CN107192827A - A kind of anti-Miao Le pipes hormone（AMH）Detection means and method - Google Patents
A kind of anti-Miao Le pipes hormone（AMH）Detection means and method Download PDFInfo
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- CN107192827A CN107192827A CN201710568815.3A CN201710568815A CN107192827A CN 107192827 A CN107192827 A CN 107192827A CN 201710568815 A CN201710568815 A CN 201710568815A CN 107192827 A CN107192827 A CN 107192827A
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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Abstract
The present invention relates to a kind of anti-Miao Le pipes hormone (AMH) detection means and method, including multiple detection boxes, multiple reagent bottles, detection box is respectively used to sample detection, blank zeroing detection, contrasting detection, the contrast agent of various concentrations is respectively provided with reagent bottle, the detection box includes shell, sample pad, nitrocellulose filter, adsorptive pads, nitrocellulose filter is provided with the shell, one end connection sample pad of nitrocellulose filter, the other end connects adsorptive pads, on the shell sample well is provided with positioned at the position of sample pad, peep hole is provided with positioned at the position of nitrocellulose filter, the nitrocellulose filter is provided with detection line and quality inspection line.Convenient use of the present invention, can disposable detected, and ensure that testing result is accurate, is conducive to interpretation.
Description
Technical field
The present invention relates to medical instruments field, and in particular to a kind of anti-Miao Le pipes hormone (AMH) detection means and method.
Background technology
As modern female marriage and the postponement of childbearing age, and national child-bearing policy are constantly decontroled, elderly parturient women people
Number is substantially improved, and hard to settle, infertile crowd's proportion and Fertility reproductive risk are persistently increased.Investigation display, China's infertility in recent years
Generation it is in rising trend, average attack rate just has a pair of infertile, patients up to 12.5%~15% in that is, every 8 couples of men and wives
Sum has exceeded 5,0,000,000, wherein the most common with of the right age female ovulation dysfunction.The fecundity of women is internal each with it
Class reproductive hormone and Ovary reserve have close relationship.The fundamental prerequisite of female fertility is that have ovum, and ovarian follicle
Quantity determines the size of Ovary reserve with quality.The essence of fecundity decline, is exactly that Follicle number is reduced, ovum matter
Amount declines.So, we can be by detecting that Ovary reserve judges female fertility level.
Anti- antisterility, it is crucial to judge Ovary reserve.At present, clinically for helping doctor to judge women ovary
The reference index or method of reserve function are ultrasonic, in vitro fertilization including age, hormone test, ovary dynamic test, ovary
(IVF) index etc..Clinically use follotropin (FSH) and antral follicle count (AFC) weighs ovarian reserve follicle number
Amount.However, these indexs not only require there are many restrictions in detection time and operation, more because it can only be in primary ovarian work(
Measuring during the physiological performance phase occurs in energy deficiency, it is impossible to point out fecundity to decline in advance, causes the delay of diagnosis, misses and most preferably control
Treat the period.
The detection of anti-Miao Le pipes hormone (AMH) can efficiently solve these problems.AMH is a kind of by the small folliculus of ovary
Hormone secreted by Granulosa cells, can reflect the follicular activity of whole life cycle.When women is born, AMH in serum
Concentration level is very low, and AMH concentration peaks after puberty, and maintains high level in the whole childbearing age, afterwards with year
Age and various factors are gradually used up, and AMH concentration can be decreased, until post menopausal can not be measured.It can be seen that, AMH values can be with ovum
The change of nest function is in fluctuations.Also, relative to other indexs, AMH values are stablized relatively, can be any the one of the menstrual cycle
It is detected, is not influenceed by menstrual cycle and hormonal medicaments, can in-time generatin is accurate, reliable standardization result is assessed
Ovary reserve；For sensitivity, the change of Ovary reserve can be reflected earlier.Therefore, AMH is current prediction ovary
Reaction, the most ideal biological mark for assessing Ovary reserve.
Current method of testing is complex, and unsuitable disposable uses.
