CN107440728A - A kind of laboratory mice reaction monitoring instrument - Google Patents
A kind of laboratory mice reaction monitoring instrument Download PDFInfo
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- CN107440728A CN107440728A CN201710591376.8A CN201710591376A CN107440728A CN 107440728 A CN107440728 A CN 107440728A CN 201710591376 A CN201710591376 A CN 201710591376A CN 107440728 A CN107440728 A CN 107440728A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/16—Devices for psychotechnics; Testing reaction times ; Devices for evaluating the psychological state
- A61B5/162—Testing reaction times
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/103—Detecting, measuring or recording devices for testing the shape, pattern, colour, size or movement of the body or parts thereof, for diagnostic purposes
- A61B5/11—Measuring movement of the entire body or parts thereof, e.g. head or hand tremor, mobility of a limb
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/40—Detecting, measuring or recording for evaluating the nervous system
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Abstract
The invention discloses a kind of laboratory mice reaction monitoring instrument, it includes：Mouse accommodating area, grating, grating drive module, photographing module and host computer, mouse accommodating area is made up of a drum, grating is by the alternate closed ring face formed of black and white strip, grating drive module is connected with grating and host computer, host computer sends control signal to drive grating to be rotated using its center of circle as rotating shaft to grating drive module, photographing module is arranged at the top of drum and is connected with host computer, and the mouse images of shooting are sent to host computer；The distance between mouse head length L1, mouse head center and the mouse body center measured under nature L2 and preset ratio P1 is previously stored with host computer, preset ratio P1 is the reference value that normal mouse rotating shutter occurs elongation somatic reaction, host computer is handled each frame mouse images, to judge mouse in the probability of generation height change in time T, and then judge whether mouse Nerve function is normal.
Description
Technical field
The present invention relates to neurology functional study field, in particular to a kind of laboratory mice reaction monitoring instrument.
Background technology
In neurology functional study, behavior motion monitoring is a very important direction, in neurology functional study
In, it is frequently utilized that model animal (such as mouse) carries out related experiment, whether model animal nerve is complete, and whether function is normal, often
Often need artificially to be observed, whether there can be reaction to outer signals intervention by observing it to judge nervous function whether just
Often, so as to further study the pathogenesis or Disease-causing gene of neuropathy.But because model animal can not be retouched with language
Its subjective feeling is stated, and larger difference between Different Individual be present, therefore, the behaviouristics mode data of record and tracking model animal
The always problem of neurology department's research field.At present, although there is some behaviouristics patterns that can record and follow the trail of model animal
The device and system of data, but the problems such as generally existing volume is big, cost is high, accuracy is low and result is unreliable.
The content of the invention
The present invention provides a kind of laboratory mice reaction monitoring instrument, to record and follow the trail of the mode data of pattern mouse,
And then judge whether the nervous function of mouse is normal.
To reach above-mentioned purpose, the invention provides a kind of laboratory mice reaction monitoring instrument, it includes：Mouse houses
Area, grating, grating drive module, photographing module and host computer, wherein：
The mouse accommodating area is made up of a drum, and the drum has bottom surface and side wall and side wall is transparent；
The grating be by the alternate closed ring face formed of black and white strip, its be arranged on the inner side of the drum and with
The concyclic heart of drum；
The grating drive module is connected with the grating and the host computer, and the host computer drives to the grating
Module sends control signal to drive the grating to be rotated using its center of circle as rotating shaft；
The photographing module is arranged at the top of the drum and is connected with the host computer, and the mouse images of shooting are sent out
Deliver to the host computer；
The mouse head length L1 measured under nature, mouse head center are previously stored with the host computer
The distance between mouse body center L2 and preset ratio P1, preset ratio P1 are that normal mouse is stretched to rotating shutter
Grow up the reference value of reaction, the host computer carries out following handle to each frame mouse images：
(1) rim detection and binary conversion treatment are carried out to mouse images, to obtain corresponding gray level image A,
(2) gray level image A centre of form a1 is calculated；
(3) institute positioned at gray level image A edges is calculated the distance between a little with centre of form a1, apart from side corresponding to maximum
Edge point is mouse nose point b1；
(4) using b1 as the center of circle, a border circular areas C is drawn by radius of mouse head length L1；
(5) centre of form c1 of gray level image A and border circular areas C intersection area is calculated, centre of form c1 is the head of mouse；
(6) the distance between centre of form c1 and the centre of form a1 L3 are calculated；
(7) judge whether L3 is more than 1.3 × L2, if it has, then the frame mouse images are labeled as into " 1 ", if it has not, then will
The frame mouse images are labeled as " 0 "；
(8) host computer according to a length of T during above-mentioned steps (1)~(7) continuous processing mouse images, and calculate during this period
The ratio P of all mouse images is accounted for labeled as the mouse images of " 1 ", if P >=P1, judges that the mouse Nerve function is normal, if P
＜ P1, then judge that the mouse Nerve function is abnormal.
