CN107991221A - Optical fiber type microparticle detects and method of counting and system - Google Patents

Optical fiber type microparticle detects and method of counting and system Download PDF

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Publication number
CN107991221A
CN107991221A CN201711249865.1A CN201711249865A CN107991221A CN 107991221 A CN107991221 A CN 107991221A CN 201711249865 A CN201711249865 A CN 201711249865A CN 107991221 A CN107991221 A CN 107991221A
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China
Prior art keywords
optical fiber
microparticle
counting
signal
array
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CN201711249865.1A
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Chinese (zh)
Inventor
黄战华
郎明远
张�林
蔡怀宇
张尹馨
李凤娇
潘成
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Tianjin University
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Tianjin University
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Publication of CN107991221A publication Critical patent/CN107991221A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1484Electro-optical investigation, e.g. flow cytometers microstructural devices
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G01N2015/1452Adjustment of focus; Alignment
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1481Optical analysis of particle in droplet
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1486Counting the particles

Abstract

The present invention relates to a kind of microparticle optical detecting method, to propose the method and system being detected using fiber optic pin technique to microparticle with counting with high-resolution and stability.The present invention, optical fiber type microparticle detects and method of counting and system, by substrate fixed laser, the position of microparticle plate and array fibre, on the micro channel array in collimated laser beam vertical incidence microparticle version that light source is sent, fibre core in optical fiber probe array per a branch of optical fiber is directed at corresponding microparticle passage, when there is no microparticle, collimated laser beam is directed through the fibre core that microchannel enters optical fiber, when there is microparticle to enter above fibre core, for transparent grain, the light only a small part of oil droplet can enter fibre core and form the transmission of stable mode through in transparent grain;For nontransparent particle, particle blocks light completely;Photodetector, which counts, is sent into the final counting of counting circuit completion.Present invention is mainly applied to microparticle optical detection occasion.

Description

Optical fiber type microparticle detects and method of counting and system
Technical field
The present invention relates to a kind of microparticle optical detecting method, more particularly to a kind of micro- based on fiber optic pin technique Grain detection and method of counting and system.
Background technology
Microparticle is counted has extremely important work to fields such as medical diagnosis, biological study, the monitoring of hygiene, industrial productions With.The main method counted at present to microparticle in liquid has two kinds, and one kind is laser light blockage method, and photodetector is direct The light by testing liquid that is sent by laser light source is received, judges oil droplet by the way that contrast whether there is the power of oil droplet optical signal By the way that to complete to count, but since the environmental factors such as bias light are disturbed, the loss during light propagation, receives detector To light energy substantially weaken so that counting precision is low and the requirement of higher is proposed to light source and detector performance;It is another Kind is the method for counting based on micro-image, reaches computer by image in several microcode microscope acquisition channels, utilizes image Treatment technology counts oil droplet in passage, and there are the shortcomings of speed is slow, precision is low, universality is low and real-time is poor.
Optical fiber is a kind of optical fiber of special construction, is made of the fibre core at center and the covering of periphery, light is wherein Transmission is the total reflection based on light, so light energy is limited and concentrated in fibre core in transmitting procedure, and the biography that covering is light Defeated offer reflecting surface and optically isolated, therefore optical fiber is optimal optical transmission medium.Fibre optical sensor is to send out rapidly in recent decades A kind of novel sensor that exhibition is got up, basic fibre optical sensor are made of light source, optical fiber and detector, are broadly divided into two classes: One kind is known as light transmission type sensor, its optical fiber only plays the role of transmission;It is another kind of to be known as sensing type sensor, its optical fiber Have sensitive and transmission effect concurrently to measured signal.Compared with other sensors, fibre optical sensor has high sensitivity, low-loss With the series of advantages such as fast response time, therefore it is widely used in multiple necks such as human medical, urban construction monitoring, industry monitoring Domain, especially plays irreplaceable effect in terms of the accurate measurement such as environmental monitoring and clinical medicine detection.
