CN107312802A - A kind of maize straw synchronous fermentation process - Google Patents
A kind of maize straw synchronous fermentation process Download PDFInfo
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Abstract
The invention discloses a kind of maize straw synchronous fermentation process, comprise the following steps：Collect also stalk of the water content without aging more than 50% and be placed in cryogenic conditions sealing preserve after 3 months, take out, be crushed to the corn stalk pelletses that particle diameter is 2.5cm；After being soaked using 1.5%NaOH solution to corn stalk pelletses, progress alkali formula extractive reaction in water bath with thermostatic control is placed in；After completion of the reaction, products therefrom is washed to pure water and be placed in after neutral pH, drying in the reaction system that initial substrate concentration is 1g/100mL, under conditions of 36 DEG C, after 80rmp per minute speed sync diastatic fermentation 96h, centrifugation, hydrolyzate is obtained, the detoxification treatment of hydrolyzate is completed.The present invention, so as to obtain alcohol fuel, also adds the benefit of society simultaneously using the Production in Straw Fermentation alcohol of crop waste, the problem of solving crop straw burning pollution environment, and by the optimization of technological parameter, substantially increases the yield of ethanol.
Description
Technical field
The present invention relates to biomass energy preparation field, and in particular to a kind of maize straw synchronous fermentation process.
Background technology
Energy crisis is just quietly stepped among the sight of the mankind before decades, along with the middle of last century
Energy crisis, energy reserves in the world is just in dramatic decrease, and for multiplying and living for our mankind, each state is all in renewable energy
Source this part has put into sizable energy.Because the energy is exactly the guarantee that the mankind can persistently live down.Although petroleum resources
Storage level is very big, but it is limited after all, and the oil price that competitively goes up from oil exporting country is it can be seen that the problem of energy shortage
It is increasingly nearer from us.Combustible ice and solar energy resources are always the focus of scientists from all over the world's research, but up to the present, open
Degree of raising difficult questions is still very big, and the use cost of great number also allows many people to hang back.Between nearest decades, scientist will regard
Line is transferred to this field of biomass energy, and biomass energy is well-known clean energy resource, and many countries are by biology
Mass-energy source is applied to automobile and domestic environments.Biomass energy can exist with three kinds of forms of gas-liquid-solid, be that it is widely used
There is provided good premise.
Ethanol is one of common biomass energy, and indispensable effect is played in today's society, season is gathered in every year
Waste straw often can for carry out ethanol production.Ethanol is clean energy resource as combustible ice and solar energy, is being opened
Environmental pollution very little when hair is utilized, it might even be possible to reach pollution-free.The progress of maize straw fermentation production alcohol technique is such as
Among the exploration of the fire such as bitter edible plant.But being intended to carry out commercialized large-scale production still has many problems, how to improve ethanol
Rate and acquisition high conversion have been the focuses of research.After season is gathered in every year by China, the processing side of maize straw is discarded
The problem of formula always is one unavoidably, the today increasingly paid attention in environmental problem, countries in the world are to new energy development
This field has all put into sizable dynamics.Maize straw is generally burned or natural air drying in man of peasant household using direct collective
The mode sold is handled, and both modes are not all utilized efficiently to maize straw.The only annual corn of China
Stalk yield all reaches more than one hundred million tons, if these maize straws can be carried out to fermentation production alcohol, then the alcohol production of annual China
Amount can significantly rise, it can be seen that the Research Prospects of maize straw are very wide.
China is the big country of energy resource consumption, and it is the producing and ethanol big country taken its place in the front ranks of the world all the time to be also, in view of China's energy
It is in short supply, the Devoting Major Efforts To Developing of this new energy of ethanol can solve the matter of great urgency.The total output of China's country's ethanol can reach every year
To 1,400,000 tons, during all ethanol plants production ethanol, account for quite a lot of using starchiness class and molasses class raw material
Ratio.The continuous breakthrough studied with China scientist in terms of biomass energy, maize straw is thought by scientist
Be can low cost produce in enormous quantities ethanol important materials, with the continuous expansion and improvement of production technology, utilize corn stalk
The zymotechnique of cellulose raw producing and ethanol in stalk is more and more ripe, and there be entering for brilliance in domestic major colleges and universities and scientific research institutions
Exhibition.
