CN108503050B - Method for preparing boiler water regulator by microbial fermentation method - Google Patents
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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Abstract
The invention discloses a method for preparing a boiler water regulator by a microbial fermentation method, which comprises the following raw materials in parts by weight: 5-10 parts of sodium tannate, 3-5 parts of glucose, 5-12 parts of seaweed, 3 parts of ethylene glycol derivatives, 3-5 parts of cotton straws, 2 parts of starch, 1 part of deoxidant and the balance of water, and the boiler water regulator is prepared by a microbial fermentation method, so that the requirements of boiler water regulation on raw materials are reduced, and the effect of the boiler water regulator is remarkably improved by the flocculation effect of thermal denaturation of microbial protein; the regulator does not contain harmful and toxic substances such as phosphate, hydrazine compounds and the like, controls the oxygen corrosion and the condensate water corrosion of the boiler, can greatly improve the boiler water concentration multiple, reduces the boiler pollution discharge rate, saves water resources and heat energy, and realizes the aims of energy conservation and emission reduction.
Description
Technical Field
The invention relates to a method for preparing a boiler water regulator by a microbial fermentation method, in particular to a novel environment-friendly boiler water regulator which is based on resource utilization of seaweed, straw and the like and does not contain sulfur and toxic deoxidant, belonging to the field of biochemistry.
Background
In recent years, most of boiler water and industrial circulating water (such as circulating cooling water of nitrogen fertilizer plants, oxygen plants and pharmaceutical factories) are treated by phosphate formulations, commonly used are orthophosphate, pyrophosphate, sodium tripolyphosphate, sodium hexametaphosphate and the like, and the water treatment agent has good corrosion and scale inhibition effects and wide application range, but has the following defects in use: (ii) PO4 3-Has no threshold effect on scale inhibition, so that the method has no scale inhibition effect and only can generate Ca10(OH)2(PO4)6The water granulated slag is formed according to the stoichiometric amount, the discharge capacity is large, and the boiler water cannot run under high concentration times. In order to discharge phosphate, a boiler water blowdown bottom valve needs to be discharged every day, so that frequent leakage is easily caused; ② PO4 3-The scale has no anti-corrosion function and is a scale forming group, and passivation protection cannot be provided for the boiler, so that the scale formation and the sediment generation cannot be effectively inhibited; ③ PO4 3-The buffer capacity for the pH of boiler water is limited and "temporary disappearance" of phosphate is likely to occur when boiler load changes, resulting in excessive phosphate addition. Calcium, magnesium and other salts generated with trisodium phosphate boiler water regulator are easy to enter the steam system due to entrainment, so that scaling and pipeline corrosion of a steam compressor of the steam system are caused, and the phenomenon is represented by Na in the steam system+、SiO2The content of the same is higher. Fifthly, the phosphate is entrained into the steam and deposited on the blades of the steam press, which affects the long-term safe and stable operation of the steam press. In addition, as a large amount of phosphorus is discharged into a water area, eutrophication of water occurs, red tide occurs, bacteria and algae in the water are propagated, and further oxygen deficiency in the water can be caused, so that aquatic organisms die, and not only is aquatic products damaged, but also the ecological environment is damaged. Most of the boiler water treatment agents in the prior art still contain a certain amount of phosphate series, for example, patent 201510405747.X (published Japanese 2017.01.18) discloses a boiler water treatment agent, the raw materials of which contain 5-10 parts of phosphate seriesSodium sulfite; patent 201610376456.7 (published Japanese 2016.08.03) discloses a water treatment agent for biomass boilers, which comprises raw materials of 15-19 parts of catalytic potassium sulfite, 8-13 parts of hydroxyethylidene diphosphonic acid and 5-8 parts of trisodium phosphate; patent 201610636723.X (published japanese 2017.01.04) discloses a composite boiler water treatment agent composition, which comprises 2-15% of polyphosphate in raw materials.
