CN107279073B - Indoor breeding method for coconut leaf moths - Google Patents
Indoor breeding method for coconut leaf moths Download PDFInfo
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- CN107279073B CN107279073B CN201710711026.0A CN201710711026A CN107279073B CN 107279073 B CN107279073 B CN 107279073B CN 201710711026 A CN201710711026 A CN 201710711026A CN 107279073 B CN107279073 B CN 107279073B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses an indoor breeding method of coconut hyphantria cunea, which collects eggs by adopting double-layer gauze, is beneficial to the collection of the eggs, adopts live plants for breeding at the stage of low-age larvae, does not need to replace blades, can reduce the breeding workload and can improve the survival rate of the larvae; the transparent bags with holes are adopted for feeding the young larvae, the humidity in the bags is kept better, the leaf fresh-keeping time is longer, and the feeding workload is reduced.
Description
Technical Field
The invention relates to a method for breeding insects, in particular to a method for breeding coco webworms indoors.
Background
Cocos nucifera is lepidoptera, is an important leaf eating pest of palmaceae plants, and is supplemented into forest dangerous pests by the national forest administration in 2014.
The following two patents exist for the breeding of the coconut tissue moth:
application number 201410024567.2 discloses a coconut pseudoplusia punctata mass propagation method, which specifically comprises the following steps: feeding the coconut leaf moths in various insect states under the conditions of 25-27 ℃ and 75% +/-10% relative humidity and natural illumination. Pairing male and female of the newly hatched coconut moths, introducing the paired cocoanut moths into a glass tube with old coconut leaves, and providing 10% honey water for supplying nutrients; 1-2 days later, the coco moths lay eggs on the coconut leaves; picking out the leaves with eggs, and intensively putting the leaves into an insect breeding box, wherein 1000 eggs are placed in each box; the eggs can be hatched into larvae after 6 to 7 days, and the leaves are replaced every 4 days; pupa is formed after 50 days, pupa eclosion is formed into imagoes after 9-12 days, and the imagoes enter the breeding process of the next cycle. The method uses old leaves of the coconut tree to feed the larvae, is simple and practical to operate, can continuously, evenly and massively propagate the various instar insects of the coconut pseudoplusia xylostella, and provides a large number of insect sources for basic research and prevention and control of the coconut pseudoplusia xylostella.
Application number 201410397993.0 discloses a method for breeding a large number of test populations of a coconut moth, which comprises the steps of improving the existing breeding method of the coconut moth according to the mating and spawning characteristics of the coconut moth, putting 5 pairs of newly emerged unmated male and female worms into a cylindrical transparent container with the height of 30cm and the diameter of 25cm, putting 10 healthy coconut tree leaves with the length of 15cm into each container, simultaneously supplementing nutrition for the adult coconut moth by cotton balls dipped with 10% honey water, sealing the opening of the container by double-layer 200-mesh gauze, replacing the leaves 1 time every 3 days, and replacing the cotton balls 1 time every day; continuously feeding 1-3 instar larvae in a larva feeding box with length of 30cm and width of 20cm, wherein each box has 1000 larvae, and blades are replaced 1 time every 7 days; the larvae of 4-8 years old are replaced in insect-culturing boxes with length of 40cm and width of 30cm, 400 heads are placed in each box, and the leaves are replaced 1 time every 4 days. Firstly, the mating space is enlarged, the mating number of male and female insects is increased, and the female insects are stimulated to lay eggs; in addition, female egg laying places are increased, egg laying area is increased, and egg survival rate is improved; finally, the complicated procedures of collecting eggs in separate test tubes and breeding and replacing leaves of larvae are simplified, the workload is greatly reduced, and the experimental population of the cocoanut moths with high survival rate, simplified procedures and large amount of standardization can be really obtained.
According to the feeding method of the two patents, 1-3 instar larvae with 1000 heads per box need to be replaced with 1 new leaf at most every 7 days, 4-8 instar larvae with 200 heads per box need to be replaced with 1 leaf every 4 days, and experiments show that the collected leaves are in vitro, and even if the insects are not eaten in 4 days in the box, the leaves are withered, so that the feeding of the insects is influenced, particularly the low-age larvae.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide an indoor feeding method for the coconut pseudoplusia punctifera.
