CN107158209A - It is a kind of to treat herbal mixture biofermentation preparation of bird flu and preparation method thereof - Google Patents
It is a kind of to treat herbal mixture biofermentation preparation of bird flu and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to technical field of traditional Chinese medicines, it is related to and a kind of treats herbal mixture biofermentation preparation of bird flu and preparation method thereof.The raw material of the preparation includes in parts by weight：25 30 parts of 15 25 parts of the capsule of weeping forsythia, 20 28 parts of radix scutellariae, 15 25 parts of the bighead atractylodes rhizome, 30 40 parts of gypsum, 3 10 parts of radix glycyrrhizae, 15 20 parts of the wind-weed and cape jasmine.The herbal mixture biofermentation preparation of the treatment bird flu of the present invention, traditional Chinese medicinal components scientific compatibility, drug effect greatly enhances compared with traditional Chinese medicine drug effect, can effectively suppress and kill the breeding of avian influenza virus, effectively treat bird flu.The preparation method of the herbal mixture biofermentation preparation of the treatment bird flu of the present invention, after active ingredient in traditional Chinese medicinal components is extracted, comprehensive fermentation through special flora again, the drug effect for the herbal mixture fermentation preparation produced improves 20 25 times compared with traditional Chinese medicine liquid, macromolecular substances are changed into small molecule, toxicity is decomposed, taste improves, beneficial to absorption；Other production cost reduces nearly 70%, saving Chinese medicaments material more than 80%.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicines, it is related to a kind of herbal mixture biofermentation preparation and its system for treating bird flu
Preparation Method.
Background technology
Bird flu is the abbreviation of Bird Flu, and it is a kind of acute as caused by a kind of hypotype of influenza A virus
Infectious disease, can also infect the mankind, be set to category A infectious disease, also known as fowl plague or European checken pest by International Office of Epizootics.People infects
Symptom afterwards is mainly shown as hyperpyrexia, cough, runny nose, myalgia etc., most with serious pneumonia, and the severe patient heart, kidney etc. are a variety of
Organ failure causes death, and case fatality rate is very high, and usual people's infection bird flu death rate is about 33%.This disease can by alimentary canal,
The number of ways such as respiratory tract, skin injury and eye conjunctiva are propagated.
Nowadays the expansion development of poultry farming is big to the pharmaceutical requirements amount for preventing and treating bird flu, but at present in
The restriction of production of medicine, makes the low production efficiency of required treatment by Chinese herbs medicine, and drug effect is low.
The content of the invention
Based on problem above, one purpose of the present invention is to provide a kind of herbal mixture biofermentation system for treating bird flu
Agent；
A further object is to provide a kind of method for the herbal mixture biofermentation preparation for preparing above-mentioned treatment bird flu, the system
Preparation Method utilizes the bacterium with prominent drug effect, and disposably ferment Chinese traditional compound medicine, and efficient Chinese medicine fermented liquid, Ke Yiyou is made
Effect extracts the active component in Chinese medicine, significantly increases the drug effect of Chinese medicine preparation, realizes best therapeutic effect, while in solving
The bottleneck problem of medicine preparation industrial mass production.
According to the herbal mixture biofermentation preparation of the treatment bird flu of the present invention, the raw material of the Chinese medicine is in parts by weight
Including：15-25 parts of the capsule of weeping forsythia, 20-28 parts of radix scutellariae, 15-25 parts of the bighead atractylodes rhizome, 30-40 parts of gypsum, 3-10 parts of radix glycyrrhizae, 15-20 parts of the wind-weed
With 25-30 parts of cape jasmine.
According to the herbal mixture biofermentation preparation of the treatment bird flu of the present invention, further preferably, the original of the Chinese medicine
Material includes in parts by weight：23 parts of the capsule of weeping forsythia, 23 parts of radix scutellariae, 23 parts of the bighead atractylodes rhizome, 36 parts of gypsum, 6 parts of radix glycyrrhizae, 18 parts of the wind-weed and cape jasmine
27 parts.
