CN107290417A - A kind of biology sensor for improving reagent crystal habit - Google Patents
A kind of biology sensor for improving reagent crystal habit Download PDFInfo
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- CN107290417A CN107290417A CN201710454400.3A CN201710454400A CN107290417A CN 107290417 A CN107290417 A CN 107290417A CN 201710454400 A CN201710454400 A CN 201710454400A CN 107290417 A CN107290417 A CN 107290417A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
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Abstract
The invention discloses a kind of biology sensor for improving reagent crystal habit, include insulated substrate, electrode system, middle interlayer and coating successively from bottom to up;The electrode system at least includes working electrode and to electrode;The fixed detection reagent at least covering working electrode on the electrode system;The middle interlayer is in sample channel and reaction chamber region hollow out;The coating and middle interlayer and insulated substrate are built into reative cell and sample introduction capillary channel;The reative cell has air-vent;The composition of the detection reagent includes the chemical substance with amino.The present invention is added after the chemical substance with amino in detection reagent component, it effectively can eliminate or reduce " coffee ring " phenomenon after detection reagent solidification, make form of the detection reagent on electrode system it is more uniform, it is careful with it is consistent, be obviously improved biology sensor product test precision performance.Particularly using six ammonia ruthenium trichlorides as the detection reagent of electron mediator, effect highly significant of the invention.
Description
Technical field
The present invention relates to biological detection instrument field, and in particular to a kind of biology sensor of improvement reagent crystal habit.
Background technology
Biology sensor (biosensor) is a kind of sensitive to biological substance and its concentration is converted into electric signal progress
The instrument of detection.It is to make recognition component (including enzyme, antibody, antigen, microorganism, cell, group by the biological sensitive materials of immobilization
Knit, the bioactive substance such as nucleic acid), appropriate physics and chemistry transducer (such as oxygen electrode, photosensitive tube, FET, piezo-electric crystal
Deng) and signal amplifying apparatus constitute analysis tool or system.
Electrochemica biological sensor has been widely used in in-vitro diagnosis field, and these instrument systems can be detected in body fluid
Physiologically substance, facilitate doctor to analyze and diagnose patient status, also there are some instrument systems to be applied to family and use, convenient disease
The self-monitoring of people, these physical signs include blood glucose, cholesterol, uric acid, triglyceride, lactic acid, ketoboidies, enzyme etc..Mesh
Preceding biology sensor has been widely used in clinical detection, wherein most popular example is portable blood sugar test system.
Current biosensor testing plate is generally made up of following components:Insulated substrate, is used as the carrier of product;
The electrode system on insulated substrate is built, at least including working electrode and to electrode;It is fixed on the detection examination on electrode system
Agent;Opening at middle interlayer, reative cell and sample intake passage, is overlayed on electrode system;Coating, is covered on middle interlayer, and substrate,
Middle interlayer constitutes reative cell together.
The precision performance of the biology sensor product of Clinical practice is extremely important, the detection reagent wherein on working electrode
Whether crystal habit unanimously directly affects the precision performance of product.But, current detection reagent crystal habit is technically general
Store-through problem be " coffee ring " phenomenon, especially with six ammonium ruthenium trichlorides be electron mediator detection reagent, specific table
It is now that low or form is slightly uneven in graniphyric, particle between reagent solidifies back edge senior middle school, directly results in reagent on electrode
It is distributed became uneven, having various reactions causes, and is that product further lifts one of major obstacle of precision performance.
The content of the invention
To overcome the defect that above-mentioned prior art is present, the present invention is intended to provide a kind of detection reagent crystal habit it is uniform,
The good biology sensor of test precision, so as to solve problem-" coffee ring " phenomenon of prior art generally existing.
The purpose of the present invention is achieved through the following technical solutions:A kind of bio-sensing for improving reagent crystal habit
Device, available for specific substance concentration or activity in detection sample, its composition is included:Insulated substrate;Build on insulated substrate
Electrode system, described electrode system at least includes working electrode and to electrode;It is fixed on electrode system and should at least covers
The detection reagent of lid working electrode;The middle interlayer above electrode system is built, described middle interlayer is in sample channel and reaction
Chamber region hollow out;The coating above interlayer is located at, described coating and middle interlayer and insulated substrate are built into reative cell
And sample introduction capillary channel, sample can pass through capillary siphonage auto injection;Described reative cell should have air-vent, to be formed
Siphonage;Wherein, the composition of described detection reagent includes the chemical substance with amino.
