CN107064044B - Method and device for rapidly detecting polyphenol content in tea extract - Google Patents
Method and device for rapidly detecting polyphenol content in tea extract Download PDFInfo
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
Abstract
The invention provides a method and a device for rapidly detecting the content of polyphenol in tea extract. Detecting the polyphenol content in the tea extract by adopting a conventional physicochemical analysis method; the semi-transmission detection device is used as a carrier of a sample, the miniature optical fiber device is used as a transmission carrier of a spectrum signal, and the portable near-infrared spectrometer collects semi-transmission spectrum data in the tea extracting solution; establishing a model for predicting the polyphenol content in the tea extract by combining the collected polyphenol content of the tea extract and spectral data with a chemometrics method; transmitting the spectral data to a computer client in real time through USB transmission; and (4) analyzing the spectral data at the computer client by combining the written model program, and displaying the polyphenol content in the tea extracting solution in real time. The technology of the invention is based on near infrared spectrum-USB transmission technology, can carry out rapid quantitative detection on the polyphenol content in the tea extract, can obviously improve the corresponding detection efficiency compared with the traditional physicochemical detection, and is more suitable for online detection and analysis in the field of tea deep processing.
Description
Technical Field
The invention relates to a detection technology and equipment for the polyphenol content in tea extract, in particular to a method and a device for realizing quantitative analysis of the polyphenol content in the tea extract based on a near infrared spectrum technology and a USB transmission technology.
Background
With the rapid development of the food industry and the improvement of the standard of living of people, tea beverages, which are representative of healthy drinks, are rapidly developing at present and becoming the most marketable beverages, excluding drinking water. Tea Polyphenols (TPs) are one of the most important functional components in tea leaves, account for about 20% -35% of dry matter of the tea leaves, are main components determining taste and color in tea extract, and are key substances determining tea quality. Therefore, the rapid detection and online monitoring of the polyphenol content in the tea extract are urgently needed in the tea beverage processing and production process.
At present, the national standard GB/T8313-2008 Fulin phenol reagent colorimetric method is mostly adopted to determine the content of tea polyphenol in tea, but the method is time-consuming, consumes chemical reagents, has complicated steps and is difficult to adapt to the requirement of real-time rapid detection in the tea beverage production process. Therefore, it is necessary to research a rapid detection method for accurately and rapidly determining the polyphenol content in the tea extract and develop corresponding detection equipment, which is of great significance to the field of tea deep processing.
The near infrared spectrum is an electromagnetic radiation wave between visible light (VIS) and mid-infrared light (MIR), the corresponding wavelength range is 780-2500 nm, ideal results are obtained when the near infrared spectrum is used for rapid nondestructive detection of the content of tea polyphenol in dry tea and qualitative identification of tea, and the application of the near infrared spectrum to detection of components in tea extract is rarely reported; meanwhile, qualitative and quantitative analysis research of tea leaves is mostly limited to Fourier near infrared (FT-NIR) detection operated in a laboratory, corresponding instruments are expensive, and online real-time analysis is difficult to realize.
For example, in the patent of 'a device and a method for rapidly and nondestructively detecting the component content of green tea' with Chinese patent application number 200810162600.2, the diffuse reflection method is adopted to measure the absorption spectrum of the green tea, and the chemometrics method is combined to realize the rapid and nondestructive detection of four components of amino acid, caffeine, tea polyphenol and water in the green tea; however, the device can not realize rapid determination of the internal components in the tea extract, and can not be used for field detection in the field of tea deep processing.
For example, in the 'optical path selection of near-infrared quantitative analysis of tea polyphenol in green tea soup', Liu Fu Li et al, the partial least square modeling method is adopted to perform modeling analysis on the near-infrared transmission spectrum in green tea soup, but the MPA Fourier transform near-infrared spectrometer of Germany Bruker optical instruments adopted by the research is expensive in price, large in size and not high in universality in the field of deep processing of tea leaves.
