CN108426938A - A kind of direct bioelectrode analytical equipment and analysis method for zymotic fluid detection - Google Patents
A kind of direct bioelectrode analytical equipment and analysis method for zymotic fluid detection Download PDFInfo
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- CN108426938A CN108426938A CN201810491581.1A CN201810491581A CN108426938A CN 108426938 A CN108426938 A CN 108426938A CN 201810491581 A CN201810491581 A CN 201810491581A CN 108426938 A CN108426938 A CN 108426938A
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- 238000004458 analytical method Methods 0.000 title claims abstract description 44
- 239000012530 fluid Substances 0.000 title claims abstract description 24
- 238000001514 detection method Methods 0.000 title claims abstract description 21
- 238000006243 chemical reaction Methods 0.000 claims abstract description 52
- 238000004140 cleaning Methods 0.000 claims abstract description 24
- 239000000872 buffer Substances 0.000 claims abstract description 13
- 238000012360 testing method Methods 0.000 claims abstract description 10
- 239000007853 buffer solution Substances 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 21
- 238000003756 stirring Methods 0.000 claims description 12
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 11
- 230000035484 reaction time Effects 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 8
- 230000006698 induction Effects 0.000 claims description 7
- 238000003825 pressing Methods 0.000 claims description 5
- 239000002699 waste material Substances 0.000 claims description 5
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims description 3
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 claims 1
- 230000004151 fermentation Effects 0.000 abstract description 10
- 238000000855 fermentation Methods 0.000 abstract description 9
- 239000000758 substrate Substances 0.000 abstract description 6
- 238000003908 quality control method Methods 0.000 abstract description 4
- 230000015572 biosynthetic process Effects 0.000 abstract description 3
- 238000012546 transfer Methods 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 27
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- 238000011161 development Methods 0.000 description 3
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- 238000004519 manufacturing process Methods 0.000 description 3
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- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
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- 238000006911 enzymatic reaction Methods 0.000 description 1
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- 235000021393 food security Nutrition 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/38—Cleaning of electrodes
Abstract
A kind of direct bioelectrode analytical equipment and analysis method for zymotic fluid detection, including：Reaction tank, bioelectrode, buffer kit and drain pump.After bioelectrode touches bio-sensing electrode after reaction a period of time, it will produce direct electrode transfer, form faint bioelectrochemistry kinetic current signal, when high into the concentration of substrate to be measured in reaction tank, the kinetic current of formation is also high, and it is linearly proportional within concentration range on one point.Since the working chamber used in reaction tank is the big reaction cavity of capacity, dilute fermentation broth sample concentration, provide the anti-interference ability for changing system, Quality Control, calibration, cleaning etc. are increased simultaneously surveys idle loop section, direct bioelectrode uses high-precision industrial grade Acquisition System for Weak Signal, can be with the Electro Magnetic Compatibility of collecting test system.
Description
Technical field
The present invention relates to biosensor technology fields, and in particular to a kind of direct bioelectrode for zymotic fluid detection
Analytical equipment and analysis method.
Background technology
Biosensor (biosensor) be using biological sensitive materials make recognition component and physics and chemistry energy converter appropriate and
The analysis tool or detecting system that signal amplifying apparatus is constituted.Its core component is bioelectrode, including enzyme electrode, immune electricity
Pole, nucleic acid electrode, cell electrode, tissue electrode etc..It can be widely applied to medicine detection, residential care, food security, industrial mistake
Journey, agriculture application, environment pollution control, military biochemical protection etc..
There are three developing stage for biosensor.By taking electrochemical enzyme electrode as an example, Clark and Lyons is carried for the first time within 1962
The enzyme electrode gone out belongs to first generation biosensor, also referred to as classical enzyme electrode.Its principle is transmitted using the native electron of enzyme
Body ----oxygen links up the electron channel between electrode, directly detects the reduction of enzyme reaction substrate or the generation of product.Second
It is mediator enzyme sensor (mediator enzyme electrode) for biosensor, is to replace oxygen molecule in enzyme using redox electron mediator
Redox active centre and electrode between transmit electronics, solve the dependence to oxygen and the interference problem of electrode activity.The
Three generations's biosensor is also referred to as Direct Electrochemistry enzyme electrode, is that the redox active centre of enzyme and electrode surface directly exchange
The enzyme sensor of electronics.
