CN108195839A - A kind of image/video processing system and equipment - Google Patents
A kind of image/video processing system and equipment Download PDFInfo
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- CN108195839A CN108195839A CN201810005382.5A CN201810005382A CN108195839A CN 108195839 A CN108195839 A CN 108195839A CN 201810005382 A CN201810005382 A CN 201810005382A CN 108195839 A CN108195839 A CN 108195839A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N30/95—Detectors specially adapted therefor; Signal analysis
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/90—Determination of colour characteristics
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Abstract
The present invention relates to image/video parsing with quantitative analysis detection with reference to field more particularly to a kind of image/video processing system and equipment.The information such as R, G, B, H, S, L, gray scale are resolved out in photo or video frame width, and resolution areas can be that full frame may also mean that and determine region, and the data obtained is subsequently used in the fields such as bioanalysis, chemical analysis, material analysis.Data quantitative result can be presented in the form of three kinds;1 Direct Digital result;The chromatogram that 2 specified image-regions calculate resulting value by row or in row and separately is presented;3 videos specify area data average value to be presented with the chromatogram of video frame width/time shaft.Numeric results can directly display, can also classification results are such as detected, do not detected, content is high, the low written form of content is presented.Chromatogram result has quantitative analysis ability and complicated peak shape processing capacity, and including baseline adjustment, data smoothing, data preserve, adjacent peak is divided, calculated by peak area and saturation peak area are estimated.
Description
Technical field
The present invention relates to computer software and instrument manufacturing more particularly to a kind of image/video processing systems and equipment.
Background technology
With the development of modern science technology, the presentation of more and more health New Sets, food additives causes health
Detection and food security face more acute challenge, become more and more important subject.In recent years, national economy obtains quickly
Development, demand of the people to healthy living quality life are constituted with increasing health, medical treatment, food-safety problem
Strong sends out poor.Wise net report shows that current China's food processing enterprises reach more than 40 ten thousand, only food safety monitoring field
The potential market of analytical instrument is just at 745,000,000,000 yuan or more, and detection consumptive material year market capacity is more than 50,000,000,000 yuan.So quick detection
Technology possesses the solid market demand.Detecting instrument such as mass spectrograph, chromatograph, spectrophotometer etc. used in most of inspections
High end instrument, consumptive material and rapid screening instrument possess broad mass market prospect, while also have abundant profit margin.However it is all in
It is unpractical that layer base or even family, which are all equipped with high end instrument that is expensive and needing cumbersome maintenance, so anxious at present to be developed fast
Detecting instrument is discussed in run-up.One of common quick detection means is exactly thin plate chromatography, life such as chemical analysis using visual determination
The gel electrophoresis of object analysis and ELISA test strip.However these fast inspection technologies face can only qualitative no standard measure the problem of.This
Even if picture or vision signal are converted to number, text results or generate similar chromatography point by invention to solve this problem
From figure as a result, object chromatographic peak is directly detected, so as to provide quantitative testing result.Pertinent instruments are by light path and detection electricity
Special-purpose model is developed in road fine tuning and image analysis and algorithm optimization, the detection of respectively pollutant, toxin, physical signs.
Invention content
The present invention uses camera as detection sensor, since picture or video can transmit many kinds of parameters simultaneously, ginseng
Several statistical dispositions so that accurate quantitative analysis detects.
The present invention is to be achieved by the following technical programs:
A kind of image/video processing system is resolved respectively including a variety of image data R, G, B, H, L, S, gray scale etc.
Come, in being applied for the purpose of analytical test.System can include the acquisition module of image or video, image analysis mould
Block and/or analysis module.
Many kinds of parameters data in image can be parsed.It, being capable of optimized evaluation with reference to chemistry, biology, material experiment data
Algorithm judges fitted signal height, so as to provide testing result.
Numeric results can directly display or present that classification results are such as detected, do not detected, content is high, content is low similar
Written form is presented.Or similar chromatogram is generated as a result, the result has quantitative analysis ability and complicated peak shape processing capacity,
Including baseline adjustment, data smoothing, data preservation, adjacent peak segmentation, calculated by peak area and the estimation of saturation peak area etc.
Function.Wherein the estimation of saturation peak area is to calculate gained using normal distribution equation.
Whole image or video full frame width can be calculated or designated local region is calculated.Show number
Word or text results, chromatogram result can optimize and show including going out the data such as peak position, peak height, peak area, analysis result
It can preserve.
Image data can be handled relies on picture row-column configuration continuously to be regarded as the class chromatogram of X-axis or utilization to generate
Frequency is according to generating using time-dependent value as the chromatogram of X-axis, so as to fulfill continuous monitoring function.The a certain moment specifies the figure in region
As can also further analyze the classification chromatogram generated by picture row-column configuration is X-axis, so as to fulfill the three-dimensional of video image
Analysis.
Data processing is carried out for image obtained by different medium, structure, concentration samples or video, compares by optimization, produces
It is raw for include aflatoxins, blood glucose, uric acid interior different medium analysis fitting algorithm, so as to fulfill object quantitatively or
Semi-quantitative analysis.