Therefore, the method for simply, conveniently and rapidly detecting the polyphenol content in the tea extract is found, and has important practical significance for meeting the requirement of the field of tea deep processing. The invention uses the semi-transmission detection device as a carrier for spectrum collection and the micro optical fiber device as a transmission carrier for spectrum signals, the portable near-infrared spectrometer collects semi-transmission spectrum data in tea extract, and the polyphenol content in the tea extract with different grades is obtained by combining with a conventional physicochemical analysis method; establishing a model for predicting the polyphenol content in the tea extract by combining the collected polyphenol content in the tea extract and semi-transmission spectrum data in the tea extract with a chemometrics method; based on the USB transmission technology, the quantitative analysis of the polyphenol content in the tea extract can be realized at the computer terminal.
Disclosure of Invention
The invention aims to provide a method and a device for rapidly detecting the polyphenol content in tea extract, which are used for rapidly and quantitatively analyzing the polyphenol content in the tea extract.
Aiming at the method and the device, the technical scheme is specifically adopted as follows:
a method for rapidly detecting the polyphenol content in tea extract is characterized by comprising the following steps:
step one, detecting the polyphenol content in tea extract: determining the polyphenol content in the tea extract of different grades by adopting a folin phenol reagent colorimetric method to obtain the polyphenol content in the tea extract determined by physicochemical analysis;
step two, collecting the spectral data of the tea extract: the method comprises the following steps of (1) adopting a light source module as a signal source, a semi-transmission detection device as a carrier of a sample, a micro optical fiber device as a transmission carrier of a spectrum signal, and a portable near infrared spectrum module to receive a semi-transmission spectrum in tea extracting solution, and carrying out rapid and nondestructive spectrum collection in the tea extracting solution to obtain semi-transmission near infrared spectrum data in the tea extracting solution;
step three, optimizing and analyzing a tea extracting solution detection model: preprocessing semi-transmission near infrared spectrum data in the tea extracting solution by adopting standard normal variable transformation (SNV), and establishing a tea extracting solution detection model by using the preprocessed near infrared spectrum data and the polyphenol content in the tea extracting solution determined by physical and chemical analysis;
step four, rapidly detecting the polyphenol content in the tea extract: the system is connected to a computer client through a USB data line, and transmits the near infrared spectrum data in the tea extracting solution in real time; combining a tea extract detection model written by development software, analyzing sample spectral information of the tea extract in real time at a software interface end, and realizing rapid detection of the polyphenol content in the tea extract; .
Further, in the second step, the light source module is provided with two halogen tungsten lamps; the working power of the halogen tungsten lamp is 1.4W, and the halogen tungsten lamp is placed at an included angle of 40 degrees horizontally; the portable near-infrared spectrometer is integrated into a cuboid of 10 x 6 x 5cm and can be carried by one hand; the fast speed is 2 s/time for scanning.
Further, in the second step, the wavelength range of the near infrared spectrum module is 900-1700 nm, so that the near infrared spectrum region can be effectively covered; the near infrared spectrum module has a slit size of 1.69 x 0.025mm, a signal-to-noise ratio of 6000:1 and high-throughput spectrum information; the near infrared spectrum module adopts an InGaAs detector, and has high sensitivity and detection rate.
Further, in the second step, in the semi-transmission detection device, spectrum collection is carried out in a semi-transmission mode; the semi-transmission mode is that only the optical signal of a single halogen tungsten lamp is received; the optical signals are gathered at the incident end interface through a special spherical lens.
Further, in the second step, in the micro optical fiber device, the optical signal is transmitted to the optical fiber exit end from the optical fiber exit port through the micro optical fiber; and the optical fiber emitting end emits the optical signal to the tea extracting solution in the cuvette clamping groove and semi-transmits the optical signal to the portable spectrometer to receive the semi-transmission signal.
Further, in the third step, the tea extract detection model is in a combination of four wavelength intervals of [1024.31nm1081.22nm ], [1311.08nm1362.62nm ], [1518.36nm1563.60nm ] and [1612.88nm1656.14nm ].
Further, in the fourth step, the software is written into a detection model supporting indexes of a plurality of internal components in the tea extracting solution; the tea extract detection model increases spectral information in a characteristic wavelength interval along with the increase of the number of collected samples, and the model can be optimized automatically; and (5) real-time analysis, namely verifying the stability of the spectral intensity, absorbance and transmittance of the tea extracting solution by adopting a mode of superimposed spectrum.