Direct bioelectrode mostly uses strip and prints electrode, also referred to as reagent strip.It is special with being constantly progressive for manufacturing technology
Be not the development and application of nanotechnology, electrode performance made to be continuously improved, advantageous feature be high sensitivity, strong interference immunity,
Industrialized mass production can be achieved, it is at low cost, it is easy to use etc., thus have been more and more widely used.
Industrial fermentation processes need to carry out fast and accurately fermentation substrate, important mesostate and target product
Detection, to optimize and to control to fermentation process.The characteristics of fermentation broth sample is that sample size is big, batch is more, sample component
Complexity, measured object concentration variation range are wide, and environmental disturbances factor is unstable etc..It is usually that will be tested when reagent strip formula electrode use
Sample is added in electrode surface and is measured, belong to disposable detection method, accuracy and homogenieity by reagent strip manufacturing process,
The influence of storage and transportation mode and storage time cannot meet the requirement of fermented sample high-precision detection.To improve the inspection of reagent strip electrode
The accuracy of survey, many new analytical technologies and analysis product start to apply, and have offshore company to develop Quality Control, calibration, clearly
The analyzer of functions such as wash.But these enzyme electrode analyzers all lay particular emphasis on the measurement of blood sample micro rank, and performance accuracy is wanted
It asks higher, is not suitable for the measurement to fermentation broth sample under industry complex environment.
Invention content
Bioelectrode being connected using reaction tank to overcome the above deficiencies, the invention provides a kind of, realizes matter
Control, calibration, cleaning and one, the strong direct bioelectrode analytical equipment for zymotic fluid detection of anti-electromagnetic interference capability with point
Analysis method.
Technical solution is used by the present invention overcomes its technical problem：
A kind of direct bioelectrode analytical equipment for zymotic fluid detection, including：
Reaction tank is internally provided with working chamber, and the leakage fluid dram and inlet being connected with working chamber are provided on reaction tank；
Bioelectrode is fixed on by fixing device on reaction tank, the buffering liquid phase in the induction zone and working chamber of the bioelectrode
Contact, each signal output end of the bioelectrode are connected to bioelectrode sensing analysis system；
Buffer kit, the liquid feeding end of cleaning pump is connected to by liquid suction pipe II, and outlet end is connected by inlet tube
It is connected to the inlet of reaction tank；And
Drain pump, liquid feeding end are connected to the leakage fluid dram of reaction tank by liquid suction pipe I, and outlet end is connected by drain pipe
In waste liquid box.
Above-mentioned fixing device includes the screw hole being set in reaction tank, and the tail end of the screw hole is connected with working chamber, raw
Object electrode is located in screw hole and is fixed by screwing in the nut in screw hole.
Further include being set on reaction tank and being connected with working chamber to realize stirring evenly for solution on working chamber
Mounting hole II, agitating device are installed in mounting hole II, and agitating device stirs the buffer solution in working chamber.
Further include the mounting hole I for being set on reaction tank and being connected with working chamber, sample introduction to detect after realizing feed liquor
Trigger sensor is installed in mounting hole I, and trigger sensor is connected to bioelectrode sensing analysis system, when being filled in working chamber
When buffer solution, trigger sensor generates trigger signal.
Further include backing plate and the corresponding copper of several and bioelectrode being set on reaction tank to improve leakproofness
Line, the bioelectrode are in contact with backing plate, and circular hole is provided on backing plate, and sealing ring, sealing ring one are provided in the circular hole
End and the induction zone close contact of bioelectrode, the other end and working chamber close contact, on the backing plate with bioelectrode
Each corresponding jack of signal output end, each copper wire are in contact after passing through corresponding jack with corresponding signal output end.
Crushing bioelectrode in order to prevent further includes the pressing plate being set between bioelectrode and nut.
Further, above-mentioned trigger sensor is infrared light-emitting diode and infrared receiving diode, when not having in working chamber
When having buffer solution, infrared receiving diode receives the infrared light that infrared light-emitting diode is sent out, and generates electric signal.
