CN107418895A - A kind of biotechnology cell culture apparatus - Google Patents
A kind of biotechnology cell culture apparatus Download PDFInfo
- Publication number
- CN107418895A CN107418895A CN201710861615.7A CN201710861615A CN107418895A CN 107418895 A CN107418895 A CN 107418895A CN 201710861615 A CN201710861615 A CN 201710861615A CN 107418895 A CN107418895 A CN 107418895A
- Authority
- CN
- China
- Prior art keywords
- cell culture
- fixedly connected
- culture apparatus
- fixed plate
- biotechnology
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Thermal Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of biotechnology cell culture apparatus, including casing, the side of the casing inner wall is fixedly connected with supporting plate, and the top movable of supporting plate is connected with shaking table, both sides at the top of the shaking table have been fixedly connected with fixing device, the fixing device includes fixed plate, the bottom of the fixed plate at the top of shaking table with being fixedly connected, and the top movable of fixed plate is connected with conical flask, both sides at the top of the fixed plate have been respectively fixedly connected with support bar, and the surface of conical flask is connected with fixture block, the side of the fixture block is fixedly connected with the surface of support bar, the present invention relates to technical field of cell culture.The biotechnology cell culture apparatus, change because culture apparatus fixes unstable, the problem of loosening bottle stopper, prevent the pollution of miscellaneous bacteria in cell cultivation process, the probability of cell culture failure is greatly reduced, considerably increases its feature, whole operation process is simple and convenient.
Description
Technical field
The invention belongs to technical field of cell culture, more particularly to a kind of biotechnology cell culture apparatus.
Background technology
Rise with molecular biology and the infiltration to each side, each subdiscipline of bioscience also experiencing the ups and downs
The change of replacement.From the point of view of current state of development, molecular biology will keep taking the lead the status of subdiscipline, primary study
Field be：The research of the 26S Proteasome Structure and Function of large biological molecule；The research of eukaryotic gene and gene expression regulation；Molecule god
Research through biology；The research of Medical Molecular Biology；The research of molecular biology of plants；Research of molecular evolution, etc..
As can be seen here, molecular biology has driven the development in an all-round way of whole bioscience, and this is one of contemporary bioscience significantly special
Point and development trend.
Modern biotechnology the reach of science, it is to intersect between the science such as bioscience and mathematics, physics, chemistry, ooze
Result that is saturating and mutually promoting.Other related sciences promoted bioscience biological phenomena and this Quality Research are deepened continuously and
Expand, the development of bioscience also proposes many new research topics for other related sciences, opens many new researchs
Field.It can be seen that the two-way penetration and synthesis of bioscience and the height about science, also have become the one of contemporary bioscience
Individual distinguishing feature and development trend.
The new development of modern biotechnology science, it is many to be obtained under conditions of using advanced technology and means, these
New technology has：DNA recombinant techniques, DNA synthetic technologys, rapid DNA sequencing technology, the artificial synthesized technology of protein, albumen
Matter sequencing technology, hybridization, restriction enzyme fragment length diversity techniques, Antisense RNA Technique, gather
Synthase chain reaction amplification technique, monoclonal antibody technique, pulse electrophoresis technology, magnetic force resonance technology, scanning-tunnelling and atomic force
Microtechnic, Synchrotron Radiation Technology, electronic computer technology, etc..It can be seen that the innovation of investigative technique and means is contemporary biology
Another distinguishing feature and development trend of science.
In the last few years, ecological research especially caused the concern of people.Due to the mankind the whole world life condition increasingly
Deteriorate, ecology just gets up with integration of disciplines such as mathematics, geosciences, studies the interaction of each ring layer of the earth and its causes
Whole world change.With the development of molecular biology, biologist also begins to study the pass of biology and environment on a molecular scale
System.The development of this macroscopical and microcosmic both sides and combination are the contemporary ecological key characters developed.Ecology is
As the criterion and scientific basis of the following global economy sustainable development of guidance.It can be seen that the great attention to ecological study, and
A distinguishing feature and development trend for contemporary bioscience.
Distinguished biologists Wilson's early in the 1920s just propose ana, and " all living things key issue must
Must be looked in cell ", also there is very deep intension so far.Weismann and Morgan all attempted the base in cell research once
Heredity, development and unified theory of evolving are established on plinth, although the approach specifically solved was not found at that time, on cell
Importance of the knowledge in bioscience is obvious.Cell is all life bascule and the base unit of function,
Cell biology is to study the science of cell activities basic law, the structure of cell.Cell metabolism, cytogenetics, cell
Propagation and differentiation, the transmission of cellular informatics and the communication of cell etc. be cell biology main research.It is although thin from now on
The content of born of the same parents' biological study is comprehensive, but it is probably two basic points to sum up：
First, how gene controls the important vital movement of cell with gene outcome, such as growth, propagation, differentiation and aging,
This to be related to one it is brand-new the problem of, how intraor extracellular signal transmits；Second, the protein molecule of gene outcome one and other
How biomolecule builds and is assembled into the structure of cell, and exercises the orderly vital movement of cell.