According to the preparation method of the herbal mixture biofermentation preparation of the treatment bird flu of the present invention, the raw material of the Chinese medicine
Include in parts by weight：15-25 parts of the capsule of weeping forsythia, 20-28 parts of radix scutellariae, 15-25 parts of the bighead atractylodes rhizome, 30-40 parts of gypsum, 3-10 parts of radix glycyrrhizae,
25-30 parts of 15-20 parts of the wind-weed and cape jasmine.
The preparation method comprises the following steps：
(1) Chinese medicine material fine powder is prepared, will be crushed after Chinese medicine material cleaning, drying；
(2) decoction is extracted, by Chinese medicine material fine powder boiling made from step (1) and is filtered, decoction is obtained, what is obtained is described
The weight ratio of decoction and the Chinese medicine material is 8-12:1；
(3) sterilize, the decoction that step (2) is obtained carries out sterilizing；
(4) balanced nutritious source, various nutrition source datas are detected to the decoction after step (3) sterilizing, such as carbon, nitrogen,
Phosphorus, potassium and other trace elements, according to actual carbon-nitrogen ratio in decoction, carbon content adds carbon source when few, and nitrogen content adds nitrogen when few
Source, balanced carbon-nitrogen ratio to 30-35:1；
(5) ferment, zymophyte is added into the decoction behind step (4) balanced nutritious source, is fermented, into the decoction
Active component be more than or equal to 11wt% when, obtain it is described treatment bird flu herbal mixture biofermentation preparation.
According to the preparation method of the herbal mixture biofermentation preparation of the treatment bird flu of the present invention, in can effectively extracting
Active component in medicine raw material, it is ensured that the drug effect of the obtained Chinese medicine preparation treatment bird flu is farthest retained.
According to the present invention treatment bird flu herbal mixture biofermentation preparation preparation method, in order to ensure it is described in
Medicine raw material is prepared in step (1), and the Chinese medicine material crosses 80-150 mesh sieves after crushing.
According to the preparation method of the herbal mixture biofermentation preparation of the treatment bird flu of the present invention, in order to by the Chinese medicine
The effective active composition of raw material carries out high efficiency extraction, the excellent decoction of drug effect is obtained, in step (2), by the Chinese medicine material
Fine powder is fitted into the retort pouch of 180-250 mesh boiling 2-3 hours, after then drowning oneself 2-3 hours, takes supernatant, obtains decoction.
According to the preparation method of the herbal mixture biofermentation preparation of the treatment bird flu of the present invention, in order to remove in decoction
Miscellaneous bacteria, the drug effect of decoction is not influenceed, in step (3), the decoction that step (2) is obtained sterilizes 20-40 at 95-100 DEG C
Minute.
According to the preparation method of the herbal mixture biofermentation preparation of the treatment bird flu of the present invention, the carbon source based on addition
Decoction drug effect is not influenceed with nitrogen source, while purpose harmless to the body, in step (4), the carbon source is glucose, the nitrogen
Source is peptone.
According to the preparation method of the herbal mixture biofermentation preparation of the treatment bird flu of the present invention, in order to keep higher
Fermentation efficiency, enhancing drug effect, in step (5), the weight ratio of the zymophyte and the decoction is 4-5:100.
According to the preparation method of the herbal mixture biofermentation preparation of the treatment bird flu of the present invention, in step (5), institute
It is that bacillus subtilis or black-koji mould pass through melting that cell fusion is obtained with Resedaceae plant or feverfew to state zymophyte
Close cell fermentation bacterium.The herbal mixture biofermentation preparation tool of the treatment bird flu of described fused cell zymophyte fermentation
There is very strong drug effect, than general without 20-25 times of the Chinese medicine preparation drug effect enhancing prepared by present invention fermentation.The sweet-scented osmanthus
Careless section plant is fructus forsythiae or the fruit of glossy privet, and the feverfew is wild feverfew, climbing groundsel or the bighead atractylodes rhizome.
According to the preparation method of the herbal mixture biofermentation preparation of the treatment bird flu of the present invention, the fused cell hair
Yeast-like fungi is obtained by polyethylene glycol inducing cell fusion method.
According to the preparation method of the herbal mixture biofermentation preparation of the treatment bird flu of the present invention, in order to ensure the medicine
Liquid ferments under suitable pH environment, is additionally included in before step (5) fermentation, detects the pH value of the decoction, adds food-grade pH
The step of conditioning agent, wherein, the pH for adjusting the decoction is 6.5-7.5.The food-grade pH adjusting agent preferably calcium hydroxide, breast
Sour calcium or tricalcium phosphate.
