CN107449907B - A kind of the lateral flow chromatography detection card and detection method of encryption - Google Patents
A kind of the lateral flow chromatography detection card and detection method of encryption Download PDFInfo
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Abstract
Detection card is chromatographed the invention discloses a kind of lateral flow of encryption and detection method, main feature are as follows: being blocked upper in a detection while being integrated multiple lateral flow chromatography sense channels；Detection species corresponding to each channel put in order with randomness, and user can not direct naked eyes interpretation testing result.Every detection card has unique identification code, and the identity for card identifies, the solution confidential information comprising each sense channel.Chart scanner sends network server by internet for the original detection signal of identification code and each channel and is decrypted, and detection data is back to chart scanner display output by network.
Description
Technical field
The invention belongs to bioanalysis detection field, a kind of lateral flow chromatography detection card more particularly, to encryption and
Detection method.
Background technique
Immune chromatography test card is a kind of rapid immunoassay method for combining immunological technique and chromatographic techniques,
Principle is: special antibody being first fixed on to a certain zone of nitrocellulose filter, when nitrocellulose one end of the drying is soaked
After entering sample, due to capillarity, sample will be moved forward along the film, when being moved to the region for being fixed with antibody, sample
Corresponding antigen is specifically bound with the antibody in product, if the region can be made aobvious with immune colloid gold or Immunoperoxidase Staining
Show certain color, or magnetic particle is used the region detection can be made to go out magnetic signal as label, to realize exempting from for specificity
Epidemic disease diagnosis.Wherein, the immunochromatography technique of colloid gold label have it is simple and quick, as a result accurately, high sensitivity the characteristics of, application
It the most extensively, is a kind of Fast Detection Technique risen in recent years.
With the development of technology and the market demand, the qualitative analysis that immune chromatography test card is also identified from the naked eyes of early stage,
It develops into present chart scanner and carries out quantitative analysis.A kind of immuno-chromatographic test paper strip inspection is just mentioned in patent CN104914239A
Measurement equipment carries out Image Acquisition by picture pick-up device, and microprocessor and other hardware circuits realize quantitative detection and show knot
Fruit.The appearance of chart scanner not only promotes the accuracy and efficiency of detection, but also further provides for the preservation and management of data
Convenience.A kind of colloidal gold immunochromatographimethod of combination data transmission of internet of things system is just referred in patent CN103499687A
Instrument saves detection data and is analyzed in conjunction with data transmission of internet of things system, convenient for carrying out to detection data
Retrospective analysis.
But, chromatography detection card currently on the market is mostly lateral flow design, and the either direct interpretation of test card obtains fixed
Property testing result, or by chart scanner sxemiquantitative or quantitative analysis as a result, user can naked eyes according to survey card on
Detection line (T line) and nature controlling line (C line) whether develop the color to judge that testing result is negative or positive.So, even if
It is layman, can finds out testing result by naked eyes, there is the risks of artificial garbled data.Certain tested units,
For number one, base testing staff may be bribed, testing result is conceal in false.On the other hand, certain tested units are
The supervision for escaping government department provides false sample to be detected, such as directly with plain boiled water or water mixing, this will also lead
Cause detection data untrue.
Summary of the invention
Aiming at the above defects or improvement requirements of the prior art, the present invention provides a kind of lateral flow of encryption chromatographies to detect
Card and detection mode, its object is to solve current immunochromatographydetection detection card naked eyes to can be seen that data caused by testing result are conceal
Fail to report problem and the untrue caused data invalidity problem of sample.
According to one aspect of the present invention, a kind of lateral flow chromatography detection card of encryption is provided, which includes adding
Sample hole (1), sense channel (2), auxiliary sense channel (3) and identification code (4)；The well (1) be located at sense channel (2) and
Assist the side of sense channel (3)；The identification code (4) records each sense channel (2) and assists the solution of sense channel (3)
Confidential information；The sense channel (2) and auxiliary sense channel (3) are chromatography sense channel；The sense channel (2) is at least
1；The auxiliary sense channel (3) determines that the method for testing result is different from sense channel (2)；The sense channel (2) and
It is random for assisting putting in order for sense channel (3).
