CN107226534A - A kind of cleanser of efficient process sanitary sewage - Google Patents
A kind of cleanser of efficient process sanitary sewage Download PDFInfo
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- CN107226534A CN107226534A CN201710669636.9A CN201710669636A CN107226534A CN 107226534 A CN107226534 A CN 107226534A CN 201710669636 A CN201710669636 A CN 201710669636A CN 107226534 A CN107226534 A CN 107226534A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/58—Treatment of water, waste water, or sewage by removing specified dissolved compounds
- C02F1/62—Heavy metal compounds
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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Abstract
The present invention provides a kind of cleanser of efficient process sanitary sewage, and the cleanser of the efficient process sanitary sewage is from top to bottom followed successively by the first heavy metal wastewater thereby flocbed, the first fixation of microbe composite layer, the second heavy metal wastewater thereby flocbed, the second fixation of microbe composite layer；The preparing raw material of the first heavy metal wastewater thereby flocbed and the second heavy metal wastewater thereby flocbed includes hydroxymethyl cellulose, metal ion solution, plant extracts；The metal ion solution includes ferric chloride solution, manganese sulfate solution；The plant extracts includes green-tea extract, honeybee flower extract, carrot extract, grape seed extract, dandelion extract, purslane extract, rosemary, Ramulus et Folium Spiraeae Salicifoliae extract, willowherb extract, red crowberry extract, wild cherry certain kind of berries extract.
Description
Technical field
The present invention relates to sewage treatment area, in particular it relates to a kind of cleanser of efficient process sanitary sewage.
Background technology
In sewage disposal, traditional activated sludge process is a kind of quite varied processing method of application, and this method is main
It is the organic matter gone using the microorganism contained in activated sludge in water removal, while these microorganisms can also play de- well
The effect of nitrogen dephosphorization.But, sludge can easily cause loss, waste of resource with drainage flow in traditional activated sludge process.
Immobilized microorganism technique can fix the superiority bacteria spp of the predetermined substance that can degrade through filtering out, the application of the technology
Sewage disposal system selectivity can be made, tolerance enhancing, treatment effect is stable, and operational management is simple, and degradation efficiency is substantially better than
Tradition.
Process for immobilizing enzyme/microbe mainly includes investment, cross-linking method and absorption method.Absorption method generally relies on biology
Effect between body and carrier, is that still the method stationarity is loosely, easily de- for a kind of fixing means simple and easy to apply, mild condition
Fall.Cross-linking method is also known as no-load immobilization method, is a kind of immobilization technology without carrier, and this method passes through chemistry, physical means
Make the crosslinking that is attached to each other between biological cell.Investment principle is the gelatin polymer that biological cell is trapped in water-insoluble
In the network of hole, this method is simple to operate, and stationarity is good, is presently the most conventional process for fixation.
Heavy metal is big to human body toxic action, ecological environment is caused to seriously endanger, effective plant extracts component can
Effectively to chelate the metal ion in waste water, it is allowed to form the mesh that stable compound flocculation sediment reaches isolation of purified waste water
's.
Therefore, in view of the above-mentioned problems, the present invention provides a kind of cleanser of efficient process sanitary sewage, its safety and stability,
Flocculation ability is strong, can effectively chelated metal ions reach wastewater treatment effect and immobilized microorganism stability it is good,
There is the effect efficiently removed to pollutant.
The content of the invention
In order to solve the above problems, first aspect present invention provides a kind of cleanser of efficient process sanitary sewage, described
The cleanser of efficient process sanitary sewage is from top to bottom followed successively by the first heavy metal wastewater thereby flocbed, the first fixation of microbe and is combined
Material layer, the second heavy metal wastewater thereby flocbed, the second fixation of microbe composite layer；
It is fine that the preparing raw material of the first heavy metal wastewater thereby flocbed and the second heavy metal wastewater thereby flocbed includes methylol
Tie up element, metal ion solution, plant extracts；
The metal ion solution includes ferric chloride solution, manganese sulfate solution；
The plant extracts includes green-tea extract, honeybee flower extract, carrot extract, grape seed extract, Pu
Public English extract, purslane extract, rosemary, Ramulus et Folium Spiraeae Salicifoliae extract, willowherb extract, red crowberry are extracted
Thing, wild cherry certain kind of berries extract；
The preparing raw material of the first fixation of microbe composite layer and the second fixation of microbe composite layer includes
Host material, alginate cross-linked gel, microbial cells, Sulfonated carbon nanotube；
The preparing raw material of the host material includes PLGA, ferroso-ferric oxide, polyvinyl alcohol.
In one embodiment, the preparation of the first heavy metal wastewater thereby flocbed and the second heavy metal wastewater thereby flocbed
The weight ratio of hydroxymethyl cellulose described in raw material and the plant extracts is 1：(0.2-0.6).
In one embodiment, the preparation of the first heavy metal wastewater thereby flocbed and the second heavy metal wastewater thereby flocbed
The weight ratio of hydroxymethyl cellulose described in raw material and the metal ion solution is 1：(0.8-1.2).
In one embodiment, the concentration of the ferric chloride solution is 0.3mol/L, the concentration of the manganese sulfate solution
For 0.13mol/L.
In one embodiment, the weight ratio of the ferric chloride solution and the manganese sulfate solution is 1：(0.3-0.5).
In one embodiment, calculate by weight, the plant extracts includes 5-10 parts of green-tea extracts, 1-5
Part honeybee flower extract, 2-4 parts of carrot extracts, 3-6 parts of grape seed extracts, 1-5 parts of dandelion extracts, 1-3 parts of dents
Amaranth extract, 2-5 part rosemary, 3-5 portions of Ramulus et Folium Spiraeae Salicifoliae extracts, 1-4 portions of willowherb extracts, the 3-7 portions of wild cherry certain kind of berries
Extract.
In one embodiment, calculate by weight, the plant extracts includes 8 portions of green-tea extracts, 4 portions of honeybees
Flower extract, 3 parts of carrot extracts, 4 parts of grape seed extracts, 3 parts of dandelion extracts, 2 parts of purslane extracts, 4 parts of fans
Repeatedly fragrant plant extracts, 4 parts of Ramulus et Folium Spiraeae Salicifoliae extracts, 2 parts of willowherb extracts, 5 parts of wild cherry certain kind of berries extracts.
In one embodiment, the first fixation of microbe composite layer and the second fixation of microbe composite
Microbial cells described in the preparing raw material of layer rely including trivalent arsenic oxidizing bacteria, Burkholderia group, spindle
It is one or more in propylhomoserin bacillus, bacillus pumilus group, pseudomonas.
In one embodiment, the first fixation of microbe composite layer and the second fixation of microbe composite
Alginate cross-linked gel described in the preparing raw material of layer is formed by the aqueous solution crosslinking of sodium alginate and calcium salt.
Another aspect of the present invention provides a kind of preparation method of the cleanser of efficient process sanitary sewage, including following step
Suddenly：
By the first heavy metal wastewater thereby flocbed, the first fixation of microbe composite layer, the second heavy metal wastewater thereby flocbed,
Second fixation of microbe composite layer is poured into mould in sequence, obtains the cleanser of the efficient process sanitary sewage.
The above-mentioned of the application and other features, aspect and advantage is more readily understood with reference to described further below.
Embodiment
The participation in the election detailed description of the invention below for being preferable to carry out method and including embodiment this hair can be more easily understood
Bright content.Unless otherwise defined, all technologies used herein and scientific terminology have common with art of the present invention
The identical implication that technical staff is generally understood that.When there is contradiction, the definition in this specification is defined.
As used herein term " by ... prepare " it is synonymous with "comprising".Term "comprising" used herein, " comprising ",
" having ", " containing " or its any other deformation, it is intended that cover including for non-exclusionism.For example, the combination comprising listed elements
Thing, step, method, product or device are not necessarily limited to those key elements, but can include not expressly listed other key elements or
Such a composition, step, method, product or the intrinsic key element of device.
Conjunction " Consists of " excludes any key element do not pointed out, step or component.If be used in claim, this
Phrase will make claim be closed, it is not included the material in addition to materials of those descriptions, but relative normal
Except rule impurity.When being rather than immediately following in the clause that phrase " Consists of " appears in claim main body after theme,
It is only limited to the key element described in the clause；Other key elements are not excluded outside as the overall claim.