According to the preparation method of the herbal mixture biofermentation preparation of the treatment bird flu of the present invention, in order to ensure the medicine
Liquid can fully ferment, and obtain the fermented tcm of quality better, make middle the effective elements of the medicine be more easy to be absorbed, and strengthen drug effect, and
Decompose Chinese materia medica tocixity, improve Chinese medicine taste, in step (5), in the bar of 25-37 DEG C, 30-40rpm, dissolved oxygen amount for 5-6mL/L
Fermented 68-75 hours under part.
For the ease of conveniently understanding technical scheme, the traditional Chinese medicinal components and effect being related in the present invention are done briefly
Explanation：
The capsule of weeping forsythia：It is bitter in taste, it is slightly cold, primary efficacy is clearing heat and detoxicating, dispersing swelling and dissipating binds.For treating ulcer, scrofula, acute mastitis,
Erysipelas, anemopyretic cold, warm disease is from the beginning of warm is joined the army, hyperpyrexia polydipsia, coma hair spot, heat gonorrhea renal shutdown.
Radix scutellariae：It is bitter in taste, tremble with fear, primary efficacy is heat-clearing and damp-drying drug, purging intense heat and detonicating, hemostasis is antiabortive.For treating damp-warm syndrome, heat
Temperature evil of vomitting uncomfortable in chest, damp and hot feeling of fullness, rush down dysentery, jaundice, cough with lung heat, hyperpyrexia polydipsia, blood-head tells nosebleed, carbuncle sore tumefacting virus, fetal irritability.
The bighead atractylodes rhizome：It is bitter in taste, sweet, temperature, primary efficacy is strengthening the spleen and replenishing qi, and eliminate dampness and have diuretic effect, hidroschesis is antiabortive.It is insufficiency of the spleen for treating
Deficiency of food, abdominal distention diarrhea, phlegm retention anti-dazzle nervous, oedema, spontaneous perspiration, fetal irritability.
Gypsum：Nature and flavor are sweet, pungent, and Great Cold, primary efficacy is clearing heat-fire, and relieving restlessness is quenched the thirst.It is high for treating fever caused by exogenous pathogenic factors
Hot polydipsia, dyspnea and cough due to lung-heat, stomach fire is high to contain, and has a headache, toothache.
Radix glycyrrhizae：Nature and flavor are sweet, put down, and primary efficacy is invigorates the spleen and benefits qi, clearing heat and detoxicating, expelling phlegm and arresting coughing, relieving spasm to stop pain, reconcile all
Medicine.Weakness of the spleen and the stomach for treating, lassitude hypodynamia, palpitation, coughing with a lot of sputum, gastral cavity abdomen, four limbs contraction, carbuncle sore tumefacting virus delay
Solve drug toxicity, strong.
The wind-weed：It is bitter in taste, sweet, tremble with fear, primary efficacy is clearing heat-fire, fluid dryness.For fever caused by exogenous pathogenic factors, hyperpyrexia polydipsia,
Lung-heat type cough, osteopyrexia and fever, Heat Diabetes, dry constipation of intestines.
Cape jasmine：It is bitter in taste, tremble with fear, primary efficacy is：Purging intense heat relieving restlessness, reducing fever and causing diuresis, removing pattogenic heat from the blood and toxic material from the body.It is yellow for Vexed
Subcutaneous ulcer urinates red, blood strangury and dry pain, blood-head tells nosebleed, red eye, swell pain, pathogenic fire,toxin and furuncles；Control bruise pain outside.
