CN108169285B - Method for measuring content of soluble sugar in kalopanax pohuashanensis by adopting electrical impedance loss coefficient delta value method - Google Patents
Method for measuring content of soluble sugar in kalopanax pohuashanensis by adopting electrical impedance loss coefficient delta value method Download PDFInfo
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Abstract
The invention provides a method for measuring the content of soluble sugar in sorbus pohuashanensis by an electrical impedance loss coefficient delta method, which comprises the following steps: detecting the resistance value and the volume resistance value of a sample to be detected of the kalopanax pohuashanensis, and drawing an electrical impedance map; determining a peak value suitable for calculating an impedance loss coefficient delta according to the electrical impedance map of the sample to be detected; calculating an impedance loss coefficient delta value formed by a resistance value and a capacitive reactance value according to the proper peak value; and establishing a regression model of the resistance loss coefficient delta value and the soluble sugar content of the sorbus pohuashanensis, and obtaining the soluble sugar content in the sorbus pohuashanensis stem according to the regression model and the resistance loss coefficient delta value of the sample to be detected. The method provided by the invention establishes a regression model between the electrical impedance loss coefficient delta of the mountain ash and the soluble sugar content of the mountain ash, so that the soluble sugar content in the mountain ash stem is calculated according to the measured electrical impedance loss coefficient delta, and the rapid and real-time diagnosis of the soluble sugar content can be realized.
Description
Technical Field
The invention relates to the technical field of plant component content detection, in particular to a method for determining the content of soluble sugar in sorbus pohuashanensis by an electrical impedance loss coefficient delta value method.
Background
The adaptation of plants to low temperature stress is closely related to physiological and biochemical activities such as cell membranes, enzyme system protection, osmoregulation substances and the like. The simple principle is that the plant is mainly subjected to osmotic regulation by soluble sugar in the early stage of low-temperature stress, and other osmotic substances such as soluble protein and the like act when the stress is further deepened, so that the content of the soluble sugar is often used as a physicochemical index for identifying the cold resistance of the plant. The method can be used for timely and accurately detecting and diagnosing the content change of the soluble sugar in the plant body, is beneficial to understanding the physiological condition change of the plant, and has important significance for monitoring the cold resistance of the plant.
The Catalpa spp is a special germplasm resource in China, is an important tree species for use not only in urban greening, but also in important materials, and has wide application approaches. The catalpa bungei elements are named as 'wood king', are distributed in wide areas of the eastern seashore, the west to Gansu, the south to Yunnan and the north to the great wall, are pleased with light and cold, have the average temperature of 10-15 ℃ in the growth year, can build a fast-growing and high-yield forest to obtain high-quality materials, can also be used as a protection forest for preserving water and soil, can be used as an urban greening tree species, and have strong pollution resistance and high ornamental value. Along with the increase of the greening consciousness of people, the catalpa bungei as an excellent ornamental tree species can be widely applied to greening in the north.
In order to study the adaptability of the kalopanax septemlobus to northern temperature, the cold resistance of the kalopanax septemlobus needs to be measured. In view of the relationship between the soluble sugar content and the cold resistance found in the prior art, the detection of the soluble sugar content can realize the determination of the cold resistance of the kalopanax pohuashanensis. The traditional method for measuring the plant soluble sugar mainly comprises an anthrone method and a phenol method, but the traditional method is not only tedious, labor-consuming and time-consuming, but also has destructiveness and hysteresis, and cannot meet the requirements of rapidness and real-time in production.
Disclosure of Invention
The invention aims to provide a method for measuring the content of soluble sugar in the sorbus pohuashanensis by an impedance loss coefficient delta value method, and the method provided by the invention can realize the rapid and real-time measurement of the content of soluble sugar in the sorbus pohuashanensis.
The invention provides a method for measuring the content of soluble sugar in sorbus pohuashanensis by an electrical impedance loss coefficient delta value method, which comprises the following steps:
detecting the resistance value and the volume resistance value of a sample to be detected of the kalopanax pohuashanensis, and drawing an electrical impedance map;
determining a peak value suitable for calculating an impedance loss coefficient delta according to the electrical impedance map of the sample to be detected;
calculating an impedance loss coefficient delta value formed by a resistance value and a capacitive reactance value according to the peak value;
and establishing a regression model of the resistance loss coefficient delta value and the soluble sugar content of the sorbus pohuashanensis, and obtaining the soluble sugar content of the sorbus pohuashanensis according to the regression model and the resistance loss coefficient delta value of the sample to be detected.