Equivalent, concentration or other values or parameter are excellent with scope, preferred scope or a series of upper limit preferred values and lower limit
During the Range Representation that choosing value is limited, this, which is appreciated that, specifically discloses by any range limit or preferred value and any scope
All scopes that any pairing of lower limit or preferred value is formed, regardless of whether whether the scope separately discloses.For example, when open
During scope " 1 to 5 ", described scope should be interpreted as including scope " 1 to 4 ", " 1 to 3 ", " 1 to 2 ", " 1 to 2 and 4 to
5 ", " 1 to 3 and 5 " etc..When number range is described herein, unless otherwise indicated, otherwise the scope is intended to include its end
Value and all integers and fraction within the range.
Singulative includes plural number and object is discussed, unless the context clearly dictates otherwise." optional " or it is " any
It is a kind of " refer to that the item or event that describe thereafter may or may not occur, and the description include situation that event occurs and
The situation that event does not occur.
Approximate term in specification and claims is used for modifying quantity, represents that the present invention is not limited to this specific
Quantity, includes the part of the amendment of the acceptable change without cause related basic function close to the quantity.Phase
Answer, modify a numerical value with " about ", " about " etc., mean that the invention is not restricted to the exact numerical.In some examples, approximately
Term likely corresponds to the precision of the instrument of measured value.In present specification and claims, scope is limited can be with
Combine and/or exchange, these scopes include all subranges contained therebetween if not stated otherwise.
In addition, the indefinite article " one kind " and " one " before key element of the present invention or component are to key element or the quantitative requirement of component
(i.e. occurrence number) unrestriction.Therefore " one " or " one kind " should be read as including one or at least one, and odd number
The key element or component of form also include plural form, unless the obvious purport of the quantity refers to singulative.
" polymer " means by the polymerizable compound prepared by the monomer that polymerize identical or different type.Generic term
" polymer " includes term " homopolymer ", " copolymer ", " terpolymer " and " EVA ".
" EVA " means by polymerizeing polymer prepared by least two different monomers.Generic term " EVA " includes
(its is general with term " terpolymer " for term " copolymer " (it is typically to refer to the polymer prepared by two kinds of different monomers)
To refer to the polymer prepared by three kinds of different monomers).It also includes the polymer by polymerizeing more kinds of monomers and manufacturing.
" blend " means that two or more polymer passes through polymer formed by physics or chemistry the common mixing of method.
First aspect present invention provides a kind of cleanser of efficient process sanitary sewage, the efficient process sanitary sewage
Cleanser is from top to bottom followed successively by the first heavy metal wastewater thereby flocbed, the first fixation of microbe composite layer, the second heavy metal
Waste water flocculating layer, the second fixation of microbe composite layer；
It is fine that the preparing raw material of the first heavy metal wastewater thereby flocbed and the second heavy metal wastewater thereby flocbed includes methylol
Tie up element, metal ion solution, plant extracts；
The metal ion solution includes ferric chloride solution, manganese sulfate solution；
The plant extracts includes green-tea extract, honeybee flower extract, carrot extract, grape seed extract, Pu
Public English extract, purslane extract, rosemary, Ramulus et Folium Spiraeae Salicifoliae extract, willowherb extract, red crowberry are extracted
Thing, wild cherry certain kind of berries extract；
The preparing raw material of the first fixation of microbe composite layer and the second fixation of microbe composite layer includes
Host material, alginate cross-linked gel, microbial cells, Sulfonated carbon nanotube；
The preparing raw material of the host material includes PLGA, ferroso-ferric oxide, polyvinyl alcohol.
The preparation method of the Sulfonated carbon nanotube comprises the following steps：
(1) it is acidified CNT：The concentrated sulfuric acid and sodium nitrate of mass concentration 98%, frozen water are added in dry reactor
Bathe under the conditions of lower cooling, 0-5 DEG C, stirring adds CNT, be slowly added to potassium permanganate after being well mixed, controlling reaction temperature
For 10-15 DEG C, continuation stirring reaction 2h under the conditions of 2h, 35 DEG C is reacted, deionized water is added, reacting liquid temperature is controlled at 98 DEG C,
Continue to stir 0.5h, add the hydrogen peroxide that mass concentration is 30%, filter while hot, and product is entered with watery hydrochloric acid (1mol/L)
Row washing is dried under reduced pressure 24h to neutrality under the conditions of 60 DEG C, that is, obtains being acidified CNT；It is described acidifying CNT with it is described
Sodium nitrate, the weight ratio of the potassium permanganate are 1：0.6：3.4；The CNT and the dense sulphur of the mass concentration 98%
Hydrogen peroxide that sour, described mass concentration is 30%, the mass volume ratio of the deionized water are 1：18：3：20；
(2) sodium borohydride reduction acidifying CNT：The acidifying CNT that step (1) is obtained is added to deionized water
In, ultrasonically treated 1h, the acidifying carbon nano tube suspension being evenly distributed, 5wt% aqueous sodium carbonate regulation acidifying carbon
Nanotube suspension pH value reacts 1h under the conditions of 4% sodium borohydride aqueous solution, 80 DEG C are added to 9-10, then, is cooled to room
Temperature；The acidifying CNT is 1 with the sodium borohydride weight ratio：8；
(3) sodium nitrite in aqueous solution that mass fraction is 2% is added into reactor, is added under the conditions of 0-5 DEG C to ammonia
Base benzene sulfonic acid, HCl/water solution (1mol/L), react 24h, obtain aryl diazonium salts；The natrium nitrosum and the p-aminophenyl
Sulfonic acid, the weight ratio of the aqueous hydrochloric acid solution are 1：2.4：25；
(4) Sulfonated carbon nanotube：The aryl diazonium salts that step (3) is obtained slowly be added drop-wise to that step (2) obtains also
In former carbon nano tube suspension, and under the conditions of 0-5 DEG C and at room temperature each reaction 2h, it is saturating with deionized water after the completion of reaction
Analysis 10 days, is then sonicated 0.5h, filters, 80 DEG C are dried under reduced pressure 20h, obtain Sulfonated carbon nanotube.
Heretofore described PVA is polyvinyl alcohol；The PLGA is Poly(D,L-lactide-co-glycolide.
In the present invention, the preparation method of the host material comprises the following steps：
(1) PLGA/ ferroso-ferric oxide mixed solutions are prepared：PLGA, tetrahydrofuran are added into reactor, stirring has been treated
After fully dissolved, ferroso-ferric oxide is added, 0.5h is stirred；The PLGA and the tetrahydrofuran, the weight of the ferroso-ferric oxide
Than for 1：5：0.3；
(2) the PLGA/ ferroso-ferric oxides mixing obtained into PVA/ tetrahydrofuran mixed solution and dripping steps (1) is molten
After liquid, completion of dropping, after stirring 1h, 50 DEG C are removed under reduced pressure tetrahydrofuran, obtain loading the PLGA nanoparticles of ferroso-ferric oxide；
The weight ratio of the PVA and the ferroso-ferric oxide is 1：0.12；PVA and institute described in the PVA/ tetrahydrofurans mixed solution
The weight ratio for stating tetrahydrofuran is 1：3.
The preparation method of fixation of microbe composite in the fixation of microbe composite layer comprises the following steps：
(1) host material, Sulfonated carbon nanotube, sodium alginate, deionized water are mixed, is warming up to 85 DEG C, stir 2h, it is quiet
Put and be cooled to 40 DEG C, add microbial cells, mix 1h, obtain the mixed solution of to be fixedization microorganism；The matrix
Material and the Sulfonated carbon nanotube, the sodium alginate, the weight ratio of the deionized water are 1：0.01：0.012：20；Institute
The weight ratio for stating host material and the microbial cells is 1：0.02；
(2) calcium chloride is added in deionized water, stirred, is added dropwise into calcium chloride solution to be fixed in step (1)
Change the mixed solution of microorganism, be allowed to form spheric granules, stand, soak 20h, spheric granules is filtered and taken out, deionized water
Rinse, produce immobilization microorganism particles；The calcium chloride and the weight ratio of the deionized water are 0.02：1, the calcium chloride
Weight ratio with the host material is 0.03：1.
In one embodiment, ferroso-ferric oxide is the oxygen of magnetic nano-particle four in the preparing raw material of the host material
Change three-iron.
