CN107510863A - A kind of compound rush CO2 laser weld pipe and preparation method thereof - Google Patents
A kind of compound rush CO2 laser weld pipe and preparation method thereof Download PDFInfo
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- CN107510863A CN107510863A CN201710740413.7A CN201710740413A CN107510863A CN 107510863 A CN107510863 A CN 107510863A CN 201710740413 A CN201710740413 A CN 201710740413A CN 107510863 A CN107510863 A CN 107510863A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/26—Mixtures of macromolecular compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/16—Materials with shape-memory or superelastic properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/32—Materials or treatment for tissue regeneration for nerve reconstruction
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Abstract
The invention discloses a kind of compound rush CO2 laser weld pipe and preparation method thereof.The compound rush CO2 laser weld pipe is made up of following components by weight：50 ~ 100 parts of gelatin；20 ~ 50 parts of chitosan；10 ~ 25 parts of hyaluronic acid；10 ~ 20 parts of fibroin；0.2 ~ 1 part of coupling agent；The present invention uses main material of the composite of gelatin, chitosan, hyaluronic acid, fibroin as rush CO2 laser weld pipe, improves its structural strength, and avoids being swelled, and has been obviously improved the functional restoration effect to sciatic nerve.
Description
Technical field
The present invention relates to medical surgery technical field, is specifically a kind of compound rush CO2 laser weld pipe.
Background technology
Current various wounds such as are oppressed, drawing-off, tear, cut off the part or all of damage that reason causes nervous system,
So as to which nerve causes function to be lost and other neurogenic diseases.For there is the nerve damaged, neural neuron axon is suitable
It can be regenerated in the case of.The development of micro- neurosurgery is particularly by neurosurgery, the reparation and regeneration of neurotrosis are
It is possibly realized.The CO2 laser weld technology studied and clinically applied at present mainly has：Directly suture, nerve-grafting (autotransplantation
Or heteroplastic transplantation) and by promoting CO2 laser weld pipe reparation.Directly it coincide and is only limited to shorter neurectmesis (being less than 5mm), it is no
Can then there is the tension force that coincide, be unfavorable for nerve regneration；Nerve-grafting by donor due to being limited, it is difficult to generally uses；And promote
CO2 laser weld pipe is mainly used in the long CO2 laser weld blocked, and its neural functional restoration is not clinically very satisfied.
In the case of tension-free, direct nerve suture is clinical means best at present.But nerve suture is directly carried out, its is postoperative
The recovery of the organ dysfunction of innervation is nor very well.Therefore the use of the auxiliary nervous recovery of nerve trachea is a kind of fine
Means.
" one kind promotees CO2 laser weld pipe and preparation method thereof and should Application No. CN201610345822.2 patent of invention
With " disclose that " the present invention relates to bioengineering field, and in particular to one kind promotees CO2 laser weld pipe and its preparation method and application.
In order to overcome the problem of neurological functional recovery is bad after current direct neural suture, the invention provides one kind to have shape note
Recall the rush CO2 laser weld pipe of performance.The CO2 laser weld pipe be will gelatin solution inject CO2 laser weld pipe mold in prepare shaping and
Into.And its neural pipe mold can be to pass through CO2 laser weld pipe mold made from 3D printing.This rush CO2 laser weld pipe has shape
The function of memory, after implanting, it can be returned to the original form in the presence of water, it is automatically axially extending along nerve, so as to
Preferably parcel is covered in around injured nerve, and the growth for nerve provides a stable microenvironment.It can also subtract significantly
The surface of a wound of minor operation separation nerve, is advantageous to wound recovery, promotes the functional rehabilitation of nerve, before having good clinical practice
Scape ".Only it is the material using gelatin single composition as rush CO2 laser weld pipe in above-mentioned patent, although can play a role, its
Toughness of material and intensity are poor, it may occur that collapse, are swelled, tube chamber is diminished, hinder nerve regneration.
Therefore the present invention sets about studying a kind of main material of composite as rush CO2 laser weld pipe, improves its structural strength,
And avoid being swelled.
The content of the invention
It is an object of the invention to provide a kind of composite using gelatin, chitosan, hyaluronic acid, fibroin as rush
The compound rush CO2 laser weld pipe of the main material of CO2 laser weld pipe, to solve the problems mentioned in the above background technology.
To achieve the above object, the present invention provides following technical scheme：
A kind of compound rush CO2 laser weld pipe, is made up of following components by weight：
As the further scheme of the present invention：The gelatin is medicinal bone gelatin.
