CN107410223B - Small insect physiological behavior rhythm automatic monitoring and sampling device capable of being used for magnetic field treatment - Google Patents
Small insect physiological behavior rhythm automatic monitoring and sampling device capable of being used for magnetic field treatment Download PDFInfo
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- CN107410223B CN107410223B CN201710748176.9A CN201710748176A CN107410223B CN 107410223 B CN107410223 B CN 107410223B CN 201710748176 A CN201710748176 A CN 201710748176A CN 107410223 B CN107410223 B CN 107410223B
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Abstract
The invention discloses a small insect physiological behavior rhythm automatic monitoring and sampling device capable of being used for magnetic field treatment, which comprises an electromagnetic shielding cavity (26), wherein a full-time core rotary disc system is arranged on the electromagnetic shielding cavity (26), a rotating shaft (2) of the full-time core rotary disc system is vertically arranged in the middle of the electromagnetic shielding cavity (26), a base fixing support (31) is fixed at the far end of a rotary disc cantilever (13) which is uniformly distributed along the radial direction of the rotating shaft (2), a super-white glass feeding insect cover (12) is arranged above part of the base fixing support (31), a seedling feeding channel (14) and a seedling receiving channel (19) are respectively arranged on the front side and the rear side of the super-white glass feeding insect cover (12), and the rest part of the base fixing support (31) is positioned at the tail end of the seedling feeding channel (14) or at the starting end of the seedling receiving channel (19). The device can be matched with any external artificial magnetic field for use, fully automatically monitors, collects and analyzes ethological data, saves time and labor, and is accurate and easy to control.
Description
Technical Field
The invention relates to the technical field of sample collection and monitoring auxiliary equipment, in particular to an automatic monitoring device for animal physiological behavior rhythms, and specifically relates to an automatic monitoring and sample collection device for physiological behavior rhythms of small insects, which can be used for magnetic field treatment; the device greatly saves manpower and improves efficiency by combining a seedling feeding and collecting system with a full-time core turntable system; the environmental stability and the data readability are greatly improved by utilizing the environmental factor control and monitoring system and the insect testing activity monitoring system; electromagnetic interference is greatly reduced and the magnetic field processing accuracy is improved by utilizing the electromagnetic shielding cavity.
Background
The biological clock is an intangible 'clock' in the organism and carries the intrinsic rhythmicity of the life activity of the organism. At present, different periodic biological clocks of time, day, week, month, year and the like are reported in research, and the fact that the biological physiological and behavioral rhythms dominated by the biological clocks possibly play an important role in biological life activities is suggested. In human medical research, there are cases of treating cancer by combining human body's characteristics of circadian clock, and in education, there are also studies of teaching according to the characteristics of circadian clock rhythm of different people, with remarkable effect. Actually, the current researches on animals, plants, microorganisms and the like show that the daily life activities of organisms do not reflect specific rhythmicity, but for vector pests and crop pests, the researches on the rhythmical characteristics of the physiology and behaviors of the pests are helpful for making efficient prevention and treatment strategies according to the physiological and behavior characteristics of the pests at different times so as to reduce the harm of the pests.
Two methods of artificial observation and recording and video observation combined with software analysis are mainly used for researching insect biological clock and biological rhythm. From the aspect of behavioral research, the method of combining video observation and software analysis can basically meet the experimental requirements under the condition of not consuming more manpower. At present, partial gene pathways for regulating biological rhythm are preliminarily discovered by combining molecular biology technology, but for research of similar deep mechanisms, basic means such as manual time-by-time monitoring and timing sample collection by experimenters are generally needed, and in addition, for research of physiological rhythm, the experimenters are often needed to perform similar manual operations.
In view of the recent reports on the auxiliary tool for research related to regular collection of circadian rhythms and samples in the research of biological clocks, particularly on the increasingly important universal customized tool for cross-discipline- "biomagnetism" research, and the time and labor consumption and possible human errors of the research method relying on manual sample collection and observation and recording, a set of auxiliary device for research of biological circadian behaviors and rhythms, which is used for magnetic field processing and has the advantages of accurate and controllable environmental factors, automatic collection of samples in all periods and real-time monitoring of behaviors and activities, is urgently needed to be developed.
