CN108106922A - A kind of concentration mixing mechanism - Google Patents
A kind of concentration mixing mechanism Download PDFInfo
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- CN108106922A CN108106922A CN201810094206.3A CN201810094206A CN108106922A CN 108106922 A CN108106922 A CN 108106922A CN 201810094206 A CN201810094206 A CN 201810094206A CN 108106922 A CN108106922 A CN 108106922A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The invention discloses a kind of concentration mixing mechanism, including：Specimen bottle, including bottle and with the matched bottle cap of the bottle, filter device is provided in the bottle, the bottle, the bottle cap and institute's filter device form the first cavity, and the bottom of bottle and institute's filter device of the bottle form the second cavity；First lifting assembly can drive concentration pin to puncture bottom of bottle；Pumping components are able to carry out pumping operation；Air blowing component is able to carry out reversed air blowing operation；Commutate component, can realize the switching of the conducting of the pumping components and the concentration pin and the conducting of the air blowing component and the concentration pin；And controller, the controller controls the first lifting assembly to drive the direction of concentration pin to the close specimen bottle mobile and punctures bottom of bottle into second cavity, and commutation switch between components pumping components and air blowing component is controlled to perform pumping operation and reversed air blowing operation repeatedly.
Description
Technical field
The present invention relates to medical biological assay technical field, more specifically to a kind of concentration mixing mechanism.
Background technology
In medical test, it is often necessary to be enriched with certain specific cell in sample, then carry out cell again
Identification, in order to ensure the accuracy examined and required cell quantity during inspection, it usually needs in the condition of sterile sealing
Under be enriched with, can so prevent from infecting and polluting, ensure the safety of operating personnel and ambient enviroment.
At present, liquid-based product uses two methods for cell enrichment in the market：One kind centrifuges after mixing for concussion
Enrichment of cell.Wherein operating process is：Manually sample, fully shaking is uniform in whirlpool oscillator, then will manually mix equal
Sample after even, which is placed, to be transferred samples in sample transfer in centrifuge tube, then by centrifuge tube be put into centrifuge to sample from
The heart is taken out supernatant with negative pressure after centrifugation, is then centrifuged again, last Exfoliative cells etc. diagnosis composition aggregation be attached on from
Heart bottom of the tube forms cell mass, then outwells liquid above cell mass (containing ingredients such as red blood cell, mucus in liquid), reaches
It except the purpose of interference component enrichment of cell, then will be shaken on centrifuge tube earthquake device, make that the epithelial cell of collection is fully dispersed to be
Individual cells finally shift sectioning cells.It is more to have suffered operating process manual intervention, operation is cumbersome, and elapsed time is long.
Another kind is membrane type negative pressure drainage enrichment transfer cell.Wherein operating process is：Manually in specimen fluids bottle
In rinse sample brush after sample brush is abandoned, there are cell loss risks for the process.Again by machine on specimen fluids bottle, using lower end band
Have in the tubular filter cylinder insertion specimen fluids bottle of a tunic, cartridge filter upper end is ined succession negative pressure pump, is less than on film there are many diameter upper
Chrotoplast, more than mucus little particle, the hole of leucocyte first by rotating through lauter tub, drives liquid to rotate, utilizes liquid turn
The shearing force of formation disperses mucus, mixing cell.It is moved up and down, made by negative-pressure ward, cartridge filter after mixing with cells is uniform
Chrotoplast is adsorbed on film, and mucus, leucocyte etc. is made to reach deimpurity purpose by film, then filter vat is reversed, filter membrane
With slide contact, the cell stayed on film is allowed to be adsorbed by positive pressure transfer on glass slide.It is by then passing through negative pressure that cell is straight
Absorption is connect on film, cell non-uniform can be distributed in film surface, be directly born against by the cell on film on slide, and film-making exists
Cell overlap risk.Though this method eliminates substantial amounts of manual operations, the overlapping of cell and loss are uncontrollable.
Therefore, the quality of cell enrichment how is improved, becomes the technical issues of those skilled in the art are urgently to be resolved hurrily.
The content of the invention
In view of this, the technical problems to be solved by the invention are how to improve the quality of cell enrichment, for this purpose, of the invention
Provide a kind of concentration mixing mechanism.