A kind of image/video processing equipment, including light source, optical filter and camera.Camera includes the built-in camera shooting of instrument
Head or the combination with mobile phone camera.To needing highly sensitive special analysis, background is lowered using low temperature camera technique and is made an uproar
Sound.
The beneficial effects of the invention are as follows:
The present invention can be used separately as computer software, for analyzing the image collected or video, a variety of image parameters
Optimization calculate and can improve sensitivity and the reliability of result.The present invention can also combine appropriate camera, light source and optical filtering
Piece combines, and develops quick analysis and detecting instrument.The instrument configuration that is developed is simple, analyze speed is fast, easy to operate.
Description of the drawings
Fig. 1 is 1 thin plate chromatography of example and the chromatographed quantitative figure of display analysis of Gel electrophoresis results;Upper figure is thin plate chromatography knot
Fruit, figure below are Gel electrophoresis results.Figure top has shown taken specific region picture.
Fig. 2 is that 2 non-baseline resolved peak of example is quantitatively shown;
Fig. 3 is the standard curve of aflatoxins actual content and optimization algorithm resulting value in a liquid in example 3.
Fig. 4 is directed to the quantitative analysis software of video flowing exploitation.
Specific embodiment
In order to which those skilled in the art is made to more fully understand technical scheme of the present invention, below in conjunction with the accompanying drawings and most
The present invention is described in further detail for good embodiment.
It invents the instrument system being related to and includes computation platform and mobile phone operation platform.System includes light source and optical filtering
Piece, but cell phone platform uses mobile phone camera, and computation platform pertinent instruments need included camera.Instrument is certainly
Band camera uses external power supply, it is possible to use USB powers.High sensitivity should use low temperature camera technique to lower background when requiring
Noise.Image or video can preserve, and also directly unit processing analysis or network can upload progress data analysis.Examination target
May be used the multiple choices such as plastic centrifuge tube, cuvette, glass material container and conduit, quartz material container and conduit or even
Solid fraction or gas detectable substance are in itself.
Image or video frame width can be extracted with full width or default fixed area, can also manually be extracted by operator or
Automatic identification is set, and the shape in specific region can be self-defined.The image data of gained region all pixels point, such as R, G, B, H,
L, S, gray scale etc. are resolved respectively come to carry out subsequent quantitative analysis.Repeatedly quantitatively divided after the acquisition of difference sample data
Analysis optimization, appeal image data is corresponding with sample message such as concentration, medium, respectively development principal component analysis, CV-ANOVA, more
The multinomial statistic op- timizations such as first recurrence, least square method, Partial Least Squares.The quantitative model of optimization is ultimately generated, it is then default
Enter analysis software.
When image analysis result is directly represented by numeric results or text results, whole pixels of image or video frame width
Point or all pixels dot image data in designated analysis region are resolved, and mean value quilt simultaneously brings the quantitative model after optimization into, finally
Generate analysis result.
When image analysis result is shown by chromatogram, image or whole pixels of video frame width or designated analysis region
All pixels dot image data can be counted respectively row wise or column wise, row number ranking or line number ranking are on chromatogram with X-axis
It representing, the mean value of corresponding one's own profession or the parameters such as this row all R, G, B, H, L, S, gray scale is brought into the quantitative model after optimization,
Generate one's own profession or the Y-axis data of this row.All row or column data are depicted as Two way chromatograms figure.For the chromatogram generated
Shape can carry out the processing such as baseline zero, baseline adjustment, negative value display posivtive spike, data smoothing so that peak shape and the smooth U.S. of baseline
It sees.Quantitative analysis first has to define peak, and for the single peak shape of standard, peak start bit and stop bits are established using clicking twice, with
Afterwards using calculated by peak area formula, calculate trapezoidal area between start bit and stop bits between all each two peak values and baseline it
Maximum value and the difference of baseline value between start bit and stop bits are used with, peak value.In thin plate chromatography or Gel electrophoresis results figure
In, often there is adjacent peak separation and be not thorough, the situation of baseline separation can not be completed.Such case gives a definition peak using three times or more
Secondary click establishes each peak start bit and stop bits with the effective range at clear and definite each peak, subsequent peak height and calculated by peak area side
Method is the same as standard simple spike.In addition, the present invention is also carried for saturation calculated by peak area common in thin plate chromatography or Gel electrophoresis results
Supplied computational methods, here saturation peak refer to analyte concentration it is excessively high when reach the flat peak, blunt that the upper limit of detector feedback occurs
Situations such as circle peak.The present invention, using each peak start bit and stop bits, that is to say the pass of peak width and peak shape for such case
System, calculates the complete peak shape of virtual mesokurtosis, and then calculate virtual peak area.
The present invention also provides scheme to the parsing of video flowing.After video signal collection, the full width of each frame width or refer to
Determine the parameters such as the R of the pixel in region, G, B, H, L, S, gray scale to be used to be calculated.Specify region can be with manual identification here
Also can be according to requirement of experiment automatic identification, the shape in specific region can be self-defined.Full width or specified region so pixel,
Or generated using continuous video data using time-dependent value as the chromatogram of X-axis, so as to fulfill continuous monitoring function.With the time
Correlation is the quantitative calculation method of the chromatogram of X-axis with above.It is noted that specify region along the time shaft a certain moment
Image can also further analyze generate by picture row-column configuration be X-axis classification chromatogram, so as to make the three of video image
Dimension analysis is possibly realized.
