CN107929806B

CN107929806B - Preparation method of artificial skin special for burns

Info

Publication number: CN107929806B
Application number: CN201711374432.9A
Authority: CN
Inventors: 范娟; 陈加宏; 陈兴权; 许博伟; 张淼
Original assignee: Individual
Current assignee: Fan Juan
Priority date: 2017-12-19
Filing date: 2017-12-19
Publication date: 2021-01-12
Anticipated expiration: 2037-12-19
Also published as: CN107929806A

Abstract

The invention discloses a preparation method of artificial skin special for burns, and belongs to the technical field of preparation of medical materials. Mixing chitosan and water for swelling, performing enzymolysis to prepare chitosan enzymolysis liquid, wetting polyethylene glycol and bacterial cellulose by using glycerol, dispersing the mixture in a dilute acetic acid solution to prepare a mixed dispersion liquid, and then shearing the chitosan enzymolysis liquid, the mixed dispersion liquid, onion juice, carbomer dispersion liquid, nano silver powder, sesame oil, plant essential oil, a biomass surfactant, pretreated sepiolite and an auxiliary agent at a high speed to prepare a film-forming emulsion; and marking the two surfaces of the lower epidermis of the plant leaf, spraying a dopamine solution on one surface of the lower epidermis, standing, coating a film-forming emulsion, drying at a low temperature to reach a specified water content, spraying a metal salt solution, standing at a constant temperature and humidity, sterilizing, drying and packaging to obtain the artificial skin special for burns. The artificial skin special for burns obtained by the invention has excellent healing effect and sterilization effect.

Description

Preparation method of artificial skin special for burns

Technical Field

The invention discloses a preparation method of artificial skin special for burns, and belongs to the technical field of preparation of medical materials.

Background

The artificial skin is a skin substitute artificially developed in vitro by using the principles and methods of engineering and cell biology, is used for repairing and replacing defective skin tissues, and can be divided into simple artificial dermis and active composite dermis with epidermal cell layer according to different components.

The skin can not only protect people from being invaded by dirt or bacteria, but also keep the water in the human body from escaping. When a large area of skin is severely burned or damaged, the doctor must immediately introduce fluid and protect the wound, and if only the superficial layer of skin is damaged, new skin will regenerate. If a patient is subjected to a severe burn, the skin cannot be repaired on its own and superficial skin from other parts of the body must usually be transplanted onto the wound. The production of synthetic skin starts with a piece of dermis from which a larger area of skin grows. The skin is one of the important organs of the human body and can be completely renewed within 60 days.

Artificial skin is used for the first treatment of skin grafts. Artificial skin protects wounds from infection and promotes the growth of connective tissue. The immune system of the body gradually breaks down the polymers and the wound heals quickly once the patient's own superficial skin has been implanted.

Whether the artificial skin can be transplanted successfully or not is critical to whether the artificial skin can be vascularized rapidly or not, so that nutrition can be supplied as soon as possible after the artificial skin is transplanted. On one hand, the gene of a factor (VEGF) capable of promoting vascularization is successfully transferred into human fiber cells, so that the human fiber cells can secrete VEGF to promote vascularization; on the other hand, autologous endothelial cells and fibroblasts are implanted into the dermal scaffold through a proper way to induce the formation of new blood vessels, thereby thoroughly solving the problem. At present, the traditional artificial skin still has the problems of poor healing effect and sterilization effect, so research on the traditional artificial skin is needed.

Disclosure of Invention

The invention mainly solves the technical problems that: aiming at the problem that the healing effect and the sterilization effect of the traditional artificial skin are not good, the preparation method of the artificial skin special for burn is provided.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows:

(1) according to the mass ratio of 1: 10-1: 15 mixing chitosan and water, standing for swelling, adding chitosan enzyme accounting for 4-8% of the mass of the chitosan, stirring at constant temperature for enzymolysis, and inactivating enzyme to obtain chitosan enzymolysis liquid;

(2) according to the weight parts, sequentially taking 8-10 parts of polyethylene glycol, 8-10 parts of bacterial cellulose, 0.6-0.8 part of glycerol and 100-200 parts of dilute acetic acid solution, grinding and mixing the polyethylene glycol, the bacterial cellulose and the glycerol, pouring the mixture into the dilute acetic acid solution, stirring and mixing, standing for swelling, heating, stirring and dispersing to obtain a mixed dispersion liquid;

(3) sequentially taking 20-30 parts by weight of chitosan enzymolysis liquid, 20-30 parts by weight of mixed dispersion liquid, 8-10 parts by weight of onion juice, 10-15 parts by weight of carbomer dispersion liquid, 0.3-0.5 part by weight of nano silver powder, 4-8 parts by weight of sesame oil, 3-5 parts by weight of plant essential oil, 0.3-0.5 part by weight of biomass surfactant, 4-8 parts by weight of pretreated sepiolite and 3-5 parts by weight of auxiliary agent, and shearing at high speed to obtain film-forming emulsion;

(4) marking the two surfaces of the lower epidermis of the plant leaf as an A surface and a B surface respectively, spraying a dopamine solution on the B surface, standing for 10-20 min after spraying, then coating a film-forming emulsion, controlling the coating thickness to be 0.8-1.2 mm, drying at low temperature until the water content is 30-40%, then spraying a glutaraldehyde ethanol solution, standing for 3-5 h at constant temperature and humidity, sterilizing, drying, and packaging to obtain the artificial skin special for burn.

The chitosan in the step (1) is chitosan with deacetylation degree of 85-95%.

The preparation process of the carbomer dispersion in the step (3) comprises the following steps: according to the mass ratio of 1: 10-1: 15 mixing carbomer with water, and performing ultrasonic dispersion to obtain carbomer dispersion.

The plant essential oil in the step (3) is any one of lavender essential oil, rose essential oil, camellia essential oil or jasmine essential oil.

The biomass surfactant in the step (3) is prepared from lecithin and tea saponin in a mass ratio of 1: 1-1: 3 is prepared by compounding.

The preparation process of the pretreated sepiolite in the step (3) comprises the following steps: the sepiolite is crushed, sieved, soaked by hydrochloric acid, filtered, dried and roasted to obtain the pretreated sepiolite.

The auxiliary agent in the step (3) is prepared from the following raw materials in parts by weight: 10-20 parts of oleic acid, 8-10 parts of linoleic acid and 4-8 parts of azone.

The plant leaf in the step (4) is any one of oleander leaf, aloe leaf, gladiolus leaf or sansevieria leaf.

