CN108279304A - A kind of viral diagnosis device and its application method - Google Patents

A kind of viral diagnosis device and its application method Download PDF

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Publication number
CN108279304A
CN108279304A CN201711486707.8A CN201711486707A CN108279304A CN 108279304 A CN108279304 A CN 108279304A CN 201711486707 A CN201711486707 A CN 201711486707A CN 108279304 A CN108279304 A CN 108279304A
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CN
China
Prior art keywords
box body
lantern ring
diagnosis device
reaction tube
viral diagnosis
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Pending
Application number
CN201711486707.8A
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Chinese (zh)
Inventor
廖明
瞿孝云
徐成刚
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South China Agricultural University
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South China Agricultural University
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Publication date
Application filed by South China Agricultural University filed Critical South China Agricultural University
Priority to CN201711486707.8A priority Critical patent/CN108279304A/en
Publication of CN108279304A publication Critical patent/CN108279304A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983Viruses
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/18Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/34Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents for packaging foodstuffs or other articles intended to be cooked or heated within the package
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D85/00Containers, packaging elements or packages, specially adapted for particular articles or materials
    • B65D85/50Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Evolutionary Biology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The present invention relates to a kind of viral diagnosis device and its application methods, including box body, box body top surface is equipped with box cover, box body both sides are equipped with the first lantern ring by connector, column is equipped in first lantern ring vertically, each column bottom surface is equipped with trapezoidal feet, box bottom both ends, which rotate, is equipped with supporting rod, each supporting rod bottom end is equipped with the second lantern ring, it is horizontal between two column bottom ends to be equipped with guide rod, and second lantern ring be located at guide rod periphery, block is equipped on the inside of column bottom end, guide rod periphery is equipped with second spring, second spring both ends respectively with the second lantern ring on the outside of, it is fixedly connected on the inside of block, box body side is equipped with control mechanism, box body both sides inner wall top is equipped with the first mounting base, it is horizontal between two the first mounting bases to be equipped with mounting plate, several mounting holes are offered in mounting plate.It is easy to operate in the present invention, it is easy to use, detection efficiency is improved, kit is protected not wounded by external force.