The present invention treatment bird flu herbal mixture biofermentation preparation focus on inducing diaphoresis, removing toxic substances, heat-clearing, purging intense heat, sterilization,
Resolving sputum, invigorating the spleen drive it is wet, from outward appearance to inner essence, treat both principal and secondary aspect of disease, strengthening vital QI to eliminate pathogenic factors, Pingyin sun, correcting the deficiency and excess, integrated regulation is carried out comprehensively, makes flesh
Body reaches best-of-breed functionality state.Capsule of weeping forsythia heat-clearing, radix scutellariae in the Chinese medicine material of the present invention mutually make with the capsule of weeping forsythia, cape jasmine, strengthen heat-clearing
Function, while clearing lung-heat liver and gall are hot；Radix glycyrrhizae is clearing heat and detoxicating, gypsum clearing away the stomach-heat, the bighead atractylodes rhizome and radix glycyrrhizae mutual reinforcement between, strengthening the spleen and replenishing qi, the wind-weed with it is sweet
Careless mutual reinforcement between, clearing stomach ease constipation.
The administrated method of herbal mixture biofermentation preparation of the treatment bird flu of the present invention is：Prevent poultry bird flu：
By the herbal mixture biofermentation preparation and water of the treatment bird flu of the present invention with 1:1000 volume ratio mix and convert, gives poultry drink
With；Treat poultry bird flu：With the herbal mixture biofermentation preparation of the treatment bird flu of the present invention with 2:1000 volume ratio
Mix and convert, drinks to ill poultry.
Beneficial effects of the present invention are：
According to the present invention treatment bird flu herbal mixture biofermentation preparation, traditional Chinese medicinal components scientific compatibility, drug effect compared with
Traditional Chinese medicine drug effect is greatly enhanced, and can effectively be suppressed and be killed the breeding of avian influenza virus, effectively treat bird flu.
The preparation method of the herbal mixture biofermentation preparation of the treatment bird flu of the present invention, will be effective in traditional Chinese medicinal components
After composition is extracted, then the comprehensive fermentation through special flora, the drug effect for the herbal mixture fermentation preparation produced is compared with traditional Chinese medicine
Liquid improves 20-25 times, and macromolecular substances are changed into small molecule, decomposes toxicity, improves taste, beneficial to absorption；It is produced into addition
Originally nearly 70%, saving Chinese medicaments material more than 80% are reduced.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical scheme will be carried out below
Detailed description.Obviously, described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are resulting on the premise of creative work is not made to be owned
Other embodiment, belongs to the scope that the present invention is protected.
Following examples are with 1 part for 1 ton.
Embodiment 1
The present invention provides a kind of herbal mixture biofermentation preparation for treating bird flu, and the raw material of the Chinese medicine is with parts by weight
Meter includes：25 parts of 3 parts of 15 parts of the capsule of weeping forsythia, 28 parts of radix scutellariae, 15 parts of the bighead atractylodes rhizome, 40 parts of gypsum and radix glycyrrhizae, 20 parts of the wind-weed and cape jasmine.
The preparation method of the Chinese medicine preparation, comprises the following steps：
(1) Chinese medicine material fine powder is prepared, 150 mesh sieves are crossed after the Chinese medicine material is dried, crushed；
(2) decoction is extracted, Chinese medicine material fine powder made from step (1) is loaded to the retort pouch of 180 mesh, is steamed in vapor can
Boil 3 hours and extract decoction, then decoction is entered after settling tank drowns oneself 2 hours, supernatant is taken, obtains decoction, the obtained medicine
The weight ratio of liquid and the Chinese medicine material is 8:1；
(3) sterilize, the decoction that step (2) is obtained moves into fermentation tank, sterilizing is carried out at 100 DEG C 20 minutes；
(4) balanced nutritious source, various nutrition source datas are detected by the decoction after step (3) sterilizing, such as carbon, nitrogen,
Phosphorus, potassium and other trace elements, add glucose, balanced carbon-nitrogen ratio to 30:1；
(5) ferment, food-grade pH adjusting agent is added into the decoction behind step (4) balanced nutritious source and adjusts the decoction
PH be 6.5, then add fused cell zymophyte, 75 fermented under conditions of 37 DEG C, rotating speed 30rpm, dissolved oxygen amount are 5mL/L
When active component of the hour into the decoction is more than or equal to 11wt%, the herbal mixture biology hair of the treatment bird flu is obtained
Ferment preparation.
The cell fusion zymophyte is that bacillus subtilis is melted with capsule of weeping forsythia plant cell by polyethylene glycol inducing cell
Legal to be made, the weight ratio of the cell fusion zymophyte and the decoction is 5:100.