The technical scheme of the device provided by the invention is as follows:
a detection device for the polyphenol content in tea extract is characterized by comprising a semi-transmission detection device and a micro optical fiber device;
the semi-transmission detection device comprises an incident end interface, a light source emergent port, a cuvette clamping groove, a light source incident port and a screw hole; the front end of the device is provided with a light source emergent port, the rear end of the device is provided with a light source incident port and a screw hole, the upper end of the device is provided with a cuvette clamping groove, and one side of the device is provided with an incident end interface;
the semi-transmission detection device is fixed with the portable spectrometer through a screw hole; the light source signal is gathered in a spherical lens inside the device through a light source entrance port; the spherical lens is arranged in the entrance end interface; the incident end interface is communicated with an optical fiber incident end; the light source exit port is focused on the outer wall of the cuvette through the spherical lens; the cuvette is arranged in the cuvette clamping groove;
the micro optical fiber device comprises an optical fiber transmission port, an optical fiber incident end, a micro optical fiber and an optical fiber emergent end; an optical fiber transmission port is formed in the center of the optical fiber incident end; the optical fiber incident end and the optical fiber emergent end are connected through a micro optical fiber; and the optical fiber emergent end is connected with the light source emergent port.
Furthermore, in the semi-transmission detection device, the semi-transmission detection device is fastened to the portable spectrometer through a screw hole with the diameter of 2 x phi 2 mm; the diameter of the small ball lens is phi 10mm, and the focus of the small ball lens can be effectively placed at an optical fiber transmission port; the light source exit port is a standard SMA905 interface, and has better universality; the cuvette card slot specification is 12.5 × 45mm, and a 10mm quartz standard cuvette is suitable for use.
Furthermore, in the miniature optical fiber device, the optical fiber incident end is a copper cylinder with the bottom circle diameter phi of 10mm and the height of 10mm, so that the miniature optical fiber device is light in weight and easy to carry; the length of the micro optical fiber is 105mm, the micro optical fiber is connected to the optical fiber incident end and the optical fiber emergent end, and the center of the optical fiber incident end is provided with an optical fiber transmission port with phi 2 mm.
The invention has the following beneficial effects:
firstly, collecting tea extract spectral data: the invention adopts the semi-transmission spectrum detection device as a carrier for spectrum collection, adopts the micro optical fiber device as a transmission carrier for spectrum signals, combines the portable near-infrared spectrometer to collect semi-transmission spectrum data in the tea extract, has the advantages of rapidness, no damage, easy carrying and the like, and meets the requirements of on-site detection and analysis;
secondly, for the transmission of spectral data in the tea extract: the invention adopts USB transmission technology to transmit semi-transmission spectrum data, has stable signal and low noise, and is beneficial to the effective transmission of the spectrum data:
thirdly, rapidly detecting the polyphenol content in the tea extract: the method establishes a corresponding prediction model aiming at the polyphenol content in the tea extract, is different from the conventional physicochemical detection analysis, and has the advantages of simpler and more convenient rapid detection method and higher operability; the method supports the prediction of internal multi-components in the tea extracting solution, has a data storage function, and can improve the robustness of a polyphenol content model in the tea extracting solution at a later stage;
fourthly, optimizing and analyzing a polyphenol content detection model in the tea extracting solution: when a model for detecting the polyphenol content in the tea extract is combined with four wavelength ranges of [1024.31nm1081.22nm ], [1311.08nm1362.62nm ], [1518.36nm1563.60nm ], [1612.88nm1656.14nm ], the model correlation coefficient is higher, and the stability is better;
fifthly, the detection device for the polyphenol content in the tea extracting solution: the invention takes the semi-transmission detection device as a carrier for spectrum acquisition and the miniature optical fiber device as a transmission carrier for spectrum signals, combines the portable near-infrared spectrometer to acquire semi-transmission spectrum data in the tea extracting solution, can carry out rapid quantitative detection on the polyphenol content in the tea extracting solution, and is suitable for rapid detection and analysis on site.
Drawings
FIG. 1 is a schematic diagram of an apparatus of the present invention; FIG. 1(a) is a schematic view of a semi-transmissive detection device according to the present invention; FIG. 1(b) is a schematic view of a micro fiber device according to the present invention;
FIG. 2 is a schematic diagram of the optical path of the device for detecting the polyphenol content in the tea extract according to the present invention;
in the figure: the device comprises an incident end interface 1, a light source emergent port 2, a cuvette clamping groove 3, a light source incident port 4, a screw hole 5, an optical fiber transmission port 6, an optical fiber incident end 7, a miniature optical fiber 8 and an optical fiber emergent end 9;
Detailed Description
The technical solutions of the present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments. The invention has universality for quantitative detection of the internal components of the solution. The target polyphenol content selected in this embodiment is the main component in the tea extract, and other internal components of the solution can be taken with reference to this example.