The preparation method of the magnetic nano-particle ferroso-ferric oxide is as follows：
Ferric acetyl acetonade, oleic acid, the dihydric alcohols of 1,2- ten, oleyl amine, benzyl ether are added into reactor, and is passed through nitrogen,
0.5h is stirred, 200 DEG C are warming up to, then insulation reaction 2h stops being passed through nitrogen, is warming up to 300 DEG C, insulation reaction 1h stops
Reaction, is cooled to room temperature, adds absolute ethyl alcohol, stirs 0.5h, filters, 60 DEG C are dried under reduced pressure 20h, obtain the magnetic nano particle
Sub- ferroso-ferric oxide；The ferric acetyl acetonade, the oleic acid, the dihydric alcohols of 1, the 2- ten, the oleyl amine, the benzyl ether,
The weight ratio of the absolute ethyl alcohol is 1：2.5：3：2.2：12：20.
In one embodiment, the preparation of the first heavy metal wastewater thereby flocbed and the second heavy metal wastewater thereby flocbed
The weight ratio of hydroxymethyl cellulose described in raw material and the plant extracts is 1：(0.2-0.6)；Preferably, the methylol
The weight ratio of cellulose and the plant extracts is 1：0.36.
In one embodiment, the preparation of the first heavy metal wastewater thereby flocbed and the second heavy metal wastewater thereby flocbed
The weight ratio of hydroxymethyl cellulose described in raw material and the metal ion solution is 1：(0.8-1.2)；Preferably, the hydroxyl first
The weight ratio of base cellulose and the metal ion solution is 1：0.9.
In one embodiment, the concentration of the ferric chloride solution is 0.3mol/L, the concentration of the manganese sulfate solution
For 0.13mol/L.
In one embodiment, the weight ratio of the ferric chloride solution and the manganese sulfate solution is 1：(0.3-0.5)；
Preferably, the weight ratio of the ferric chloride solution and the manganese sulfate solution is 1：0.42.
In one embodiment, calculate by weight, the plant extracts includes 5-10 parts of green-tea extracts, 1-5
Part honeybee flower extract, 2-4 parts of carrot extracts, 3-6 parts of grape seed extracts, 1-5 parts of dandelion extracts, 1-3 parts of dents
Amaranth extract, 2-5 part rosemary, 3-5 portions of Ramulus et Folium Spiraeae Salicifoliae extracts, 1-4 portions of willowherb extracts, the 3-7 portions of wild cherry certain kind of berries
Extract.
In one embodiment, calculate by weight, the plant extracts includes 8 portions of green-tea extracts, 4 portions of honeybees
Flower extract, 3 parts of carrot extracts, 4 parts of grape seed extracts, 3 parts of dandelion extracts, 2 parts of purslane extracts, 4 parts of fans
Repeatedly fragrant plant extracts, 4 parts of Ramulus et Folium Spiraeae Salicifoliae extracts, 2 parts of willowherb extracts, 5 parts of wild cherry certain kind of berries extracts.
Green-tea extract
Green tea is the not fermented tea being made, and remains the natural materials of fresh leaf, Tea Polyphenols, catechin, the Ye Lv contained
Element, caffeine, amino acid, vitamins and other nutritious components are also more.These natural nutrition compositions in green tea are to anti-aging, anti-
Cancer, anticancer, sterilization, anti-inflammatory etc. have special-effect.There is tealeaves the Main Ingredients and Appearance of pharmacological action to be Tea Polyphenols, caffeine, fat
Polysaccharide, theanine etc..
Extracting method：Load 2kg autumn and winter thick tea into steam distillation machine and infuse dust tea, thereto 35 DEG C of uniformly dispersing
After water 1kg, standing is allowed to moisten for 10 minutes.- the 54kPaG based on gauge pressure is decompressed to after the completion of moistening, then in SV (space velocity)
325h-1Lower carry out steam distillation, distillates 0.2kg distillate, obtains steam distillation liquid 1, then makes to return in containment system
Normal pressure is returned to, steam distillation is carried out at ambient pressure, 2kg distillate is distillated, obtained distillate is regard as steam distillation
Liquid 2, merges steam distillation liquid 1 and steam distillation liquid 2, is concentrated under reduced pressure into anhydrous, obtains green-tea extract.
Honeybee flower extract
Lemon balm (scientific name：Melissa axillaris (Benth.) Bakh.f.) perennial herb, have subterranean stem, on the ground
Stem is near upright or upright, branch, 4 ribs of tool and 4 shallow slots, high 0.6-1 meters, by pubescence.Leaf oval, it is long 1.2-6 centimetres, it is wide
0.9-3 centimetres, top is suddenly sharp or short tapering, and basal circular is blunt, near heart-shaped or anxious point, edge tool zigzag knuckle-tooth, herbaceous stem,
Blade face green, is dredged by pubescence, back side light green, by middle arteries both sides band purple or sometimes whole purples, near hairless or only along the pulse
By pubescence；Petiole is very thin.
Herb (axillary balm herb)：It is bitter, puckery, put down.It is clearing heat and detoxicating.For rheumatic numbness, leprosy is spitted blood, nosebleed epistaxis, dysentery, skin
Itch, sore rash, leprosy disease, uterine bleeding, snake bite.
Extracting method：3h is boiled in the decocting for adding 12 times of weight into dry lemon balm, decoction liquor is concentrated under reduced pressure, i.e.,
.
Carrot extract
Carrot alias daucus carrot, Hu Lu Fu, red reed Fu, cloves radish, Jin Sun, carrot, umbrella shape rib dish；Containing protein, fat
Fat, carbohydrate, carrotene, vitamin C and mineral matter, volatile oil etc.；Carrot is sweet, mild-natured, have strengthening the spleen and stomach, nourishing liver and improving eyesight,
The effects such as clearing heat and detoxicating, strengthening yang and invigorating kidney, promoting eruption, sending down abnormally ascending cough-relieving, available for digestive discomfort, constipation, the yctalopia (work of vitamin A
With), sexual disorder, measles, pertussis, the symptom such as infantile malnutrition.
Extracting method：Take dried carrot powder, be put into appropriate containers, add leaching liquor (ethyl acetate with it is anhydrous
Alcohol mixeding liquid by volume 2:1 is made), 1h dippings are heated at 80 DEG C, are then placed in micro-wave oven, microwave treatment is carried out, it is micro-
Ripple temperature sets 30 DEG C, stops after microwave treatment through supercentrifuge (3900rpm) centrifugation 30min, filtering concentrates filtrate, i.e.,
.
Grape seed extract
Grape pip contains abundant various amino acid, vitamin and mineral matter etc. because of it, and grape pip also contains choline：279mg/
Kg, hydrochloric acid：20mg/kg, pantothenic acid：34mg/kg, vitamin B1：0.17mg/kg, vitamin B2：2.5mg/kg.Grape pip is extracted
Thing OPC has superpower oxidation resistance, is 50 times of vitamin e, energy delaying aging, and prevention of arterial hardening also has skin to tie up him
The title of life, is 20 times of Vitamin C；There is grape seed extract removing free radical, anti-oxidant, anti-prostate cancer, anti-liver to swell
The effect of knurl, can also resist the damage of nervous system.
Extracting method：80% ethanol solution ultrasonic extraction 6h of 10 times of weight is added into dry grape pip, will be extracted
Liquid is concentrated under reduced pressure, and produces.
Dandelion extract
Dandelion alias dandelion, Po Poding, Fu Gongying, Pu Gongcao, sow with a drill straw in a mattress, public pod of falling forward, fall forward public small-mouthed jar, Chinese violet, gold
Wear in one's hair grass, comet comet fourth dish, chrysanthemum seedling, chrysanthemum youth, wood pigeon English, Bai Guding, Pu Gongding, ear scar grass, dog silkweed, milk it is careless, it is residual it is winged fall,
Yellow dog head, foretell that ground centipede, terrible lantern, goat milk milk is careless, double English foretell ground, sweet vernal grass, Gu Guding, fall forward public English, true phlegm it is careless；Efficacy effect：
It is clearing heat and detoxicating, dispersing swelling and dissipating binds, inducing diuresis for treating strangurtia.For furuncle swelling toxin, acute mastitis, scrofula, hot eyes, pharyngalgia, lung carbuncle, acute appendicitis, damp and hot Huang
Subcutaneous ulcer, puckery pain of heat gonorrhea.Dandelion contains abundant nutritive value, contains protein, fat, carbohydrate, trace element and dimension
Raw element etc..