In order to achieve the purpose, the invention adopts the technical scheme that: an indoor breeding method of coconut pseudoplusia comprises the following steps:
(1) and feeding and spawning of the imago of the coconut leaf moth:
collecting 5 pairs of adult coconut moths in a transparent container, putting cotton balls dipped with honey water, putting Chinese fan palm leaves, sealing the container opening with a gauze, putting wet double-layer gauze outside the gauze, and covering a container cover; replenishing honey water every day, replacing cotton balls every 3 days, keeping the double-layer gauze moist, and allowing the coco moths to lay eggs on the double-layer gauze;
(2) and feeding of the low-age larvae:
when eggs on the double-layer gauze turn red and appear black spots, transferring the double-layer gauze with the eggs and the Chinese fan palm leaves to leaves of potted young gerbera sunflowers, placing 950-1050 eggs on each leaf, and keeping the double-layer gauze moist; after 1-year larvae hatch out, coating vaseline on petioles of the seedlings;
(3) and breeding of the old larva:
when most of larvae grow to 3 years old, cutting off whole leaves of sunflower leaves of the old, transferring the leaves into transparent bags with holes, putting fresh sunflower leaves into each bag for 350-450 heads, and sealing the transparent bags; changing leaves once a week, mixing fresh Chinese fan palm leaves with old leaves with insects, and picking out old leaves without insects and dead insects with mildew; when the larva pupates, the pupae feathers into imagoes in the bag, the imagoes are put into a transparent container in a matching way, and the imagoes lay eggs and enter the next generation for breeding and propagation.
In step (1), the adult can cross the gauze and lay eggs on moist double-deck gauze, adopts moist double-deck gauze to be used for collecting the worm egg, and moist double-deck gauze more is favorable to collecting the worm egg, and the adult also more is inclined to laying eggs on moist double-deck gauze, nevertheless has a small amount of egg grain to produce on the body of a bottle, gets to eat through placing the automatic leaf that climbs to of the egg grain hatching back on the body of a bottle of chinese fan palm blade messenger.
In the step (1), the transparent container can be a transparent glass container, and the shape can be round or square; the container cover can cover the container and prevent the adult insects from running out, and the container cover can cover the article which enables the air to enter the transparent container, so that the gauze can be moisturized for a longer time, and the humidifying times of the double-layer gauze are reduced. The cotton balls dipped with honey can provide nutrition for adults.
In the step (2), potted young gerbils of 1-3 years old and the like are adopted to feed the young gerbils, so that on one hand, the condition that the leaves are dry due to water shortage in vitro can be avoided, the condition that the leaves are not required to be replaced when the bodies of the gerbils grow to 3 years old or above can be basically met, and a large amount of labor for replacing new leaves is saved; on the other hand, live plant breeding can improve the survival rate of larvae.
In the step (2), when the eggs on the double-layer gauze turn red and black spots appear, the eggs are about to hatch, the gauze is kept moist, and proper conditions are provided for survival of larvae; 950-1050 eggs are placed on each leaf, so that the food requirement of 1-3 instars of the number of larvae can be met to the maximum extent on one sunflower leaf of the old people. Vaseline is coated on the petiole of the seedling to prevent the larva of the coconut moth from crawling away.
In the step (3), the larvae of 3 ages are transferred into the transparent bags with holes for feeding, so that the moisture preservation and fresh-keeping time of the leaves can be prolonged, 350-400 larvae per bag can be maintained for 1 week, the leaves are fresh green, and the feeding workload is greatly reduced. Putting fresh Chinese fan palm leaves, and preferably wrapping the old man palm leaves by using the fresh Chinese fan palm leaves, so that the time for larvae to climb from the old man palm leaves to the fresh Chinese fan palm leaves is shortened; the transparent bag can be a transparent sealing bag, and the holes on the transparent bag can be formed by pricking the holes with insect needles.
Preferably, the honey water contains 10% by weight of honey.
Preferably, in step (1), the length of the Chinese fan palm leaf is 10 cm.
Preferably, when the double-layer gauze with the eggs is transferred to leaves of potted young geriatric sunflowers, the side, with the eggs, of the double-layer gauze is attached to the leaves. The purpose is to facilitate the larva to climb onto the leaves and improve the survival rate of the larva.
Preferably, the transparent container is a cylindrical transparent glass container with a diameter of 8.5cm and a height of 12 cm.