Disclosure of Invention
The invention aims to: the device fills the gap of the existing automatic collecting auxiliary equipment for the physiological behavior rhythm of the insect and the corresponding samples in different time periods, provides an integrated automatic monitoring and sample collecting device for the physiological behavior rhythm of the insect, which can be used for magnetic field treatment, saves labor, is efficient and stable, and has accurately controllable environmental factors (magnetic field, light source, temperature and humidity and gas characteristics), for the response research of the physiological behavior rhythm of the insect on the environmental factors.
The invention aims to solve the problems by the following technical scheme:
a small insect physiological behavior rhythm automatic monitoring and sampling device for magnetic field treatment comprises an electromagnetic shielding cavity, and is characterized in that: electromagnetic shield chamber on be equipped with environmental factor control and monitoring system, examination worm activity monitoring system, receive seedling system and full time period core carousel system for the seedling, the vertical setting in electromagnetic shield chamber's of full time period core carousel system's pivot middle part, the distal end of the radial evenly distributed's of pivot carousel cantilever is fixed with the base and holds in the palm, and be equipped with super white glass at the fixed position top that holds in the palm of partial base and raise the worm cover, be equipped with respectively at the front side and the rear side of super white glass raising the worm cover and give the seedling passageway and receive the seedling passageway, the fixed support of base of surplus portion can be located the tail end that gives the seedling passageway makes the sliding base that gets into through raising the worm passageway and can fall into in the fixed support of base or can be located the initiating terminal of receiving the seedling passageway and make the sliding base behind super white glass raising the worm cover can fall into and receive the seedling passageway.
The ultra-white glass insect feeding cover is arranged in the insect feeding cover fixing clamp, and the insect feeding cover fixing clamp is fixed on the channel bracket through the insect feeding cover fixing clamping arm; the super white glass feeding insect cover is provided with a seedling changing channel with a soft hairbrush, so that insects can not enter the super white glass feeding insect cover through the seedling changing channel.
A seedling collecting extension channel is arranged at the rear side of the seedling collecting channel, and the seedling feeding channel, the seedling collecting channel and the seedling collecting extension channel are fixedly arranged on the channel bracket; the tail end of the seedling feeding channel is provided with a seedling feeding switch, the starting end of the seedling collecting channel is provided with a seedling collecting switch, and the seedling feeding switch and the seedling collecting switch are respectively controlled by corresponding mechanical switch assemblies.
Slide base's inside cavity is in order to pack into seedling matrix, and is equipped with on the slide base and raises worm and use seedling fixed bolster, raises worm and uses seedling fixed bolster to fix at slide base through solid fixed ring with adjustable, and slide base can fix through base fixed switch and hold in the palm on the base is fixed.
The environment factor control and monitoring system comprises a full-spectrum LED light source, a filtering/polarizer component, an automatic humidifier, a sensor system, a gas release hole, a fan and a gas channel, wherein the full-spectrum LED light source is correspondingly arranged at the outer side of the filtering/polarizer component, corresponding positions of electromagnetic shielding cavities corresponding to a plurality of groups of full-spectrum LED light sources and the filtering/polarizer component are made of ultra-white glass, and the rest parts of the electromagnetic shielding cavities and a shell of the full-spectrum LED light source are made of aluminum materials to form a complete aluminum shell in a complementary mode, so that a Faraday cage effect is exerted; the automatic humidifier with the isosceles right triangle-shaped longitudinal section surrounds the upper part of the electromagnetic shielding cavity for a circle, and the surface of the automatic humidifier is provided with humidifying micropores; the sensor system is positioned on the rotating shaft at a position opposite to the height position of the ultra-white glass insect feeding cover and is arranged around the rotating shaft; the gas channel be located the below in electromagnetic shield chamber and be linked together with the lower part cavity hole of pivot, the lower part equipartition of pivot has gas release hole, is equipped with the fan in the bottom in electromagnetic shield chamber, the fan operation makes can abundant mixing through gas channel, gas release hole entering electromagnetic shield chamber's gas.