This problem has been generally regarded by various countries, and some research institutes and researchers have developed a phosphorus-free low-sodium boiler water treatment agent, for example, patent 201710128659.9 (published japanese 2017.06.13) discloses a phosphorus-free low-sodium boiler water treatment agent, which comprises the following raw material components: polyalkylacrylates, polymaleates, polyepoxysuccinates, benzo compounds, sodium salts, and the like; patent 201710127999.X (published Japanese 2017.06.09) discloses a phosphorus-and sodium-free boiler water treatment agent, which comprises raw materials of polymaleic anhydride, polyepoxysuccinic glycoside, organic amines, benzo compounds and the like. Although the phosphorus-free boiler water treatment agent is analyzed, although phosphorus is not contained, benzo compounds, organic ammonia and other substances with strong toxicity in the raw materials have suspected carcinogenic effect, are easy to splash onto eyes, skin or clothes of a person during operation, are easy to be inhaled by the human body, and influence the health of operators; the hydrazine has strong volatility, is inflammable and explosive, and when the volume fraction of hydrazine vapor in the air reaches 4.7 percent. Deflagration occurs when meeting fire, which brings trouble to transportation, storage and use, and countries such as Europe, America, Japan and the like have abandoned the chemical substances.
Therefore, the raw materials of the boiler water regulator are mainly chemical products at present, the preparation process of the boiler water regulator mainly adopts the processes of proportioning and mixing, and other preparation processes are not found at present, particularly the report of preparing the boiler water regulator by using a microbial fermentation method is not found.
In order to replace the traditional phosphate and sodium hydroxide treatment method and the use of hydrazine compounds, the boiler is operated more efficiently, environmentally and safely, and the development of a novel green, efficient and safe boiler water treatment agent becomes necessary.
Disclosure of Invention
In order to overcome the defects of the prior art and respond to the environmental protection and energy saving policies of energy conservation, emission reduction and consumption reduction advocated at home and abroad, the application provides the method for preparing the boiler water regulator by using the microbial fermentation method, the method utilizes the microbial fermentation method to prepare the boiler water regulator, not only reduces the requirements of boiler water regulation on raw materials, but also obviously improves the action effect of the boiler water regulator by utilizing the flocculation effect of thermal denaturation of microbial protein; the regulator does not contain harmful and toxic substances such as phosphate, hydrazine compounds and the like, controls the oxygen corrosion and the condensate water corrosion of the boiler, can greatly improve the boiler water concentration multiple, reduces the boiler pollution discharge rate, saves water resources and heat energy, realizes the aims of energy conservation and emission reduction, and further improves the economic benefit; the content of silicon dioxide in the steam can be effectively reduced, the silicon scale can be greatly reduced, and the problem of silicon scale generation on a boiler and a steam turbine can be even avoided.
The invention realizes the technical effects through the following technical scheme:
a method for preparing a boiler water regulator by a microbial fermentation method is characterized by comprising the following raw materials in parts by weight: 5-10 parts of sodium tannate, 3-5 parts of glucose, 5-12 parts of seaweed, 3 parts of ethylene glycol derivative, 3-5 parts of cotton straw, 2 parts of starch, 1 part of oxygen scavenger and the balance of water, wherein the total weight is 100 parts.
Preferably, the method for preparing the boiler water regulator by the microbial fermentation method is characterized in that the boiler water regulator contains the following raw materials in parts by weight: 6.8 parts of sodium tannate, 4 parts of glucose, 9.2 parts of seaweed, 3 parts of ethylene glycol derivative, 4 parts of cotton straw, 2 parts of starch, 1 part of deoxidant and 70 parts of purified water.
The seaweed is one or mixture of herba Zosterae Marinae and Enteromorpha.
The oxygen scavenger is a mixture of acetaldoxime and diethylhydroxylamine, and the mass ratio of the acetaldoxime to the diethylhydroxylamine is 7: 3.
The application provides a method for preparing a boiler water regulator by a microbial fermentation method, which is characterized in that the microorganisms comprise aspergillus niger, saccharomycetes and bacillus subtilis, and the inoculation ratio of the aspergillus niger to the saccharomycetes to the bacillus subtilis is 1:1: 1.
The yeast can be one or more of Candida utilis, cerevisiae fermentum, Saccharomyces cerevisiae, and molasses yeast;
preferably, the best strain of the yeast is a mixed strain of candida utilis and molasses yeast, and the dosage ratio of the mixed strain to the molasses yeast is 1: 0.75.