The invention has the beneficial effects that:

(1) the technical scheme of the invention is that the lower epidermis of the plant leaf is taken as a product substrate, the surface of the lower epidermis of the plant leaf contains pores required by plant respiration, the pores have good air permeability, living plant cells in the lower epidermis can repeatedly absorb water and prevent water along with the change of ion concentration in water in the surrounding environment, good humidity regulating effect is achieved, a small amount of vitamins and mineral substances in the plant cells can be released in the moisture releasing process, the healing of burn wounds is accelerated, the surface of the lower epidermis is modified by dopamine, a polydopamine composite layer is formed by oxidation and auto-polymerization of the dopamine on the surface of the leaf epidermis, the modification of the leaf epidermis is realized, the adsorption performance of the lower epidermis of the plant leaf on film-forming emulsion is improved, and part of the dopamine can be used as a medicinal component to permeate into the blood of burn skin tissues through the pores on the surface of the lower epidermis of the plant leaf to regulate vasodilation, the symptoms of polar renal failure, shock and the like of a large-area burn patient are avoided;

(2) according to the technical scheme, the auxiliary agent component consisting of oleic acid, linoleic acid and azone is added into the product, and the three components can synergistically dissolve fat-soluble components at the scabbing part of burned skin in the using process, so that the absorption of active components in the product by the skin at the burned part is improved, and the effects of inducing cell growth and promoting tissue healing are achieved, for example, the anti-infection capacity of bacterial cellulose in the product to burned skin tissues is promoted;

(3) according to the technical scheme, carbomer dispersion liquid is added, a large number of carboxylic acid groups are contained in the carbomer molecular structure, nano silver powder in a system can be adsorbed, so that the nano silver powder is well dispersed in the system, the carbomer can also interact with glycoprotein on the surface of cell membranes of histiocytes at burn parts to form physical entanglement, then hydrogen bonds are formed with sugar residues on oligosaccharide chains of the glycoprotein, a strong mucus gel network structure is generated, the nano silver powder is adsorbed and fixed in the network structure, the residence time of the nano silver powder at the burn parts is prolonged, and therefore a good sterilization effect is achieved;

(4) according to the technical scheme, a small amount of onion juice is added, so that on one hand, the onion juice contains volatile matters with strong sterilization and virus resistance, on the other hand, the onion juice contains a trace element selenium, a human body can generate glutathione, the glutathione has the function of conveying oxygen to allow tissue cells of burn parts to breathe, the irritation of the plant source raw materials to the tissues of the burn parts of the human body is small, the pain of a patient after use can be effectively reduced, and the mental recovery of the patient is facilitated.

Detailed Description

Pouring sepiolite into a crusher, crushing, sieving with a 325-500 mesh sieve to obtain sepiolite powder, and mixing the sepiolite powder with 10% hydrochloric acid according to a mass ratio of 1: 8-1: 10, mixing and soaking for 4-6 hours, filtering to obtain a filter cake, drying the obtained filter cake to constant weight at the temperature of 105-110 ℃ to obtain dry sepiolite powder, transferring the obtained dry sepiolite powder into a tubular furnace, roasting for 3-5 hours at the temperature of 260-280 ℃, cooling to room temperature along with the furnace, and discharging to obtain pretreated sepiolite; according to the mass ratio of 1: 10-1: 15, mixing chitosan and water, pouring the mixture into a No. 1 beaker, stirring and mixing the mixture for 10-15 min by using a glass rod, standing and swelling the mixture for 3-5 h at room temperature, adding chitosan enzyme accounting for 4-8% of the mass of the chitosan into the No. 1 beaker after swelling is finished, moving the No. 1 beaker into a digital display speed measurement constant-temperature magnetic stirrer, stirring and performing enzymolysis for 3-5 h at a constant temperature of 35-40 ℃ and a rotating speed of 300-500 r/min, performing heat preservation and enzyme inactivation for 10-20 min at a temperature of 85-95 ℃, cooling and discharging the mixture to obtain a chitosan enzymolysis solution; according to the weight parts, sequentially taking 8-10 parts of polyethylene glycol, 8-10 parts of bacterial cellulose, 0.6-0.8 part of glycerol and 100-200 parts of a dilute acetic acid solution with the mass fraction of 1-5%, firstly pouring the polyethylene glycol, the bacterial cellulose and the glycerol into a mortar, grinding and mixing for 10-20 min, then pouring the ground material into a No. 2 beaker containing the dilute acetic acid solution, stirring and mixing for 10-15 min by using a glass rod, standing and swelling for 3-6 h under the room temperature condition, then moving the beaker into a digital display speed measurement constant temperature magnetic stirrer, and heating, stirring and dispersing for 45-60 min under the conditions that the temperature is 90-95 ℃ and the rotating speed is 400-500 r/min to obtain a mixed dispersion liquid; sequentially taking 20-30 parts by weight of chitosan enzymolysis liquid, 20-30 parts by weight of mixed dispersion liquid, 8-10 parts by weight of onion juice, 10-15 parts by weight of carbomer dispersion liquid, 0.3-0.5 part by weight of nano silver powder, 4-8 parts by weight of sesame oil, 3-5 parts by weight of plant essential oil, 0.3-0.5 part by weight of biomass surfactant and 4-8 parts by weight of plant essential oilAdding pretreated sepiolite and 3-5 parts of auxiliary agent into a high-speed shearing emulsifying machine, shearing at a high speed of 8000-12000 r/min for 10-20 min, and discharging to obtain film-forming emulsion; cutting the lower epidermis of the plant leaf by using a blade, respectively marking the two surfaces of the lower epidermis of the plant leaf as an A surface and a B surface, spraying a dopamine solution with the mass concentration of 2g/L on the B surface, and controlling the spraying amount of the dopamine solution to be 1-3 g/cm²After the dopamine solution is sprayed, standing the lower epidermis of the plant leaf for 10-20 min at room temperature, then coating a film-forming emulsion on the surface B, controlling the coating thickness of the film-forming emulsion to be 0.8-1.2 mm, after the film-forming emulsion is coated, drying the lower epidermis of the plant leaf at low temperature under the condition that the temperature is 35-40 ℃ until the water content is 30-40%, after the drying is finished, spraying a glutaraldehyde ethanol solution with the mass fraction of 8-10%, and controlling the spraying amount of the glutaraldehyde ethanol solution to be 0.1-0.3 mL/cm²After the glutaraldehyde ethanol solution is sprayed, the lower epidermis of the plant leaf is moved into an aseptic greenhouse, the plant leaf is kept stand for 3-5 hours at the constant temperature and humidity under the conditions that the temperature is 35-40 ℃ and the humidity is 45-50%, then ultraviolet sterilization is carried out for 6-8 hours to obtain a sterilization membrane, the obtained sterilization membrane is dried for 4-6 hours at the temperature of 45-50 ℃, and then the dried sterilization membrane is packaged in vacuum to obtain the artificial skin special for burn. The chitosan is chitosan with deacetylation degree of 85-95%. The preparation process of the carbomer dispersion comprises the following steps: according to the mass ratio of 1: 10-1: 15 mixing carbomer with water, and performing ultrasonic dispersion to obtain carbomer dispersion. The plant essential oil is any one of lavender essential oil, rose essential oil, camellia essential oil or jasmine essential oil. The biomass surfactant is prepared from lecithin and tea saponin in a mass ratio of 1: 1-1: 3 is prepared by compounding. The auxiliary agent comprises the following raw materials in parts by weight: 10-20 parts of oleic acid, 8-10 parts of linoleic acid and 4-8 parts of azone. The plant leaf is any one of oleander leaf, aloe leaf, gladiolus leaf or sansevieria leaf.

