CN107045068A - Portable physiological Indexs measure instrument and its detection method based on micro-fluidic paper chip - Google Patents
Portable physiological Indexs measure instrument and its detection method based on micro-fluidic paper chip Download PDFInfo
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- CN107045068A CN107045068A CN201710243068.6A CN201710243068A CN107045068A CN 107045068 A CN107045068 A CN 107045068A CN 201710243068 A CN201710243068 A CN 201710243068A CN 107045068 A CN107045068 A CN 107045068A
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Abstract
The portable physiological Indexs measure instrument and its detection method based on micro-fluidic paper chip of the present invention, including tested physical signs sample, micro-fluidic paper chip, voltage follower module, voltage amplifier module, A/D modular converters, the one-chip computer module being sequentially connected, key-press module that one-chip computer module is connected respectively, display module, voice module connection, efficiently solve the problem of causing measurement error with micro-fluidic paper chip detection physical signs.Present inventive concept is ingenious, human oriented design and portability, uric acid concentration is detected using new micro-fluidic paper chip, the electric potential signal produced after biochemical reaction is digital quantity electric signal feeding STM32 series monolithics intellectual analysis and processing through AD conversion after voltage follow, voltage amplification, testing result can quickly be obtained and shown on screen, and system can point out the concentration to be higher relative to health standards, relatively low or normal measurement result is reliable, with market development potential.
Description
Technical field
The present invention relates to bioscience and engineering in medicine field, the portable physiological for being based particularly on micro-fluidic paper chip refers to
Mark detector and its detection method.
Background technology
Micro-fluidic refill chip technology is biology, chemistry, the sample preparation of medical analysis process, reaction, separation, detection etc.
Basic operation unit is integrated on the chip of one piece of micro-meter scale, is automatically performed the overall process of analysis, i.e., micro-fluidic paper chip is
The special electrochemica biological sensor of one class.
The general medium based on conductive ion of electrochemica biological sensor, electric current, electricity are converted into by targeted physiologic signal
Electric signal or the physical signallings such as position, because cheap, environment resistant interference performance is strong, easy to operate, electrochemica biological is detected such as
There is very big development prospect the present.
In the design of electrochemical sensor, current mode, conductivity type, electric potential type can be divided into according to the difference of measurement signal and passed
Sensor, because Copper diethlydithiocarbamate simply easily realizes that we select potentiometric measurement.
The general principle of potential measurement is that redox reaction can produce the transfer of electronics pair, so as to cause the electricity on electrode
Position is changed, and the concentration of reactant is measured by current potential size.General electrode all has ion selectivity, according to electrochemistry
In Nernst equation can just obtain corresponding concentration.
It was found from from nernst equation (2-1), it is temperature-resistant in the case of, the logarithm of electrode potential and reactant concentration is into line
Sexual intercourse, the graph of a relation of can make a response thing concentration and electrode potential realizes the purpose of measurement by comparing.
From the point of view of experimental result, although disclosure satisfy that use demand, but the chip made by hand still has precision problem,
This is due to that there are certain requirements on the storage time (in 30 days) of enzyme in chip, storage condition (2~4 DEG C of refrigerating chamber), also, often
Enzymatic activity there may be difference in one chip, cause measurement error.
Therefore, the present invention provides portable physiological Indexs measure instrument and its detection method based on micro-fluidic paper chip.
The content of the invention
For above-mentioned situation, to overcome the defect of prior art, the purpose of the present invention is just to provide based on micro-fluidic refill
The portable physiological Indexs measure instrument and its detection method of piece, efficiently solve and are led with micro-fluidic paper chip detection physical signs
The problem of causing measurement error.
Its technical scheme solved is that the portable physiological Indexs measure instrument and its detection method of micro-fluidic paper chip are wrapped
Include including tested physical signs sample, micro-fluidic paper chip, A/D modular converters, one-chip computer module, key-press module, display module,
Voice module, the tested physical signs sample, micro-fluidic paper chip, A/D modular converters, one-chip computer module are sequentially connected, single
Piece machine module is connected with key-press module, display module, voice module respectively, it is characterised in that micro-fluidic paper chip and A/D conversions
Voltage follower module, voltage amplifier module are connected between module, specially micro-fluidic paper chip connection voltage follower
Module, voltage follower module connection voltage amplifier module, voltage amplifier module connection A/D modular converters.