Description

A kind of viral diagnosis device and its application method
Technical field
The present invention relates to detection field more particularly to a kind of viral diagnosis device and its application methods.
Background technology
It needs to use detection kit when virus and bacteria detects.But traditional kit buffer capacity force difference, it is easy quilt External force is broken, and another traditional kit does not have heating function, is unfavorable for the detection of virus, causes detection efficiency low.For this purpose, I Propose a kind of virus detection kit and its application method.
Invention content
The purpose of the present invention is to solve disadvantages existing in the prior art, and a kind of virus detection kit proposed And its application method.
To achieve the goals above, present invention employs following technical solutions:
A kind of viral diagnosis device, including box body are designed, the box body top surface is equipped with box cover, and the box body both sides pass through connection Body is equipped with the first lantern ring, is equipped with column in first lantern ring vertically, each column bottom surface is equipped with trapezoidal feet, described Box bottom both ends, which rotate, is equipped with supporting rod, and each supporting rod bottom end is equipped with the second lantern ring, two column bottoms It is horizontal between end to be equipped with guide rod, and the second lantern ring is located at guide rod periphery, and block is equipped on the inside of the column bottom end, it is described to lead It is equipped with second spring to bar periphery, the second spring both ends are fixedly connected on the outside of the second lantern ring, on the inside of block respectively, described Box body side is equipped with control mechanism;
The box body both sides inner wall top is equipped with the first mounting base, and horizontal be equipped with is installed between two first mounting bases Plate offers several mounting holes in the mounting plate, is equipped with reaction tube in each mounting hole, the mounting plate bottom surface is equipped with Multiple auxiliary gussets, and assist being close to the setting of reaction tube side wall on the inside of gusset, it is equipped between installation plate top surface and the reaction tube Air ring, the box cover bottom surface are equipped with the first reagent bag, the second reagent bag and third reagent bag, are placed in the first reagent bag There are NaOH reagents, colour reagent is placed in the second reagent bag, NaCl reagents are placed in the third reagent bag, it is described Tray interior and heating mechanism is equipped at the mounting plate following location directly.
Preferably, the control mechanism control panel, the control panel are arranged on the outer wall of box body side, the control Panel is equipped with display screen and multiple control buttons, and the control panel is surrounded by several connection straps, the connection strap and box External side wall is fixedly connected.
Preferably, the heating mechanism includes electrical bar, and the box body both sides inner wall bottom end is equipped with the second mounting base, institute It states electrical bar to be horizontally set between two the second mounting bases, the box body inner bottom surface is uniformly provided with multiple wind along its length Fan.
Preferably, the box bottom central position is equipped with riser vertically, and riser both sides bottom end is equipped with first The other end and the supporting rod inside of spring, first spring are fixedly connected.
Preferably, the column top is equipped with baffle, and baffle bottom surface both ends are equipped with buffering ball.
Preferably, the box cover top surface is equipped with draw ring, and the box cover central position offers multiple ventholes.
The present invention also provides a kind of application methods of virus detection kit, are as follows:
S1, both hands wear gloves hold draw ring and apply pulling force upwards, take out box cover, then take out reaction tube;
S2, the NaOH solution that 10ml 0.2mol/L are added into reaction tube, the production for the biotin labeling that then addition has expanded Object 5ml adds the NaCl solution of 20ml 0.3mol/L;
After S3, each reaction tube addition reaction raw materials, box cover is covered in box body top surface;
S4, electrical bar and fan are started by control mechanism, temperature in reaction tube is kept to be incubated 10min, then at 50 degrees Celsius High zinc antibody anti-ly is added.
A kind of viral diagnosis device proposed by the present invention and its application method, advantageous effect are:The viral diagnosis device The startup and closing of electrical bar and fan can be controlled by control panel, heating is conducive to reflection raw material and is reacted in reaction tube, By the elongation and shortening of the first spring and second spring, while the first lantern ring is slided up and down along column, and the second lantern ring is along guiding Bar horizontally slips, and plays cushioning effect, easy to operate, easy to use, improves detection efficiency, kit is protected not hit by external force Wound.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram of viral diagnosis device proposed by the present invention;
Fig. 2 is a kind of vertical view of viral diagnosis device proposed by the present invention;
Fig. 3 is a kind of tray interior schematic diagram of viral diagnosis device proposed by the present invention.
In figure:Box body 1, box cover 2, the first lantern ring 3, column 4, trapezoidal feet 5, baffle 6, buffering ball 7, riser 8, supporting rod 9, the first spring 10, guide rod 11, the second lantern ring 12, second spring 13, block 14, control panel 15, connection strap 16, display screen 17, control button 18, draw ring 19, venthole 20, the first mounting base 21, the second mounting base 22, mounting plate 23, reaction tube 24, peace Fill hole 25, auxiliary gusset 26, air ring 27, electrical bar 28, fan 29, the first reagent bag 30, the second reagent bag 31, third reagent Bag 32.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