Example 1

Pouring sepiolite into a grinder, grinding, sieving by a 500-mesh sieve to obtain sepiolite powder, and mixing the sepiolite powder with 10% hydrochloric acid according to a mass ratio of 1: 10 mixing and soaking for 6h, and filtering to obtainDrying the obtained filter cake to constant weight at the temperature of 110 ℃ to obtain dry sepiolite powder, transferring the obtained dry sepiolite powder into a tubular furnace, roasting for 5 hours at the temperature of 280 ℃, cooling to room temperature along with the furnace, and discharging to obtain pretreated sepiolite; according to the mass ratio of 1: 15, mixing chitosan and water, pouring the mixture into a No. 1 beaker, stirring and mixing the mixture for 15min by using a glass rod, standing and swelling the mixture for 5h at room temperature, adding chitosan enzyme accounting for 8 percent of the mass of the chitosan into the No. 1 beaker after the swelling is finished, moving the No. 1 beaker into a digital display speed measurement constant-temperature magnetic stirrer, stirring and performing enzymolysis for 5h at a constant temperature of 40 ℃ and a rotating speed of 500r/min, performing heat preservation and enzyme deactivation for 20min at a temperature of 95 ℃, cooling, and discharging the mixture to obtain chitosan enzymatic hydrolysate; according to the weight parts, sequentially taking 10 parts of polyethylene glycol, 10 parts of bacterial cellulose, 0.8 part of glycerol and 200 parts of a dilute acetic acid solution with the mass fraction of 5%, pouring the polyethylene glycol, the bacterial cellulose and the glycerol into a mortar, grinding and mixing for 20min, pouring the ground material into a No. 2 beaker containing the dilute acetic acid solution, stirring and mixing for 15min by using a glass rod, standing and swelling for 6h at room temperature, moving the beaker into a digital display speed measurement constant-temperature magnetic stirrer, and heating, stirring and dispersing for 60min at the temperature of 95 ℃ and the rotating speed of 500r/min to obtain a mixed dispersion liquid; according to the weight parts, sequentially taking 30 parts of chitosan enzymolysis liquid, 30 parts of mixed dispersion liquid, 10 parts of onion juice, 15 parts of carbomer dispersion liquid, 0.5 part of nano silver powder, 8 parts of sesame oil, 5 parts of vegetable essential oil, 0.5 part of biomass surfactant, 8 parts of pretreated sepiolite and 5 parts of auxiliary agent, adding the mixture into a high-speed shearing emulsifying machine, shearing at a high speed for 20min under the condition that the rotating speed is 12000r/min, and discharging to obtain film-forming emulsion; cutting the lower epidermis of the plant leaf with a blade, marking the two surfaces of the lower epidermis of the plant leaf as an A surface and a B surface respectively, spraying a dopamine solution with the mass concentration of 2g/L on the B surface, and controlling the spraying amount of the dopamine solution to be 3g/cm²Standing the lower epidermis of the plant leaves for 20min at room temperature after finishing the spraying of the dopamine solution, then coating the B surface with film-forming emulsion, controlling the coating thickness of the film-forming emulsion to be 1.2mm, drying the lower epidermis of the plant leaves at low temperature at 40 ℃ until the water content is 40% after the coating of the film-forming emulsion is finished, and dryingSpraying 10% glutaraldehyde ethanol solution to surface B, and controlling spraying amount of the glutaraldehyde ethanol solution to be 0.3mL/cm²After the glutaraldehyde ethanol solution is sprayed, the lower epidermis of the plant leaf is moved into a sterile greenhouse, the plant leaf is kept stand for 5 hours at a constant temperature and humidity under the conditions that the temperature is 40 ℃ and the humidity is 50%, then ultraviolet sterilization is carried out for 8 hours to obtain a sterilization membrane, the obtained sterilization membrane is dried for 6 hours under the condition that the temperature is 50 ℃, and then the dried sterilization membrane is packaged in vacuum to obtain the artificial skin special for burn. The chitosan is chitosan with deacetylation degree of 95%. The preparation process of the carbomer dispersion comprises the following steps: according to the mass ratio of 1: 15 mixing carbomer with water, and performing ultrasonic dispersion to obtain carbomer dispersion. The plant essential oil is lavender essential oil. The biomass surfactant is prepared from lecithin and tea saponin in a mass ratio of 1: 3 is prepared by compounding. The auxiliary agent comprises the following raw materials in parts by weight: 20 parts of oleic acid, 10 parts of linoleic acid and 8 parts of azone. The plant leaves are oleander leaves.

Example 2

Pouring sepiolite into a grinder, grinding, sieving by a 500-mesh sieve to obtain sepiolite powder, and mixing the sepiolite powder with 10% hydrochloric acid according to a mass ratio of 1: 10, mixing and soaking for 6 hours, filtering to obtain a filter cake, drying the obtained filter cake to constant weight at the temperature of 110 ℃ to obtain dry sepiolite powder, transferring the obtained dry sepiolite powder into a tubular furnace, roasting for 5 hours at the temperature of 280 ℃, cooling to room temperature along with the furnace, and discharging to obtain pretreated sepiolite; according to the weight parts, sequentially taking 10 parts of polyethylene glycol, 10 parts of bacterial cellulose, 0.8 part of glycerol and 200 parts of a dilute acetic acid solution with the mass fraction of 5%, pouring the polyethylene glycol, the bacterial cellulose and the glycerol into a mortar, grinding and mixing for 20min, pouring the ground material into a No. 2 beaker containing the dilute acetic acid solution, stirring and mixing for 15min by using a glass rod, standing and swelling for 6h at room temperature, moving the beaker into a digital display speed measurement constant-temperature magnetic stirrer, and heating, stirring and dispersing for 60min at the temperature of 95 ℃ and the rotating speed of 500r/min to obtain a mixed dispersion liquid; according to the weight portion, 30 portions of chitosan, 30 portions of mixed dispersion, 10 portions of onion juice, 15 portions of carbomer dispersion, 0.5 portion of nano silver powder, 8 portions of sesame oil and 5 portions ofAdding plant essential oil, 0.5 part of biomass surfactant, 8 parts of pretreated sepiolite and 5 parts of auxiliary agent into a high-speed shearing emulsifying machine, shearing at a high speed for 20min under the condition that the rotating speed is 12000r/min, and discharging to obtain film-forming emulsion; cutting the lower epidermis of the plant leaf with a blade, marking the two surfaces of the lower epidermis of the plant leaf as an A surface and a B surface respectively, spraying a dopamine solution with the mass concentration of 2g/L on the B surface, and controlling the spraying amount of the dopamine solution to be 3g/cm²After the dopamine solution is sprayed, standing the lower epidermis of the plant leaf at room temperature for 20min, then coating a film-forming emulsion on the surface B, controlling the coating thickness of the film-forming emulsion to be 1.2mm, after the film-forming emulsion is coated, drying the lower epidermis of the plant leaf at a low temperature of 40 ℃ until the water content is 40%, after the drying is finished, spraying a glutaraldehyde ethanol solution with the mass fraction of 10% on the surface B, and controlling the spraying amount of the glutaraldehyde ethanol solution to be 0.3mL/cm²After the glutaraldehyde ethanol solution is sprayed, the lower epidermis of the plant leaf is moved into a sterile greenhouse, the plant leaf is kept stand for 5 hours at a constant temperature and humidity under the conditions that the temperature is 40 ℃ and the humidity is 50%, then ultraviolet sterilization is carried out for 8 hours to obtain a sterilization membrane, the obtained sterilization membrane is dried for 6 hours under the condition that the temperature is 50 ℃, and then the dried sterilization membrane is packaged in vacuum to obtain the artificial skin special for burn. The chitosan is chitosan with deacetylation degree of 95%. The preparation process of the carbomer dispersion comprises the following steps: according to the mass ratio of 1: 15 mixing carbomer with water, and performing ultrasonic dispersion to obtain carbomer dispersion. The plant essential oil is lavender essential oil. The biomass surfactant is prepared from lecithin and tea saponin in a mass ratio of 1: 3 is prepared by compounding. The auxiliary agent comprises the following raw materials in parts by weight: 20 parts of oleic acid, 10 parts of linoleic acid and 8 parts of azone. The plant leaves are oleander leaves.

