CN107950383A - A kind of small area producing method for seed of hybrid rice with the different female Combination nova of father - Google Patents

A kind of small area producing method for seed of hybrid rice with the different female Combination nova of father Download PDF

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Publication number
CN107950383A
CN107950383A CN201711298194.8A CN201711298194A CN107950383A CN 107950383 A CN107950383 A CN 107950383A CN 201711298194 A CN201711298194 A CN 201711298194A CN 107950383 A CN107950383 A CN 107950383A
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China
Prior art keywords
parent
male parent
female
spacing
phase
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CN201711298194.8A
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Chinese (zh)
Inventor
王爱民
孙明法
严国红
朱国永
唐红生
刘凯
施伟
张桂云
孙标
孙一标
赵绍路
宛柏杰
朱静雯
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Jiangsu Institute of Coastal Area Agricultural Sciences
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Jiangsu Institute of Coastal Area Agricultural Sciences
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Priority to CN201711298194.8A priority Critical patent/CN107950383A/en
Publication of CN107950383A publication Critical patent/CN107950383A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A kind of small area producing method for seed the present invention relates to hybrid rice with the different female Combination nova of father, comprises the following steps:Select the preferable field of isolation condition, the method wrapped using two is by the blossom-control technics of all variety classeses female parent within three male parent florescences phase, rationally determine spacing in the rows between parental, male parent line-spacing, male parent spacing in the rows, Parent line-spacing, maternal line-spacing, different female parents, suitably spray " 920 ", human assistance catches up with powder, male parent is removed in time, and clean pure keeping, in due course classification harvest.This method generally per acre can 1500 or so hybrid rice new combinations of the production of hybrid seeds, specific area is depending on the quantity of parental varieties, work efficiency improves tens times than current conventional method, synergy during abridged edition section, the seed quality of harvest is high, quantity foot, can fully meet that the advantage of New Hybrid Rice Combination and the appraisal of Comprehensive Traits are actually needed.