A kind of direct bioelectrode analysis method for zymotic fluid detection includes the following steps：
A) cleaning pump is opened, the buffer solution in buffer kit is pumped into the working chamber of reaction tank, stirring dress by cleaning pump
It sets and stirs and evenly mixs buffer solution；
B) bioelectrode sensing analysis system records bioelectrode measuring electrode current values I at this timeStarting；
C) standard sample is added into working chamber, bioelectrode sensing analysis system starts timing；
D) after the reaction time at T moment, bioelectrode sensing analysis system records bioelectrode measuring electrode at this time
Current values ITerminal；
D) bioelectrode sensing analysis system is according to BDifference=| IStarting-ITerminal| calculate IStartingWith ITerminalDifference absolute value；
E) drain pump unlatching empties working chamber, and cleaning pump, which works at the same time, cleans working chamber；
F) step a)-step d) is repeated, as calculated two groups of BDifferenceBetween error be less than 1%-2% when, execute step
G), as calculated two groups of BDifferenceBetween error be more than 1%-2% when, repeat step a)-step f)；
G) drain pump unlatching empties working chamber, and cleaning pump, which works at the same time, cleans working chamber, and cleaning pump will buffer
Buffer solution in liquid kit is pumped into the working chamber of reaction tank, and agitating device stirs and evenly mixs buffer solution；
H) bioelectrode sensing analysis system records bioelectrode measuring electrode current values I ' at this timeStarting；
I) test sample is added into working chamber, bioelectrode sensing analysis system starts timing；
J) after spending the reaction time at T moment, bioelectrode measuring electrode is electric at this time for bioelectrode sensing analysis system record
Fluxion value I 'Terminal；
K) bioelectrode sensing analysis system is according to formula B 'Difference=| I 'Starting-I’Terminal| calculate I 'StartingWith I 'TerminalDifference
Absolute value；
L) according to formulaCalculate the concentration of test sample, wherein QStandardFor standard sample
Concentration, BCalibrationFor two groups of B in step f)DifferenceOne of.
The beneficial effects of the invention are as follows：After bioelectrode touches bio-sensing electrode after reaction a period of time, it can produce
Direct electrode transfer is given birth to, faint bioelectrochemistry kinetic current signal is formed, the concentration of substrate to be measured in entrance reaction tank
When high, the kinetic current of formation is also high, and within concentration range on one point, linearly proportional.Directly biology
For electrode in biological process industry field, development and application are difficult, are primarily due to that fermentation broth sample amount is big, batch is more, sample
Caused by the factors such as complicated components, measured object concentration variation range are wide, and environmental disturbances factor is unstable.Due to being used in reaction tank
Working chamber be the big reaction cavity of capacity, dilute fermentation broth sample concentration, provide the anti-interference ability for changing system, simultaneously
It increasing Quality Control, calibration, cleaning etc. and surveys idle loop section, direct bioelectrode uses high-precision industrial grade Acquisition System for Weak Signal,
It can make the invention can be with Successful utilization to industrial environment with the Electro Magnetic Compatibility of collecting test system
Description of the drawings
Fig. 1 is the system construction drawing of the present invention；
Fig. 2 is the three-dimensional structure diagram I of the reaction tank of the present invention；
Fig. 3 is the three-dimensional structure diagram II of the reaction tank of the present invention；
In figure, the emptying of 1. reaction tank, 2. agitating device, 3. sample introduction trigger sensor, 4. bioelectrode, 5. working chamber 6.
Pump 7. liquid suction pipe, I 8. drain pipe, 9. waste liquid box, 10. buffer kit, 11. liquid suction pipe, II 12. cleaning pump, 13. feed liquor
20. pressing plate of pipe 14. mounting hole, I 15. mounting hole, II 16. leakage fluid dram, 17. inlet, 18. copper wire, 19. nut, 21. backing plate
22. 23. jack of sealing ring.
Specific implementation mode
Below in conjunction with the accompanying drawings 1, attached drawing 2, the present invention will be further described for attached drawing 3.
A kind of direct bioelectrode analytical equipment for zymotic fluid detection, including：Reaction tank 1, is internally provided with work
Make chamber 5, the leakage fluid dram 16 being connected with working chamber 5 and inlet 17 are provided on reaction tank 1；Bioelectrode 4, passes through fixing device
It is fixed on reaction tank 1, the induction zone of the bioelectrode 4 is in contact with the buffer solution in working chamber 5, the bioelectrode 4
Each signal output end be connected to bioelectrode sensing analysis system；Buffer kit 10 is connected by liquid suction pipe II 11
It is connected to the liquid feeding end of cleaning pump 12, outlet end is connected to the inlet 17 of reaction tank 1 by inlet tube 13；And drain pump 6,
Its liquid feeding end is connected to the leakage fluid dram 16 of reaction tank 1 by liquid suction pipe I 7, and outlet end is connected to waste liquid box 9 by drain pipe 8.