To achieve the above object, the present invention provides following technical solution：
A kind of concentration mixing mechanism, including：
Specimen bottle, including bottle and with the matched bottle cap of the bottle, be provided with filter device in the bottle, it is described
Bottle, the bottle cap and institute's filter device form the first cavity, and the bottom of bottle and institute's filter device of the bottle form the second cavity；
First lifting assembly can drive concentration pin to puncture bottom of bottle；
Pumping components are able to carry out pumping operation；
Air blowing component is able to carry out reversed air blowing operation；
Commutate component, can realize the pumping components with it is described concentrate pin conducting and the air blowing component with it is described dense
The switching of the conducting of shortening；And
Controller, the controller control the first lifting assembly that concentration pin is driven to be moved to close to the direction of the specimen bottle
And bottom of bottle is punctured into second cavity, and commutation switch between components pumping components and air blowing component is controlled to perform suction repeatedly and make
Industry and reversed air blowing operation.
Preferably, in above-mentioned concentration mixing mechanism, the position-limit mechanism for limiting the bottle cap bounce is further included.
Preferably, in above-mentioned concentration mixing mechanism, the concentration position of the position-limit mechanism is provided with rotatable limited block, institute
Stating limited block can abut against with the bottle cap.
Preferably, in above-mentioned concentration mixing mechanism, load maintainer is further included, the load maintainer includes sample disk, described
It is provided with to place the loading hole of specimen bottle on sample disk.
Preferably, in above-mentioned concentration mixing mechanism, it is described commutation component be reversal valve when, it is described concentration pin by pipeline with
The outlet of the reversal valve, the first inlet communication of the pumping components and the reversal valve, the air blowing component and institute
State the second inlet communication of reversal valve, when the reversal valve is located at first state, the first import of the reversal valve with it is described
The outlet conducting of reversal valve, the second import and the outlet of the reversal valve of the reversal valve are non-conduction；When the reversal valve position
When the second state, the first import and the outlet of the reversal valve of the reversal valve are non-conduction, the reversal valve second into
Mouth is turned on the outlet of the reversal valve.
Preferably, in above-mentioned concentration mixing mechanism, further include sample charging mechanism, the sample charging mechanism can puncture bottle cap and to
Cell Buffer is added in first cavity；
The concentration mixing mechanism can also perform mixing operation, after Cell Buffer is added in first cavity,
The controller control concentration mixing mechanism performs mixing operation.
Preferably, in above-mentioned concentration mixing mechanism, the sample charging mechanism includes plunger pump, solenoid valve and sample needle, described
Sample needle is connected by pipeline with the first outlet of the solenoid valve, and second outlet and the Cell Buffer of the solenoid valve connect
Logical, the import of the solenoid valve is connected with the plunger pump；
When the solenoid valve is located at the third state, the import of the first outlet of the solenoid valve and the solenoid valve is non-to be led
Logical, the second outlet of the solenoid valve is turned on the import of the solenoid valve；
When the solenoid valve is located at four states, the first outlet of the solenoid valve and the import of the solenoid valve are led
Logical, the second outlet of the solenoid valve and the import of the solenoid valve are non-conduction.
Preferably, in above-mentioned concentration mixing mechanism, the sample charging mechanism, which further includes, pushes away sample component, described to push away sample Component driver
The sample needle punctures bottle cap.
Preferably, in above-mentioned concentration mixing mechanism, the concentration mixing mechanism further includes：
Rotary components are able to carry out mixing operation；And
Second lifting assembly, can drive rotary components to it is mobile close to the direction of the specimen bottle and with the bottom of bottle pair
It connects；
After Cell Buffer is added in the first cavity, the controller controls the second lifting assembly operation, and controls
Make execution mixing operation after the rotary components are docked with the bottom of bottle.
When as can be seen from the above scheme, using the concentration mixing mechanism of the present invention, controller controls the first lifting assembly
Operation, concentration pin are moved to close to the direction of specimen bottle, until concentration pin punctures bottom of bottle, concentration pin is stretched in the second cavity；
Controller control and suck component, air blowing component and commutation assembly operating, when carrying out pumping operation, commutation element turns suction
Component and concentration pin, when carrying out reversed air blowing operation, commutation element turns air blowing component and concentration pin.Controller passes through control
Commutating for component of commutating realizes the switching of above two conduction status, makees so as to fulfill pumping operation and reversely air blowing is performed repeatedly
Industry.
Since concentration mixing mechanism using the present invention is dense by performing pumping operation and reversed air blowing operation progress cell
Contracting blocks the filter device of specimen bottle and concentration pin therefore, it is possible to be effectively prevented, can while cell concentration is accelerated
Cell superposition is efficiently reduced, so as to improve the quality of cell enrichment.
Description of the drawings
It in order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention, for those of ordinary skill in the art, without creative efforts, can be with
Other attached drawings are obtained according to these attached drawings.