Preferably, when the sense channel (2) are more than or equal to 2, the sense channel (2) for detecting respectively simultaneously
Different testing sample matter.
Preferably, the sense channel (2) determines testing result using competition law, and the auxiliary sense channel (3) uses
Double-antibody method determines testing result；Or the sense channel (2) determines testing result, the auxiliary using double-antibody method
Sense channel (3) determines testing result using competition law.
Preferably, the sense channel (2) is colloidal gold immunochromatographimethod channel, latex chromatography channel, magnetic particle chromatography
At least one of channel and nucleic acid chromatography sense channel；The identification code (4) is bar code, two dimensional code or NFC electronics mark
Label.
Preferably, the corresponding detection substance of auxiliary sense channel (3) is the intrinsic ingredient of determinand, the proper constituent
It can be used as the foundation for judging determinand sample authenticity, be the ingredient that sample itself has, be used for judgement sample authenticity；It is preferred that
Ground, the sense channel (2) are that the gold-marking immunity of penicillin chromatographs sense channel；The auxiliary sense channel (3) is cow's milk iron
The gold-marking immunity of albumen chromatographs sense channel, and bovine lactoferrin is ingredient intrinsic in milk itself, and presence or absence can be used for
Judge the determinand sample i.e. authenticity of milk.
Preferably, the solution confidential information that the identification code (4) is recorded is the corresponding detection substance letter in each channel of detection card
Breath；Either unique ID of detection card obtains each Air conduct measurement material information by networking.
Preferably, the sense channel (2) is that the latex of swine fever virus chromatographs sense channel, the auxiliary sense channel
(3) sense channel is chromatographed for the latex of heavy metal lead；Or the sense channel (2) is logical for the latex chromatography detection of heavy metal lead
Road, auxiliary sense channel (3) are that the latex of swine fever virus chromatographs sense channel.
Preferably, the sense channel (2) is the sense channel of Bt toxoprotein gene；Auxiliary sense channel (3)
Nature controlling line has rice phosphoric acid sucrose synthase gene probe；The detection line of auxiliary sense channel (3) includes two kinds of situations,
It is with rice phosphoric acid sucrose synthase gene probe either blank respectively.
Preferably, the sense channel (2) has three, is that Clenbuterol magnetic particle chromatography sense channel, Lake are more respectively
Bar amine magnetic particle chromatography sense channel and salbutamol magnetic particle chromatograph sense channel；The Quality Control of auxiliary sense channel (3)
Line has Clenbuterol Test paper；The detection line of auxiliary sense channel (3) includes two kinds of situations, is to have second respectively
Antibody either blank.Another aspect according to the invention provides a kind of lateral flow chromatography detection card with encryption
Detection method, include the following steps:
(1) a certain amount of sample is added dropwise into the well of detection card, stands chromatography and obtains detection card to be measured；
(2) the detection card insertion that sample has been added dropwise is entered into specific chart scanner and carries out interpretation.Chart scanner can read identification code with
And the detection signal in each channel, network server is sent collectively to by internet and is remotely handled；
(3) network server parses according to the validity of identification code and obtains the corresponding sense channel information of the detection card, from
It is dynamic to analyze the corresponding testing result of each sense channel, then the final detection result after decryption is recorded, and pass through interconnection
It nets defeated back to chart scanner；
(4) chart scanner receives the final detection result returned by network server, completes display, printing.
It is had the advantages that using technical solution of the present invention:
Species to be measured corresponding to multiple sense channels are random distributions, can not directly naked eyes recognition detection as a result,
Can only remotely be parsed by network server could obtain.When tested unit sees testing result, testing result is automatic
It is recorded on network server, such user can not just make a false report, fail to report, and avoid the risk of artificial garbled data.Pass through simultaneously
Auxiliary sense channel is added, judgement sample authenticity solves the problems, such as that sample is false.The upload of detection data, both avoided
Previous hand-kept it is possible that fault, the management and analysis of data can also be further realized, facilitate relevant departments
Control and supervision.
Detailed description of the invention
Fig. 1 is the lateral flow chromatography detection card schematic diagram of encryption provided by the invention.