Preferably, the peak value is at a frequency point of 12KHz or 15 KHz.
Preferably, in the regression model of the ash resistance loss coefficient delta value and the soluble sugar content, the ash resistance loss coefficient delta value is an independent variable, and the soluble sugar content is a dependent variable.
Preferably, the sample to be detected of the kalopanax septemlobus is kalopanax septemlobus stems.
Preferably, the regression model of the resistance loss coefficient delta value and the soluble sugar content of the kalopanax pohuashanensis stem is a linear equation, a quadratic equation or a cubic equation.
Preferably, when the regression model of the resistance loss coefficient delta value and the soluble sugar content of the kalopanax pohuashanensis stem is a linear equation, the linear equation is as follows: y is-0.447 delta +0.038, wherein delta is the impedance loss coefficient delta of the kalopanax pohuashanensis stem, and y is the soluble sugar content.
Preferably, when the regression model of the resistance loss coefficient delta value and the soluble sugar content of the kalopanax septemlobus stem is a quadratic equation, the quadratic equation is as follows: y is-0.017 delta2-0.460 δ +0.036, where δ is the impedance loss coefficient δ of the kalopanax pohuashanensis stem and y is the soluble sugar content.
Preferably, when the regression model of the resistance loss coefficient delta value and the soluble sugar content of the kalopanax pohuashanensis stem is a cubic equation, the cubic equation is as follows: y is-18.67 delta3-20.47δ27.697 delta-0.790, where delta is the impedance loss coefficient delta of kalopanax pohuashanensis stem and y is the soluble sugar content.
The invention provides a method for measuring the content of soluble sugar in a Chinese ash by using an electrical impedance loss coefficient delta value method, which comprises the following steps: detecting the resistance value and the volume resistance value of a sample to be detected of the kalopanax pohuashanensis, and drawing an electrical impedance map; determining a peak value suitable for calculating an impedance loss coefficient delta according to the electrical impedance map of the sample to be detected; calculating an impedance loss coefficient delta value formed by a resistance value and a capacitive reactance value according to the peak value; and establishing a regression model of the resistance loss coefficient delta value and the soluble sugar content of the sorbus pohuashanensis, and obtaining the soluble sugar content of the sorbus pohuashanensis according to the regression model and the resistance loss coefficient delta value of the sample to be detected. The method provided by the invention establishes a regression model between the delta value of the impedance loss coefficient of the sorbus pohuashanensis and the content of the soluble sugar in the sorbus pohuashanensis, so that the content of the soluble sugar in the sorbus pohuashanensis is calculated according to the delta value of the measured impedance loss coefficient, and the content of the soluble sugar can be rapidly diagnosed in real time. Because the content of the soluble sugar reflects the cold resistance of the kalopanax pohuashanensis to a certain extent, the method for measuring the content of the soluble sugar in the kalopanax pohuashanensis by using the electrical impedance loss coefficient delta value method can obtain the cold resistance of the kalopanax pohuashanensis according to the change of the impedance loss coefficient delta value, and provides important theoretical basis and technical support for the estimation of the change of the cold resistance.
Drawings
FIG. 1 is a graph showing the change of electrical impedance spectroscopy of the stem of Sorbus pohuashanensis obtained in example 1 of the present invention;
FIG. 2 is a graph showing the variation of the soluble sugar content in the stem of kalopanax septemlobus during cold-resistant exercise according to example 4 of the present invention.
Detailed Description
The invention provides a method for measuring the content of soluble sugar in sorbus pohuashanensis by an electrical impedance delta value method, which comprises the following steps:
detecting the resistance value and the volume resistance value of a sample to be detected of the kalopanax pohuashanensis and drawing an electrical impedance map;
determining a peak value suitable for calculating an impedance loss coefficient delta according to the electrical impedance map of the sample to be detected;
calculating an impedance loss coefficient delta value formed by a resistance value and a capacitive reactance value according to the peak value;
and establishing a regression model of the resistance loss coefficient delta value and the soluble sugar content of the sorbus pohuashanensis, and obtaining the soluble sugar content of the sorbus pohuashanensis according to the regression model and the resistance loss coefficient delta value of the sample to be detected.