And the harvesting of maize straw is the first step of whole technique, this relates to corn straw harvesting time and storage side
A series of problems of formula.Agricultural crop straw plantation is universal all to have seasonality, generally all can not disposably utilize, how to be deposited
Storage and storage are one the problem of influence its internal fiber plain conversion ratio some months.If adopted on harvest time and harvesting approach
With the mode for being conducive to subsequent fermentation technological process, it can just make again in stalk pretreatment and the enterprising one-step optimization of hydrolysing step
Obtaining stalk raw material can more fully be utilized.It is rural area harvesting that outdoor lower solar exposure to stalk water content, which is minimized,
More typical waste straw processing method after season, the maize straw in rural area generally uses this simple mode, and this is due to
Waste straw is more, and collecting wastes time and energy, and typically after crops are gathered in, uniformly collects and is dried, Zhi Daoyu
The water content of rice stalk is less than 10%.According to weather and environmental difference, time of storage is also depending on weather.In the mistake of dry storage
Cheng Zhong, the loss amount of dry is about 15%, and stalk belongs to inflammable articles, and safety problem is also need to consider one
Ring.Wet method green grass or young crops storage is a kind of storage mode for being different from dry storage, is a newer side in waste straw pre-treating technology
Method.Specific method is to collect the maize straw also without arid aging, should ensure that in stalk water content more than 50% or more
Many, sealing preserve after the maize straw for meeting this condition is collected is unsealed and used again after storage certain time.It is wet storage be
Storage is directly collected after maize straw is discarded, this can improve the harvesting efficiency of stalk, it is not necessary to wait the time dried, simultaneously
The homogeneity of raw material is also increased, the loss of dry in stalk is preferably minimized.Thus particle diameter, time and storage mode are all
Influence can be produced on the technique of maize straw synchronous fermentation, the analysis different pre-treatments mode of system is to maize straw zymotechnique
Influence it is necessary.
Simultaneous saccharification and fermentation method by raw material of maize straw produce ethanol technological process in show excellent, it with the past
Fermentation process it is different, this method can farthest reduce the feedback inhibition in overall reaction system.It allows maize straw
Hydrolysis and ferment in same system carry out, in this reaction system, cellulase using maize straw be substrate production
Ethanol is directly turned into by fermentation by saccharomyces cerevisiae after sugar.Avoid excessive concentration and the feedback inhibition produced to reaction and processing
The problem of a large amount of accessory substances during stepwise reaction.Whole technological process only has a step, and this causes the utilization rate of equipment to get a promotion,
The reaction time of whole technique is shortened, this method is particularly necessary from the point of view of long-range economic benefit.
The content of the invention
To solve the above problems, the invention provides a kind of maize straw synchronous fermentation process, utilizing crop waste
Production in Straw Fermentation alcohol, so as to obtain alcohol fuel, also adds the benefit of society simultaneously, solves crop straw burning pollution ring
The problem of border, and by the optimization of technological parameter, substantially increase the yield of ethanol.
