CN107610724B - Quantitative detecting method based on the full optical disk image forming system of blu-ray drives - Google Patents
Quantitative detecting method based on the full optical disk image forming system of blu-ray drives Download PDFInfo
- Publication number
- CN107610724B CN107610724B CN201710656932.5A CN201710656932A CN107610724B CN 107610724 B CN107610724 B CN 107610724B CN 201710656932 A CN201710656932 A CN 201710656932A CN 107610724 B CN107610724 B CN 107610724B
- Authority
- CN
- China
- Prior art keywords
- data
- blu
- optical disk
- image
- standard
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
A kind of quantitative detecting method based on the full optical disk image forming system of blu-ray drives, the quantitative detecting method are to prepare biological detection arrays on standard Blu-ray Disc；CD is read using standard blu-ray drives, dimension transformation, feature alignment, the mean value scaling, coordinate system transformation of the full optical disk image forming system by collected light intensity signal Jing Guo standard blu-ray drives form full disc image file；Image file analysis to generation, just obtains the quantitative detection result of target biological molecules.This method have many advantages, such as multi-pass amount, high sensitivity, can field quick detection, be suitable for the key areas such as food safety, water quality detection and medical diagnosis.
Description
Technical field
The present invention relates to a kind of analysis of imaging of biomolecules and quantitative detecting method, specifically it is a kind of based on LabVIEW,
The quantitative detecting method of the full optical disk image forming system of the standard blu-ray drives of MATLAB and C++ hybrid programming.
Background technique
Year ends 1970, optical disc come out, and since optical disc surface is very smooth and has good optical property, pass through
The optical disc surface of processing can be with specific binding protein, DNA probe molecule, and then can prepare detection array in optical disc surface
Structure, when above CD-ROM system laser beam flying CD to CD detection array, laser beam path is interfered, with photoelectricity two
Pole pipe measuring beam intensity will be reduced accordingly.Wherein, the optical signalling amplitude variation that photodetector receives in optical head
It can be used for quantitative analysis.
Early in 2000, the Kido in the U.S. et al. (Kido H, Maquieira A, Hammock B D. Disc-
based immunoassay microarrays[J]. Analytica Chimica Acta, 2000, 411(1-2): 1-
11) it is prepared for the protein microarray of the fluorescent marker based on immune response in common CD optical disc surface, uses Fluorescence Scanner
It is tested and analyzed, and is proposed and any hardware modification is not carried out to CD-ROM drive and can not realize " biochemistry CD " signal
Identification and specificity read " software solution " imagination.2003, La Clair et al. (La Clair J J,
Burkart M D. Molecular screening on a compact disc[J]. Organic & Biomolecular
Chemistry, 2003,1 (18): 3244-9) based on standard CD-ROM drive, it is compared and is divided by using the software that design is write
The initial data wrong data corresponding with the CD after molecule association reaction for analysing CD, goes out ligand-for quantitative screening
Association reaction between protein.This method screens the ligand and biology point of CD optical disc surface expression by error mensuration program
Son, it is accurate without DVD and BD CD-ROM drive due to using CD drive, and because PC is incompatible with there is pole solvent, to the surface CD-R
It is activated by phosphorylation attaching ligand molecule to be extremely difficult in acetonitrile.
The Potyrailo in the U.S. et al. (Radislav A. Potyrailo, William G. Morris, Andrew
M. Leach, et al. Analog Signal Acquisition from Computer Optical Disk Drives
for Quantitative Chemical Sensing[J]. Analytical Chemistry, 2006, 78(16):
5893-9) from CD-ROM driver's printed circuit board extract optical head photodetector analog signal, and Usage data collection card acquisition and
LabVIEW writes program and CD-ROM drive is controlled and is imaged, and realizes the quantitative detection of the metal ion of CD optical disc surface.But this
Kind system only realizes array image-forming, is not able to achieve full optical disk image forming, and flux is not also high, does not utilize individual light adequately
Disk will cause the redundancy of experiment number.In patent (Radislav Alexandrovich Potyrailo. Sensor
systems and methods for quantifications of physical parameters, chemical and
Biochemical volatile and nonvoiatile compounds in fluids: the U.S., 7,170,609 B2 of US
[P] .2007-1-30) in, Potyrailo et al. extracts light intensity signal from photoelectric detector, quantifies transmission by analog-to-digital conversion
The intensity of light, to obtain the concentration of compound on CD.But, there is no to full optical disk image forming in the patent.