Extracting method：4h is boiled in the decocting for adding 12 times of weight into dry dandelion plant, decoction liquor is depressurized dense
Contracting, is produced.
Purslane extract
The female dish of purslane alias dent dish, horse three-coloured amaranth, pig, edible kernel of melon seeds dish, melon seeds dish, longevity greens/mustard green, horse snake dish, HERBA PORTULACAE,
Horse amaranth, Wu Hangcao, purslane and hairyvein agrimonia, five sides grass, long life dish, peristrophe, sour amaranth, peaceful and comfortable dish, long life amaranth, sauce valve beans grass, snake
Grass, tart flavour dish, lion grass, horse dish, ant dish, horse step on dish, grey amaranth, sauce valve grass, beans plate dish, Ma Lai, resistance to Indian Rorippa Herb；Effect is made
With：It is clearing heat and detoxicating, cooling blood and hemostasis.For toxic-heat and blood stasis, carbuncle swells furunculosis, eczema, erysipelas, snake bite and insect sting is had blood in stool, hemorrhoid blood, uterine bleeding
Lower blood.Herb is containing a large amount of norepinephrines (noradrenaline) and volume sylvite (including potassium nitrate, potassium chloride, potassium sulfate
With other sylvite), also containing DOPA (dopa), dopamine (dopamine), betanidin (betanidin), isobetanidin
(isobetanidin), beet formula (betanin), different beet glucoside (isobetanin), oxalic acid (oxalic acid), malic acid
(malic acid), citric acid (citric acid), glutamic acid (glutamic acid), aspartic acid (aspartic
Acid), alanine (alanine) and glucose (glucose), fructose (fructose), sucrose (sucrose) etc..
Extracting method：4.5h is boiled in the decocting for adding 12 times of weight into the herb of dry purslane, and decoction liquor is depressurized
Concentration, is produced.
Rosemary
Rosemary Latin language scientific name：Rosmarinus officinalis, english name：Rosemary, alias：Ocean it
Dew；Rosemary system Labiatae Rosmarinus plant, is a kind of plant with high anti-oxidation effect generally acknowledged at present.In rosemary
Antioxidant content be mainly the compositions such as carnosic acid, carnosol, rosmanol, ursolic acid, Rosmarinic acid.Rosemary has
Aerogastria is eliminated, strengthens memory, awake angry, mitigation headache syndromes of refreshing oneself, improve the phenomenon of alopecia.
Extracting method：Pass through CO2Supercritical extraction is extracted from the cauline leaf of rosemary.
Ramulus et Folium Spiraeae Salicifoliae extract
The meadow sweet alias leech tip；Efficacy effect：It is clearing heat and detoxicating, for red eye, swell pain, have a headache, toothache, cough with lung heat；Outside
With controlling traumatic bleeding.Spiradine (Spiradine) A, B, C, D, F, G, spireine (Spireine) are isolated in herb
Deng alkaloid.
Extracting method：Pass through CO2Supercritical extraction is extracted from the herb of rosemary.
Willowherb extract
Willow herb is sturdy draft for many years, uprightly, is grown thickly；Root-like stock is crawled in topsoil extensively, up to 2 meters, slightly up to 2 lis
Rice, lignifying bears the strong shoot that survives the winter from basal part of stem.Willow herb herb bitter, it is nontoxic, there are detumescencing diuresis, lower breast, ease constipation
Function, cure mainly hypogalactia, deficiency of vital energy edema etc., root-like stock can be used as medicine, can anti-inflammatory analgetic, traumatic injury；It is main in willow herb herb
Contain 3- oxygen-galloyl-D-Glucose (3-O-galloyl-D-glucose, I), 1,6-, bis--oxygen-galloyl-β-
D- glucopyranoses (1,6-di-O-galloyl- β-D-glucopyra-nose, II), 1- oxygen-galloyl -4,6- six
Xenol formoxyl-β-D- glucopyranoses (1-O-galloyl-4,6-HHDP- β-D-glucopyra-nose, III),
Throatroot D prime (gemin D, IV), English oak (Quercus robur) ellagic acid (pedunculagin, V), trie Ma Su (tellimagrandin
I, VI), shrimp-roe florigen (woodfordin I, VII), chlorogenic acid (3-O-caffeoyl-quinic acid, VIII), gallic acid
(gallic acid, Ⅸ).
Extracting method：12 times of acetone/water solution ultrasonic extraction 6h of quality 70% are added into dry willow herb herb, will be extracted
Take liquid to be concentrated under reduced pressure, produce.
Wild cherry certain kind of berries extract
The wild cherry certain kind of berries is machaka, and the rose family originates in the wet woods of eastern North America and marsh；The wild cherry certain kind of berries contains abundant
Vitamin, mineral matter, polyphenoils and some other important polyphenols.The wild cherry certain kind of berries contain to mineral matter and Wei Sheng
Element, i.e.,：Vitamin B6、B12, E, C, folic acid, quininic acid, Polyphenol Acids, tannin, catechin, Mongolian oak flavine, phytomelin, aurantiamarin, in vain
Veratryl alcohol etc..
Extracting method：85% ethanol solution ultrasonic extraction 6h of 12 times of weight is added into the dry wild cherry certain kind of berries, will be extracted
Liquid is concentrated under reduced pressure, and produces.
In one embodiment, the material in the heavy metal wastewater thereby flocbed is heavy metal wastewater thereby flocculating composition,
Its preparing raw material includes hydroxymethyl cellulose, metal ion solution, plant extracts.
In the present invention, the preparation method of heavy metal wastewater thereby flocculating composition includes following in the heavy metal wastewater thereby flocbed
Step：
Into microwave reactor add hydroxymethyl cellulose, ferric chloride solution, the manganese sulfate solution, plant extracts,
Citric acid, deionized water, after stirring, are warming up to 90 DEG C, insulated and stirred 5min is cooled to room temperature, stand 2h, filtering is obtained
To heavy metal wastewater thereby flocculating composition；The weight ratio of the hydroxymethyl cellulose and the citric acid is 1：0.2.
In the present invention heavy metal wastewater thereby flocbed by induced chemical reduction mechanism, by stimulating organism reduction mechanism or
Person passes through chemistry and the combination purification sanitary sewage of biological reducing mechanism.Pass through the change of polyhydric phenols unique structure in plant extracts
Learn activity and physiological property, the characteristic being complexed with many kinds of metal ions, except the metal, a huge sum of money that may contain in decontaminated water
Category, arsenic compound and chromium compound；Heavy metal ion is combined with the group such as hydroxyl, carboxyl in plant extracts, forms covalent bond
Or ionic bond, this combination is quick；Heavy metal wastewater thereby flocculating composition can be used as reducing agent to destroy oxidant chemical combination
Thing such as perchlorate and nitrate, further improve the effect of purification sewage.
In one embodiment, the first fixation of microbe composite layer and the second fixation of microbe composite
Microbial cells described in the preparing raw material of layer rely including trivalent arsenic oxidizing bacteria, Burkholderia group, spindle
It is one or more in propylhomoserin bacillus, bacillus pumilus group, pseudomonas.
The preparation method of the microbial cells is as follows：
(1) BC soil of operational excellence and stable sanitary sewage disposal subsurface wastewater infiltration system is taken, is diluted with sterilized water,
Vibration dissolving, stands, and obtains bacteria suspension；
(2) strain isolation is carried out using dilution plate rubbing method, bacteria suspension is coated on ordinary solid culture medium, observed
Strain growth situation, carries out plate streaking purifying, to obtain many plants of different strains to single bacterium colony；The ordinary solid culture medium
Chemical composition and concentration are：Beef extract 5.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, 15.0~20.0g/L of agar, steam
Distilled water 1L/L；The cultivation temperature for setting immobilization bacterial strain is 30 DEG C.
In one embodiment, the first fixation of microbe composite layer and the second fixation of microbe composite
Alginate cross-linked gel described in the preparing raw material of layer is formed by the aqueous solution crosslinking of sodium alginate and calcium salt.
Another aspect of the present invention provides a kind of preparation method of the cleanser of efficient process sanitary sewage, including following step
Suddenly：
By the first heavy metal wastewater thereby flocbed, the first fixation of microbe composite layer, the second heavy metal wastewater thereby flocbed,
Second fixation of microbe composite layer is poured into mould in sequence, obtains the cleanser of the efficient process sanitary sewage.