The buffer solution in buffer kit 10 is sent into the working chamber 5 in reaction tank 1 by the work of cleaning pump 12 when work, later in work
Make to make its reaction that can produce after bioelectrode 4 touches bio-sensing electrode after reaction a period of time after sample is added in chamber 5
Direct electrode transfer is given birth to, faint bioelectrochemistry kinetic current signal is formed, the concentration of substrate to be measured in entrance reaction tank 1
When high, the kinetic current of formation is also high, and within concentration range on one point, linearly proportional.Directly biology
For electrode in biological process industry field, development and application are difficult, are primarily due to that fermentation broth sample amount is big, batch is more, sample
Caused by the factors such as complicated components, measured object concentration variation range are wide, and environmental disturbances factor is unstable.Due to being adopted in reaction tank 1
Working chamber 5 is the big reaction cavity of capacity, dilutes fermentation broth sample concentration, provides the anti-interference ability for changing system,
Quality Control, calibration, cleaning etc. are increased simultaneously and surveys idle loop section, and direct bioelectrode is using high-precision industrial grade small-signal acquisition system
System, can make the invention can be with Successful utilization to industrial environment with the Electro Magnetic Compatibility of collecting test system.It is molten after reaction
Liquid can be drained pump 6 and be extracted out from working chamber 5, and be sent into waste liquid box 9, and test next time is facilitated to use.
Preferably, fixing device includes the screw hole being set in reaction tank 1, and the tail end of the screw hole is connected with working chamber
Logical, bioelectrode 4 is located in screw hole and is fixed by screwing in the nut 19 in screw hole.Its mounting structure is simple, operation side
Just.
Can also include the mounting hole II 15 for being set on reaction tank 1 and being connected with working chamber 5, agitating device 2 is installed
In mounting hole II 15, agitating device 2 stirs the buffer solution in working chamber 5.It can will be in working chamber 5 by agitating device 2
Solution stir evenly, improve measure when accuracy.
Can also include the mounting hole I 14 for being set on reaction tank 1 and being connected with working chamber 5, sample introduction trigger sensor 3
It is installed in mounting hole I 14, trigger sensor 3 is connected to bioelectrode sensing analysis system, when being filled with buffer solution in working chamber 5
When, trigger sensor 3 generates trigger signal.Trigger signal is generated by trigger sensor 3, bioelectrode sensing can be controlled
Analysis system starts timing, to facilitate the control reaction time.Further, further include backing plate 21 and be set to reaction tank 1
On several copper wire 18 corresponding with bioelectrode 4, the bioelectrode 4 is in contact with backing plate 21, is provided on backing plate 21
Circular hole is provided with sealing ring 22 in the circular hole, and the induction zone close contact of 22 one end of sealing ring and bioelectrode 4 is another
End and 5 close contact of working chamber, jack 23 corresponding with each signal output end of bioelectrode 4 on the backing plate 21, respectively
A copper wire 18 is in contact after passing through corresponding jack 23 with corresponding signal output end.By increasing backing plate 21 and in backing plate 21
Upper installation sealing ring 22 makes induction zone of the sample solution extremely with bioelectrode 4 in working chamber 5 so as to improve leakproofness
It contacts with each other.Each signal output end of bioelectrode 4 with copper wire 18 by being connected, so as to be convenient for believe using copper wire 18
Number it is transferred to bioelectrode sensing analysis system.
Preferably, further include the pressing plate 20 being set between bioelectrode 4 and nut 19.By in nut 19 and bioelectricity
Pressing plate 20 is set between pole 4, bioelectrode 4 can be prevented by crushing, improve the reliability used.
Specifically, trigger sensor 3 can be infrared light-emitting diode and infrared receiving diode, do not have when in working chamber 5
When having buffer solution, infrared receiving diode receives the infrared light that infrared light-emitting diode is sent out, and generates electric signal.Work as working chamber
After 5 are passed through buffer solution, the infrared light that infrared light-emitting diode is sent out is stopped by buffer solution, and infrared receiving diode can not receive,
So as to generate shutdown, make change in electric, bioelectrode sensing analysis system in working chamber 5 it can be learnt that be passed through slow at this time
Fliud flushing facilitates it to start timing, to help operating personnel to learn the reaction time.