Hispanic Maquieira research group (Morais S, Carrascosa J, Mira D, et al.
Microimmunoanalysis on standard compact discs to determine low abundant
Compounds [J] Analytical Chemistry, 2007,79 (20): 7628-7635.) it is then using semi-transparent semi-reflecting
Special CD, an Optical Sensing Trigger and plane photodiode receiver are increased above CD-ROM drive, extracts light in real time
The transmitted light intensity signal of conversion zone is analyzed on disk.This method is in addition to that will be arranged special CD, it is also necessary in CD-ROM drive
Other hardware devices are installed, it is more bothersome, and need to rely on the business softwares such as photoshop in post-processing data.
Various forms of transformations of CD-ROM drive become the platform technology of novel molecular recognition and detection, still, due to
The particularity of transformation, these transformations will affect CD-ROM drive and read the ability of CD or even destroy CD-ROM drive, and it is special that some also needs to make
CD, these all largely reduce the advantage based on CD/CD-ROM drive molecular recognition and detection technique.The present invention
CD is neither transformed in the method that people proposes, and realizes full optical disk image forming, while solving to detect in standard CD-ROM drive detection method and lead to
The limited problem of amount.
Summary of the invention
It is an object of the invention to based on blu-ray drives read-write theory and CD storage technique, by extracting, acquiring
The light intensity signal of photoelectric detector inside standard blu-ray drives optical heads, using being researched and developed under a windows environment based on standard
The full optical disk image forming system of blu-ray drives realizes biomolecule high-precision, high-throughput rapid detection method, and provides a kind of base
In the quantitative detecting method of the full optical disk image forming system of blu-ray drives.
To achieve the goals above, the present invention provides a kind of quantitative detection side based on the full optical disk image forming system of blu-ray drives
Method, the method follow these steps to carry out:
Biological detection arrays are formed using microflow control technique in Blu-ray Disc polycarbonate surface；
CD is scanned using blu-ray drives, during CD scanning, using data collecting card from optical head Photoelectric Detection
The full optical disk image forming system of standard blu-ray drives in the collected data synchronous transfer to computer of device；
Collected data are subjected to dimension transformation using the full optical disk image forming system of standard blu-ray drives, feature is aligned,
Full disc image file is formed after value scaling, coordinate system transformation；
Using the image analysis module of the full optical disk image forming system of standard blu-ray drives to the pixel of image file biologic array
Value carries out linear fit, obtains target biological molecules concentration；
Realize imaging analysis and quantitative detection.
In the above-mentioned technical solutions, the further additional technical feature of the present invention is:
The quantitative detecting method is to put the CD after biological respinse well, is not doing biological respinse with metallochrome marker pen
Data of optical disk area, using CD radius as directrix, mark triggers band as acquisition data characteristic value.
The quantitative detecting method be will will acquisition storage 16 file of I in one-dimensional data according to sampling length gradually
Reduction sampling process simultaneously sequentially inputs elliptic filter, and using low-pass filtering, filtered data are stored by interception position, storage
Data are 2-D data in file afterwards, realize acquisition data dimension conversion, to the 2-D data after dimension transformation by pair
Single acquisition data carry out Envelope Analysis, meet characteristic quantity position mark to width and amplitude, and intercept the data of corresponding length
Primary storage, so that 2-D data completes feature alignment, by the data procession format length after alignment, i.e., by setting row
Several and columns expands or compresses in a manner of being rounded nearby, so that data realize mean value scaling.
The quantitative detecting method is the image generation module in the full optical disk image forming system of standard blu-ray drives by mean value
2-D data after scaling, by the operation of following formula:
Wherein: m, n are original coordinates；X, y are to meet actual pixels coordinate value；R is imaging region start radius；Δ r is
The space length of adjacent rows；Δ θ is the angle that adjacent two point data corresponds to space in data line, realizes each data
Revert to the corresponding position on full disc image.
The quantitative detecting method is the Analysis of test results module in the full optical disk image forming system of standard blu-ray drives, will
Tif format image files are converted into bianry image, then utilize the data interaction function of Analysis of test results module, when click pair
When answering the arbitrary region in bianry image, pixel mean value size in the region enveloping space can be provided in display box, is being detected
Filling mark is separately added into Standard-concentration and Standard-pixel value in interpretation of result module
The concentration and respective image pixel value of quasi- sample are inserted sample to be tested pixel value in Sample-pixel value, are set up
Testing result can be provided at rear click ' the Fitting Calculation ' button.
