CN107475161A - The technique for removing hydrogen sulfide contamination thing in house refuse using microorganism formulation - Google Patents

The technique for removing hydrogen sulfide contamination thing in house refuse using microorganism formulation Download PDF

Info

Publication number
CN107475161A
CN107475161A CN201710871885.6A CN201710871885A CN107475161A CN 107475161 A CN107475161 A CN 107475161A CN 201710871885 A CN201710871885 A CN 201710871885A CN 107475161 A CN107475161 A CN 107475161A
Authority
CN
China
Prior art keywords
atcc
microorganism formulation
technique
domestication
hydrogen sulfide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201710871885.6A
Other languages
Chinese (zh)
Other versions
CN107475161B (en
Inventor
黄灵君
周迪峰
吴法明
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HANGZHOU FUYANG JIACHANG MACHINERY Co.,Ltd.
Original Assignee
黄灵君
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 黄灵君 filed Critical 黄灵君
Priority to CN201710871885.6A priority Critical patent/CN107475161B/en
Publication of CN107475161A publication Critical patent/CN107475161A/en
Application granted granted Critical
Publication of CN107475161B publication Critical patent/CN107475161B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/46—Removing components of defined structure
    • B01D53/48—Sulfur compounds
    • B01D53/52—Hydrogen sulfide
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84—Biological processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2251/00—Reactants
    • B01D2251/95—Specific microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

The invention belongs to microbial technology field, discloses the technique that hydrogen sulfide contamination thing in house refuse is removed using microorganism formulation, it comprises the following steps：Microorganism formulation is added in the water of 10 times of weight, is uniformly mixing to obtain dilution；Rubbish is paved into 50cm thickness, then the jack on rubbish, aperture is 1 5mm, and hole density is 100 200/m2, then according to every m2Dilution is uniformly sprayed using the amount of 3 5kg dilutions, processing time is more than 24 hours.Present invention process simple possible, it is environment friendly and pollution-free.