The schematic cross-sectional view for the specimen bottle that Fig. 1 is provided by the embodiment of the present invention；
The dimensional structure diagram for the specimen bottle that Fig. 2 is provided by the embodiment of the present invention；
The dimensional structure diagram for the load maintainer that Fig. 3 is provided by the embodiment of the present invention；
The schematic cross-sectional view for the position-limit mechanism that Fig. 4 is provided by the embodiment of the present invention；
The solid of the cooperation of concentration mixing mechanism, load maintainer and position-limit mechanism that Fig. 5 is provided by the embodiment of the present invention
Structure diagram；
Fig. 6 is illustrated by the stereochemical structure of the cooperation of concentration mixing mechanism and position-limit mechanism that the embodiment of the present invention provides
Figure；
The concentration mixing mechanism partial structurtes block diagram that Fig. 7 is provided by the embodiment of the present invention；
A kind of structure diagram for sample charging mechanism that Fig. 8 is provided by the embodiment of the present invention.
Wherein,
100 it is specimen bottle, 101 be bottle, 102 be bottle cap, 103 be bottom of bottle, 104 be filter device, 105 is the first chamber
Body, 106 be the second cavity, 108 be reinforcing plate, 1011 be the second extension, 1021 be the first easy puncture portion, 1031 be first to prolong
Extending portion, 1032 are the second easy puncture portion；
200 it is load maintainer, 201 be sample disk, 2011 is loading hole；
300 it is position-limit mechanism, 301 be support plate, 302 be limiting plate, 303 be limited block, 304 be bearing, 305 is sample-adding
Hole；
500 it is concentration mixing mechanism, 501 be the first lifting assembly, 502 be pumping components, 503 be air blowing component, 504 is
Commutation component, 505 for the second lifting assembly, 506 be rotary components, 5011 be first straight line guide rail, 5012 be the first sliding block,
5013 it is first straight line motor, 5014 be concentration pin, 5051 be second straight line guide rail, 5052 be the second sliding block, 5053 is second
Linear motor, 5061 be rotation shift fork, 5062 be transmission component；
600 it is sample charging mechanism, 601 be plunger pump, 602 be solenoid valve, 603 is sample needle.
Specific embodiment
The core of the present invention is to provide a kind of matter for concentrating mixing mechanism and cell concentration method, improving cell enrichment
Amount.
In addition, embodiments illustrated below does not play any restriction effect to the content of the invention recorded in claim.In addition,
The full content of composition represented by example below is not limited to must as the solution of the invention recorded in claim
It needs.
It please refers to Fig.1 to Fig. 8, the concentration mixing mechanism of the embodiment of the present invention, including：
Specimen bottle 100, including bottle 101 and with 101 matched bottle cap 102 of bottle, filtering dress is provided in bottle 101
Put 104, bottle 101, bottle cap 102 and institute's filter device 104 form the first cavity 105, the bottom of bottle 103 of bottle 101 and are filtered
Device 104 forms the second cavity 106；
First lifting assembly 501 can drive concentration pin 5014 to puncture bottom of bottle 103；
Pumping components 502, are able to carry out pumping operation；
Air blowing component 503 is able to carry out reversed air blowing operation；
Commutate component 504, can realize pumping components 502 with concentrate pin 5014 conducting and air blowing component 503 with concentration
The switching of the conducting of pin 5014；And
Controller, controller control the first lifting assembly 501 that concentration pin 5014 is driven to be moved to close to the direction of specimen bottle 100
It moves and punctures bottom of bottle 103 and enter the second cavity 105, and commutation component 504 is controlled to switch pumping components 502 and air blowing component 503
Pumping operation and reversed air blowing operation are performed repeatedly.
Controller controls the first lifting assembly 501 to run, and concentration pin 5014 is moved to close to the direction of specimen bottle 100, directly
Bottom of bottle 103 is punctured to concentration pin 5014, concentration pin 5014 is stretched in the second cavity 106；Controller control and suck component 502,
Air blowing component 503 and commutation component 504 run, when carrying out pumping operation, commutation component 504 turn on pumping components 502 and
Pin 5014 is concentrated, when carrying out reversed air blowing operation, commutation component 504 turns on air blowing component 503 and concentration pin 5014.Controller
The switching of above two conduction status is realized by the commutation for controlling commutation component 504, so as to fulfill pumping operation is performed repeatedly
With reversed air blowing operation.