In the embodiment, the semi-transmission detection device is used as a carrier for semi-transmission spectrum acquisition, and the micro optical fiber device is used as a transmission carrier for spectrum signals; and the semi-transmission spectrum signal is detected by combining a portable near-infrared spectrometer, so that the aim of rapid quantitative analysis is fulfilled. The specific technical scheme is as follows:
reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
Step one, detecting the polyphenol content in tea extract: determining the polyphenol content in the tea extract of different grades by adopting a folin phenol reagent colorimetric method to obtain the polyphenol content in the tea extract determined by physicochemical analysis;
step two, collecting the spectral data of the tea extract: the method comprises the following steps of (1) adopting a light source module as a signal source, a semi-transmission detection device as a carrier of a sample, a micro optical fiber device as a transmission carrier of a spectrum signal, and a portable near infrared spectrum module to receive a semi-transmission spectrum in tea extracting solution, and carrying out rapid and nondestructive spectrum collection in the tea extracting solution to obtain semi-transmission near infrared spectrum data in the tea extracting solution; in the second step, the light source module is provided with two halogen tungsten lamps; the working power of the halogen tungsten lamp is 1.4W, and the halogen tungsten lamp is placed at an included angle of 40 degrees horizontally; the portable near-infrared spectrometer is integrated into a cuboid of 10 x 6 x 5cm and can be carried by one hand; the fast speed is 2 s/time for scanning.
The wavelength range of the near infrared spectrum module is 900-1700 nm, and the near infrared spectrum region can be effectively covered; the near infrared spectrum module has a slit size of 1.69 x 0.025mm, a signal-to-noise ratio of 6000:1 and high-throughput spectrum information; the near infrared spectrum module adopts an InGaAs detector, and has high sensitivity and detection rate.
In the semi-transmission detection device, spectrum collection is carried out in a semi-transmission mode; the semi-transmission mode is that only the optical signal of a single halogen tungsten lamp is received; the optical signal is focused on the incident end interface 1 through a special spherical lens.
In the micro optical fiber device, optical signals are transmitted to an optical fiber outgoing end 9 from an optical fiber outgoing port 6 through a micro optical fiber 8; and the optical fiber emitting end 9 emits the optical signal to the tea extracting solution in the cuvette clamping groove 3 to be semi-transmitted to the portable spectrometer to receive the semi-transmission signal.
Step three, optimizing and analyzing a tea extracting solution detection model: preprocessing semi-transmission near infrared spectrum data in the tea extracting solution by adopting standard normal variable transformation (SNV), and establishing a tea extracting solution detection model by using the preprocessed near infrared spectrum data and the polyphenol content in the tea extracting solution determined by physical and chemical analysis; the tea extract detection model is combined with four wavelength intervals of [1024.31nm1081.22nm ], [1311.08nm1362.62nm ], [1518.36nm1563.60nm ], [1612.88nm1656.14nm ].
Step four, rapidly detecting the polyphenol content in the tea extract: the system is connected to a computer client through a USB data line, and transmits the near infrared spectrum data in the tea extracting solution in real time; and (3) combining a tea extracting solution detection model written in development software, analyzing the sample spectral information of the tea extracting solution in real time at a software interface end, and realizing the rapid detection of the polyphenol content in the tea extracting solution. The software is written into a detection model supporting indexes of a plurality of internal components in the tea extracting solution; the tea extract detection model increases spectral information in a characteristic wavelength interval along with the increase of the number of collected samples, and the model can be optimized automatically; and the real-time analysis verifies the stability of the spectral intensity, absorbance and transmittance of the tea extracting solution by adopting a mode of superimposed spectrum.