The content of the invention
For overcome the deficiencies in the prior art, the present invention is directed to propose being visited using optical fiber with high-resolution and stability Pin technology is detected microparticle the method and system with counting.The technical solution adopted by the present invention is optical fiber type microparticle Detection and method of counting, by the position of substrate fixed laser, microparticle plate and array fibre, collimated laser beam that light source is sent On micro channel array in vertical incidence microparticle version, the fibre core alignment in optical fiber probe array per a branch of optical fiber is micro- corresponding Grain passage, when not having microparticle, collimated laser beam is directed through the fibre core that microchannel enters optical fiber, fine when there is microparticle to enter When above core, for transparent grain, the light only a small part of oil droplet can enter fibre core and shape through in transparent grain Into the transmission of stable mode, the seldom part refers to meet optical fiber incident angle and reaches core region;For nontransparent Particle, particle block light completely, and no light can enter fibre core;Photodetector is installed in the exit end of optical fiber, Photodetector output signal progress signal processing is obtained into pulse square wave signal when oil droplet passes through, counting circuit is sent into and completes It is final to count.
According to microparticle kinetic characteristic and environmental factor, select to carry out illumination laser the frequency of high frequency modulated.
The processing that the signal exported to the photodetector carries out includes high-frequency ac amplification, filtering and voltage Compare.
The processing that number carries out counting to pulse square wave letter includes CPLD logic circuits and is counted and latched, by ARM Or cpu data interface reads count value.
When environment complex jamming is strong, increase refers to detection channels, the optical fiber align microchannel side of the passage all the way Non- oil droplet region, the detectable signal of all oil droplets with this with reference to detectable signal carry out differential amplification, with eliminate disturb and make an uproar Sound.
Optical fiber type microparticle detects and number system, including lighting source, microparticle plate, substrate, array fibre, photoelectricity are visited Survey device, be connected with the photodetector output terminal and be used for be by the photoelectric sensor institute output light electric current signal processing The signal processing circuit being made of amplifying circuit, filter circuit and voltage comparator circuit of pulse square wave signal and the signal Process circuit is connected and is used to carry out being made of programming device and processor for step-by-step counting to the pulse square wave signal Counting circuit.
Illumination laser and the array fibre probe uses plug-in structure.
The path of microchannel on the microparticle plate carries out targeted design, is arranged in detection zone equidistant parallel;If There is the position alignment of laser socket and array fibre socket mark.
The substrate reserves the mounting groove of illumination laser and array fibre probe when manufacturing and designing.
The signal processing circuit includes high-frequency ac automatic gain amplification agc circuit, the High frequency filter electricity being sequentially connected Road, voltage comparator circuit, by photodetector output signal by high-frequency ac amplify and filter obtain it is low when oil droplet passes through Frequency signal, pulse square wave signal is obtained by voltage comparator.
Programming device is CPLD or FPGA, and processor is embedded processors ARM or computer processor CPU, CPLD Logic circuit by data-interface to per being counted and being latched all the way, the count value on every road is read by ARM or CPU.
The features of the present invention and beneficial effect are:
(1) present invention utilizes optical fiber sensing technology, a pair of with microchannel one per beam optical fiber because optical fiber has small core diameter Should, only by corresponding microchannel and meet that the light of specific incident angle could enter, improve sensitivity and counting Precision.
(2) light source and array fibre probe are designed to the module of functional independence by the present invention, can be configured as needed to The unit of various number of active lanes, is easy to produce in batches, debug and safeguard, and small, light weight, is easily installed and integrates.
(3) microchannel is corresponded per beam optical fiber in array fibre in the present invention, counting can be carried out at the same time to multiple passages.
(4) present invention will mechanically and electrically sub-circuit cure, and whole system is steady without mechanical movement and adjustment, work using process It is fixed reliable.
(5) whole process Parameters Optimal Design of the present invention, from the mounting groove and alignment mark of substrate, to array fibre probe Encapsulation, to the signal processing system of series, all front and rear comprehensive design, realizes parameter matching optimization.
(6) present invention generation is low with installation and debugging cost, and laser, optical fiber and photodetector have benefited from current maturation Fiber communication market, function admirable and cost is low.
Brief description of the drawings:
Fig. 1 is system structure and workflow schematic diagram.