The preparation method of the Aspergillus niger seed liquid comprises the following steps: culturing for 24h at 35-40 deg.C in potato culture medium under aseptic condition to obtain Aspergillus niger seed liquid with effective viable count not less than 109cfu/mL。
The preparation method of the yeast seed liquid comprises the following steps: under the aseptic operation condition, two rings of strains are picked from the inclined plane of the yeast YDP by using an inoculating ring, inoculated into a 500mL triangular flask containing 300mLPDA liquid culture medium, subjected to shaking culture at the temperature of between 28 and 32 ℃ and at the speed of 140r/min for 24 hours, and then inoculated into a seed tank for expanded culture according to the process to prepare the yeast seed liquid, wherein the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL。
The preparation method of the bacillus subtilis seed solution comprises the following steps: under the aseptic condition, LB culture medium is adopted to culture for 24 hours at the temperature of 28-32 ℃ to prepare liquid bacillus seed liquid, and the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL。
The invention provides a method for preparing a boiler water regulator by a microbial fermentation method, which is characterized in that the boiler water regulator is prepared by the following processes:
(1) pretreatment of raw materials: pulverizing seaweed and cotton straw, and sieving with 100 mesh sieve;
(2) preparing materials: accurately mixing sodium tannate, glucose, seaweed, glycol derivatives, cotton straws, starch and a deoxidant in proportion;
(4) mixing: adding the mixed raw materials into purified water through a dosing device, and uniformly mixing;
(5) and (3) microbial fermentation: inoculating the activated aspergillus niger, saccharomycetes and bacillus subtilis to the uniformly mixed material in the step (4) according to the proportion of 3-5%, and performing ventilation fermentation culture for 24-48H at the temperature of 28-35 ℃;
(6) and (3) filtering: filtering the fermented material to remove insoluble substances;
(7) and (4) subpackaging, namely accurately subpackaging the filtered materials to prepare the boiler water treatment agent.
The application provides a boiler water treatment agent prepared by a microbial fermentation method, raw materials comprise sodium tannate, glucose, seaweed, glycol derivatives, cotton straws, starch, an oxygen scavenger and the like, and the boiler water treatment agent is prepared by a composite microbial fermentation process. The raw materials are as follows: the sodium tannate functions to form a tannate preservative protective film and to adsorb oxygen. The tannate is the best boiler water additive for preventing corrosion at present, and can adsorb oxygen in water to form a tannate protective film (namely a magnetite protective film), so that the corrosion problem is avoided; the glucose is used for promoting the growth and breeding of microorganisms; the seaweed has the effects that the complexing action of active substances such as polysaccharide, polypeptide and the like in the seaweed on metal ions forms a protective film on the surface of metal through the microbial fermentation effect, so that the corrosion problem is avoided, the generation of scale can be prevented through the adsorption effect of the seaweed, and the foaming is inhibited; the glycol derivative has the effects of inhibiting the formation of foam, ensuring pure steam, and improving the concentration multiple of boiler water so as to reduce the pollution discharge rate of boiler water, save water and reduce the heat loss of pollution discharge; the cotton straw is degraded into small molecular substances through the fermentation of aspergillus niger, bacillus subtilis and the like, and then plays a role in loosening and decomposing boiler scale; the starch has the functions of adjusting the sludge, improving the adsorption capacity of the sludge on water scale and reducing the pollution discharge rate of the boiler; the purpose of the deoxidant is to quickly consume dissolved oxygen in water, and the surface oxygen has oxidation effect on metals such as iron and the like; the flocculation effect of the microbial mycoprotein generated in the fermentation process due to thermal denaturation can obviously improve the effect of the boiler water regulator. The components complement each other to cooperatively form the effects of preventing scale, corrosion and scale, avoiding steam and water co-transpiration and the like.