Fig. 2 is the lateral flow chromatography detection card schematic diagram of encryption described in embodiment 4.
In all the appended drawings, identical appended drawing reference is used to denote the same element or structure, in which: 1- well；2-
Sense channel；3- assists sense channel；4- identification code；5- sampling head.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that described herein, specific examples are only used to explain the present invention, not
For limiting the present invention.As long as in addition, technical characteristic involved in the various embodiments of the present invention described below that
Not constituting conflict between this can be combined with each other.
The following are embodiments:
Embodiment 1
The present embodiment is related to a kind of encryption lateral flow gold-marking immunity chromatography quickly detected for Residues in Milk penicillin
Detection card and detection mode.
The lateral flow gold-marking immunity chromatography detection card of encryption: 2 sense channels are integrated on a card, are penicillin respectively
Gold-marking immunity chromatography sense channel and bovine lactoferrin gold-marking immunity chromatograph sense channel, the wherein detection of bovine lactoferrin
Authenticity for judgement sample.Two sense channel sequences are upset at random.Two dimensional code detection for identification is sticked in side
Card, as shown in Figure 1.
It is used for quickly detecting using the lateral flow chromatography detection card of encryption, comprising the following steps:
(1) 150 μ L milk samples to be measured are added dropwise into the well of detection card, stands chromatography and obtains chromatography to be measured in 15 minutes
Detection card.
The colloidal gold immunochromatographimethod detection of penicillin uses competition law, and when T line and C line develop the colors, result is negative；Work as T
Line does not develop the color, and C line develops the color, and testing result is the positive；If C line does not develop the color, for dead card.The detection of bovine lactoferrin uses
Double-antibody method, when T line and C line develop the color, result is the positive；When T line does not develop the color, and C line develops the color, and testing result is feminine gender；
If C line does not develop the color, for dead card.Therefore, no matter test sample penicillin is positive or feminine gender, except dead card, mould
C line on element and bovine lactoferrin sense channel always develops the color.If penicillin is the positive, the T line of penicillin sense channel is not
Colour developing, and the T line of bovine lactoferrin develops the color；If penicillin is feminine gender, the T line colour developing of penicillin sense channel, bovine lactoferrin
T line colour developing.The testing result of the two is mutually obscured, and the detection knot of determinand penicillin directly can not be directly judged from detection card
Fruit is negative or positive.
(2) chromatography to be measured detection card insertion is entered into chart scanner, chart scanner can read the original of two dimensional code and 2 sense channels
Signal is detected, network server is sent collectively to by internet and is remotely decrypted.
(3) network server parses according to the validity of identification code and obtains the corresponding sense channel information of the detection card, from
It is dynamic to analyze the corresponding testing result of each sense channel, it is then that the final detection result after decryption is defeated back to card reading by internet
Instrument.
If the T line of bovine lactoferrin sense channel develops the color, then testing result is the positive, show that sample is very, data can
It leans on；If the T line of bovine lactoferrin sense channel does not develop the color, then testing result is feminine gender, show that sample is false.
(4) chart scanner receives the final result returned by network server: the T line of penicillin sense channel does not develop the color, C line
Colour developing；Bovine lactoferrin sense channel C line and T line develop the color.Testing result is that sample is effective, and penicillin is negative.Completion display,
Printing.
Embodiment 2
The present embodiment is related to a kind of for combining the encryption lateral flow cream that swine fever quickly detects and heavy metal lead quickly detects
Glue-line analysis detection card and detection mode.
The lateral flow latex chromatography detection card of encryption: block at one upper while integrating 2 sense channels, be hog cholera respectively
The latex chromatography sense channel of poison and the latex of heavy metal lead chromatograph sense channel.Two sense channel sequences are upset at random.?
Bar code detection card for identification is sticked in side, as shown in Figure 2.
It is used for quickly detecting using the lateral flow chromatography detection card of encryption, comprising the following steps:
(1) 150 μ L Swine serums to be measured are added dropwise into the well of detection card, stands chromatography and obtains within 15 minutes chromatography inspection to be measured
Survey card.