Example 3

Pouring sepiolite into a grinder, grinding, sieving by a 500-mesh sieve to obtain sepiolite powder, and mixing the sepiolite powder with 10% hydrochloric acid according to a mass ratio of 1: 10 mixing and soaking for 6h, filtering to obtain filter cake, drying the filter cake to constant weight at 110 deg.C to obtain dried sepiolite powder, transferring the dried sepiolite powder into a tubular furnace, roasting at 280 deg.C for 5h, and cooling with the furnaceCooling to room temperature, and discharging to obtain pretreated sepiolite; according to the mass ratio of 1: 15, mixing chitosan and water, pouring the mixture into a No. 1 beaker, stirring and mixing the mixture for 15min by using a glass rod, standing and swelling the mixture for 5h at room temperature, adding chitosan enzyme accounting for 8 percent of the mass of the chitosan into the No. 1 beaker after the swelling is finished, moving the No. 1 beaker into a digital display speed measurement constant-temperature magnetic stirrer, stirring and performing enzymolysis for 5h at a constant temperature of 40 ℃ and a rotating speed of 500r/min, performing heat preservation and enzyme deactivation for 20min at a temperature of 95 ℃, cooling, and discharging the mixture to obtain chitosan enzymatic hydrolysate; according to the weight parts, 10 parts of polyethylene glycol, 0.8 part of glycerol and 200 parts of dilute acetic acid solution with the mass fraction of 5% are sequentially taken, the polyethylene glycol and the glycerol are poured into a mortar, ground and mixed for 20min, then the ground material is poured into a No. 2 beaker containing the dilute acetic acid solution, stirred and mixed for 15min by a glass rod, then kept stand and swelled for 6h at room temperature, then the beaker is moved into a digital display speed measurement constant temperature magnetic stirrer, and heated, stirred and dispersed for 60min at the temperature of 95 ℃ and the rotating speed of 500r/min to obtain mixed dispersion liquid; according to the weight parts, sequentially taking 30 parts of chitosan enzymolysis liquid, 30 parts of mixed dispersion liquid, 10 parts of onion juice, 15 parts of carbomer dispersion liquid, 0.5 part of nano silver powder, 8 parts of sesame oil, 5 parts of vegetable essential oil, 0.5 part of biomass surfactant, 8 parts of pretreated sepiolite and 5 parts of auxiliary agent, adding the mixture into a high-speed shearing emulsifying machine, shearing at a high speed for 20min under the condition that the rotating speed is 12000r/min, and discharging to obtain film-forming emulsion; cutting the lower epidermis of the plant leaf with a blade, marking the two surfaces of the lower epidermis of the plant leaf as an A surface and a B surface respectively, spraying a dopamine solution with the mass concentration of 2g/L on the B surface, and controlling the spraying amount of the dopamine solution to be 3g/cm²After the dopamine solution is sprayed, standing the lower epidermis of the plant leaf at room temperature for 20min, then coating a film-forming emulsion on the surface B, controlling the coating thickness of the film-forming emulsion to be 1.2mm, after the film-forming emulsion is coated, drying the lower epidermis of the plant leaf at a low temperature of 40 ℃ until the water content is 40%, after the drying is finished, spraying a glutaraldehyde ethanol solution with the mass fraction of 10% on the surface B, and controlling the spraying amount of the glutaraldehyde ethanol solution to be 0.3mL/cm²After the spraying of the glutaraldehyde ethanol solution is finished, the lower epidermis of the plant leaf is moved into a sterile greenhouse at the temperature of 40 ℃,standing at constant temperature and humidity for 5h under the condition that the humidity is 50%, performing ultraviolet sterilization for 8h to obtain a sterilization membrane, drying the sterilization membrane at the temperature of 50 ℃ for 6h, and performing vacuum packaging on the dried sterilization membrane to obtain the artificial skin special for burns. The chitosan is chitosan with deacetylation degree of 95%. The preparation process of the carbomer dispersion comprises the following steps: according to the mass ratio of 1: 15 mixing carbomer with water, and performing ultrasonic dispersion to obtain carbomer dispersion. The plant essential oil is lavender essential oil. The biomass surfactant is prepared from lecithin and tea saponin in a mass ratio of 1: 3 is prepared by compounding. The auxiliary agent comprises the following raw materials in parts by weight: 20 parts of oleic acid, 10 parts of linoleic acid and 8 parts of azone. The plant leaves are oleander leaves.