Referring to Fig.1-3, a kind of viral diagnosis device, including box body 1,1 top surface of box body are equipped with box cover 2, and 2 top surface of box cover is equipped with Draw ring 19,2 central position of box cover offer multiple ventholes 20, and 1 both sides of box body are equipped with the first lantern ring 3 by connector, Column 4 is equipped in first lantern ring 3 vertically, 4 bottom surface of each column is equipped with trapezoidal feet 5, and 4 top of column is equipped with baffle 6, baffle 6 bottom surfaces both ends are equipped with buffering ball 7;The design of draw ring 19 facilitates pull-up box cover 2, the design of venthole 20 to be conducive in box body 1 Air circulation, trapezoidal feet 5 support column 4, and the first lantern ring 3 can be moved up and down along column 4, and baffle 6 and buffering ball 7 are set Meter, avoids the first lantern ring 3 from skidding off 4 top of column, it is ensured that the safety of box body 1.
1 bottom surface both ends of box body, which rotate, is equipped with supporting rod 9, and 9 bottom end of each supporting rod is equipped with the second lantern ring 12, and two vertical It is horizontal between 4 bottom end of column to be equipped with guide rod 11, and the second lantern ring 12 is located at 11 periphery of guide rod, and gear is equipped on the inside of 4 bottom end of column Block 14, the periphery of guide rod 11 are equipped with second spring 13,13 both ends of second spring respectively with 12 outside of the second lantern ring, 14 inside of block It being fixedly connected, 1 bottom surface central position of box body is equipped with riser 8 vertically, and 8 both sides bottom end of riser is equipped with the first spring 10, and first The other end of spring 10 is fixedly connected with 9 inside of supporting rod;Box body 1 moves up and down, and 9 angle of inclination of supporting rod is driven to change, into And the second lantern ring 12 is driven to be moved left and right along guide rod 11, while the first spring 10 and second spring 13 elongate or shorten, simultaneously The design of block 14 avoids the second lantern ring 12 from directly hitting 4 bottom end of column, plays cushioning effect, and then protect box body 1 not straight Connect shock, it is ensured that 1 inside detection operation of box body is normally carried out.
1 side of box body is equipped with control mechanism, control mechanism control panel 15, and control panel 15 is arranged outside 1 side of box body On wall, control panel 15 is equipped with display screen 17 and multiple control buttons 18, and control panel 15 is surrounded by several connection straps 16, Connection strap 16 is fixedly connected with 1 lateral wall of box body;Control panel 15 is mounted on by connection strap 16 on 1 lateral wall of box body, can be led to Startup and closing that control button 18 controls electrical bar 28 and fan 29 are crossed, display screen 17 can show detection progress and related ginseng Number, convenient working personal observations.
1 both sides inner wall top of box body is equipped with the first mounting base 21, and horizontal be equipped with is installed between two the first mounting bases 21 Plate 23 offers several mounting holes 25 in mounting plate 23, is equipped with reaction tube 24 in each mounting hole 25,23 bottom surface of mounting plate is equipped with Multiple auxiliary gussets 26, and the inside of gusset 26 is assisted to be close to the setting of 24 side wall of reaction tube, 23 top surface of mounting plate and reaction tube 24 it Between be equipped with air ring 27,2 bottom surface of box cover be equipped with the first reagent bag 30, the second reagent bag 31 and third reagent bag 32, the first reagent NaOH reagents are placed in bag 30, colour reagent is placed in the second reagent bag 31, NaCl examinations are placed in third reagent bag 32 Agent;Reaction raw materials are added in reaction tube 24, mounting plate 23 plays the role of supporting reaction tube 24, the auxiliary auxiliary of gusset 26 firm Reaction tube 24 avoids reaction tube 24 from toppling over, it is ensured that detection ambient stable.
Heating mechanism is equipped with inside box body 1 and at 23 following location directly of mounting plate, heating mechanism includes electrical bar 28,1 both sides inner wall bottom end of box body is equipped with the second mounting base 22, electrical bar 28 be horizontally set on two the second mounting bases 22 it Between, 1 inner bottom surface of box body is uniformly provided with multiple fans 29 along its length;Electrical bar 28 and fan 29 pass through conducting wire and external electricity Source connects, and starts electrical bar 28 and fan 29, and electrical bar 28 heats its surrounding air, and hot-air is blowed to reaction tube by fan 29 24, and then heat is provided for detection, improve detection efficiency.
The present invention also provides a kind of application methods of viral diagnosis device, are as follows:
S1, both hands wear gloves hold draw ring 19 and apply pulling force upwards, take out box cover 2, then take out reaction tube 24;
S2, the NaOH solution that 10ml 0.2mol/L are added into reaction tube 24, the biotin labeling that then addition has expanded Product 5ml adds the NaCl solution of 20ml 0.3mol/L;
After S3, each reaction tube 24 add reaction raw materials, box cover 2 is covered in 1 top surface of box body;
S4, electrical bar 28 and fan 29 are started by control mechanism, temperature in reaction tube 24 is kept to be incubated at 50 degrees Celsius Then high zinc antibody anti-ly is added in 10min.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto, Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.