A kind of direct bioelectrode analysis method for zymotic fluid detection includes the following steps：
A) cleaning pump 12 is opened, the buffer solution in buffer kit 10 is pumped into the working chamber of reaction tank 1 by cleaning pump 12
5, agitating device 2 stirs and evenly mixs buffer solution；
B) bioelectrode sensing analysis system records 4 measuring electrode current values I of bioelectrode at this timeStarting；
C) standard sample is added into working chamber 5, bioelectrode sensing analysis system starts timing；
D) after the reaction time at T moment, bioelectrode sensing analysis system records 4 measuring electrode of bioelectrode at this time
Current values ITerminal；
D) bioelectrode sensing analysis system is according to formula BDifference=| IStarting-ITerminal| calculate IStartingWith ITerminalDifference it is absolute
Value；
E) unlatching of drain pump 6 empties working chamber 5, and cleaning pump 12, which works at the same time, cleans working chamber 5；
F) step a)-step d) is repeated, as calculated two groups of BDifferenceBetween error be less than 1%-2% when, execute step
G), as calculated two groups of BDifferenceBetween error be more than 1%-2% when, repeat step a)-step f)；
G) unlatching of drain pump 6 empties working chamber 5, and cleaning pump 12, which works at the same time, cleans working chamber 5, cleaning pump 12
Buffer solution in buffer kit 10 is pumped into the working chamber 5 of reaction tank 1, agitating device 2 stirs and evenly mixs buffer solution；
H) bioelectrode sensing analysis system records 4 measuring electrode current values I ' of bioelectrode at this timeStarting；
I) test sample is added into working chamber 5, bioelectrode sensing analysis system starts timing；
J) after spending the reaction time at T moment, 4 measuring electrode of bioelectrode is electric at this time for bioelectrode sensing analysis system record
Fluxion value I 'Terminal；
K) bioelectrode sensing analysis system is according to formula B 'Difference=| I 'Starting-I’Terminal| calculate I 'StartingWith I 'TerminalDifference
Absolute value；
L) according to formulaCalculate the concentration of test sample, wherein QStandardFor standard sample
Concentration, BCalibrationFor two groups of B in step f)DifferenceOne of.
The concentration of substrate of sample to be tested is calculated by the above method, can be shown by bioelectrode sensing analysis system
Go out, practical simple, standard sample is demarcated before each use, and accuracy of measurement is high, is suitble to third generation bioelectrode application
Micrometric measurement and METHOD FOR CONTINUOUS DETERMINATION.
Claims (8)
1. a kind of direct bioelectrode analytical equipment for zymotic fluid detection, which is characterized in that including：
Reaction tank (1) is internally provided with working chamber (5), the leakage fluid dram being connected with working chamber (5) is provided on reaction tank (1)
(16) and inlet (17)；
Bioelectrode (4) is fixed on by fixing device on reaction tank (1), the induction zone and working chamber of the bioelectrode (4)
(5) buffer solution in is in contact, and each signal output end of the bioelectrode (4) is connected to bioelectrode sensing analysis system
System；
Buffer kit (10), the liquid feeding end of cleaning pump (12) is connected to by liquid suction pipe II (11), and outlet end passes through
Inlet tube (13) is connected to the inlet (17) of reaction tank (1)；And
Drain pump (6), liquid feeding end are connected to the leakage fluid dram (16) of reaction tank (1) by liquid suction pipe I (7), and outlet end passes through
Drain pipe (8) is connected to waste liquid box (9).
2. the direct bioelectrode analytical equipment according to claim 1 for zymotic fluid detection, it is characterised in that：It is described
Fixing device includes the screw hole being set in reaction tank (1), and the tail end of the screw hole is connected with working chamber, bioelectrode (4)
It is fixed in screw hole and by screwing in the nut (19) in screw hole.
3. the direct bioelectrode analytical equipment according to claim 1 for zymotic fluid detection, it is characterised in that：Also wrap
The mounting hole II (15) for being set on reaction tank (1) and being connected with working chamber (5) is included, agitating device (2) is installed on mounting hole
In II (15), agitating device (2) stirs the buffer solution in working chamber (5).
