CN107436359A - Ferritin diagnostic kit - Google Patents
Ferritin diagnostic kit Download PDFInfo
- Publication number
- CN107436359A CN107436359A CN201710613714.3A CN201710613714A CN107436359A CN 107436359 A CN107436359 A CN 107436359A CN 201710613714 A CN201710613714 A CN 201710613714A CN 107436359 A CN107436359 A CN 107436359A
- Authority
- CN
- China
- Prior art keywords
- ferritin
- monoclonal antibody
- diagnostic kit
- line
- pad
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/96—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
Abstract
The present invention relates to medical treatment detection device technical field, and in particular to a kind of ferritin diagnostic kit；A kind of ferritin diagnostic kit of the present invention, include the Test paper and colorimetric card of ferritin；The Test paper includes collaurum plastic bottom board, is pasted with sample pad, the colloidal gold pad for the colloid gold particle that the Anti-ferritin monoclonal antibody of coating pairing marks, nitrocellulose filter, detection line T, nature controlling line C successively on the collaurum plastic bottom board；The nitrocellulose filter overlaps with colloidal gold pad, and the other end and the sample pad of the colloidal gold pad overlap；The nature controlling line is coated with sheep anti-mouse igg antibody, and the detection line is coated with Anti-ferritin monoclonal antibody；The colorimetric card is provided with 10ng/ml, 20ng/ml, 50ng/ml ratio colo(u)r streak；Ferritin Levels of the kit of the present invention suitable for external half-quantitative detection human serum, blood plasma and whole blood, it is functional, it is low available for clinical detection, testing cost；Kit is easy to operate.
Description
Technical field
The present invention relates to medical treatment detection device technical field, and in particular to a kind of ferritin diagnostic kit.
Background technology
Serum ferritin abbreviation ferritin (serum ferritsn, SF), a kind of compound protein of iron content, ferritin
It is present in each tissue and cell in vivo, especially in spleen, mucous membrane of small intestine, in liver and red marrow.Ferritin can preserve iron
In hollow globular protein, prevent that endocellular liberation iron is excessive and produce illeffects while can be as the depots of iron
Synthesized for hemoglobin：Acidic Ferritin then plays iron transfer, its alkaline ferritin and holds and has pass.Epidemiology survey
It was found that the content of ferritin is that 33.9 ± 16.3ng/ml. women is 32.7 ± 16.4ng/m1 in normal male, but women exists
The gestational period can be due to reducing during acute anemia, and acute and chronic liver damage even hepatocarcinoma patient then has notable rise.Therefore can
Rapidly, sensitive, the accurate SFF values for detecting and tracking in serum, blood plasma and whole blood have higher clinical value.
Determination of Serum Ferritin method has a variety of at present, such as radio-immunity double antibody method, ELISA and chemiluminescence
Method etc..But the characteristics of time-consuming, effort and consumption money be present in present detection method, or even the defects of testing result is not accurate enough.
The content of the invention
Time-consuming, effort be present for the existing Determination of Serum Ferritin method of solution and consume the technical problem of money in the present invention, there is provided
A kind of ferritin diagnostic kit.
In order to solve the above technical problems, the technical solution adopted in the present invention is：A kind of ferritin diagnostic kit, including
The Test paper and colorimetric card of ferritin；The Test paper includes collaurum plastic bottom board, in the collaurum plastic bottom board
On be pasted with sample pad, the colloidal gold pad of colloid gold particle of Anti-ferritin monoclonal antibody mark of coating pairing, nitre successively
Acid cellulose film；Detection line T, nature controlling line C are set on the nitrocellulose filter；The nitrocellulose filter and colloidal gold pad
One end overlaps, and the other end and the sample pad of the colloidal gold pad overlap；The nature controlling line is coated with sheep anti-mouse igg antibody, described
Detection line is coated with Anti-ferritin monoclonal antibody；The colorimetric card is provided with 10ng/ml, 20ng/ml, 50ng/ml colorimetric
Line, it is described than colo(u)r streak by the different red segment of the depth.
Above-mentioned ferritin diagnostic kit detection method is as follows：
1）Detection kit and sample are placed on and balanced 2 hours at room temperature.
2）Extract 10 μ l serum or plasma sample, when sample is whole blood if draw 20 μ l, be added to 10 times of sample
In product dilution, fully mix；
3）Then take 100 μ l dilute samples to be put into clean centrifuge tube with pipettor, test card is put into centrifuge tube 2-3 points
Zhong Hou, take out test paper and keep flat, read result in 15 minutes, be compared with colorimetric card.
Compared with prior art the invention has the advantages that.