Example 4

Pouring sepiolite into a grinder, grinding, sieving by a 500-mesh sieve to obtain sepiolite powder, and mixing the sepiolite powder with 10% hydrochloric acid according to a mass ratio of 1: 10, mixing and soaking for 6 hours, filtering to obtain a filter cake, drying the obtained filter cake to constant weight at the temperature of 110 ℃ to obtain dry sepiolite powder, transferring the obtained dry sepiolite powder into a tubular furnace, roasting for 5 hours at the temperature of 280 ℃, cooling to room temperature along with the furnace, and discharging to obtain pretreated sepiolite; according to the mass ratio of 1: 15, mixing chitosan and water, pouring the mixture into a No. 1 beaker, stirring and mixing the mixture for 15min by using a glass rod, standing and swelling the mixture for 5h at room temperature, adding chitosan enzyme accounting for 8 percent of the mass of the chitosan into the No. 1 beaker after the swelling is finished, moving the No. 1 beaker into a digital display speed measurement constant-temperature magnetic stirrer, stirring and performing enzymolysis for 5h at a constant temperature of 40 ℃ and a rotating speed of 500r/min, performing heat preservation and enzyme deactivation for 20min at a temperature of 95 ℃, cooling, and discharging the mixture to obtain chitosan enzymatic hydrolysate; according to the weight portion, 10 portions of polyethylene glycol, 10 portions of bacterial cellulose, 0.8 portion of glycerol and 200 portions of dilute acetic acid solution with the mass fraction of 5% are taken in sequence, the polyethylene glycol, the bacterial cellulose and the glycerol are poured into a mortar, ground and mixed for 20min, then the ground material is poured into a No. 2 beaker containing the dilute acetic acid solution, stirred and mixed for 15min by a glass rod, kept stand and swelled for 6h under the condition of room temperature, then the beaker is moved into a digital display speed measurement constant temperature magnetic stirrer, and the temperature and the rotating speed are 95 ℃ and 500r/minHeating, stirring and dispersing for 60min to obtain a mixed dispersion liquid; according to the weight parts, sequentially taking 30 parts of chitosan enzymolysis liquid, 30 parts of mixed dispersion liquid, 15 parts of carbomer dispersion liquid, 0.5 part of nano silver powder, 8 parts of sesame oil, 5 parts of vegetable essential oil, 0.5 part of biomass surfactant, 8 parts of pretreated sepiolite and 5 parts of auxiliary agent, adding the mixture into a high-speed shearing emulsifying machine, shearing the mixture at a high speed for 20min under the condition that the rotating speed is 12000r/min, and discharging the mixture to obtain film-forming emulsion; cutting the lower epidermis of the plant leaf with a blade, marking the two surfaces of the lower epidermis of the plant leaf as an A surface and a B surface respectively, spraying a dopamine solution with the mass concentration of 2g/L on the B surface, and controlling the spraying amount of the dopamine solution to be 3g/cm²After the dopamine solution is sprayed, standing the lower epidermis of the plant leaf at room temperature for 20min, then coating a film-forming emulsion on the surface B, controlling the coating thickness of the film-forming emulsion to be 1.2mm, after the film-forming emulsion is coated, drying the lower epidermis of the plant leaf at a low temperature of 40 ℃ until the water content is 40%, after the drying is finished, spraying a glutaraldehyde ethanol solution with the mass fraction of 10% on the surface B, and controlling the spraying amount of the glutaraldehyde ethanol solution to be 0.3mL/cm²After the glutaraldehyde ethanol solution is sprayed, the lower epidermis of the plant leaf is moved into a sterile greenhouse, the plant leaf is kept stand for 5 hours at a constant temperature and humidity under the conditions that the temperature is 40 ℃ and the humidity is 50%, then ultraviolet sterilization is carried out for 8 hours to obtain a sterilization membrane, the obtained sterilization membrane is dried for 6 hours under the condition that the temperature is 50 ℃, and then the dried sterilization membrane is packaged in vacuum to obtain the artificial skin special for burn. The chitosan is chitosan with deacetylation degree of 95%. The preparation process of the carbomer dispersion comprises the following steps: according to the mass ratio of 1: 15 mixing carbomer with water, and performing ultrasonic dispersion to obtain carbomer dispersion. The plant essential oil is lavender essential oil. The biomass surfactant is prepared from lecithin and tea saponin in a mass ratio of 1: 3 is prepared by compounding. The auxiliary agent comprises the following raw materials in parts by weight: 20 parts of oleic acid, 10 parts of linoleic acid and 8 parts of azone. The plant leaves are oleander leaves.

Example 5

Pouring sepiolite into a grinder, grinding, sieving by a 500-mesh sieve to obtain sepiolite powder, and mixing the sepiolite powder with 10% hydrochloric acid according to a mass ratio of 1: 10 mixing and soaking for 6h, and filteringFiltering to obtain a filter cake, drying the obtained filter cake to constant weight at the temperature of 110 ℃ to obtain dry sepiolite powder, transferring the obtained dry sepiolite powder into a tubular furnace, roasting for 5 hours at the temperature of 280 ℃, cooling to room temperature along with the furnace, and discharging to obtain pretreated sepiolite; according to the mass ratio of 1: 15, mixing chitosan and water, pouring the mixture into a No. 1 beaker, stirring and mixing the mixture for 15min by using a glass rod, standing and swelling the mixture for 5h at room temperature, adding chitosan enzyme accounting for 8 percent of the mass of the chitosan into the No. 1 beaker after the swelling is finished, moving the No. 1 beaker into a digital display speed measurement constant-temperature magnetic stirrer, stirring and performing enzymolysis for 5h at a constant temperature of 40 ℃ and a rotating speed of 500r/min, performing heat preservation and enzyme deactivation for 20min at a temperature of 95 ℃, cooling, and discharging the mixture to obtain chitosan enzymatic hydrolysate; according to the weight parts, sequentially taking 10 parts of polyethylene glycol, 10 parts of bacterial cellulose, 0.8 part of glycerol and 200 parts of a dilute acetic acid solution with the mass fraction of 5%, pouring the polyethylene glycol, the bacterial cellulose and the glycerol into a mortar, grinding and mixing for 20min, pouring the ground material into a No. 2 beaker containing the dilute acetic acid solution, stirring and mixing for 15min by using a glass rod, standing and swelling for 6h at room temperature, moving the beaker into a digital display speed measurement constant-temperature magnetic stirrer, and heating, stirring and dispersing for 60min at the temperature of 95 ℃ and the rotating speed of 500r/min to obtain a mixed dispersion liquid; according to the weight parts, sequentially taking 30 parts of chitosan enzymolysis liquid, 30 parts of mixed dispersion liquid, 10 parts of onion juice, 0.5 part of nano silver powder, 8 parts of sesame oil, 5 parts of vegetable essential oil, 0.5 part of biomass surfactant, 8 parts of pretreated sepiolite and 5 parts of auxiliary agent, adding the mixture into a high-speed shearing emulsifying machine, shearing the mixture at a high speed for 20min under the condition that the rotating speed is 12000r/min, and discharging the mixture to obtain film-forming emulsion; cutting the lower epidermis of the plant leaf with a blade, marking the two surfaces of the lower epidermis of the plant leaf as an A surface and a B surface respectively, spraying a dopamine solution with the mass concentration of 2g/L on the B surface, and controlling the spraying amount of the dopamine solution to be 3g/cm²After the dopamine solution is sprayed, standing the lower epidermis of the plant leaves at room temperature for 20min, then coating the B surface with film-forming emulsion, controlling the coating thickness of the film-forming emulsion to be 1.2mm, after the film-forming emulsion is coated, drying the lower epidermis of the plant leaves at a low temperature of 40 ℃ until the water content is 40%, and after the drying is finished, spraying the B surface with the film-forming emulsionCoating a glutaraldehyde ethanol solution with the mass fraction of 10%, and controlling the spraying amount of the glutaraldehyde ethanol solution to be 0.3mL/cm²After the glutaraldehyde ethanol solution is sprayed, the lower epidermis of the plant leaf is moved into a sterile greenhouse, the plant leaf is kept stand for 5 hours at a constant temperature and humidity under the conditions that the temperature is 40 ℃ and the humidity is 50%, then ultraviolet sterilization is carried out for 8 hours to obtain a sterilization membrane, the obtained sterilization membrane is dried for 6 hours under the condition that the temperature is 50 ℃, and then the dried sterilization membrane is packaged in vacuum to obtain the artificial skin special for burn. The chitosan is chitosan with deacetylation degree of 95%. The plant essential oil is lavender essential oil. The biomass surfactant is prepared from lecithin and tea saponin in a mass ratio of 1: 3 is prepared by compounding. The auxiliary agent comprises the following raw materials in parts by weight: 20 parts of oleic acid, 10 parts of linoleic acid and 8 parts of azone. The plant leaves are oleander leaves.