Embodiment 2
The present invention provides a kind of herbal mixture biofermentation preparation for treating bird flu, and the raw material of the Chinese medicine is with parts by weight
Meter includes：30 parts of 10 parts of 25 parts of the capsule of weeping forsythia, 20 parts of radix scutellariae, 25 parts of the bighead atractylodes rhizome, 30 parts of gypsum and radix glycyrrhizae, 15 parts of the wind-weed and cape jasmine.
The preparation method of the Chinese medicine preparation, comprises the following steps：
(1) Chinese medicine material fine powder is prepared, 80 mesh sieves are crossed after the Chinese medicine material is dried, crushed；
(2) decoction is extracted, Chinese medicine material fine powder made from step (1) is loaded to the retort pouch of 250 mesh, is steamed in vapor can
Boil 2 hours and extract decoction, then decoction is entered after settling tank drowns oneself 3 hours, supernatant is taken, obtains decoction, the obtained medicine
The weight ratio of liquid and the Chinese medicine material is 12:1；
(3) sterilize, the decoction that step (2) is obtained moves into fermentation tank, sterilizing is carried out at 95 DEG C 40 minutes；
(4) balanced nutritious source, various nutrition source datas are detected by the decoction after step (3) sterilizing, such as carbon, nitrogen,
Phosphorus, potassium and other trace elements, add peptone, balanced carbon-nitrogen ratio to 35:1；
(5) ferment, food-grade pH adjusting agent is added into the decoction behind step (4) balanced nutritious source and adjusts the decoction
PH be 7.5, then add fused cell zymophyte, 68 fermented under conditions of 25 DEG C, rotating speed 36rpm, dissolved oxygen amount are 6mL/L
When active component of the hour into the decoction is more than or equal to 11wt%, the herbal mixture biology hair of the treatment bird flu is obtained
Ferment preparation.
The cell fusion zymophyte is that black-koji mould is merged with wild feverfew plant cell by polyethylene glycol inducing cell
Method is made, and the weight ratio of the cell fusion zymophyte and the decoction is 4:100.
The cell fusion zymophyte can also be by the plant of black-koji mould and the capsule of weeping forsythia or the fruit of glossy privet or climbing groundsel or the bighead atractylodes rhizome
Cell is made by polyethylene glycol inducing cell fusion method.
Embodiment 3
The present invention provides a kind of herbal mixture biofermentation preparation for treating bird flu, and the raw material of the Chinese medicine is with parts by weight
Meter includes：27 parts of 23 parts of the capsule of weeping forsythia, 23 parts of radix scutellariae, 23 parts of the bighead atractylodes rhizome, 36 parts of gypsum, 6 parts of radix glycyrrhizae, 18 parts of the wind-weed and cape jasmine.
The preparation method of the Chinese medicine preparation, comprises the following steps：
(1) Chinese medicine material fine powder is prepared, 100 mesh sieves are crossed after the Chinese medicine material is dried, crushed；
(2) decoction is extracted, Chinese medicine material fine powder made from step (1) is loaded to the retort pouch of 200 mesh, is steamed in vapor can
Boil 2 hours and extract decoction, then decoction is entered after settling tank drowns oneself 2 hours, supernatant is taken, obtains decoction, the obtained medicine
The weight ratio of liquid and the Chinese medicine material is 10:1；
(3) sterilize, the decoction that step (2) is obtained moves into fermentation tank, sterilizing is carried out at 98 DEG C 30 minutes；
(4) balanced nutritious source, various nutrition source datas are detected by the decoction after step (3) sterilizing, such as carbon, nitrogen,
Phosphorus, potassium and other trace elements, add glucose, balanced carbon-nitrogen ratio to 32:1；
(5) ferment, food-grade pH adjusting agent is added into the decoction behind step (4) balanced nutritious source and adjusts the decoction
PH be 6.8, then add fused cell zymophyte, 72 fermented under conditions of 30 DEG C, rotating speed 40rpm, dissolved oxygen amount are 5mL/L
When active component of the hour into the decoction is more than or equal to 11wt%, the herbal mixture biology hair of the treatment bird flu is obtained
Ferment preparation.