In figure, 101:Lighting source;102 microparticle plates;103:Substrate;104:Array fibre;105:Photodetector; 106:Filter circuit;107:Amplifying circuit;108:Voltage comparator circuit;109:CPLD or FPGA device;110:ARM or CPU devices Part.
Fig. 2 is optical fiber type microparticle detection technique schematic diagram.
In figure, 201:Microparticle;202:Microparticle plate;203:Fiber core;204:Fibre cladding;205:Photodetection Device;206:Substrate;207:Illuminate collimated laser beam.
The nontransparent particles of 2A are by the way that 2B is without particle by the way that transparent grain (oil droplet class) is by the way that transparent grain (bubble class) is logical Cross.
Fig. 3 is direct detection signal waveform.
Fig. 4 is principles of signal processing schematic diagram.In figure:
Detectable signal waveform after the adjustment of 4A high frequencies, the signal waveform after 4B High frequency amplifications, the signal after 4C High frequency filters Waveform, Fig. 4 D count impulse waveform for oil droplet.
Fig. 5 is structure scheme of installation.
In figure, 501:Microchannel and microparticle array;502:Fiber array;503:Light source socket;504:Substrate;505:Light Fine socket;506:Assembly alignment marks;507:Light source positions;508:Fiber orientation groove;509:Collimated laser beam.
Embodiment
The shortcomings that in order to overcome existing microparticle counting technology and deficiency, the present invention provides one kind to have high-resolution With the method and system being detected using fiber optic pin technique to microparticle with counting of stability.
Technical solution of the present invention is as follows:
Optical fiber probe declines particle detections and method of counting, according to microparticle kinetic characteristic and environmental factor, chooses whether High frequency modulated is carried out to illumination laser, by the relative position of substrate fixed light source, microparticle plate and array fibre, laser hair On the micro channel array in collimated laser beam vertical incidence microparticle version gone out, the fibre core in optical fiber probe array per a branch of optical fiber It is directed at microparticle passage.When not having microparticle, collimated laser beam is directed through the fibre core that microchannel enters optical fiber, when there is micro- When grain is entered above fibre core, for transparent grain (drop, oil droplet, bubble etc.), through the light only a small part of oil droplet (meet optical fiber incident angle, and reach core region) can enter fibre core and form the transmission of stable mode, for nontransparent Particle, particle block light completely, and no light can enter fibre core.Photodetector is installed in the exit end of optical fiber, Photodetector output signal progress signal processing is obtained into pulse square wave signal when oil droplet passes through, counting circuit is sent into and completes It is final to count.
The laser illuminator source decides whether to carry out high frequency modulated according to microparticle kinetic characteristic and environmental factor.
The probe portion of the lighting source, array fibre and photodetector composition, optical fiber have small core diameter, per beam Optical fiber and microchannel correspond, only by corresponding microchannel and meet that the light of specific incident angle could enter.
The lighting source, array fibre and photodetector composition probe portion, can to multichannel microchannel at the same time into Row counts.
The processing that the signal exported to the photodetector carries out includes high-frequency ac amplification, filtering and voltage Compare.
The processing that number carries out counting to pulse square wave letter includes CPLD logic circuits and is counted and latched, by ARM Or cpu data interface reads count value.
Optical fiber probe declines particle detections and number system, it include lighting source, microparticle plate, substrate, array fibre, Photodetector, be connected with the photodetector output terminal and be used for photoelectric sensor institute output light current signal Handle the signal processing circuit being made of amplifying circuit, filter circuit and voltage comparator circuit for pulse square wave signal and institute State signal processing circuit be connected and be used to carrying out the pulse square wave signal step-by-step counting by FPGA or CPLD devices and The counting circuit that ARM or CPU devices are formed.
The illumination laser 101 and array fibre probe 104 use plug-in structure.
The path of microchannel on the microparticle plate 102 carries out targeted design, is arranged in detection zone equidistant parallel; Design processes the position alignment mark of laser socket and array fibre socket.
The substrate 103 reserves the mounting groove of illumination laser and array fibre probe when manufacturing and designing.
The light source and array fibre will be designed to the module of functional independence, can be configured to the list of various number of active lanes Member, realizes Multi-channel matrix.