Example 6

Pouring sepiolite into a grinder, grinding, sieving by a 500-mesh sieve to obtain sepiolite powder, and mixing the sepiolite powder with 10% hydrochloric acid according to a mass ratio of 1: 10, mixing and soaking for 6 hours, filtering to obtain a filter cake, drying the obtained filter cake to constant weight at the temperature of 110 ℃ to obtain dry sepiolite powder, transferring the obtained dry sepiolite powder into a tubular furnace, roasting for 5 hours at the temperature of 280 ℃, cooling to room temperature along with the furnace, and discharging to obtain pretreated sepiolite; according to the mass ratio of 1: 15, mixing chitosan and water, pouring the mixture into a No. 1 beaker, stirring and mixing the mixture for 15min by using a glass rod, standing and swelling the mixture for 5h at room temperature, adding chitosan enzyme accounting for 8 percent of the mass of the chitosan into the No. 1 beaker after the swelling is finished, moving the No. 1 beaker into a digital display speed measurement constant-temperature magnetic stirrer, stirring and performing enzymolysis for 5h at a constant temperature of 40 ℃ and a rotating speed of 500r/min, performing heat preservation and enzyme deactivation for 20min at a temperature of 95 ℃, cooling, and discharging the mixture to obtain chitosan enzymatic hydrolysate; according to the weight portion, 10 portions of polyethylene glycol, 10 portions of bacterial cellulose, 0.8 portion of glycerol and 200 portions of dilute acetic acid solution with the mass fraction of 5% are taken in sequence, the polyethylene glycol, the bacterial cellulose and the glycerol are poured into a mortar, ground and mixed for 20min, the ground material is poured into a No. 2 beaker containing the dilute acetic acid solution, stirred and mixed for 15min by a glass rod, kept stand and swelled for 6h at room temperature, and then the beaker is moved intoA digital display speed measurement constant temperature magnetic stirrer is heated, stirred and dispersed for 60min under the conditions that the temperature is 95 ℃ and the rotating speed is 500r/min to obtain a mixed dispersion liquid; according to the weight parts, sequentially taking 30 parts of chitosan enzymolysis liquid, 30 parts of mixed dispersion liquid, 10 parts of onion juice, 15 parts of carbomer dispersion liquid, 0.5 part of nano silver powder, 5 parts of vegetable essential oil, 0.5 part of biomass surfactant, 8 parts of pretreated sepiolite and 5 parts of auxiliary agent, adding the mixture into a high-speed shearing emulsifying machine, shearing the mixture at a high speed for 20min under the condition that the rotating speed is 12000r/min, and discharging the mixture to obtain film-forming emulsion; cutting the lower epidermis of the plant leaf with a blade, marking the two surfaces of the lower epidermis of the plant leaf as an A surface and a B surface respectively, spraying a dopamine solution with the mass concentration of 2g/L on the B surface, and controlling the spraying amount of the dopamine solution to be 3g/cm²After the dopamine solution is sprayed, standing the lower epidermis of the plant leaf at room temperature for 20min, then coating a film-forming emulsion on the surface B, controlling the coating thickness of the film-forming emulsion to be 1.2mm, after the film-forming emulsion is coated, drying the lower epidermis of the plant leaf at a low temperature of 40 ℃ until the water content is 40%, after the drying is finished, spraying a glutaraldehyde ethanol solution with the mass fraction of 10% on the surface B, and controlling the spraying amount of the glutaraldehyde ethanol solution to be 0.3mL/cm²After the glutaraldehyde ethanol solution is sprayed, the lower epidermis of the plant leaf is moved into a sterile greenhouse, the plant leaf is kept stand for 5 hours at a constant temperature and humidity under the conditions that the temperature is 40 ℃ and the humidity is 50%, then ultraviolet sterilization is carried out for 8 hours to obtain a sterilization membrane, the obtained sterilization membrane is dried for 6 hours under the condition that the temperature is 50 ℃, and then the dried sterilization membrane is packaged in vacuum to obtain the artificial skin special for burn. The chitosan is chitosan with deacetylation degree of 95%. The preparation process of the carbomer dispersion comprises the following steps: according to the mass ratio of 1: 15 mixing carbomer with water, and performing ultrasonic dispersion to obtain carbomer dispersion. The plant essential oil is lavender essential oil. The biomass surfactant is prepared from lecithin and tea saponin in a mass ratio of 1: 3 is prepared by compounding. The auxiliary agent comprises the following raw materials in parts by weight: 20 parts of oleic acid, 10 parts of linoleic acid and 8 parts of azone. The plant leaves are oleander leaves.