Since concentration mixing mechanism using the present invention is dense by performing pumping operation and reversed air blowing operation progress cell
Contracting therefore, it is possible to be effectively prevented the filter device 104 for blocking specimen bottle, while cell concentration is accelerated, can effectively subtract
Few cell superposition, so as to improve the quality of cell enrichment.
It should be noted that pumping operation is that the invalid liquid in the second cavity 106 is detached specimen bottle by aspirating
Outside 100；Reversed air blowing operation is blown into the second cavity 106, the filtering direction of blowing direction and filter device 104 on the contrary,
The cell blocked on filter device 104 can be blown afloat during air blowing, to achieve the purpose that dredge filter device 104, in addition, blowing afloat
When the invalid liquid concentrated in pin 5014 can also be blown back to the second cavity 106, can also reach dredging concentration pin 5014
Purpose.
It please refers to Fig.1 and Fig. 2, sample is uniformly mixed with cell-preservation liquid in the first cavity 105 in the embodiment of the present invention
Afterwards, invalid penetration by liquid filter device 104 is filled into the second cavity 106, and effective cell is retained in the first cavity 105, from
And achieve the purpose that cell concentration.
In order to optimize said program, bottle cap 102 is provided with the first easy puncture portion 1021, and the first easy puncture portion 1021 is easily quilt
The material manufacture of puncture forms.When the sample in specimen bottle 100 and cell-preservation liquid after mixing, make in filter device 104
Under, mixed liquor is filtered, so as to achieve the purpose that cell concentration, since bottle cap 102 is provided with the first easy puncture portion
1021, Cell Buffer can be injected into the first cavity 105 by puncturing, without opening bottle cap 102, so as to reduce sample
Sample in bottle 100 in first cavity 105 is by the risk of air pollution.
Similarly, in the embodiment of the present invention, the bottom of bottle 103 of bottle 101 is provided with the second easy puncture portion 1032, is filled by filtering
The 104 invalid liquid being filled into the second cavity 106 are put, invalid liquid are taken out by being punctured in the second cavity 106, so as to drop
Second cavity 106 is by the risk of air pollution in low specimen bottle 100.
The bottom of bottle 103 of bottle 101 is planar structure or is up big and down small funnel-shaped structure.Using funnel-shaped structure
When, the size in the second easy puncture portion 1032 can be reduced, achievees the purpose that save material.
When bottom of bottle 103 is funnel-shaped structure, the small end of bottom of bottle 103 opens wide, and is provided at the small end and closes the bottle
The closure member at bottom 103, closure member form the second easy puncture portion 1032.
In addition, in order to enable entire specimen bottle 100 can be placed steadily, the small end of bottom of bottle 103 is downwardly extending and seals
First extension 1031 of closing member cooperation, the bottom of the first extension 1031 can smoothly support entire specimen bottle 100.
First extension 1031 can be several spaced apart structures, alternatively, the first extension 1031 opens for both ends
The hollow cylinder opened, and closure member is the matched cock body of inner wall with the first extension 1031.
Closure member is easily is pierced through by syringe needle, and flexible rubber system obtains.Closure member uses flexible rubber
It is made, being still closed part when lancet puncture enters in bottle 101, around puncture needle closely squeezes, and in puncture needle
When extracting closure member out, the hole being pierced on closure member can be extruded closing again, that is to say, that either still exist in piercing process
After the completion of puncture, closure member all has reliable sealing effect, further ensure that 101 interior or exterior space of bottle will not be mutual
Pollution.
The thickness of closure member is 6mm~10mm.Inventor has found, uses closure member of the thickness for 6mm~10mm, is realizing
While good sealing effect, also puncture needle is smoothly pierced through.
In order to further improve the stability for placing specimen bottle 100,101 side wall of bottle extends to form downwards the second extension
Portion 1011.Due to the second extension 1011 the axial line distance apart from bottom of bottle 103 farther out, therefore, it is possible to increase specimen bottle 100
Actual support area, so as to improve the smoothness in 100 placement process of specimen bottle.
In order to improve the intensity of specimen bottle 100, the outer wall of the inner wall of the second extension 1011 and the first extension 1031 it
Between be connected with several reinforcing plates 108, reinforcing plate 108 is divided into several spaces being connected to each other with follow-up rotary components 506.