The sensor system comprises a light intensity meter, a temperature and humidity sensor, a fluxgate meter and a specific gas concentration meter, and is connected with the power supply and the control center through a data line so as to record data in real time and feed the data back to the power supply and the control center.
The power supply and control center is positioned outside the electromagnetic shielding cavity, and the environmental factor control and monitoring system is connected with the power supply and control center.
The insect testing activity monitoring system comprises an infrared variable-focus camera arranged on the upper part of the rotating shaft and behavior analysis software installed in a power supply and control center, wherein the infrared variable-focus camera is connected with the power supply and control center through a data line.
The rotating shaft is arranged on the top cover of the electromagnetic shielding cavity through a bearing, a stepping motor and a driver assembly are arranged at the top end of the rotating shaft to drive the rotating shaft to rotate, the stepping motor and the driver assembly are connected with a power supply and a control center through a circuit, and the rotary disc rotary arm is fixed on the rotating shaft through a rotating shaft adjusting ring; the stepping motor and driver assembly, the rotating shaft, the bearing, the rotating disc rotating arm, the rotating shaft adjusting ring, the sliding base fixing support and the power supply and control center form a full-time core rotating disc system.
The upper and lower part in electromagnetic shield chamber sets up a pair of dustcoat lock respectively, and the dustcoat lock can be opened respectively and fixed electromagnetic shield chamber upper portion and side in order conveniently to place or collect seedling and examination worm.
Compared with the prior art, the invention has the following advantages:
the device has the advantages of complete structure, complete functions, simple operation, accurate obtained data, small volume and strong open source property, can be matched with any external artificial magnetic field for use, and can be moved to a required place at any time, all components in the device are made of non-ferromagnetic materials, and the interference of ferromagnetic substances on the accuracy of an experiment for processing the artificial magnetic field is considered; the aluminum electromagnetic shielding cavity can effectively shield external medium-high frequency interference electromagnetic fields and electric fields, and the repeatability of magnetic field treatment experiments is improved; the environment factor control and monitoring system can provide specific light source characteristics (adjustable light period, light intensity, frequency, wavelength and polarized light source), temperature and humidity, magnetic field intensity and gas type and concentration according to experimental needs, can record corresponding characteristic parameters in real time, and provides a high-precision environment factor processing scheme; the insect testing activity monitoring system can record insect behavior activities in all weather, and can perform online analysis through behavior analysis software integrated by a power supply and a control center, so that the system is efficient and rapid; the full-time core turntable system is combined with the seedling receiving and feeding system, so that samples to be detected can be automatically collected in the full time according to requirements to perform subsequent physiological and molecular biology mechanism research, manpower is effectively reduced, human errors are reduced, and the experimental efficiency and the result accuracy are improved.
Drawings
FIG. 1 is a schematic longitudinal section structure diagram of an automatic monitoring and sampling device for physiological and behavioral rhythms of small insects in the invention;
FIG. 2 is a schematic cross-sectional structure diagram of the automatic monitoring and sampling device for the physiological and behavioral rhythms of the small insects of the invention;
FIG. 3 is a schematic diagram of a detailed structure of the insect feeding device of the present invention;
FIG. 4 is a detailed structure diagram of the seedling feeding channel and the seedling receiving channel of the present invention.
Wherein: 1-stepper motor and driver assembly; 2-a rotating shaft; 3-full spectrum LED light source; 4-a filter/polarizer assembly; 5, a bearing; 6, an automatic humidifier; 7-infrared zoom camera; 8-a sensor system; 9-feeding a seedling fixing bracket for the insects; 10-fixing a clamping arm by a pest feeding cover; 11-feeding insect cover fixing clamp; 12-ultra-white glass insect feeding cover; 13-rotating disc rotating arm; 14-a seedling feeding channel; 15-a sliding base; 16-channel support; 17-to Miao Kaiguan; 18-seedling collecting switch; 19-a seedling collecting channel; 20-a rotating shaft adjusting ring; 21-housing lock; 22-collecting the seedling to extend the channel; 23-gas release holes; 24-a fan; 25-gas channel; 26-electromagnetic shielding cavity; 27-power and control center; 28-changing the seedling channel; 29-a soft brush; 30-base fixed switch; 31-a base fixing support; 32-adjustable fixing ring; 33-mechanical switch assembly.
