CN108620144A - A kind of micro-fluid chip being used in WB experiments - Google Patents
A kind of micro-fluid chip being used in WB experiments Download PDFInfo
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- CN108620144A CN108620144A CN201810751117.1A CN201810751117A CN108620144A CN 108620144 A CN108620144 A CN 108620144A CN 201810751117 A CN201810751117 A CN 201810751117A CN 108620144 A CN108620144 A CN 108620144A
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- runner plate
- flow path
- micro
- experiment
- path groove
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
- B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses the micro-fluid chips in a kind of experiment for WB, micro-fluid chip includes support plate successively from the bottom to top, gasket seal, gel film and runner plate, on the support plate and runner plate multiple locating slots are correspondingly provided at side end position, it is inserted in the locating slot and is embedded with positioning pin, the runner plate is equipped with and inserts embedding locating slot for positioning pin, prolong its length direction array and be equipped with A items longitudinal direction flow path groove in the lower surface of the runner plate, one end of the longitudinal direction flow path groove is equipped with the sample holes through runner plate, the other end of the longitudinal direction flow path groove is equipped with through the stock layout hole of runner plate；It can effectively achieve the effect that save antibody, and the Binding experiment for needing to be carried out at the same time Multiple Antibodies according to experiment, improve working efficiency.
Description
Technical field
The present invention relates to the micro-fluid chips in Bioexperiment apparatus field more particularly to a kind of experiment for WB.
Background technology
WESTERN BLOT （WB）Experiment is common a kind of reality in molecular biology, biochemistry and immunogenetics
Proved recipe method.Its basic principle is carried out to the processed cell of gel electrophoresis or biological tissue samples by specific antibody
Color, the position by analyzing coloring obtain specific protein expression in the cell or tissue analyzed with color depth
Information.
Gel electrophoresis mainly is carried out to protein, the Binding experiment of antibody is then carried out on electrophoretic band, usually
The combination of antibody needs gel film to place in a reservoir, antibody is then injected in container, fully shaking container makes antibody
It needs to clean gel film and container after combining primary antibody with the protein binding of gel, to reduce pollution, and meanwhile it is clear
Antibody after washing can give over to experiment next time and be continuing with.
In order to reduce the dosage of antibody, therefore the demand of the WB chips based on microfluid is increasingly strong.
Invention content
The object of the present invention is to provide a kind of microfluid cores for WB experiments, the advantage is that, can effectively reach section
Save the effect of antibody, and the Binding experiment for needing to be carried out at the same time Multiple Antibodies according to experiment.
The present invention above-mentioned technical purpose technical scheme is that:It is a kind of to be used in WB experiments
Micro-fluid chip, micro-fluid chip include support plate, gasket seal, gel film and runner plate, the support plate successively from the bottom to top
With multiple locating slots are correspondingly provided on runner plate at the side end position, inserted in the locating slot and be embedded with positioning pin, it is described
Runner plate, which is equipped with, inserts embedding locating slot for positioning pin, and the lower surface of the runner plate is prolonged its length direction array and indulged equipped with A items
To flow path groove, one end of the longitudinal direction flow path groove is equipped with the sample holes through runner plate, the other end of the longitudinal direction flow path groove
It is equipped with through the stock layout hole of runner plate.
The present invention is further arranged to：Array is equipped with N*M grid experimental port on the runner plate, in the experiment square hole
It is equipped with the negative tube being connect with negative pressure pump.
The present invention is further arranged to：The runner plate, which is gone into battle, shows B group each runner groups, and every group of each runner group is by 4 longitudinal streams
Road slot forms, and one end of flow path groove is equipped with sample holes longitudinally in each, and the other end is equipped with stock layout hole.
The present invention is further arranged to：The gel film is equipped with A transverse flow channels slot, and the runner plate is equipped with C items
One end of the longitudinal flow path groove vertical with transverse flow channels slot, the longitudinal direction flow path groove is equipped with sample holes, the longitudinal direction flow path groove
The other end is equipped with stock layout hole.
The present invention is further arranged to：The gasket seal is density pearl cotton plate.
The present invention is further arranged to：It is located on the gel film at the side of the longitudinal flow path groove of outermost two and is equipped with
Label space.