In order to further improve the intensity of specimen bottle 100, reinforcing plate 108 extends downwardly, and with the first extension 1031
Outer wall connects.Strengthen the bonding strength between bottom of bottle 103, the first extension 1031 and the second extension 1011, further carry
The high structural strength of the present embodiment bottle 101, ensures that bottle 101 has good reliability；On the other hand, if by setting
Dry reinforcing plate 108, each 108 and first extension 1031 of reinforcing plate and the second extension 1011 are formed and centrifugation apparatus or shake
Swing the matched structure of hold assembly of equipment so that, after bottle 101 is placed into centrifugation apparatus or concussion equipment, energy
It is enough reliably to be clamped and fixed, in this way, also ensure Precerving liquid in bottle 101 and cell sample has good mixing
Effect.
In the embodiment of the present invention, filter device 104 is strainer, and the filtering accuracy of strainer is 8 μm~11 μm.By filter device
104 are arranged to the strainer that filtering accuracy is 8 μm~11 μm, that is, ensure that cell-preservation liquid can smoothly thoroughly after dissolved impurity
It crosses, in turn avoids passing through for effective cell.
Fig. 3 and Fig. 7 are referred to, concentration mixing mechanism further includes load maintainer 200, load maintainer in the embodiment of the present invention
200 include：Sample disk 201 is provided with to place the loading hole 2011 of specimen bottle 100 on sample disk 201.It is loaded by setting
Hole 2011 can axially support specimen bottle 100, and limitation specimen bottle 100 rocks.
Fig. 4 and Fig. 7 are referred to, in embodiments of the present invention, which further includes limitation specimen bottle 100 and beat
Position-limit mechanism 300.When specimen bottle 100 is sent to concentration position by load maintainer 200, limited under the action of position-limit mechanism 300
The bounce of bottle cap 102 processed.
The concentration position of the position-limit mechanism 300 is provided with rotatable limited block 303, and limited block 303 can be with 102 phase of bottle cap
It abuts.The position-limit mechanism 300 is set directly at the top of concentration mixing mechanism 500.It can realize that limited block 303 is arranged on concentration
The form of 500 top of mixing mechanism has very much, and the embodiment of the present invention specifically introduces one kind, which includes：It sets vertically
The support plate 301 put；And the limiting plate 302 in support plate 301 is vertically set on, limited block 303 is arranged on limiting plate 302
On.
In order to reduce frictional force of the limited block 303 in rotation process, smoothness in 303 operational process of limited block is improved,
Limited block 303 is arranged on by bearing 304 on limiting plate 302, and the outer ring of bearing 304 is fixed on limiting plate 302, limited block
303 are fixed on the inner ring of bearing 304.
In order to optimize said program, limited block 303 be provided with 102 matched convex block of the bottle cap of specimen bottle 100, when dense
When contracting mixing mechanism 500 drives 100 high-speed cruising of specimen bottle (including high speed rotation or high frequency oscillation), in the restriction effect of convex block
Under, specimen bottle 100 is not susceptible to beat.
Well 305 is provided in the middle part of convex block, bottle cap is punctured to facilitate, is loaded into the first cavity 105.Due to sample
It needs to be loaded or sample in bottle 100, therefore, well 305 is set on convex block, it need not be by sample when being loaded or being sampled
This bottle 100 is transferred to other positions, reduces transmission technique, realizes that automatic sample and sampling provide support to be follow-up.Due to
The embodiment of the present invention by position-limit mechanism 300 carry out /V, specimen bottle 100 when carrying out mixing operation, 100 both ends of specimen bottle by
Power, stress is than more uniform.
The effect of first lifting assembly 501 is that concentration pin 5014 is transported to designated position, as long as the effect can be realized
Structure in the protection domain of the embodiment of the present invention.The embodiment of the present invention specifically discloses a kind of first lifting assembly 501
Concrete structure, first lifting assembly 501 include first straight line guide rail 5011；It is slidably matched with first straight line guide rail 5011
First sliding block 5012, lift side of first sliding block 5012 as the first lifting assembly 501；And the first sliding block 5012 of driving is run
First straight line motor 5013.
When will carry out cell concentration, controller control first straight line motor 5013 is run, and the first sliding block 5012 is first
Under the cooperation of linear motor 5013 and first straight line guide rail 5011, move, be arranged on to close to the direction of 100 bottom of specimen bottle
When concentration pin 5014 on first sliding block 5012 runs to appropriate location, the bottom of specimen bottle 100 is punctured, realizes specimen bottle
100 conducting with concentrating pin 5014.