The light source and array fibre are small, light-weight, and array fibre modularization integrates light path.
The signal processing circuit includes high-frequency ac AGC (automatic gain amplification) circuit, the High frequency filter being sequentially connected Circuit, voltage comparator circuit, photodetector output signal is amplified by high-frequency ac and filtering is obtained when oil droplet passes through Low frequency signal, pulse square wave signal is obtained by voltage comparator.
The pulse-scaling circuit is sequentially connected CPLD or FPGA devices and ARM or CPU devices, and CPLD logic circuits are to each Road is counted and is latched, and reads the count value on every road by data-interface by ARM or CPU.
The present invention is described in detail with reference to the accompanying drawings and detailed description, is not the experiment model the present invention Enclose and be confined to this.
As shown in Figure 1, high frequency modulated 111 is carried out to illumination laser 101 by CPLD or FPGA device 108, through ovennodulation The near-infrared collimated laser beam for the 1310 or 1550nm wavelength that laser 101 afterwards is sent impinges perpendicularly on microparticle plate 102 On microchannel, and the typical core diameter of the Single mode communication optical fiber of 1310 or 1550nm wavelength is 8~11 μm, and end face is direct in atmosphere The numerical aperture NA representative values of coupling are 0.13, ± 4 ° of angular aperture are approximately corresponding to, as shown in Fig. 2, the light into covering 204 Line cannot stablize transmission in a fiber, be depleted rapidly, and the light of fibre core 203 is only entered less than aperture angle and could be passed It is defeated, even if the light beam more than the angle can not form transmission mode into fibre core, it is depleted rapidly.Fixed by substrate 206, Array fibre probe is set closely to connect the microparticle passage of accurate 10 μm wide of the alignment of fibre core of microparticle plate and probe.Such as Fig. 2 B Shown, when no microparticle 201, collimated laser beam 207 is directed through microchannel 202, into the fibre core 203 of optical fiber.Into Fibre core and the ratio and the shape of passage, the refractive index of solution and substrate, absorptivity for forming the optical fiber of stable mode transmission, and The factors such as alignment precision are related.When there is microparticle 201 to enter 203 top of fibre core, for the transparent microparticle of oil droplet class:Such as Fig. 2 C It is shown, since oil droplet class microparticle is different from the refractive index of solution, and there is spherical, therefore for example same short focus of oil droplet is saturating Mirror, dissipates again after directional light is converged, and the angle of divergence is very big, in this way, light only a small part (the convergence angle through oil droplet Less than ± 4 °, and reach core region) fibre core 203 can be entered and form the transmission of stable mode;It is transparent micro- for bubble class Particle:As shown in Figure 2 D, bubble inner refractive index is less than outside liquid, therefore light propagation condition and oil droplet are on the contrary, directional light First dissipate and converge again;For nontransparent microparticle:As shown in Figure 2 C, particle 201 blocks light completely, and no light can be into Enter fibre core 203.Photodetector 205 is installed in the exit end of optical fiber, the photosignal of detector is as shown in figure 3, there is oil droplet By when, the signal of detector diminishes, no oil droplet by when, the photosignal of detector is held essentially constant, and so passes through spy Passing through for oil droplet can be detected by surveying the photosignal of device.
Photodetector output signal entering signal process circuit, by AGC (automatic gain amplification) 106 and High frequency filter Pulse square wave signal when oil droplet passes through is obtained after 107 compared with voltage comparator 108, oil droplet counting pulse signal enters FPGA or CPLD devices 109 are counted and latched, and read the counting on every road by data-interface by ARM or CPU devices 110 Value.
When environment complex jamming is strong, it can increase and refer to detection channels all the way, the optical fiber align microchannel of the passage The non-oil droplet region on side.The detectable signal of all oil droplets carries out differential amplification with this with reference to detectable signal, it is possible to eliminates The overwhelming majority interference of environment and noise, the more stable and accurate process signal for detecting oil droplet.