The method provided by the invention establishes a regression model between the delta value of the impedance loss coefficient of the sorbus pohuashanensis and the content of the soluble sugar in the sorbus pohuashanensis, so that the content of the soluble sugar in the sorbus pohuashanensis is calculated according to the delta value of the measured impedance loss coefficient, and the content of the soluble sugar can be rapidly diagnosed.
The invention detects the electrical impedance atlas of the detected sample of the kalopanax septemlobus. The selection of the sample to be tested is not particularly limited in the present invention, and a representative sample to be tested, such as a stem of kalopanax septemlobus, can be selected by a person skilled in the art according to a method well known to those skilled in the art. In the embodiment of the present invention, the length of the sample to be tested of the kalopanax septemlobus stem is preferably 10mm to 30mm, and more preferably 15mm to 20 mm. In the embodiment of the present invention, the kalopanax septemlobus stem is preferably used as the sample to be tested.
The apparatus for measuring the electrical impedance is not particularly limited, and an electrical impedance apparatus known to those skilled in the art may be used. In an embodiment of the invention, the impedance meter may be a model HP4284A impedance meter. In the invention, the electrode used for measuring the electrical impedance is preferably an Ag/AgCl electrode; the present invention preferably coats the electrode gel on the electrode surface. In the present invention, the coating thickness of the electrode gel is preferably 0.5cm to 2cm, more preferably 1cm to 1.5 cm. The source and type of the electrode and electrode GEL are not particularly limited in the present invention, and commercially available products of electrodes and electrode GELs for electrical impedance measurement known to those skilled in the art may be used, for example, RCI type electrodes manufactured by WPI manufactured in the United states and GEL100 type GELs manufactured by WPI manufactured in the United states.
When a sample to be detected of the kalopanax septemlobus stem is detected, the electrode is preferably contacted with the cross section of the sample to be detected; when the electrode gel is coated on the surface of the electrode, the cross section of the sample to be tested is preferably contacted with the electrode gel.
After obtaining the electrical impedance atlas of the sample to be measured, the invention determines the peak value suitable for calculating the impedance loss coefficient delta according to the electrical impedance atlas of the sample to be measured, and in the invention, the optimal peak value is preferably at the frequency point of 12KHz or 15 KHz. And calculating an impedance loss coefficient delta value formed by the resistance value and the capacitive reactance value according to the peak value. The method specifically comprises the following steps:
the calculation formula of the electrical impedance is shown in formula I:
in the formula I, ZImIs a capacitive reactance value, ZReIs a resistance value;
δ is the impedance loss coefficient.
After the resistance loss coefficient delta value is obtained, the soluble sugar content in the kalopanax pohuashanensis is obtained according to a preset regression model of the resistance loss coefficient delta value and the soluble sugar content and the resistance loss coefficient delta value of the sample to be detected. The research of the invention finds that the impedance loss coefficient delta of the stem sum of the kalopanax pohuashanensis and the content of soluble sugar in the kalopanax pohuashanensis have higher correlation, so that a regression model between the content of the soluble sugar and the value of the impedance loss coefficient delta is established.
In the invention, in the regression model of the electrical impedance loss coefficient delta value and the soluble sugar content of the kalopanax pohuashanensis, the electrical impedance loss coefficient delta value is an independent variable, and the soluble sugar content is a dependent variable; in the embodiment of the invention, the regression model of the resistance loss coefficient delta value and the soluble sugar content of the kalopanax septemlobus stem is a linear equation, a quadratic equation, a cubic equation or an exponential equation.
In the invention, the regression model of the resistance loss coefficient delta value and the soluble sugar content of the kalopanax pohuashanensis stem is preferably established according to the following method:
measuring the content of soluble sugar in the catalpa bungei;
detecting the resistance value and the volume resistance value of a sample to be detected of the kalopanax pohuashanensis, and drawing an electrical impedance map;
determining a peak value suitable for calculating an impedance loss coefficient delta according to the electrical impedance map of the sample to be detected;
and calculating an impedance loss coefficient delta value formed by the resistance value and the capacitive reactance value according to the proper peak value.