As the further scheme of the present invention：The coupling agent is EDC-HCl.
A kind of compound preparation method for promoting CO2 laser weld pipe, comprises the following steps：
Step 1: the methanol suspension for the gelatin that mass percent is 1~10%, the methanol suspension of chitosan is respectively configured
Liquid, the methanol solution of hyaluronic acid, the methanol solution of the methanol suspension of fibroin and coupling agent；
It is Step 2: the methanol of the methanol suspension of gelatin in step 1, the methanol suspension of chitosan, hyaluronic acid is molten
The methanol solution blending of liquid, the methanol suspension of fibroin and coupling agent, is warming up to 60~90 DEG C, stirring reaction 1~6 hour, takes out
Filter, is washed with absolute methanol repeatedly, until washing not reacted coupling agent, after 50~70 DEG C are dried in vacuo 6~24 hours, is obtained
To composite；
Step 3: the composite obtained in step 2 is dissolved in distilled water, with mass percent 1~10%
The aqueous solution of composite, deaeration, obtain the aqueous solution of the composite after deaeration；
Step 4: by the aqueous solution of the composite obtained in step 3, injection promotees in CO2 laser weld pipe mold, is placed into
Reacted in less than -20~-5 DEG C environment to gel forming；
Step 5, the rush CO2 laser weld pipe being molded in step 4 is placed on freeze dryer and freezed through 30~50 hours to remove it
In water.
As the further scheme of the present invention：Rush CO2 laser weld pipe mold in the step 4 promotees nerve for high printing opacity PC
Agent for repairing tube mould has.
As the further scheme of the present invention：Rush CO2 laser weld pipe mold in the step 4 promotees CO2 laser weld for metal
Pipe mold.
As the further scheme of the present invention：Rush CO2 laser weld pipe mold in the step 4 is made by 3D printing.
As the further scheme of the present invention：Rush CO2 laser weld pipe after being freezed in the step 5 is pressed by mould
Contracting, be compressed to a diameter of original 40~50%, then by mould reaming, reaming to internal diameter increase to former internal diameter 100~
120%.
As the further scheme of the present invention：The mould of the compression and reaming is made by 3D printing.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention is using gelatin, chitosan, hyaluronic acid, fibroin
Composite as the main material for promoting CO2 laser weld pipe, improve its structural strength, and avoid being swelled, be obviously improved to ischium
The functional restoration effect of nerve.
Embodiment
The technical scheme of this patent is described in more detail with reference to embodiment.
Embodiment one
A kind of compound rush CO2 laser weld pipe, is made up of following components by weight：
Preferably, gelatin is medicinal bone gelatin.
Preferably, coupling agent EDC-HCl.
A kind of preparation method for promoting CO2 laser weld pipe, comprises the following steps：
Step 1: the methanol suspension for the gelatin that mass percent is 1~10%, the methanol suspension of chitosan is respectively configured
Liquid, the methanol solution of hyaluronic acid, the methanol solution of the methanol suspension of fibroin and coupling agent；
It is Step 2: the methanol of the methanol suspension of gelatin in step 1, the methanol suspension of chitosan, hyaluronic acid is molten
The methanol solution blending of liquid, the methanol suspension of fibroin and coupling agent, is warming up to 60~90 DEG C, stirring reaction 1~6 hour, takes out
Filter, is washed with absolute methanol repeatedly, until washing not reacted coupling agent, after 50~70 DEG C are dried in vacuo 6~24 hours, is obtained
To composite；
Step 3: the composite obtained in step 2 is dissolved in distilled water, with mass percent 1~10%
The aqueous solution of composite, deaeration, obtain the aqueous solution of the composite after deaeration；
Step 4: by the aqueous solution of the composite obtained in step 3, injection promotees in CO2 laser weld pipe mold, is placed into
Reacted in less than -20~-5 DEG C environment to gel forming；
Step 5, the rush CO2 laser weld pipe being molded in step 4 is placed on freeze dryer and freezed through 30~50 hours to remove it
In water.
Preferably, the rush CO2 laser weld pipe mold in step 4 is to be repaiied by the personalized nerve that promotees indirectly made from 3D printing
Multiple pipe mold or metal promote CO2 laser weld pipe mold indirectly.
Preferably, CO2 laser weld pipe mold is promoted in step 4 before the aqueous solution of injection composite by lyophilized place
Reason.