Portable physiological Indexs measure instrument and its detection method based on micro-fluidic paper chip, to be tested physical signs sample
One of uric acid exemplified by, specifically include following steps,
Step 1, physical signs sample is put into micro-fluidic paper chip, while micro-fluidic paper chip biochemical reaction starts, is pressed
Trigger switch key KEY3 in lower key-press module；
Step 2, on the basis of step 1, system electrification, single-chip microcomputer initial configuration, into the main interface of equipment:
" there is countdown to show below Welcometo Microcare Partner " signal languages, signal language；
Step 3, on the basis of step 2, during this period (this example is 30 seconds), user can be by the break key in key-press module
KEY1 stops countdown, and into next interface, user can check the electrode voltage and corresponding concentration at this moment；
Step 4, on the basis of step 2, if not pressing break key KEY1 keys, after 30 seconds, system automatically switches to next interface,
User can see the uric acid concentration detected in sample, and system can point out the concentration be relative to health standards it is higher,
It is relatively low or normal；
Step 5, on the basis of step 4, while uric acid concentration scope when providing normal is with for reference；
Step 6, return key KEY0 is pressed if to measure next group of sample on the basis of step 3,4,5 either steps, you can
Return to step 2 reclocking.
Present inventive concept is ingenious, human oriented design and portability, detects that uric acid is dense using new micro-fluidic paper chip
The analog voltage signal produced after degree, biochemical reaction is that digital quantity electric signal is sent through AD conversion after voltage follow, voltage amplification
Enter STM32 series monolithics intellectual analysis and processing, can quickly obtain testing result and be shown on screen, and system can be carried
It is higher relative to health standards to show the concentration, and relatively low or normal measurement result is reliable, with market development potential.
Brief description of the drawings
Fig. 1 is integrated circuit link block figure of the invention.
Fig. 2 is control method flow chart of the invention.
Fig. 3 is voltage follower modular circuit catenation principle figure of the invention.
Fig. 4 is voltage amplifier modular circuit catenation principle figure of the invention.
Fig. 5 closes curve matching figure for two electrode potentials of the present invention with uric acid concentration change.
Embodiment
For the present invention foregoing and other technology contents, feature and effect, in following cooperation with reference to figures 1 through Fig. 5 pairs
In the detailed description of embodiment, can clearly it present.The structure content being previously mentioned in following examples, is attached with specification
Figure is reference.
Each exemplary embodiment of the present invention is described below with reference to accompanying drawings.
Embodiment one, portable physiological Indexs measure instrument and its detection method based on micro-fluidic paper chip, including it is tested
Physical signs sample, can be blood glucose, uric acid etc., take appropriate amount to be put into micro-fluidic paper chip, micro-fluidic paper chip utilizes silk screen
The carbon electrode uric acid sensing chip of printing detects uric acid concentration, and it is by constantly improving, test, is cheap, conveniently
Quickly, precision is higher, portable easy-operating paper chip, and the front end tailored interfaces through micro-fluidic paper chip gather faint simulation electricity
Signal is pressed, this faint analog voltage signal enters row buffering, isolation into voltage follower module, improves load capacity, then passes through
Voltage amplifier module, carries out feeding A/D modular converters after in-phase proportion amplification and analog electrical signal is converted into digital electric signal,
One-chip computer module receives digital quantity electric signal and the operational order of key-press module user, and control display module intuitive interface is shown
(display detected value, prompting end value), voice module intelligent voice prompt operation sound and detected value, prompting end value.
Portable physiological Indexs measure instrument and its detection method based on micro-fluidic paper chip, to be tested physical signs sample
One of uric acid exemplified by, specifically include following steps,
Step 1, physical signs sample is put into micro-fluidic paper chip, while micro-fluidic paper chip biochemical reaction starts, is pressed
Trigger switch key KEY3 in lower key-press module；
Step 2, on the basis of step 1, system electrification, single-chip microcomputer initial configuration, into the main interface of equipment:
" there is countdown to show below Welcometo Microcare Partner " signal languages, signal language；
Step 3, on the basis of step 2, during this period (this example is 30 seconds), user can be by the break key in key-press module
KEY1 stops countdown, and into next interface, user can check the electrode voltage and corresponding concentration at this moment；
Step 4, on the basis of step 2, if not pressing break key KEY1 keys, after 30 seconds, system automatically switches to next interface,
User can see the uric acid concentration detected in sample, and system can point out the concentration be relative to health standards it is higher,
It is relatively low or normal；
Step 5, on the basis of step 4, while uric acid concentration scope when providing normal is with for reference；
Step 6, return key KEY0 is pressed if to measure next group of sample on the basis of step 3,4,5 either steps, you can
Return to step 2 reclocking.