Insulated substrate is the carrier of biology sensor.In the present invention, insulated substrate is preferably soft with certain deflection
Property plastic sheet, the material such as including but not limited to PET, PP, PVC, acrylic.There are a variety of commercialized products to be available for choosing at present
Select, such as Dupont companiesST 504, ST 339 etc..
Electrode system is built on insulated substrate, at least including to electrode and working electrode.The material of electrode is included but not
It is limited to one kind in graphite, silver, gold, platinum, palladium, ITO, IZO, Laser Processing etc. can be combined by silk-screen printing, vacuum splashing and plating
Technique is built on insulated substrate.
Middle interlayer, builds above electrode system, reacts chamber region hollow out, reative cell i.e. sample introduction is formd with reference to coating
Passage.The thickness of described middle interlayer be 0.025mm~0.5mm, preferably, the thickness of middle interlayer be 0.075mm~
0.125mm.Middle interlayer material can, with base material or without the adhesive tape of base material, be bonded up after processing;Can also be glue or
Polymer paste, is got on by silk-screen printing;As separated on electrode without other insulating materials, the material of middle interlayer must be good
Insulating materials.
Coating, above middle interlayer, and middle interlayer, insulated substrate are built into reative cell and sample intake passage, will pass through
Siphonage auto injection.Coating can be used PET material, the material that the hydrophilic treated of preferably clear is crossed, transparent to help to use
Family easily confirms reaction zone sample introduction state, hydrophilic sample introduction to be made more smoothly, and non-reaction chamber region can print sample introduction with opaque
Mark and Logo, available commercialization material have Adhesives Research, Inc's92804th, Coveme is public
Take charge of 9971 etc. of Kemafoil HHNW, 3M companies.
Air-vent, reative cell should have air-vent, and to form siphonage, air-vent is entered by coating or insulated substrate
The punching of sample passage end edge region realizes that also can reserve blow vent in sample intake passage end edge region by middle interlayer realizes.
Detection reagent is one of biology sensor core technology.Detection reagent is fixed on above electrode system, should be at least
Cover working electrode.Detection reagent can be fixed to by technology processing such as a liquid, silk-screen printing, inkjet printing, crack extrusions
On electrode.Detection reagent includes the components such as enzyme, electron mediator, buffer solution, surfactant, polymer.The effect of enzyme is special
Property and detected material reaction;Electron mediator obtains electronics from the reaction of enzyme and detected material, and is diffused into release on electrode
Electronics, and signal is formed, the signal can be tested instrument capture;Buffer solution ensures a suitable reaction environment;Surface-active
Agent can optimize reaction condition, and promote sample to spread;Polymer can form grid structure, and immobilized enzyme to a certain extent.
The method is mainly characterized in that including the chemical substance with amino in detection reagent, the chemistry that the material has leads to
Formula is as follows:
R1, R2, R3 variable groups of above-mentioned chemical general formula include be not limited to H, alkyl, acyl group and alkyl derivative,
The derivative of acyl group, two or all three groups of R1, R2, R3 group can form ring-type.It is typical in the chemical general formula
Material includes amino acid (such as serine, threonine, proline, alanine, lysine, histidine, lysine), imidazoles and miaow
Zole derivatives.
Inventor's research is found, is added in detection reagent after the chemical substance with amino, effectively can be eliminated or reduce inspection
Test agent solidification after " coffee ring " phenomenon, make form of the detection reagent on electrode system it is more uniform, it is careful with it is consistent, show
Work improves biology sensor product test precision performance.Tried particularly using six ammonia ruthenium trichlorides as the detection of electron mediator
Agent, effect highly significant of the invention.
In the present invention, the chemical substance with amino detection reagent prepare solution in concentration can for 1mM~
500mM, preferably 10mM~100mM.
Compared with prior art, the present invention has advantages below:
The present invention is added after the chemical substance with amino in detection reagent component, effectively can eliminate or reduce detection examination
Agent solidification after " coffee ring " phenomenon, make form of the detection reagent on electrode system it is more uniform, it is careful with it is consistent, significantly carry
Biology sensor product test precision performance is risen.Particularly using six ammonia ruthenium trichlorides as the detection reagent of electron mediator, this
The effect of invention highly significant.