Preferably, the rush CO2 laser weld pipe after being freezed in step 5 is compressed by mould, is compressed to a diameter of original
40~50%, the 100~120% of former internal diameter are then increased to by mould reaming, reaming to internal diameter.
Embodiment two
A kind of compound rush CO2 laser weld pipe, is made up of following components by weight：
A kind of preparation method for promoting CO2 laser weld pipe, comprises the following steps：
Step 1: the methanol suspension for the gelatin that mass percent is 1~10%, the methanol suspension of chitosan is respectively configured
Liquid, the methanol solution of hyaluronic acid, the methanol solution of the methanol suspension of fibroin and coupling agent；
It is Step 2: the methanol of the methanol suspension of gelatin in step 1, the methanol suspension of chitosan, hyaluronic acid is molten
The methanol solution blending of liquid, the methanol suspension of fibroin and coupling agent, is warming up to 60~90 DEG C, stirring reaction 1~6 hour, takes out
Filter, is washed with absolute methanol repeatedly, until washing not reacted coupling agent, after 50~70 DEG C are dried in vacuo 6~24 hours, is obtained
To composite；
Step 3: the composite obtained in step 2 is dissolved in distilled water, with mass percent 1~10%
The aqueous solution of composite, deaeration, obtain the aqueous solution of the composite after deaeration；
Step 4: by the aqueous solution of the composite obtained in step 3, injection promotees in CO2 laser weld pipe mold, is placed into
Reacted in less than -20~-5 DEG C environment to gel forming；
Step 5, the rush CO2 laser weld pipe being molded in step 4 is placed on freeze dryer and freezed through 30~50 hours to remove it
In water.
Embodiment three
A kind of compound rush CO2 laser weld pipe, is made up of following components by weight：
A kind of preparation method for promoting CO2 laser weld pipe, comprises the following steps：
Step 1: the methanol suspension for the gelatin that mass percent is 1~10%, the methanol suspension of chitosan is respectively configured
Liquid, the methanol solution of hyaluronic acid, the methanol solution of the methanol suspension of fibroin and coupling agent；
It is Step 2: the methanol of the methanol suspension of gelatin in step 1, the methanol suspension of chitosan, hyaluronic acid is molten
The methanol solution blending of liquid, the methanol suspension of fibroin and coupling agent, is warming up to 60~90 DEG C, stirring reaction 1~6 hour, takes out
Filter, is washed with absolute methanol repeatedly, until washing not reacted coupling agent, after 50~70 DEG C are dried in vacuo 6~24 hours, is obtained
To composite；
Step 3: the composite obtained in step 2 is dissolved in distilled water, with mass percent 1~10%
The aqueous solution of composite, deaeration, obtain the aqueous solution of the composite after deaeration；
Step 4: by the aqueous solution of the composite obtained in step 3, injection promotees in CO2 laser weld pipe mold, is placed into
Reacted in less than -20~-5 DEG C environment to gel forming；
Step 5, the rush CO2 laser weld pipe being molded in step 4 is placed on freeze dryer and freezed through 30~50 hours to remove it
In water.
Example IV
A kind of compound rush CO2 laser weld pipe, is made up of following components by weight：
A kind of preparation method for promoting CO2 laser weld pipe, comprises the following steps：
Step 1: the methanol suspension for the gelatin that mass percent is 1~10%, the methanol suspension of chitosan is respectively configured
Liquid, the methanol solution of hyaluronic acid, the methanol solution of the methanol suspension of fibroin and coupling agent；
It is Step 2: the methanol of the methanol suspension of gelatin in step 1, the methanol suspension of chitosan, hyaluronic acid is molten
The methanol solution blending of liquid, the methanol suspension of fibroin and coupling agent, is warming up to 60~90 DEG C, stirring reaction 1~6 hour, takes out
Filter, is washed with absolute methanol repeatedly, until washing not reacted coupling agent, after 50~70 DEG C are dried in vacuo 6~24 hours, is obtained
To composite；
Step 3: the composite obtained in step 2 is dissolved in distilled water, with mass percent 1~10%
The aqueous solution of composite, deaeration, obtain the aqueous solution of the composite after deaeration；
Step 4: by the aqueous solution of the composite obtained in step 3, injection promotees in CO2 laser weld pipe mold, is placed into
Reacted in less than -20~-5 DEG C environment to gel forming；
Step 5, the rush CO2 laser weld pipe being molded in step 4 is placed on freeze dryer and freezed through 30~50 hours to remove it
In water.