For the hot fields of future biological research from now at the beginning of 21 century, the research of molecular biology will drive biological section
Develop by leaps and bounds comprehensively, numerous subdisciplines of bioscience, by the great synthesis of the realization theory on higher level.Promote to give birth to
Thing science has to the hot research field that high level is broken through：The 26S Proteasome Structure and Function of large biological molecule, gene and cell, heredity, development
With the unification of evolution, brain science, behavior science, ecology etc..
Cell clone technology is also in cell culture, and the regular noun in biology is cell culture technology.No matter for
For whole biotechnology, or the biological cloning technology of one of them, cell culture is all an essential mistake
Journey, the extensive clone of cell culture inherently cell.Cell culture technology can by a cell by mass propgation into
For many cells that are simple unicellular or seldom breaking up, this is the essential link of clone technology, and cell culture is in itself
It is exactly the clone of cell.Substantial amounts of cell or its metabolite are obtained by cell culture.Because biological product is all from cell
Get, it is possible to which it is most crucial, most basic technology in biotechnology to say cell culture technology.
Modern biotechnology is generally thought to comprise technique for gene engineering, cell engineering, enzyme engineering technology and fermentation work
Journey technology, and the development of these technologies nearly all has substantial connection, the particularly development in field of medicaments, cell with cell culture
Culture has more special effect and value.For example genetically engineered drug or vaccine are much by thin during researching and producing
Born of the same parents cultivate to realize.Recombinant hepatitis b vaccine is much to be used as carrier using Chinese hamster ovary celI；It is even more from not cell in cell engineering
Culture, authentic monoclonal antibody, is entirely to be realized by cell culture, it is the genetic engineering developed rapidly now both to have made
Antibody also be unable to do without cell culture.Valued gene therapy, treated autologous cell also will pass through cell cultivation process just again
It can realize, Fermentation Engineering and enzyme engineering some are also closely related with cell culture.In a word, cell culture is produced in whole biotechnology
Very crucial central role is served in the development of industry.
Cell culture is usually that primary cell is sealed in conical flask, there is provided suitable temperature, shakes and carries out by shaking table
Fast culture, but due to cell culture during can produce gas, then the concussion by shaking table, due to culture apparatus fix not
It is stable, bottle stopper is loosened, is easy to bring miscellaneous bacteria in air in nutrient solution into, causes cell culture to fail.
The content of the invention
In view of the shortcomings of the prior art, the invention provides a kind of biotechnology cell culture apparatus, solve due to
Culture apparatus fixes unstable, the problem of loosening bottle stopper.
To realize object above, the present invention is achieved by the following technical programs：A kind of biotechnology cell culture
Device, including casing, the side of the casing inner wall is fixedly connected with supporting plate, and the top movable of supporting plate is connected with and shaken
Bed, the both sides at the top of the shaking table have been fixedly connected with fixing device.
In order to further improve the performance of biotechnology cell culture apparatus, the fixing device includes fixing
Plate, with being fixedly connected at the top of shaking table, the both sides at the top of the fixed plate have been respectively fixedly connected with branch for the bottom of the fixed plate
Strut.
In order to further improve the performance of biotechnology cell culture apparatus, the top of the fixed plate
Portion is connected with conical flask, and the surface of conical flask is connected with fixture block, the side of the fixture block and the table of support bar
Face is fixedly connected.
In order to further improve the performance of biotechnology cell culture apparatus, the top of the casing inner wall is fixed
It is connected with LED illumination lamp.
In order to further improve the performance of biotechnology cell culture apparatus, the side of the casing inner wall is fixed
It is connected with temperature controller.
In order to further improve the performance of biotechnology cell culture apparatus, the side of the casing is flexibly connected
There is safety door.
In order to further improve the performance of biotechnology cell culture apparatus, the side of the safety door, which is fixed, to be connected
It is connected to handle.
In order to further improve the performance of biotechnology cell culture apparatus, the both sides of the bottom of shell are solid
Surely it is connected with Convenient table.