A detection device for polyphenol content in tea extract comprises a semi-transmission detection device and a micro optical fiber device;
the semi-transmission detection device comprises an incident end interface 1, a light source emergent port 2, a cuvette clamping groove 3, a light source incident port 4 and a screw hole 5; the front end of the device is provided with a light source emergent port 2, the rear end of the device is provided with a light source incident port 4 and a screw hole 5, the upper end of the device is provided with a cuvette clamping groove 3, and one side of the device is provided with an incident end interface 1;
the semi-transmission detection device is fixed with the portable spectrometer through a screw hole 5; the light source signal is gathered in a spherical lens inside the device through a light source entrance port 4; the spherical lens is arranged in the incident end interface 1; the incident end interface 1 is connected with an optical fiber incident end 7; the light source emergent port 2 is focused on the outer wall of the cuvette through a spherical lens; the cuvette is arranged in the cuvette clamping groove 3;
the micro optical fiber device comprises an optical fiber transmission port 6, an optical fiber incident end 7, a micro optical fiber 8 and an optical fiber emergent end 9; an optical fiber transmission port 6 is formed in the center of the optical fiber incident end 7; the optical fiber incident end 7 is connected with the optical fiber emergent end 9 through a miniature optical fiber 8; the optical fiber exit end 9 is connected with the light source exit port 2.
In the semi-transmission detection device, the semi-transmission detection device is fastened to the portable spectrometer through a screw hole with the diameter of 2 x phi 2 mm; the diameter of the small ball lens is phi 10mm, and the focus of the small ball lens can be effectively placed at the optical fiber transmission port 6; the light source emergent port 2 is a standard SMA905 interface and has better universality; the cuvette card slot specification is 12.5 × 45mm, and a 10mm quartz standard cuvette is suitable for use.
In the micro optical fiber device, the optical fiber incident end 7 is a copper cylinder with the bottom circle diameter phi of 10mm and the height of 10mm, so that the mass is light and the micro optical fiber device is easy to carry; the length of the micro optical fiber 8 is 105mm, the micro optical fiber is connected to the optical fiber incident end 7 and the optical fiber emergent end 9, and the center of the optical fiber incident end 7 is provided with an optical fiber transmission port 6 with phi 2 mm.
FIG. 1 is a schematic view of the semi-transmissive detection device of the present invention, referring to FIG. 1(a), the semi-transmissive detection device is fixed to the portable spectrometer through a screw hole; the light source signal is gathered on the ball lens through a light source incident port; the small ball lens is arranged in the incident end interface; the incident end interface is connected with an optical fiber incident end; the light source exit port is focused on the outer wall of the cuvette through a small spherical lens; the cuvette is arranged in the cuvette clamping groove;
FIG. 1(b) is a schematic view of a micro-fiber device of the present invention, in which a fiber transmission port is formed at the center of an incident end of a fiber; the optical fiber incident end and the optical fiber emergent end are connected through a micro optical fiber; and the optical fiber emergent end is connected with the light source emergent port.
The present invention will be described in detail with reference to specific embodiments. However, these embodiments are not intended to limit the present invention, and structural, methodological, or functional changes made by those skilled in the art according to these embodiments are included in the scope of the present invention.
The implementation case is as follows: detection of polyphenol content in tea extract of different grades
A novel method for rapidly detecting the polyphenol content in tea extract comprises the following steps:
(1) taking 60 tea extract samples with different grades, and detecting the polyphenol content in different samples by adopting a Fulin phenol reagent colorimetric method.
(2) Connecting the near-infrared spectrometer, the semi-transmission detection device and the micro optical fiber device according to the operation steps; three parallel test samples are respectively taken from each tea extract sample with different grades. The corresponding parameters of the spectrometer are set as follows: scanning wavelength range 900nm-1700nm, resolution 10.53, scanning point number 400, smoothness 5 point number, and average scanning frequency 5 times, and detecting near-infrared semi-transmission spectrum data.
(3) The collected near infrared spectrum contains noise information, background drift and the like, and standard normal variable transformation (SNV) is adopted to preprocess spectral data; screening variables by adopting a combined interval partial least square method (si-PLS), modeling spectral data, and quantitatively analyzing the polyphenol content in the tea extract. When the principal component is 9, the correlation coefficient reaches 0.965 and the RMSECV is 1.36 in the combination of the [ 381214 ] interval, so that the model stability is better. Table 1 shows the prediction result and the physicochemical analysis result of the polyphenol content in the tea extract based on the near infrared spectrum technology.