The loose structure of mesostroma material and Sulfonated carbon nanotube of the present invention can increase the bond area of microorganism, through fixation
The microorganism concn of change is improved, it is ensured that the high concentration and high activity of microorganism；Host material and Sulfonated carbon nanotube
Loose structure is conducive to improving its mass-transfer performance, is conducive to oxygen, nutriment etc. to enter material internal for microorganism decomposition profit
With while being also beneficial to the metabolite discharge material outer of microorganism generation；In addition, containing polar group in Sulfonated carbon nanotube
Group sulfonic group functional group can be directly with microbial cell surface reactive group such as amino, hydroxyl etc. interconnected, formation be total to
Valence link further reaches the effect of solidification, further increases the concentration of microorganism, is conducive to improving microbe
Removal efficiency, strengthens sewage treating efficiency.Bacterial strain of the present invention is from operational excellence and stable sanitary sewage disposal underground
Filtration system, with high-efficient denitrification and dephosphorization function.
The present invention is specifically described below by embodiment.It is necessarily pointed out that, following examples are only used
In the invention will be further described, it is impossible to be interpreted as limiting the scope of the invention, professional and technical personnel in the field
Some the nonessential modifications and adaptations made according to the content of the invention described above, still fall within protection scope of the present invention.
In addition, if without other explanations, it is raw materials used be all it is commercially available, be purchased from traditional Chinese medicines chemical reagent.
Embodiment 1
The cleanser of the efficient process sanitary sewage be from top to bottom followed successively by the first heavy metal wastewater thereby flocbed, first consolidate
Determine microorganism composite layer, the second heavy metal wastewater thereby flocbed, the second fixation of microbe composite layer；First huge sum of money
Belonging to the preparing raw material of waste water flocculating layer and the second heavy metal wastewater thereby flocbed includes hydroxymethyl cellulose, metal ion solution, plant
Thing extract；The weight ratio of the hydroxymethyl cellulose and the plant extracts is 1：0.36；The hydroxymethyl cellulose with
The weight ratio of the metal ion solution is 1：0.9；The metal ion solution includes ferric chloride solution, manganese sulfate solution；Institute
The concentration for stating ferric chloride solution is that 0.3mol/L, the concentration of the manganese sulfate solution are 0.13mol/L；The ferric chloride solution with
The weight ratio of the manganese sulfate solution is 1：0.42；The plant extracts includes 8 parts of green-tea extracts, 4 portions of lemon balms and extracted
Thing, 3 parts of carrot extracts, 4 parts of grape seed extracts, 3 parts of dandelion extracts, 2 parts of purslane extracts, 4 parts of rosemary are planted
Thing extract, 4 parts of Ramulus et Folium Spiraeae Salicifoliae extracts, 2 parts of willowherb extracts, 5 parts of wild cherry certain kind of berries extracts；First fixation of microbe is combined
Material layer and the preparing raw material of the second fixation of microbe composite layer include host material, alginate cross-linked gel, micro- life
Thing thalline, Sulfonated carbon nanotube；The preparing raw material of the host material includes PLGA, ferroso-ferric oxide, polyvinyl alcohol；
The preparation method of the cleanser of the efficient process sanitary sewage, comprises the following steps：
By the first heavy metal wastewater thereby flocbed, the first fixation of microbe composite layer, the second heavy metal wastewater thereby flocbed,
Second fixation of microbe composite layer is poured into mould in sequence, obtains the cleanser of the efficient process sanitary sewage.
The preparation method of the host material comprises the following steps：
(1) PLGA/ ferroso-ferric oxide mixed solutions are prepared：PLGA, tetrahydrofuran are added into reactor, stirring has been treated
After fully dissolved, ferroso-ferric oxide is added, 0.5h is stirred；The PLGA and the tetrahydrofuran, the weight of the ferroso-ferric oxide
Than for 1：5：0.3；
(2) the PLGA/ ferroso-ferric oxides mixing obtained into PVA/ tetrahydrofuran mixed solution and dripping steps (1) is molten
After liquid, completion of dropping, after stirring 1h, 50 DEG C are removed under reduced pressure tetrahydrofuran, obtain loading the PLGA nanoparticles of ferroso-ferric oxide；
The weight ratio of the PVA and the ferroso-ferric oxide is 1：0.12；PVA and institute described in the PVA/ tetrahydrofurans mixed solution
The weight ratio for stating tetrahydrofuran is 1：3.
The preparation method of fixation of microbe composite in the fixation of microbe composite layer comprises the following steps：
(1) host material, Sulfonated carbon nanotube, sodium alginate, deionized water are mixed, is warming up to 85 DEG C, stir 2h, it is quiet
Put and be cooled to 40 DEG C, add microbial cells, mix 1h, obtain the mixed solution of to be fixedization microorganism；The matrix
Material and the Sulfonated carbon nanotube, the sodium alginate, the weight ratio of the deionized water are 1：0.01：0.012：20；Institute
The weight ratio for stating host material and the microbial cells is 1：0.02；
(2) calcium chloride is added in deionized water, stirred, is added dropwise into calcium chloride solution to be fixed in step (1)
Change the mixed solution of microorganism, be allowed to form spheric granules, stand, soak 20h, spheric granules is filtered and taken out, deionized water
Rinse, produce immobilization microorganism particles；The calcium chloride and the weight ratio of the deionized water are 0.02：1, the calcium chloride
Weight ratio with the host material is 0.03：1.
The preparation method of heavy metal wastewater thereby flocculating composition comprises the following steps in the heavy metal wastewater thereby flocbed：
Into microwave reactor add hydroxymethyl cellulose, ferric chloride solution, the manganese sulfate solution, plant extracts,
Citric acid, deionized water, after stirring, are warming up to 90 DEG C, insulated and stirred 5min is cooled to room temperature, stand 2h, filtering is obtained
To heavy metal wastewater thereby flocculating composition；The weight ratio of the hydroxymethyl cellulose and the citric acid is 1：0.2.
Embodiment 2
The cleanser of the efficient process sanitary sewage be from top to bottom followed successively by the first heavy metal wastewater thereby flocbed, first consolidate
Determine microorganism composite layer, the second heavy metal wastewater thereby flocbed, the second fixation of microbe composite layer；First huge sum of money
Belonging to the preparing raw material of waste water flocculating layer and the second heavy metal wastewater thereby flocbed includes hydroxymethyl cellulose, metal ion solution, plant
Thing extract；The weight ratio of the hydroxymethyl cellulose and the plant extracts is 1：0.2；The hydroxymethyl cellulose and institute
The weight ratio for stating metal ion solution is 1：0.9；The metal ion solution includes ferric chloride solution, manganese sulfate solution；It is described
The concentration of ferric chloride solution is that 0.3mol/L, the concentration of the manganese sulfate solution are 0.13mol/L；The ferric chloride solution and institute
The weight ratio for stating manganese sulfate solution is 1：0.42；The plant extracts include 8 parts of green-tea extracts, 4 parts of honeybee flower extracts,
3 parts of carrot extracts, 4 parts of grape seed extracts, 3 parts of dandelion extracts, 2 parts of purslane extracts, 4 parts of rosemary plants
Extract, 4 parts of Ramulus et Folium Spiraeae Salicifoliae extracts, 2 parts of willowherb extracts, 5 parts of wild cherry certain kind of berries extracts；The first fixation of microbe composite wood
The preparing raw material of the bed of material and the second fixation of microbe composite layer includes host material, alginate cross-linked gel, microorganism
Thalline, Sulfonated carbon nanotube；The preparing raw material of the host material includes PLGA, ferroso-ferric oxide, polyvinyl alcohol；
It is the preparation method of the cleanser of the efficient process sanitary sewage, the preparation method of the host material, described solid
Weighed in the preparation method and the heavy metal wastewater thereby flocbed of determining the fixation of microbe composite in microorganism composite layer
The preparation method be the same as Example 1 of metallic wastewater flocculating composition.