Description

The technique for removing hydrogen sulfide contamination thing in house refuse using microorganism formulation
Technical field
The invention belongs to microbial technology field, and it is dirty more particularly to remove hydrogen sulfide in house refuse using microorganism formulation The technique for contaminating thing.
Background technology
With expanding economy and the raising of living standards of the people, stench has increasingly been closed as one of environmental hazard Note.A large amount of unpleasant waste gas can be produced in sewage treatment plant and much industry, agricultural production and in house refuse heap, wherein main It is sulfur-containing compound to cause smelly composition.These materials come that source electrode is wide, and toxicity is very big, wherein again with hydrogen sulfide it is most commonly seen, influence Also it is most wide.
Hydrogen sulfide stripping method has much, can simply be divided into wet desulphurization, dry desulfurization and biological desulphurization.Wet method Desulfurization is to remove a kind of method of hydrogen sulfide with hydrogen sulfide reaction by the specific solvent such as sodium hydroxide, ammoniacal liquor, passes through oxygen Effect of the gas to solvent reaches recycling for solvent.Due to the influence of the flow velocity and flow of sodium hydroxide, hydrogen sulfide can not It has been dissolved completely in wherein, and thiosulfate can be produced in course of dissolution, these will all influences desulfurized effect, and also have The problems such as investment is more, operational management is complicated, desulphurization cost is high and absorbing liquid need to be changed.Dry desulfurization is that one kind utilizes oxygen, with Oxidation of Hydrogen Sulfide is elemental sulfur or a kind of desulfurization method of sulfide as oxidant by iron oxide.Elemental sulfur is in absorption process In serve one catalysis effect.But desulfurization by dry method has, and installation area is big, operation is discontinuous, desulfurizing agent is not easy again Life, it is not easy to change the problem such as low with desulfuration efficiency.Biological desulfurizing technology is by H by microbial metabolism approach2S is converted into sulfuric acid A kind of removing sulfuldioxide of salt or elemental sulfur, have the advantages that operating cost it is low, it is less formation secondary pollution, it has also become both at home and abroad Odor prevention is studied and the main stream approach in application.But prior art is difficult to find the microbial inoculum that compatibility is reasonable, effect is good, big portion Point microbial inoculum exist can not symbiosis collaboration, cause deodorizing effect poor, do not reach the requirement of people.
The content of the invention
The defects of in order to overcome prior art, object of the present invention is to provide utilize microorganism formulation removing life rubbish The technique of hydrogen sulfide contamination thing in rubbish, the PROCESS FOR TREATMENT effect is good, environment friendly and pollution-free.
Above-mentioned purpose of the present invention is achieved by the following technical programs：
The technique that hydrogen sulfide contamination thing in house refuse is removed using microorganism formulation, it comprises the following steps：By microorganism system Agent is added in the water of 10 times of weight, is uniformly mixing to obtain dilution；Rubbish is paved into 50cm thickness, then inserted on rubbish Hole, aperture 1-5mm, hole density are 100-200/m2, then according to every m2Uniformly sprayed using the amount of 3-5kg dilutions dilute Liquid is released, processing time is more than 24 hours.
Specifically,
The preparation method of the microorganism formulation comprises the following steps：
Step 1）Prepare domestication culture medium, step 2）Bacterial strain domestication step, 3）Prepare carrier, step 4）Prepare microorganism formulation.
Further,
The microorganism formulation is prepared in accordance with the following steps：
Step 1）Prepare domestication culture medium：Take glucose 50g, dregs of beans 20g, wheat bran 10g, ammonium sulfate 5g, vulcanized sodium 5g, phosphoric acid Hydrogen dipotassium 1g, potassium dihydrogen phosphate 1g, ferrous sulfate 1g, is added in water, is settled to 1L, and domestication culture medium is made；
Step 2）Bacterial strain is tamed：By onion Burkholderia, enterococcus faecalis, Acidithiobacillus ferrooxidans strain GF, Candida albicans, glimmering Light pseudomonad, moist cellulomonas cartae obtain 1 × 10 according to cellar culture respectively8CFU/ml seed liquor, then according to 2: 3:3:5:5:7 volume ratio is mixed to get seed mixture liquid, is gone to according still further to 8% inoculum concentration in domestication culture medium, 28 DEG C of domestications 12h is cultivated, obtains taming bacterium solution；
Step 3）Prepare microbe carrier：By sawdust, manioc waste and turfy soil according to 5:5:3 mass ratio, which mixes, to be made；
Step 4）Prepare microorganism formulation：Domestication bacterium solution is added in the microbe carrier of 2 times of quality, 200rpm stirrings 3min, low temperature drying is then carried out, drying temperature is 20 DEG C, and water content is 6wt% after drying, and packaging, is produced.
Preferably,