Description

A kind of small area producing method for seed of hybrid rice with the different female Combination nova of father
Technical field
Patent of the present invention is related to a kind of producing method for seed, particularly a kind of small area system of hybrid rice with the different female Combination nova of father Kind method.
Background technology
China's breeding of hybridized rice research is walked in the forefront in the world, has been bred as a large amount of new hybrid rice varieties, has been for China Grain-production to the world is made that huge contribution, greatly maintains the safety of grain-production.Ground in breeding of hybridized rice During studying carefully, the advantage of New Hybrid Rice Combination and the identification of Comprehensive Traits have to certain grain weight structure identification colony, Its small areas production of hybrid seeds is an essential program.Current breeders are to take people during the small area production of hybrid seeds Work hybridizes the method for bucket, i.e., each female parent takes 4-6 plants to transplant into bucket, carries out the carefully and neatly done strain of people, removes the grain of rice bloomed, or cut Grain husk allows it to expose column cap, the template then manually pasted(Or brown paper)Hybridization bag for packing fruits on carries out mark.Finally will hybridization Bucket is screwed onto beside male parent, is taken powder to inseminate when blooming, has been awarded powder and sealed up sack, prevents foreign pollen string powder from causing to mix.My god Gas will continuously pollinate more than three days when fine, and also to open sealing ventilation in due course reduces humidity in bag.Needed after pollination In time by maternal transplanting in bucket to the stringent ground of isolation condition, harvested in due course after waiting maturation.This method is more original, and program is numerous Miscellaneous, process is more, time consuming expensive, extremely inefficient, and the seed amount obtained is few, with low quality, easily by insect bite, Yi Fa Mould, germination percentage is low, and germination is irregular, and planting percent is low, the accuracy of advantage and Identification of Comprehensive Characters to New Hybrid Rice Combination Influence big.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of small area production of hybrid seeds of hybrid rice with the different female Combination nova of father Method, this method can help breeder easily to produce enough hybrid rice seed structure identification colonies, it is ensured that the standard of identification True property.
In order to solve the above technical problems, patent of the present invention is a kind of small area production of hybrid seeds of hybrid rice with the different female Combination nova of father Method, comprises the following steps:
(1) the good field of isolation condition is selected to implement the small area production of hybrid seeds;
(2) according to the time of infertility of normal Parent, determine that Parent broadcasts difference, it is 20-40 days to broadcast difference;
(3) Parent uses water-raised seedling, the sowing of three phases of male parent point, and a female parent point phase is sowed, and first phase male parent is broadcast with female parent Difference is 40 days, and it is 30 days that second phase male parent broadcasts difference with female parent, and it is 20 days that third phase male parent broadcasts difference with female parent, and female parent is than second phase father Sow within this slow 30 days;
(4) parental and line space, of the right age transplanting, parental 2 are determined:10, male parent inserts 2 rows, and female parent inserts 10 rows, Spacing in the rows is 20cm between male parent line-spacing, male parent spacing in the rows, Parent line-spacing, different female parents, and maternal line-spacing is 15cm, in a phase male parent Three phase male parents are implemented to transplant at the same time during 40 days length of time rice seedlings grow, and using interpolation technique, first phase male parent plants 2*2=4, and second phase male parent is planted 2*2=4 are inserted, third phase male parent plants 2*2=4, and female parent implements transplanting generally at 25 days length of time rice seedlings grow, each female parent material is planted Insert 1 row 10;
(5) when second phase male parent is i.e. by cut, Parent sprays " 920 " at the same time, and dosage is 10-15 grams/acre, is raised daily in male parent Human assistance catches up with powder 2-3 times during flower;
(6) male parent is cut off after all maternal flowerings and cleared up from field, treat maternal ripe time-division kind harvest.
The step(2)Described in broadcast difference be 30 days.
The step(5)Described in Parent spray at the same time " 920 ", dosage be 15 grams/acre.
A kind of above-mentioned hybrid rice, generally per acre can the production of hybrid seeds 1500 with the small area producing method for seed of the different female Combination nova of father Left and right hybrid rice new combination, specific area is depending on the quantity of parental varieties, conventional method of the work efficiency than the prior art Tens times of raising, not only synergy during abridged edition section, and also the seed quality harvested is high, and quantity foot, can fully meet hybrid rice The advantage of Combination nova and the appraisal of Comprehensive Traits are actually needed.
Brief description of the drawings
Fig. 1 is a kind of plantation schematic diagram of the small area production of hybrid seeds of the hybrid rice of the present invention with the different female Combination nova of father.
Embodiment
A kind of hybrid rice comprises the following steps with the small area producing method for seed of the different female Combination nova of father:
(1) the preferable field of selection isolation condition implements the small area production of hybrid seeds as needed;It can so exclude to be subject to foreign pollen Influence, so that it is guaranteed that the seed purity of production of hybrid seeds Combination nova.
(2) according to the time of infertility of normal Parent, determine that Parent broadcasts difference, it is 20-40 days to broadcast difference;Determine Parent just It is 30 days often to broadcast difference, so may insure that the Parent florescence meets substantially, and the exclusion florescence does not meet situation seriously;
(3) Parent uses water-raised seedling, the sowing of three phases of male parent point, and a female parent point phase is sowed, and first phase male parent is broadcast with female parent Difference is 40 days, and it is 30 days that second phase male parent broadcasts difference with female parent, and it is 20 days that third phase male parent broadcasts difference with female parent, and female parent is than second phase father Sow within this slow 30 days, so may insure that all maternal heading flowering periods are included in the heading flowering period of three phase male parents, It may insure Parent flower synchronization;
(4) parental and line space, of the right age transplanting, parental 2 are determined:10, i.e. male parent inserts 2 rows, and female parent inserts 10 OK, spacing in the rows is 20cm between male parent line-spacing, male parent spacing in the rows, Parent line-spacing, different female parents, and maternal line-spacing is 15cm, in a phase Three phase male parents are implemented to transplant at the same time during 40 days male parent length of time rice seedlings grow, and using interpolation technique, i.e. first phase male parent plants 2*2=4, the second phase Male parent plants 2*2=4, and third phase male parent plants 2*2=4, and female parent implements transplanting generally at 25 days length of time rice seedlings grow, each is maternal Material plants 1 row 10;It is 2 to determine parental:10 foundation is formulated with reference to the mode of Production of Large Fields, 2 rows The enough 10 row female parent pollination demands of paternal pollen amount;Male parent line-spacing and spacing in the rows are 20cm, maternal line-spacing 15cm, and spacing in the rows is 20cm, can ensure differentiation of the Parent between the differentiation in field and different female parents;Three phases at 40 days male parent length of time rice seedlings grow phase Male parent is implemented to transplant at the same time, can take into account the three phase male parents growth growth of cereal crop seedlings, dart transfer;Three phase male parents are to ensure often using interpolation technique One phase male parent can abut female parent, be conducive to pollinate.
(5) when second phase male parent is i.e. by cut, Parent sprays " 920 " at the same time, and dosage is 10-15 grams/acre, daily in father Human assistance catches up with powder 2-3 times during this flowering, and " 920 " are gibberellin;Since the Parent of the prior art production hybrid paddy rice production of hybrid seeds is broadcast Difference at 30 days, broadcast difference and equally also concentrate on 30 days by the Parent of most of small production of hybrid seeds material, thus spray " 920 " using the second phase as It is main;The reason for " 920 " dosage is set to 15 grams/acre is that normal male parent material is more sensitive to " 920 ", and 15 grams are enough to promote its spike of rice Cob is extended from neck, and female parent material is with respect to insensitiveness, and general material, which needs 20-30 grams, can still promote it to be needed from neck, some materials Want 50 grams or so could from neck, so 15 grams can promote spike of rice cob moderately elongation without that from neck, will not be fallen in the ripe later stage Volt, is conducive to harvest;It is in order to which the pollen of male parent is fully drifted out that in male parent flowering, human assistance, which catches up with powder 2-3 times, daily Come, be conducive to improve maternal rate of fertilization, so as to improve setting percentage.
(6) male parent is cut off after all maternal flowerings and cleared up from field, treat maternal ripe time-division kind harvest. Cutting off and clear up to not only improve from field male parent improves field permeability, and conveying can be concentrated by field nutrient to female parent by having, and be carried High maternal filling rate, increases grain weight.
Above-mentioned hybrid rice, generally per acre can the production of hybrid seeds 1500 or so with the small area producing method for seed of the different female Combination nova of father Hybrid rice new combination, for specific area depending on the quantity of parental varieties, work efficiency improves tens than current conventional method Times, not only synergy during abridged edition section, and the seed quality harvested is high, and quantity foot, can fully meet New Hybrid Rice Combination Advantage and the appraisal of Comprehensive Traits are actually needed.

