CN107088661A

CN107088661A - A kind of synthetic method of Nano Silver

Info

Publication number: CN107088661A
Application number: CN201710327955.1A
Authority: CN
Inventors: 袁玉国
Original assignee: Yangzhou University
Current assignee: Yangzhou University
Priority date: 2017-05-11
Filing date: 2017-05-11
Publication date: 2017-08-25

Abstract

A kind of synthetic method of Nano Silver, is related to the technical fields such as environment disinfected and veterinary clinic, and the DMSO solution of quercetin is reacted with silver nitrate aqueous solution mixing, obtains nano silver material.The size nano silver material average diameter of synthesis is 10nm, to drug-resistant pseudomonas aeruginosa and the Mlc of gold-coloured staphylococci（MIC）And bacteriocidal concentration（MBC）Respectively 1 μ g/mL and 2 μ g/mL, 2 μ g/mL and 4 μ g/mL, and Nano Silver effect 24h can completely inhibit resistance bacteria growing.Therefore, the nano silver material synthetic method is simple, can be mass-produced, there is obvious action effect to drug-fast bacteria.

Description

A kind of synthetic method of Nano Silver

Technical field

The present invention relates to the technical fields such as environment disinfected and veterinary clinic, and in particular to a kind of method of synthesizing nano-silver, It is directed to environment and plant's environment disinfected, sterilization and the Prevention Technique field for treating ox, goat mastitis veterinary clinic disease etc..

Background technology

Mastadenitis of cow is that a kind of inflammatory produced by mammary gland is stimulated by physics, chemistry, microorganism changes, and is milk cow Common disease and frequently-occurring disease.At present, the whole world there are about 2.2 hundred million more bovine cows, wherein about 1/3 suffers from various types of mammary gland Inflammation, the economic loss caused is annual up to 35,000,000,000 dollars.Surpassed the world average level in the incidence of disease of China's mastadenitis of cow, It is annual up to 1.35 hundred million yuan to the economic loss that China brings.Mastitis is mainly caused by a variety of unspecific pathogenic microorganisms, There are bacterium, fungi, virus and mycoplasma etc..Research shows that staphylococcus aureus, streptococcus and Escherichia coli account for whole milk More than the 90% of bovine mastitis case.Since being come out from the 40's of 20th century antibiotic, the treatment of mastitis is depended on Antibiotic, but antibiotherapy the drawbacks of expose it gradually.It is the appearance of antibody-resistant bacterium and changing for pathogenic bacteria first Become, decline antibiotic curative effect.Current research finds that staphylococcus aureus can hide in the hiding in macrophage and breast epithelium for a long time It is intracellular, and traditional antibiotic generally can not penetrate into cell or enter cell concentration it is too low and do not reach and control curative effect Really.Another drawback of antibiotherapy is the antibiotic residue problem in milk, and the antibiotic of Residues in Milk can induce people Hypersensitivity occurs for body, and great threat is caused to human health.And pure Chinese medicinal preparation, although noresidue, toxic side effect are small, but Its antibacterial efficacy can not show a candle to antibiotic.So, it is the task of top priority to find or invent more preferably medicine newly or reagent.

A large amount of with chemicalses such as antibiotic, disinfectant and bactericide use, and the microorganism as caused by drug resistance becomes Xenogenesis group is more and more, and the global microbial plague event thus triggered occurs again and again.In order to prevent microbial plague event Generation, research and development and application novel antibacterial material be a kind of effective method.It is anti-that traditional antiseptic can be divided into inorganic system Microbial inoculum, organic series antibacterial agent and all kinds of antiseptics of natural biological series antibacterial agent have respective advantage and disadvantage.Organic series antibacterial agent it is anti- Bacterium effect is good, with low cost, but its poor heat resistance, or even strong toxicity, can only typically be used for carrying out disposable sterilization or sterilizing.My god Right biomaterial is as antiseptic, and toxicity and environmental pollution are small, but are restricted by security and processing conditions, at present also not Mass market can be realized, is difficult to make nano-class antibacterial agent.With the continuous improvement of people's living standards, wishing to connect with people Tactile various life appliances energy automatic disinfection after by courses of infection is degerming, it is desirable to which the material of life appliance in itself can be chronically Discharge antiseptic or play a part of antiseptic.Numerous studies show that inorganic series antibacterial agent antibacterial spectrum width, toxicity is low, heat-resisting Property it is strong, be suitable for the antibiotic and sterilizing under the various living conditions of the mankind, and be especially suitable for and make nano-class antibacterial agent, inorganic antibacterial material Material has safe, and heat resistance and persistence are good, and the advantages of be not likely to produce the resistance to the action of a drug, from the 80's of 20th century with To obtain exploitation and application successively.Wherein, silver-colored inorganic antibacterial material is carried because its toxicity is low, antibacterial activity is high, has a broad antifungal spectrum, Current most widely used inorganic antibacterial material is turned into.

Invention or content

It is resistance to improve gold-coloured staphylococci and Pseudomonas aeruginosa etc. it is an object of the present invention to provide a kind of method of simple synthesizing nano-silver The killing effect of medicine bacterium.

The technical scheme is that：The DMSO solution of quercetin is reacted with silver nitrate aqueous solution mixing, acquirement is received Rice ag material.