To achieve the above object, the technical scheme taken of the present invention is：
A kind of maize straw synchronous fermentation process, it is characterised in that comprise the following steps：
S1, collection also stalk of the water content without aging more than 50% were placed in cryogenic conditions sealing preserve after 3 months,
Take out, be crushed to the corn stalk pelletses that particle diameter is 2.5cm；
S2, using 1.5%NaOH solution the corn stalk pelletses of gained are soaked, be subsequently placed in 80 DEG C of waters bath with thermostatic control
Under the conditions of reaction 1h carry out alkali formula extractive reaction；After completion of the reaction, products therefrom is washed to neutral pH with pure water, then carried out
Drying and processing；
S3, take brewer's dried yeast appropriate, dissolved in the ratio that OD values are 2, bacterium solution is then transferred to what is configured
Cultivated on potato culture medium, after bacterium is covered with thereon, carry out tube purifying, continue cultivate, it is to be purified after saccharomyces cerevisiae
Just cover with after slant tube culture medium, it is standby；
S4, the maize straw obtained by step S2 is placed in the reaction system that initial substrate concentration is 1g/100mL, fiber
Plain enzyme dosage is 30FPU/g, and beta-glucosidase enzyme dosage is 15FPU/g, and adding saccharomyces cerevisiae makes the OD values of yeast in system be
2, sodium citrate regulation pH is 4.8, then under conditions of 36 DEG C, with 80rmp per minute speed sync diastatic fermentation 96h,
After the completion of fermentation, using supercentrifuge with 10000r/min centrifugation 5min, maize straw residue precipitation is discarded, water is obtained
Solve liquid；
S5, the in mass ratio 1% addition proper amount of active carbon in the hydrolyzate of gained, so as to complete at the detoxification of hydrolyzate
Reason.
The present invention, so as to obtain alcohol fuel, is also added simultaneously using the Production in Straw Fermentation alcohol of crop waste
The benefit of society, the problem of solving crop straw burning pollution environment, and by the optimization of technological parameter, substantially increase ethanol
Yield.
Brief description of the drawings
Fig. 1 is the glucose standard curve in the embodiment of the present invention.
Fig. 2 is the ethanol standard curve in the embodiment of the present invention.
Fig. 3 is influence of the different-grain diameter condition to alcohol absorbance in the embodiment of the present invention.
Fig. 4 is influence of the different storage times to alcohol absorbance in the embodiment of the present invention.
Fig. 5 influences for different storage modes in the embodiment of the present invention on alcohol absorbance
Fig. 6 is the front and rear influence to alcohol absorbance of activated carbon detoxification in the embodiment of the present invention.
Embodiment
In order that objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further
Describe in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair
It is bright.
Maize straw used originates from the station economic and technological development zone of Jilin Province Jilin nine rural area nearby in following examples.It is logical
Cross the measure that following methods complete three kinds of main components of maize straw：
(1) content of cellulose is determined
1. material prepares：Take that the maize straw sample before and after pretreatment is appropriate, pulverizer pulverizes and sieves, accurate weighing sieving
Maize straw sample 0.1g afterwards is added in conical flask, is added equal proportion acetic acid and the common 5mL of nitric acid mixed liquor, is placed in boiling water bath
Middle heating 30 minutes, water-bath is cooled down after finishing, then is all gone in 50mL centrifuge tubes, adds ultra-pure water to mend during less than 45mL
Together, it is placed in low speed centrifuge with 4000r/min centrifugation 20 minutes, precipitation is taken after centrifugation, will be after precipitation washing three times
It is placed in drying for standby in air dry oven.
2. assay：Sample after drying is placed in conical flask, plus the sulfuric acid and 10mlL that 10mL mass fractions are 10%
0.1mol/L potassium bichromate solution, shake up, boiling water bath 10 minutes.After cooling plus volume for 5mL 5% KI it is molten
Liquid and 1% starch solution that volume is 1mL, the Na for being 0.2mol/L with mass fraction after system mixing completely2S2O3It is titrated to
Solution just shows blueness and solution nondiscolouring within half a minute.It is another to do a blank control for not adding stalk.
3. content of cellulose is calculated：
X=T (a1-a2)/(m*24) (1)
Wherein：The concentration of T- sodium thiosulfate, unit is mol/L；a1The Na that-blank titration is consumed2S2O3Volume,
Unit is mL；a2The Na that-solution is consumed2S2O3Volume, unit is mL；The quality of m- maize straws；“24”-1mol
C6H10O5Corresponding Na2S2O3Molar fraction.