The present invention is larger in view of the velocity variations that pass through due to oil droplet when photodetector output signal is handled And frequency is low, at the same time containing the various environmental factors such as temperature, vibration, electromagnetic interference, make the absolute value generation of photosignal larger Low-frequency fluctuation, and with the band overlapping of oil droplet signal, therefore cannot simply directly use fiber-optical probe original output Signal carries out threshold value judgement, therefore the system carries out high frequency modulated to illumination laser.After high frequency modulated, according to modulating frequency, Detectable signal can accurately be amplified.Concrete signal processing scheme is as follows:The system carries out high frequency modulated to illumination laser 101, The process speed of oil droplet is generally less than 10KHz, and is selected in MHz in view of the needs of AGC (automatic gain amplification), modulating frequency In rank.Since the frequency of fiber optic communication is all more than 100MHz, parts selection readily satisfies requirement.Fig. 4 A are using tune The photosignal waveform obtained after laser illumination processed, the signal pass through the amplified waveform of high-frequency ac as shown in Figure 4 B, by In employing high frequency modulated, therefore AGC easy to implement, automatically according to the amplitude modulation gain of input signal, output control is set to exist In a certain range.The amplified signals of AGC are filtered by high frequency band group, filter high-frequency modulation signal, filtered is oil droplet warp Out-of-date low frequency signal, as shown in Figure 4 C, filtered signal pass through comparator, obtain the pulse square wave signal of oil droplet process such as Shown in Fig. 4 D, oil droplet counting pulse signal enters FPGA or CPLD devices 109.CPLD is counted and latched all the way to every, by ARM or CPU devices 110 read the count value per road by data-interface.
The present invention needs to ensure several essential conditions to realize above-mentioned effectively detection in structure:(1) fiber core essence Really alignment microchannel, the accuracy of alignment will influence the signal-to-noise ratio for light energy, that is, follow-up signal that fibre core receives.(2) fiber core As far as possible close to microchannel, fiber core is closer to microchannel, and the influence of diffraction effect is with regard to smaller, and non-signal light is also fewer, noise Than also higher.(3) incident light is directional light as far as possible, and the efficiency that directional light enters fibre core is highest, and being capable of more preferable area Divide in the converged light of oil droplet lens effect.(4) light source, optical fiber probe and microchannel and substrate consolidate connection, avoid vibration from causing Dislocation and distance change, disturb oil droplet photosignal stability.Based on these conditions, the preliminary structure design side of the system Case is as shown in Figure 5:(1) path of the microchannel on microparticle plate 501 carries out targeted design, is arranged in detection zone equidistant parallel Row.(2) mounting groove 507 of illumination laser and the mounting groove 508 of array fibre probe are reserved when substrate 504 manufactures and designs, side Just with the installation of later illumination laser and array fibre probe.(3) while processing in microchannel 501, design processes position Alignment mark 506 is put, can be by laser socket and the center of array fibre probe fine registration microchannel according to these marks. (4) illumination laser and plug-in type structure is used with array fibre probe 502, leaves light source socket 503 in structure and optical fiber is inserted Seat 505, relatively independent module is not only convenient to the large batch of processing and manufacturing of each component and detection, and be conducive to safeguard and more Change, while reduce manufacturing cost and use cost.
What above-mentioned optical fiber type microparticle detection technique method and system designed is the laser of 1310 or 1550nm, actually complete The laser of 808nm, 850nm can be used entirely, therefore the range of choice of laser and power is very wide, optical fiber and detector are substantially Can be general;The adaptable microparticle frequency of institute is actual up to hundreds of kHz from direct current to tens kHz;Due to using CPLD, Number of counter bits can easily reach 16 or 20 or 24 or 32, can be designed as required, fully can adapt to and expire The readout time cycle request of sufficient data;It can also pass through ARM's or CPU at the same time from data/address bus speedy carding process USB2.0 mouthfuls, RS232C, the wave point output such as Ethernet or various WIFI.
The technical concepts and features of above-mentioned embodiment only to illustrate the invention, can not limit the present invention's with this Protection domain.The equivalent change or modification that all Spirit Essences according to the present invention are done, should all cover in protection scope of the present invention Within.