Compared with the prior art, the method has the following beneficial effects:
(1) according to the boiler water regulator prepared by the microbial fermentation method, microbial fermentation treatment is carried out on raw materials such as cotton straws, seaweed and starch by utilizing the fermentation effect of aspergillus niger, saccharomycetes and bacillus subtilis, the effect of the raw materials in the boiler water regulator is improved, the requirement of a boiler water treatment agent on the raw materials is lowered, and excessive application of toxic and harmful chemicals is avoided. Meanwhile, the effect of the boiler water treatment agent is obviously improved through the synergistic interaction of the microorganisms;
(2) the water treatment agent for the boiler is creatively added with the components such as seaweed, cotton straws, starch and the like, and is used with organic and inorganic compounds such as sodium tannate, glucose, glycol derivatives, deoxidant and the like through the fermentation effect of microorganisms, so that the scale prevention, corrosion prevention and scale removal of the water treatment agent for the boiler are remarkably improved through the synergistic effect among the components, and the effects of steam-water co-rising and the like are avoided; but also can remove old scales existing in the boiler to form flowable sludge which is discharged out of the boiler body through periodic pollution discharge; the heat efficiency of the boiler is improved, and the extra fuel consumption of the boiler is reduced;
(2) the boiler water treatment agent has stronger codeposition effect, improves the shock resistance of boiler water, enhances the adaptability of a boiler to feed water, and further improves the metal corrosion resistance of the boiler water;
(3) the problem of phosphate hiding which is very easy to occur to a boiler under the working condition of phosphate water and the risk of alkaline corrosion or acidic phosphate corrosion caused by the phosphate hiding are eliminated, so that the leakage of a furnace tube is reduced;
(4) the requirements of the boiler on the quality of feed water are relaxed, the requirements of the working condition of water in the boiler on the aspects of technical management, investment of water treatment facilities and the like are reduced, the treatment level in the boiler with medium and high parameters is aligned with a large-capacity and high-parameter unit, the service life of the boiler is prolonged, the maintenance cost is reduced, and the output level of the system is maintained;
(5) the technical indexes of boiler water and the economic indexes such as boiler pollution discharge rate are closely and organically connected, so that the economic purpose of technical index management is highlighted, the management is improved, and the production economy is improved.
Detailed Description
The present invention will be further described with reference to the following embodiments.
Example 1
A method for preparing a boiler water regulator by a microbial fermentation method comprises the following specific process steps:
(1) pretreatment of raw materials: pulverizing seaweed and cotton straw, and sieving with 100 mesh sieve; the seaweed is a mixture of kelp and enteromorpha in a mass ratio of 1: 1;
(2) preparing materials: accurately mixing sodium tannate, glucose, seaweed, glycol derivatives, cotton straws, starch and a deoxidant according to the proportion of 6.8 parts of sodium tannate, 4 parts of glucose, 9.2 parts of seaweed, 3 parts of glycol derivatives, 4 parts of cotton straws, 2 parts of starch, 1 part of deoxidant and 70 parts of purified water;
(4) mixing: adding the mixed raw materials into purified water through a dosing device, and uniformly mixing;
(5) activating strains: aspergillus niger, yeast and Bacillus subtilis were activated as follows:
the preparation method of the Aspergillus niger seed liquid comprises the following steps: culturing for 24h at 35-40 deg.C in potato culture medium under aseptic condition to obtain Aspergillus niger seed liquid with effective viable count not less than 109cfu/mL；
The preparation method of the yeast seed liquid comprises the following steps: under the aseptic operation condition, two rings of strains are picked from the inclined plane of the yeast YDP by using an inoculating ring, inoculated into a 500mL triangular flask containing 300mLPDA liquid culture medium, subjected to shaking culture at the temperature of between 28 and 32 ℃ and at the speed of 140r/min for 24 hours, and then inoculated into a seed tank for expanded culture according to the process to prepare the yeast seed liquid, wherein the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL; the yeast is a mixed strain of candida utilis and molasses yeast, and the using amount ratio of the yeast is 1: 0.75;
the preparation method of the bacillus subtilis seed solution comprises the following steps: under the aseptic condition, LB culture medium is adopted to culture for 24 hours at the temperature of 28-32 ℃ to prepare liquid bacillus seed liquid, and the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL；
(6) And (3) microbial fermentation: inoculating the activated aspergillus niger, saccharomycetes and bacillus subtilis to the uniformly mixed material in the step (4) according to the proportion of 4%, and performing ventilation fermentation culture for 24-36H at the temperature of 28-35 ℃;
(7) and (3) filtering: filtering the fermented material to remove insoluble substances;
(8) and (4) subpackaging, namely accurately subpackaging the filtered materials to prepare the boiler water treatment agent.