In one embodiment of this invention, a diameter of 15cm of the drum, the closed ring face of the optical grating constitution
A diameter of 12cm.
In one embodiment of this invention, the grating drive module includes a stepper motor and motor driving mould
Block.
In one embodiment of this invention, the motor drive module includes single-chip microcomputer STM32F103 and motor driving mould
Block L298N.
In one embodiment of this invention, the material of the side wall of the drum is acrylic.
In one embodiment of this invention, the width of black and white striped is 2cm in the grating.
In one embodiment of this invention, the frequency that the grating rotates is 0.5Hz.
In one embodiment of this invention, the photographing module uses free drive automatic focusing camera head, picture-taken frequency
For 24 frames/second.
In one embodiment of this invention, laboratory mice reaction monitoring instrument also includes a wireless transport module, the nothing
Line transport module is connected with the host computer.
In one embodiment of this invention, T is two minutes.
Laboratory mice reaction monitoring instrument small volume provided by the invention, low cost, analysis result are precisely reliable and readable
Property it is strong, be available for Neuscience researcher independent operation, filled up in the market animal nerve mode data record and tracking
The technological gap in field, there is important scientific research value and wide commercial application prospect.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the required accompanying drawing used in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the schematic diagram of the laboratory mice reaction monitoring instrument of one embodiment of the invention.
Description of reference numerals：1- mouse accommodating area；11- bottom surfaces；12- side walls；2- gratings；3- grating drive modules；4- takes the photograph
As module；5- host computers.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not paid
Embodiment, belong to the scope of protection of the invention.
The principle of the present invention is the neurology reaction based on mouse, i.e., the good mouse of vision, cinesthesia function is to motion
Object there is vision to follow and extend body forward and visit with the phenomenon of tracing movement object, the moving object in the present invention is
Grating, the body image of the mouse after grating is seen by collection and analyzes the change of mouse height, judgment experiment mouse Nerve work(
Whether can be damaged.If mouse sees the height change that greater probability is produced after the grating of motion, judge that mouse Nerve function is good
Good, if mouse sees that the probability that height change is produced after the grating of motion is little, it is caused body unintentionally to be judged as mouse
Long change, i.e. height change is unrelated with the grating moved, then the nervous function of mouse should receive damage.
Fig. 1 is the schematic diagram of the laboratory mice reaction monitoring instrument of one embodiment of the invention, as shown in figure 1, of the invention
The laboratory mice reaction monitoring instrument of offer includes：Mouse accommodating area 1, grating 2, grating drive module 3, photographing module 4 and on
Position machine 5, wherein：
Mouse accommodating area 1 is made up of a drum, and drum has bottom surface 11 and side wall 12 and side wall 12 is transparent；
The diameter of drum in the present invention can be decided according to the actual requirements, and generally require straight according to the selection of the size of mouse
The suitable drum in footpath, activity freely and mouse can be facilitated to turn round be defined in drum by mouse.For example, root in the present embodiment
According to a diameter of 15cm of the drum of mouse size selection.The side wall of drum can for example select the transparency, chemical stability and resistance to
The preferable acrylics of Hou Xingjun.In addition, the background color of mouse accommodating area 1 and the hair color of mouse are preferred with easy differentiation, in order to rear
Continuous image procossing is smoothed out, in addition, mouse accommodating area 1 should not have multi-pollutant to be deposited such as stool in mice, mouse hair
If that should remove in time.
Grating 2 is that it is arranged on the inner side of drum and is total to drum by the alternate closed ring face formed of black and white strip
The center of circle；
Grating 2 is arranged on the inner side of drum, and its diameter is slightly less than the diameter of drum, the closing of optical grating constitution in the present embodiment
A diameter of 12cm of ring surface, the width of black and white striped is 2cm in grating 2.