Example 7

Pouring sepiolite into a pulverizer, pulverizing, sieving with 500 mesh sieve to obtain sepiolite powder, and sievingSepiolite powder and hydrochloric acid with the mass fraction of 10 percent are mixed according to the mass ratio of 1: 10, mixing and soaking for 6 hours, filtering to obtain a filter cake, drying the obtained filter cake to constant weight at the temperature of 110 ℃ to obtain dry sepiolite powder, transferring the obtained dry sepiolite powder into a tubular furnace, roasting for 5 hours at the temperature of 280 ℃, cooling to room temperature along with the furnace, and discharging to obtain pretreated sepiolite; according to the mass ratio of 1: 15, mixing chitosan and water, pouring the mixture into a No. 1 beaker, stirring and mixing the mixture for 15min by using a glass rod, standing and swelling the mixture for 5h at room temperature, adding chitosan enzyme accounting for 8 percent of the mass of the chitosan into the No. 1 beaker after the swelling is finished, moving the No. 1 beaker into a digital display speed measurement constant-temperature magnetic stirrer, stirring and performing enzymolysis for 5h at a constant temperature of 40 ℃ and a rotating speed of 500r/min, performing heat preservation and enzyme deactivation for 20min at a temperature of 95 ℃, cooling, and discharging the mixture to obtain chitosan enzymatic hydrolysate; according to the weight parts, sequentially taking 10 parts of polyethylene glycol, 10 parts of bacterial cellulose, 0.8 part of glycerol and 200 parts of a dilute acetic acid solution with the mass fraction of 5%, pouring the polyethylene glycol, the bacterial cellulose and the glycerol into a mortar, grinding and mixing for 20min, pouring the ground material into a No. 2 beaker containing the dilute acetic acid solution, stirring and mixing for 15min by using a glass rod, standing and swelling for 6h at room temperature, moving the beaker into a digital display speed measurement constant-temperature magnetic stirrer, and heating, stirring and dispersing for 60min at the temperature of 95 ℃ and the rotating speed of 500r/min to obtain a mixed dispersion liquid; according to the weight parts, sequentially taking 30 parts of chitosan enzymolysis liquid, 30 parts of mixed dispersion liquid, 10 parts of onion juice, 15 parts of carbomer dispersion liquid, 0.5 part of nano silver powder, 8 parts of sesame oil, 0.5 part of biomass surfactant, 8 parts of pretreated sepiolite and 5 parts of auxiliary agent, adding the mixture into a high-speed shearing emulsifying machine, shearing the mixture at a high speed for 20min under the condition that the rotating speed is 12000r/min, and discharging the mixture to obtain film-forming emulsion; cutting the lower epidermis of the plant leaf with a blade, marking the two surfaces of the lower epidermis of the plant leaf as an A surface and a B surface respectively, spraying a dopamine solution with the mass concentration of 2g/L on the B surface, and controlling the spraying amount of the dopamine solution to be 3g/cm²After the dopamine solution is sprayed, standing the lower epidermis of the plant leaves for 20min at room temperature, then coating the B surface with film-forming emulsion, controlling the coating thickness of the film-forming emulsion to be 1.2mm, and after the film-forming emulsion is coated, heating the lower epidermis of the plant leaves to be warmDrying at 40 deg.C to water content of 40%, spraying 10% glutaraldehyde ethanol solution onto surface B after drying, and controlling spraying amount of glutaraldehyde ethanol solution to be 0.3mL/cm²After the glutaraldehyde ethanol solution is sprayed, the lower epidermis of the plant leaf is moved into a sterile greenhouse, the plant leaf is kept stand for 5 hours at a constant temperature and humidity under the conditions that the temperature is 40 ℃ and the humidity is 50%, then ultraviolet sterilization is carried out for 8 hours to obtain a sterilization membrane, the obtained sterilization membrane is dried for 6 hours under the condition that the temperature is 50 ℃, and then the dried sterilization membrane is packaged in vacuum to obtain the artificial skin special for burn. The chitosan is chitosan with deacetylation degree of 95%. The preparation process of the carbomer dispersion comprises the following steps: according to the mass ratio of 1: 15 mixing carbomer with water, and performing ultrasonic dispersion to obtain carbomer dispersion. The biomass surfactant is prepared from lecithin and tea saponin in a mass ratio of 1: 3 is prepared by compounding. The auxiliary agent comprises the following raw materials in parts by weight: 20 parts of oleic acid, 10 parts of linoleic acid and 8 parts of azone. The plant leaves are oleander leaves.

Example 8

Pouring sepiolite into a grinder, grinding, sieving by a 500-mesh sieve to obtain sepiolite powder, and mixing the sepiolite powder with 10% hydrochloric acid according to a mass ratio of 1: 10, mixing and soaking for 6 hours, filtering to obtain a filter cake, drying the obtained filter cake to constant weight at the temperature of 110 ℃ to obtain dry sepiolite powder, transferring the obtained dry sepiolite powder into a tubular furnace, roasting for 5 hours at the temperature of 280 ℃, cooling to room temperature along with the furnace, and discharging to obtain pretreated sepiolite; according to the mass ratio of 1: 15, mixing chitosan and water, pouring the mixture into a No. 1 beaker, stirring and mixing the mixture for 15min by using a glass rod, standing and swelling the mixture for 5h at room temperature, adding chitosan enzyme accounting for 8 percent of the mass of the chitosan into the No. 1 beaker after the swelling is finished, moving the No. 1 beaker into a digital display speed measurement constant-temperature magnetic stirrer, stirring and performing enzymolysis for 5h at a constant temperature of 40 ℃ and a rotating speed of 500r/min, performing heat preservation and enzyme deactivation for 20min at a temperature of 95 ℃, cooling, and discharging the mixture to obtain chitosan enzymatic hydrolysate; according to the weight portion, 10 portions of polyethylene glycol, 10 portions of bacterial cellulose, 0.8 portion of glycerol and 200 portions of dilute acetic acid solution with the mass fraction of 5 percent are taken in sequence, and the polyethylene glycol, the bacterial cellulose and the glycerol are poured into a mortarGrinding and mixing for 20min, pouring the ground material into a No. 2 beaker containing dilute acetic acid solution, stirring and mixing for 15min by using a glass rod, standing and swelling for 6h at room temperature, moving the beaker into a digital display speed measurement constant-temperature magnetic stirrer, and heating, stirring and dispersing for 60min at the temperature of 95 ℃ and the rotating speed of 500r/min to obtain mixed dispersion liquid; according to the weight parts, sequentially taking 30 parts of chitosan enzymolysis liquid, 30 parts of mixed dispersion liquid, 10 parts of onion juice, 15 parts of carbomer dispersion liquid, 0.5 part of nano silver powder, 8 parts of sesame oil, 5 parts of vegetable essential oil, 0.5 part of biomass surfactant and 8 parts of pretreated sepiolite, adding the mixture into a high-speed shearing emulsifying machine, shearing the mixture at a high speed for 20min under the condition that the rotating speed is 12000r/min, and discharging to obtain film-forming emulsion; cutting the lower epidermis of the plant leaf with a blade, marking the two surfaces of the lower epidermis of the plant leaf as an A surface and a B surface respectively, spraying a dopamine solution with the mass concentration of 2g/L on the B surface, and controlling the spraying amount of the dopamine solution to be 3g/cm²After the dopamine solution is sprayed, standing the lower epidermis of the plant leaf at room temperature for 20min, then coating a film-forming emulsion on the surface B, controlling the coating thickness of the film-forming emulsion to be 1.2mm, after the film-forming emulsion is coated, drying the lower epidermis of the plant leaf at a low temperature of 40 ℃ until the water content is 40%, after the drying is finished, spraying a glutaraldehyde ethanol solution with the mass fraction of 10% on the surface B, and controlling the spraying amount of the glutaraldehyde ethanol solution to be 0.3mL/cm²After the glutaraldehyde ethanol solution is sprayed, the lower epidermis of the plant leaf is moved into a sterile greenhouse, the plant leaf is kept stand for 5 hours at a constant temperature and humidity under the conditions that the temperature is 40 ℃ and the humidity is 50%, then ultraviolet sterilization is carried out for 8 hours to obtain a sterilization membrane, the obtained sterilization membrane is dried for 6 hours under the condition that the temperature is 50 ℃, and then the dried sterilization membrane is packaged in vacuum to obtain the artificial skin special for burn. The chitosan is chitosan with deacetylation degree of 95%. The preparation process of the carbomer dispersion comprises the following steps: according to the mass ratio of 1: 15 mixing carbomer with water, and performing ultrasonic dispersion to obtain carbomer dispersion. The plant essential oil is lavender essential oil. The biomass surfactant is prepared from lecithin and tea saponin in a mass ratio of 1: 3 is prepared by compounding. The plant leaves are oleander leaves.