This method with standard CD-ROM drive and carries standard blu-ray drives based on blu-ray drives reading principle and memory technology
Full optical disk image forming system computer be platform, overcome transformation CD-ROM drive time-consuming, it is expensive the problems such as, with existing biology
Molecular method quantification detection method is compared, and full optical disk image forming may be implemented, and detection flux is higher.
What this method utilized is standard blu-ray drives, and compared with CD, DVD drive, the focal beam spot diameter of laser is smaller,
Detection resolution is higher, has higher detection sensitivity.
The micro-concentrations of this method heavy metal etc. in harmful components imaging analysis food, pesticide residue, water,
It can be widely applied to the fields such as clinical examination, diagnosis, food industry and individual medical treatment, before there is boundless market
Scape.
Detailed description of the invention
Fig. 1 is the full optical disk image forming system schematic of this method standard blu-ray drives.
Fig. 2 is the full optical disk image forming figure of this method biology dot matrix.
Fig. 3 is the matched curve figure of this method standard sample and sample to be tested.
Specific embodiment
A specific embodiment of the invention is made further instructions below.
Implement a kind of quantitative detecting method based on the full optical disk image forming system of blu-ray drives provided by the present invention, including this
The CD that biologic array will be present and carry out triggering band of inventive method use puts CD-ROM drive acquisition data, the data after acquisition into
By dimension transformation, feature alignment, mean value scaling, coordinate system transformation, full disc image file is formed, to the image file of generation
The concentration of target biological molecules is obtained after data interaction, the Fitting Calculation, specific detection method follows these steps to carry out:
(1) biological respinse of CD: biological detection battle array is formed using microflow control technique in Blu-ray Disc polycarbonate surface
Column.
(2) mark triggers band: the CD after biological respinse is put well, is not doing biological respinse with metallochrome marker pen
Data of optical disk area, using the radius of CD as directrix, characteristic value of the mark triggers band as acquisition data.
(3) data are acquired: the CD for forming biologic array after biological respinse being put into external blu-ray drives, is utilized
Track wrong (TE) and focal error (FE) in PlexUTILITIES software, make CD start to rotate with 6 times of revolving speeds, data
In collection process, full optical disk image forming system parameter configuration in data acquisition of standard blu-ray drives: coupled modes are used
AC coupled, trigger source are ATR triggerings, and triggering direction is that lower edges trigger, and channel is set as ± 1000mv, sampling frequency
Rate is 2MHz, and clock source is internal clocking.Data collecting card starts to acquire the light intensity signal of photoelectric detector, while will collect
Data pass to the full optical disk image forming systems of standard blu-ray drives, collected data are stored as I16 file.
(4) it acquires data processing: the one-dimensional data in the I16 storage file after acquisition is gradually restored according to sampling length
Sampling process simultaneously sequentially inputs elliptic filter, and low-pass filtering, ± 1000 mv of measurement range selection, filtered number are used when filtering
It is stored according to by interception position, data are two dimension in data end per treatment insertion newline storage, the file after storage
Data realize the dimension transformation of acquisition data.2-D data after dimension transformation is carried out by the data to single acquisition
Envelope Analysis, meets width and amplitude the position mark of characteristic quantity, and the data for intercepting corresponding length once store, so that two
Dimension data completes feature alignment, and the data after alignment are stored as txt format.When 2-D data feature is aligned, setting triggering band
It is upper and lower be limited to 10000 ~ 25000, individual pen data volume up and down be limited to 100 ~ 250 and trigger band threshold value.After alignment
Data procession format length is expanded or is compressed in a manner of being rounded nearby by setting line number and columns, so that data
Realize mean value scaling.