Grating drive module 3 is connected with grating 2 and host computer 5, and host computer 5 sends control letter to grating drive module 3
Number to drive grating 2 to be rotated using its center of circle as rotating shaft；
Mouse accommodating area 1, grating 2 in Fig. 1 etc. are presented with section view diagram form, and those skilled in the art can according to Fig. 1
In the form of it is envisaged that its is complete, repeated no more in this.
Grating drive module 3 can include a stepper motor and a motor drive module (not shown), stepping electricity
Machine is connected to drive grating to rotate with grating 2, and motor drive module is connected with host computer to be believed with receiving the control of host computer transmission
Number, motor drive module can for example use single-chip microcomputer STM32F103 and motor drive module L298N to realize, can control step
Stepper motor smooth starting and change rotating speed, rotating speed controlled range is 0.1Hz-5Hz, and the rotating speed of stepper motor is identical with grating 2,
In the present embodiment, the frequency that grating 2 rotates is 0.5Hz.
Photographing module 4 is arranged at the top of drum and is connected with host computer 5, and the mouse images of shooting are sent to host computer
5；
Photographing module 4 in the present embodiment uses free drive automatic focusing camera head, and picture-taken frequency is 24 frames/second.This
Invention can also select other to absorb the equipment of image as needed and carry out IMAQ, and picture-taken frequency also can be according to reality
Border needs and changed.
The running environment of host computer such as following table in the present embodiment：
Be previously stored with host computer 5 the mouse head length L1 measured under nature, mouse head center with
The distance between mouse body center L2 and preset ratio P1, preset ratio P1 are that normal mouse extends to rotating shutter
The reference value of somatic reaction, host computer 5 carry out following handle to each frame mouse images：
(1) rim detection and binary conversion treatment are carried out to mouse images, to obtain corresponding gray level image A,
The step makes a distinction mouse and the background color of mouse accommodating area 1, obtains the mouse images of complete display, edge inspection
Survey is to reject the jamming patterns such as stool in mice.Further, since the tail of mouse is unrelated with follow-up image procossing, the step
In the tail of mouse can be filtered out by binary conversion treatment process.
(2) gray level image A centre of form a1 is calculated；
(3) institute positioned at gray level image A edges is calculated the distance between a little with centre of form a1, apart from side corresponding to maximum
Edge point is mouse nose point b1；
(4) using b1 as the center of circle, a border circular areas C is drawn by radius of mouse head length L1；
(5) centre of form c1 of gray level image A and border circular areas C intersection area is calculated, centre of form c1 is the head of mouse；
(6) the distance between centre of form c1 and the centre of form a1 L3 are calculated；
(7) judge whether L3 is more than 1.3 × L2, if it has, then the frame mouse images are labeled as into " 1 ", if it has not, then will
The frame mouse images are labeled as " 0 "；
(8) host computer according to a length of T during above-mentioned steps (1)~(7) continuous processing mouse images, and calculate during this period
The ratio P of all mouse images is accounted for labeled as the mouse images of " 1 ", if P >=P1, judges that the mouse Nerve function is normal, if P
＜ P1, then judge that the mouse Nerve function is abnormal.
T in the present embodiment is two minutes, that is, in gathering 48 altogether according to 24 frames of picture-taken frequency/second in two minutes
Two field picture, the number of the mouse images of " 1 " is labeled as during calculating this, the ratio with 48 is P.It is default in the present embodiment
Ratio P1 can be set to 70% (or other numerical value between 70% to 100%), i.e. if accounted for labeled as the mouse images of " 1 "
The ratio of all mouse images is more than 70%, then judges that the nervous function of mouse is good.
In other embodiments of the invention, laboratory mice reaction monitoring instrument can further include one and be wirelessly transferred
Module (not shown), wireless transport module are connected with host computer, and therefore, photographing module 4 and grating drive module 3 pass through
Wireless transport module is realized to be transmitted the data between host computer, signal, and therefore, host computer can be arranged on distance and test with small
At a certain distance from mouse reaction monitoring instrument other parts, to realize remote control.
Laboratory mice reaction monitoring instrument small volume provided by the invention, low cost, analysis result are precisely reliable and readable
Property it is strong, be available for Neuscience researcher independent operation, filled up in the market animal nerve mode data record and tracking
The technological gap in field, there is important scientific research value and wide commercial application prospect.
One of ordinary skill in the art will appreciate that：Accompanying drawing is the schematic diagram of one embodiment, module in accompanying drawing or
Flow is not necessarily implemented necessary to the present invention.