Preferably, the feeding conditions of each insect state of the coconut pseudoplusia comprise: under the condition of natural illumination, the temperature is 25-27 ℃, and the relative humidity is 75% +/-10%.
Preferably, the geriatric sunflower potted plantlet has a plant height of 50-60 cm and at least one completely unfolded new leaf.
The invention has the beneficial effects that: the invention provides an indoor breeding method of codling moth, which adopts live plants to breed at the low-age larva stage without replacing leaves, can reduce the breeding workload and improve the survival rate of the larva; the transparent bags with holes are adopted for feeding the young larvae, the humidity in the bags is kept better, the leaf fresh-keeping time is longer, and the feeding workload is reduced.
Drawings
FIG. 1 is a cylindrical glass bottle for rearing adult coconut moth in example 1;
FIG. 2 is a double layer of gauze with Cocos nucifera pieces in example 1;
FIG. 3 shows that the double-layered gauze with egg masses of example 1 is placed on an Elder sunflower plant;
FIG. 4 is the state of the leaves after 16 days of hatching in example 1;
FIG. 5 shows the state in which the middle-aged and high-aged larvae were kept in the clear bag in example 1.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
Example 1
According to the embodiment of the indoor feeding method of the cocoanut woven moth, the feeding state of each stage is shown in the figures 1-5, and the method specifically comprises the following steps:
(1) and feeding and spawning of the imago of the coconut leaf moth:
collecting 5 pairs of adult coconut leaf moths in a cylindrical glass container with diameter of 8.5cm and height of 12cm, putting cotton balls dipped with 10 wt% honey water, putting a 10cm leaf of Chinese fan palm, sealing the container opening with gauze, putting wet double-layer gauze outside the gauze, and covering with a culture dish (see figure 1); honey water is supplemented every day, cotton balls are replaced every 3 days, the double-layer gauze is kept moist, the coco moths lay eggs on the double-layer gauze, most of the eggs are laid on the double-layer gauze at the opening part of the glass bottle (see figure 2), and a very small number of the eggs are laid on the bottle wall; after eggs on the bottle wall are hatched, larvae automatically climb to the leaf of the Chinese fan palm;
(2) and feeding of the low-age larvae:
when the eggs on the double-layer gauze are turned into light yellow and black spots appear at one end, the double-layer gauze with the eggs and the Chinese fan palm leaves with larvae are transferred to the leaves of the potted plantlets of the old man sunflower with a fully-unfolded new leaf, the leaves are attached to one surface of the double-layer gauze with the eggs, 1000 eggs are placed on each leaf, and water is added every day to keep the gauze moist (see figure 3); when 1 st larva is hatched, taking food from leaves, coating vaseline on petioles of the seedlings to prevent the larva of the coconut moth from climbing away, and recovering the gauze after all eggs on the gauze are hatched;
(3) and breeding of the old larva:
when the larvae grow to 3 years old, the old sunflower leaves are eaten almost (see figure 4), the old sunflower leaves are cut off and transferred to a transparent sealing bag with holes, 400 larvae are put into each bag, the old sunflower leaves are packaged by fresh Chinese fan palm leaves, the young sunflower leaves are fixed by rubber bands, and the transparent bag is sealed (see figure 5); changing leaves once a week, mixing fresh Chinese fan palm leaves with old leaves with insects, and picking out old leaves without insects and dead insects with mildew; when the larva pupates, the pupae feathers into imagoes in the bag, the imagoes are put into a transparent container in a matching way, and the imagoes lay eggs and enter the next generation for breeding and propagation.
The conditions for feeding each insect state of the coconut pseudoplusia comprise: under the condition of natural illumination, the temperature is 25-27 ℃, and the relative humidity is 75% +/-10%.
Example 2
Three groups of rearing test results using the indoor rearing method of the coconut tissue moth described in example 1 were recorded as shown in table 1.