Detailed Description
The invention is further described with reference to the following figures and examples.
As shown in fig. 1-4: the utility model provides a small-size insect physiology behavioral rhythm automatic monitoring that can be used to magnetic field processing receives appearance device, includes electromagnetic shield chamber 26, the upper and lower part in electromagnetic shield chamber 26 sets up a pair of dustcoat lock 21 respectively, and dustcoat lock 21 can be opened respectively and fixed electromagnetic shield chamber 26 upper portion and side in order conveniently to place or collect seedling and examination worm. An environment factor control and monitoring system, a test insect activity monitoring system, a seedling feeding and collecting system and an all-time core rotary table system are arranged on an electromagnetic shielding cavity 26, a rotary shaft 2 of the all-time core rotary table system is vertically arranged in the middle of the electromagnetic shielding cavity 26, a base fixing support 31 is fixed at the far end of a rotary table cantilever 13 which is uniformly distributed along the radial direction of the rotary shaft 2, an ultra-white glass insect feeding cover 12 is arranged above the position of the partial base fixing support 31, the ultra-white glass insect feeding cover 12 is installed in an insect feeding cover fixing clamp 11, the insect feeding cover fixing clamp 11 is fixed on a channel support 16 through an insect feeding cover fixing clamp arm 10, and a seedling changing channel 28 with a soft brush 29 is arranged on the ultra-white glass insect feeding cover 12, so that insects cannot enter the ultra-white glass insect feeding cover 12 through the seedling changing channel 28; the front side and the rear side of the ultrawhite glass insect feeding cover 12 are respectively provided with a seedling feeding channel 14 and a seedling collecting channel 19, the rear side of the seedling collecting channel 19 is provided with a seedling collecting extending channel 22, the seedling feeding channel 14, the seedling collecting channel 19 and the seedling collecting extending channel 22 are fixedly arranged on a channel support 16, the tail end of the seedling feeding channel 14 is provided with a seedling feeding switch 17, the starting end of the seedling collecting channel 19 is provided with a seedling collecting switch 18, the seedling feeding switch 17 and the seedling collecting switch 18 are respectively controlled by corresponding mechanical switch assemblies 33, the rest part of the base fixing support 31 can be positioned at the tail end of the seedling feeding channel 14, so that the sliding base 15 entering through the seedling feeding channel 14 can fall on the base fixing support 31 or can be positioned at the starting end of the seedling collecting channel 19, so that the sliding base 15 passing through the ultrawhite glass insect feeding cover 12 can fall into the seedling collecting channel 19. The inside cavity of sliding seat 15 is in order to pack into the seedling matrix, and is equipped with on sliding seat 15 and raises worm with seedling fixed bolster 9, raises worm with seedling fixed bolster 9 and fixes at sliding seat 15 through adjustable solid fixed ring 32, sliding seat 15 can fix on the fixed support of base 31 through base stationary switch 30.
In the device, the environmental factor control and monitoring system comprises a full-spectrum LED light source 3, a filtering/polarizer component 4, an automatic humidifier 6, a sensor system 8, a gas release hole 23, a fan 24 and a gas channel 25, wherein the full-spectrum LED light source 3 is correspondingly arranged outside the filtering/polarizer component 4, corresponding positions of electromagnetic shielding cavities 26 corresponding to a plurality of groups of full-spectrum LED light sources 3 and the filtering/polarizer component 4 are made of ultra-white glass, and the rest parts of the electromagnetic shielding cavities 26 and the shell of the full-spectrum LED light source 3 are made of aluminum materials to form a complete aluminum shell in a complementary mode, so that a Faraday cage effect is exerted; the automatic humidifier 6 with the longitudinal section in the shape of an isosceles right triangle surrounds the upper part of the electromagnetic shielding cavity 26 for a circle, and the surface of the automatic humidifier 6 is provided with humidifying micropores; the sensor system 8 is positioned at a height position, facing the ultrawhite glass feeding insect cover 12, on the rotating shaft 2 and is arranged around the rotating shaft 2, the sensor system 8 comprises a light intensity meter, a temperature and humidity sensor, a fluxgate meter and a specific gas concentration meter, the sensor system 8 is connected with the power supply and control center 27 through a data line so as to record data in real time and feed the data back to the power supply and control center 27, the power supply and control center 27 is positioned outside the electromagnetic shielding cavity 26 so as to avoid electromagnetic field interference experiments, and the environmental