The latex chromatography detection of swine fever virus uses double-antibody method, and when T line and C line develop the colors, result is positive；Work as T
Line does not develop the color, and C line develops the color, and testing result is feminine gender；If C line does not develop the color, for dead card.The detection of heavy metal lead is using competing
Method is striven, result is feminine gender when T line and C line develop the color；When T line does not develop the color, and C line develops the color, and testing result is the positive；If C line
It does not develop the color, is then dead card.Therefore, in the case where both effectively cards, if two T lines all develop the color or do not develop the color,
Show swine fever virus and heavy metal lead to have one be negative one to be positive；If a T line colour developing, one is not developed the color, and shows pig
Pestivirus and heavy metal lead are all negative or positive.The testing result of the two is mutually obscured, and can not visually directly sentence from detection card
It is disconnected.To realize encryption function.
(2) chromatography to be measured detection card insertion is entered into chart scanner, chart scanner can read the original of bar code and 2 sense channels
Signal is detected, network server is sent collectively to by internet and is remotely decrypted.
(3) network server parses according to the validity of identification code and obtains the corresponding sense channel information of the detection card, from
It is dynamic to analyze the corresponding testing result of each sense channel, it is then that the final detection result after decryption is defeated back to card reading by internet
Instrument.
(4) chart scanner receives the final detection result returned by network server: swine fever virus sense channel T line is not shown
Color, the colour developing of C line.Heavy metal lead sense channel T line does not develop the color, the colour developing of C line.Testing result is swine fever virus, negative；Heavy metal
Lead, it is positive.Complete display, printing.
Embodiment 3
The present embodiment is related to a kind of test card and detection mode for genetically modified crops detection, is particularly used for rice
The encryption lateral flow nucleic acid chromatography detection card and detection mode that middle Bt toxoprotein gene quickly detects.
The lateral flow nucleic acid chromatography detection card of encryption: block at one upper while integrating 2 sense channels, be Bt poison egg respectively
The nucleic acid chromatography sense channel and auxiliary chromatography sense channel of white gene.
The sense channel of Bt toxoprotein gene is the molecular probe of Bt toxoprotein gene at T line, is SPS gene at C line
(rice phosphoric acid sucrose synthase gene, all exist in all rice) molecular probe.Auxiliary chromatography sense channel is divided into two kinds:
One is be SPS gene molecule probe at T line and C line；One is blank at T line, only there is SPS gene molecule at C line
The sense channel of probe.If assist chromatography sense channel with the first, T line and C line develop the color；If being assisted with second
When chromatographing sense channel, only C line develops the color, and T line does not develop the color.Two kinds of auxiliary chromatography sense channels are matched at random with sense channel
It closes and uses, realize that testing result with the naked eye cannot directly determine, realize the purpose of testing result encryption.Bar shaped on detection card
Code describes the detection material information in each channel.
It is used for quickly detecting using the lateral flow chromatography detection card of encryption, comprising the following steps:
(1) 150 μ L Swine serums to be measured are added dropwise into the well of detection card, stands chromatography and obtains within 15 minutes chromatography inspection to be measured
Survey card.
The detection of Bt toxoprotein gene: when T line and C line develop the color, result is the positive；When T line does not develop the color, and C line is aobvious
Color, testing result are feminine gender；If C line does not develop the color, for dead card.Except dead card, the testing result of sense channel is assisted to have
Two kinds of situations: T and C line colour developing；The colour developing of C line and T line do not develop the color, since two kinds of card stochastic productions use, then the result occurred
It is also random.Virtual channel testing result and Bt toxoprotein gene testing result are mutually obscured, user just can not directly from
Detection card judgement, to realize encryption function.
(2) chromatography to be measured detection card insertion is entered into chart scanner, chart scanner can read the original of bar code and 2 sense channels
Signal is detected, network server is sent collectively to by internet and is remotely decrypted.
(3) network server parses according to the validity of identification code and obtains the corresponding sense channel information of the detection card, from
It is dynamic to analyze the corresponding testing result of each sense channel, it is then that the final detection result after decryption is defeated back to card reading by internet
Instrument.