Ferritin Levels of the kit of the present invention suitable for external half-quantitative detection human serum, blood plasma and whole blood, property
Can be good, available for clinical detection.Kit of the present invention does not need any special instruments and equipment, and testing cost is low；Kit is grasped
Make easy, it is not necessary to which professional operates.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1
A kind of ferritin diagnostic kit, include the Test paper and colorimetric card of ferritin；The Test paper includes collaurum
Plastic bottom board, it is pasted with sample pad, the Anti-ferritin monoclonal antibody of coating pairing successively on the collaurum plastic bottom board
The colloidal gold pad of the colloid gold particle of mark, nitrocellulose filter；Detection line T, nature controlling line are set on the nitrocellulose filter
C；One end of the nitrocellulose filter and colloidal gold pad overlaps, and the other end and the sample pad of the colloidal gold pad overlap；It is described
Nature controlling line is coated with sheep anti-mouse igg antibody, and the detection line is coated with Anti-ferritin monoclonal antibody；The colorimetric card is provided with
10ng/ml, 20ng/ml, 50ng/ml ratio colo(u)r streak, it is described than colo(u)r streak by the different red segment of the depth.
The Anti-ferritin monoclonal antibody is made up of the first monoclonal antibody and second monoclonal antibody, and described first is single
Clonal antibody by deposit number be CGMCC NO.10595 hybridoma cell strain secrete to obtain, the second monoclonal antibody by
Deposit number is that CGMCC NO.10594 hybridoma cell strain is secreted to obtain.
Described detection line T 12mm apart from the edge of nitrocellulose filter.
Described nature controlling line C 7mm apart from the edge of nitrocellulose filter.
Described detection line T and nature controlling line C distances are 5mm.
Embodiment 2
A kind of ferritin diagnostic kit, include the Test paper and colorimetric card of ferritin；The Test paper includes collaurum
Plastic bottom board, it is pasted with sample pad, the Anti-ferritin monoclonal antibody of coating pairing successively on the collaurum plastic bottom board
The colloidal gold pad of the colloid gold particle of mark, nitrocellulose filter；Detection line T, nature controlling line are set on the nitrocellulose filter
C；One end of the nitrocellulose filter and colloidal gold pad overlaps, and the other end and the sample pad of the colloidal gold pad overlap；It is described
Nature controlling line is coated with sheep anti-mouse igg antibody, and the detection line is coated with Anti-ferritin monoclonal antibody；The colorimetric card is provided with
10ng/ml, 20ng/ml, 50ng/ml ratio colo(u)r streak, it is described than colo(u)r streak by the different red segment of the depth.
The Anti-ferritin monoclonal antibody is made up of the first monoclonal antibody and second monoclonal antibody, and described first is single
Clonal antibody by deposit number be CGMCC NO.10595 hybridoma cell strain secrete to obtain, the second monoclonal antibody by
Deposit number is that CGMCC NO.10594 hybridoma cell strain is secreted to obtain.
Described detection line T 15mm apart from the edge of nitrocellulose filter.
Described nature controlling line C 6mm apart from the edge of nitrocellulose filter.
Described detection line T and nature controlling line C distances are 9mm.
The explanation of assay
1. one nature controlling line of appearance（C）Or response line（T）Color intensity be less than or equal to colorimetric card 10ng/ml lines, explanation
The Ferritin Levels of sample are less than 10ng/ml.T line colors compare between 10-50ng/ml and colorimetric card compares sxemiquantitative judgement
As a result.T line colors are better than colorimetric card 50ng/ml red lines, illustrate that sample Ferritin Levels are higher than 50ng/ml.
2. detection sample Ferritin Levels are less than or equal to 20ng/ml, the possible iron deficiency of testee is prompted, more than 20ng/
Ferro element may be had no lack of in ml prompting testee's bodies.
3. occur without nature controlling line（C）Only there is a response line red line in red line, and experimental result is null result, Ying Chong
Examination.
Contrasted using detection kit of the present invention and 300 parts of immunofluorescence results, testing result finds sharing 296 parts of samples
Product are consistent, and uniformity is more than 0.98.Show that the kit prepared is functional, can be used for clinical detection.
The present invention can be summarized with others without prejudice to the concrete form of the spirit or essential characteristics of the present invention.Therefore, nothing
By from the point of view of which point, the embodiment above of the invention can only all be considered the description of the invention and can not limit invention,
Claims indicate the scope of the present invention, and above-mentioned explanation does not point out the scope of the present invention, therefore, with the present invention
The suitable implication and scope of claims in any change, be all considered as being included within the scope of the claims.