Beneficial effect
The invention provides a kind of biotechnology cell culture apparatus.Possesses following beneficial effect：
（1）The biotechnology cell culture apparatus, by the bottom of the fixed plate with being fixedly connected at the top of shaking table, and
The top movable of fixed plate is connected with conical flask, and the both sides at the top of the fixed plate have been respectively fixedly connected with support bar, and bore
The surface of shape bottle is connected with fixture block, and the side of the fixture block is fixedly connected with the surface of support bar, changed due to culture apparatus
Fixation is unstable, the problem of loosening bottle stopper, it is therefore prevented that the pollution of miscellaneous bacteria in cell cultivation process, greatly reduce cell culture
The probability of failure.
（2）The biotechnology cell culture apparatus, by being fixedly connected with illuminating lamp, and machine at the top of casing inner wall
The side of shell inwall is fixedly connected with temperature controller, and the side of casing is connected with safety door, and the side of safety door
Handle is fixedly connected with, changes the single structure of in the market cell culture apparatus, considerably increases its feature, whole operation
Process is simple and convenient.
Brief description of the drawings
Fig. 1 is schematic structural view of the invention.
Fig. 2 is the structural representation of fixing device of the present invention.
In figure：1 casing, 2 supporting plates, 3 shaking tables, 4 fixing devices, 41 fixed plates, 42 conical flasks, 43 support bars, 44 fixture blocks,
5 illuminating lamps, 6 temperature controllers, 7 safety doors, 8 handles, 9 Convenient tables.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
Fig. 1-2 is referred to, the present invention provides a kind of technical scheme：A kind of biotechnology cell culture apparatus, including machine
Shell 1, the side of the inwall of casing 1 is fixedly connected with supporting plate 2, and the top movable of supporting plate 2 is connected with shaking table 3, and shaking table 3 pushes up
The both sides in portion have been fixedly connected with fixing device 4, and fixing device 4 includes fixed plate 41, the bottom of fixed plate 41 and the top of shaking table 3
Portion is fixedly connected, and the top movable of fixed plate 41 is connected with conical flask 42, and the both sides at the top of fixed plate 41 are fixed respectively to be connected
Support bar 43 is connected to, and the surface of conical flask 42 is connected with fixture block 44, and the side and the surface of support bar 43 of fixture block 44 are fixed
Connect, be fixedly connected with illuminating lamp 5 at the top of the inwall of casing 1, and the side of the inwall of casing 1 is fixedly connected with temperature controller
6, the side of casing 1 is connected with safety door 7, and the side of safety door 7 is fixedly connected with handle 8, and the two of the bottom of casing 1
Side has been fixedly connected with Convenient table 9.
During work, conical flask 42 is fixed in fixed plate 41 by fixture block 44, when shaking table 3 works constantly, conical flask 42 is more
Stable, this completes cell culture.
In summary
The biotechnology cell culture apparatus, it is fixedly connected by the bottom of the fixed plate 41 with the top of shaking table 3, and
The top movable of fixed plate 41 is connected with conical flask 42, and the both sides at the top of fixed plate 41 have been respectively fixedly connected with support bar 43, and
And the surface of conical flask 42 is connected with fixture block 44, the side of fixture block 44 is fixedly connected with the surface of support bar 43, change due to
Culture apparatus fixes unstable, the problem of loosening bottle stopper, it is therefore prevented that the pollution of miscellaneous bacteria in cell cultivation process, greatly reduces
The probability of cell culture failure；The biotechnology cell culture apparatus, by being fixedly connected with illumination at the top of the inwall of casing 1
Lamp 5, and the side of the inwall of casing 1 is fixedly connected with temperature controller 6, and the side of casing 1 is connected with safety door 7, and
And the side of safety door 7 is fixedly connected with handle 8, the single structure of in the market cell culture apparatus is changed, is considerably increased
Its feature, whole operation process are simple and convenient.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation make a distinction with another entity or operation, and not necessarily require or imply and deposited between these entities or operation
In any this actual relation or order.Moreover, term " comprising ", "comprising" or its any other variant are intended to
Nonexcludability includes, so that process, method, article or equipment including a series of elements not only will including those
Element, but also the other element including being not expressly set out, or it is this process, method, article or equipment also to include
Intrinsic key element.In the absence of more restrictions.By sentence " including one ... the key element limited, it is not excluded that
Other identical element in the process including the key element, method, article or equipment also be present ".
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (8)
1. a kind of biotechnology cell culture apparatus, including casing（1）, it is characterised in that：The casing（1）The side of inwall
It is fixedly connected with supporting plate（2）, and supporting plate（2）Top movable be connected with shaking table（3）, the shaking table（3）The two of top
Side has been fixedly connected with fixing device（4）.