Pumping operation and reversed air blowing operation of the effect switching of commutation component 504 to specimen bottle 100, as long as can realize
The structure of switched conductive is within the scope of the present invention.When the component 504 that commutates is reversal valve, concentration pin 5014 passes through pipe
Road is connected with the outlet A1 of reversal valve, and pumping components 502 are connected with the first import P1 of reversal valve, air blowing component 503 and commutation
The second import P2 connections of valve, when reversal valve is located at first state, the first import P1 of reversal valve and the outlet A1 of reversal valve
Conducting, the second import P2 of reversal valve and the outlet A1 of reversal valve are non-conduction；When reversal valve is located at the second state, reversal valve
The outlet A1 of first import P1 and reversal valve is non-conduction, and the second import P2 of reversal valve is turned on the outlet A1 of reversal valve.
The effect of pumping components 502 is that the invalid liquid in the second cavity 106 is released, to reach the mesh of cell concentration
, as long as can realize that the structure of suction action is within the scope of the present invention.
The effect of air blowing component 503 is reversely blown to the second cavity 106, due to sample in specimen bottle 100 mistake
It,, can be with by the way that air blowing component 503 is set reversely to blow filter device 104 there are the possibility of blocking filtering device 104 during filter
Filter device 104 is dredged, cell enrichment process can be accelerated.As long as the structure of blowing action can be realized in the guarantor of the present invention
In the range of shield.Preferably, pumping components 502 be peristaltic pump, the vacuum pump of air blowing component 503.
When carrying out cell concentration, controller controls the first lifting assembly 501 to run, and the first lifting assembly 501 drives sample
This bottle 100 is close, until puncturing bottom of bottle 103.Controller control commutation Vavle switching is in first state, pumping components 502 with it is dense
Shortening 5014 turns on, and pumping components 502 are run, and the invalid liquid in specimen bottle 100 is pumped out, when running to preset time
When, controller control commutation Vavle switching is simultaneously in the second state, at this point, air blowing component 503 is turned on concentration pin 5014, air blowing group
Part 503 is run, and is reversely blown into specimen bottle 100, and the effective cell for blocking filter device 104 in specimen bottle 100 is blown away.It changes
Switch repeatedly to valve, so as to which suction and air blowing operation be repeated.Due to being provided with air blowing component 503 in the embodiment of the present invention,
Therefore, in cell concentration, the filter device 104 for blocking specimen bottle 100 and concentration pin 5014 can be effectively prevented, is being added
While fast cell enrichment, cell superposition can be efficiently reduced.
, it is necessary to carry out mixing operation to specimen bottle 100 after Cell Buffer is added in into the first cavity, in order to simplify knot
Structure, the concentration mixing mechanism 500 in the embodiment of the present invention can also perform mixing operation, at this point, the concentration mixing mechanism also wraps
It includes：
Rotary components 506 are able to carry out mixing operation；And
Second lifting assembly 505 can drive rotary components 506 to close to the movement of the direction of specimen bottle 100 and and bottom of bottle
103 docking；
After Cell Buffer is added in the first cavity 105, controller controls the second lifting assembly 505 to run, and controls
Rotary components 506 perform mixing operation after being docked with bottom of bottle 103.
After Cell Buffer is added in into the first cavity 105, the second lifting assembly 505 drives in the controller of controller
Rotary components 506 are moved to close to the direction of specimen bottle 100, and rotary components 506 are docked with bottom of bottle 103, controller control rotation
Component 506 is run, and drives 100 high-speed cruising of specimen bottle, which includes rotation at a high speed or shake at a high speed, to reach mixed
The purpose of even effective cell and Cell Buffer.
The effect of second lifting assembly 505 is that rotary components 506 are transported to designated position, as long as the effect can be realized
Structure all falls in the scope of protection of the present invention.A kind of specific knot of second lifting assembly 505 is specifically disclosed in the embodiment of the present invention
Structure.Second lifting assembly 505 includes：Second straight line guide rail 5051；It is slided with second straight line guide rail 5051 is slidably matched second
Block 5052, lift side of second sliding block 5052 as the second lifting assembly 505；And the second of driving the second sliding block 5052 operation
Linear motor 5053.
The effect of rotary components 506 is to provide high speed centrifugation power for specimen bottle 100, as long as high-speed rotating knot can be realized
Structure is in the protection domain of the embodiment of the present invention.A kind of the specific of rotary components 506 is specifically disclosed in the embodiment of the present invention
Structure, the rotary components 506 include：The electric rotating machine being fixed on the second sliding block 5052 is arranged on the driving end of electric rotating machine
Rotation shift fork 5061, the rotation effect of shift fork 5061 is realized docks with the bottom of bottle 103 of specimen bottle 100.