(4) chart scanner receives the final result returned by network server: Bt genetic test channel C line and T line are not shown
Color；Auxiliary sense channel C line and T line develop the color.Testing result is Bt gene, negative.Complete display, printing.
Embodiment 4
The present embodiment is related to a kind of lateral flow magnetic particle chromatography detection for the encryption of clenbuterol hydrochloride joint-detection in pig urine
Card and detection mode.
The lateral flow magnetic particle chromatography detection card of encryption: block at one upper while integrating 4 sense channels, be Ke Lun respectively
Special sieve magnetic particle chromatography sense channel, Ractopamine magnetic particle chromatography sense channel, the chromatography detection of salbutamol magnetic particle are logical
Road and auxiliary sense channel.Auxiliary chromatography sense channel be divided into two kinds: one is at C line and T line solidification have secondary antibody,
One is blank at T line, solidification has secondary antibody at C line.If assist chromatography sense channel with the first, T line and C line are equal
Colour developing；If chromatograph sense channel with second of auxiliary, only C line develops the color, and T line does not develop the color.By two kinds of auxiliary chromatography inspections
It surveys channel to be used cooperatively at random with sense channel, realizes that testing result with the naked eye cannot directly determine, realize testing result and add
Close purpose.Bar code on detection card describes the detection material information in each channel.
3 sense channels and an accessory channel sequence are upset at random, stick NFC electronic identification label on detection card
The detection card for identification, as shown in Figure 2.
It is used for quickly detecting using the lateral flow chromatography detection card of encryption, comprising the following steps:
(1) sampling head that will test card is inserted into pig urine sample to be checked, is stood chromatography and is obtained within 15 minutes chromatography detection to be measured
Card.
The detection of Clenbuterol magnetic particle chromatography, the detection of Ractopamine magnetic particle chromatography and the chromatography inspection of salbutamol magnetic particle
Survey is all made of competition law.When T line and C line develop the color, result is feminine gender；When T line does not develop the color, and C line develops the color, and testing result is
It is positive；If C line does not develop the color, in vain.Except dead card, sense channel result is assisted to be divided into the colour developing of two kinds: T and C line；C line is aobvious
Color and T line do not develop the color, since two kinds of card stochastic productions use, then the result occurred is also random.Assist sense channel inspection
The detection sense channel result for surveying result and 3 clenbuterol hydrochlorides is mutually obscured, and the corresponding detection project information needs in each channel pass through
Networking decryption can just obtain, can not be from visually seeing testing result.
(2) chromatography to be measured detection card insertion is entered into chart scanner, chart scanner can read the original of bar code and 2 sense channels
Signal is detected, network server is sent collectively to by internet and is remotely decrypted.
(3) network server parses according to the validity of identification code and obtains the corresponding sense channel information of the detection card, from
It is dynamic to analyze the corresponding testing result of each sense channel, it is then that the final detection result after decryption is defeated back to card reading by internet
Instrument.
(4) chart scanner receives the final result returned by network server: Clenbuterol sense channel T line does not develop the color, C line
Colour developing；Ractopamine sense channel T line and C line develop the color；Salbutamol sense channel T line and C line develop the color.Testing result
It is positive for Clenbuterol；Ractopamine, it is negative；Salbutamol, it is negative.Complete display, printing.
As it will be easily appreciated by one skilled in the art that the foregoing is merely illustrative of the preferred embodiments of the present invention, not to
The limitation present invention, any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should all include
Within protection scope of the present invention.
Claims (11)
1. a kind of lateral flow chromatography detection card of encryption, which is characterized in that the detection card includes well (1), sense channel
(2), sense channel (3) and identification code (4) are assisted；The well (1) is located at sense channel (2) and auxiliary sense channel (3)
Side；The identification code (4) records each sense channel (2) and assists the solution confidential information of sense channel (3)；The identification
Code (4) believes the test substance for obtaining sense channel (2) and the corresponding test substance information of auxiliary sense channel (3)
Breath is parsed by network server；The sense channel (2) and auxiliary sense channel (3) are chromatography sense channel；Institute
It states sense channel (2) and is at least 1；Auxiliary sense channel (3) and sense channel (2) determine the method for testing result not
Together；The sense channel (2) and putting in order for sense channel (3) of auxiliary are random.