In conclusion the invention has the advantages that：
1, it may be implemented in gel film surface shape by the compression to runner plate since runner plate bottom is equipped with longitudinal flow path groove
At same longitudinal flow path groove, the sample introduction of antibody is realized by sample holes later so that antibody is stored in longitudinal stream of gel film
With the protein binding of gel film in road slot, there is A items longitudinal direction flow path groove, then the Binding experiment of A kind different antibodies may be implemented, effectively
The wasting phenomenon of antibody is reduced, and kinds of experiments can be carried out at the same time, effectively improves working efficiency；
2, N*M grid experimental port can be arranged in runner plate, can be carried out at the same time the experiment of N*M kind different antibodies, such grid
Experimental port, can in a manner of directly using drop reagent sample introduction, and promote by negative pressure pump and negative tube the combination of antibody, more
It is time saving and energy saving, embody the design of this micro-fluid chip, improve work efficiency simultaneously；
3, B group each runner groups are set on the runner plate in this micro-fluid chip, every group of each runner group is made of 4 longitudinal flow path grooves, this
Kind design scheme has prodigious convenience for doing parallel laboratory test or various samples comparative experiments, further embodies this miniflow
The design and functionality of body chip；
4, this micro-fluid chip can also use longitudinal flow path groove to add the design of transverse flow channels slot, reach longitudinal and lateral point
Analysis；
5, density pearl panel have moisture-proof water proof, shockproof, sound insulation, heat preservation, plasticity is good, toughness is strong, reconstituted circularly, environmental protection,
Many advantages, such as crashworthiness power is strong also has good chemical resistance；
6, setting flag space on runner plate, can be marked, and be referred to the position of marker, convenient for the data point in later stage
Analysis.
Description of the drawings
Fig. 1 is the overall structure diagram of this micro-fluid chip in embodiment 1；
Fig. 2 is the schematic diagram for having on gel film in embodiment 1 longitudinal flow path groove and label space；
Fig. 3 is the schematic diagram of runner plate in embodiment 2；
Fig. 4 is the schematic diagram of runner plate in embodiment 3；
Fig. 5 is the schematic diagram of transverse flow channels slot and longitudinal flow path groove in embodiment 4；
Fig. 6 is the schematic diagram that transverse flow channels slot is combined with sub-thread longitudinal direction flow path groove in embodiment 4；
Fig. 7 is the schematic diagram of multiply longitudinal direction flow path groove and transverse flow channels slot in embodiment 4.
In figure：1, support plate；11, positioning pin；2, gasket seal；3, gel film；4, runner plate；41, locating slot；42, sample introduction
Hole；43, stock layout hole；44, negative tube；5, longitudinal flow path groove；6, grid experimental port；7, transverse flow channels slot；8, label space.
Specific implementation mode
Below in conjunction with attached drawing, invention is further described in detail.
Embodiment 1：It is a kind of for WB experiment in micro-fluid chip, as illustrated in fig. 1 and 2, this micro-fluid chip by down toward
On include successively support plate 1, gasket seal 2, gel film 3 and runner plate 4, it is equal at side end position on support plate 1 and runner plate 4
Multiple locating slots 41 are correspondingly provided with, are connected and fixed by the realization of positioning pin 11 in locating slot 41, gasket seal 2 is density pearl
Cotton plate, runner plate 4 are equipped with and insert embedding locating slot 41 for positioning pin 11, at the same time, prolong its length in the lower surface of runner plate 4
Direction array is equipped with A items longitudinal direction flow path groove 5, and one end of longitudinal flow path groove 5 is equipped with the sample holes 42 through runner plate 4, longitudinal
The other end of flow path groove 5 is equipped with through the stock layout hole 43 of runner plate 4.
As illustrated in fig. 1 and 2, according to from bottom to up be support plate 1, positioning pin 11, gasket seal 2, gel film 3 and runner plate 4
Sequence installed successively so that positioning pin 11 respectively insert embedded in support plate 1 locating slot 41 in, later successively by gasket seal
2, gel film 3 is placed on support plate 1, finally applies certain pressure to runner plate 4 so that the locating slot 41 of runner plate 4 also with
The connection installation of positioning pin 11 realizes the integrated connection installation of this micro-fluid chip, during being fixedly mounted herein, to gel at this time
Piece 3 produces certain pressure, and under the effect of this pressure, longitudinal flow path groove 5 of 4 bottom surface of runner plate is contacted with gel film 3, and is made
The generation of 3 surface of gel film and longitudinal flow path groove 5 completely same on runner plate 4 are obtained, the antibody of WB experiments can be carried out at this time
Multiple Antibodies are passed through every longitudinal flow path groove 5 by Binding experiment again by the input mode of sample needle or pipe fitting respectively
Sample holes 42 realize the sample introduction of antibody so that antibody flows into anti-with condensation sheet protein binding in longitudinal flow path groove 5 of gel film 3
It answers；At this point, a kind of antibody or the Multiple Antibodies Binding experiment with gel film 3 simultaneously can be thus achieved, and do not hamper from each other
Hinder influence, the working time is greatly saved, improves work efficiency, finally, the antibody for completing experiment is discharged by stock layout hole 43,
It will not cause the waste of antibody.