The content of the invention
To overcome the deficiency, it is an object of the invention to provide a kind of anti-Miao Le pipes hormone (AMH) detection means,
The technical solution adopted for the present invention to solve the technical problems is：A kind of anti-Miao Le pipes hormone (AMH) detection means,
Including multiple detection boxes, multiple reagent bottles, detection box is respectively used to sample detection, blank zeroing detection, contrasting detection, reagent bottle
The contrast agent of various concentrations is inside respectively provided with, the detection box includes shell, sample pad, nitrocellulose filter, adsorptive pads, institute
State and nitrocellulose filter is provided with shell, one end connection sample pad of nitrocellulose filter, other end connection adsorptive pads, nitric acid is fine
The plain film of dimension, sample pad, adsorptive pads are faced the wall and meditated by the upper table of shell and closely forced together with lower surface wall, and sample is located on the shell
The position of pad is provided with sample well, is provided with peep hole positioned at the position of nitrocellulose filter, the nitrocellulose filter is provided with inspection
Survey line and quality inspection line, detection line and quality inspection line expose at peep hole.
Specifically, the detection box is at least provided with six.
Specifically, the contrast agent be divided into the negative serum without AMH, low concentration AMH serum, high concentration AMH serum,
Middle concentration AMH serum.
Specifically, the AMH monoclonal antibodies and quality inspection line conjugates of mark collaurum are coated with the sample pad.
Specifically, the detection line is fixed with the 2nd AMH monoclonal antibodies, quality inspection line and is fixed with rabbit anti-mouse igg antibody.
Specifically, shading cover is provided with above the shell, two side inside walls of shading cover are provided with connection bump, shell
Two sides correspondence connection bump at be provided with connecting groove.
Specifically, one end of the shell is provided with connecting plate, connecting plate of the connecting plate provided with label, or multiple shells
By rivet interlacement together, sequencing numbers are facilitated.
A kind of anti-Miao Le pipes hormone (AMH) detection method：
Step one：Multiple detection boxes in a set of obtain to be made with a collection of reagent, select 2 testing cassetes of wherein at least to instill
Sample detection；1 testing cassete instills the negative serum without AMH, as blank detection；1 testing cassete instills low concentration AMH blood
Clearly, as demarcation numerical value；Concentration AMH serum during 1 testing cassete is instilled, is used as demarcation numerical value；1 testing cassete instills high concentration
AMH serum, is used as demarcation numerical value；In titration process, shading cover is pulled out laterally, exposes sample well, is instilled from sample well
Sample or reagent；
Step 2：Sample and reagent are all instilled finish after, shading cover is pushed to, lucifuge wait 10-15mim, wait question response；
Step 3：Each detection box is respectively put into collaurum and reads instrument measure photometric signal, fits within standard curve, enters
Row contrast, is modified using blank detection box demarcation numerical value.
The invention has the advantages that：Convenient use, can disposable detected, and ensure that testing result
Accurately, interpretation is conducive to.
Brief description of the drawings
Fig. 1 is the testing cassete structural representation of the embodiment of the present invention one.
Fig. 2 is the testing cassete internal structure schematic diagram of the embodiment of the present invention one.
Fig. 3 is the testing cassete structural representation of the embodiment of the present invention two.
Fig. 4 is the structural representation that the testing cassete of the embodiment of the present invention two fastens shading cover.
Fig. 5 is the structural representation of the testing cassete of the embodiment of the present invention three.
1 shell in figure, 11 connecting grooves, 12 shading covers, 2 peep holes, 3 wells, 4 connecting plates, 5 sample pads, 6 nitric acid are fine
The plain film of dimension, 61 detection lines, 62 quality inspection lines, 7 adsorptive pads, 8 rivets.
Embodiment
In conjunction with the accompanying drawings, the present invention is further explained in detail.