TABLE 1
| Sample numbering | Measured value (%) | Predicted value (%) | Error of the |
| 1 | 36.42 | 35.89 | -0.53 |
| 2 | 37.55 | 36.51 | -1.04 |
| 3 | 38.28 | 37.66 | -0.62 |
| 4 | 42.22 | 44.12 | 1.90 |
| 5 | 46.15 | 47.89 | 1.74 |
| 6 | 50.35 | 51.58 | 1.23 |
| 7 | 53.22 | 52.14 | -1.08 |
| 8 | 57.68 | 58.86 | 1.18 |
As shown in fig. 2, which is a schematic diagram of an optical path of fig. 1, the semi-transmission module: the halogen tungsten lamp is used as a light source signal, is focused on the input end of the miniature optical fiber through the spherical lens, transmits the light source signal to the output end of the optical fiber through the optical fiber, passes through the cuvette in a transmission mode, and transmits a transmission spectrum signal to the portable spectrometer.
A spectrometer: the transmission spectrum signal of the sample passes through the scanning slit, is calibrated by the collimating lens and points to the diffraction grating; then the light is irradiated onto the DLP micromirror corresponding to the light with the specific wavelength through the focusing lens; and finally, capturing the sample by an acquisition lens, directing the light to an InGaAs detector, and obtaining semi-transmission spectrum data of the sample on an ADC (analog-to-digital converter).
Transmission advantage:
DLP-based spectrometers replace conventional linear array detectors with Digital Micromirror (DMD) and single-point InGaAs detectors. By sequentially opening a set of columns of mirrors corresponding to light of a particular wavelength, the corresponding light is directed to a detector and captured. By scanning a set of mirror columns on the DMD, the transmission spectrum can be calculated.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an illustrative embodiment," "an example," "a specific example," or "some examples" or the like mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
While embodiments of the invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.
Claims (4)
1. A detection device for the polyphenol content in tea extract is characterized by comprising a semi-transmission detection device and a micro optical fiber device;
the semi-transmission detection device comprises an incident end interface (1), a light source emergent port (2), a cuvette clamping groove (3), a light source incident port (4) and a screw hole (5); the device is provided with a light source exit port (2) at the front end, a light source entrance port (4) and a screw hole (5) at the rear end, a cuvette clamping groove (3) at the upper end and an entrance end interface (1) at one side; in the semi-transmission detection device, the semi-transmission detection device is fastened to the portable spectrometer through a screw hole with the diameter of 2 x phi 2 mm; the diameter of the spherical lens is phi 10mm, and the focal point of the spherical lens can be effectively placed in the optical fiber transmission port (6); the light source emergent port (2) is a standard SMA905 interface and has better universality; the specification of the cuvette clamping groove is 12.5 × 45mm, and the cuvette clamping groove is suitable for a 10mm quartz standard cuvette;
the semi-transmission detection device is fixed with the portable spectrometer through a screw hole (5); the light source signal is gathered in a spherical lens inside the device through a light source entrance port (4); the spherical lens is arranged in the incident end interface (1); the incident end interface (1) is communicated with an optical fiber incident end (7); the light source emergent port (2) is focused on the outer wall of the cuvette through a spherical lens; the cuvette is arranged in the cuvette clamping groove (3);
the micro optical fiber device comprises an optical fiber transmission port (6), an optical fiber incident end (7), a micro optical fiber (8) and an optical fiber emergent end (9); an optical fiber transmission port (6) is formed in the center of the optical fiber incident end (7); the optical fiber incident end (7) is connected with the optical fiber emergent end (9) through a miniature optical fiber (8); the optical fiber exit end (9) is connected with the light source exit port (2).
2. The device for detecting the polyphenol content in the tea extracting solution according to claim 1, wherein in the micro optical fiber device, the optical fiber incident end (7) is a copper material cylinder with the bottom circle diameter phi of 10mm and the height of 10mm, so that the device is light in weight and easy to carry; the length of the miniature optical fiber (8) is 105mm, the miniature optical fiber is connected to the optical fiber incident end (7) and the optical fiber emergent end (9), and the center of the optical fiber incident end (7) is provided with an optical fiber transmission port (6) with phi 2 mm.