Claims (10)

1. a kind of optical fiber type microparticle detection and method of counting, it is characterized in that, by substrate fixed laser, microparticle plate and array The position of optical fiber, on the micro channel array in collimated laser beam vertical incidence microparticle version that light source is sent, optical fiber probe array In fibre core per a branch of optical fiber be directed at corresponding microparticle passage, when not having microparticle, collimated laser beam is directed through micro- logical Road enters the fibre core of optical fiber, when there is microparticle to enter above fibre core, for transparent grain, and the light of oil droplet through in transparent grain Line only a small part can enter fibre core and form the transmission of stable mode, and the seldom part refers to meet that optical fiber enters Firing angle degree and reach core region;For nontransparent particle, particle blocks light completely, and no light can enter fibre core; Photodetector is installed in the exit end of optical fiber, when photodetector output signal progress signal processing is obtained oil droplet and passed through Pulse square wave signal, be sent into counting circuit and complete final count.
2. optical fiber type microparticle detection as claimed in claim 1 and method of counting, it is characterized in that, according to microparticle kinetic characteristic With environmental factor, select to carry out illumination laser the frequency of high frequency modulated.
3. optical fiber type microparticle detection as claimed in claim 1 and method of counting, it is characterized in that, to the photodetector The processing that the signal exported carries out includes high-frequency ac amplification, filtering and voltage and compares.
4. optical fiber type microparticle as claimed in claim 1 detection and method of counting, it is characterized in that, it is described to pulse square wave letter Number carrying out counting processing includes CPLD logic circuits and is counted and latched, by ARM or cpu data interface reading count value.
5. optical fiber type microparticle detection as claimed in claim 1 and method of counting, it is characterized in that, when environment complex jamming is strong When, increase and refer to detection channels all the way, the non-oil droplet region beside the optical fiber align microchannel of the passage, the spy of all oil droplets Survey signal and carry out differential amplification with reference to detectable signal with this, to eliminate interference and noise.
6. a kind of optical fiber type microparticle detection and number system, it is characterized in that, including lighting source, microparticle plate, substrate, array Optical fiber, photodetector, be connected with the photodetector output terminal and be used for photoelectric sensor institute output photoelectric Stream signal processing is electric for the signal processing being made of amplifying circuit, filter circuit and voltage comparator circuit of pulse square wave signal Road, be connected with the signal processing circuit and be used for pulse square wave signal progress step-by-step counting by programming device The counting circuit formed with processor.
7. optical fiber type microparticle detection as claimed in claim 5 and number system, it is characterized in that, the illumination laser and battle array Row optical fiber probe uses plug-in structure.
8. optical fiber type microparticle detection as claimed in claim 5 and number system, it is characterized in that, it is micro- on the microparticle plate The path of passage carries out targeted design, is arranged in detection zone equidistant parallel;Equipped with laser socket and array fibre socket Position alignment mark;The substrate reserves the mounting groove of illumination laser and array fibre probe when manufacturing and designing.
9. optical fiber type microparticle detection as claimed in claim 5 and number system, it is characterized in that, the signal processing circuit bag High-frequency ac automatic gain amplification agc circuit, high-frequency filter circuit, the voltage comparator circuit being sequentially connected are included, by photodetection Device output signal, which amplifies and filters by high-frequency ac, obtains low frequency signal when oil droplet passes through, and arteries and veins is obtained by voltage comparator Rush square-wave signal.
10. optical fiber type microparticle detection as claimed in claim 5 and number system, it is characterized in that, programming device CPLD Or FPGA, processor count all the way for embedded processors ARM or computer processor CPU, CPLD logic circuit to every And latch, count value of the data-interface reading per road is passed through by ARM or CPU.