The onion Burkholderia is ATCC 25416；The enterococcus faecalis is ATCC 29212；The acidophilus ferrous oxide sulphur Bacillus is ATCC 53993；The Candida albicans are ATCC 10231；The Pseudomonas fluorescens is ATCC 13525； The moist cellulomonas cartae is ATCC 491.
Further,
The preferred scheme of the above-mentioned simply present invention.As less preferred technical scheme, quantity of the present invention to bacterial strain in seed liquor Also have no particular limits, this can be needed according to the environment to determine.
Strain of the present invention belongs to known bacterial strain, can be commercially available from commercial sources such as ATCC.The present invention's The seed culture and fermented and cultured of each strain are the cellar culture mode of this area, are not innovative points of the present invention, are not described in detail herein. Raw material or reagent used in the present invention is commercially available in addition to special instruction.
Compared with prior art, the beneficial effect that the present invention obtains mainly includes but is not limited to following side Face：
Present invention process has used microorganism formulation, environment friendly and pollution-free, and cost is cheap, can effectively handle the sulfur-bearings such as hydrogen sulfide Malodorant, effect is good, can be applied to the removing of hydrogen sulfide in rubbish deodorization, biogas and oil well；
Reasonable compatibility between each strain in microorganism formulation of the present invention, symbiosis are coordinated, and mutually antagonism, activity be not high；Bacterium solution passes through first Domestication culture is crossed, environment can be more rapidly adapted to, improve desulfuration efficiency；
The present invention has used a large amount of discarded objects when preparing carrier, when not only saving cost, and can strengthen the survival of bacterial strain Between and colonization ability；Wherein, sawdust and manioc waste can adjust the water content and gas permeability of soil, can also supply nutrients, grass Charcoal soil water conservation is ventilated；Composite bacteria agent of the present invention is prepared using carrier and mixed bacteria liquid, and thalline adhesion effect is good, load capacity Greatly, specific surface area is big, can improve degradation speed；
The preparation technology of microorganism formulation of the present invention is simple, and cost is cheap, good deodorization effect, and ecological, environmental protective is easy to use, production Cost is low, to environment non-secondary pollution, has broad application prospects.
Brief description of the drawings
Fig. 1：Microorganism formulation handles the clearance of 24 hours hydrogen sulfide and foul smell；
Fig. 2：Microorganism formulation handles the clearance of 48 hours hydrogen sulfide and foul smell.
Embodiment
In order that those skilled in the art more fully understand the technical scheme in the application, have below in conjunction with the application Body embodiment, the technical scheme of the application is clearly and completely described, it is clear that described embodiment is only this Shen Please part of the embodiment, rather than whole embodiment.Based on the embodiment in the application, those of ordinary skill in the art are not having There is the every other embodiment made and obtained under the premise of creative work, should all belong to the scope of protection of the invention.
Embodiment 1
The technique that hydrogen sulfide contamination thing in house refuse is removed using microorganism formulation, it comprises the following steps：By microorganism system Agent is added in the water of 10 times of weight, is uniformly mixing to obtain dilution；Rubbish is paved into 50cm thickness, then inserted on rubbish Hole, aperture 2mm, hole density are 200/m2, then according to every m2Dilution is uniformly sprayed using the amount of 3kg dilutions, is handled Time is 24 hours.
The preparation method of the microorganism formulation comprises the following steps：
Step 1）Prepare domestication culture medium：Take glucose 50g, dregs of beans 20g, wheat bran 10g, ammonium sulfate 5g, vulcanized sodium 5g, phosphoric acid Hydrogen dipotassium 1g, potassium dihydrogen phosphate 1g, ferrous sulfate 1g, is added in water, is settled to 1L, and domestication culture medium is made；
Step 2）Bacterial strain is tamed：By onion Burkholderia, enterococcus faecalis, Acidithiobacillus ferrooxidans strain GF, Candida albicans, glimmering Light pseudomonad, moist cellulomonas cartae obtain 1 × 10 according to cellar culture respectively8CFU/ml seed liquor, then according to 2: 3:3:5:5:7 volume ratio is mixed to get seed mixture liquid, is gone to according still further to 8% inoculum concentration in domestication culture medium, 28 DEG C of domestications 12h is cultivated, obtains taming bacterium solution；
Step 3）Prepare microbe carrier：By sawdust, manioc waste and turfy soil according to 5:5:3 mass ratio, which mixes, to be made；
Step 4）Prepare microorganism formulation：Domestication bacterium solution is added in the microbe carrier of 2 times of quality, 200rpm stirrings 3min, low temperature drying is then carried out, drying temperature is 20 DEG C, and water content is 6wt% after drying, and packaging, is produced.
The onion Burkholderia is ATCC 25416；The enterococcus faecalis is ATCC 29212；The acidophilus oxidation is sub- Iron Thiobacillus is ATCC 53993；The Candida albicans are ATCC 10231；The Pseudomonas fluorescens is ATCC 13525；The moist cellulomonas cartae is ATCC 491.