(2) hemicellulose level is determined
1. material prepares：Take that the maize straw sample before and after pretreatment is appropriate, pulverizer pulverizes and sieves, accurate weighing sieving
Maize straw sample 0.1g afterwards is added in conical flask, is added 80% calcium nitrate solution that volume is 10mL, is shaken up rearmounted
Let cool after boiling water bath is heated 5 minutes, add distilled water diluting solution and filter residue, residue is washed 3~5 times with distilled water,
It is placed in dry for standby in air dry oven.Sample after drying adds 10ml 2mol/L hydrochloric acid, and boiling water bath 45 minutes treats that solution is complete
One is added dropwise after full cooling and drips phenolphthalein indicator, then with 2mol/L NaOH and solution is to rosiness, is then filtered, and water
Filter wash slag, last merging filtrate and cleaning solution survey volume.
2. the making of glucose standard curve：1.0mg/mL glucose standards and distilled water are added by each test tube of table 1,
As a result it is as shown in Figure 1.
The configuration of the standard curve of table 1
3. assay：1mL liquid to be determined is taken in scale test tube, the DNS that can be used after placing 7 days is added molten
Liquid 2mL, it is well mixed to be placed in boiling water heating 5 minutes in water-bath afterwards, finish relief test tube and be cooled to room temperature, add distillation
Water is settled to 15mL, and in light-metering absorption value under ultraviolet specrophotometer 540nm wavelength, three light absorption values of every group of parallel determination will
The amount that formula calculates reduced sugar is substituted into after results averaged, the result after calculating finally is multiplied by into 0.9 can obtain half fibre
Tie up the amount of element.
(3) content of lignin is determined
1. material prepares：Take that the maize straw sample before and after pretreatment is appropriate, pulverizer pulverizes and sieves, accurate weighing sieving
Maize straw sample 0.1g afterwards is added in conical flask, is added 1% acetum that cumulative volume is 10mL, is soaked 30 minutes
Filtering, washs filter residue once, the dry for standby in air dry oven with 1% acetum.Sample after drying is put into equal volume
Ethanol and ether mixed liquor amount to 4mL and carry out immersion treatment, testing liquid centrifuging and taking is precipitated after 5 minutes, by sediment
Washed 3~5 times with distilled water, washing is added after finishing will be molten after 73% sulfuric acid solution that cumulative volume is 3mL, gentle agitation
Liquid is placed 16 hours, adds volume for 10mL water, is placed in water-bath and is heated 3~5 minutes, finally adds the steaming that volume is 5mL
Distilled water and 10% barium chloride solution that volume is 0.5mL, centrifuging and taking precipitation wash the precipitating residue after centrifugation 2~3 times,
Transposition is in air dry oven dry for standby.
2. assay：Sample after drying is placed in conical flask, plus the sulfuric acid and 10mL that 10mL mass fractions are 10%
Concentration is 0.1mol/L potassium bichromate solution, is shaken up, boiling water bath 10 minutes.After cooling plus volume be 5mL 5% iodate
Potassium solution and 1% starch solution that volume is 1mL, the Na for being 0.2mol/L with mass fraction after system mixing completely2S2O3Drop
It is fixed just to have shown blue and solution nondiscolouring within half a minute to solution.It is another to do a blank control for not adding stalk.
3. content of lignin is calculated：
X=T (a1-a2)/(m*48) (2)
Wherein：T-Na2S2O3Concentration, unit is mol/L；a1The Na of-blank titration consumption2S2O3Volume, unit is
mL；a2- solution consumption Na2S2O3Volume, unit is mL；The quality of m- maize straws, unit is g；“48”-1mol C11H12O4
Corresponding Na2S2O3Molar fraction.
Embodiment
Step 1: the pre-treatment of maize straw
(1) different-grain diameter size is handled：By maize straw according to certain size comminution to 2.5cm, 1.5cm, 0.5cm.
(2) different storage time processing：Harvested maize straw is dried into rear room temperature storage 3 months, 6 months, 12
Individual month.