The cell fusion zymophyte is that bacillus subtilis passes through polyethylene glycol inducing cell with climbing groundsel plant cell
Fusion method is made, and the weight ratio of the cell fusion zymophyte and the decoction is 5:100.
The cell fusion zymophyte can also be thin by bacillus subtilis and the plant of the fruit of glossy privet or wild feverfew or the bighead atractylodes rhizome
Born of the same parents are made by polyethylene glycol inducing cell fusion method.
Experimental example
First, materials and methods
1.1 strain
H9 viral prevalences strain 1167, is preserved by Shandong Academy of Agricultural Sciences, determines median lethal of the virus to SPF chicken embryos
Measure as (EID50)5.1×109.5/ mL, to SPF chicken median lethal doses (LD50).IBV viruses are preserved by Shandong Academy of Agricultural Sciences,
It is (LD to determine to the median lethal dose of SPF chickens50:6.1×109.5/mL)
1.2 chicken embryos and chicken
SPF chicken embryos, SPF chickens are purchased from Si Paifurui Qin Ye Science and Technology Ltd.s of Shandong Province.SPF fowl raisings are in stainless steel malleation
In isolator, air passes through filtration sterilization, and drinking-water and feed enter receptacle, Suo Youshi after disinfecting by transferring cabin
Test and carried out in P3 laboratory, challenge test is carried out in poultry research institute of Shandong academy of agricultural sciences SPF cages.
1.3 experimental method
1.3.1 the herbal mixture biofermentation preparation of the treatment bird flu prepared by 1-3 of the embodiment of the present invention is named as L,
Study its inhibitory action replicated to H9 avian influenza virus in chicken embryo
H9 avian influenza virus according to 10 times of doubling dilutions, each dilution factor takes 500ul and the Chinese medicine preparation L of equivalent respectively
Mix, 30min is acted in room temperature, take 0.2ml mixed liquors to be inoculated with 9 age in days SPF chicken embryos, each dilution factor is inoculated with 8 pieces of SPF chicken embryos；
Control group directly uses 105.5EID5010 pieces of SPF chicken embryos of poison disease vaccination.Place sterile after 37 DEG C of incubator hatchings, 72h receive each
Chick embryo allantoic liquid, allantoic fluid HA-HI test is surveyed with 0.75% chicken red blood cell, and is recorded, as a result as shown in table 2.
1.3.2 Chinese medicine preparation L is to the IBV of SPF chickens virus, H9 avian influenza virus protest tests
SPF chickens are divided into 4 groups, every group 13, artificial challenge H9 and IBV virus are carried out during 12 age in days, after infection second
My god, manually gavage Chinese medicine preparation L, Continuous Observation 5 days, record its mental status, death condition, whether cough, dead dissection
Situations such as observing renal lesions, every group of random 3 anatomic observation lesion situations of picking after 7 days.After off-test, remaining chicken makes
Handled with autoclaving after euthanasia.
Experiment packet is as shown in table 1：
Table 1
| Group | Attack malicious situation | Chinese medicine preparation L dosages |
| I group | H9 and IBV viruses | 0.1ml/ is only |
| II group | H9 and IBV viruses | 0.3ml/ is only |
| III group | H9 and IBV viruses | 0.5ml/ is only |
| IV group | H9 and IBV viruses | - |
2nd, result of the test
The inhibitory action that 2.1 Chinese medicine preparation L are replicated to H9 avian influenza virus in chicken embryo
Experimental result is shown in Table 2, it can be seen that the viral dilution liquid of 10 times of dilutions of Chinese medicine preparation of the invention has suppression to imitate
Really, virus can not be replicated in chicken embryo, therefore, and Chinese medicine preparation of the invention has inhibition to avian influenza virus；The present invention's
Chinese medicine preparation suppresses the maximum concentration that is replicated in chicken embryo of low pathogenicity H9 avian influenza virus：5.1×108.5/ml。
Chinese medicine+virus inoculation chicken embryo result is as shown in table 2：
Table 2
| Chinese medicine preparation L stostes+times dilution virus | Chicken embryo HA blood clottings | Viral dilution gradient | |
| 1 | Chinese medicine preparation L+ equivalent 5.1 × 109.5/mL | ++++++++ | Virus stock solution used |
| 2 | Chinese medicine preparation L+ equivalent 5.1 × 108.5/mL | -------- | 10 times of dilutions |