Alternatively, transmission component 5062 is additionally provided between electric rotating machine and rotation shift fork 5061, for example, the transmission component
5062 be gear-driven assembly 5062.In order to optimize said program, the rotation shift fork 5061 in the embodiment of the present invention is grabbed for three to be dialled
Fork, three grab shift fork uniform force application, and further three grab shift fork three, which grab, to be uniformly arranged.
When needing to carry out mixing to specimen bottle 100, specimen bottle 100 is transmitted to dense by controller control load maintainer 200
It condenses, and second straight line motor 5053 is controlled to bring into operation, the driving end of second straight line motor 5053 drives the second sliding block 5052
Along being moved to close to the direction of limited block 303 until holding out against for second straight line guide rail 5051.Electric rotating machine brings into operation, sample
Rotation shift fork 5061 of the bottle 100 on electric rotating machine rotates at a high speed under driving, until effective cell mixes with Cell Buffer
It is even.
When further, due to the use of the concentration mixing mechanism 500 in the embodiment of the present invention, mixing after first concentrating, therefore,
In order to avoid performing influencing each other between the equipment of above-mentioned operation.The middle part of rotation shift fork 5061 is provided with receiving concentration pin
The 5014 concentration holes passed through.
When concentrating operation, after the second lifting assembly 505 runs to designated position, rotation shift fork 5061 and specimen bottle 100
Bottom of bottle 103 coordinates, and after the first lifting assembly 501 runs to designated position, concentration pin 5014 is through concentration hole and punctures specimen bottle
100 bottom of bottle 103 performs pumping operation and reversed air blowing operation repeatedly；First lifting assembly 501 is retracted, and concentration pin 5014 pulls out
Go out；Cell Buffer is added in into specimen bottle 100, rotary components 506 bring into operation, under the action of shift fork 5061 is rotated, sample
This bottle 100 rotates at a high speed, so as to which Cell Buffer be uniformly mixed with effective cell.Due to use more than arrangement form, cell
Enrichment is independent of each other with mixing, so as to save the part-time in equipment handoff procedure.
Referring to Fig. 8, in order to reduce human interference, sample charging mechanism 600, sample charging mechanism are further included in the embodiment of the present invention
600 can puncture bottle cap 102, and add in Cell Buffer into the first cavity 105.
Sample charging mechanism 600 includes plunger pump 601, solenoid valve 602 and sample needle 603, and sample needle 603 passes through pipeline and electromagnetism
The first outlet A2 connections of valve 602, the second outlet B2 of solenoid valve 602 are connected with Cell Buffer, the import P3 of solenoid valve 602
It is connected with plunger pump 601；When solenoid valve 602 is located at the third state, first outlet A2 and the solenoid valve 602 of solenoid valve 602
Import P3 is non-conduction, and the second outlet B2 of solenoid valve 602 is turned on the import P3 of solenoid valve 602；When solenoid valve 602 is located at the 4th
During state, the first outlet A2 of solenoid valve 602 is turned on the import P3 of solenoid valve 602, second outlet B2 and the electricity of solenoid valve 602
The import P3 of magnet valve 602 is non-conduction.
When needing to inject Cell Buffer into specimen bottle 100, sample needle 603 punctures the bottle cap 102 of specimen bottle 100,
Solenoid valve 602 switches and in the third state, and Cell Buffer is turned on plunger pump 601, the positive operation of plunger pump 601, will be thin
Born of the same parents' buffer solution is sucked into plunger pump 601；Solenoid valve 602 switches and in the 4th state, and sample needle 603 is led with plunger pump 601
Logical, Cell Buffer is pushed into specimen bottle 100 by 601 inverted running of plunger pump.
Due to needing to puncture the bottle cap 102 of specimen bottle 100 in 600 operational process of sample charging mechanism in the embodiment of the present invention,
The stab action artificially performs or automated execution.When automated execution, sample charging mechanism 600, which further includes, pushes away sample component, pushes away sample component
Driving end sample needle 603 is pushed into the bottle cap 102 of specimen bottle 100.The structure for pushing away sample component can refer to the first lifting group
The concrete structure of 501 and second lifting assembly 505 of part, is not repeated herein.
The foregoing description of the disclosed embodiments enables professional and technical personnel in the field to realize or use the present invention.
A variety of modifications of these embodiments will be apparent for those skilled in the art, it is as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, it is of the invention
The embodiments shown herein is not intended to be limited to, and is to fit to and the principles and novel features disclosed herein phase one
The most wide scope caused.