(3) different storage mode processing：Including not crushing dry storage (stalk of collection is placed in into open-air seasoning)；Crush dry
Store (being placed in open-air seasoning after the stalk of collection is carried out into different degrees of crushing on demand)；Wet storage is not crushed (to collect also
There is no stalk of the water content of aging more than 50% to be placed in cryogenic conditions sealing preserve)；Wet storage is crushed (by the not old of collection
The maize straw of change is placed in cryogenic conditions sealing preserve again after crushing on request).
Step 2: being soaked using 1.5%NaOH solution to the maize straw after pre-treatment, 80 DEG C of constant temperature are subsequently placed in
1h is reacted under water bath condition and carries out alkali formula extractive reaction.Sample after alkali process with pure water is washed to neutrality by question response after finishing
PH, then for simultaneous saccharification and fermentation and composition measurement after drying and processing.
Step 3: taking, brewer's dried yeast is appropriate, is dissolved in OD values for 2 ratio, is then transferred to bacterium solution and matches somebody with somebody
Cultivated on the potato culture medium put, after bacterium is covered with thereon, carry out tube purifying, continue cultivate, it is to be purified after wine
Brewer yeast is just covered with after slant tube culture medium, configures saccharomyces cerevisiae bacterium solution, prepares to be used for synchronous fermentation.
Step 4: the maize straw sample obtained by step 2 is placed in into the reactant that initial substrate concentration is 1g/100mL
System, cellulase consumption 30FPU/g, beta-glucosidase enzyme dosage 15FPU/g, adding saccharomyces cerevisiae makes the OD values of yeast in system
For 2, sodium citrate regulation pH is 4.8, then under conditions of 36 DEG C, with 80rmp per minute speed sync diastatic fermentation, body
The total reaction time of system is 96h, in the sampling of certain time point, using supercentrifuge with 10000r/min centrifugation
5min, discards maize straw residue precipitation, obtains hydrolyzate, the content of ethanol in hydrolyzate is then determined afterwards.
Step 5: in the hydrolyzate of gained the in mass ratio 1% appropriate activated carbon of addition, so as to realize the de- of hydrolyzate
Poison processing.The measure of ethanol content
(1) principle：This experiment determines the content of alcohol in zymotic fluid using potassium dichromate method.Ethanol is dissolving in sulfuric acid solution
After green product (trivalent chromium) can be quantitatively oxidized to by potassium bichromate.The a length of 600nm of maximum absorption wave of oxidation product, it is inhaled
Luminosity and contained concentration of alcohol are in direct ratio.
The configuration of (2) 5% potassium bichromate solutions：Accurately weigh 5.00g potassium bichromates to be dissolved in 50mL water, then add
The 10mL concentrated sulfuric acid, after cooling down after solution reaction and completely, adds water and is settled to 100mL.
(3) ethanol standard liquid：Absolute ethyl alcohol 0.25mL is accurately shifted using liquid-transfering gun into 100mL volumetric flasks and fixed
Hold.Now in solution per milliliters of liquid equivalent to 2.0mg ethanol.
(4) drafting of standard curve：By the configuration standard solution of table 2
The making of the ethanol standard curve of table 2
Often pipe adds 5% potassium bichromate solution that volume is 2mL, and distilled water is added to scale.Boiling water bath heating ten minutes, so
Cooled down afterwards with flowing water, using a pipe as reference, absorbance is determined at 600nm, made with concentration of alcohol and correspondence absorbance
Standard curve (Fig. 2).
(5) sample analysis：Sample 1mL after centrifugation is taken in colorimetric cylinder, to add 2.0mL 5% potassium bichromate solution, add water
To scale, blank tube still for No. one pipe, 600nm determine absorbance, each absorbance do three groups it is parallel.Finally according to absorbance
Average value find the content (mg) of ethanol.Sample volume is 1mL, of the total volume 1/10, so being needed when calculating ethanol content
It is multiplied by 10.