And performing regression analysis on the soluble sugar content of the sorbus pohuashanensis and the impedance loss coefficient delta value to obtain a regression model of the sorbus pohuashanensis impedance loss coefficient delta value and the soluble sugar content. The invention detects the content of soluble sugar in the sorbus pohuashanensis. The method for detecting the content of the soluble sugar is not particularly limited, and the kalopanax septemlobus stems can be detected by adopting a technical scheme for detecting the content of the soluble sugar, which is well known to a person skilled in the art. In the examples of the present invention, the content of soluble sugar in sorbus pohuashanensis can be measured by anthrone color development. In the invention, before the measurement of the kalopanax septemlobus stems, the kalopanax septemlobus stems are preferably cleaned and dried; the cleaning method is not particularly limited, and a method for cleaning a plant sample, which is well known to those skilled in the art, may be used, for example, deionized water may be used to clean the stem of kalopanax septemlobus, and then the stem is dried after being wiped. The drying method is not particularly limited, and the technical scheme of drying known to the skilled person can be adopted; in the invention, the drying equipment is preferably an oven; the drying temperature is preferably 70-100 ℃, more preferably 75-90 ℃, and most preferably 80-85 ℃; the drying time is preferably 45h to 3d, and more preferably 2d to 2.5 d.
In the invention, the resistance loss coefficient delta value of the kalopanax pohuashanensis stem is obtained by preferably measuring according to the method for measuring the electrical impedance.
After the soluble sugar content of the kalopanax pohuashanensis and the impedance loss coefficient delta value of the kalopanax pohuashanensis are obtained, the invention carries out correlation analysis on the soluble sugar content and the impedance loss coefficient delta value. The impedance loss coefficient delta value is proved to have better correlation with the soluble sugar of the kalopanax pohuashanensis, and the correlation coefficient reaches-0.957 and-0.914.
The invention performs regression analysis on the soluble sugar content and the impedance loss coefficient delta value of the sorbus pohuashanensis to obtain a regression model of the sorbus pohuashanensis impedance loss coefficient delta value and the soluble sugar content. In the present invention, the regression analysis may be linear regression analysis or nonlinear regression analysis; the non-linear regression analysis preferably includes a logarithmic regression analysis, a quadratic regression analysis, a cubic regression analysis, and an exponential regression analysis.
In the examples of the present invention, the obtained line of the stem of kalopanax septemlobusThe sexual regression model is that y is-0.447 delta + 0.038; the second regression model of the kalopanax pohuashanensis stem is that y is-0.017 delta2-0.460 δ + 0.036; the cubic regression model of the kalopanax pohuashanensis stem is that y is-18.67 delta3-20.47δ2-7.697 δ -0.790; the exponential regression model of the kalopanax septemlobus stem is that y is 0.086e-2.23δ(ii) a In the regression model of kalopanax pohuashanensis stems, yStem of a treeIs the content of soluble sugar in the kalopanax septemlobus stem, and delta is the impedance loss coefficient.
After the regression model of the resistance loss coefficient delta value and the soluble sugar content of the sorbus pohuashanensis is obtained, the soluble sugar content of the sorbus pohuashanensis is calculated according to the resistance loss coefficient delta value of the sample to be detected obtained by the technical scheme and the regression model. The regression models of the sorbus pohuashanensis are respectively established by the method provided by the invention, so that the content of the soluble sugar in the sorbus pohuashanensis is obtained.
The method provided by the invention establishes a regression model between the electrical impedance loss coefficient delta value and the soluble sugar content, and the electrical impedance loss coefficient delta value is irrelevant to the size of a sample, so that only the measurement error needs to be considered, the objective state of the plant can be reflected, and the accuracy of the measurement result is high.