factor control and monitoring system is connected with the power supply and control center 27; the gas channel 25 is located below the electromagnetic shielding cavity 26 and communicated with the lower hollow hole of the rotating shaft 2, the gas release holes 23 are uniformly distributed on the lower portion of the rotating shaft 2, the fan 24 is arranged at the bottom of the electromagnetic shielding cavity 26, and the fan 24 operates to enable gas entering the electromagnetic shielding cavity 26 through the gas channel 25 and the gas release holes 23 to be fully and uniformly mixed. The insect testing activity monitoring system comprises an infrared variable-focus camera 7 arranged on the upper part of the rotating shaft 2 and behavior analysis software installed in a power supply and control center 27, wherein the infrared variable-focus camera 7 is connected with the power supply and control center 27 through a data line. The rotating shaft 2 is arranged on a top cover of the electromagnetic shielding cavity 26 through a bearing 5, a stepping motor and driver assembly 1 is arranged at the top end of the rotating shaft 2 to drive the rotating shaft 2 to rotate, the stepping motor and driver assembly 1 is connected with a power supply and control center 27 through a circuit, and the rotary table swing arm 13 is fixed on the rotating shaft 2 through a rotating shaft adjusting ring 20; the stepping motor and driver assembly 1, the rotating shaft 2, the bearing 5, the rotary table swing arm 13, the rotating shaft adjusting ring 20, the sliding base fixing support 31 and the power supply and control center 27 form a full-time core rotary table system.
In the above device, the components in the electromagnetic shielding cavity 26 or mechanically connected with the electromagnetic shielding cavity 26 are all non-ferromagnetic glass fiber reinforced plastic, organic glass, resin or aluminum products, and the main magnetic generating devices such as the power generating device and the motor are all outside the electromagnetic shielding cavity 26, so as to reduce the influence on the artificial magnetic field when the experiment is carried out in the magnetic field.
The technical solution of the present invention is further illustrated by the following specific examples.
An insect physiological behavior rhythm automatic monitoring and sampling device for magnetic field treatment comprises an environmental factor control and monitoring system, an insect testing activity monitoring system, a seedling feeding and collecting system, a full-time core turntable system and an electromagnetic shielding frame body.
In the environmental factor control and monitoring system, the full-spectrum LED light sources 3 are all positioned at the outer sides of the three filtering/polarizer assemblies 4 to control the wavelength and the polarization characteristic of the light sources, the three groups of full-spectrum LED light sources 3 and the filtering/polarizer assemblies 4 are respectively positioned at the top and two sides of the electromagnetic shielding cavity 26, the corresponding positions of the electromagnetic shielding cavity 26 are made of ultra-white glass to provide good light transmittance, and the rest parts of the electromagnetic shielding cavity 26 and the shell of the full-spectrum LED light sources 3 are made of aluminum materials to form a complete aluminum shell in a complementary manner, so that the Faraday cage effect is exerted; the frequency, light period and light intensity of the full-spectrum LED light source 3 are regulated and controlled by a power supply and control center 27, and the filtering/polarizer component 4 is simultaneously or independently configured according to the experimental requirements to obtain a specific wavelength/polarized light source. The automatic humidifier 6 with the longitudinal section in the shape of an isosceles right triangle surrounds the upper part of the electromagnetic shielding cavity 26 for a circle, and the surface of the automatic humidifier 6 is provided with humidifying micropores; the sensor system 8 comprises a light intensity meter, a temperature and humidity sensor, a fluxgate meter and a specific gas concentration meter, the sensor system 8 is positioned at a height position on the rotating shaft 2 opposite to the ultrawhite glass feeding insect cover 12 and is arranged around the rotating shaft 2, and the sensor system 8 is connected with the power supply and control center 27 through a data line so as to record and feed back data to the power supply and control center 27 in real time; the gas channel 25 is located below the electromagnetic shielding cavity 26 and is communicated with the lower hollow hole of the rotating shaft 2, the gas release holes 23 are uniformly distributed on the lower portion of the rotating shaft 2, the fan 24 is arranged at the bottom of the electromagnetic shielding cavity 26, and the fan 24 operates to enable gas entering the electromagnetic shielding cavity 26 through the gas channel 25 and the gas release holes 23 to be fully mixed, so that the gas concentration is ensured to be accurate and controllable.