Example 2
A method for preparing a boiler water regulator by a microbial fermentation method comprises the following specific process steps:
(1) pretreatment of raw materials: crushing the enteromorpha and the cotton straws, and sieving the crushed enteromorpha and the crushed cotton straws with a 100-mesh sieve;
(2) preparing materials: accurately mixing sodium tannate, glucose, seaweed, glycol derivatives, cotton straws, starch and a deoxidant according to the proportion of 6.8 parts of sodium tannate, 4 parts of glucose, 9.2 parts of seaweed, 3 parts of glycol derivatives, 4 parts of cotton straws, 2 parts of starch, 1 part of deoxidant and 70 parts of purified water;
(4) mixing: adding the mixed raw materials into purified water through a dosing device, and uniformly mixing;
(5) activating strains: aspergillus niger, yeast and Bacillus subtilis were activated as follows:
the preparation method of the Aspergillus niger seed liquid comprises the following steps: culturing for 24h at 35-40 deg.C in potato culture medium under aseptic condition to obtain Aspergillus niger seed liquid with effective viable count not less than 109cfu/mL；
The preparation method of the yeast seed liquid comprises the following steps: under the aseptic operation condition, two rings of strains are picked from the inclined plane of the yeast YDP by using an inoculating ring, inoculated into a 500mL triangular flask containing 300mLPDA liquid culture medium, subjected to shaking culture at the temperature of between 28 and 32 ℃ and at the speed of 140r/min for 24 hours, and then inoculated into a seed tank for expanded culture according to the process to prepare the yeast seed liquid, wherein the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL; the yeast is a mixed strain of candida utilis and molasses yeast, and the using amount ratio of the yeast is 1: 0.75;
the preparation method of the bacillus subtilis seed solution comprises the following steps: under the aseptic condition, LB culture medium is adopted to culture for 24 hours under the condition of 28-32 ℃ to prepare liquid bacillus seed liquid,the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL；
(6) And (3) microbial fermentation: inoculating the activated aspergillus niger, saccharomycetes and bacillus subtilis to the uniformly mixed material in the step (4) according to the proportion of 3-5%, and performing ventilation fermentation culture for 24-48H at the temperature of 28-35 ℃;
(7) and (3) filtering: filtering the fermented material to remove insoluble substances;
(8) and (4) subpackaging, namely accurately subpackaging the filtered materials to prepare the boiler water treatment agent.
Example 3
A method for preparing a boiler water regulator by a microbial fermentation method comprises the following specific process steps:
(1) pretreatment of raw materials: crushing the enteromorpha and the cotton straws, and sieving the crushed enteromorpha and the crushed cotton straws with a 100-mesh sieve;
(2) preparing materials: accurately mixing sodium tannate, glucose, seaweed, glycol derivatives, cotton straws, starch and a deoxidant according to the proportion of 6.8 parts of sodium tannate, 4 parts of glucose, 9.2 parts of seaweed, 3 parts of glycol derivatives, 4 parts of cotton straws, 2 parts of starch, 1 part of deoxidant and 70 parts of purified water;
(4) mixing: adding the mixed raw materials into purified water through a dosing device, and uniformly mixing;
(5) activating strains: aspergillus niger, yeast and Bacillus subtilis were activated as follows:
the preparation method of the Aspergillus niger seed liquid comprises the following steps: culturing for 24h at 35-40 deg.C in potato culture medium under aseptic condition to obtain Aspergillus niger seed liquid with effective viable count not less than 109cfu/mL；
The preparation method of the yeast seed liquid comprises the following steps: under the aseptic operation condition, two rings of strains are picked from the inclined plane of the yeast YDP by using an inoculating ring, inoculated into a 500mL triangular flask containing 300mLPDA liquid culture medium, subjected to shaking culture at the temperature of between 28 and 32 ℃ and at the speed of 140r/min for 24 hours, and then inoculated into a seed tank for expanded culture according to the process to prepare the yeast seed liquid, wherein the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL; the yeast is a mixture of Candida utilis and molasses yeastThe ratio of the using amount of the bacteria is 1: 0.75;
the preparation method of the bacillus subtilis seed solution comprises the following steps: under the aseptic condition, LB culture medium is adopted to culture for 24 hours at the temperature of 28-32 ℃ to prepare liquid bacillus seed liquid, and the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL；
(6) And (3) microbial fermentation: inoculating the activated aspergillus niger, saccharomycetes and bacillus subtilis to the uniformly mixed material in the step (4) according to the proportion of 3-5%, and performing ventilation fermentation culture for 24-48H at the temperature of 28-35 ℃;
(7) and (3) filtering: filtering the fermented material to remove insoluble substances;
(8) and (4) subpackaging, namely accurately subpackaging the filtered materials to prepare the boiler water treatment agent.