In the present embodiment, as shown in Fig. 2, being located on gel film 3 equal at the side of two longitudinal direction flow path grooves 5 of outermost
Equipped with label space 8, during the experiment, it can be marked, test so subsequent on the label space 8 of gel film 3
Exposure may be used to determine experiment Conductance calculation or comparison, more scientific more acurrate.
In the present embodiment, support plate 1 and runner plate 4 are using materials such as PMMA, PC, COC, glass, and are transparent, with
Just clear observation experiment combination situation in experimentation, CNC processing may be used in the manufacture of support plate 1 and runner plate 4, injection molding adds
The technique runner plate 4 of work either photoetching corrosion forms longitudinal runner after injection molding, CNC processing or the techniques such as photoetching corrosion
Slot 5.
Embodiment 2：A kind of micro-fluid chip being used in WB experiments, difference from example 1 is that, such as Fig. 3 institutes
Showing, array is equipped with N*M grid experimental port 6 on runner plate 4 at this time, directly drip can be used to realize antibody sample introduction at this time,
Multiple Antibodies carry out antibody binding test in the N*M grid experimental port 6 respectively, are equipped in grid experimental port 6 and negative pressure pump
（It is not represented in figure）The negative tube 44 of connection by the work of negative pressure pump, adds in the process into a grid experimental port 6
The mode for entering negative pressure promotes the combination of antibody, to improve working efficiency, while promoting the combination of antibody.
Embodiment 3：A kind of micro-fluid chip being used in WB experiments, difference from example 1 is that, such as Fig. 4 institutes
Show, runner plate 4, which is gone into battle, shows B group each runner groups, every group of each runner group is made of 4 longitudinal flow path grooves 5, longitudinally in each flow path groove 5
One end is equipped with sample holes 42, and the other end is equipped with stock layout hole 43, i.e. antibody in sample introduction a, b, c, d tetra- in every group of each runner group, this
Kind design scheme has prodigious convenience for doing parallel laboratory test or various samples comparative experiments.
Embodiment 4：A kind of micro-fluid chip being used in WB experiments, difference from example 1 is that, such as Figures 5 and 6
It is shown, the transverse flow channels slot 7 of A sub-thread is equipped on gel film 3, runner plate 4 is equipped with C items work vertical with transverse flow channels slot 7
Longitudinal flow path groove 5, as shown in fig. 7, go into battle in runner plate 4 and show B group each runner groups, every group of each runner group is by 4 longitudinal directions
Flow path groove 5 forms, and one end of longitudinal flow path groove 5 is equipped with sample holes 42, and the other end of longitudinal flow path groove 5 is equipped with stock layout hole 43, coagulates
A plurality of transverse flow channels slot 7 is provided on film 3 in advance, during realizing the integral installation of this micro-fluid chip, is made in pressure
Under, longitudinal flow path groove 5 on runner plate 4 acts on gel film 3, that is, forms the longitudinal runner communicated with transverse flow channels slot 7
Slot 5, to realize the diversified Binding experiment of antibody.
This specific embodiment is only explanation of the invention, is not limitation of the present invention, people in the art
Member can as needed make the present embodiment the modification of not creative contribution after reading this specification, but as long as at this
It is all protected by Patent Law in the right of invention.
Claims (6)
1. the micro-fluid chip in a kind of experiment for WB, it is characterised in that：Micro-fluid chip includes support plate successively from the bottom to top
(1), gasket seal (2), gel film (3) and runner plate (4), on the support plate (1) and runner plate (4) at side end position
Multiple locating slots (41) are correspondingly provided with, is inserted in the locating slot (41) and is embedded with positioning pin (11), set on the runner plate (4)
Have and insert embedding locating slot (41) for positioning pin (11), the lower surface of the runner plate (4) is prolonged its length direction array and indulged equipped with A items
To flow path groove (5), one end of the longitudinal direction flow path groove (5) is equipped with the sample holes (42) through runner plate (4), longitudinal stream
The other end of road slot (5) is equipped with through the stock layout hole (43) of runner plate (4).
2. the micro-fluid chip in a kind of experiment for WB according to claim 1, it is characterised in that：The runner plate
(4) array is equipped with N*M grid experimental port (6), the experiment square hole on（6）Inside it is equipped with the negative tube being connect with negative pressure pump
(44)。
3. the micro-fluid chip in a kind of experiment for WB according to claim 1, it is characterised in that：The runner plate
(4) it goes into battle and shows B group each runner groups, every group of each runner group is made of 4 longitudinal flow path grooves (5), longitudinally in each one end of flow path groove (5)
Sample holes (42) are equipped with, the other end is equipped with stock layout hole (43).