Embodiment 3
The cleanser of the efficient process sanitary sewage be from top to bottom followed successively by the first heavy metal wastewater thereby flocbed, first consolidate
Determine microorganism composite layer, the second heavy metal wastewater thereby flocbed, the second fixation of microbe composite layer；First huge sum of money
Belonging to the preparing raw material of waste water flocculating layer and the second heavy metal wastewater thereby flocbed includes hydroxymethyl cellulose, metal ion solution, plant
Thing extract；The weight ratio of the hydroxymethyl cellulose and the plant extracts is 1：0.6；The hydroxymethyl cellulose and institute
The weight ratio for stating metal ion solution is 1：0.9；The metal ion solution includes ferric chloride solution, manganese sulfate solution；It is described
The concentration of ferric chloride solution is that 0.3mol/L, the concentration of the manganese sulfate solution are 0.13mol/L；The ferric chloride solution and institute
The weight ratio for stating manganese sulfate solution is 1：0.42；The plant extracts include 8 parts of green-tea extracts, 4 parts of honeybee flower extracts,
3 parts of carrot extracts, 4 parts of grape seed extracts, 3 parts of dandelion extracts, 2 parts of purslane extracts, 4 parts of rosemary plants
Extract, 4 parts of Ramulus et Folium Spiraeae Salicifoliae extracts, 2 parts of willowherb extracts, 5 parts of wild cherry certain kind of berries extracts；The first fixation of microbe composite wood
The preparing raw material of the bed of material and the second fixation of microbe composite layer includes host material, alginate cross-linked gel, microorganism
Thalline, Sulfonated carbon nanotube；The preparing raw material of the host material includes PLGA, ferroso-ferric oxide, polyvinyl alcohol；
It is the preparation method of the cleanser of the efficient process sanitary sewage, the preparation method of the host material, described solid
Weighed in the preparation method and the heavy metal wastewater thereby flocbed of determining the fixation of microbe composite in microorganism composite layer
The preparation method be the same as Example 1 of metallic wastewater flocculating composition.
Embodiment 4
The cleanser of the efficient process sanitary sewage be from top to bottom followed successively by the first heavy metal wastewater thereby flocbed, first consolidate
Determine microorganism composite layer, the second heavy metal wastewater thereby flocbed, the second fixation of microbe composite layer；First huge sum of money
Belonging to the preparing raw material of waste water flocculating layer and the second heavy metal wastewater thereby flocbed includes hydroxymethyl cellulose, metal ion solution, plant
Thing extract；The weight ratio of the hydroxymethyl cellulose and the plant extracts is 1：0.36；The hydroxymethyl cellulose with
The weight ratio of the metal ion solution is 1：0.8；The metal ion solution includes ferric chloride solution, manganese sulfate solution；Institute
The concentration for stating ferric chloride solution is that 0.3mol/L, the concentration of the manganese sulfate solution are 0.13mol/L；The ferric chloride solution with
The weight ratio of the manganese sulfate solution is 1：0.42；The plant extracts includes 8 parts of green-tea extracts, 4 portions of lemon balms and extracted
Thing, 3 parts of carrot extracts, 4 parts of grape seed extracts, 3 parts of dandelion extracts, 2 parts of purslane extracts, 4 parts of rosemary are planted
Thing extract, 4 parts of Ramulus et Folium Spiraeae Salicifoliae extracts, 2 parts of willowherb extracts, 5 parts of wild cherry certain kind of berries extracts；First fixation of microbe is combined
Material layer and the preparing raw material of the second fixation of microbe composite layer include host material, alginate cross-linked gel, micro- life
Thing thalline, Sulfonated carbon nanotube；The preparing raw material of the host material includes PLGA, ferroso-ferric oxide, polyvinyl alcohol；
It is the preparation method of the cleanser of the efficient process sanitary sewage, the preparation method of the host material, described solid
Weighed in the preparation method and the heavy metal wastewater thereby flocbed of determining the fixation of microbe composite in microorganism composite layer
The preparation method be the same as Example 1 of metallic wastewater flocculating composition.
Embodiment 5
The cleanser of the efficient process sanitary sewage be from top to bottom followed successively by the first heavy metal wastewater thereby flocbed, first consolidate
Determine microorganism composite layer, the second heavy metal wastewater thereby flocbed, the second fixation of microbe composite layer；First huge sum of money
Belonging to the preparing raw material of waste water flocculating layer and the second heavy metal wastewater thereby flocbed includes hydroxymethyl cellulose, metal ion solution, plant
Thing extract；The weight ratio of the hydroxymethyl cellulose and the plant extracts is 1：0.36；The hydroxymethyl cellulose with
The weight ratio of the metal ion solution is 1：1.2；The metal ion solution includes ferric chloride solution, manganese sulfate solution；Institute
The concentration for stating ferric chloride solution is that 0.3mol/L, the concentration of the manganese sulfate solution are 0.13mol/L；The ferric chloride solution with
The weight ratio of the manganese sulfate solution is 1：0.42；The plant extracts includes 8 parts of green-tea extracts, 4 portions of lemon balms and extracted
Thing, 3 parts of carrot extracts, 4 parts of grape seed extracts, 3 parts of dandelion extracts, 2 parts of purslane extracts, 4 parts of rosemary are planted
Thing extract, 4 parts of Ramulus et Folium Spiraeae Salicifoliae extracts, 2 parts of willowherb extracts, 5 parts of wild cherry certain kind of berries extracts；First fixation of microbe is combined
Material layer and the preparing raw material of the second fixation of microbe composite layer include host material, alginate cross-linked gel, micro- life
Thing thalline, Sulfonated carbon nanotube；The preparing raw material of the host material includes PLGA, ferroso-ferric oxide, polyvinyl alcohol；
It is the preparation method of the cleanser of the efficient process sanitary sewage, the preparation method of the host material, described solid
Weighed in the preparation method and the heavy metal wastewater thereby flocbed of determining the fixation of microbe composite in microorganism composite layer
The preparation method be the same as Example 1 of metallic wastewater flocculating composition.
Embodiment 6
The cleanser of the efficient process sanitary sewage be from top to bottom followed successively by the first heavy metal wastewater thereby flocbed, first consolidate
Determine microorganism composite layer, the second heavy metal wastewater thereby flocbed, the second fixation of microbe composite layer；First huge sum of money
Belonging to the preparing raw material of waste water flocculating layer and the second heavy metal wastewater thereby flocbed includes hydroxymethyl cellulose, metal ion solution, plant
Thing extract；The weight ratio of the hydroxymethyl cellulose and the plant extracts is 1：0.36；The hydroxymethyl cellulose with
The weight ratio of the metal ion solution is 1：0.9；The metal ion solution includes ferric chloride solution, manganese sulfate solution；Institute
The concentration for stating ferric chloride solution is that 0.3mol/L, the concentration of the manganese sulfate solution are 0.13mol/L；The ferric chloride solution with
The weight ratio of the manganese sulfate solution is 1：0.42；The plant extracts is 40 parts of green-tea extracts；Described first fixes micro-
The preparing raw material of Biocomposite material layer and the second fixation of microbe composite layer includes host material, alginate crosslinking and coagulated
Glue, microbial cells, Sulfonated carbon nanotube；The preparing raw material of the host material includes PLGA, ferroso-ferric oxide, polyethylene
Alcohol；
It is the preparation method of the cleanser of the efficient process sanitary sewage, the preparation method of the host material, described solid
Weighed in the preparation method and the heavy metal wastewater thereby flocbed of determining the fixation of microbe composite in microorganism composite layer
The preparation method be the same as Example 1 of metallic wastewater flocculating composition.
Comparative example 1
The cleanser of the efficient process sanitary sewage be from top to bottom followed successively by the first heavy metal wastewater thereby flocbed, first consolidate
Determine microorganism composite layer；The preparing raw material of the first heavy metal wastewater thereby flocbed include hydroxymethyl cellulose, metal from
Sub- solution, plant extracts；The weight ratio of the hydroxymethyl cellulose and the plant extracts is 1：0.36；The methylol
The weight ratio of cellulose and the metal ion solution is 1：0.9；The metal ion solution includes ferric chloride solution, manganese sulfate
Solution；The concentration of the ferric chloride solution is that 0.3mol/L, the concentration of the manganese sulfate solution are 0.13mol/L；The chlorination
The weight ratio of ferrous solution and the manganese sulfate solution is 1：0.42；The plant extracts includes 8 parts of green-tea extracts, 4 parts of honey
Melissa extract, 3 parts of carrot extracts, 4 parts of grape seed extracts, 3 parts of dandelion extracts, 2 parts of purslane extracts, 4 parts
Rosemary, 4 parts of Ramulus et Folium Spiraeae Salicifoliae extracts, 2 parts of willowherb extracts, 5 parts of wild cherry certain kind of berries extracts；Described first fixes micro-
The preparing raw material of Biocomposite material layer includes host material, alginate cross-linked gel, microbial cells, sulfonation carbon nanometer
Pipe；The preparing raw material of the host material includes PLGA, ferroso-ferric oxide, polyvinyl alcohol；
Fixation of microbe composite in the preparation method of the host material, the fixation of microbe composite layer
Preparation method and the heavy metal wastewater thereby flocbed in heavy metal wastewater thereby flocculating composition preparation method be the same as Example 1.