TABLE 1 survival rate of various insect-state breeding of Cocos nucifera
As can be seen from Table 1, the breeding survival rate of each insect state is high, which indicates that the indoor breeding method for the coconut pseudoplusia xylostella can reduce the breeding workload and improve the survival rate of larvae.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (5)
1. An indoor raising method of coconut pseudoplusia punctata is characterized by comprising the following steps:
(1) and feeding and spawning of the imago of the coconut leaf moth: collecting 5 pairs of adult coconut moths in a transparent container, putting cotton balls dipped with honey water, putting Chinese fan palm leaves, sealing the container opening with a gauze, putting wet double-layer gauze outside the gauze, and covering a container cover; replenishing honey water every day, replacing cotton balls every 3 days, keeping the double-layer gauze moist, and allowing the coco moths to lay eggs on the double-layer gauze;
(2) and feeding of the low-age larvae: when eggs on the double-layer gauze turn red and appear black spots, transferring the double-layer gauze with the eggs and the Chinese fan palm leaves to leaves of potted young gerbera sunflowers, placing 950-1050 eggs on each leaf, and keeping the double-layer gauze moist; after 1-year larvae hatch out, coating vaseline on petioles of the seedlings; when the double-layer gauze with the eggs is transferred to the leaves of the potted young gerbils, the side, with the eggs, of the double-layer gauze is attached to the leaves;
(3) and breeding of the old larva: when most of the larvae grow to 3 years old, cutting off whole leaves of sunflower leaves of the old, transferring the leaves into transparent bags with holes, putting fresh sunflower leaves into each bag for 350-450 larvae, and sealing the transparent bags; changing leaves once a week, mixing fresh Chinese fan palm leaves with old leaves with insects, and picking out old leaves without insects and dead insects with mildew; when the larva pupates and emerges in the bag, putting the pupae and the bag into a transparent container in a matching way, allowing the larva to lay eggs, and feeding and breeding the larva in the next generation;
the geriatric sunflower potted plantlet is 50-60 cm in plant height and is provided with at least one completely unfolded new leaf.
2. The method for indoor rearing of coconut pseudoplutella according to claim 1, wherein the honey water contains 10% by weight of honey.
3. The method for indoor rearing of a coconut pseudoplusia according to claim 1, wherein in the step (1), the length of the leaf of the Chinese fan palm is 10 cm.
4. The method for indoor rearing of coconut pseudoplutella according to claim 1, wherein the transparent container is a cylindrical transparent glass container having a diameter of 8.5cm and a height of 12 cm.
5. The indoor rearing method of a coconut pseudoplusia according to claim 1, wherein the rearing conditions for each insect state of the coconut pseudoplusia are: under the condition of natural illumination, the temperature is 25-27 ℃, and the relative humidity is 75% +/-10%.
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Citations (3)
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| CN103004703A (en) * | 2012-12-21 | 2013-04-03 | 浙江农林大学 | Method for breeding gall-inducing harmful insects |
| CN104186430A (en) * | 2014-08-14 | 2014-12-10 | 中国热带农业科学院椰子研究所 | Method for feeding a large number of laboratory test populations of Opisina arenosella Walkers |
| CN106106375A (en) * | 2016-06-30 | 2016-11-16 | 云南省农业科学院农业环境资源研究所 | A kind of method of water planting Caulis et Folium Brassicae capitatae indoor mass rearing diamondback moth |
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| JP3075619B2 (en) * | 1991-11-12 | 2000-08-14 | 日本農産工業株式会社 | Artificial feed for insects |
| CN102524194B (en) * | 2012-02-26 | 2014-03-19 | 新疆农业大学 | Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests |
| CN204466640U (en) * | 2015-02-25 | 2015-07-15 | 中国热带农业科学院环境与植物保护研究所 | Moth rearing-box knitted by coconut |
| CN204579614U (en) * | 2015-04-16 | 2015-08-26 | 中国热带农业科学院环境与植物保护研究所 | The device that moth egg lures collection knitted by a kind of coconut |
| CN106962293B (en) * | 2017-04-05 | 2020-07-10 | 山西省农业科学院植物保护研究所 | Indoor breeding method for single-head plutella xylostella |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004703A (en) * | 2012-12-21 | 2013-04-03 | 浙江农林大学 | Method for breeding gall-inducing harmful insects |
| CN104186430A (en) * | 2014-08-14 | 2014-12-10 | 中国热带农业科学院椰子研究所 | Method for feeding a large number of laboratory test populations of Opisina arenosella Walkers |
| CN106106375A (en) * | 2016-06-30 | 2016-11-16 | 云南省农业科学院农业环境资源研究所 | A kind of method of water planting Caulis et Folium Brassicae capitatae indoor mass rearing diamondback moth |
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