CN108107024A - A kind of intelligence PCR instrument - Google Patents
A kind of intelligence PCR instrument Download PDFInfo
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- CN108107024A CN108107024A CN201611054406.3A CN201611054406A CN108107024A CN 108107024 A CN108107024 A CN 108107024A CN 201611054406 A CN201611054406 A CN 201611054406A CN 108107024 A CN108107024 A CN 108107024A
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- module
- pcr instrument
- electrically connected
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- intelligence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G08—SIGNALLING
- G08C—TRANSMISSION SYSTEMS FOR MEASURED VALUES, CONTROL OR SIMILAR SIGNALS
- G08C17/00—Arrangements for transmitting signals characterised by the use of a wireless electrical link
- G08C17/02—Arrangements for transmitting signals characterised by the use of a wireless electrical link using a radio link
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- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
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- Computer Networks & Wireless Communication (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of intelligent PCR instrument, including:Housing;Thermocirculator is arranged in housing, for carrying out temperature conditionss to PCR process;Fluorescence detection device is arranged in housing, for carrying out fluoroscopic examination to PCR result;Master board is arranged in housing, is electrically connected with thermocirculator and fluorescence detection device;Multiple interfaces, are arranged on housing, for connecting exterior terminal and being communicated with exterior terminal;Interface convertor is arranged in master board, is electrically connected with multiple interfaces, for the data of master board to be converted into the information that any of multiple interfaces interface can transmit;Wireless communication module is arranged in master board, for realizing wireless telecommunications with exterior terminal.By the present invention, staff can be connected using various types of terminals with PCR instrument, anywhere realize the monitoring to PCR processes, greatly improve convenience and the intelligence of PCR instrument.
Description
Technical field
The present invention relates to biochemical equipment fields, and in particular to a kind of intelligence PCR instrument.
Background technology
PCR (Polymerase Chain Reaction, referred to as PCR) is a kind of for amplification amplification
The Protocols in Molecular Biology of specific DNA fragmentation, it is considered as the special DNA replication dna of in vitro, the maximum feature of PCR,
It is that micro DNA can be significantly increased.The equipment for being presently used for carrying out PCR is called PCR instrument.At present, market
On PCR instrument species it is various, be broadly divided into common PCR instrument, grads PCR instrument, In situPCR instrument, real-time fluorescence quantitative PCR instrument
Deng.
PCR is by being denatured -- annealing --, and three fundamental reaction steps of extension are formed, and PCR instrument is exactly by the way that sample is controlled to reach not
It is synthermal, the DNA fragmentation being amplified is denatured, anneal and polymerization processing, expanded at double with the amount reached DNA fragmentation
Purpose.Since the reaction process of PCR takes longer, staff, which can only be sitting in, to be waited beside dedicated computer and closes at any time
Reaction process is noted, and other affairs can not be handled, relevant affairs of especially going out.
The content of the invention
The technical problem to be solved in the present invention is existing PCR instrument when being reacted, and staff is needed special
Computer beside wait, other affairs can not be handled, especially go out relevant affairs, so as to provide a kind of intelligence PCR
Instrument.
An aspect of of the present present invention provides a kind of intelligent PCR instrument, including:Housing;Thermocirculator is arranged on described
In housing, for carrying out temperature conditionss to PCR process;Fluorescence detection device is arranged in the housing, is used
In to the progress fluoroscopic examination of PCR result;Master board is arranged in the housing, with the thermocirculator
It is electrically connected with the fluorescence detection device;Multiple interfaces, set on the housing, for connect exterior terminal and with institute
It states exterior terminal to be communicated, the multiple interface includes following at least two:RJ-45 interfaces, RJ-11 interface, SC optical fiber connect
Mouth, fddi interface, AUI interfaces, bnc interface, Console interfaces, USB interface;Interface convertor is arranged on the master board
On, it is electrically connected with the multiple interface, it is any in the multiple interface for the data of the master board to be converted into
The information that a interface can transmit;Wireless communication module is arranged in the master board, for realizing nothing with exterior terminal
Line communicates.
Optionally, the wireless communication module includes at least one of:WIFI module, bluetooth module, Zigbee module,
Mobile communication module.
Optionally, the mobile communication module includes:Gsm module, GPRS module, 3G module, 4G modules.
Optionally, the exterior terminal is one of the following:Router, desktop computer, mobile phone, tablet computer, notebook electricity
Brain.