(5) full optical disk image forming and quantitative detection: in the image generation module of the full optical disk image forming system of standard blu-ray drives
2-D data after mean value is scaled realizes that each data convert arrives by the transformation from rectangular coordinate system to polar coordinate system
Corresponding position on disc image, i.e., by a rectangular image by forming a doughnut after rectangular coordinates transformation to polar coordinates
Picture, the image of generation is closer to actual CD.When image generates, need to be arranged the CD center of circle to the most short distance for triggering band
Ranks number from the 2-D data after R0, triggering band length Δ R, alignment columns and mean value scaling, to obtain original adopt
The two pseudo- chromaticity diagrams (jpg and tif) generated after collection data processing optimization.In the full optical disk image forming system of standard blu-ray drives
Tif format image files are converted into bianry image by Analysis of test results module, using data interaction function when click corresponding two
When being worth the arbitrary region in image, pixel mean value in the region enveloping space can be provided in Sample_intensity display box
Size.The concentration of filling standard sample is separately added into Standard-intensity and Standard-concentration
With respective image gray value, sample to be tested gray value is inserted in Sample-intensity, and ' fitting meter is clicked after being provided with
Calculation ' button can provide testing result.
Below by taking determinand aflatoxins (AFB1) as an example, a kind of imaging of biomolecules that present invention be described in more detail
Analysis and quantitative detecting method, specific embodiment follow these steps to carry out:
Step 1: the biological respinse of CD
Biological detection arrays are formed using microflow control technique in Blu-ray Disc polycarbonate surface.
Step 2: mark triggers band
CD after biological respinse is put well, with metallochrome marker pen in the data of optical disk area for not doing biological respinse, with light
The radius of disk is directrix, characteristic value of the mark triggers band as acquisition data.
Step 3: acquisition data
The CD for forming biologic array after biological respinse is put into external blu-ray drives, utilizes PlexUTILITIES software
In track wrong (TE) and focal error (FE), make CD start to rotate with 6 times of revolving speeds, in data acquisition, standard blue
Full optical disk image forming system parameter configuration in data acquisition of light CD-ROM drive: coupled modes use AC coupled, and trigger source is
ATR triggering, triggering direction is that lower edges trigger, and channel is set as ± 1000mv, sample frequency 2MHz, and clock source is
Internal clocking.Data collecting card starts to acquire the light intensity signal of photoelectric detector, while collected data are passed to standard
The full optical disk image forming system of blu-ray drives, collected data are stored as I16 file.
Step 4: acquisition data processing
One-dimensional data in I16 storage file after acquisition is gradually restored according to sampling length to sampling process and successively defeated
Enter elliptic filter, when filtering, uses low-pass filtering, ± 1000 mv of measurement range selection, filtered data by interception position storage,
Data are 2-D data in data end per treatment insertion newline storage, the file after storage, realize acquisition number
According to dimension transformation.Envelope Analysis is carried out by data to single acquisition to the 2-D data after dimension transformation, to width and
Amplitude meets the position mark of characteristic quantity, and the data for intercepting corresponding length once store, so that 2-D data completes feature pair
Together, the data after alignment are stored as txt format.When 2-D data feature is aligned, setting triggering band it is upper and lower be limited to 10000 ~
25000, individual pen data volume is limited to 100 ~ 250 and triggers the threshold value of band be -0.2 up and down.Data after alignment are gone
Column format length is 1471 by setting line number and columns, is expanded in a manner of being nearly rounded or compressed, so that data are real
Existing mean value scaling.
Step 5: full optical disk image forming and quantitative detection
2-D data after the image generation module of the full optical disk image forming system of standard blu-ray drives scales mean value, warp
The transformation from rectangular coordinate system to polar coordinate system is crossed, the corresponding position on each data convert to disc image is realized, i.e., will
One rectangular image is by forming an annulus image after rectangular coordinates transformation to polar coordinates, the image of generation is closer to actual light
Disk.When image generates, the shortest distance R0 for needing to be arranged the CD center of circle to triggering band is 2.4, triggering band length Δ R is
3.6, the ranks number of the 2-D data after alignment columns is 98 and mean value scaling is 1471, to obtain at acquired original data
The pseudo- chromaticity diagram (jpg and tif) of two generated after reason optimization.In the testing result of the full optical disk image forming system of standard blu-ray drives
Tif format image files are converted into bianry image by analysis module, using data interaction function when in the corresponding bianry image of click
Arbitrary region when, pixel mean value size in the region enveloping space can be provided in Sample_intensity display box.?
The concentration of filling standard sample and corresponding is separately added into Standard-intensity and Standard-concentration
Gray value of image inserts sample to be tested gray value in Sample-intensity, and ' the Fitting Calculation ' is clicked after being provided with and is pressed
Button can provide testing result.