Experimental results show that reference examples same concentration response curve discreteness is larger, precision is not ideal;
And the same concentration response curve of the embodiment of the present invention is very consistent, the precision of biology sensor is significantly improved.
Brief description of the drawings
Figure 1A and Figure 1B are that the STRUCTURE DECOMPOSITION of the biology sensor of a modification of the present invention reagent crystal habit is shown respectively
It is intended to and structural integrity schematic diagram.
Fig. 2 is the electrode system structural representation of the biology sensor of a modification of the present invention reagent crystal habit.
Fig. 3 is the detection reagent crystal habit figure of reference examples 1.
Fig. 4 is the detection reagent crystal habit figure of the embodiment of the present invention 1.
Fig. 5 is the detection reagent crystal habit figure of the embodiment of the present invention 2.
Fig. 6 is the detection reagent crystal habit figure of the embodiment of the present invention 3.
Fig. 7 is the detection reagent crystal habit figure of the embodiment of the present invention 4.
Fig. 8 is the detection reagent crystal habit figure of the embodiment of the present invention 5.
Fig. 9 is the detection reagent crystal habit figure of the embodiment of the present invention 6.
The response curve figure of Figure 10 reference examples 1.
The response curve figure of Figure 11 embodiment of the present invention 1.
The response curve figure of Figure 12 embodiment of the present invention 2.
The response curve figure of Figure 13 embodiment of the present invention 3.
The response curve figure of Figure 14 embodiment of the present invention 4.
The response curve figure of Figure 15 embodiment of the present invention 5.
The response curve figure of Figure 16 embodiment of the present invention 6.
Embodiment
With reference to embodiment and accompanying drawing, present disclosure is further illustrated.It should be appreciated that the implementation of the present invention is simultaneously
The following examples are not limited to, any formal accommodation and/or change made to the present invention fall within guarantor of the present invention
Protect scope.
In the present invention, if not refering in particular to, all equipment and raw material etc. are commercially available or the industry is conventional.
Method in following embodiments, is the conventional method of this area unless otherwise instructed.
Biosensor testing plate in embodiment constitutes explanation:
Insulated substrate, one kind that material includes but is not limited in PET, PP, PVC, acrylic.
Electrode system, builds on insulated substrate.Electrode system includes a pair of electrodes, working electrode, second pair of electrode,
A pair of electrodes, working electrode, second pair of electrode distal end from injection port to reaction channel are arranged in order.The material of electrode include but
One kind in graphite, silver, gold, platinum, palladium, ITO, IZO is not limited to, can be combined and laser machined by silk-screen printing, vacuum splashing and plating
Built etc. technique on insulated substrate.
Detection reagent, is fixed on electrode system, at least covers working electrode.Detection reagent is first configured to solution or slurry
Material, is then fixed on electrode system by techniques such as specking, silk-screen printing, crack extrusion, inkjet printings.The present invention is with grape
Sugar detection reagent is used as embodiment.
Middle interlayer is built above electrode system, reacts chamber region hollow out, reative cell i.e. sample introduction is formd with reference to coating
Passage.The thickness of described middle interlayer be 0.025mm~0.5mm, preferably, the thickness of middle interlayer be 0.075mm~
0.125mm.The material of middle interlayer can, with base material or without the adhesive tape of base material, be bonded up after processing;Can also be glue
Or polymer paste, got on by silk-screen printing;As separated on electrode without other insulating materials, the material of middle interlayer must be good
Good insulating materials.
Coating, above middle interlayer, and middle interlayer, insulated substrate are built into reative cell i.e. sample intake passage, will pass through
Siphonage auto injection.Coating can be used PET material, the material that the hydrophilic treated of preferably clear is crossed, transparent to help to use
Family easily confirms reaction zone sample introduction state, hydrophilic sample introduction to be made more smoothly, and non-reaction chamber region can print sample introduction with opaque
Mark and Logo, available commercialization material have Adhesives Research, Inc's92804th, Coveme is public
Take charge of 9971 hydrophilic films of Kemafoil HHNW, 3M companies etc..
Reative cell should have air-vent, and to form siphonage, the air-vent of the embodiment of the present invention passes through coating sample introduction
The punching of passage end edge region is realized.