In the insect testing activity monitoring system, as shown in fig. 1: the infrared zoom camera 7 is positioned obliquely above the insect feeding device shown in fig. 3, corresponds to six groups of ultra-white glass insect feeding covers 12, focuses to the optimal visual field before the experiment, can monitor and record the behavior activities of the test insects day and night, and carries out statistical drawing analysis through behavior analysis software integrated in a power supply and storage control center 27.
In the seedling feeding and collecting system, the number of the seedling feeding channels 14 is twelve, the number of the seedling collecting channels 19 is six, except that the seedling feeding channels 14 and the seedling collecting channels 19 are not arranged at the positions corresponding to six groups of the ultrawhite glass feeding insect covers 12, two seedling feeding channels 14 are continuously arranged on the upper side of each group of the ultrawhite glass feeding insect covers 12 in the clockwise direction, and one seedling collecting channel 19 is arranged on the lower side of each group of the ultrawhite glass feeding insect covers 12 in the clockwise direction; the seedling feeding channel 14, the seedling collecting channel 19 and the seedling collecting extension channel 22 are supported by the channel bracket 16 and connected in series, and the seedling feeding channel 14 and the seedling collecting channel 19 can be just connected with the base fixing support 31 in a contact manner; the hollow interior of the sliding base 15 can be filled with a seedling matrix (nutrient solution or solid fertilizer), and the seedling fixing bracket 9 for feeding insects can be changed according to the seedling stem thickness and fixed on the sliding base 15 by an adjustable fixing ring 32; the full automation of a seedling feeding and collecting system is mainly realized by a seedling feeding switch 17, a seedling collecting switch 18, a base fixing switch 30 and a mechanical switch assembly 33, wherein a base fixing support 31 is driven by a rotary disc rotary arm 13 to rotate to a seedling feeding channel 14 and collide with Miao Kaiguan, a sliding base 15 and a test seedling thereon can slide down onto the base fixing support 31 and be fixed by the base fixing switch 30, the test seedling is rotated into an ultrawhite glass feeding insect cover 12 through a seedling changing channel 28 and a soft brush 29 which do not allow insects to escape so that the test insect can freely move and feed on the new seedling, after the set feeding time of the test insect, the rotary disc rotary arm 13 drives the base fixing support 31 and a feeding insect feeding seedling fixing support 9 on the sliding base 15 to a lower seedling collecting channel 19 again clockwise, the test seedling collecting switch 18 is impacted, the test insect treated seedling is collected by matching with the mechanical switch assembly 33, and the test insect treated seedling is made to slide into a seedling collecting extending channel 22 through the seedling collecting channel 19 so as to carry out subsequent test insect yield, egg yield, physiological molecular research and physiological molecular feeding mechanism or physiological research.
The core rotary disc system in the whole time period runs clockwise, the power source is the stepping motor and the driver component 1, the rotary shaft 2 fixed by the bearing 5 is linked to rotate according to the set time interval of the power supply and storage control center 27, the rotary disc rotary arm 13 drives the base fixing support 31, and the seedling feeding and receiving system is integrated to fully automatically complete the seedling feeding, receiving and changing work. The spindle adjustment ring 20 calibrates the level of the turntable according to the customized height of the base fixing bracket 31 to provide more accurate experimental assistance.
The electromagnetic shielding frame body comprises an aluminum electromagnetic shielding cavity 26 and an aluminum shell part of the full-spectrum LED light source 3, the upper half part of the outer cover lock 21 can be opened as required, the outer cover lock is moved out together with the rotary table by opening the rotating shaft adjusting ring 20 so as to be convenient to maintain, meanwhile, the sliding base 15 with test seedlings is conveniently placed in the seedling feeding channel 14, and the test insects can be conveniently placed in the ultra-white glass feeding insect cover 12; the lateral part of the cover lock 21 can be opened as required to collect the test insects treated seedlings positioned in the seedling collecting extension channel 22 after the whole rhythm experiment is finished, so as to be used for carrying out subsequent physiological and molecular biology experiments.