The preparation method of the cleanser of the efficient process sanitary sewage is as follows：
First heavy metal wastewater thereby flocbed, the first fixation of microbe composite layer are poured into mould in sequence, obtained
To the cleanser of the efficient process sanitary sewage.
Comparative example 2
The cleanser of the efficient process sanitary sewage be from top to bottom followed successively by the first heavy metal wastewater thereby flocbed, first consolidate
Determine microorganism composite layer, the second heavy metal wastewater thereby flocbed, the second fixation of microbe composite layer, triple-heavy metal to give up
Water flocculation layer, the 3rd fixation of microbe composite layer；The first heavy metal wastewater thereby flocbed, the flocculation of the second heavy metal wastewater thereby
The preparing raw material of layer and triple-heavy metal waste water flocculating layer includes hydroxymethyl cellulose, metal ion solution, plant extracts；Institute
The weight ratio for stating hydroxymethyl cellulose and the plant extracts is 1：0.36；The hydroxymethyl cellulose and the metal ion
The weight ratio of solution is 1：0.9；The metal ion solution includes ferric chloride solution, manganese sulfate solution；The ferric chloride solution
Concentration be that 0.3mol/L, the concentration of the manganese sulfate solution are 0.13mol/L；The ferric chloride solution and the manganese sulfate are molten
The weight ratio of liquid is 1：0.42；The plant extracts is carried including 8 portions of green-tea extracts, 4 portions of honeybee flower extracts, 3 portions of carrots
Take thing, 4 parts of grape seed extracts, 3 parts of dandelion extracts, 2 parts of purslane extracts, 4 parts of rosemaries, 4 parts
Ramulus et Folium Spiraeae Salicifoliae extract, 2 parts of willowherb extracts, 5 parts of wild cherry certain kind of berries extracts；The first fixation of microbe composite layer, second
The preparing raw material of fixation of microbe composite layer and the 3rd fixation of microbe composite layer includes host material, alginate
Cross-linked gel, microbial cells, Sulfonated carbon nanotube；The preparing raw material of the host material includes PLGA, ferroso-ferric oxide, gathered
Vinyl alcohol；
Fixation of microbe composite in the preparation method of the host material, the fixation of microbe composite layer
Preparation method and the heavy metal wastewater thereby flocbed in heavy metal wastewater thereby flocculating composition preparation method be the same as Example 1.
The preparation method of the cleanser of the efficient process sanitary sewage is as follows：
By the first heavy metal wastewater thereby flocbed, the first fixation of microbe composite layer, the second heavy metal wastewater thereby flocbed,
Second fixation of microbe composite layer, triple-heavy metal waste water flocculating layer, the 3rd fixation of microbe composite layer are according to suitable
Sequence is poured into mould, obtains the cleanser of the efficient process sanitary sewage.
Comparative example 3
The cleanser of the efficient process sanitary sewage is from top to bottom followed successively by first the second weight of heavy metal wastewater thereby flocbed
Metallic wastewater flocbed；The preparing raw material of the first heavy metal wastewater thereby flocbed and the second heavy metal wastewater thereby flocbed includes hydroxyl
Methylcellulose, metal ion solution, plant extracts；The weight ratio of the hydroxymethyl cellulose and the plant extracts is
1：0.36；The weight ratio of the hydroxymethyl cellulose and the metal ion solution is 1：0.9；The metal ion solution includes
Ferric chloride solution, manganese sulfate solution；The concentration of the ferric chloride solution is that 0.3mol/L, the concentration of the manganese sulfate solution are
0.13mol/L；The weight ratio of the ferric chloride solution and the manganese sulfate solution is 1：0.42；The plant extracts includes 8
Part green-tea extract, 4 parts of honeybee flower extracts, 3 parts of carrot extracts, 4 parts of grape seed extracts, 3 parts of dandelion extracts, 2
Part purslane extract, 4 portions of rosemaries, 4 portions of Ramulus et Folium Spiraeae Salicifoliae extracts, 2 portions of willowherb extracts, 5 portions of wild cherry certain kind of berries are carried
Take thing；
The preparation method be the same as Example 1 of heavy metal wastewater thereby flocculating composition in the heavy metal wastewater thereby flocbed.
The preparation method of the cleanser of the efficient process sanitary sewage is as follows：
First heavy metal wastewater thereby flocbed, the second heavy metal wastewater thereby flocbed are poured into mould in sequence, institute is obtained
State the cleanser of efficient process sanitary sewage.
Comparative example 4
The cleanser of the efficient process sanitary sewage is from top to bottom followed successively by the first fixation of microbe composite layer,
Two fixation of microbe composite layers；The first fixation of microbe composite layer and the second fixation of microbe composite layer
Preparing raw material include host material, alginate cross-linked gel, microbial cells, Sulfonated carbon nanotube；The host material
Preparing raw material include PLGA, ferroso-ferric oxide, polyvinyl alcohol；
Fixation of microbe composite in the preparation method of the host material, the fixation of microbe composite layer
Preparation method be the same as Example 1.
The preparation method of the cleanser of the efficient process sanitary sewage is as follows：
First fixation of microbe composite layer, the second fixation of microbe composite layer are poured into mould in sequence
In, obtain the cleanser of the efficient process sanitary sewage.
Comparative example 5
The cleanser of the efficient process sanitary sewage be from top to bottom followed successively by the first heavy metal wastewater thereby flocbed, first consolidate
Determine microorganism composite layer, the second heavy metal wastewater thereby flocbed, the second fixation of microbe composite layer；First huge sum of money
Belonging to the preparing raw material of waste water flocculating layer and the second heavy metal wastewater thereby flocbed includes hydroxymethyl cellulose, metal ion solution, plant
Thing extract；The weight ratio of the hydroxymethyl cellulose and the plant extracts is 1：0.36；The hydroxymethyl cellulose with
The weight ratio of the metal ion solution is 1：0.9；The metal ion solution includes ferric chloride solution, manganese sulfate solution；Institute
The concentration for stating ferric chloride solution is that 0.3mol/L, the concentration of the manganese sulfate solution are 0.13mol/L；The ferric chloride solution with
The weight ratio of the manganese sulfate solution is 1：0.42；The plant extracts includes 8 parts of green-tea extracts, 4 portions of lemon balms and extracted
Thing, 3 parts of carrot extracts, 4 parts of grape seed extracts, 3 parts of dandelion extracts, 2 parts of purslane extracts, 4 parts of rosemary are planted
Thing extract, 4 parts of Ramulus et Folium Spiraeae Salicifoliae extracts, 2 parts of willowherb extracts, 5 parts of wild cherry certain kind of berries extracts；First fixation of microbe is combined
Material layer and the preparing raw material of the second fixation of microbe composite layer include host material, alginate cross-linked gel, micro- life
Thing thalline；The preparing raw material of the host material includes PLGA, ferroso-ferric oxide, polyvinyl alcohol；
It is the preparation method of the cleanser of the efficient process sanitary sewage, the preparation method of the host material, described solid
Weighed in the preparation method and the heavy metal wastewater thereby flocbed of determining the fixation of microbe composite in microorganism composite layer
The preparation method be the same as Example 1 of metallic wastewater flocculating composition, difference is consolidating in the fixation of microbe composite layer
Determine in microorganism composite without Sulfonated carbon nanotube.