As a result with analysis
Constituent analysis as shown in table 3, as a result shows that the cellulose rate of recovery is higher under this condition, can reach 94.32%,
Hemicellulose fraction dissolution, the rate of recovery is 61.91%, and lignin can largely be removed, and removal rate is 56.82%.
Table 3 pre-processes front and rear composition measurement
Under the conditions of 0.5cm, 1.5cm, 2.5cm particle diameter, alcohol absorbance change is as shown in Figure 3 during synchronous fermentation：It is beautiful
Under the conditions of the contrast that rice stalk is handled before three kinds, the amount of the maize straw production alcohol of 2.5cm particle sizes is most.This is probably
Because corn stalk powder is broken to after different-grain diameter size, storage and follow-up pretreatment and the process of enzyme hydrolysis are all generated
Very big influence.Heat transfer is influenceed by medium size, therefore the heat transfer rate under three kinds of different-grain diameters can be variant, and this can make not
Reaction speed with the conditions of is different, if so can be contributed to using more suitable particle size progress pre-treatment
The yield of glucose after the efficiency and enzyme hydrolysis that maize straw is pre-processed in subsequent technique is improved, glucose yield raises it
Afterwards, the amount for being converted into ethanol is consequently increased, and then reduces the cost of Production in Straw Fermentation alcohol.Principle therein is in pretreatment
When, it is unbalanced due to heat and the speed of mass transfer, it is possible to cause coarse biomass outer surface heated excessive, one
A little accessory substances increase therewith, and this can make its internal-response insufficient, or even unreacted；And for small particle biomass, it
The material such as hemicellulose may be too small because of particle diameter, whole course of reaction rapidly, the more violent mistake that reaction is carried out
Degree degrades material therein, ultimately results in the destruction of its structure serious, it is impossible to obtain expected product.As can be seen here, systematically
Development of the difference to cellulose ethanol process produced by analysis different-grain diameter size is particularly necessary.
Storage time is that under conditions of 3 months, 6 months, 12 months, alcohol absorbance change is as schemed during synchronous fermentation
Shown in 4：Under three kinds of different storage times, storage time was better than other two groups, storage for the maize straw ethanol production of 3 months
Time is that yield of the maize straw ethanol production of 6 months also than 12 months is slightly higher, it can be seen that with the extension of time, corn stalk
The yield of stalk fermentation alcohol is decreased.This is probably due to the extension of storage time, it is generated inside maize straw
It has the product of inhibitory action to zymotechnique, and these materials have inhibition to subsequent technique, so being unfavorable for maize straw
Further reaction.It is also likely to be the internal fiber structure due to the extension of time, the water content of maize straw changes
Change, the yield of final influence fermentation production alcohol.It is also possible to being due to that stalk is constantly in sunlight in the drying process
The process being exposed to the sun, generates accessory substance under conditions of long-time high temperature, is unfavorable for subsequent reactions.
Storage mode does not crush dry storage not crush wet storage, crushes wet storage, under the conditions of crushing dry storage, stalk
Alcohol absorbance change is as shown in Figure 5 during synchronous fermentation：The maize straw yield of wet method green grass or young crops storage processing is than directly doing storage
The effect deposited is good, and main cause is probably because the maize straw of wet storage maintains the flexible fiber construction of itself, this knot
Structure is more preferably beneficial to the progress of hydrolysis and fermentation.And the stalk accumulation do not crushed is loose, is circulated beneficial to gas, gives microorganism growth
There is provided condition.Microorganism can produce influence by katabolism on stalk internal structure under suitable condition.So wet method is protected
Deposit and preserved better than dry method, pulverization process is not better than pulverization process.
To clearly show that influence of the synchronous fermentation liquid through being produced before and after detoxification treatment to it, several groups of maize straws are randomly selected
Zymotic fluid contrasted before and after detoxification, then by results averaged.As shown in fig. 6, the curve in figure clearly present it is same
The influence produced before and after step diastatic fermentation liquid detoxification to it, it is seen that simultaneous saccharification and fermentation liquid is follow-up anti-to it after detoxification treatment
The progress effect of being very helpful answered, while being also beneficial to the lifting of ethanol production.