In the prior art, the content of soluble sugar is often used as a physicochemical index for identifying the cold resistance of plants, so that the change of the content of soluble sugar in the plants can be timely and accurately detected and diagnosed, and the change of the physiological condition of the plants can be known. Therefore, the method provided by the invention can detect the content of the soluble sugar, can reflect the cold resistance of the kalopanax pohuashanensis, and can be directly used for analyzing the cold resistance of the kalopanax pohuashanensis.
The invention provides a method for measuring the content of soluble sugar in sorbus pohuashanensis by an electrical impedance loss coefficient delta value method, which comprises the following steps: detecting the resistance value and the volume resistance value of a sample to be detected of the kalopanax pohuashanensis, and drawing an electrical impedance map; determining a peak value suitable for calculating an impedance loss coefficient delta according to the electrical impedance map of the sample to be detected; calculating an impedance loss coefficient delta value formed by a resistance value and a capacitive reactance value according to the proper peak value; and establishing a regression model of the resistance loss coefficient delta value and the soluble sugar content of the sorbus pohuashanensis, and obtaining the soluble sugar content of the sorbus pohuashanensis according to the regression model and the resistance loss coefficient delta value of the sample to be detected. The method provided by the invention establishes a regression model between the delta value of the impedance loss coefficient of the sorbus pohuashanensis and the content of the soluble sugar in the sorbus pohuashanensis, so that the content of the soluble sugar in the sorbus pohuashanensis is calculated according to the delta value of the measured impedance loss coefficient, and the content of the soluble sugar can be rapidly diagnosed in real time. Because the content of the soluble sugar reflects the cold resistance of the kalopanax pohuashanensis to a certain extent, the method for measuring the content of the soluble sugar in the kalopanax pohuashanensis by using the impedance loss coefficient delta value method can obtain the cold resistance of the kalopanax pohuashanensis according to the change of the impedance loss coefficient delta value, and provides important theoretical basis and technical support for the estimation of the change of the cold resistance.
For further illustration of the present invention, the method for determining the content of soluble sugar in kalopanax septemlobus by the delta value of impedance loss provided by the present invention will be described in detail with reference to the following examples, which should not be construed as limiting the scope of the present invention.
The experiments of the following examples were conducted from 9 to 1 month in 2009, with 1 year old grafted seedlings as test materials, and mountain ash of hongkong, jiangsu (119.16 'N34.59' E), transplanted to specimen garden of north Hei agriculture university (3850 'N, 11526' E) at 28 days 4 and 28 days in 2009, and stems were sampled every other month from 20 days 9 to 20 days 1 and 2010 in 2009, when mountain ash enters into cold-resistant exercise.
Example 1
Cleaning and wiping the stems with deionized water, placing into plastic bags, repeating for 8 times, and spraying small amount of deionized water to prevent supercooling of the material. The freezing treatment temperature was set to 7 temperature gradients (see table 1) with a control of 4 ℃ containing the temperature of the sample at which all survivors and all deaths occurred. The cooling rate is 6 ℃ h-1And keeping for 4h when the treatment temperature is reached, then unfreezing for 24h at 4 ℃ and testing.
TABLE 1 determination of Cold resistance of Liriodendron bungei stems in example 1 of the present invention freezing treatment set temperature
Randomly selecting 8 sections of thawed stems with the length of 15mm for electrical impedance measurement:
an impedance measuring instrument for EIS (HP4284A, USA) adopts Ag/AgCl electrode, electrode gel is added on the electrode, the cross section of the sample is contacted with the gel, and the electrical impedance spectrum of the stem of kalopanax septemlobus is measured.
As shown in FIG. 1, FIG. 1 is a graph showing the variation of the electrical impedance spectrum of the stem of Kalopanax pictus obtained in example 1 of the present invention, the spectrum is the average value of the processing data (42 frequencies from 80Hz to 1MHz from right to left), and it can be seen from FIG. 1 that the EIS of the stem of Kalopanax pictus is changed during the exercise of cold resistance, indicating that the internal structure and physiological and biochemical processes of the stem are changed correspondingly to adapt to the environmental changes. The stem atlas shows a single arc, the arc of the stem gradually increases along with the process of cold-resistant exercise, and the arc top reactance value of the stem tends to decrease all the time. The peak value of the electrical impedance atlas is at the frequency point of 12KHz or 15 KHz.
Example 2
Cleaning and wiping the stems of the sorbus pohuashanensis with deionized water, drying in an oven at 80 ℃ for 2d, taking out, and measuring the content of soluble sugar in the sorbus pohuashanensis by adopting an anthrone color development method;
the impedance values of 75 groups of samples of the kalopanax septemlobus stems were measured according to the protocol of example 1 to obtain the impedance values of the 75 groups of kalopanax septemlobus stem samples, and the impedance loss coefficient delta value was measured by referring to the method of "green technology", 2010, (9): 145-148 ") for the relationship between the impedance parameters and the temperature in the plants of marangxiang and dawn yang.
Processing and variance analysis are carried out on test data by adopting SPSS22.00 software, data analysis is carried out by adopting SPSS22.0 statistics and Excel2010, and a repeated measurement analysis method is adopted for difference significance analysis; the accuracy and applicability of the estimation model adopt a universal determining coefficient (R)2) Root Mean Square Error (RMSE) and Relative Error (RE)3 indicators, and estimates the fit and reliability of the model.
The correlation analysis between the impedance loss coefficient delta value of the suitable peak frequency point determined by the electrical impedance spectrum and the obtained soluble sugar content is carried out, and the result is shown in table 2, and table 2 shows the correlation between the soluble sugar content of the kalopanax septemlobus stems and the impedance loss coefficient delta during the cold-resistant exercise period of the invention.
TABLE 2 correlation of soluble sugar content of kalopanax pohuashanensis stems during cold-resistant exercise according to the invention with EIS parameter delta
| Coefficient of impedance loss delta | Coefficient of correlation r |
| 12000Hz | -0.957* |
| 15000Hz | -0.914* |
Note: the number of stem samples n is 75; indicates significance at the 0.05 level.
As can be seen from Table 2, the resistance loss coefficient delta of the kalopanax septemlobus stem has better correlation with the content of soluble sugar during the cold-resistant exercise, and the correlation coefficient reaches-0.957 and-0.914;
modeling is carried out by 50 groups of samples, and the resistance loss coefficient delta value of the sorbus pohuashanensis stem is better related to soluble sugar at 12000Hz, so that the method for estimating the content of the soluble sugar in the sorbus pohuashanensis stem by using the frequency point has higher feasibility. Thus, linear and nonlinear regression analyses were performed using the 12000Hz delta value of the stem as the independent variable and the soluble sugars as dependent variables, while simultaneously determining the maximum R2The preferred principle selects the best estimation model. As shown in Table 3, Table 3 shows the regression model of the soluble sugar and the delta resistance loss coefficient of Liriodendron bungei during cold-resistant exercise according to the present invention.
TABLE 3 regression model of soluble sugar and delta values of impedance loss coefficient for kalopanax septemlobus stem during cold-resistant exercise according to the present invention
Note: the number of samples of stems n is 50.
The best estimation model of the stem among the obtained estimation models is yStem of a tree＝-18.67δ3-20.47δ27.697 δ -0.790, coefficient of determination 0.942.
Example 3
Taking samples to be tested of 25 groups of kalopanax pohuashanensis stems, determining the impedance loss coefficient delta value according to the method in the embodiment 2, and calculating the content of soluble sugar in the samples to be tested of 25 groups of stems according to the regression model of the stems obtained in the embodiment 2;
and determining the content of soluble sugar in the sample to be detected of the 25 groups of stems by adopting an anthrone chromogenic method, and using the content to detect the model precision. The results are shown in table 4, and table 4 shows the fitting evaluation index of the measured value and the predicted value of the soluble sugar content of the kalopanax septemlobus stems obtained by the present invention.
TABLE 4 fitting evaluation index of measured and predicted values of soluble sugar content in kalopanax septemlobus stems obtained by the present invention
As can be seen from Table 4, the estimation models established during the cold-resistant exercise by the method provided by the invention through the impedance loss coefficient delta and the soluble sugar content change all obtain better verification results, and the three model determination coefficients (R) of the stem2) 0.834, 0.801, and 0.846 are achieved, respectively, but the model with y ═ 0.447x +0.038 has a small root mean square error (RWSE ═ 3.47) and a small relative error (RE ═ 9.84%), and the prediction accuracy reaches 90.06%.