One of ordinary skill in the art will appreciate that：The module in device in embodiment can describe to divide according to embodiment
It is distributed in the device of embodiment, respective change can also be carried out and be disposed other than in one or more devices of the present embodiment.On
The module for stating embodiment can be merged into a module, can also be further split into multiple submodule.
Finally it should be noted that：The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
The present invention is described in detail with reference to the foregoing embodiments, it will be understood by those within the art that：It still may be used
To be modified to the technical scheme described in previous embodiment, or equivalent substitution is carried out to which part technical characteristic；And
These modifications are replaced, and the essence of appropriate technical solution is departed from the spirit and model of technical scheme of the embodiment of the present invention
Enclose.
Claims (10)
- A kind of 1. laboratory mice reaction monitoring instrument, it is characterised in that including：Mouse accommodating area, grating, grating drive module, Photographing module and host computer, wherein：The mouse accommodating area is made up of a drum, and the drum has bottom surface and side wall and side wall is transparent；The grating be by the alternate closed ring face formed of black and white strip, its be arranged on the inner side of the drum and with it is described The concyclic heart of drum；The grating drive module is connected with the grating and the host computer, and the host computer is to the grating drive module Control signal is sent to drive the grating to be rotated using its center of circle as rotating shaft；The photographing module is arranged at the top of the drum and is connected with the host computer, by the mouse images of shooting send to The host computer；Be previously stored with the host computer mouse head length L1 measured under nature, mouse head center with it is small The distance between mouse body centre L2 and preset ratio P1, preset ratio P1 are that normal mouse rotating shutter occurs elongation body The reference value of precursor reactant, the host computer carry out following handle to each frame mouse images：(1) rim detection and binary conversion treatment are carried out to mouse images, to obtain corresponding gray level image A,(2) gray level image A centre of form a1 is calculated；(3) institute positioned at gray level image A edges is calculated the distance between a little with centre of form a1, apart from marginal point corresponding to maximum As mouse nose point b1；(4) using b1 as the center of circle, a border circular areas C is drawn by radius of mouse head length L1；(5) centre of form c1 of gray level image A and border circular areas C intersection area is calculated, centre of form c1 is the head of mouse；(6) the distance between centre of form c1 and the centre of form a1 L3 are calculated；(7) judge whether L3 is more than 1.3 × L2, if it has, then the frame mouse images are labeled as into " 1 ", if it has not, then by the frame Mouse images are labeled as " 0 "；(8) host computer according to a length of T during above-mentioned steps (1)~(7) continuous processing mouse images, and calculate mark during this period The ratio P of all mouse images is accounted for for the mouse images of " 1 ", if P >=P1, judges that the mouse Nerve function is normal, if P ＜ P1, then judge that the mouse Nerve function is abnormal.
- 2. laboratory mice reaction monitoring instrument according to claim 1, it is characterised in that the drum it is a diameter of 15cm, a diameter of 12cm in the closed ring face of the optical grating constitution.
- 3. laboratory mice reaction monitoring instrument according to claim 1, it is characterised in that the grating drive module includes One stepper motor and a motor drive module.
- 4. laboratory mice reaction monitoring instrument according to claim 3, it is characterised in that the motor drive module includes Single-chip microcomputer STM32F103 and motor drive module L298N.
- 5. laboratory mice reaction monitoring instrument according to claim 1, it is characterised in that the material of the side wall of the drum For acrylic.
- 6. laboratory mice reaction monitoring instrument according to claim 1, it is characterised in that black and white in the grating The width of striped is 2cm.
- 7. laboratory mice reaction monitoring instrument according to claim 1, it is characterised in that the frequency that the grating rotates is 0.5Hz。
- 8. laboratory mice reaction monitoring instrument according to claim 1, it is characterised in that the photographing module uses free drive Automatic focusing camera head, picture-taken frequency are 24 frames/second.
- 9. laboratory mice reaction monitoring instrument according to claim 1, it is characterised in that be also wirelessly transferred mould including one Block, the wireless transport module are connected with the host computer.
- 10. the laboratory mice reaction monitoring instrument according to any one of claim 1-9, it is characterised in that T is two points Clock.
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| CN201710591376.8A CN107440728A (en) | 2017-07-19 | 2017-07-19 | A kind of laboratory mice reaction monitoring instrument |
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Cited By (1)
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| CN111296307A (en) * | 2020-02-12 | 2020-06-19 | 南开大学 | Small animal behavior track acquisition device |
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