Absorbance according to measured by periodic sampling, brings curve equation into and calculates ethanol in 1mL stalk fermentation liquid
Amount, take the 1mL of zymotic fluid to account for the 1/10 of measured quantity, sample liquid actually diluted 10 times, therefore result is multiplied by 10 and drawn
The yield mg/mL of final ethanol, then g/L is translated into, with regard to the alcohol output of 1g stalks and the yield of alcohol can be calculated.
Simultaneous saccharification and fermentation maximum conversion rate in this experiment is 0.1804g/g (dry straw).
Following table be 1g maize straw simultaneous saccharification and fermentations 72h after, the alcohol output and yield of diastatic fermentation liquid.
The optimal yield of alcohol and optimal yield under the conditions of the different pre-treatments of table 4
In summary, it is small 72 though the content of ethanol is increased over time and increased in simultaneous saccharification and fermentation liquid
Peak value is constantly basically reached, the content of fermenting alcohol is further continued for also without obvious ascendant trend, admittedly judge that synchronous fermentation reacts
72 hours more suitable.To the maize straw of different-grain diameter size, the alcohol getting rate highest of 2.5cm particle diameters, is 0.1804g/g,
The alcohol getting rate of 0.5cm and 1.5cm particle diameters is respectively 0.1428g/g and 0.1562g/g：For different storage times, during storage
Between be the maize straw alcohol getting rate highest of 3 months, be 0.1750g/g, and storage time obtained for the ethanol of 6 months and 12 months
Rate is all 0.1616g/g；For different storage modes, the yield of ethanol is optimal when not crushing wet storage, up to 0.1589g/
G, it is slightly lower not crush dry storage, is 0.1428g/g, crush wet storage and crush dry storage be respectively 0.1267g/g and
0.1321g/g.That is be storage mode not crush wet storage, storage time in condition be 3 months, particle size be
The alcohol getting rate highest of maize straw simultaneous saccharification and fermentation under 2.5cm Pretreatment.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (1)
1. a kind of maize straw synchronous fermentation process, it is characterised in that comprise the following steps：
S1, collection also stalk of the water content without aging more than 50% were placed in cryogenic conditions sealing preserve after 3 months, took
Go out, be crushed to the corn stalk pelletses that particle diameter is 2.5cm；
S2, using 1.5%NaOH solution the corn stalk pelletses of gained are soaked, be subsequently placed in 80 DEG C of water bath with thermostatic control conditions
Lower reaction 1h carries out alkali formula extractive reaction；After completion of the reaction, products therefrom is washed to neutral pH with pure water, then dried
Processing；
S3, take brewer's dried yeast appropriate, dissolved in the ratio that OD values are 2, then bacterium solution is transferred to the potato configured
Cultivated on culture medium, after bacterium is covered with thereon, carry out tube purifying, continue cultivate, it is to be purified after saccharomyces cerevisiae just
Cover with after slant tube culture medium, it is standby；
S4, the maize straw obtained by step S2 is placed in the reaction system that initial substrate concentration is 1g/100mL, cellulase
Consumption is 30FPU/g, and beta-glucosidase enzyme dosage is 15FPU/g, and adding saccharomyces cerevisiae makes the OD values of yeast in system be 2, lemon
Lemon acid sodium regulation pH is 4.8, then under conditions of 36 DEG C, with 80rmp per minute speed sync diastatic fermentation 96h, fermentation
After the completion of, using supercentrifuge with 10000r/min centrifugation 5min, maize straw residue precipitation is discarded, must be hydrolyzed
Liquid；
S5, the in mass ratio 1% addition proper amount of active carbon in the hydrolyzate of gained, so as to complete the detoxification treatment of hydrolyzate.
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