Claims (4)
1. a kind of quantitative detecting method based on the full optical disk image forming system of blu-ray drives, the method follow these steps to carry out:
Biological detection arrays are formed using microflow control technique in Blu-ray Disc polycarbonate surface；
It is adopted using data collecting card from optical head photoelectric detector during CD scanning using blu-ray drives scanning CD
The full optical disk image forming system of standard blu-ray drives in the data synchronous transfer to computer collected；
Collected data are subjected to dimension transformation, feature alignment, mean value contracting using the full optical disk image forming system of standard blu-ray drives
It puts, form full disc image file after coordinate system transformation；
Using the full optical disk image forming system of standard blu-ray drives image analysis module to the pixel value of image file biologic array into
Row linear fit obtains target biological molecules concentration；
Realize imaging analysis and quantitative detection；
The imaging analysis and quantitative detection are incited somebody to action in the image generation module of the full optical disk image forming system of standard blu-ray drives
2-D data after mean value scaling realizes each data convert to light by the transformation from rectangular coordinate system to polar coordinate system
Corresponding position on disk image, i.e., by a rectangular image by forming an annulus image after rectangular coordinates transformation to polar coordinates,
The image of generation is closer to actual CD；When image generates, need to be arranged the CD center of circle to the shortest distance for triggering band
The ranks number of 2-D data after R0, triggering band length Δ R, alignment columns and mean value scaling, to obtain acquired original
The pseudo- chromaticity diagram of two generated after data processing optimization；In the Analysis of test results of the full optical disk image forming system of standard blu-ray drives
Tif format image files are converted into bianry image by module, using data interaction function when appointing in the corresponding bianry image of click
When meaning region, pixel mean value size in the region enveloping space can be provided in Sample_intensity display box；?
The concentration of filling standard sample and corresponding is separately added into Standard-intensity and Standard-concentration
Gray value of image inserts sample to be tested gray value in Sample-intensity, and ' the Fitting Calculation ' is clicked after being provided with and is pressed
Button can provide testing result.
2. quantitative detecting method as described in claim 1, the quantitative detecting method is to put the CD after biological respinse well,
With metallochrome marker pen in the data of optical disk area for not doing biological respinse, using CD radius as directrix, mark triggers band is used as and adopts
Collect the characteristic value of data.
3. quantitative detecting method as described in claim 1, the quantitative detecting method is in 16 file of I for will acquire storage
One-dimensional data gradually restore sampling process according to sampling length and sequentially input elliptic filter, using low-pass filtering, filtering
Data afterwards are stored by interception position, and data are 2-D data in the file after storage, realize acquisition data dimension conversion, right
2-D data after dimension transformation meets characteristic quantity position to width and amplitude by carrying out Envelope Analysis to single acquisition data
Label, and the data for intercepting corresponding length once store, so that 2-D data completes feature alignment, the data after alignment are carried out
Ranks format length is expanded or is compressed in a manner of being rounded nearby by setting line number and columns, so that data realize mean value
Scaling.
4. quantitative detecting method as claimed in claim 3, the quantitative detecting method is the full CD in standard blu-ray drives
The image generation module of imaging system mean value is scaled after 2-D data, by the operation of following formula:
Wherein: m, n are original coordinates；X, y are to meet actual pixels coordinate value；R is imaging region start radius；Δ r is adjacent
The space length of two rows；Δ θ is the angle that adjacent two point data corresponds to space in data line, realizes each data convert
Corresponding position onto full disc image.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710656932.5A CN107610724B (en) | 2017-08-03 | 2017-08-03 | Quantitative detecting method based on the full optical disk image forming system of blu-ray drives |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710656932.5A CN107610724B (en) | 2017-08-03 | 2017-08-03 | Quantitative detecting method based on the full optical disk image forming system of blu-ray drives |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107610724A CN107610724A (en) | 2018-01-19 |