Optionally, the thermocirculator includes:Orifice plate, for placing sample tube;Heating module is arranged on the hole
Below plate, it is connected with the master board, for being heated or being cooled down;Hot cover is arranged on the sample tube,
It is contacted with the sample tube mouth.
Optionally, further include:Sub-control plate is connected with the hot cover, for controlling the hot cover.
Optionally, further include:Master controller is arranged in the master board, and the master controller passes through the master control
Interface in making sheet is electrically connected with the thermocirculator and the fluorescence detection device, for controlling polymerase chain reaction
The process answered;Submaster controller is arranged in the master board, with the master controller, the wireless communication module and institute
It states multiple interfaces to be electrically connected, for handling the communication with exterior terminal.
Optionally, further include:Power detecting module is electrically connected with the master controller, for detecting power supply unit output
Electrical energy parameter;Shine detection module, is electrically connected with the master controller, for detecting the luminous ginseng of the fluorescence detection device
Number;Temperature sensor is electrically connected with the master controller, for detecting the temperature of the heating module;Hot lid temperature sensor,
It is electrically connected with the master controller, for detecting the temperature of the hot cover.
Optionally, the power detecting module, the luminous detection module, the temperature sensor and the hot lid temperature
Sensor is electrically connected respectively with the submaster controller, and the submaster controller is used for the power detecting module, the luminous inspection
Survey module, the testing result of the temperature sensor and the hot lid temperature sensor by the wireless communication module and/or
The multiple interface is sent to exterior terminal.
In the embodiment of the present invention, multiple interfaces are integrated in PCR instrument, can there is multiple and different species, the interface of signal, with
Suitable for various exterior terminal interfaces, in this way, staff can be connected using various types of terminals with PCR instrument, without making
With dedicated computer, you can it is monitored, also, by adding wireless communication module, can realize wireless monitor,
As long as exterior terminal establishes wireless connection with PCR instrument, staff can anywhere realize the monitoring to PCR processes,
Greatly improve convenience and the intelligence of PCR instrument.
Description of the drawings
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution of the prior art
Embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, in describing below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the schematic diagram of a specific example of intelligent PCR instrument in the embodiment of the present invention;
Fig. 2 is the schematic diagram of another specific example of intelligent PCR instrument in the embodiment of the present invention.
Specific embodiment
Technical scheme is clearly and completely described below in conjunction with attached drawing, it is clear that described implementation
Example is part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill
Personnel's all other embodiments obtained without making creative work, belong to the scope of protection of the invention.
In the description of the present invention, it is necessary to explanation, term " " center ", " on ", " under ", "left", "right", " vertical ",
The orientation or position relationship of the instructions such as " level ", " interior ", " outer " be based on orientation shown in the drawings or position relationship, merely to
Convenient for the description present invention and simplify description rather than instruction or imply signified device or element must have specific orientation,
With specific azimuth configuration and operation, therefore it is not considered as limiting the invention.In addition, term " first ", " second ",
" the 3rd " is only used for description purpose, and it is not intended that instruction or hint relative importance.
In the description of the present invention, it is necessary to illustrate, unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected or be integrally connected;It can
To be mechanical connection or be electrically connected;It can be directly connected, can also be indirectly connected by intermediary, it can be with
It is the connection inside two elements, can is wireless connection or wired connection.For those of ordinary skill in the art
For, the concrete meaning of above-mentioned term in the present invention can be understood with concrete condition.
As long as in addition, technical characteristic involved in invention described below different embodiments non-structure each other
It can be combined with each other into conflict.
The present embodiment provides a kind of intelligent PCR instrument, as shown in Figure 1, the intelligence PCR instrument includes:Housing 10, thermal cycle
Device 20, fluorescence detection device 30, master board 40, multiple interfaces 50, interface convertor 60 and wireless communication module 70.
Thermocirculator 20 is arranged in housing 10, for carrying out temperature conditionss to PCR process;Fluorescence
Detection device 30 is arranged in housing 10, for carrying out fluoroscopic examination to PCR result;Master board 40 is set
In housing 10, it is electrically connected with thermocirculator 20 and fluorescence detection device 30;Multiple interfaces 50 are set on the housing 10,
For connecting exterior terminal and being communicated with exterior terminal, multiple interfaces 50 include following at least two:RJ-45 interfaces, RJ-
11 interfaces, SC optical fiber interfaces, fddi interface, AUI interfaces, bnc interface, Console interfaces, USB interface;Interface convertor 60 is set
It puts in master board 40, is electrically connected with multiple interfaces 50, for the data of master board to be converted into multiple interfaces 50
Any of the information that can transmit of interface;Wireless communication module 70 is arranged in master board 40, is used for and exterior terminal
Realize wireless telecommunications.