Comparative example 6
The cleanser of the efficient process sanitary sewage be from top to bottom followed successively by the first heavy metal wastewater thereby flocbed, first consolidate
Determine microorganism composite layer, the second heavy metal wastewater thereby flocbed, the second fixation of microbe composite layer；First huge sum of money
Belonging to the preparing raw material of waste water flocculating layer and the second heavy metal wastewater thereby flocbed includes hydroxymethyl cellulose, metal ion solution, plant
Thing extract；The weight ratio of the hydroxymethyl cellulose and the plant extracts is 1：0.36；The hydroxymethyl cellulose with
The weight ratio of the metal ion solution is 1：0.9；The metal ion solution includes ferric chloride solution, manganese sulfate solution；Institute
The concentration for stating ferric chloride solution is that 0.3mol/L, the concentration of the manganese sulfate solution are 0.13mol/L；The ferric chloride solution with
The weight ratio of the manganese sulfate solution is 1：0.42；The plant extracts includes 8 parts of green-tea extracts, 4 portions of lemon balms and extracted
Thing, 3 parts of carrot extracts, 4 parts of grape seed extracts, 3 parts of dandelion extracts, 2 parts of purslane extracts, 4 parts of rosemary are planted
Thing extract, 4 parts of Ramulus et Folium Spiraeae Salicifoliae extracts, 2 parts of willowherb extracts, 5 parts of wild cherry certain kind of berries extracts；First fixation of microbe is combined
Material layer and the preparing raw material of the second fixation of microbe composite layer include Sulfonated carbon nanotube, alginate cross-linked gel,
Microbial cells；
Consolidating in the preparation method of the cleanser of the efficient process sanitary sewage, the fixation of microbe composite layer
Determine the preparation of heavy metal wastewater thereby flocculating composition in the preparation method and the heavy metal wastewater thereby flocbed of microorganism composite
Method be the same as Example 1, difference is in the fixation of microbe composite in the fixation of microbe composite layer without matrix
Material.
Performance test：
1st, the cleanser of efficient process sanitary sewage in the present invention is put into the simulated domestic wastewater of human configuration, to mould
Intend sanitary sewage to be handled, the cultivation temperature for setting immobilization bacterial strain is 30 DEG C, in t=48h, and immobilization bacterial strain is to simulation
COD, NH4 in sanitary sewage+- N and TP clearance；The chemical composition and concentration of the simulated domestic wastewater be：COD：
300mg/L, NH4+-N：50mg/L, TP：20mg/L；
2nd, the cleanser of efficient process sanitary sewage is added into the heavy metal aqueous solution through Tris (10mM) 2.4mM modulated
(CdCl2, NiCl2) in 100ml.Reaction is centrifuged after terminating, and the huge sum of money in the supernatant of separation is determined with atom extinction photometer
Belong to concentration.
The performance test results of table 1
It is seen from the above data that the cleanser for the efficient process sanitary sewage that the present invention is provided, its safety and stability, wadding
Solidifying ability is strong, can effectively chelated metal ions reach wastewater treatment effect and immobilized microorganism stability it is good, it is right
Pollutant has the effect efficiently removed.
Foregoing example is merely illustrative, some features of the feature for explaining the disclosure.Appended claim
The scope as wide as possible for requiring to be contemplated that is intended to, and embodiments as presented herein is only according to all possible embodiment
Combination selection embodiment explanation.Therefore, the purpose of applicant is appended claim not by the explanation present invention
Feature example selectional restriction.And the progress in science and technology by formed language performance it is inaccurate due to and not
The possible equivalent or son being presently considered are replaced, and these changes should also be interpreted by appended in the conceived case
Claim is covered.
Claims (10)
1. a kind of cleanser of efficient process sanitary sewage, it is characterised in that the cleanser of the efficient process sanitary sewage by
The first heavy metal wastewater thereby flocbed, the first fixation of microbe composite layer, the flocculation of the second heavy metal wastewater thereby are followed successively by under above
Layer, the second fixation of microbe composite layer；
The preparing raw material of the first heavy metal wastewater thereby flocbed and the second heavy metal wastewater thereby flocbed include hydroxymethyl cellulose,
Metal ion solution, plant extracts；
The metal ion solution includes ferric chloride solution, manganese sulfate solution；
The plant extracts includes green-tea extract, honeybee flower extract, carrot extract, grape seed extract, dandelion
Extract, purslane extract, rosemary, Ramulus et Folium Spiraeae Salicifoliae extract, willowherb extract, red crowberry extract, open country
Cherry certain kind of berries extract；
The preparing raw material of the first fixation of microbe composite layer and the second fixation of microbe composite layer includes matrix
Material, alginate cross-linked gel, microbial cells, Sulfonated carbon nanotube；
The preparing raw material of the host material includes PLGA, ferroso-ferric oxide, polyvinyl alcohol.
2. the cleanser of efficient process sanitary sewage according to claim 1, it is characterised in that first heavy metal wastewater thereby
The weight of hydroxymethyl cellulose described in the preparing raw material of flocbed and the second heavy metal wastewater thereby flocbed and the plant extracts
Amount is than being 1：(0.2-0.6).
3. the cleanser of efficient process sanitary sewage according to claim 1, it is characterised in that first heavy metal wastewater thereby
Hydroxymethyl cellulose described in the preparing raw material of flocbed and the second heavy metal wastewater thereby flocbed and the metal ion solution
Weight ratio is 1：(0.8-1.2).
4. the cleanser of efficient process sanitary sewage according to claim 1, it is characterised in that the ferric chloride solution it is dense
Degree is that 0.3mol/L, the concentration of the manganese sulfate solution are 0.13mol/L.
5. the cleanser of efficient process sanitary sewage according to claim 4, it is characterised in that the ferric chloride solution and institute
The weight ratio for stating manganese sulfate solution is 1：(0.3-0.5).
6. the cleanser of efficient process sanitary sewage according to claim 1, it is characterised in that calculate by weight, described
Plant extracts includes 5-10 parts of green-tea extracts, 1-5 parts of honeybee flower extracts, 2-4 parts of carrot extracts, 3-6 parts of grape pips
Extract, 1-5 part dandelion extract, 1-3 portions of purslane extracts, 2-5 portions of rosemaries, 3-5 portions of meadow sweets
Extract, 1-4 part willowherb extract, 3-7 parts of wild cherry certain kind of berries extracts.
7. the cleanser of efficient process sanitary sewage according to claim 6, it is characterised in that calculate by weight, described
Plant extracts includes 8 parts of green-tea extracts, 4 parts of honeybee flower extracts, 3 parts of carrot extracts, 4 parts of grape seed extracts, 3
Part dandelion extract, 2 portions of purslane extracts, 4 portions of rosemaries, 4 portions of Ramulus et Folium Spiraeae Salicifoliae extracts, 2 portions of willow herbs are carried
Take thing, 5 parts of wild cherry certain kind of berries extracts.
8. the cleanser of efficient process sanitary sewage according to claim 1, it is characterised in that first fixation of microbe
Microbial cells described in the preparing raw material of composite layer and the second fixation of microbe composite layer are aoxidized including trivalent arsenic
One in bacterium, Burkholderia group, spindle lysine bacillus, bacillus pumilus group, pseudomonas
Plant or a variety of.
9. the cleanser of efficient process sanitary sewage according to claim 1, it is characterised in that first fixation of microbe
Alginate cross-linked gel is by alginic acid described in the preparing raw material of composite layer and the second fixation of microbe composite layer
The aqueous solution crosslinking of sodium and calcium salt is formed.
10. the preparation method of the cleanser of efficient process sanitary sewage according to claim 1-9, it is characterised in that including
Following steps：
By the first heavy metal wastewater thereby flocbed, the first fixation of microbe composite layer, the second heavy metal wastewater thereby flocbed, second
Fixation of microbe composite layer is poured into mould in sequence, obtains the cleanser of the efficient process sanitary sewage.
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| CN107572612A (en) * | 2017-10-19 | 2018-01-12 | 王婧宁 | Glass-cutting acid sewage treating agent |
| CN107987847A (en) * | 2017-12-06 | 2018-05-04 | 吉林省中辰园林股份有限公司 | A kind of organic covering composition in environment-friendly type gardens |
| CN108383224A (en) * | 2018-04-12 | 2018-08-10 | 王建玲 | A kind of flocculant of environmentally protective degerming |
| CN108975476A (en) * | 2018-07-24 | 2018-12-11 | 芜湖市棠华建材科技有限公司 | The composite modified water treatment agent of plant |
| CN109095621A (en) * | 2018-08-03 | 2018-12-28 | 连云开 | A kind of application method of Aquacultural water water quality cleansing agent |
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| CN109095621A (en) * | 2018-08-03 | 2018-12-28 | 连云开 | A kind of application method of Aquacultural water water quality cleansing agent |
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Application publication date: 20171003 |