Claims (5)
1. a kind of ferritin diagnostic kit, it is characterised in that Test paper and colorimetric card including ferritin；The detection examination
Paper includes collaurum plastic bottom board, is pasted with sample pad, the iron-resistant egg of coating pairing successively on the collaurum plastic bottom board
The colloidal gold pad of the colloid gold particle of white labeling of monoclonal antibody, nitrocellulose filter；Inspection is set on the nitrocellulose filter
Survey line T, nature controlling line C；One end of the nitrocellulose filter and colloidal gold pad overlaps, the other end and sample of the colloidal gold pad
Pad overlap joint；The nature controlling line is coated with sheep anti-mouse igg antibody, and the detection line is coated with Anti-ferritin monoclonal antibody；It is described
Colorimetric card is provided with 10ng/ml, 20ng/ml, 50ng/ml ratio colo(u)r streak, it is described than colo(u)r streak by the different red segment of the depth.
2. ferritin diagnostic kit according to claim 1, it is characterised in that the Anti-ferritin monoclonal antibody by
First monoclonal antibody and second monoclonal antibody composition, first monoclonal antibody is CGMCC by deposit number
NO.10595 hybridoma cell strain is secreted to obtain, and the second monoclonal antibody is CGMCC NO.10594's by deposit number
Hybridoma cell strain is secreted to obtain.
3. ferritin diagnostic kit according to claim 1, it is characterised in that described detection line T is fine apart from nitric acid
Tie up the edge 3-15mm of plain film.
4. ferritin diagnostic kit according to claim 1, it is characterised in that described nature controlling line C is fine apart from nitric acid
Tie up the edge 5-8mm of plain film.
5. ferritin diagnostic kit according to claim 1, it is characterised in that described detection line T and nature controlling line C
Distance is 4-9mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710613714.3A CN107436359A (en) | 2017-07-25 | 2017-07-25 | Ferritin diagnostic kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710613714.3A CN107436359A (en) | 2017-07-25 | 2017-07-25 | Ferritin diagnostic kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107436359A true CN107436359A (en) | 2017-12-05 |
Family
ID=60461129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710613714.3A Pending CN107436359A (en) | 2017-07-25 | 2017-07-25 | Ferritin diagnostic kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107436359A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020016571A1 (en) * | 2018-07-16 | 2020-01-23 | Donnelly George Arthur | Ferritin analysis via lateral flow immunoassay |
| CN113777322A (en) * | 2021-09-06 | 2021-12-10 | 珠海科域生物工程股份有限公司 | Fecal calprotectin detection kit and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202614770U (en) * | 2012-05-22 | 2012-12-19 | 北京易斯威特生物医学科技有限公司 | Ferritin quantitative detection system |
| CN202631544U (en) * | 2012-04-06 | 2012-12-26 | 天津中新科炬生物制药有限公司 | External detection reagent box for quick semi-quantitative detection of ferritin concentration |
| CN105131111A (en) * | 2015-07-30 | 2015-12-09 | 中国检验检疫科学研究院 | Human lactoferrin monoclonal antibody pair |
| CN205608007U (en) * | 2016-03-31 | 2016-09-28 | 珠海华澳生物科技有限公司 | Ferritin detect reagent box |
-
2017
- 2017-07-25 CN CN201710613714.3A patent/CN107436359A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202631544U (en) * | 2012-04-06 | 2012-12-26 | 天津中新科炬生物制药有限公司 | External detection reagent box for quick semi-quantitative detection of ferritin concentration |
| CN202614770U (en) * | 2012-05-22 | 2012-12-19 | 北京易斯威特生物医学科技有限公司 | Ferritin quantitative detection system |
| CN105131111A (en) * | 2015-07-30 | 2015-12-09 | 中国检验检疫科学研究院 | Human lactoferrin monoclonal antibody pair |
| CN205608007U (en) * | 2016-03-31 | 2016-09-28 | 珠海华澳生物科技有限公司 | Ferritin detect reagent box |
Non-Patent Citations (1)
| Title |
|---|
| 张改平: "《免疫层析试纸快速检测技术》", 31 August 2015 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020016571A1 (en) * | 2018-07-16 | 2020-01-23 | Donnelly George Arthur | Ferritin analysis via lateral flow immunoassay |
| GB2578944A (en) * | 2018-07-16 | 2020-06-03 | Arthur Donnelly George | Analyser |
| CN113777322A (en) * | 2021-09-06 | 2021-12-10 | 珠海科域生物工程股份有限公司 | Fecal calprotectin detection kit and preparation method thereof |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171205 |
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| RJ01 | Rejection of invention patent application after publication |