Embodiment 2
The technique that hydrogen sulfide contamination thing in house refuse is removed using microorganism formulation, it comprises the following steps：By microorganism system Agent is added in the water of 10 times of weight, is uniformly mixing to obtain dilution；Rubbish is paved into 50cm thickness, then inserted on rubbish Hole, aperture 4mm, hole density are 100/m2, then according to every m2Dilution is uniformly sprayed using the amount of 4kg dilutions, is handled Time is 48 hours.
The preparation method of the microorganism formulation comprises the following steps：
Step 1）Prepare domestication culture medium：Take glucose 50g, dregs of beans 20g, wheat bran 10g, ammonium sulfate 5g, vulcanized sodium 5g, phosphoric acid Hydrogen dipotassium 1g, potassium dihydrogen phosphate 1g, ferrous sulfate 1g, is added in water, is settled to 1L, and domestication culture medium is made；
Step 2）Bacterial strain is tamed：By onion Burkholderia, enterococcus faecalis, Acidithiobacillus ferrooxidans strain GF, Candida albicans, glimmering Light pseudomonad, moist cellulomonas cartae obtain 1 × 10 according to cellar culture respectively8CFU/ml seed liquor, then according to 2: 3:3:5:5:7 volume ratio is mixed to get seed mixture liquid, is gone to according still further to 8% inoculum concentration in domestication culture medium, 28 DEG C of domestications 12h is cultivated, obtains taming bacterium solution；
Step 3）Prepare microbe carrier：By sawdust, manioc waste and turfy soil according to 5:5:3 mass ratio, which mixes, to be made；
Step 4）Prepare microorganism formulation：Domestication bacterium solution is added in the microbe carrier of 2 times of quality, 200rpm stirrings 3min, low temperature drying is then carried out, drying temperature is 20 DEG C, and water content is 6wt% after drying, and packaging, is produced.
The onion Burkholderia is ATCC 25416；The enterococcus faecalis is ATCC 29212；The acidophilus oxidation is sub- Iron Thiobacillus is ATCC 53993；The Candida albicans are ATCC 10231；The Pseudomonas fluorescens is ATCC 13525 ；The moist cellulomonas cartae is ATCC 491.
Embodiment 3
Laboratory stage detects the removal effect of hydrogen sulfide：
First by 50ml fluid nutrient mediums（Formula does not add vulcanized sodium with domestication culture medium）It is placed in 250ml blake bottles, The water that microorganism formulation in embodiment 1 adds 10 times of weight is diluted, is then inoculated into fluid nutrient medium according to 10% inoculum concentration In, 25ml pure hydrogen sulfides are passed through, respectively in the concentration of 24h, 48h detection hydrogen sulfide；Blank control is set simultaneously（Do not add Microorganism formulation）；Sub-methyl blue spectrum analysis determines the concentration (g/m of hydrogen sulfide3), specific testing result is shown in Table 1：
Table 1
Group Initial concentration 24h 48h
Embodiment 1 3.982 0.763 0.018
Blank control 4.017 3.914 3.875
Conclusion：Laboratory small scale experiments find that microorganism formulation of the present invention can effectively absorb removal hydrogen sulfide gas, The clearance of 48h hours reaches more than 99%.
Embodiment 4
Domestic rubbish disposal effect test：
By 8 tons of corrupt house refuses, 8 parts are divided into, 1 ton every part, is respectively placed in equirotal 8 closed rooms, Respectively experimental group（1 group of embodiment）, vehicle Control group（Only with carrier, microbial inoculum is not added）, remaining is the same as embodiment 1；Control Group 1：Onion Burkholderia is not added, and remaining is the same as embodiment 1；Control group 2：Enterococcus faecalis is not added, remaining is the same as embodiment 1；It is right According to group 3：Acidithiobacillus ferrooxidans strain GF is not added, remaining is the same as embodiment 1；Control group 4：Candida albicans are not added, remaining With embodiment 1；Control group 5：Pseudomonas fluorescens is not added, remaining is the same as embodiment 1；Control group 6：Moist fiber unit cell is not added Bacterium, remaining is the same as embodiment 1；
Processing method：With reference to the method for embodiment 1, respectively at the different acquisition point sampling of 24h, 48h around rubbish, measure H2S clearances and foul smell clearance.H2S uses ammonium alcohol polyvinyl phosphate absorption-methylene-blue colorimetric method.Odor concentration uses XP-329IIIR odorousnesses detector measures.
Interpretation of result：As shown in figure 1, processing time is 24h, test group H2S and foul smell clearance are 80% or so, and carrier pair It is only 10% or so according to group, the treatment effect of test group is substantially better than vehicle Control group, also superior to control group 1-6；As shown in Fig. 2 Processing time is 48h, test group H2S and foul smell clearance are more than 90%, and treatment effect is substantially better than control group, prompts this hair Bright microorganism formulation compatibility is reasonable, and collaboration symbiosis effect is good.
Although above the present invention is described in detail with a general description of the specific embodiments, in this hair On the basis of bright, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore, exist Without departing from these modifications or improvements on the basis of spirit of the invention, the scope of protection of present invention is belonged to.