Reference examples 1
The structure of biology sensor is as shown in Figure 1A, Figure 1B and Fig. 2.Electrode system 200 is built on insulated substrate 100;Institute
The electrode system 200 stated includes three electrodes:A pair of electrodes 201, working electrode 202, second pair of electrode 203, electrode are used
Carbon electrode, by insulated substrate 100 silk-screen printing carbon slurry solidify to form;Fixed test reagent 300 above electrode system, inspection
Test agent at least covers working electrode, distributes detection reagent solution by point gum machine and making is dried to electrode;Middle interlayer 400 is adopted
Two-sided glue material is used, thickness selects 0.1mm, and middle interlayer cuts hollow out by laser engraving or mould and produces sample intake passage and anti-
Room perforate is answered, middle interlayer is fitted on electrode system;Coating 500 uses transparent hydrophilic sheet material, passes through laser engraving or mould
Cutting produces air-vent 501 based on the correspondence reative cell end, and coating 500 is fitted on middle interlayer, coating and in every
Layer and insulated substrate are built into reative cell and sample intake passage 401, can pass through siphonage auto injection;Then compressed using machine
Be made complete biosensor testing plate 600, one end of the middle perforate of interlayer 400 is injection port 601, electrode system 200 it is every
Electrical leads extend to form connection haptic element 602 to biosensor testing plate other end, connection haptic element end insertion detector
Device port, the connection haptic element meeting contact corresponding with port per strip electrode is connected.
Wherein detection reagent 300 is first configured to the aqueous solution, and its composition is as follows:100mM sodium phosphate buffers (pH is 7.0),
1.2% (w/v) methylcellulose (Sigma M6385), 0.5% (w/v) Macrogol 4000 (Sigma 95904), 0.5%
(w/v) Triton X-100 (Aladdin T109026), 1.86% (w/v) six ammino ruthenium trichloride (CMI 8015), 2000U/
ML glucose oxidases (BBI Solutions GO3A).The detection reagent aqueous solution is fixed to bio-sensing using point gum machine distribution
On the electrode system of device test piece, every biosensor testing plate distributes the 1.0uL detection reagent aqueous solution, is subsequently placed in 60 and takes the photograph
Drying in 10 minutes in family name's degree baking oven.
Embodiment 1
The biosensor structure of embodiment 1 is with reference examples 1, and simply detection reagent component is different.
Detection reagent aqueous solution composition is as follows:100mM sodium phosphate buffers (pH is 7.0), 1.2% (w/v) Methyl cellulose
Plain (Sigma M6385), 0.5% (w/v) Macrogol 4000 (Sigma 95904), 0.5% (w/v) Triton X-100
(Aladdin T109026), 50mM imidazoles (Aladdin reagent I108707), 1.86% (w/v) six ammino ruthenium trichloride (CMI
8015), 2000U/mL glucose oxidases (BBI Solutions GO3A).The detection reagent aqueous solution is solid using point gum machine distribution
Determine onto the electrode system of biosensor testing plate, every biosensor testing plate distributes the 1.0uL detection reagent aqueous solution,
It is subsequently placed in drying in 10 minutes in 60 degrees Celsius of baking ovens.
Embodiment 2
The biosensor structure of embodiment 2 is with reference examples 1, and simply detection reagent component is different.
Detection reagent aqueous solution composition is as follows:100mM sodium phosphate buffers (pH is 7.0), 1.2% (w/v) Methyl cellulose
Plain (Sigma M6385), 0.5% (w/v) Macrogol 4000 (Sigma 95904), 0.5% (w/v) Triton X-100
(Aladdin T109026), 50mM L-threonines (Aladdin reagent T108221), 1.86% (w/v) six ammino ruthenium trichloride
(CMI 8015), 2000U/mL glucose oxidases (BBI Solutions GO3A).The detection reagent aqueous solution uses point gum machine
On electrode system of the distribution fixed to biosensor testing plate, every biosensor testing plate distributes 1.0uL detection reagents
The aqueous solution, is subsequently placed in drying in 10 minutes in 60 degrees Celsius of baking ovens.
Embodiment 3
The biosensor structure of embodiment 3 is with reference examples 1, and simply detection reagent component is different.
Detection reagent aqueous solution composition is as follows:100mM sodium phosphate buffers (pH is 7.0), 1.2% (w/v) Methyl cellulose
Plain (Sigma M6385), 0.5% (w/v) Macrogol 4000 (Sigma 95904), 0.5% (w/v) Triton X-100
(Aladdin T109026), 50mM L-PROLINEs (Aladdin reagent P108709), 1.86% (w/v) six ammino ruthenium trichloride
(CMI 8015), 2000U/mL glucose oxidases (BBI Solutions GO3A).The detection reagent aqueous solution uses point gum machine
On electrode system of the distribution fixed to biosensor testing plate, every biosensor testing plate distributes 1.0uL detection reagents
The aqueous solution, is subsequently placed in drying in 10 minutes in 60 degrees Celsius of baking ovens.