Example 9

Pouring sepiolite into a grinder, grinding, sieving by a 500-mesh sieve to obtain sepiolite powder, and mixing the sepiolite powder with 10% hydrochloric acid according to a mass ratio of 1: 10, mixing and soaking for 6 hours, filtering to obtain a filter cake, drying the obtained filter cake to constant weight at the temperature of 110 ℃ to obtain dry sepiolite powder, transferring the obtained dry sepiolite powder into a tubular furnace, roasting for 5 hours at the temperature of 280 ℃, cooling to room temperature along with the furnace, and discharging to obtain pretreated sepiolite; according to the mass ratio of 1: 15, mixing chitosan and water, pouring the mixture into a No. 1 beaker, stirring and mixing the mixture for 15min by using a glass rod, standing and swelling the mixture for 5h at room temperature, adding chitosan enzyme accounting for 8 percent of the mass of the chitosan into the No. 1 beaker after the swelling is finished, moving the No. 1 beaker into a digital display speed measurement constant-temperature magnetic stirrer, stirring and performing enzymolysis for 5h at a constant temperature of 40 ℃ and a rotating speed of 500r/min, performing heat preservation and enzyme deactivation for 20min at a temperature of 95 ℃, cooling, and discharging the mixture to obtain chitosan enzymatic hydrolysate; according to the weight parts, sequentially taking 10 parts of polyethylene glycol, 10 parts of bacterial cellulose, 0.8 part of glycerol and 200 parts of a dilute acetic acid solution with the mass fraction of 5%, pouring the polyethylene glycol, the bacterial cellulose and the glycerol into a mortar, grinding and mixing for 20min, pouring the ground material into a No. 2 beaker containing the dilute acetic acid solution, stirring and mixing for 15min by using a glass rod, standing and swelling for 6h at room temperature, moving the beaker into a digital display speed measurement constant-temperature magnetic stirrer, and heating, stirring and dispersing for 60min at the temperature of 95 ℃ and the rotating speed of 500r/min to obtain a mixed dispersion liquid; according to the weight parts, sequentially taking 30 parts of chitosan enzymolysis liquid, 30 parts of mixed dispersion liquid, 10 parts of onion juice, 15 parts of carbomer dispersion liquid, 0.5 part of nano silver powder, 8 parts of sesame oil, 5 parts of vegetable essential oil, 0.5 part of biomass surfactant, 8 parts of pretreated sepiolite and 5 parts of auxiliary agent, adding the mixture into a high-speed shearing emulsifying machine, shearing at a high speed for 20min under the condition that the rotating speed is 12000r/min, and discharging to obtain film-forming emulsion; cutting the non-woven fabric by a blade, respectively marking two surfaces of the non-woven fabric as an A surface and a B surface, spraying a dopamine solution with the mass concentration of 2g/L on the B surface, and controlling the spraying amount of the dopamine solution to be 3g/cm²After the dopamine solution is sprayed, standing the non-woven fabric for 20min at room temperature, coating the film-forming emulsion on the surface B, and controlling the coating thickness of the film-forming emulsionThe degree is 1.2mm, after the film-forming emulsion is coated, the non-woven fabric is dried at low temperature under the condition of 40 ℃ until the water content is 40%, after the drying is finished, a glutaraldehyde ethanol solution with the mass fraction of 10% is sprayed on the surface B, and the spraying amount of the glutaraldehyde ethanol solution is controlled to be 0.3mL/cm²After the glutaraldehyde ethanol solution is sprayed, the non-woven fabric is moved into a sterile greenhouse, is kept stand for 5 hours at a constant temperature and humidity under the conditions that the temperature is 40 ℃ and the humidity is 50%, is subjected to ultraviolet sterilization for 8 hours to obtain a sterilization membrane, is dried for 6 hours at a temperature of 50 ℃, and is subjected to vacuum packaging to obtain the artificial skin special for burns. The chitosan is chitosan with deacetylation degree of 95%. The preparation process of the carbomer dispersion comprises the following steps: according to the mass ratio of 1: 15 mixing carbomer with water, and performing ultrasonic dispersion to obtain carbomer dispersion. The plant essential oil is lavender essential oil. The biomass surfactant is prepared from lecithin and tea saponin in a mass ratio of 1: 3 is prepared by compounding. The auxiliary agent comprises the following raw materials in parts by weight: 20 parts of oleic acid, 10 parts of linoleic acid and 8 parts of azone. The plant leaves are oleander leaves.

Comparative example: artificial skin produced by Shantou certain bioengineering Co.

The artificial skin obtained in examples 1 to 9 and the comparative example products were subjected to a performance test by the following specific test method:

the burn wound is known on experimental nude mice, the artificial skin is used for treatment respectively, the healing time is observed, and the sterilization rate is detected. Specific detection results are shown in table 1:

TABLE 1

As can be seen from the test results in Table 1, the artificial skin for burns obtained by the present invention has excellent healing effect and bactericidal effect.

Claims

1. A preparation method of artificial skin special for burns is characterized by comprising the following specific preparation steps:

2. The method for preparing artificial skin for burn according to claim 1, wherein the method comprises the following steps: the chitosan in the step (1) is chitosan with deacetylation degree of 85-95%.

3. The method for preparing artificial skin for burn according to claim 1, wherein the method comprises the following steps: the preparation process of the carbomer dispersion in the step (3) comprises the following steps: according to the mass ratio of 1: 10-1: 15 mixing carbomer with water, and performing ultrasonic dispersion to obtain carbomer dispersion.

4. The method for preparing artificial skin for burn according to claim 1, wherein the method comprises the following steps: the plant essential oil in the step (3) is any one of lavender essential oil, rose essential oil, camellia essential oil or jasmine essential oil.

5. The method for preparing artificial skin for burn according to claim 1, wherein the method comprises the following steps: the biomass surfactant in the step (3) is prepared from lecithin and tea saponin in a mass ratio of 1: 1-1: 3 is prepared by compounding.

6. The method for preparing artificial skin for burn according to claim 1, wherein the method comprises the following steps: the preparation process of the pretreated sepiolite in the step (3) comprises the following steps: the sepiolite is crushed, sieved, soaked by hydrochloric acid, filtered, dried and roasted to obtain the pretreated sepiolite.

7. The method for preparing artificial skin for burn according to claim 1, wherein the method comprises the following steps: the auxiliary agent in the step (3) is prepared from the following raw materials in parts by weight: 10-20 parts of oleic acid, 8-10 parts of linoleic acid and 4-8 parts of azone.

8. The method for preparing artificial skin for burn according to claim 1, wherein the method comprises the following steps: the plant leaf in the step (4) is any one of oleander leaf, aloe leaf, gladiolus leaf or sansevieria leaf.