CN201711249865.1A 2017-12-01 2017-12-01 Optical fiber type microparticle detects and method of counting and system Pending CN107991221A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114858707A (en) * 2022-03-15 2022-08-05 燕山大学 Plug-in combined optical fiber array sensor and gas-liquid two-phase flow parameter measuring method

Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040083798A1 (en) * 2002-11-05 2004-05-06 Sadar Michael J. Detection of filter breakthrough
CN1595112A (en) * 2004-06-25 2005-03-16 武汉理工大学 On-line oil monitoring sensor based on double light paths
JP2005221327A (en) * 2004-02-04 2005-08-18 Ngk Insulators Ltd Measuring instrument and its manufacturing device
CN1846126A (en) * 2003-08-05 2006-10-11 卢米尼克斯股份有限公司 Light emitting diode based measurement systems
CN101776599A (en) * 2003-08-14 2010-07-14 塞通诺米/St有限责任公司 Optical detector for a particle sorting system
CN102272574A (en) * 2010-03-31 2011-12-07 古河电气工业株式会社 Optical information analysis device and optical information analysis method
CN102334021A (en) * 2009-02-27 2012-01-25 贝克曼考尔特公司 Stabilized optical system for flow cytometry
CN102472702A (en) * 2009-07-07 2012-05-23 索尼公司 Microfluidic device
CN102868445A (en) * 2012-06-29 2013-01-09 中国人民解放军国防科学技术大学 Device and method for counting micro-particles based on micro-nanofiber
CN102998234A (en) * 2012-12-14 2013-03-27 江苏苏净集团有限公司 Micro liquid grain counter chip
CN202994624U (en) * 2012-12-06 2013-06-12 江苏苏净集团有限公司 Counting system of particle counter
CN104297155A (en) * 2014-09-28 2015-01-21 中国科学院长春光学精密机械与物理研究所 Multichannel parallel spectrum detection system
CN104330348A (en) * 2014-10-16 2015-02-04 中国科学院上海技术物理研究所 Blood corpuscle classification system based on flow-type super-continuum spectrum ringdown spectroscopy and method thereof
CN104677789A (en) * 2015-03-05 2015-06-03 江苏苏净集团有限公司 Nanoparticle counting detection device and method
CN105806816A (en) * 2016-03-15 2016-07-27 合肥工业大学 Detecting device and method for multichannel up-conversion luminescence

Patent Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040083798A1 (en) * 2002-11-05 2004-05-06 Sadar Michael J. Detection of filter breakthrough
CN1846126A (en) * 2003-08-05 2006-10-11 卢米尼克斯股份有限公司 Light emitting diode based measurement systems
CN101776599A (en) * 2003-08-14 2010-07-14 塞通诺米/St有限责任公司 Optical detector for a particle sorting system
JP2005221327A (en) * 2004-02-04 2005-08-18 Ngk Insulators Ltd Measuring instrument and its manufacturing device
CN1595112A (en) * 2004-06-25 2005-03-16 武汉理工大学 On-line oil monitoring sensor based on double light paths
CN102334021A (en) * 2009-02-27 2012-01-25 贝克曼考尔特公司 Stabilized optical system for flow cytometry
CN102472702A (en) * 2009-07-07 2012-05-23 索尼公司 Microfluidic device
CN102272574A (en) * 2010-03-31 2011-12-07 古河电气工业株式会社 Optical information analysis device and optical information analysis method
CN102868445A (en) * 2012-06-29 2013-01-09 中国人民解放军国防科学技术大学 Device and method for counting micro-particles based on micro-nanofiber
CN202994624U (en) * 2012-12-06 2013-06-12 江苏苏净集团有限公司 Counting system of particle counter
CN102998234A (en) * 2012-12-14 2013-03-27 江苏苏净集团有限公司 Micro liquid grain counter chip
CN104297155A (en) * 2014-09-28 2015-01-21 中国科学院长春光学精密机械与物理研究所 Multichannel parallel spectrum detection system
CN104330348A (en) * 2014-10-16 2015-02-04 中国科学院上海技术物理研究所 Blood corpuscle classification system based on flow-type super-continuum spectrum ringdown spectroscopy and method thereof
CN104677789A (en) * 2015-03-05 2015-06-03 江苏苏净集团有限公司 Nanoparticle counting detection device and method
CN105806816A (en) * 2016-03-15 2016-07-27 合肥工业大学 Detecting device and method for multichannel up-conversion luminescence

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114858707A (en) * 2022-03-15 2022-08-05 燕山大学 Plug-in combined optical fiber array sensor and gas-liquid two-phase flow parameter measuring method
CN114858707B (en) * 2022-03-15 2024-04-02 燕山大学 Plug-in type combined optical fiber array sensor and gas-liquid two-phase flow parameter measurement method

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