| 3 | Chinese medicine preparation L+ equivalent 5.1 × 107.5/mL | -------- | 102Times dilution |
| 4 | Chinese medicine preparation L+ equivalent 5.1 × 106.5/mL | -------- | 103Times dilution |
| 5 | Chinese medicine preparation L+ equivalent 5.1 × 105.5/mL | -------- | 104Times dilution |
| 6 | Chinese medicine preparation L+ equivalent 5.1 × 104.5/mL | -------- | 105Times dilution |
| 7 | Chinese medicine preparation L+ equivalent 5.1 × 103.5/mL | -------- | 106Times dilution |
| 8 | Chinese medicine preparation L+ equivalent 5.1 × 102.5/mL | -------- | 107Times dilution |
| 9 | Chinese medicine preparation L+ equivalent 5.1 × 101.5/mL | -------- | 108Times dilution |
| 10 | Chinese medicine preparation L+ equivalent 5.1 × 100.5/mL | -------- | 109Times dilution |
| 11 | Control (the H9 viruses for being not added with Chinese medicine) | ++++++++ | 105.5EID50 |
Note："+", represents that each chick embryo allantoic liquid has coagulation, "-" represents each chick embryo allantoic liquid without coagulation
2.2 Chinese medicine preparation L are to the IBV of SPF chickens virus, H9 avian influenza virus protest tests
Attacked using H9 and IBV after poison 5 days, control group existing feed intake occurs and declines, coughs, flows in nose liquid symptom, experimental group
State symptom lighter than compareing, 3 death occurs in control group during observation, 1 death occurs in I group and II group of experiment, tests III group
Without death, find that piebald kidney, kidney swollen symptom, annulus trachealis bleeding, experimental group death meat occurs in control group to the dissection of dead chicken
There is kidney and swollen in chicken, slight piebald kidney, tracheorrhagia.Experiment packet is as shown in table 3：
Table 3
| Group | Sample size | Chinese medicine preparation L dosages | Extremely naughty quantity |
| I group | 13 | 0.1ml/ is only | 1 |
| II group | 13 | 0.3ml/ is only | 1 |
| III group | 13 | 0.5ml/ is only | 0 |
| IV group | 13 | - | 3 |
Above test result indicates that, Chinese medicine preparation L of the present invention has to IBV viruses and H9 avian influenza virus significant suppresses work(
Effect, can play a part of effectively treating and preventing avian influenza virus during home poultry raising.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of herbal mixture biofermentation preparation for treating bird flu, it is characterised in that the raw material of the Chinese medicine is with parts by weight
Meter includes：15-25 parts of the capsule of weeping forsythia, 20-28 parts of radix scutellariae, 15-25 parts of the bighead atractylodes rhizome, 30-40 parts of gypsum, 3-10 parts of radix glycyrrhizae, wind-weed 15-20
25-30 parts of part and cape jasmine.
2. the herbal mixture biofermentation preparation for the treatment of bird flu according to claim 1, it is characterised in that the Chinese medicine
Raw material include in parts by weight：23 parts of the capsule of weeping forsythia, 23 parts of radix scutellariae, 23 parts of the bighead atractylodes rhizome, 36 parts of gypsum, 6 parts of radix glycyrrhizae, 18 parts of the wind-weed and
27 parts of cape jasmine.
3. a kind of preparation method for the herbal mixture biofermentation preparation for treating bird flu, it is characterised in that the original of the Chinese medicine
Material includes in parts by weight：15-25 parts of the capsule of weeping forsythia, 20-28 parts of radix scutellariae, 15-25 parts of the bighead atractylodes rhizome, 30-40 parts of gypsum, radix glycyrrhizae 3-10
25-30 parts of part, 15-20 parts of the wind-weed and cape jasmine, the preparation method comprises the following steps：
(1) Chinese medicine material fine powder is prepared, Chinese medicine material is crushed；
(2) decoction is extracted, by Chinese medicine material fine powder boiling made from step (1) and is filtered, decoction is obtained；
(3) sterilize, the decoction that step (2) is obtained carries out sterilizing；
(4) balanced nutritious source, carbon source or nitrogen source, balanced carbon-nitrogen ratio to 30- are added into the decoction after step (3) sterilizing
35:1；
(5) ferment, add zymophyte to be fermented into the decoction behind step (4) balanced nutritious source, the work into the decoction
Property composition be more than or equal to 11wt%, obtain it is described treatment bird flu herbal mixture biofermentation preparation.