Embodiment one
A kind of anti-Miao Le pipes hormone (AMH) detection means as shown in Figure 1 and Figure 2, including multiple detection boxes, multiple reagents
Bottle, detection box is respectively used to be respectively provided with pair of various concentrations in sample detection, blank zeroing detection, contrasting detection, reagent bottle
Than reagent, the detection box includes being provided with nitric acid in shell 1, sample pad 5, nitrocellulose filter 6, adsorptive pads 7, the shell 1
Cellulose membrane 6, one end connection sample pad 5 of nitrocellulose filter 6, other end connection adsorptive pads 7, nitrocellulose filter 6, sample
Pad 5, adsorptive pads 7 are faced the wall and meditated by the upper table of shell and closely forced together with lower surface wall, and the position of sample pad 5 is located on the shell 1
Sample well 3 has been installed, peep hole 2 has been provided with positioned at the position of nitrocellulose filter 6, the nitrocellulose filter 6 is provided with detection
Line 61 and quality inspection line 62, detection line 61 and quality inspection line 62 expose at peep hole 2.
Specifically, the detection box is at least provided with six.
Specifically, the contrast agent be divided into the negative serum without AMH, low concentration AMH serum, high concentration AMH serum,
Middle concentration AMH serum, the negative serum without AMH is used as dummy, low concentration AMH serum, high concentration AMH serum,
Reagent is used middle concentration AMH serum as a comparison, and wherein low concentration AMH serum 1ng/mL containing AMH, high concentration AMH serum contains
AMH 3.5ng/mL, middle concentration AMH serum 6ng/mL containing AMH.
Specifically, the AMH monoclonal antibodies 61 and quality inspection line 62 that mark collaurum is coated with the sample pad 5 are coupled
Thing.
Specifically, the detection line 61 is fixed with the 2nd AMH monoclonal antibodies, from the AMH monoclonal antibodies of commercialization,
Rabbit anti-mouse igg antibody is fixed with quality inspection line 62, from the rabbit anti-mouse igg antibody of commercialization.
Specifically, the anti-Miao Leguanshi hormones of purchaser AMH, blank is done after purchase over-65s women negative serum mixing
Sample, standard AMH antigenic dilutions are done using negative serum, and final concentration takes every part of concentration samples between 1-1000ng/mL,
It is added on AMH colloidal gold immuno-chromatography test paper strips, after 15min, reads instrument with collaurum and determine photometric signal, every part of concentration sample
Product do 3-5 group parallel laboratory tests, and abscissa X is with different AMH standard protein content concns, strong with corresponding T lines photometric signal
Degree is ordinate Y, the standard curve of drafting.
A kind of anti-Miao Le pipes hormone (AMH) detection method：
Step one：Multiple detection boxes in a set of obtain to be made with a collection of reagent, select 2 testing cassetes of wherein at least to instill
Sample detection；1 testing cassete instills the negative serum without AMH, as blank detection；1 testing cassete instills low concentration AMH blood
Clearly, as demarcation numerical value；Concentration AMH serum during 1 testing cassete is instilled, is used as demarcation numerical value；1 testing cassete instills high concentration
AMH serum, is used as demarcation numerical value；In titration process, shading cover 12 is pulled out laterally, exposes sample well 3, from sample well 3
Instill sample or reagent；
Step 2：Sample and reagent are all instilled finish after, shading cover 12 is pushed to, lucifuge waits 10-15mim, waited anti-
Should；
Step 3：Each detection box is respectively put into collaurum and reads instrument measure photometric signal, fits within standard curve, enters
Row contrast, is modified using blank detection box demarcation numerical value.
Embodiment two
Specifically, shading cover 12 is provided with above the shell 1, two side inside walls of shading cover 12 are convex provided with connecting
Rise, connecting groove 11 is provided with two sides correspondence connection bump of shell 1.
Embodiment three
Specifically, one end of the shell 1 is provided with connecting plate 4, and the connecting plate 4 of multiple shells 1 is connected to one by rivet 8
Rise, facilitate sequencing numbers.
The present invention is not limited to the embodiment, and anyone should learn that the structure made under the enlightenment of the present invention becomes
Change, the technical schemes that are same or similar to the present invention are each fallen within protection scope of the present invention.