Embodiment five
A kind of compound rush CO2 laser weld pipe, is made up of following components by weight：
A kind of preparation method for promoting CO2 laser weld pipe, comprises the following steps：
Step 1: the methanol suspension for the gelatin that mass percent is 1~10%, the methanol suspension of chitosan is respectively configured
Liquid, the methanol solution of hyaluronic acid, the methanol solution of the methanol suspension of fibroin and coupling agent；
It is Step 2: the methanol of the methanol suspension of gelatin in step 1, the methanol suspension of chitosan, hyaluronic acid is molten
The methanol solution blending of liquid, the methanol suspension of fibroin and coupling agent, is warming up to 60~90 DEG C, stirring reaction 1~6 hour, takes out
Filter, is washed with absolute methanol repeatedly, until washing not reacted coupling agent, after 50~70 DEG C are dried in vacuo 6~24 hours, is obtained
To composite；
Step 3: the composite obtained in step 2 is dissolved in distilled water, with mass percent 1~10%
The aqueous solution of composite, deaeration, obtain the aqueous solution of the composite after deaeration；
Step 4: by the aqueous solution of the composite obtained in step 3, injection promotees in CO2 laser weld pipe mold, is placed into
Reacted in less than -20~-5 DEG C environment to gel forming；
Step 5, the rush CO2 laser weld pipe being molded in step 4 is placed on freeze dryer and freezed through 30~50 hours to remove it
In water.
Embodiment six
A kind of compound rush CO2 laser weld pipe, is made up of following components by weight：
A kind of preparation method for promoting CO2 laser weld pipe, comprises the following steps：
Step 1: the methanol suspension for the gelatin that mass percent is 1~10%, the methanol suspension of chitosan is respectively configured
Liquid, the methanol solution of hyaluronic acid, the methanol solution of the methanol suspension of fibroin and coupling agent；
It is Step 2: the methanol of the methanol suspension of gelatin in step 1, the methanol suspension of chitosan, hyaluronic acid is molten
The methanol solution blending of liquid, the methanol suspension of fibroin and coupling agent, is warming up to 60~90 DEG C, stirring reaction 1~6 hour, takes out
Filter, is washed with absolute methanol repeatedly, until washing not reacted coupling agent, after 50~70 DEG C are dried in vacuo 6~24 hours, is obtained
To composite；
Step 3: the composite obtained in step 2 is dissolved in distilled water, with mass percent 1~10%
The aqueous solution of composite, deaeration, obtain the aqueous solution of the composite after deaeration；
Step 4: by the aqueous solution of the composite obtained in step 3, injection promotees in CO2 laser weld pipe mold, is placed into
Reacted in less than -20~-5 DEG C environment to gel forming；
Step 5, the rush CO2 laser weld pipe being molded in step 4 is placed on freeze dryer and freezed through 30~50 hours to remove it
In water.
Embodiment seven
A kind of compound rush CO2 laser weld pipe, is made up of following components by weight：
A kind of preparation method for promoting CO2 laser weld pipe, comprises the following steps：
Step 1: the methanol suspension for the gelatin that mass percent is 1~10%, the methanol suspension of chitosan is respectively configured
Liquid, the methanol solution of hyaluronic acid, the methanol solution of the methanol suspension of fibroin and coupling agent；
It is Step 2: the methanol of the methanol suspension of gelatin in step 1, the methanol suspension of chitosan, hyaluronic acid is molten
The methanol solution blending of liquid, the methanol suspension of fibroin and coupling agent, is warming up to 60~90 DEG C, stirring reaction 1~6 hour, takes out
Filter, is washed with absolute methanol repeatedly, until washing not reacted coupling agent, after 50~70 DEG C are dried in vacuo 6~24 hours, is obtained
To composite；
Step 3: the composite obtained in step 2 is dissolved in distilled water, with mass percent 1~10%
The aqueous solution of composite, deaeration, obtain the aqueous solution of the composite after deaeration；
Step 4: by the aqueous solution of the composite obtained in step 3, injection promotees in CO2 laser weld pipe mold, is placed into
Reacted in less than -20~-5 DEG C environment to gel forming；
Step 5, the rush CO2 laser weld pipe being molded in step 4 is placed on freeze dryer and freezed through 30~50 hours to remove it
In water.