In the embodiment of the present invention, multiple interfaces are integrated in PCR instrument, can there is multiple and different species, the interface of signal, with
Suitable for various exterior terminal interfaces, in this way, staff can be connected using various types of terminals with PCR instrument, without making
With dedicated computer, you can it is monitored, also, by adding wireless communication module, can realize wireless monitor,
As long as exterior terminal establishes wireless connection with PCR instrument, staff can anywhere realize the monitoring to PCR processes,
Greatly improve convenience and the intelligence of PCR instrument.
Optionally, the wireless communication module of the embodiment of the present invention includes at least one of:WIFI module, bluetooth module,
Zigbee module, mobile communication module.For wireless monitor closely, exterior terminal can with PCR instrument by bluetooth module,
Zigbee module, WIFI module establish connection, realize wireless telecommunications and data monitoring, for remote monitoring, then can use and move
Dynamic communication module is established long distance wireless with exterior terminal and is connected, and facilitates the reaction process and knot of staff's remote monitoring PCR instrument
Fruit.
Further, mobile communication module includes:Gsm module, GPRS module, 3G module, 4G modules.
In the embodiment of the present invention, exterior terminal is one of the following:Router, desktop computer, mobile phone, tablet computer, notes
This computer.Wherein, when exterior terminal is router, it that is to say and the reaction process data and result of PCR instrument are uploaded to network
Server, staff can extract these data on network server.In the embodiment of the present invention, user can use individual
The terminals such as mobile phone, computer and PCR instrument, which are established, to be connected, so as to the monitoring to showing anywhere in fact.
In the embodiment of the present invention, thermocirculator is the main place of PCR reactions, including:Orifice plate, for placing sample
Test tube;Heating module is arranged below orifice plate, is connected with master board, for being heated or being cooled down;Hot cover, if
It puts on sample tube, is contacted with sample tube mouth.
-- annealing -- is denatured to sample tube on orifice plate and extends three elementary processes, then by fluoroscopic examination
Device realizes fluoroscopic examination, draws testing result.Further, intelligent PCR instrument further includes:Sub-control plate connects with hot cover
It connects, for controlling hot cover.Specifically, also sub-control plate both for controlling the temperature of hot cover, is additionally operable to control
Heat the movement of cover.
Optionally, as shown in Fig. 2, the intelligent PCR instrument of the embodiment of the present invention further includes:Master controller 401, is arranged on master
On control panel 40, master controller 401 is divided by the interface in master board 40 and thermocirculator 20 and fluorescence detection device 30
It is not electrically connected, for controlling the process of PCR;Submaster controller 402 is arranged in master board 40, with main control
Device 401, wireless communication module 70 and multiple interfaces 50 are electrically connected, for handling the communication with exterior terminal.
In the embodiment of the present invention, the process of PCR is controlled by master controller 401, for example, temperature controls, is glimmering
Light detection control etc., by submaster controller 402 realize with the data communication before exterior terminal, can so reduce master controller
401 load avoids the problem that breaking down to block since 401 load of master controller is excessive stopping.In addition, it is carried out using dual controller
Control, it is more efficient without using high performance single processor.
Optionally, the intelligent PCR instrument of the embodiment of the present invention further includes:Power detecting module, is electrically connected with master controller,
For detecting the electrical energy parameter of power supply unit output;Shine detection module, is electrically connected with master controller, for detecting fluoroscopic examination
The luminous parameters of device;Temperature sensor is electrically connected with master controller, for detecting the temperature of heating module;Hot lid temperature passes
Sensor is electrically connected with master controller, for detecting the temperature of hot cover.
In the present embodiment, the electrical energy parameter of power detecting module detection power supply unit output can reflect power supply unit
Whether exception is occurred.Similarly, it is different that the luminous parameters that luminous detection module detects can reflect whether fluorescence detection device occurs
Often, specifically whether the luminescent system of reaction fluorescence detection device there is exception;The temperature parameter that temperature sensor detects can
Whether reflection heating module there is exception;The heating for the hot cover that the temperature parameter of hot lid temperature sensor detection can reflect
It is whether abnormal.
According to embodiments of the present invention, the working condition of PCR instrument is detected by setting detection module or sensor, realized
Self-test to PCR instrument so as to determine the component being abnormal in time, avoids utilizing the component sustained response being abnormal
Or the problem of experiment reaction time caused by experiment extends, efficiency is low.
Further, the power detecting module of the embodiment of the present invention, luminous detection module, temperature sensor and Re Gai temperature
Sensor is electrically connected respectively with submaster controller, and submaster controller is used for power detecting module, shine detection module, temperature sensor
Exterior terminal is sent to by wireless communication module and/or multiple interfaces with the testing result of hot lid temperature sensor.
In the embodiment of the present invention, the parameter that PCR instrument is carried out to self-test by submaster controller is sent to exterior terminal, so that
It can be the working condition that may know that PCR instrument by exterior terminal to obtain user, in order to react in time, adjust work.
Obviously, the above embodiments are merely examples for clarifying the description, and is not intended to limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And the obvious variation thus extended out or
Among changing still in the protection domain of the application.
Claims (9)
1. a kind of intelligence PCR instrument, which is characterized in that including:
Housing;
Thermocirculator is arranged in the housing, for carrying out temperature conditionss to PCR process;
Fluorescence detection device is arranged in the housing, for carrying out fluoroscopic examination to PCR result;
Master board is arranged in the housing, is electrically connected with the thermocirculator and the fluorescence detection device;
Multiple interfaces are set on the housing, described more for connecting exterior terminal and being communicated with the exterior terminal
A interface includes following at least two:RJ-45 interfaces, RJ-11 interface, SC optical fiber interfaces, fddi interface, AUI interfaces, BNC connect
Mouth, Console interfaces, USB interface;
Interface convertor is arranged in the master board, is electrically connected with the multiple interface, for by the main control
The data of plate are converted into the information that any of the multiple interface interface can transmit;
Wireless communication module is arranged in the master board, for realizing wireless telecommunications with exterior terminal.
2. it is according to claim 1 intelligence PCR instrument, which is characterized in that the wireless communication module include it is following at least
One of:WIFI module, bluetooth module, Z igbee modules, mobile communication module.
3. intelligence PCR instrument according to claim 2, which is characterized in that the mobile communication module includes:Gsm module,
GPRS module, 3G module, 4G modules.
4. intelligence PCR instrument according to claim 1, which is characterized in that the exterior terminal is one of the following:Routing
Device, desktop computer, mobile phone, tablet computer, laptop.
5. intelligence PCR instrument according to claim 1, which is characterized in that the thermocirculator includes:
Orifice plate, for placing sample tube;
Heating module is arranged below the orifice plate, is connected with the master board, for being heated or being cooled down;
Hot cover is arranged on the sample tube, is contacted with the sample tube mouth.
6. intelligence PCR instrument according to claim 5, which is characterized in that further include:
Sub-control plate is connected with the hot cover, for controlling the hot cover.
7. intelligence PCR instrument according to claim 6, which is characterized in that further include:
Master controller is arranged in the master board, the master controller by the interface in the master board with it is described
Thermocirculator and the fluorescence detection device are electrically connected, for controlling the process of PCR;
Submaster controller is arranged in the master board, with the master controller, the wireless communication module and the multiple
Interface is electrically connected, for handling the communication with exterior terminal.
8. intelligence PCR instrument according to claim 7, which is characterized in that further include:
Power detecting module is electrically connected with the master controller, for detecting the electrical energy parameter of power supply unit output;
Shine detection module, is electrically connected with the master controller, for detecting the luminous parameters of the fluorescence detection device;
Temperature sensor is electrically connected with the master controller, for detecting the temperature of the heating module;
Hot lid temperature sensor, is electrically connected with the master controller, for detecting the temperature of the hot cover.
9. intelligence PCR instrument according to claim 8, which is characterized in that the power detecting module, the luminous detection
Module, the temperature sensor and the hot lid temperature sensor are electrically connected respectively with the submaster controller, the submaster controller
For by the power detecting module, the luminous detection module, the temperature sensor and the hot lid temperature sensor
Testing result is sent to exterior terminal by the wireless communication module and/or the multiple interface.
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CN201611054406.3A CN108107024A (en) | 2016-11-25 | 2016-11-25 | A kind of intelligence PCR instrument |
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CN201611054406.3A CN108107024A (en) | 2016-11-25 | 2016-11-25 | A kind of intelligence PCR instrument |
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CN108107024A true CN108107024A (en) | 2018-06-01 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109759162A (en) * | 2019-01-15 | 2019-05-17 | 北京市计量检测科学研究院 | Gene-amplificative instrament test stone device |
CN111139179A (en) * | 2019-12-26 | 2020-05-12 | 中国计量大学 | Portable isothermal amplification rapid detector capable of independently controlling temperature |
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