Example 4
A method for preparing a boiler water regulator by a microbial fermentation method comprises the following specific process steps:
(1) pretreatment of raw materials: pulverizing seaweed and cotton straw, and sieving with 100 mesh sieve; the seaweed is a mixture of kelp and enteromorpha in a mass ratio of 1: 1;
(2) preparing materials: accurately mixing sodium tannate, glucose, seaweed, glycol derivatives, cotton straws, starch and a deoxidant according to the proportion of 6.8 parts of sodium tannate, 4 parts of glucose, 9.2 parts of seaweed, 3 parts of glycol derivatives, 4 parts of cotton straws, 2 parts of starch, 1 part of deoxidant and 70 parts of purified water;
(4) mixing: adding the mixed raw materials into purified water through a dosing device, and uniformly mixing;
(5) activating strains: aspergillus niger, yeast and Bacillus subtilis were activated as follows:
the preparation method of the Aspergillus niger seed liquid comprises the following steps: culturing for 24h at 35-40 deg.C in potato culture medium under aseptic condition to obtain Aspergillus niger seed liquid with effective viable count not less than 109cfu/mL；
The preparation method of the yeast seed liquid comprises the following steps: under aseptic condition, two-ring strain is picked from inclined surface of yeast YDP by inoculating ring, and inoculatedInoculating into 500mL triangular flask containing 300mLPDA liquid culture medium, performing shaking culture at 28-32 deg.C for 24 hr at 140r/min, inoculating into seed tank, and performing amplification culture to obtain yeast seed solution with effective viable count of not less than 109cfu/mL; the yeast is candida utilis;
the preparation method of the bacillus subtilis seed solution comprises the following steps: under the aseptic condition, LB culture medium is adopted to culture for 24 hours at the temperature of 28-32 ℃ to prepare liquid bacillus seed liquid, and the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL；
(6) And (3) microbial fermentation: inoculating the activated aspergillus niger, saccharomycetes and bacillus subtilis to the uniformly mixed material in the step (4) according to the proportion of 3-5%, and performing ventilation fermentation culture for 24-48H at the temperature of 28-35 ℃;
(7) and (3) filtering: filtering the fermented material to remove insoluble substances;
(8) and (4) subpackaging, namely accurately subpackaging the filtered materials to prepare the boiler water treatment agent.
Example 5
A method for preparing a boiler water regulator by a microbial fermentation method comprises the following specific process steps:
(1) pretreatment of raw materials: pulverizing seaweed and cotton straw, and sieving with 100 mesh sieve; the seaweed is a mixture of kelp and enteromorpha in a mass ratio of 1: 1;
(2) preparing materials: accurately mixing sodium tannate, glucose, seaweed, glycol derivatives, cotton straws, starch and a deoxidant according to the proportion of 6.8 parts of sodium tannate, 4 parts of glucose, 9.2 parts of seaweed, 3 parts of glycol derivatives, 4 parts of cotton straws, 2 parts of starch, 1 part of deoxidant and 70 parts of purified water;
(4) mixing: adding the mixed raw materials into purified water through a dosing device, and uniformly mixing;
(5) activating strains: aspergillus niger, yeast and Bacillus subtilis were activated as follows:
the preparation method of the Aspergillus niger seed liquid comprises the following steps: culturing in potato culture medium at 35-40 deg.C for 24 hr under aseptic condition to obtain the final productObtaining Aspergillus niger seed liquid with effective viable count not less than 109cfu/mL；
The preparation method of the yeast seed liquid comprises the following steps: under the aseptic operation condition, two rings of strains are picked from the inclined plane of the yeast YDP by using an inoculating ring, inoculated into a 500mL triangular flask containing 300mLPDA liquid culture medium, subjected to shaking culture at the temperature of between 28 and 32 ℃ and at the speed of 140r/min for 24 hours, and then inoculated into a seed tank for expanded culture according to the process to prepare the yeast seed liquid, wherein the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL; the yeast is molasses yeast;
the preparation method of the bacillus subtilis seed solution comprises the following steps: under the aseptic condition, LB culture medium is adopted to culture for 24 hours at the temperature of 28-32 ℃ to prepare liquid bacillus seed liquid, and the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL；
(6) And (3) microbial fermentation: inoculating the activated aspergillus niger, saccharomycetes and bacillus subtilis to the uniformly mixed material in the step (4) according to the proportion of 3-5%, and performing ventilation fermentation culture for 24-48H at the temperature of 28-35 ℃;
(7) and (3) filtering: filtering the fermented material to remove insoluble substances;
(8) and (4) subpackaging, namely accurately subpackaging the filtered materials to prepare the boiler water treatment agent.