Embodiment eight
A kind of compound rush CO2 laser weld pipe, is made up of following components by weight：
A kind of preparation method for promoting CO2 laser weld pipe, comprises the following steps：
Step 1: the methanol suspension for the gelatin that mass percent is 1~10%, the methanol suspension of chitosan is respectively configured
Liquid, the methanol solution of hyaluronic acid, the methanol solution of the methanol suspension of fibroin and coupling agent；
It is Step 2: the methanol of the methanol suspension of gelatin in step 1, the methanol suspension of chitosan, hyaluronic acid is molten
The methanol solution blending of liquid, the methanol suspension of fibroin and coupling agent, is warming up to 60~90 DEG C, stirring reaction 1~6 hour, takes out
Filter, is washed with absolute methanol repeatedly, until washing not reacted coupling agent, after 50~70 DEG C are dried in vacuo 6~24 hours, is obtained
To composite；
Step 3: the composite obtained in step 2 is dissolved in distilled water, with mass percent 1~10%
The aqueous solution of composite, deaeration, obtain the aqueous solution of the composite after deaeration；
Step 4: by the aqueous solution of the composite obtained in step 3, injection promotees in CO2 laser weld pipe mold, is placed into
Reacted in less than -20~-5 DEG C environment to gel forming；
Step 5, the rush CO2 laser weld pipe being molded in step 4 is placed on freeze dryer and freezed through 30~50 hours to remove it
In water.
Embodiment nine
A kind of compound rush CO2 laser weld pipe, is made up of following components by weight：
A kind of preparation method for promoting CO2 laser weld pipe, comprises the following steps：
Step 1: the methanol suspension for the gelatin that mass percent is 1~10%, the methanol suspension of chitosan is respectively configured
Liquid, the methanol solution of hyaluronic acid, the methanol solution of the methanol suspension of fibroin and coupling agent；
It is Step 2: the methanol of the methanol suspension of gelatin in step 1, the methanol suspension of chitosan, hyaluronic acid is molten
The methanol solution blending of liquid, the methanol suspension of fibroin and coupling agent, is warming up to 60~90 DEG C, stirring reaction 1~6 hour, takes out
Filter, is washed with absolute methanol repeatedly, until washing not reacted coupling agent, after 50~70 DEG C are dried in vacuo 6~24 hours, is obtained
To composite；
Step 3: the composite obtained in step 2 is dissolved in distilled water, with mass percent 1~10%
The aqueous solution of composite, deaeration, obtain the aqueous solution of the composite after deaeration；
Step 4: by the aqueous solution of the composite obtained in step 3, injection promotees in CO2 laser weld pipe mold, is placed into
Reacted in less than -20~-5 DEG C environment to gel forming；
Step 5, the rush CO2 laser weld pipe being molded in step 4 is placed on freeze dryer and freezed through 30~50 hours to remove it
In water.
Embodiment ten
A kind of compound rush CO2 laser weld pipe, is made up of following components by weight：
A kind of preparation method for promoting CO2 laser weld pipe, comprises the following steps：
Step 1: the methanol suspension for the gelatin that mass percent is 1~10%, the methanol suspension of chitosan is respectively configured
Liquid, the methanol solution of hyaluronic acid, the methanol solution of the methanol suspension of fibroin and coupling agent；
It is Step 2: the methanol of the methanol suspension of gelatin in step 1, the methanol suspension of chitosan, hyaluronic acid is molten
The methanol solution blending of liquid, the methanol suspension of fibroin and coupling agent, is warming up to 60~90 DEG C, stirring reaction 1~6 hour, takes out
Filter, is washed with absolute methanol repeatedly, until washing not reacted coupling agent, after 50~70 DEG C are dried in vacuo 6~24 hours, is obtained
To composite；
Step 3: the composite obtained in step 2 is dissolved in distilled water, with mass percent 1~10%
The aqueous solution of composite, deaeration, obtain the aqueous solution of the composite after deaeration；
Step 4: by the aqueous solution of the composite obtained in step 3, injection promotees in CO2 laser weld pipe mold, is placed into
Reacted in less than -20~-5 DEG C environment to gel forming；
Step 5, the rush CO2 laser weld pipe being molded in step 4 is placed on freeze dryer and freezed through 30~50 hours to remove it
In water.