4. the direct bioelectrode analytical equipment according to claim 1 for zymotic fluid detection, it is characterised in that：Also wrap
The mounting hole I (14) for being set on reaction tank (1) and being connected with working chamber (5) is included, sample introduction trigger sensor (3) is installed on peace
It fills in hole I (14), trigger sensor (3) is connected to bioelectrode sensing analysis system, and buffer solution is filled with when working chamber (5) is interior
When, trigger sensor (3) generates trigger signal.
5. the direct bioelectrode analytical equipment according to claim 2 for zymotic fluid detection, it is characterised in that：Also wrap
Several copper wire (18) corresponding with bioelectrode (4) for including backing plate (21) and being set on reaction tank (1), the bioelectricity
Pole (4) is in contact with backing plate (21), and circular hole is provided on backing plate (21), and sealing ring (22), sealing ring are provided in the circular hole
(22) the induction zone close contact of one end and bioelectrode (4), the other end and working chamber (5) close contact, the backing plate
(21) jack (23) corresponding with each signal output end of bioelectrode (4) on, each copper wire (18) pass through corresponding insert
Hole (23) is in contact with corresponding signal output end afterwards.
6. the direct bioelectrode analytical equipment according to claim 2 for zymotic fluid detection, it is characterised in that：Also wrap
Include the pressing plate (20) being set between bioelectrode (4) and nut (19).
7. the direct bioelectrode analytical equipment according to claim 4 for zymotic fluid detection, it is characterised in that：It is described
Trigger sensor (3) is infrared light-emitting diode and infrared receiving diode, infrared when not having buffer solution in the working chamber (5)
Reception diode receives the infrared light that infrared light-emitting diode is sent out, and generates electric signal.
8. a kind of direct bioelectrode analysis method for zymotic fluid detection, which is characterized in that include the following steps：
A) cleaning pump (12) is opened, the buffer solution in buffer kit (10) is pumped into the work of reaction tank (1) by cleaning pump (12)
Make chamber (5), agitating device (2) stirs and evenly mixs buffer solution；
B) bioelectrode sensing analysis system records bioelectrode (4) measuring electrode current values I at this timeStarting；
C) standard sample is added into working chamber (5), bioelectrode sensing analysis system starts timing；
D) after the reaction time at T moment, bioelectrode (4) measuring electrode is electric at this time for bioelectrode sensing analysis system record
Fluxion value ITerminal；
D) bioelectrode sensing analysis system is according to formula BDifference=| IStarting-ITerminal| calculate IStartingWith ITerminalDifference absolute value；
E) drain pump (6), which is opened, empties working chamber (5), and cleaning pump (12), which works at the same time, cleans working chamber (5)；
F) step a)-step d) is repeated, as calculated two groups of BDifferenceBetween error be less than 1%-2% when, execute step g),
As calculated two groups of BDifferenceBetween error be more than 1%-2% when, repeat step a)-step f)；
G) drain pump (6), which is opened, empties working chamber (5), and cleaning pump (12), which works at the same time, cleans working chamber (5), cleaning
Buffer solution in buffer kit (10) is pumped into the working chamber (5) of reaction tank (1) by pump (12), and agitating device (2) will buffer
Liquid stirs and evenly mixs；
H) bioelectrode sensing analysis system records bioelectrode (4) measuring electrode current values I ' at this timeStarting；
I) test sample is added into working chamber (5), bioelectrode sensing analysis system starts timing；
J) after spending the reaction time at T moment, bioelectrode sensing analysis system records bioelectrode (4) measuring electrode electric current at this time
Numerical value I 'Terminal；
K) bioelectrode sensing analysis system is according to formula B 'Difference=| I 'Starting-I’Terminal| calculate I 'StartingWith I 'TerminalDifference it is absolute
Value；
L) according to formulaCalculate the concentration of test sample, wherein QStandardFor the concentration of standard sample,
BCalibrationFor two groups of B in step f)DifferenceOne of.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810491581.1A CN108426938A (en) | 2018-05-21 | 2018-05-21 | A kind of direct bioelectrode analytical equipment and analysis method for zymotic fluid detection |
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| CN117347457A (en) * | 2023-10-07 | 2024-01-05 | 山东谦诺生物科技有限公司 | Automatic calibration system and method for on-line detection of enzyme electrode of bioreactor |
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