The technology of the invention not being described in detail, shape, construction part are known technology.
Claims (8)
1. a kind of anti-Miao Le pipes hormone (AMH) detection means, it is characterised in that：Including multiple detection boxes, multiple reagent bottles, detection
Box is respectively used to be respectively provided with the contrast agent of various concentrations in sample detection, blank zeroing detection, contrasting detection, reagent bottle,
The detection box includes being provided with nitrocellulose filter, nitric acid in shell, sample pad, nitrocellulose filter, adsorptive pads, the shell
One end connection sample pad of cellulose membrane, other end connection adsorptive pads, nitrocellulose filter, sample pad, adsorptive pads are by shell
Upper table is faced the wall and meditated and closely forced together with lower surface wall, sample well is provided with positioned at the position of sample pad on the shell, positioned at nitric acid
The position of cellulose membrane is provided with peep hole, and the nitrocellulose filter is provided with detection line and quality inspection line, detection line and quality inspection line
Expose at peep hole.
2. a kind of anti-Miao Le pipes hormone (AMH) detection means according to claim 1, it is characterised in that：The detection box
At least provided with six.
3. a kind of anti-Miao Le pipes hormone (AMH) detection means according to claim 1, it is characterised in that：Described pair is had a competition
Agent is divided into the negative serum without AMH, low concentration AMH serum, high concentration AMH serum, middle concentration AMH serum.
4. a kind of anti-Miao Le pipes hormone (AMH) detection means according to claim 1, it is characterised in that：The sample pad
On be coated with mark collaurum AMH monoclonal antibodies and quality inspection line conjugates.
5. a kind of anti-Miao Le pipes hormone (AMH) detection means according to claim 1, it is characterised in that：The detection line
It is fixed with the 2nd AMH monoclonal antibodies, quality inspection line and is fixed with rabbit anti-mouse igg antibody.
6. a kind of anti-Miao Le pipes hormone (AMH) detection means according to claim 1, it is characterised in that：The shell
Shading cover is provided with above, and two side inside walls of shading cover are provided with connection bump, two sides correspondence connection bump of shell
Provided with connecting groove.
7. a kind of anti-Miao Le pipes hormone (AMH) detection means according to claim 1, it is characterised in that：The shell
One end is provided with connecting plate, and connecting plate is provided with label, or multiple shells connecting plate by rivet interlacement together, convenient row
Sequence is numbered.
8. a kind of anti-Miao Le pipes hormone (AMH) detection method, it is characterised in that：Comprise the following steps：
Step one：Multiple detection boxes in a set of obtain to be made with a collection of reagent, select 2 testing cassetes of wherein at least to instill sample
Detection；1 testing cassete instills the negative serum without AMH, as blank detection；1 testing cassete instills low concentration AMH serum,
It is used as demarcation numerical value；Concentration AMH serum during 1 testing cassete is instilled, is used as demarcation numerical value；1 testing cassete instills high concentration AMH blood
Clearly, as demarcation numerical value；In titration process, shading cover is pulled out laterally, exposes sample well, from sample well instill sample or
Person's reagent；
Step 2：Sample and reagent are all instilled finish after, shading cover is pushed to, lucifuge wait 10-15mim, wait question response；
Step 3：Each detection box is respectively put into collaurum and reads instrument measure photometric signal, is fitted within standard curve, progress pair
Than being modified using blank detection box demarcation numerical value.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710568815.3A CN107192827A (en) | 2017-07-13 | 2017-07-13 | A kind of anti-Miao Le pipes hormone（AMH）Detection means and method |
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| CN201710568815.3A CN107192827A (en) | 2017-07-13 | 2017-07-13 | A kind of anti-Miao Le pipes hormone（AMH）Detection means and method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112908475A (en) * | 2021-04-22 | 2021-06-04 | 北京大学第三医院（北京大学第三临床医学院） | System for assessing ovarian reserve function in a subject |
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Application publication date: 20170922 |