Claims (3)

1. a kind of hybrid rice comprises the following steps with the small area producing method for seed of the different female Combination nova of father:
(1) the good field of isolation condition is selected to implement the small area production of hybrid seeds;
(2) according to the time of infertility of normal Parent, determine that Parent broadcasts difference, it is 20-40 days to broadcast difference;
(3) Parent uses water-raised seedling, the sowing of three phases of male parent point, and a female parent point phase is sowed, and first phase male parent is broadcast with female parent Difference is 40 days, and it is 30 days that second phase male parent broadcasts difference with female parent, and it is 20 days that third phase male parent broadcasts difference with female parent, and female parent is than second phase father Sow within this slow 30 days;
(4) parental and line space, of the right age transplanting, parental 2 are determined:10, male parent inserts 2 rows, and female parent inserts 10 rows, Spacing in the rows is 20cm between male parent line-spacing, male parent spacing in the rows, Parent line-spacing, different female parents, and maternal line-spacing is 15cm, in a phase male parent Three phase male parents are implemented to transplant at the same time during 40 days length of time rice seedlings grow, and using interpolation technique, first phase male parent plants 2*2=4, and second phase male parent is planted 2*2=4 are inserted, third phase male parent plants 2*2=4, and female parent implements transplanting generally at 25 days length of time rice seedlings grow, each female parent material is planted Insert 1 row 10;
(5) when second phase male parent is i.e. by cut, Parent sprays " 920 " at the same time, and dosage is 10-15 grams/acre, is raised daily in male parent Human assistance catches up with powder 2-3 times during flower;
(6) male parent is cut off after all maternal flowerings and cleared up from field, treat maternal ripe time-division kind harvest.
2. a kind of hybrid rice according to claim 1 is the same as the small area producing method for seed of the different female Combination nova of father, the step (2)Described in broadcast difference be 30 days.
3. a kind of hybrid rice according to claim 1 is the same as the small area producing method for seed of the different female Combination nova of father, the step (5)Described in Parent spray at the same time " 920 ", dosage be 15 grams/acre.
CN201711298194.8A 2017-12-08 2017-12-08 A kind of small area producing method for seed of hybrid rice with the different female Combination nova of father Pending CN107950383A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109673506A (en) * 2019-02-23 2019-04-26 湖南杂交水稻研究中心 A kind of method of a large amount of preparing hybrid Rice Combinations

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101697708A (en) * 2009-11-27 2010-04-28 天津天隆农业科技有限公司 Method for producing seeds of japonica hybrid rice
CN103931448A (en) * 2014-03-14 2014-07-23 中国水稻研究所 Hybrid rice seed production male parent and female parent same-period mechanical rice transplanting method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101697708A (en) * 2009-11-27 2010-04-28 天津天隆农业科技有限公司 Method for producing seeds of japonica hybrid rice
CN103931448A (en) * 2014-03-14 2014-07-23 中国水稻研究所 Hybrid rice seed production male parent and female parent same-period mechanical rice transplanting method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109673506A (en) * 2019-02-23 2019-04-26 湖南杂交水稻研究中心 A kind of method of a large amount of preparing hybrid Rice Combinations

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