4. the preparation method of the herbal mixture biofermentation preparation for the treatment of bird flu according to claim 3, its feature exists
In in step (1), the Chinese medicine material crosses 80-150 mesh sieves after crushing.
5. the preparation method of the herbal mixture biofermentation preparation for the treatment of bird flu according to claim 3, its feature exists
In in step (2), the Chinese medicine material fine powder being fitted into the retort pouch of 180-250 mesh boiling 2-3 hours, then drowned oneself
After 2-3 hours, supernatant is taken, decoction is obtained.
6. the preparation method of the herbal mixture biofermentation preparation for the treatment of bird flu according to claim 3, its feature exists
In in step (3), the decoction that step (2) is obtained is sterilized 20-40 minutes at a temperature of 95-100 DEG C.
7. the preparation method of the herbal mixture biofermentation preparation for the treatment of bird flu according to claim 3, its feature exists
In in step (5), the weight ratio of the zymophyte and the decoction is 4-5:100.
8. the preparation method of the herbal mixture biofermentation preparation for the treatment of bird flu according to claim 3, its feature exists
In in step (5), the zymophyte is that bacillus subtilis or black-koji mould pass through with Resedaceae plant or feverfew
The fused cell zymophyte that cell fusion is obtained.
9. the preparation method of the herbal mixture biofermentation preparation for the treatment of bird flu according to claim 3, its feature exists
In being that 30-40rpm, dissolved oxygen amount are to ferment 68-75 hours under conditions of 5-6mL/L in 25-37 DEG C, rotating speed in step (5).
10. the preparation method of the herbal mixture biofermentation preparation for the treatment of bird flu according to claim 3, its feature exists
In, the step of adding food-grade pH adjusting agent before step (5) is fermented is additionally included in, wherein, the pH for adjusting the decoction is
6.5-7.5。
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109432287A (en) * | 2018-12-21 | 2019-03-08 | 济南亿民动物药业有限公司 | A kind of traditional Chinese medicine oral liquid and preparation method thereof of prevention and treatment poultry excessive heat in the system card |
| CN109620884A (en) * | 2019-01-28 | 2019-04-16 | 孟繁华 | A kind of preparation method of the active compound of targeted therapy bird flu |
| CN110237170A (en) * | 2019-07-05 | 2019-09-17 | 任辛 | A kind of preparation method of plant cell enzymatic hydrolyzed extract that treating bird flu |
| CN111544561A (en) * | 2020-06-08 | 2020-08-18 | 石家庄恒泰和动物药业有限公司 | Pharmaceutical composition for treating poultry influenza and preparation method thereof |
-
2017
- 2017-07-17 CN CN201710580956.7A patent/CN107158209A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 张锁献: "《畜禽疾病中草药防治》", 31 May 2015, 河北科学技术出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109432287A (en) * | 2018-12-21 | 2019-03-08 | 济南亿民动物药业有限公司 | A kind of traditional Chinese medicine oral liquid and preparation method thereof of prevention and treatment poultry excessive heat in the system card |
| CN109432287B (en) * | 2018-12-21 | 2021-06-04 | 济南亿民动物药业有限公司 | Traditional Chinese medicine oral liquid for preventing and treating excessive heat syndrome of poultry qi system and preparation method thereof |
| CN109620884A (en) * | 2019-01-28 | 2019-04-16 | 孟繁华 | A kind of preparation method of the active compound of targeted therapy bird flu |
| CN110237170A (en) * | 2019-07-05 | 2019-09-17 | 任辛 | A kind of preparation method of plant cell enzymatic hydrolyzed extract that treating bird flu |
| CN111544561A (en) * | 2020-06-08 | 2020-08-18 | 石家庄恒泰和动物药业有限公司 | Pharmaceutical composition for treating poultry influenza and preparation method thereof |
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Application publication date: 20170915 |