4. the micro-fluid chip in a kind of experiment for WB according to claim 1, it is characterised in that：The gel film
(3) it is equipped with A transverse flow channels slot (7), the runner plate (4) is equipped with the C items longitudinal runner vertical with transverse flow channels slot (7)
One end of slot (5), the longitudinal direction flow path groove (5) is equipped with sample holes (42), and the other end of the longitudinal direction flow path groove (5) is equipped with stock layout
Hole (43).
5. the micro-fluid chip in a kind of experiment for WB according to claim 1, it is characterised in that：The gasket seal
(2) it is density pearl cotton plate.
6. the micro-fluid chip in a kind of experiment for WB according to claim 1 and 3, it is characterised in that：The gel
It is located on piece (3) at the side of the longitudinal flow path groove (5) of outermost two and is equipped with label space (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810751117.1A CN108620144A (en) | 2018-07-10 | 2018-07-10 | A kind of micro-fluid chip being used in WB experiments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810751117.1A CN108620144A (en) | 2018-07-10 | 2018-07-10 | A kind of micro-fluid chip being used in WB experiments |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108620144A true CN108620144A (en) | 2018-10-09 |
Family
ID=63688784
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810751117.1A Pending CN108620144A (en) | 2018-07-10 | 2018-07-10 | A kind of micro-fluid chip being used in WB experiments |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1423131A (en) * | 2002-12-30 | 2003-06-11 | 陕西超英生物医学研究开发有限公司 | Composite chip for detecting autoimmune antibody of diabetes mellitus, and preparation and detection method thereof |
| WO2004072642A1 (en) * | 2003-02-17 | 2004-08-26 | Zhanhui Wang | A device for application of micro-sample and reaction of a biochip and its method |
| US20060246416A1 (en) * | 2003-07-31 | 2006-11-02 | Hirofumi Tani | On-chip bioassay method and kit |
| CN101629953A (en) * | 2009-08-07 | 2010-01-20 | 中国海洋大学 | On-site detection immuno-chip and preparation method thereof and application |
| CN102087292A (en) * | 2009-12-02 | 2011-06-08 | 国家纳米科学中心 | Microfluidic immune imprinting chip and preparation method and application thereof |
| CN104360060A (en) * | 2014-11-14 | 2015-02-18 | 国家纳米科学中心 | Detection method for specific antibodies IgM of mycoplasma pneumonia and influenza viruses based on micro-fluidic chip |
| CN104437687A (en) * | 2014-11-04 | 2015-03-25 | 南开大学 | Three-dimensional array type microfluid sample introduction/cleaning chip |
| CN105063168A (en) * | 2015-07-17 | 2015-11-18 | 上海交通大学 | Method and device for detecting free epithelial cell organ sources in blood |
| CN208810099U (en) * | 2018-07-10 | 2019-05-03 | 南京宝沃生物科技有限公司 | A kind of micro-fluid chip in WB experiment |
-
2018
- 2018-07-10 CN CN201810751117.1A patent/CN108620144A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1423131A (en) * | 2002-12-30 | 2003-06-11 | 陕西超英生物医学研究开发有限公司 | Composite chip for detecting autoimmune antibody of diabetes mellitus, and preparation and detection method thereof |
| WO2004072642A1 (en) * | 2003-02-17 | 2004-08-26 | Zhanhui Wang | A device for application of micro-sample and reaction of a biochip and its method |
| US20060246416A1 (en) * | 2003-07-31 | 2006-11-02 | Hirofumi Tani | On-chip bioassay method and kit |
| CN101629953A (en) * | 2009-08-07 | 2010-01-20 | 中国海洋大学 | On-site detection immuno-chip and preparation method thereof and application |
| CN102087292A (en) * | 2009-12-02 | 2011-06-08 | 国家纳米科学中心 | Microfluidic immune imprinting chip and preparation method and application thereof |
| CN104437687A (en) * | 2014-11-04 | 2015-03-25 | 南开大学 | Three-dimensional array type microfluid sample introduction/cleaning chip |
| CN104360060A (en) * | 2014-11-14 | 2015-02-18 | 国家纳米科学中心 | Detection method for specific antibodies IgM of mycoplasma pneumonia and influenza viruses based on micro-fluidic chip |
| CN105063168A (en) * | 2015-07-17 | 2015-11-18 | 上海交通大学 | Method and device for detecting free epithelial cell organ sources in blood |
| CN208810099U (en) * | 2018-07-10 | 2019-05-03 | 南京宝沃生物科技有限公司 | A kind of micro-fluid chip in WB experiment |
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