The device has the advantages of complete structure, complete functions, simple operation, accurate obtained data, small volume and strong open source property, can be matched with any external artificial magnetic field for use, and can be moved to a required place at any time, all components in the device are made of non-ferromagnetic materials, and the interference of ferromagnetic substances on the accuracy of an experiment for processing the artificial magnetic field is considered; the aluminum electromagnetic shielding cavity 26 can effectively shield external medium-high frequency interference electromagnetic fields and electric fields, and the repeatability of magnetic field treatment experiments is improved; the environment factor control and monitoring system can provide specific light source characteristics (adjustable light period, light intensity, frequency, wavelength and polarized light source), temperature and humidity, magnetic field intensity and gas type and concentration according to experimental needs, can record corresponding characteristic parameters in real time, and provides a high-precision environment factor processing scheme; the insect testing activity monitoring system can record insect behavior activities in all weather, and can perform online analysis through behavior analysis software integrated by the power supply and the control center 27, so that the system is efficient and rapid; the full-time core turntable system is combined with the seedling receiving and feeding system, so that samples to be detected can be automatically collected in the full time according to requirements to perform subsequent physiological and molecular biology mechanism research, manpower is effectively reduced, human errors are reduced, and the experimental efficiency and the result accuracy are improved.
The above embodiments are only for illustrating the technical idea of the present invention, and the protection scope of the present invention cannot be limited thereby, and any modification made on the basis of the technical scheme according to the technical idea proposed by the present invention falls within the protection scope of the present invention; the technology not related to the invention can be realized by the prior art.
Claims (7)
1. An automatic monitoring and sampling device for physiological and behavioral rhythms of small insects, which can be used for magnetic field treatment, comprises an electromagnetic shielding cavity (26), and is characterized in that: the device is characterized in that an environmental factor control and monitoring system, a test insect activity monitoring system, a seedling feeding and collecting system and a full-time core rotary table system are arranged on the electromagnetic shielding cavity (26), a rotating shaft (2) of the full-time core rotary table system is vertically arranged in the middle of the electromagnetic shielding cavity (26), base fixing supports (31) are fixed at the far ends of rotary table cantilevers (13) which are uniformly distributed along the radial direction of the rotating shaft (2), ultra-white glass feeding insect covers (12) are arranged above the positions of part of the base fixing supports (31), a seedling feeding channel (14) and a seedling collecting channel (19) are respectively arranged on the front side and the rear side of each ultra-white glass feeding insect cover (12), and the rest part of the base fixing supports (31) can be positioned at the tail end of the seedling feeding channel (14) to enable a sliding base (15) entering through the seedling feeding channel (14) to fall on the base fixing supports (31) or can be positioned at the starting end of the seedling collecting channel (19) to enable the sliding base (15) after passing through the ultra-white glass feeding insect covers (12) to fall into the seedling collecting channel (19); the ultra-white glass insect feeding cover (12) is arranged in an insect feeding cover fixing clamp (11), and the insect feeding cover fixing clamp (11) is fixed on the channel bracket (16) through an insect feeding cover fixing clamping arm (10); a seedling changing channel (28) with a soft brush (29) is arranged on the ultra-white glass insect feeding cover (12), so that insects cannot enter the ultra-white glass insect feeding cover (12) through the seedling changing channel (28); a seedling collecting extension channel (22) is arranged at the rear side of the seedling collecting channel (19), and the seedling feeding channel (14), the seedling collecting channel (19) and the seedling collecting extension channel (22) are fixedly arranged on the channel bracket (16); the tail end of the seedling feeding channel (14) is provided with a feeding Miao Kaiguan (17), the starting end of the seedling collecting channel (19) is provided with a seedling collecting switch (18), and the feeding Miao Kaiguan (17) and the seedling collecting switch (18) are respectively controlled by corresponding mechanical switch components (33); the inside cavity of sliding base (15) is in order to pack into the seedling matrix, and is equipped with on sliding base (15) and raises worm and fix support (9) with the seedling, raises worm and fixes support (9) with the seedling through solid fixed ring (32) with adjustable and fix sliding base (15), and sliding base (15) can be fixed on base fixed support (31) through base fixed switch (30).