3. The method for rapidly detecting the polyphenol content in the tea extracting solution according to claim 1, which is characterized by comprising the following steps of:
step one, detecting the polyphenol content in tea extract: determining the polyphenol content in the tea extract of different grades by adopting a folin phenol reagent colorimetric method to obtain the polyphenol content in the tea extract determined by physicochemical analysis;
step two, collecting the spectral data of the tea extract: the method comprises the following steps of (1) adopting a light source module as a signal source, a semi-transmission detection device as a carrier of a sample, a micro optical fiber device as a transmission carrier of a spectrum signal, and a portable near infrared spectrum module to receive a semi-transmission spectrum in tea extracting solution, and carrying out rapid and nondestructive spectrum collection in the tea extracting solution to obtain semi-transmission near infrared spectrum data in the tea extracting solution;
in the micro optical fiber device, optical signals are transmitted to an optical fiber outgoing end (9) from an optical fiber outgoing port (6) through a micro optical fiber (8); the optical fiber emitting end (9) emits the optical signal to the tea extracting solution in the cuvette clamping groove (3) and semi-transmits the optical signal to the portable spectrometer to receive the semi-transmission signal; in the semi-transmission detection device, spectrum collection is carried out in a semi-transmission mode; the semi-transmission mode is that only the optical signal of a single halogen tungsten lamp is received; the optical signals are converged at an incident end interface (1) through a special spherical lens;
in the second step, the wavelength range of the near infrared spectrum module is 900-1700 nm, so that the near infrared spectrum region can be effectively covered; the near infrared spectrum module has a slit size of 1.69 x 0.025mm, a signal-to-noise ratio of 6000:1 and high-throughput spectrum information; the near infrared spectrum module adopts an InGaAs detector;
step three, optimizing and analyzing a tea extracting solution detection model: preprocessing semi-transmission near infrared spectrum data in the tea extracting solution by adopting standard normal variable transformation (SNV), and establishing a tea extracting solution detection model by using the preprocessed near infrared spectrum data and the polyphenol content in the tea extracting solution determined by physical and chemical analysis;
in the third step, the detection model of the tea extract is in four wavelength intervals of combination of [1024.31nm1081.22nm ], [1311.08nm1362.62nm ], [1518.36nm1563.60nm ] and [1612.88nm1656.14nm ];
step four, rapidly detecting the polyphenol content in the tea extract: the system is connected to a computer client through a USB data line, and transmits the near infrared spectrum data in the tea extracting solution in real time; combining a tea extract detection model written by development software, analyzing sample spectral information of the tea extract in real time at a software interface end, and realizing rapid detection of the polyphenol content in the tea extract;
the software is written into a detection model supporting indexes of a plurality of internal components in the tea extracting solution; the tea extract detection model increases spectral information in a characteristic wavelength interval along with the increase of the number of collected samples, and the model can be optimized automatically; and the real-time analysis verifies the stability of the spectral intensity, absorbance and transmittance of the tea extracting solution by adopting a mode of superimposed spectrum.
4. The rapid detection method according to claim 3, wherein in step two, the light source module has two halogen tungsten lamps; the working power of the halogen tungsten lamp is 1.4W, and the halogen tungsten lamp is placed at an included angle of 40 degrees horizontally; the portable near-infrared spectrometer is integrated into a cuboid of 10 x 6 x 5cm and can be carried by one hand; the fast speed is 2 s/time for scanning.
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| CN108241016B (en) * | 2018-02-01 | 2020-08-28 | 江苏大学 | Method and device for rapidly detecting theaflavin content in black tea |
| CN108572158A (en) * | 2018-07-11 | 2018-09-25 | 天津博霆光电技术有限公司 | A kind of household superminiature near infrared spectrum tester |
| CN109187454A (en) * | 2018-08-10 | 2019-01-11 | 江苏大学 | A kind of fluorescence detection method of tealeaves risk substance fluorine |
| CN110161025A (en) * | 2019-06-12 | 2019-08-23 | 郑州轻工业学院 | A kind of Continuous Flow Analysis method measuring tea polyphenols in tealeaves and tea product |
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Application publication date: 20170818 Assignee: NANJING RONGDIAN FOODSTUFF Co.,Ltd. Assignor: JIANGSU University Contract record no.: X2020980010180 Denomination of invention: A rapid detection method and device for polyphenol content in tea extract Granted publication date: 20200925 License type: Exclusive License Record date: 20201230 |