2. the lateral flow chromatography detection card encrypted as described in claim 1, which is characterized in that when the sense channel (2) is big
When being equal to 2, the sense channel (2) for detecting different testing sample matter respectively simultaneously.
3. the lateral flow chromatography detection card encrypted as described in claim 1, which is characterized in that the sense channel (2) uses
Competition law determines testing result, and the auxiliary sense channel (3) determines testing result using double-antibody method；Or the detection
Channel (2) determines testing result using double-antibody method, and the auxiliary sense channel (3) determines testing result using competition law.
4. the lateral flow chromatography detection card encrypted as described in claim 1, which is characterized in that the sense channel (2) is glue
At least one of body gold immunochromatography channel, latex chromatography channel, magnetic particle chromatography channel and nucleic acid chromatography sense channel；Institute
Stating identification code (4) is bar code, two dimensional code or NFC tags.
5. the lateral flow chromatography detection card encrypted as described in claim 1, which is characterized in that the auxiliary sense channel (3)
Corresponding detection substance is the intrinsic ingredient of determinand, is used for judgement sample authenticity.
6. the lateral flow chromatography detection card encrypted as described in claim 1, which is characterized in that the sense channel (2) is blueness
The gold-marking immunity of mycin chromatographs sense channel；Auxiliary sense channel (3) is that the gold-marking immunity of bovine lactoferrin chromatographs detection
Channel.
7. the lateral flow chromatography detection card encrypted as described in claim 1, which is characterized in that the identification code (4) was recorded
Solution confidential information is the corresponding detection material information in each channel of detection card；Either unique ID of detection card, is obtained by networking
Each Air conduct measurement material information.
8. the lateral flow chromatography detection card encrypted as claimed in claim 3, which is characterized in that the sense channel (2) is pig
The latex of pestivirus chromatographs sense channel, and auxiliary sense channel (3) is that the latex of heavy metal lead chromatographs sense channel；Or
The sense channel (2) is that the latex of heavy metal lead chromatographs sense channel, and auxiliary sense channel (3) is the cream of swine fever virus
Glue-line analyses sense channel.
9. the lateral flow chromatography detection card encrypted as described in claim 1, which is characterized in that the sense channel (2) is Bt
The sense channel of toxoprotein gene；The nature controlling line of auxiliary sense channel (3) is visited with rice phosphoric acid sucrose synthase gene
Needle；The detection line of auxiliary sense channel (3) includes two kinds of situations, is visited with rice phosphoric acid sucrose synthase gene respectively
Needle either blank.
10. the lateral flow chromatography detection card encrypted as claimed in claim 2, which is characterized in that the sense channel (2) has three
Item is Clenbuterol magnetic particle chromatography sense channel, Ractopamine magnetic particle chromatography sense channel and salbutamol magnetic respectively
Particle chromatographs sense channel；The nature controlling line of auxiliary sense channel (3) has secondary antibody；The auxiliary sense channel (3)
Detection line include two kinds of situations, be respectively provided with secondary antibody either blank.
11. the detection method of the lateral flow chromatography detection card such as the described in any item encryptions of claim 1-9, which is characterized in that
The identification code on the detection card that sample has been added dropwise and the detection signal in each channel are read, parses to obtain according to identification code each logical
The corresponding detection material information in road；According to the corresponding detection material information point of the detection signal in each channel and each channel
The corresponding final detection result of each sense channel is analysed, the final detection result is saved, while showing the final detection result.
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| CN109146882A (en) * | 2018-10-26 | 2019-01-04 | 北京纳百生物科技有限公司 | A kind of food safety colloid gold test paper analytical equipment and its application |
| CN109342411A (en) * | 2018-10-31 | 2019-02-15 | 华中科技大学 | With the digital one time Test paper card of short-distance wireless transmission technology and application |
| CN115078713B (en) * | 2022-05-16 | 2023-07-18 | 远大医药（中国）有限公司 | Kit, trusted detection result acquisition method and anti-counterfeiting test paper |
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