Example 4
The sorbus pohuashanensis stem was frozen at the freezing temperature set in table 1, and the soluble sugar content in the sorbus pohuashanensis stem was calculated according to the regression model obtained in example 4 after freezing.
As shown in FIG. 2, it can be seen from FIG. 2 that the soluble sugar content of the kalopanax septemlobus stem increases during the cold-resistant exercise period, and that the soluble sugar content increases 98.47% at the end of the cold-resistant exercise period as compared with the initial period.
From the above embodiments, the present invention provides a method for measuring the content of soluble sugar in catalpa bungei stems by electrical impedance, which comprises the following steps: detecting an electrical impedance spectrum, a resistance value and a capacitive reactance value of a to-be-detected sample of the kalopanax septemlobus; determining a peak value suitable for calculating an impedance loss coefficient delta according to the electrical impedance map of the sample to be detected; calculating an impedance loss coefficient delta formed by a resistance value and a capacitive reactance value according to the proper peak value; and establishing a regression model of the resistance loss coefficient delta value and the soluble sugar content of the sorbus pohuashanensis, and obtaining the soluble sugar content of the sorbus pohuashanensis according to the regression model and the resistance loss coefficient delta value of the sample to be detected. The method provided by the invention establishes a regression model between the electrical impedance parameter of the kalopanax septemlobus stem and the soluble sugar content thereof, so that the soluble sugar content in the kalopanax septemlobus stem is calculated according to the measured electrical impedance parameter, and the rapid and real-time diagnosis of the soluble sugar content can be realized. Moreover, the invention also establishes the relation between the electrical impedance parameter and the cold resistance change, thereby being capable of knowing the cold resistance of the kalopanax pohuashanensis stem according to the change of the electrical impedance parameter and providing important theoretical basis and technical support for the estimation of the cold resistance change.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (4)
1. A method for measuring the content of soluble sugar in kalopanax pohuashanensis by an electrical impedance loss coefficient delta value method comprises the following steps:
detecting the resistance value and the volume resistance value of a sample to be detected of the kalopanax pohuashanensis, and drawing an electrical impedance map;
determining a peak value suitable for calculating an impedance loss coefficient delta according to the electrical impedance map of the sample to be detected;
calculating an impedance loss coefficient delta value formed by a resistance value and a capacitive reactance value according to the peak value;
establishing a regression model of the resistance loss coefficient delta value and the soluble sugar content of the sorbus pohuashanensis, and obtaining the soluble sugar content of the sorbus pohuashanensis according to the regression model and the resistance loss coefficient delta value of the sample to be detected;
the regression model of the resistance loss coefficient delta value and the soluble sugar content of the kalopanax pohuashanensis stem is a linear equation, a quadratic equation or a cubic equation;
when the regression model of the resistance loss coefficient delta value and the soluble sugar content of the kalopanax pohuashanensis stem is a linear equation, the linear equation is as follows: y is-0.447 delta +0.038, wherein delta is the impedance loss coefficient delta value of the kalopanax pohuashanensis stem, and y is the soluble sugar content;
when the regression model of the resistance loss coefficient delta value and the soluble sugar content of the kalopanax pohuashanensis stem is a quadratic equation, the quadratic equation is as follows: y is-0.017 delta2-0.460 δ +0.036, where δ is the resistance loss coefficient δ value for the kalopanax pohuashanensis stem and y is the soluble sugar content;
when the regression model of the resistance loss coefficient delta value and the soluble sugar content of the kalopanax pohuashanensis stem is a cubic equation, the cubic equation is as follows: y is-18.67 delta3-20.47δ27.697 delta-0.790, where delta is the impedance loss coefficient delta value of the kalopanax pohuashanensis stem and y is the soluble sugar content.
2. The method of claim 1, wherein the peak is at 12KHz or 15 KHz.
3. The method according to claim 1, wherein the regression model of the ash resistance loss coefficient δ value and the soluble sugar content comprises the ash resistance loss coefficient δ value as an independent variable and the soluble sugar content as a dependent variable.
4. The method according to claim 1, wherein the sample to be tested is a stem of kalopanax septemlobus.
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