Embodiment 4
The biosensor structure of embodiment 4 is with reference examples 1, and simply detection reagent component is different.
Detection reagent aqueous solution composition is as follows:100mM sodium phosphate buffers (pH is 7.0), 1.2% (w/v) Methyl cellulose
Plain (Sigma M6385), 0.5% (w/v) Macrogol 4000 (Sigma 95904), 0.5% (w/v) Triton X-100
(Aladdin T109026), 50mM Serines (Aladdin reagent S103483), 1.86% (w/v) six ammino ruthenium trichloride
(CMI 8015), 2000U/mL glucose oxidases (BBI Solutions GO3A).The detection reagent aqueous solution uses point gum machine
On electrode system of the distribution fixed to biosensor testing plate, every biosensor testing plate distributes 1.0uL detection reagents
The aqueous solution, is subsequently placed in drying in 10 minutes in 60 degrees Celsius of baking ovens.
Embodiment 5
The biosensor structure of embodiment 5 is with reference examples 1, simply detection reagent component difference
Detection reagent aqueous solution composition is as follows:100mM sodium phosphate buffers (pH is 7.0), 1.2% (w/v) Methyl cellulose
Plain (Sigma M6385), 0.5% (w/v) Macrogol 4000 (Sigma 95904), 0.5% (w/v) Triton X-100
(Aladdin T109026), 10mM Serines (Aladdin reagent S103483), 1.86% (w/v) six ammino ruthenium trichloride
(CMI 8015), 2000U/mL glucose oxidases (BBI Solutions GO3A).The detection reagent aqueous solution uses point gum machine
On electrode system of the distribution fixed to biosensor testing plate, every biosensor testing plate distributes 1.0uL detection reagents
The aqueous solution, is subsequently placed in drying in 10 minutes in 60 degrees Celsius of baking ovens.
Embodiment 6
The biosensor structure of embodiment 6 is with reference examples 1, simply detection reagent component difference
Detection reagent aqueous solution composition is as follows:100mM sodium phosphate buffers (pH is 7.0), 1.2% (w/v) Methyl cellulose
Plain (Sigma M6385), 0.5% (w/v) Macrogol 4000 (Sigma 95904), 0.5% (w/v) Triton X-100
(Aladdin T109026), 100mM Serines (Aladdin reagent S103483), 1.86% (w/v) six ammino ruthenium trichloride
(CMI 8015), 2000U/mL glucose oxidases (BBI Solutions GO3A).The detection reagent aqueous solution uses point gum machine
On electrode system of the distribution fixed to biosensor testing plate, every biosensor testing plate distributes 1.0uL detection reagents
The aqueous solution, is subsequently placed in drying in 10 minutes in 60 degrees Celsius of baking ovens.
Test case
Using the venous whole of anticoagulant heparin, it is deployed into through 7 interval 0~700mg/dL concentration of glucose levels
Sample, wherein low concentration sample can place glycolysis by blood sample and obtain, and high concentration sample can be by adding high concentration grape
Sugar juice is modulated, and the concentration of actual allotment is:0mg/dL、58.4mg/dL、115.7mg/dL、174.2mg/dL、291.5mg/
dL、432.6mg/dL、645.6mg/dL.By above-mentioned reference examples 1 and embodiment 1-6 7 kinds of biosensor testing plates in test
Test comparison is carried out on instrument, instrument working electrode and the potential difference to electrode are set to 0.30V, every kind of each concentration sample of test piece
Product test 5, and test result was using 5 seconds electric currents.
The test result of embodiment 1-6 and comparative example 1 is shown in Fig. 3~Figure 16 and such as following table 1-7.
Table 1:The test result of reference examples 1
Table 2:The test result of embodiment 1
Table 3:The test result of embodiment 2
Table 4:The test result of embodiment 3
Table 5:The test result of embodiment 4
Table 6:The test result of embodiment 5
Table 7:The test result of embodiment 6
From 1~table of table 7 as can be seen that embodiment 1-6 in addition to 0 concentration, coefficient of variation average ratio reference examples 1 are significantly reduced
(0 concentration factor value is too low, and the coefficient of variation is relative to change too big, and comparison is not included), the coefficient of variation average of reference examples 1 is 3.1%,
Embodiment coefficient of variation average is 1.0%~2.0%, it is seen that the test that the present invention can effectively improve biological sensor chip is accurate
Degree.