A kind of 2. biotechnology cell culture apparatus according to claim 1, it is characterised in that：The fixing device
（4）Including fixed plate（41）, the fixed plate（41）Bottom and shaking table（3）Top be fixedly connected, the fixed plate（41）
The both sides at top have been respectively fixedly connected with support bar（43）.
A kind of 3. biotechnology cell culture apparatus according to claim 2, it is characterised in that：The fixed plate（41）
Top movable be connected with conical flask（42）, and conical flask（42）Surface be connected with fixture block（44）, the fixture block（44）'s
Side and support bar（43）Surface be fixedly connected.
A kind of 4. biotechnology cell culture apparatus according to claim 1, it is characterised in that：The casing（1）It is interior
LED illumination lamp is fixedly connected with the top of wall（5）.
A kind of 5. biotechnology cell culture apparatus according to claim 1, it is characterised in that：The casing（1）It is interior
The side of wall is fixedly connected with temperature controller（6）.
A kind of 6. biotechnology cell culture apparatus according to claim 1, it is characterised in that：The casing（1）'s
Side is connected with safety door（7）.
A kind of 7. biotechnology cell culture apparatus according to claim 6, it is characterised in that：The safety door（7）
Side be fixedly connected with handle（8）.
A kind of 8. biotechnology cell culture apparatus according to claim 1, it is characterised in that：The casing（1）Bottom
The both sides in portion have been fixedly connected with Convenient table（9）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710861615.7A CN107418895A (en) | 2017-09-21 | 2017-09-21 | A kind of biotechnology cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710861615.7A CN107418895A (en) | 2017-09-21 | 2017-09-21 | A kind of biotechnology cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107418895A true CN107418895A (en) | 2017-12-01 |
Family
ID=60433518
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710861615.7A Withdrawn CN107418895A (en) | 2017-09-21 | 2017-09-21 | A kind of biotechnology cell culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107418895A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107619787A (en) * | 2017-11-10 | 2018-01-23 | 安徽金思源生物科技有限公司 | A kind of biology laboratory uses constant-temperature table |
-
2017
- 2017-09-21 CN CN201710861615.7A patent/CN107418895A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107619787A (en) * | 2017-11-10 | 2018-01-23 | 安徽金思源生物科技有限公司 | A kind of biology laboratory uses constant-temperature table |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Pohlscheidt et al. | Optimizing capacity utilization by large scale 3000 L perfusion in seed train bioreactors | |
| CN106574249B (en) | Seed training method and application thereof | |
| CN203048951U (en) | Embedded multicell co-culture device | |
| EP1181548A4 (en) | Method for screening crystallization conditions in solution crystal growth | |
| JP6915992B2 (en) | Perfusion culture method and its use | |
| Kuan et al. | Growth optimization of Synechococcus elongatus PCC7942 in lab flasks and a 2‐D photobioreactor | |
| Darkins et al. | Design of large-scale manufacturing of induced pluripotent stem cell derived cardiomyocytes | |
| CN107418895A (en) | A kind of biotechnology cell culture apparatus | |
| CN102586190B (en) | CHO (Chinese Hamster Ovary) cell strain of efficiently-expressed recombinant human nerve growth factor and construction method thereof | |
| Hecht et al. | Efficiency improvement of an antibody production process by increasing the inoculum density | |
| EP2707387B1 (en) | A method of increasing the productivity of eucaryotic cells in the production of recombinant fviii | |
| BR0016561A (en) | Embryonic or similar cells to stem cells produced by cross-species nuclear transplants | |
| BR9908499A (en) | Embryonic or stem-like cell lines produced by cross-species nuclear transplantation | |
| Lee et al. | Recombinant antibody production by perfusion cultures of rCHO cells in a depth filter perfusion system | |
| Elliott | Nonperfused attachment systems for cell cultivation | |
| Kim et al. | Application of a cell‐once‐through perfusion strategy for production of recombinant antibody from rCHO cells in a Centritech Lab II centrifuge system | |
| EP1801201B1 (en) | Preparation of cells for production of biologicals | |
| KR100843131B1 (en) | Two-stage animal cell culture system equipped with depth filter | |
| CN105820990B (en) | A method of utilizing evolution target protein in iteration homologous recombination body | |
| WO2024019516A1 (en) | Method for culturing microalgae | |
| CN217781110U (en) | Multifunctional biological sample incubator | |
| Kutner et al. | Core facility for crystallographic and biophysical research to support the development of medicinal products | |
| CN1872997A (en) | New technique for culturing stem cells (carrying therapy gene) of human's xenogenic stromata in large-scale | |
| CN208776740U (en) | High dissolved oxygen glass shaking flask | |
| CN113846006B (en) | Wheat straw biological gas production microbial source analysis equipment based on PCR-DGGE technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20171201 |