The zoopery process of the present invention：
Zoopery:We have chosen adult rat (200-250 grams of body weight), promote CO2 laser weld in shape memory to assess
Pipe repairs the performance of nerve.Rat is divided into 4 groups and receives functional assessment by us, A groups are sham-operation group, B groups be negative control group,
The patent of invention " one kind promotees CO2 laser weld pipe and its preparation method and application " that C groups are Application No. CN201610345822.2
Promote CO2 laser weld pipe, D groups are rush CO2 laser weld pipe of the invention.Rat is carried out intraperitoneal injection chloraldurate (0.5 milliliter/
100 grams) anaesthetized.Body hair after Rat Right is removed, 1 millimeter of wound parallel with femur is cut at sciatic nerve, by stock
Biceps separates, and exposes sciatic nerve.At furcal nerve 5mm, A groups do not cut nerve, and B groups suture after cutting nerve, C
Group cut neural bag Application No. CN201610345822.2 patent of invention " one kind promote CO2 laser weld pipe and preparation method thereof and
Using " rush CO2 laser weld pipe, D groups cut the rush CO2 laser weld pipe of the nerve bag present invention, then by sciatic nerve index and
The modes such as nerve conduction velocity detect the functional restoration for having the function that the rush CO2 laser weld pipe of shape memory to sciatic nerve,
Detection data were taken to draw following table respectively at 15 days, 30 days, 45 days, 60 days, 75 days and 90 days：
By analysis of experimental results, with the extension of time, the sciatic nerve index of the rat using two kinds of nerve channel groups
Sham-operation group is all become closer to nerve conduction velocity, but D groups use the experimental group of the rush CO2 laser weld pipe of the present invention
The lifting speed of nerve conduction velocity and static sciatic nerve index is significantly greater than C group experimental groups, the rush of this explanation present invention
CO2 laser weld pipe is got a promotion due to the improvement of material etc..
The better embodiment of this patent is explained in detail above, but this patent is not limited to above-mentioned embodiment party
Formula, can also be on the premise of this patent objective not be departed from one skilled in the relevant art's possessed knowledge
Various changes can be made.
Claims (9)
1. a kind of compound rush CO2 laser weld pipe, it is characterised in that the rush CO2 laser weld pipe is by weight by following components structure
Into：
50 ~ 100 parts of gelatin；
20 ~ 50 parts of chitosan；
10 ~ 25 parts of hyaluronic acid；
10 ~ 20 parts of fibroin；
0.2 ~ 1 part of coupling agent.
2. compound rush CO2 laser weld pipe according to claim 1, it is characterised in that the gelatin is medicinal bone gelatin.
3. compound rush CO2 laser weld pipe according to claim 1, it is characterised in that the coupling agent is EDC-HCl.
4. the compound preparation method for promoting CO2 laser weld pipe according to claim 1, it is characterised in that comprise the following steps：
Step 1: the methanol suspension of gelatin, the methanol suspension of chitosan that mass percent is 1 ~ 10%, thoroughly is respectively configured
Methanol solution, the methanol suspension of fibroin and the methanol solution of coupling agent of bright matter acid；
Step 2: by the methanol suspension of gelatin in step 1, the methanol suspension of chitosan, hyaluronic acid methanol solution,
The methanol suspension of fibroin and the methanol solution blending of coupling agent, are warming up to 60 ~ 90 DEG C, stirring reaction 1 ~ 6 hour, filter,
Washed with absolute methanol, until washing not reacted coupling agent, after 50 ~ 70 DEG C are dried in vacuo 6 ~ 24 hours, obtained repeatedly
Composite；
Step 3: the composite obtained in step 2 is dissolved in distilled water, with mass percent 1 ~ 10% it is compound
The aqueous solution of material, deaeration, obtain the aqueous solution of the composite after deaeration；
Step 4: by the aqueous solution of the composite obtained in step 3, injection promotees in CO2 laser weld pipe mold, is placed into -20
Reacted in less than ~ -5 DEG C environment to gel forming；
Step 5, it is lyophilized therein to remove through 30 ~ 50 hours that the rush CO2 laser weld pipe being molded in step 4 is placed on freeze dryer
Water.
5. the compound preparation method for promoting CO2 laser weld pipe according to claim 4, it is characterised in that in the step 4
It is that high printing opacity PC promotees CO2 laser weld pipe mold to promote CO2 laser weld pipe mold.
6. the compound preparation method for promoting CO2 laser weld pipe according to claim 4, it is characterised in that in the step 4
It is that metal promotees CO2 laser weld pipe mold to promote CO2 laser weld pipe mold.