Fig. 3~Fig. 9 is reference examples 1 and embodiment 1-6 detection reagent crystal habit figure.It can be seen that " the coffee of reference examples 1
Circle " phenomenon is notable, detection reagent form very heterogeneity;And the detection reagent crystal habit of six embodiments is well, one by one
Cause, without notable " coffee ring " phenomenon.
Figure 10~Figure 16 is reference examples 1 and embodiment 1-6 7 concentration determination response curve figures.It can be seen that reference examples are same
Concentration-response curve discreteness is larger, and the precision for illustrating the biology sensor of reference examples is not ideal;And six implementations
The same concentration response curve of example is very consistent, illustrates that the precision of the biology sensor of the present invention is significantly improved.
Claims (6)
1. a kind of biology sensor for improving reagent crystal habit, includes successively from bottom to up:Insulated substrate, electrode system, in
Interlayer and coating;The electrode system at least includes working electrode and to electrode;Fixation is at least covered on the electrode system
The detection reagent of working electrode;The middle interlayer is in sample channel and reaction chamber region hollow out;The coating and middle interlayer and
Insulated substrate is built into reative cell and sample introduction capillary channel;The reative cell has air-vent;Characterized in that, the detection examination
The composition of agent includes the chemical substance with amino.
2. a kind of biology sensor for improving reagent crystal habit according to claim 1, it is characterised in that described tool
There is the chemical general formula of the chemical substance of amino as follows:
R1, R2, R3 variable groups of above-mentioned chemical general formula include derivative, the derivative of acyl group of H, alkyl, acyl group and alkyl
Thing.
3. a kind of biology sensor for improving reagent crystal habit according to claim 2, it is characterised in that described
Two or all three groups formation ring-type of R1, R2, R3 variable groups.
4. a kind of biology sensor for improving reagent crystal habit according to claim 2, it is characterised in that described tool
The chemical substance for having amino includes amino acid, imidazoles and imdazole derivatives.
5. a kind of biology sensor for improving reagent crystal habit according to claim 1, it is characterised in that described tool
It is 1mM~500mM to have concentration of the chemical substance of amino in detection reagent prepares solution.
6. according to claim 1 or 5 it is a kind of improve reagent crystal habit biology sensor, it is characterised in that it is described
The chemical substance with amino detection reagent prepare solution in concentration be 10mM~100mM.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108896635A (en) * | 2018-09-20 | 2018-11-27 | 江苏鱼跃医疗设备股份有限公司 | A kind of beta-hydroxybutyric acid electrochemical sensor |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20120138484A1 (en) * | 2010-12-03 | 2012-06-07 | Abbott Diabetes Care Inc. | Analyte Sensors Comprising Thickeners, Enzyme Stabilizers and Osmium Boronates |
CN106124595A (en) * | 2016-06-17 | 2016-11-16 | 浙江亿联健医疗器械有限公司 | A kind of biosensor and preparation method thereof and reaction reagent |
CN106226379A (en) * | 2016-07-10 | 2016-12-14 | 浙江亿联健医疗器械有限公司 | A kind of biosensor eliminating sample introduction process influence and method of testing |
-
2017
- 2017-06-15 CN CN201710454400.3A patent/CN107290417A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20120138484A1 (en) * | 2010-12-03 | 2012-06-07 | Abbott Diabetes Care Inc. | Analyte Sensors Comprising Thickeners, Enzyme Stabilizers and Osmium Boronates |
CN106124595A (en) * | 2016-06-17 | 2016-11-16 | 浙江亿联健医疗器械有限公司 | A kind of biosensor and preparation method thereof and reaction reagent |
CN106226379A (en) * | 2016-07-10 | 2016-12-14 | 浙江亿联健医疗器械有限公司 | A kind of biosensor eliminating sample introduction process influence and method of testing |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108896635A (en) * | 2018-09-20 | 2018-11-27 | 江苏鱼跃医疗设备股份有限公司 | A kind of beta-hydroxybutyric acid electrochemical sensor |
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Application publication date: 20171024 |