Claims (7)

1. for the technique using hydrogen sulfide contamination thing in microorganism formulation removing house refuse, it comprises the following steps：Will be micro- Biological agent is added in the water of 10 times of weight, is uniformly mixing to obtain dilution；Rubbish is paved into 50cm thickness, then in rubbish Upper jack, aperture 1-5mm, hole density are 100-200/m2, then according to every m2Uniformly sprayed using the amount of 3-5kg dilutions Dilution is spilt, processing time is more than 24 hours.
2. technique according to claim 1, it is characterised in that the preparation method of the microorganism formulation includes following step Suddenly：Step 1）Prepare domestication culture medium, step 2）Bacterial strain domestication step, 3）Prepare carrier, step 4）Prepare microorganism formulation.
3. technique according to claim 2, it is characterised in that the step 1）Prepare domestication culture medium, including following step Suddenly：Glucose 50g, dregs of beans 20g, wheat bran 10g, ammonium sulfate 5g, vulcanized sodium 5g, dipotassium hydrogen phosphate 1g, potassium dihydrogen phosphate 1g are taken, Ferrous sulfate 1g, is added in water, is settled to 1L, and domestication culture medium is made.
4. according to the technique described in claim 2-3, it is characterised in that the step 2）Bacterial strain is tamed, and is comprised the following steps：Will Onion Burkholderia, enterococcus faecalis, Acidithiobacillus ferrooxidans strain GF, Candida albicans, Pseudomonas fluorescens, moist fiber list Born of the same parents bacterium obtains 1 × 10 according to cellar culture respectively8CFU/ml seed liquor, then according to 2:3:3:5:5:7 volume ratio mixing Seed mixture liquid is obtained, is gone to according still further to 8% inoculum concentration in domestication culture medium, 28 DEG C of domestication culture 12h, obtains taming bacterium solution.
5. according to the technique described in claim 2-4, it is characterised in that the step 3）Microbe carrier is prepared, including it is as follows Step：By sawdust, manioc waste and turfy soil according to 5:5:3 mass ratio, which mixes, to be made.
6. according to the technique described in claim 2-5, it is characterised in that the step 4）Microorganism formulation is prepared, including it is as follows Step：Domestication bacterium solution is added in the microbe carrier of 2 times of quality, 200rpm stirring 3min, low temperature drying is then carried out, does Dry temperature is 20 DEG C, and water content is 6wt% after drying, and packaging, is produced.
7. according to the technique described in claim 2-6, it is characterised in that the onion Burkholderia is ATCC 25416；It is described Enterococcus faecalis is ATCC 29212；The Acidithiobacillus ferrooxidans strain GF is ATCC 53993；The Candida albicans are ATCC 10231；The Pseudomonas fluorescens is ATCC 13525；The moist cellulomonas cartae is ATCC 491.
CN201710871885.6A 2017-09-25 2017-09-25 Process for removing hydrogen sulfide pollutants in household garbage by using microbial preparation Active CN107475161B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710871885.6A CN107475161B (en) 2017-09-25 2017-09-25 Process for removing hydrogen sulfide pollutants in household garbage by using microbial preparation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710871885.6A CN107475161B (en) 2017-09-25 2017-09-25 Process for removing hydrogen sulfide pollutants in household garbage by using microbial preparation

Publications (2)

Publication Number Publication Date
CN107475161A true CN107475161A (en) 2017-12-15
CN107475161B CN107475161B (en) 2021-07-20

Family

ID=60586772

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710871885.6A Active CN107475161B (en) 2017-09-25 2017-09-25 Process for removing hydrogen sulfide pollutants in household garbage by using microbial preparation

Country Status (1)

Country Link
CN (1) CN107475161B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110499262A (en) * 2018-05-17 2019-11-26 卢松 Purification absorbs the compound of slurry-spraying pelletizing flue gas
CN112126602A (en) * 2020-09-27 2020-12-25 归尚(上海)新能源科技有限公司 Biogas in-situ desulfurization biological agent

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101205101A (en) * 2007-12-13 2008-06-25 武汉工业学院 Treatment method of oil-containing waste water
WO2012160526A2 (en) * 2011-05-23 2012-11-29 Ofir Menashe Formulations of microorganism comprising particles and uses of same
CN103865847A (en) * 2014-03-06 2014-06-18 深圳市三林生物科技工程有限公司 Composite microbial preparation for garbage deodorization and preparation method thereof
CN105000923A (en) * 2015-07-10 2015-10-28 广东省微生物研究所 Bio-organic fertilizer of poultry feather amino acid and preparation method of same
CN104528955B (en) * 2015-01-22 2016-08-24 曹俊萍 A kind of microorganism formulation for repairing chemical fertilizer factory's sewage