In appeal process, picture, video, analysis result data, chromatogram serial number, chromatogram quantification result figure can under
It carries and preserves.Shown in addition, the present invention also provides chromatogram for image or the single parameters such as video R, G, B, H, L, S, gray scale, for
Family intuitively understands each parameter to the contribution of detection signal and the correlativity of also detectable substance, this group of numerical value and picture can also preserve.
The technical solution in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example is only the part of the embodiment of the present invention, instead of all the embodiments.These embodiments are given as examples, and
Limit the scope of the present invention with being not intended to.These embodiments can be implemented, and in a manner that others are various not
It is detached from the range of the purport of the present invention, various omissions can be carried out, replace and change.Based on the embodiments of the present invention,
All other embodiment that those of ordinary skill in the art are obtained without making creative work, belongs to this hair
The range of bright protection.
The chromatographed quantitative analysis of 1 picture of example.For thin plate chromatography and Gel electrophoresis results figure, analysis system is brought into respectively
System.After calculating the optimization of the pixels such as R, G, B, H, L, S, gray scale, optimal value is obtained by column, using row number as X-axis, chromatographed point
It analyses the display of result and quantifies.
Example 2 is with the thin plate chromatography map analysis of example 1, and when adjacent two peak is not up to baseline separation, swarming method is by success
Implement the peak area and peak height to quantify two peaks respectively.
3 ultraviolet source of example is used as excitation light source, and the sample containing aflatoxins is irradiated after light path converges.Sample
1.5 milliliters of plastic centrifuge tubes are positioned over, diverging light is after intercepting the optical filter of less than 400 nanometers light, by mobile phone camera
Acquisition.Each parameter value of pixel of gained image is by principal component analysis, CV-ANOVA, multiple regression, partially least square method, minimum
Optimal algorithm is generated after the multinomial statistical analysis optimization such as square law.Final algorithm can perfection to agree with detection aflatoxins actually dense
Degree.
Example 4 is directed to the quantitative analysis software of video flowing exploitation.The software can acquire preservation video, to specified region into
Row image analysis simultaneously draws chromatogram using the time as Y-axis.Each parameter to the contribution Distribution value of result and quantitative point final
Analysis result can be shown with image result.
Every the equivalent structure or equivalent process transformation made by using the contents of the present specification, is directly or indirectly used
In other relevant technical fields, it is included within the scope of the present invention.
Claims (7)
1. a kind of image/video processing system, it is characterised in that including a variety of image data R, G, B, H, L, S, gray scale etc. respectively by
It parses, in being applied for the purpose of analytical test, system can include the acquisition module of image or video, image
Analysis module and/or analysis module.
2. a kind of image/video processing system as described in claim 1, it is characterised in that:
Many kinds of parameters data in image can be parsed, with reference to chemistry, biology, material experiment data, can optimized evaluation algorithm,
Fitted signal height is judged, so as to provide testing result.
3. a kind of image/video processing system as described in claim 1, it is characterised in that:
Numeric results can directly display or present that classification results are such as detected, do not detected, content is high, the low similar word of content
Form presents or generates similar chromatogram as a result, the result has quantitative analysis ability and complicated peak shape processing capacity, wherein
Including baseline adjustment, data smoothing, data preserve, adjacent peak segmentation, calculated by peak area and saturation peak area estimation etc. functions,
Wherein the estimation of saturation peak area is to calculate gained using normal distribution equation.
4. a kind of image/video processing system as claimed in claim 3, it is characterised in that:
Whole image or video full frame width can be calculated or designated local region is calculated.Displaying number or
Text results, chromatogram result can optimize and show including going out the data such as peak position, peak height, peak area, and analysis result can be with
It preserves.
5. a kind of image/video processing system as claimed in claim 3, it is characterised in that:
Image data can be handled and rely on picture row-column configuration as the class chromatogram of X-axis or utilization continuous videos number to generate
According to generation using time-dependent value as the chromatogram of X-axis, so as to fulfill continuous monitoring function, a certain moment specifies the image in region also
The classification chromatogram generated by picture row-column configuration is X-axis can be further analyzed, so as to fulfill the three dimensional analysis of video image.
6. a kind of image/video processing system as described in claim 1, it is characterised in that:
Data processing is carried out for image obtained by different medium, structure, concentration samples or video, compares by optimization, generates needle
To include aflatoxins, blood glucose, uric acid interior different medium analysis fitting algorithm, so as to fulfill the quantitative or semidefinite of object
Amount analysis.
7. a kind of image/video processing equipment, it is characterised in that:
Including light source, optical filter and camera, camera includes instrument built-in camera or the combination with mobile phone camera,
To needing highly sensitive special analysis, background noise is lowered using low temperature camera technique.
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2018
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CN103649731A (en) * | 2011-03-31 | 2014-03-19 | 阿尔巴盖亚有限公司 | Testing apparatus |
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