount of detergent is filled in the water tray (4), the storage box (1) is communicated with the chicken coop (2) and is placed in the water tray (4), and the periphery of the storage box (1) is far away from the edge of the water tray (4); meanwhile, vaseline is smeared on the peripheral edge of the top of the storage box (1).

2. The apparatus for rapidly propagating Dermatophagoides pteronyssinus as claimed in claim 1, wherein: the artificial infection device (5) comprises a small glass plate (51) and a large glass plate (52) which are different in specification, wherein the small glass plate (51) is placed in the large glass plate (52) filled with water, so that the small glass plate (51) is separated from the large glass plate (52) in a horizontal mode.

3. A device for the rapid propagation of dermanyssus gallinae as claimed in claim 1 or 2, wherein: lateral wall of chicken coop (2) sets up silo (21) and basin (22) excrement dish (23) is placed to the bottom of chicken coop (2), with excrement dish (23) correspond chicken coop (2) leave the side seam all around, make excrement dish (23) can follow the side seam level and take out or put into chicken coop (2).

4. The apparatus for rapidly propagating Dermatophagoides pteronyssinus as claimed in claim 1, wherein: the trap (3) is made of hard materials.

5. The apparatus for rapidly propagating Dermatophagoides pteronyssinus as claimed in claim 4, wherein: trapper (3) adopt the centrifuging tube, the upper portion of centrifuging tube is uncovered, and the aperture is seted up to the lower part, and the acantho gallinarum follows the both ends of centrifuging tube climb into or climb out, the specification of centrifuging tube is 1ml ~ 100ml, pack cloth piece (31) in the centrifuging tube and adopt the cloth that has the fold.

6. A breeding method based on the device for rapidly breeding Dermatophagoides gallinae of claim 3, which comprises the following steps:

1) preparing chicks of the right age, and preparing the whole device;

2) placing chicks in the coops (2), and respectively placing sufficient feed and drinking water in a trough (21) and a water tank (22) of the coops (2);

3) carrying out artificial infection of Dermatophagoides gallinae: picking a plurality of Dermatophagoides farinae into a small glass plate (51) of an artificial infection device (5), placing the small glass plate (51) containing the mites on the top of a coop (2), quickly climbing into a trap (3) in the coop (2) to be used as a hiding part, and taking out the small glass plate (51);

4) the whole set of device is placed in a climatic incubator, and the Dermatophagoides pteronyssinus begins to breed, wherein the temperature in the incubator is set to be 30 ℃, the humidity is set to be 70% relative humidity, the cycle period of illumination is 24 hours, 12 hours of illumination and 12 hours of night are carried out alternately, and excrement is cleaned once every 2-3 days.

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