Example 6:
a method for preparing a boiler water regulator by a microbial fermentation method comprises the following specific process steps:
(1) pretreatment of raw materials: pulverizing seaweed and cotton straw, and sieving with 100 mesh sieve; the seaweed is a mixture of kelp and enteromorpha in a mass ratio of 1: 1;
(2) preparing materials: accurately mixing 10 parts of sodium tannate, 5 parts of glucose, 12 parts of seaweed, 3 parts of glycol derivative, 5 parts of cotton straw, 2 parts of starch, 1 part of deoxidant and the balance of 62 parts of sodium tannate, glucose, seaweed, glycol derivative, cotton straw, starch and deoxidant in proportion;
(4) mixing: adding the mixed raw materials into purified water through a dosing device, and uniformly mixing;
(5) activating strains: aspergillus niger, yeast and Bacillus subtilis were activated as follows:
the preparation method of the Aspergillus niger seed liquid comprises the following steps: culturing for 24h at 35-40 deg.C in potato culture medium under aseptic condition to obtain Aspergillus niger seed liquid with effective viable count not less than 109cfu/mL；
The preparation method of the yeast seed liquid comprises the following steps: under the aseptic operation condition, two rings of strains are picked from the inclined plane of the yeast YDP by using an inoculating ring, inoculated into a 500mL triangular flask containing 300mLPDA liquid culture medium, subjected to shaking culture at the temperature of between 28 and 32 ℃ and at the speed of 140r/min for 24 hours, and then inoculated into a seed tank for expanded culture according to the process to prepare the yeast seed liquid, wherein the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL; the yeast is a mixed strain of candida utilis and molasses yeast, and the using amount ratio of the yeast is 1: 0.75;
the preparation method of the bacillus subtilis seed solution comprises the following steps: under the aseptic condition, LB culture medium is adopted to culture for 24 hours at the temperature of 28-32 ℃ to prepare liquid bacillus seed liquid, and the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL；
(6) And (3) microbial fermentation: inoculating the activated aspergillus niger, saccharomycetes and bacillus subtilis to the uniformly mixed material in the step (4) according to the proportion of 4%, and performing ventilation fermentation culture for 36H at the temperature of 28-35 ℃;
(7) and (3) filtering: filtering the fermented material to remove insoluble substances;
(8) and (4) subpackaging, namely accurately subpackaging the filtered materials to prepare the boiler water treatment agent.
Example 7 the following tests were used to demonstrate the effect of the invention:
the product of the present application was subjected to a corrosion inhibition coupon test as described in patent application CN 106277380A. In the present application, the corrosion inhibition rate was determined using carbon steel 20, and the density of the test piece was 7.82kg/m3Surface area of 26.9cm2The time is 72 h. The test results are shown in the following table:
table 1 examples 1-6 corrosion inhibition coupon tests
| Examples | Weight loss (g) | Corrosion rate (mm/a) | Inhibition ratio (%) |
| Blank space | 0.09322 | 0.53916 | —— |
| 1 | 0.00433 | 0.02504 | 95.36 |
| 2 | 0.05321 | 0.30776 | 42.92 |
| 3 | 0.04674 | 0.27033 | 50.14 |
| 4 | 0.02032 | 0.11753 | 78.20 |
| 5 | 0.01352 | 0.07820 | 85.50 |
| 6 | 0.00799 | 0.04621 | 85.71 |
From the results of corrosion coupon test data, examples 1-6 have a certain corrosion inhibition effect, wherein the corrosion inhibition rates of the carbon steel coupons of examples 1, 5 and 6 are 95.36%, 85.50% and 85.71%, respectively, and the corrosion inhibition rates are all more than 80%, which indicates that the corrosion inhibition performance is excellent. The corrosion inhibition rates of the embodiment 2 and the embodiment 3 are respectively 42.92% and 50.14%, which shows that the corrosion inhibition performance of the seaweed with a single seaweed source is poor, and this shows that the synergistic effect between the kelp and the enteromorpha remarkably improves the corrosion inhibition effect of the boiler water treatment agent, and obtains the technical effect of 1+1>2, and the analysis reason is probably related to the promotion effect of the seaweed on microorganisms and the molecular polymerization degree of seaweed polysaccharide. The results of example 4 and example 5 show that the selection of the yeast has a significant influence on the action effect of the boiler water treatment agent, and the synergistic effect among the strains can significantly improve the action effect of the boiler water treatment agent. In addition, the corrosion inhibition effect cannot be improved by increasing the addition amount of the raw materials, but the corrosion inhibition effect of the water treatment agent may be reduced. The best example is example 1.