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101205101A (en) * 2007-12-13 2008-06-25 武汉工业学院 Treatment method of oil-containing waste water
WO2012160526A2 (en) * 2011-05-23 2012-11-29 Ofir Menashe Formulations of microorganism comprising particles and uses of same
CN103865847A (en) * 2014-03-06 2014-06-18 深圳市三林生物科技工程有限公司 Composite microbial preparation for garbage deodorization and preparation method thereof
CN104528955B (en) * 2015-01-22 2016-08-24 曹俊萍 A kind of microorganism formulation for repairing chemical fertilizer factory's sewage
CN105000923A (en) * 2015-07-10 2015-10-28 广东省微生物研究所 Bio-organic fertilizer of poultry feather amino acid and preparation method of same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CLAUDIO PAGELLA等: "Biotechnological H2S gas treatment with Thiobacillus ferrooxidans", 《CHEM. ENG. TECHNOL.》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110499262A (en) * 2018-05-17 2019-11-26 卢松 Purification absorbs the compound of slurry-spraying pelletizing flue gas
CN112126602A (en) * 2020-09-27 2020-12-25 归尚(上海)新能源科技有限公司 Biogas in-situ desulfurization biological agent

Also Published As

Publication number Publication date
CN107475161B (en) 2021-07-20

Similar Documents

Publication Publication Date Title
CN107189972A (en) Microbial deodorant, preparation method and the usage
CN105420147A (en) Compound microbial preparation for treating black and odorous rivers through strengthened calcium nitrate
CN103864483B (en) Microorganism chelating agent for producing bio-organic fertilizer by removing organic solid waste heavy metal
CN104399098A (en) Composite microbial deodorant and preparation method thereof
CN110358699B (en) Compound microbial deodorant bacterial agent and preparation method and application thereof
CN101289637B (en) Method for preparing clean fuel derived from sludge
CN104258440A (en) Biological deodorant and preparation method thereof
CN107557318A (en) For removing the microorganism formulation of hydrogen sulfide malodorant
CN102327630A (en) Microbial strain and deodorant for municipal landfill, and preparation and application method thereof
CN101624300B (en) Method for removing heavy metal in solid organic waste compost
CN109593663A (en) A kind of high-performance bio desulfurization microbial inoculum and its application method
CN107475161A (en) The technique for removing hydrogen sulfide contamination thing in house refuse using microorganism formulation
CN102093098A (en) Method for preparing fertilizer by using domestic sludge
CN105585235A (en) Compound microbial preparation with deodorization function, preparation method and application
CN105536507B (en) A kind of deodorizing method for washing deodorizing tower and its coordinating microbial deodorant
CN110591975A (en) Immobilized thermophilic microbial community for treating municipal sludge and preparation and use methods thereof
CN110093292A (en) A kind of composite biological agent and preparation method and processing method for handling domestic sludge
CN109331641A (en) A kind of preparation method of garbage deodorant
CN103922482A (en) Deodorizing liquid based on compound microorganisms and growth promoters thereof
CN103272477A (en) Compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application thereof
CN112625945A (en) Compound microorganism deodorization bacterium and preparation method thereof
CN112725244A (en) Efficient deodorant microbial inoculum for stink of potato starch wastewater
CN109971650A (en) It is a kind of for removing the biological agent of slurry-spraying pelletizing tail gas peculiar smell
CN112251357A (en) Method for screening livestock and poultry manure fermentation microbial inoculum
CN109158403A (en) House refuse biology high temperature drying method for treating

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20210701

Address after: 311400 Xindeng town Wuliqiao village, Fuyang District, Hangzhou City, Zhejiang Province

Applicant after: HANGZHOU FUYANG JIACHANG MACHINERY Co.,Ltd.

Address before: 311400 Xiankou village, Luzhu Town, Fuyang District, Hangzhou City, Zhejiang Province (Hangzhou Fuyang Liangyuan Ecological Agriculture Development Co., Ltd.)

Applicant before: Huang Lingjun

GR01 Patent grant
GR01 Patent grant