Claims (7)

1. a kind of viral diagnosis device, including box body(1), the box body(1)Top surface is equipped with box cover(2), which is characterized in that it is described Box body(1)Both sides are equipped with the first lantern ring by connector(3), first lantern ring(3)It is interior to be equipped with column vertically(4), each The column(4)Bottom surface is equipped with trapezoidal feet(5), the box body(1)Bottom surface both ends, which rotate, is equipped with supporting rod(9), each The supporting rod(9)Bottom end is equipped with the second lantern ring(12), two columns(4)It is horizontal between bottom end to be equipped with guide rod (11), and the second lantern ring(12)It is located at guide rod(11)Periphery, the column(4)Block is equipped on the inside of bottom end(14), described to lead To bar(11)Periphery is equipped with second spring(13), the second spring(13)Both ends respectively with the second lantern ring(12)Outside, block (14)Inside is fixedly connected, the box body(1)Side is equipped with control mechanism;
The box body(1)Both sides inner wall top is equipped with the first mounting base(21), two first mounting bases(21)Between water It is flat to be equipped with mounting plate(23), the mounting plate(23)Inside offer several mounting holes(25), each mounting hole(25)Inside set There is reaction tube(24), the mounting plate(23)Bottom surface is equipped with multiple auxiliary gussets(26), and assist gusset(26)It is close to anti-inside Answer cylinder(24)Side wall is arranged, the mounting plate(23)Top surface and reaction tube(24)Between be equipped with air ring(27), the box cover(2) Bottom surface is equipped with the first reagent bag(30), the second reagent bag(31)With third reagent bag(32), the first reagent bag(30)Inside put It is equipped with NaOH reagents, the second reagent bag(31)Inside it is placed with colour reagent, the third reagent bag(32)Inside it is placed with NaCl reagents, the box body(1)It is internal and be located at mounting plate(23)Heating mechanism is equipped at following location directly.
2. a kind of viral diagnosis device according to claim 1, which is characterized in that the control mechanism control panel (15), the control panel(15)It is arranged in box body(1)On the outer wall of side, the control panel(15)It is equipped with display screen(17) With multiple control buttons(18), the control panel(15)It is surrounded by several connection straps(16), the connection strap(16)And box Body(1)Lateral wall is fixedly connected.
3. a kind of viral diagnosis device according to claim 1, which is characterized in that the heating mechanism includes electrical bar (28), the box body(1)Both sides inner wall bottom end is equipped with the second mounting base(22), the electrical bar(28)It is horizontally set on two Second mounting base(22)Between, the box body(1)Inner bottom surface is uniformly provided with multiple fans along its length(29).
4. a kind of viral diagnosis device according to claim 1, which is characterized in that the box body(1)Bottom surface middle position Place is equipped with riser vertically(8), the riser(8)Both sides bottom end is equipped with the first spring(10), first spring(10)It is another One end and supporting rod(9)Inside is fixedly connected.
5. a kind of viral diagnosis device according to claim 1, which is characterized in that the column(4)Top is equipped with baffle (6), the baffle(6)Bottom surface both ends are equipped with buffering ball(7).
6. a kind of viral diagnosis device according to claim 1, which is characterized in that the box cover(2)Top surface is equipped with draw ring (19), the box cover(2)Central position offers multiple ventholes(20).
7. a kind of a kind of application method of viral diagnosis device according to claim 1-6, which is characterized in that specific steps It is as follows:
S1, both hands wear gloves, hold draw ring(19)Apply pulling force upwards, takes out box cover(2), then take out reaction tube(24);
S2, to reaction tube(24)The NaOH solution of interior addition 10ml 0.2mol/L, then adds the biotin labeling expanded Product 5ml, add the NaCl solution of 20ml 0.3mol/L;
S3, each reaction tube(24)After adding reaction raw materials, in box body(1)Top surface covers box cover(2);
S4, electrical bar is started by control mechanism(28)And fan(29), keep reaction tube(24)Interior temperature is incubated at 50 degrees Celsius 10min is educated, high zinc antibody anti-ly is then added.
CN201711486707.8A 2017-12-30 2017-12-30 A kind of viral diagnosis device and its application method Pending CN108279304A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201711486707.8A CN108279304A (en) 2017-12-30 2017-12-30 A kind of viral diagnosis device and its application method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201711486707.8A CN108279304A (en) 2017-12-30 2017-12-30 A kind of viral diagnosis device and its application method

Publications (1)

Publication Number Publication Date
CN108279304A true CN108279304A (en) 2018-07-13

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CN201711486707.8A Pending CN108279304A (en) 2017-12-30 2017-12-30 A kind of viral diagnosis device and its application method

Country Status (1)

Country Link
CN (1) CN108279304A (en)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1556922A (en) * 1999-08-06 2004-12-22 ֮ Automated point of care detection system including complete sample processing capabilities
WO2005031300A2 (en) * 2003-06-27 2005-04-07 Purdue Research Foundation Device for detecting biological and chemical particles
CN101344404A (en) * 2008-07-31 2009-01-14 苏州德天自动化科技有限公司 Damping device with adjustable elasticity value
CN101379386A (en) * 2005-12-22 2009-03-04 霍尼韦尔国际公司 Portable sample analyzer system
CN105115949A (en) * 2005-12-21 2015-12-02 梅索斯卡莱科技公司 Assay apparatuses, methods and reagents
CN205246671U (en) * 2015-12-18 2016-05-18 西藏民族学院 Hepatitis b virus detect reagent box
CN206281863U (en) * 2016-12-27 2017-06-27 合肥安为康医学检验有限公司 A kind of HPV viruse detection means
CN206334678U (en) * 2016-10-31 2017-07-18 天津铭恒科技发展有限公司 A kind of medical kit of constant temperature high efficiency

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1556922A (en) * 1999-08-06 2004-12-22 ֮ Automated point of care detection system including complete sample processing capabilities
WO2005031300A2 (en) * 2003-06-27 2005-04-07 Purdue Research Foundation Device for detecting biological and chemical particles
CN105115949A (en) * 2005-12-21 2015-12-02 梅索斯卡莱科技公司 Assay apparatuses, methods and reagents
CN101379386A (en) * 2005-12-22 2009-03-04 霍尼韦尔国际公司 Portable sample analyzer system
CN101344404A (en) * 2008-07-31 2009-01-14 苏州德天自动化科技有限公司 Damping device with adjustable elasticity value
CN205246671U (en) * 2015-12-18 2016-05-18 西藏民族学院 Hepatitis b virus detect reagent box
CN206334678U (en) * 2016-10-31 2017-07-18 天津铭恒科技发展有限公司 A kind of medical kit of constant temperature high efficiency
CN206281863U (en) * 2016-12-27 2017-06-27 合肥安为康医学检验有限公司 A kind of HPV viruse detection means

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Application publication date: 20180713