7. the compound preparation method for promoting CO2 laser weld pipe according to claim 4, it is characterised in that in the step 4
Promote CO2 laser weld pipe mold to be made by 3D printing.
8. the compound preparation method for promoting CO2 laser weld pipe according to claim 4, it is characterised in that freeze in the step 5
Rush CO2 laser weld pipe after dry is compressed by mould, is compressed to a diameter of original 40 ~ 50%, is then expanded by mould
Hole, reaming to internal diameter increase to the 100 ~ 120% of former internal diameter.
9. the compound preparation method for promoting CO2 laser weld pipe according to claim 8, it is characterised in that the compression and reaming
Mould be made by 3D printing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710740413.7A CN107510863A (en) | 2017-08-25 | 2017-08-25 | A kind of compound rush CO2 laser weld pipe and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710740413.7A CN107510863A (en) | 2017-08-25 | 2017-08-25 | A kind of compound rush CO2 laser weld pipe and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107510863A true CN107510863A (en) | 2017-12-26 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111603608A (en) * | 2020-05-15 | 2020-09-01 | 南通大学 | Pullulan-hyaluronic acid-microfiber spinal cord scaffold and preparation method thereof |
| CN115591016A (en) * | 2022-11-08 | 2023-01-13 | 南通大学（Cn） | Nerve graft with oriented micro-channel and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1493404A1 (en) * | 2003-06-30 | 2005-01-05 | DePuy Mitek, Inc. | Scaffold for connective tissue repair |
| CN101837149A (en) * | 2010-06-18 | 2010-09-22 | 华中科技大学 | Anti-coagulation anti-bacterial biomedical material and preparation method thereof |
| CN101879098A (en) * | 2009-05-08 | 2010-11-10 | 翰林大学校产学协力团 | Artificial eardrum using silk protein and method of fabricating the same |
| EP2624874A1 (en) * | 2010-10-08 | 2013-08-14 | Board Of Regents, The University Of Texas | Anti-adhesive barrier membrane using alginate and hyaluronic acid for biomedical applications |
| CN103724657A (en) * | 2013-12-25 | 2014-04-16 | 同济大学 | Preparation method of crosslinked chitosan/gelatin composite porous scaffold |
| CN105879112A (en) * | 2016-04-21 | 2016-08-24 | 四川大学 | Neural restoration promoting tube as well as preparation method and application thereof |
| CN106913393A (en) * | 2015-12-28 | 2017-07-04 | 山东国际生物科技园发展有限公司 | A kind of artificial neuron support and preparation method and application |
-
2017
- 2017-08-25 CN CN201710740413.7A patent/CN107510863A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1493404A1 (en) * | 2003-06-30 | 2005-01-05 | DePuy Mitek, Inc. | Scaffold for connective tissue repair |
| CN101879098A (en) * | 2009-05-08 | 2010-11-10 | 翰林大学校产学协力团 | Artificial eardrum using silk protein and method of fabricating the same |
| CN101837149A (en) * | 2010-06-18 | 2010-09-22 | 华中科技大学 | Anti-coagulation anti-bacterial biomedical material and preparation method thereof |
| EP2624874A1 (en) * | 2010-10-08 | 2013-08-14 | Board Of Regents, The University Of Texas | Anti-adhesive barrier membrane using alginate and hyaluronic acid for biomedical applications |
| CN103724657A (en) * | 2013-12-25 | 2014-04-16 | 同济大学 | Preparation method of crosslinked chitosan/gelatin composite porous scaffold |
| CN106913393A (en) * | 2015-12-28 | 2017-07-04 | 山东国际生物科技园发展有限公司 | A kind of artificial neuron support and preparation method and application |
| CN105879112A (en) * | 2016-04-21 | 2016-08-24 | 四川大学 | Neural restoration promoting tube as well as preparation method and application thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111603608A (en) * | 2020-05-15 | 2020-09-01 | 南通大学 | Pullulan-hyaluronic acid-microfiber spinal cord scaffold and preparation method thereof |
| CN115591016A (en) * | 2022-11-08 | 2023-01-13 | 南通大学（Cn） | Nerve graft with oriented micro-channel and preparation method thereof |
| CN115591016B (en) * | 2022-11-08 | 2023-12-19 | 南通大学 | Nerve graft with orientation micro-channel and preparation method thereof |
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Application publication date: 20171226 |