Claims (9)
1. a kind of concentration mixing mechanism, which is characterized in that including：
Specimen bottle, including bottle and with the matched bottle cap of the bottle, be provided with filter device, the bottle in the bottle
Body, the bottle cap and institute's filter device form the first cavity, and the bottom of bottle and institute's filter device of the bottle form the second cavity；
First lifting assembly can drive concentration pin to puncture bottom of bottle；
Pumping components are able to carry out pumping operation；
Air blowing component is able to carry out reversed air blowing operation；
Commutate component, can realize the pumping components and the conducting for concentrating pin and the air blowing component and the concentration pin
Conducting switching；And
Controller, the controller control the first lifting assembly to drive the direction of concentration pin to the close specimen bottle mobile and pierce
Broken bottom of bottle into second cavity, and control commutation switch between components pumping components and air blowing component perform repeatedly pumping operation and
Reversed air blowing operation.
2. concentration mixing mechanism as described in claim 1, which is characterized in that further include the position restrainer for limiting the bottle cap bounce
Structure.
3. concentration mixing mechanism as claimed in claim 2, which is characterized in that the concentration position of the position-limit mechanism is provided with and can revolve
The limited block turned, the limited block can be abutted against with the bottle cap.
4. concentration mixing mechanism as described in claim 1, which is characterized in that further include load maintainer, the load maintainer bag
Sample disk is included, is provided with to place the loading hole of specimen bottle on the sample disk.
5. concentration mixing mechanism as described in claim 1, which is characterized in that described dense when the commutation component is reversal valve
Shortening passes through pipeline and the outlet of the reversal valve, the first inlet communication of the pumping components and the reversal valve, institute
The second inlet communication of air blowing component and the reversal valve is stated, when the reversal valve is located at first state, the reversal valve
First import is turned on the outlet of the reversal valve, and the second import and the outlet of the reversal valve of the reversal valve are non-conduction；
When the reversal valve is located at the second state, the first import and the outlet of the reversal valve of the reversal valve are non-conduction, described
Second import of reversal valve is turned on the outlet of the reversal valve.
6. concentration mixing mechanism as described in claim 1, which is characterized in that further include sample charging mechanism, the sample charging mechanism energy
It enough punctures bottle cap and adds in Cell Buffer into first cavity；
The concentration mixing mechanism can also perform mixing operation, described after Cell Buffer is added in first cavity
Controller control concentration mixing mechanism performs mixing operation.
7. concentration mixing mechanism as claimed in claim 6, which is characterized in that the sample charging mechanism includes plunger pump, solenoid valve
And sample needle, the sample needle are connected by pipeline with the first outlet of the solenoid valve, the second outlet of the solenoid valve with
Cell Buffer connects, and the import of the solenoid valve is connected with the plunger pump；
When the solenoid valve is located at the third state, the first outlet of the solenoid valve and the import of the solenoid valve are non-conduction,
The second outlet of the solenoid valve is turned on the import of the solenoid valve；
When the solenoid valve is located at four states, the first outlet of the solenoid valve is turned on the import of the solenoid valve, institute
Second outlet and the import of the solenoid valve for stating solenoid valve are non-conduction.
8. concentration mixing mechanism as claimed in claim 7, which is characterized in that the sample charging mechanism, which further includes, pushes away sample component, institute
It states sample needle described in pushing away sample Component driver and punctures bottle cap.
9. concentration mixing mechanism as claimed in claim 6, which is characterized in that the concentration mixing mechanism further includes：
Rotary components are able to carry out mixing operation；And
Second lifting assembly can drive the direction of rotary components to the close specimen bottle mobile and be docked with the bottom of bottle；
After Cell Buffer is added in the first cavity, the controller controls the second lifting assembly operation, and controls institute
State execution mixing operation after rotary components are docked with the bottom of bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810094206.3A CN108106922A (en) | 2018-01-31 | 2018-01-31 | A kind of concentration mixing mechanism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810094206.3A CN108106922A (en) | 2018-01-31 | 2018-01-31 | A kind of concentration mixing mechanism |
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| CN108106922A true CN108106922A (en) | 2018-06-01 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810094206.3A Pending CN108106922A (en) | 2018-01-31 | 2018-01-31 | A kind of concentration mixing mechanism |
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| CN (1) | CN108106922A (en) |
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| CN112697570A (en) * | 2020-11-27 | 2021-04-23 | 迈克医疗电子有限公司 | Test tube taking, placing and mixing device and sample analyzer |
| CN113009150A (en) * | 2021-02-20 | 2021-06-22 | 北京华科泰生物技术股份有限公司 | Concentration device for collecting urine microalbumin in sweat, detection kit comprising same and application thereof |
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