Tests prove that the size nano silver material average diameter of synthesis is 10nm, to drug-resistant pseudomonas aeruginosa and golden grape The Mlc of coccus（MIC）And bacteriocidal concentration（MBC）Respectively 1 μ g/mL and 2 μ g/mL, 2 μ g/mL and 4 μ g/mL, and nanometer Silver effect 24h can completely inhibit resistance bacteria growing.Therefore, the nano silver material synthetic method is simple, can be mass-produced, right Drug-fast bacteria has obvious action effect.

Further, quercetin content is 0.5mg/mL in the DMSO solution of the quercetin.Quercetin has in DMSO Preferable dissolubility, beneficial to the progress of reaction.

Quercetin contains many phenolic hydroxyl group groups that can be combined with free radical, with antioxidation.And quercetin can be very Easily and quickly silver oxide ion, and effectively can prevent Nano Silver from assembling as sealer.Therefore, using the rubber of debita spissitudo Flavine can effectively synthesizing nano-silver material and with good stability as reducing agent.

Silver nitrate concentration is 1mM in the silver nitrate aqueous solution.Reacted, can had with 1mM silver nitrate aqueous solutions and quercetin The synthesizing nano-silver of effect, and can preferably control nano-Ag particles size.

The reaction is carried out under the conditions of 25~50 DEG C.At this temperature, the reduction reaction of Nano Silver can effectively be controlled Speed etc., controls nano-Ag particles size.Reaction is easier control reaction speed etc. under the conditions of 40 DEG C, obtains optimal size chi Very little nano-Ag particles.

Brief description of the drawings

Fig. 1 is the distribution map of the Nano Silver diameter synthesized.

Fig. 2 is the graph of a relation of nanometer silver concentration and bacterial action.

Fig. 3 is the graph of a relation that Nano Silver suppresses time and bacterial action.

Embodiment

1st, Nano Silver is prepared：

Weigh quercetin and be dissolved in the solution that 0.5mg/mL is made into DMSO.

Weigh silver nitrate powder and be dissolved in water and be configured to 1mM solution.

Quercetin solution is mixed in equal volume with silver nitrate solution, at 25~50 DEG C（It is preferred that 40 DEG C）At a temperature of stirring reaction 1h, in the process it can be seen that solution becomes dark brown by yellow, you can complete the synthesis of Nano Silver.

The dark brown mixed liquor that reaction is obtained moves into the 24h that dialysed in the bag filter of 12kDa sizes, then moves into solution In 50mL test tubes, freeze-drying produces nano silver material.

2nd, Nano Silver diameter is detected：

Appropriate amount of deionized water is added in the Nano Silver of acquirement and is made into 0.5mg/mL Nano Silver water mixed liquids, by transmission electron microscope and Particle diameter distribution instrument detects synthesizing nano-silver size.

As seen from Figure 1：Nano Silver diameter is distributed between 1 ~ 21nm, and Nano Silver average diameter is 10nm.

3rd, application of the Nano Silver on drug-fast bacteria：

Separated with Conventional bacteria separation method from the milk for suffering from mammitis sheep and obtain Pseudomonas aeruginosa and gold-coloured staphylococci, entered respectively Row experiment.

Bacterium is cultivated into 12h in 37 DEG C of nutrient broth nutrient solution, fresh medium culture 24h is added, bacterium solution from Supernatant is removed after the heart, precipitation is blown and beaten with deionized water disperses, and bacterium solution is diluted into 10⁶Individual/mL, adds nanometer into bacterium solution respectively Silver, is made under the conditions of 0,0.2,0.4,0.6,0.8,1,2 μ g/mL Nano silver solution, 37 DEG C and is further cultured for after 24h, different Time point（0、4、8、12、16、20、24h）100 μ L bacterium solutions are taken to calculate CFU respectively, to detect that the antibacterial of Nano Silver and sterilization are imitated Really.

Pseudomonas aeruginosa is respectively adopted and gold-coloured staphylococci carries out result of the test as shown in Figure 2,3.

As seen from Figure 2：Nano Silver is respectively 1 μ g/ to the minimum inhibitory concentration of drug-resistant pseudomonas aeruginosa and gold-coloured staphylococci ML and 2 μ g/mL, bacteriocidal concentration is respectively 2 μ g/mL and 4 μ g/mL.

As seen from Figure 3：Resistance bacteria growing can be completely inhibited after Nano Silver effect 24h.

Claims

1. a kind of synthetic method of Nano Silver, it is characterised in that the DMSO solution of quercetin is mixed into progress with silver nitrate aqueous solution Reaction, obtains Nano Silver.

2. the synthetic method of Nano Silver according to claim 1, it is characterised in that rubber is yellow in the DMSO solution of the quercetin Cellulose content is 0.5mg/mL.

3. the synthetic method of Nano Silver according to claim 1, it is characterised in that silver nitrate is dense in the silver nitrate aqueous solution Spend for 1mM.

4. the synthetic method of Nano Silver according to claim 1 or 2 or 3, it is characterised in that the reaction is in 25~50 DEG C of bars Carried out under part.

5. the synthetic method of Nano Silver according to claim 4, it is characterised in that carried out under the conditions of 40 DEG C.