Embodiment two, on the basis of embodiment one, the voltage follower module has been used to buffer, has isolated, improves band
The effect of loading capability, and then the degree of accuracy of measurement is improved, include model OPA656U operational amplifier U1, operational amplifier
U1 pin 3 is that in-phase input end connects the letter after the grounded resistance R1 weakenings of electrochemical signals produced by micro-fluidic paper chip
Number, because according to operational amplifier void is short, resolution, if the internal resistance of electrochemical signals is very big, that is, with carrying
Poor ability, the current signal of reflection will be formed about making an uproar as very strong transmitting concussion namely " echo " in input point
Sound will make quality of output signals very poor by amplification, and operational amplifier U1 pin 2 is directly connected to computing for in-phase input end
The amplifier U1 output end of pin 6, i.e. output voltage follow the amplitude and phase of input voltage completely, and multiplication factor is 1, though nothing
Voltage amplification factor, but have certain current output capability.Circuit serves impedance change action, lifts the bringing onto load energy of circuit
Power, a low impedance sources are converted into by a high impedance signal source.Attenuated signal input circuit high impedance and export back
Road is low-impedance to influence each other, and plays again to input, the isolation of input circuit and cushioning effect.Operational amplifier U1 pin 7
For positive power source terminal, power supply+5V is provided after electric capacity C2 and electrochemical capacitor C5 filtering clutters, operational amplifier U1 pin 4 is negative
Power end, provides power supply -5V after electric capacity C1 and electrochemical capacitor C6 filtering clutters.
Embodiment three, on the basis of embodiment one, the voltage amplifier module is used for voltage follower module is defeated
The voltage signal equal proportion amplification gone out is in feeding A/D modular converters, including model OPA8431D operational amplifier U2, and computing is put
The voltage signal that big device U2 pin 3 is exported for in-phase input end by resistance R3 concatenation operation amplifiers U1 pin 6, computing
Amplifier U2 pin 2 connects grounding resistance R2 one end, resistance R4 one end respectively, and the resistance R4 other end connects fortune respectively
Calculate amplifier U2 pin 6, resistance R3, resistance R4 composition feedback proportional coefficient, voltage amplification factor=1+R4/R2, this root
According to uric acid concentration and electric potential relation, it is respectively 46K Ω, 20K Ω, the voltage letter now amplified to set resistance R2, resistance R4 resistances
Number path input of the feeding using AD7606 chips as core A/D modular converters, operational amplifier U2 pin 7 is positive supply
End, provides power supply+5V, operational amplifier U2 pin 4 is negative power end after electric capacity C4 and electrochemical capacitor C7 filtering clutters, by
Power supply -5V is provided after electric capacity C3 and electrochemical capacitor C8 filtering clutters.
In the use of the present invention, being put into physical signs sample in micro-fluidic paper chip, micro-fluidic paper chip biochemical reaction is opened
While beginning, trigger switch key KEY3 in module, system electrification, single-chip microcomputer initial configuration, into the master of equipment are pushed button
Display screen in interface, display module:" there is countdown below Welcome to Microcare Partner " signal languages, signal language
It has been shown that, micro-fluidic paper chip biochemical reaction terminate after through voltage follower module buffering, isolation, improve load capacity again through etc. ratio
The path input of the A/D modular converters using AD7606 as core is sent into after example amplification, A D7606 conversion chips are greatly reduced defeated
Enter influence of the impedance to input signal, so as to improve measurement accuracy, analog electrical signal is converted to digital electric signal by A D7606,
It is connected by parallel interface with STM32 single-chip microcomputers in one-chip computer module, with standard urinary after STM32 single-chip microcomputers reading AD sampled datas
Acid concentration is analyzed with potential value, calculated, and detects whether that the button operation in key-press module is instructed, and this process time is
30S, if without the instruction of any button operation, STM32 single-chip microcomputer control voice modules intelligent voice prompt operation sound and detection in 30s
Display screen automatically switches to next interface in value, prompting end value, display module, and user can see what is detected in sample
Uric acid concentration, reference value and the concentration be relative to health standards it is higher, it is relatively low or normal；User can button mould in 30s
Break key KEY1 in block stops countdown, into next interface, and user can check the electrode voltage at this moment and right
The concentration answered；In 30s, if the sample mistake to be measured or to measure next group of sample, return key KEY0 is pressed, can be surveyed again
Amount.