2. The device for automatically monitoring and collecting the circadian rhythm of small insects for magnetic field treatment according to claim 1, wherein: the environment factor control and monitoring system comprises a full-spectrum LED light source (3), a filtering/polarizer component (4), an automatic humidifier (6), a sensor system (8), a gas release hole (23), a fan (24) and a gas channel (25), wherein the full-spectrum LED light source (3) is correspondingly arranged at the outer side of the filtering/polarizer component (4), corresponding positions of electromagnetic shielding cavities (26) corresponding to a plurality of groups of full-spectrum LED light sources (3) and the filtering/polarizer component (4) are made of ultra-white glass, and the rest parts of the electromagnetic shielding cavities (26) and the shell of the full-spectrum LED light source (3) are made of aluminum materials to form a complete aluminum shell in a complementary mode, so that the Faraday cage effect is exerted; the automatic humidifier (6) with the longitudinal section in an isosceles right triangle surrounds the upper part of the electromagnetic shielding cavity (26) for a circle, and the surface of the automatic humidifier (6) is provided with humidifying micropores; the sensor system (8) is positioned at the position of the rotating shaft (2) at the height position opposite to the ultrawhite glass feeding insect cover (12) and is arranged around the rotating shaft (2); gas passage (25) be located the below of electromagnetic shield chamber (26) and be linked together with the lower part cavity hole of pivot (2), the lower part equipartition of pivot (2) has gas release hole (23), is equipped with fan (24) in the bottom of electromagnetic shield chamber (26), fan (24) operation makes and can fully mix through gas passage (25), gas release hole (23) entering electromagnetic shield chamber (26) gas.
3. The device for automatically monitoring and collecting the circadian rhythm of small insects for magnetic field treatment according to claim 2, wherein: the sensor system (8) comprises a light intensity meter, a temperature and humidity sensor, a fluxgate meter and a specific gas concentration meter, and the sensor system (8) is connected with the power supply and control center (27) through a data line so as to record data in real time and feed the data back to the power supply and control center (27).
4. The automatic monitoring and sampling device for the physiological and behavioral rhythm of small insects applicable to magnetic field treatment according to claim 3, wherein: the power supply and control center (27) is positioned outside the electromagnetic shielding cavity (26), and the environmental factor control and monitoring system is connected with the power supply and control center (27).
5. The device for automatically monitoring and collecting the circadian rhythm of small insects for magnetic field treatment according to claim 1, wherein: the insect test activity monitoring system comprises an infrared variable-focus camera (7) arranged on the upper part of the rotating shaft (2) and behavior analysis software installed in a power supply and control center (27), wherein the infrared variable-focus camera (7) is connected with the power supply and control center (27) through a data line.
6. The device for automatically monitoring and collecting the circadian rhythm of small insects for magnetic field treatment according to claim 1, wherein: the rotating shaft (2) is mounted on a top cover of the electromagnetic shielding cavity (26) through a bearing (5), a stepping motor and a driver assembly (1) are arranged at the top end of the rotating shaft (2) to drive the rotating shaft (2) to rotate, the stepping motor and the driver assembly (1) are connected with a power supply and control center (27) through a circuit, and the rotary table swing arm (13) is fixed on the rotating shaft (2) through a rotating shaft adjusting ring (20); the stepping motor and driver assembly (1), the rotating shaft (2), the bearing (5), the rotary table rotating arm (13), the rotating shaft adjusting ring (20), the sliding base fixing support (31) and the power supply and control center (27) form a full-time core rotary table system.
7. The small insect circadian rhythm automatic monitoring and sampling device for magnetic field treatment according to claim 1, characterized in that: the upper and lower part of electromagnetic shield chamber (26) sets up a pair of dustcoat lock (21) respectively, and dustcoat lock (21) can be opened respectively and fixed electromagnetic shield chamber (26) upper portion and side in order conveniently to place or collect seedling and examination worm.
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