The above embodiments are only used for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; such modifications and substitutions do not depart from the spirit and scope of the present invention as set forth in the appended claims.
Claims (5)
1. A method for preparing a boiler water regulator by a microbial fermentation method is characterized by comprising the following raw materials in parts by weight: 5-10 parts of sodium tannate, 3-5 parts of glucose, 5-12 parts of seaweed, 3 parts of ethylene glycol derivative, 3-5 parts of cotton straw, 2 parts of starch, 1 part of oxygen scavenger and the balance of water, wherein the total weight is 100 parts; the method for preparing the boiler water regulator by the microbial fermentation method specifically comprises the following steps:
(1) pretreatment of raw materials: pulverizing seaweed and cotton straw, and sieving with 100 mesh sieve; the seaweed is a mixture of kelp and enteromorpha in a mass ratio of 1: 1;
(2) preparing materials: accurately mixing sodium tannate, glucose, seaweed, glycol derivatives, cotton straws, starch and a deoxidant in proportion;
(4) mixing: adding the mixed raw materials into purified water through a dosing device, and uniformly mixing;
(5) and (3) microbial fermentation: inoculating the activated aspergillus niger, saccharomycetes and bacillus subtilis to the uniformly mixed material in the step (4) according to the proportion of 3-5%, and performing ventilation fermentation culture for 24-48H at the temperature of 28-35 ℃; the microorganisms comprise Aspergillus niger, microzyme and Bacillus subtilis, and the inoculation ratio is 1:1: 1; the yeast is a mixed strain of candida utilis and molasses yeast, and the using amount ratio of the yeast is 1: 0.75;
(6) and (3) filtering: filtering the fermented material to remove insoluble substances;
(7) and (4) subpackaging, namely accurately subpackaging the filtered materials to prepare the boiler water treatment agent.
2. The method for preparing the boiler water regulator by the microbial fermentation method according to claim 1, wherein the boiler water regulator comprises the following raw materials in parts by weight: 6.8 parts of sodium tannate, 4 parts of glucose, 9.2 parts of seaweed, 3 parts of ethylene glycol derivative, 4 parts of cotton straw, 2 parts of starch, 1 part of deoxidant and 70 parts of purified water.
3. The method for preparing the boiler water regulator by the microbial fermentation method according to any one of claims 1-2, wherein the oxygen scavenger is a mixture of acetaldoxime and diethylhydroxylamine in a mass ratio of 7: 3.
4. The method for preparing boiler water regulator by microbial fermentation according to claim 1, wherein the Aspergillus niger, yeast and Bacillus subtilis strains are activated by the following steps:
the preparation method of the aspergillus niger seed liquid comprises the following steps: culturing for 24h at 35-40 deg.C in potato culture medium under aseptic condition to obtain Aspergillus niger seed liquid with effective viable count not less than 109cfu/mL；
The preparation method of the yeast seed liquid comprises the following steps: under the aseptic operation condition, two rings of strains are picked from the inclined plane of the yeast YDP by using an inoculating ring, inoculated into a 500mL triangular flask containing 300mLPDA liquid culture medium, subjected to shaking culture at the temperature of between 28 and 32 ℃ and at the speed of 140r/min for 24 hours, and then inoculated into a seed tank for expanded culture according to the process to prepare the yeast seed liquid, wherein the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL；
The preparation method of the bacillus subtilis seed solution comprises the following steps: under the aseptic condition, LB culture medium is adopted to culture for 24 hours at the temperature of 28-32 ℃ to prepare liquid bacillus seed liquid, and the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL。
5. A boiler water conditioner prepared by the process of any one of claims 1 to 4.
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