Claims (7)
1. portable physiological Indexs measure instrument and its detection method based on micro-fluidic paper chip, including tested physical signs sample
Product, micro-fluidic paper chip, A/D modular converters, one-chip computer module, key-press module, display module, voice module, the tested life
Reason assay, micro-fluidic paper chip, A/D modular converters, one-chip computer module are sequentially connected, one-chip computer module respectively with button mould
Block, display module, voice module connection, it is characterised in that be connected between micro-fluidic paper chip and A/D modular converters voltage with
With device module, voltage amplifier module, specially micro-fluidic paper chip connection voltage follower module, voltage follower module connects
Connect voltage amplifier module, voltage amplifier module connection A/D modular converters.
2. portable physiological Indexs measure instrument and its detection method according to claim 1 based on micro-fluidic paper chip,
Characterized in that, the voltage follower module includes operational amplifier U1, operational amplifier U1 pin 3 connects miniflow respectively
Control the electrochemical signals of paper chip generation and grounding resistance R1 one end, the operational amplifier U1 concatenation operation amplifier of pin 2
U1 pin 6, operational amplifier the U1 connection of pin 7 power supply+5V, electric capacity C2 one end and electrochemical capacitor C5 positive pole connection electricity
Source+5V, electric capacity the C2 other end and electrochemical capacitor C5 negative pole connection ground, the operational amplifier U1 connection power supply -5V of pin 4,
Electric capacity C1 one end and electrochemical capacitor C6 positive pole connection ground, the electric capacity C1 other end and electrochemical capacitor C6 negative pole connection electricity
Source -5V.
3. portable physiological Indexs measure instrument and its detection method according to claim 1 based on micro-fluidic paper chip,
Characterized in that, the voltage amplifier module includes operational amplifier U2, operational amplifier U2 pin 3 is connected by resistance R3
Operational amplifier U1 pin 6 is connect, operational amplifier U2 pin 2 connects grounding resistance R2 one end, the one of resistance R4 respectively
End, the resistance R4 other end difference concatenation operation amplifier U2 pin 6, the path input of A/D modular converters, operation amplifier
Device the U2 connection of pin 7 power supply+5V, electric capacity C4 one end and electrochemical capacitor C7 positive pole connection power supply+5V, electric capacity C4's is another
End and electrochemical capacitor C7 negative pole connection ground, the operational amplifier U2 connection power supply -5V of pin 4, electric capacity C3 one end and electrolysis
Electric capacity C8 positive pole connection ground, the electric capacity C3 other end and electrochemical capacitor C8 negative pole connection ground.
4. portable physiological Indexs measure instrument and its detection method according to claim 1 based on micro-fluidic paper chip,
Characterized in that, A/D modular converters are using AD7606 chips as core, one-chip computer module is using STM32 series monolithics as core.
5. portable physiological Indexs measure instrument and its detection method according to claim 2 based on micro-fluidic paper chip,
Characterized in that, operational amplifier U1 models OPA656U.
6. portable physiological Indexs measure instrument and its detection method according to claim 3 based on micro-fluidic paper chip,
Characterized in that, operational amplifier U2 models OPA8431D.
7. portable physiological Indexs measure instrument and its detection method based on micro-fluidic paper chip, with tested physical signs sample it
Exemplified by one uric acid, following steps are specifically included,
Step 1, physical signs sample is put into micro-fluidic paper chip, while micro-fluidic paper chip biochemical reaction starts, is pressed
Trigger switch key KEY3 in lower key-press module；
Step 2, on the basis of step 1, system electrification, single-chip microcomputer initial configuration, into the main interface of equipment:
" there is countdown to show below Welcome to Microcare Partner " signal languages, signal language；
Step 3, on the basis of step 2, during this period（This example is 30 seconds）, user can be by the break key in key-press module
KEY1 stops countdown, and into next interface, user can check the electrode voltage and corresponding concentration at this moment；
Step 4, on the basis of step 2, if not pressing break key KEY1 keys, after 30 seconds, system automatically switches to next interface,
User can see the uric acid concentration detected in sample, and system can point out the concentration be relative to health standards it is higher,
It is relatively low or normal；
Step 5, on the basis of step 4, while uric acid concentration scope when providing normal is with for reference；
Step 6, return key KEY0 is pressed if to measure next group of sample on the basis of step 3,4,5 either steps, you can
Return to step 2 reclocking.
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| CN112798773A (en) * | 2020-12-27 | 2021-05-14 | 北京工业大学 | STM32 single chip microcomputer-based high-resolution microplate reader electric control system |
| CN115063943A (en) * | 2022-08-15 | 2022-09-16 | 芯翼信息科技(上海)有限公司 | Smoke sensor and smoke detection equipment based on low-power-consumption analog-to-digital converter |
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