| CN107610724B true CN107610724B (en) | 2019-09-27 |
Family
ID=61064717
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710656932.5A Active CN107610724B (en) | 2017-08-03 | 2017-08-03 | Quantitative detecting method based on the full optical disk image forming system of blu-ray drives |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107610724B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7170609B2 (en) * | 2003-11-24 | 2007-01-30 | General Electric Company | Sensor systems and methods for quantification of physical parameters, chemical and biochemical volatile and nonvolatile compounds in fluids |
| CN1906489A (en) * | 2003-11-24 | 2007-01-31 | 通用电气公司 | Optical storage medium having an analyte containing polymer film, use thereof |
| CN104991416A (en) * | 2015-07-23 | 2015-10-21 | 太原理工大学 | Optical disc based dimensional periodic micro-nano structure hot padding method |
| CN106442418A (en) * | 2016-09-06 | 2017-02-22 | 大连理工大学 | Low-cost spectroscopic imaging system based on surface plasma resonance |
-
2017
- 2017-08-03 CN CN201710656932.5A patent/CN107610724B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7170609B2 (en) * | 2003-11-24 | 2007-01-30 | General Electric Company | Sensor systems and methods for quantification of physical parameters, chemical and biochemical volatile and nonvolatile compounds in fluids |
| CN1906489A (en) * | 2003-11-24 | 2007-01-31 | 通用电气公司 | Optical storage medium having an analyte containing polymer film, use thereof |
| CN104991416A (en) * | 2015-07-23 | 2015-10-21 | 太原理工大学 | Optical disc based dimensional periodic micro-nano structure hot padding method |
| CN106442418A (en) * | 2016-09-06 | 2017-02-22 | 大连理工大学 | Low-cost spectroscopic imaging system based on surface plasma resonance |
Non-Patent Citations (4)
| Title |
|---|
| High density MicroArrays on Blu-ray discs for massive screening;Tania Arnandis-Chover,et al;《Biosensors and Bioelectronics》;20140115;第51卷;第109-114页 * |
| New disc-based technologies for diagnostic and research;Raj Barathur,et al;《Psychiatric Genetics》;20020719;第12卷(第4期);第193-206页 * |
| 基于标准光盘/光驱的数字化分子检测技术研究进展;李晓春,等;《科学通报》;20130330;第58卷(第9期);第777-782页 * |
| 基于蓝光技术的数字化分子诊断与检测技术;徐鹏涛,等;《分析测试学报》;20161225;第35卷(第12期);第1601-1605页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107610724A (en) | 2018-01-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20220136030A1 (en) | Analysis and screening of cell secretion profiles | |
| EP2313787B1 (en) | Apparatus, method and article to perform assays using assay strips | |
| AU2008261935B2 (en) | Methods and processes for calling bases in sequence by incorporation methods | |
| CN103109345B (en) | The Dynamic data exchange of product ion spectrum obtains and reference spectra storehouse coupling | |
| US20030215791A1 (en) | Method of and system for multiplexed analysis by spectral imaging | |
| CN108645824B (en) | Sensor array chip and preparation method and application thereof | |
| EP1425696A2 (en) | Methods for differential cell counts including related apparatus and software for performing same | |
| US10885673B2 (en) | Method for identifying a unit using a camera | |
| De Lannoy et al. | Evaluation of FRET X for single-molecule protein fingerprinting | |
| CN107610724B (en) | Quantitative detecting method based on the full optical disk image forming system of blu-ray drives | |
| Hussain | Smartphone-based detection devices: Emerging trends in analytical techniques | |
| US11099179B2 (en) | Device and method for detecting substances present in biological or chemical samples | |
| US7732217B2 (en) | Apparatus and method for reading fluorescence from bead arrays | |
| DE10127221A1 (en) | Carrier, for analysis of chemical or biological sensor molecules, has geometrical surface layout for samples, according to scanning method | |
| CN207816824U (en) | Antigen detection device based on upper conversion nano particle marker and linear light source excitation | |
| CN1281954C (en) | Apparatus for detecting special component in liquid | |
| Hemmateenejad et al. | Data acquisition and data analysis in colorimetric sensor arrays | |
| JP6228206B2 (en) | Protein-specific light detection | |
| EP3951360B1 (en) | Analysis device and analysis method | |
| CN112611701A (en) | Circulating tumor cell detection device based on dynamic coherent optical imaging technology | |
| CN117551743A (en) | Digital biochemical analysis method based on holographic imaging and matched detection equipment thereof | |
| CN1566953A (en) | Protein fingerprint method for biological sample analysis | |
| CN1595162A (en) | Use of protein biological chips in detecting biological marks in blood of SARS patients | |
| CN1737567A (en) | Application of protein finger mark in blood by detecting myocardial infarction using protein finger mark |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |