CN107043701A - A kind of cell expansion system for improving cell inoculation dispersiveness - Google Patents
A kind of cell expansion system for improving cell inoculation dispersiveness Download PDFInfo
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- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
Embodiments of the invention provide a kind of cell expansion system for improving cell inoculation dispersiveness, are related to technical field of cell culture.Cell can be uniformly distributed, prevent cell contacts suppression.A kind of cell expansion system for improving cell inoculation dispersiveness, including nutrition liquid supplying device and incubator, cell reactor and motor are provided with the incubator, the nutrition liquid supplying device is connected with the cell reactor, so that nutrient input is delivered in the cell reactor, the motor is fixedly connected with the incubator, and the cell reactor is connected with the motor, and the motor can drive the cell reactor to rotate.The present invention is used for cell culture.
Description
Technical field
The present invention relates to technical field of cell culture, more particularly to a kind of cell amplification system for improving cell inoculation dispersiveness
System.
Background technology
Cell culture refers to all in vitro cultures, and its implication refers to take out tissue in driven thing or body, passes through machinery
Method or the method for enzymic digestion tissue is separated into unicellular, allow its specific conditions in vitro of physiological environment etc. in analogue body
Lower progress incubation culture, is allowed to survive and grows.In order that cell can smooth growth, often need detection cell cultivation process in
The growing environment of cell.
The mode of existing cell culture supplying nutrition liquid is generally：Cell is inoculated in reactor by pipeline, then
Nutrient solution is provided by conveyance conduit for cell or tissue to grow for it.This training method is compared cell being seeded in culture dish
In mode, the input of nutrient solution continually, the more conducively growth of cell.Therefore, in large batch of cell culture, gradually
Start to use.
But, the scheme being inoculated in cell in reactor of prior art, due to by Pipeline transport inoculating cell, carefully
Tended to collect in when born of the same parents are inoculated in cell reactor together, so, in follow-up cell cultivation process, between cell mutually
Often contact inhibition phenomenon occurs in contact.Contact inhibition, when referring to the cell of multicellular organism carrying out in vitro culture, disperses patch
The cell of wall growth once mutually converges contact, and then stops mobile and growth phenomenon.Once cells contacting occur suppresses existing
As the yield and effect of cell culture will be largely effected on.
The content of the invention
Embodiments of the invention provide a kind of cell expansion system for improving cell inoculation dispersiveness, cell can be made uniformly to divide
Cloth, and prevent cell contacts suppression.
To reach above-mentioned purpose, embodiments of the invention are adopted the following technical scheme that：
A kind of cell expansion system for improving cell inoculation dispersiveness, including nutrition liquid supplying device and incubator, it is described
Cell reactor and motor are provided with incubator, the nutrition liquid supplying device is connected with the cell reactor, will
Nutrient input is delivered in the cell reactor, and the motor is fixedly connected with the incubator, the cell reactor
It is connected with the motor, the motor can drive the cell reactor to rotate.
Further, the cell reactor is connected by fixed structure with the output shaft of the motor, described to drive
Dynamic motor can drive the fixed structure to rotate, to drive the cell reactor to rotate.
Further, the cell reactor is cylinder-like structure, one end of the cell reactor and the driving electricity
The output shaft connection of machine, the other end is rotatably connected with the incubator, and the motor can drive the cell reactor
Rotated around its center axis.
Further, the fixed structure includes first clamping plate and second clamping plate, the first clamping plate and second folder
Plate fastens and can grip the cell reactor, and the first clamping plate is provided with and is connected with the output shaft of the motor
Connecting shaft.
Further, the cell reactor is cylinder-like structure, and the fixed structure is by the cell reactor
Portion is fixed.
Preferably, the angle that the motor can drive the cell reactor to rotate is 0~180 degree.
Preferably, the motor is stepper motor.
Further, nutrition sap cavity and cell growth chamber, the nutrition are divided into by filter membrane in the cell reactor
Sap cavity offers nutrient input entrance and nutrient input outlet, the nutrient input entrance of the nutrition sap cavity and the nutrition sap cavity
Nutrient input outlet connected with the nutrition liquid supplying device, make the nutrition liquid supplying device and the nutrition sap cavity shape
Into nutrient solution circulation canal, nutrient solution circulating pump is provided with the nutrient solution circulation canal.
Further, the filter membrane is hollow fiber conduit, and the nutrition sap cavity is the inner space of the hollow fiber conduit,
The cell growth chamber is the exterior space of the hollow fiber conduit.
Further, the cell growth chamber is communicated with cell harvestor, and the cell growth chamber and the cell are collected
Pipeline between device is connected with cell delivery pump.
The cell expansion system provided in an embodiment of the present invention for improving cell inoculation dispersiveness, including nutrition liquid supplying device
And incubator, will for ensureing to be provided with cell reactor and motor in the environment temperature required for cell growth, incubator
Cell is inoculated in cell reactor, is connected by nutrition liquid supplying device with cell reactor, can deliver to nutrient input carefully
In born of the same parents' reactor, grown for cell.Motor is fixedly connected with incubator, and cell reactor is passed with the motor
Dynamic connection, motor can drive cell reactor to rotate, so, when cell is inoculated with, and drive cell anti-by motor
Answer device to rotate, inoculating cell can be made to be evenly distributed in cell reactor, and in cell cultivation process, pass through cell effect
The rotation of device, can disturb the nutrient solution and cell in cell reactor, cell is uniformly distributed in cell reactor, to prevent
Only cell contacts suppression.Meanwhile, the rotation of cell reactor can promote cell fully to be contacted with nutrient solution, more added with
Beneficial to the growth of cell.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the accompanying drawing used required in technology description to be briefly described.It should be evident that drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is inoculated with the structural representation of the cell expansion system of dispersiveness for the raising cell of the embodiment of the present invention；
Fig. 2 is inoculated with the cell reactor of dispersed cell expansion system around fixation for the cell that improves of the embodiment of the present invention
The structural representation that structure is rotated；
Fig. 3 is inoculated with the cell reactor of dispersed cell expansion system around wherein for the cell that improves of the embodiment of the present invention
The structural representation that heart axis is rotated；
Fig. 4 shows for the structure of the fixed structure of the cell expansion system of the raising cell inoculation dispersiveness of the embodiment of the present invention
It is intended to；
Fig. 5 is inoculated with the cell reactor of dispersed cell expansion system around one for the cell that improves of the embodiment of the present invention
Hold the structural representation rotated；
Fig. 6 is inoculated with the structure of the cell reactor of the cell expansion system of dispersiveness for the raising cell of the embodiment of the present invention
Schematic diagram.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
In the description of the invention, it is to be understood that term " " center ", " on ", " under ", "front", "rear", " left side ",
The orientation or position relationship of the instruction such as " right side ", " vertical ", " level ", " top ", " bottom ", " interior ", " outer " are based on shown in the drawings
Orientation or position relationship, are for only for ease of the description present invention and simplify description, rather than indicate or imply signified device or
Element must have specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.
In the description of the invention, it is necessary to illustrate, unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected, or be integrally connected；It is right
For one of ordinary skill in the art, the concrete meaning of above-mentioned term in the present invention can be understood with concrete condition.
Embodiments of the invention provide a kind of cell expansion system for improving cell inoculation dispersiveness, as shown in Figure 1, Figure 2 and Fig. 3
It is shown, including nutrition liquid supplying device 1 and incubator 2, incubator 2 is interior to be provided with cell reactor 3 and motor 4 (Fig. 1 and figure
Not shown in 2), nutrition liquid supplying device 1 is connected with cell reactor 3, and nutrient input is delivered in cell reactor 3, is driven
Dynamic motor 4 is fixedly connected with incubator 2, and cell reactor 3 is connected with motor 4, and motor 4 can drive cell anti-
Device 3 is answered to rotate.
The cell expansion system provided in an embodiment of the present invention for improving cell inoculation dispersiveness, including nutrition liquid supplying device
1 and incubator 2, for ensureing in the environment temperature required for cell growth, incubator 2 provided with cell reactor 3 and driving electricity
Machine 4, cell is inoculated in cell reactor 3, is connected by nutrition liquid supplying device 1 with cell reactor 3, can be by nutrient solution
It is delivered in cell reactor 3, is grown for cell.Motor 4 is fixedly connected with incubator 2, and cell reactor 3 is with driving
Dynamic motor 4 is connected, and motor 4 can drive cell reactor 3 to rotate, so, when cell is inoculated with, pass through motor
4 driving cell reactors 3 are rotated, and inoculating cell can be made to be evenly distributed in cell reactor 3, and in cell cultivation process
In, by the rotation of cell reactor 3, the nutrient solution and cell in cell reactor 3 can be disturbed, makes cell in cell effect
It is uniformly distributed in device 3, to prevent cell contacts suppression.Meanwhile, the rotation of cell reactor 3 can promote cell and battalion
Nutrient solution is fully contacted, advantageously in the growth of cell.
It should be noted that nutrition liquid supplying device 1 only needs to ensure the normal supply of nutrient solution, such as such as Fig. 1
It is shown for standing reservoir bag 11, standing reservoir bag 11 is connected with cell reactor 3, with supplying nutrition liquid.Certainly, in order to
The temperature of nutrient solution is buffered, surge flask can also be increased, in order in advance mix gas with nutrient solution, to adjust the acid of nutrient solution
Basicity and dissolved oxygen amount, can also increase gas dissolver etc., not extend herein.
Embodiment one
As shown in Fig. 2 cell reactor 3 is connected by fixed structure 6 and the output shaft of the (not shown) of motor 4
Connect, motor 4 can drive fixed structure 6 to rotate, to drive cell reactor 3 to rotate.Cell grows in cell reactor 3
During, by motor 4 fixed structure 6 can be driven to rotate, to drive cell reactor 3 to rotate, cell can be disturbed anti-
The nutrient solution and cell in device 3 are answered, cell is uniformly distributed in reactor, to prevent cell contacts suppression.Together
When, the rotation of cell reactor 3 can promote cell fully to be contacted with nutrient solution, advantageously in the growth of cell.
It should be noted that motor 4 not shown in Fig. 2, motor 4 is mountable to fixed structure 6 and incubator 2
Between wall, the back side of incubator 2 can also be arranged on.
Fixed structure 6 only needs to fix cell reactor 3, then is connected by fixed structure 6 with motor 4, you can
Realize that the driving cell reactor 3 of motor 4 is rotated.As shown in figure 4, fixed structure 6 includes first clamping plate 61 and second clamping plate
62, first clamping plate 61 and second clamping plate 62 are fastened and can grip cell reactor 3, and first clamping plate 61 is provided with and driving electricity
The connecting shaft 63 of the output shaft connection of machine 4.
For the rotation of the growth of adaptation cell, and cell reactor 3, cell reactor 3 can be cylinder-like structure.Driving
The driving fixed structure 6 of motor 4 is rotated with cell reactor 3, i.e. the position of the fixation of fixed structure 6 cell reactor 3 is determined
The rotary motion trace of cell reactor 3.Because cell reactor 3 is cylinder-like structure, the rotation of cell reactor 3 can be such as figure
Being rotated around its middle part shown in 2 or the position around its one end as shown in Figure 5 are rotated.For comparing, cell effect
In the scheme that device 3 is rotated around its middle part, two parts rotate up and down in cell reactor 3 angle and speed almost symmetry, i.e. right
The disturbance of cell and nutrient solution in cell reactor 3 is more balanced.In addition, fixed structure 6 is used as fixed cell reactor 3
The strong point, two parts keep balancing to the centrifugal force of fixed structure 6 above and below it when cell reactor 3 is rotated, and consolidate fixed structure 6
It is scheduled on better reliability during 3 middle part of cell reactor.Therefore, reference picture 2, cell reactor 3 is cylinder-like structure, fixed knot
Structure 6 fixes the middle part of cell reactor 3, so, and motor 4 can drive cell reactor 3 to be rotated around its middle part.
Because cell reactor 3 needs connection nutrition liquid supplying device 1, to convey nutrient solution, generally, the conveying of nutrient solution
Connection flexible pipe is needed to use, so, the cell reactor 3 for being connected with flexible pipe is limited by flexible pipe, can only be rotated a certain angle.
By actual verification, motor 4 can drive cell reactor 3 to be 0~180 degree around the angle that fixed structure 6 is rotated, in not shadow
Ring on cell reactor 3 under the premise of the mechanical property of connection flexible pipe, it is ensured that to the inner cell of cell reactor 3 and nutrient solution
Disturbance, prevents cells contacting suppression.
Because the rotatable angle of cell reactor 3 is preferably 0~180 degree, it is therefore preferable that motor is stepping electricity
Machine.Stepper motor can be very good to meet the 0~180 degree of rotation of cell reactor 3, and stable, may be programmed, adjustable speed.
General, during cell culture, nutrient solution is passed through in cell culture apparatus, through that after a period of time, will give up
Liquid is discharged, and adds fresh nutrient solution.But, this mode causes the waste of nutrient solution, and is unfavorable for keeping cell life
The stabilization of dis environment.Therefore, as shown in fig. 6, being divided into nutrition sap cavity 32 and cell growth by filter membrane 31 in cell reactor 3
Chamber 33, nutrition sap cavity 32 offers nutrient input entrance 321 and nutrient input outlet 322, the nutrient infusion of nutrition sap cavity 32
Mouthfuls 321 and the nutrient input outlet 322 of nutrition sap cavity 32 connected with nutrition liquid supplying device 1, make nutrition liquid supplying device 1 with
Nutrient solution circulating pump 5 is provided with the formation nutrient solution circulation canal of nutrition sap cavity 32, nutrient solution circulation canal.So, in nutrition
In the presence of liquid circulating pump 5, nutrient solution is in nutrient solution circulation canal internal circulation flow.Filter membrane 31 can allow in nutrition sap cavity 32
Nutrient solution passes through, and prevents the cell in cell growth chamber 33 from passing through, and then the more fresh nutrient solution in nutrition sap cavity 32
Nutrition can be carried out with the nutrient solution in cell growth chamber 33 to exchange, fresh Nutrient is provided for cell.Nutrition in nutrition sap cavity 32
After liquid is exchanged with the nutrient solution progress part in cell growth chamber 33, continue in nutrient solution circulation canal internal circulation flow, take out of
The waste gas that cell growth is produced, dissolves in fresh oxygen, and then recycles nutrient solution, saves resource, can keep cells survival ring
The stabilization in border.
It should be noted that the nutrient solution in nutrition sap cavity 32 can be swapped with the nutrient solution in cell growth chamber 33,
It is to be carried out based on the pressure differential in nutrition sap cavity 32 and cell growth chamber 33, i.e. the nutrient solution in cell growth chamber 33
When not enough, the pressure in nutrition sap cavity 32 is more than the pressure in cell growth chamber 33, now, and the nutrient solution in nutrition sap cavity 32 leads to
Filter membrane 31 enters in cell growth chamber 33, for cell growth；When the cell quantity in cell growth chamber 33 increases, and new old generation
When thanking to thing aggregation, the pressure in nutrition sap cavity 32 is less than the pressure in cell growth chamber 33, now, thin in cell growth chamber 33
Born of the same parents' metabolism thing and outmoded nutrient solution are entered in nutrition sap cavity 32 by filter membrane 31, are then arranged by nutrient solution circulation canal
Go out or recycle.Therefore, during cell growth, the rotation of the driving cell reactor 3 of motor 4 not only can be to prevent
The phenomenon that only cells contacting suppresses, can also adjust the pressure differential of nutrition sap cavity 32 and cell growth chamber 33, and then accelerate nutrition
The nutrient solution of sap cavity 32 and cell growth chamber 33 is exchanged, and more promotes the growth of cell.
Reference picture 6, filter membrane 31 can be hollow fiber conduit, must because nutrient solution constantly flows in nutrient solution circulation canal
Larger impact is so produced to filter membrane 31, and hollow fiber conduit has the characteristic of self-supporting, can guarantee that nutrient solution enters with cell
On the premise of exchange is supported at field headquarters, ability is well adapted to because the impact of flowing generation has to nutrient solution.Therefore, filter membrane 31 is adopted
Use hollow fiber conduit.After hollow fiber conduit is employed, nutrition sap cavity 32 and cell growth chamber 33 can flexibly be set as needed
Put, for example, nutrition sap cavity 32 is the inner space of hollow fiber conduit, cell growth chamber 33 is the exterior space of hollow fiber conduit.
Or, nutrition sap cavity 32 is the exterior space of hollow fiber conduit, and cell growth chamber 33 is the inner space of hollow fiber conduit.
It should be noted that hollow fiber conduit can be a hollow fiber conduit or many hollow fiber conduits.Its
In, the interior or exterior space of many hollow fiber conduits compares the interior or exterior space of single hollow fiber conduit, and surface area is bigger, namely nutrient solution
Contact surface area it is bigger, and then nutrient solution in nutrition sap cavity 32 can fully connect with the cell in cell growth chamber 33
Touch, the exchange of nutrition is more efficient, accordingly, it is preferred that setting many hollow fiber conduits.
It is general, it is necessary to cell culture apparatus is removed, it is necessary to which cell is collected after cell culture terminates, then will be thin
Born of the same parents are collected into the container specified.But this manually-operated scheme is not enough facilitated, and pollution easily is produced to cell.Therefore,
As shown in figure 1, cell growth chamber 33 is communicated with cell harvestor 7, the pipeline between cell growth chamber 33 and cell harvestor 7 connects
It is connected to cell delivery pump 8.So, can be very easily defeated by cell by opening cell delivery pump 8 when needing to collect cell
Deliver in cell harvestor 7, it is to avoid the pollution that artificial operation may be brought, and easy to operate, collection efficiency is high.Meanwhile, carefully
The control that born of the same parents' delivery pump 8 passes through rotating, it is possible to achieve cell is is collected in a direction, and another direction is inoculating cell.
It should be noted that because the inwall that cell is easily gathered in cell growth chamber 33 during growth (works as nutrition
Sap cavity 32 is the inner space of hollow fiber conduit, and cell growth chamber 33 is the exterior space of hollow fiber conduit, and cell is easily gathered in
The outer wall of hollow fiber conduit；When the exterior space that nutrition sap cavity 32 is hollow fiber conduit, cell growth chamber 33 is hollow fiber conduit
Inner space, cell is easily gathered in the inwall of hollow fiber conduit), may collect cell not complete enough, in cell reactor 3
It is interior to produce certain residual.Therefore, when cell collects preceding and collection, it is necessary to take off the inwall of cell and cell growth chamber 33
From, by motor 4 cell reactor 3 can be driven to rotate, be uniformly distributed the cell for being gathered in the inwall of cell growth chamber 33,
And then the inwall of cell and cell growth chamber 33 departs from, and is easy to cell to collect, and reduces cell residue.Certainly, for suspension cell
Disengaging can be directly rotated, and attached cell needs first enzyme-added reaction to be rotated further by departing from.
Embodiment two
As shown in figure 3, the present embodiment and the difference of embodiment one are：Cell reactor 3 is cylinder-like structure, cell effect
One end of device 3 is connected with the output shaft of motor 4, and the other end is rotatably connected with incubator 2, and motor 4 can drive carefully
Born of the same parents' reactor 3 is rotated around its center axis.During cell grows in cell reactor 3, driven carefully by motor 4
Born of the same parents' reactor 3 is rotated around its center axis, can be disturbed the nutrient solution and cell in cell reactor 3, be made cell in reactor
It is uniformly distributed, to prevent cell contacts suppression.In addition, when cell is inoculated with, the driving cell reactor of motor 4
3 are rotated around its center axis, and inoculating cell can be made to be dispersed in cell reactor 3, promote cell and nutrient solution abundant
Contact, is easy to the growth of cell.
Likewise, the rotatable angle of cell reactor 3 is preferably 0~180 degree, motor 4 is preferably stepper motor；
Nutrition sap cavity 32 and cell growth chamber 33, nutrition liquid supplying device 1 and nutrient solution are divided into by filter membrane 31 in cell reactor 3
Nutrient solution circulating pump 5, the driving cell of motor 4 are provided with the formation nutrient solution circulation canal of chamber 32, nutrient solution circulation canal
Reactor 3 is rotated around its center axis, can adjust the pressure differential of nutrition sap cavity 32 and cell growth chamber 33, and then accelerate nutrient solution
The nutrient solution of chamber 32 and cell growth chamber 33 is exchanged, and more promotes the growth of cell.
It should be noted that cell reactor 3 is rotated around its center axis, to improve adjustment nutrition sap cavity 32 and cell life
The effect of the pressure differential of long chamber 33, direction of the filter membrane 31 along the centerline axis parallel of cell reactor 3 is set, so, cell effect
The centrifugal force that the rotational band of device 3 is come is vertical with filter membrane 31, advantageously in the nutrient solution in nutrition sap cavity 32 and cell growth chamber 33
The exchange occurred by filter membrane 31.
The embodiment of the present invention is these are only, but protection scope of the present invention is not limited thereto, any skill
The technical staff in art field the invention discloses technical scope in, the change or replacement that can be readily occurred in, should all cover this
Within the protection domain of invention.Therefore, protection scope of the present invention should be defined by scope of the claims.
Claims (10)
1. a kind of cell expansion system for improving cell inoculation dispersiveness, it is characterised in that including nutrition liquid supplying device and training
Support and cell reactor and motor are provided with case, the incubator, the nutrition liquid supplying device and the cell reactor
Connection, nutrient input is delivered in the cell reactor, the motor is fixedly connected with the incubator, described thin
Born of the same parents' reactor is connected with the motor, and the motor can drive the cell reactor to rotate.
2. cell expansion system according to claim 1, it is characterised in that the cell reactor by fixed structure with
The output shaft connection of the motor, the motor can drive the fixed structure to rotate, to drive the cell anti-
Device is answered to rotate.
3. cell expansion system according to claim 1, it is characterised in that the cell reactor is cylinder-like structure,
One end of the cell reactor is connected with the output shaft of the motor, and the other end is rotatably connected with the incubator,
The motor can drive the cell reactor to be rotated around its center axis.
4. cell expansion system according to claim 2, it is characterised in that the fixed structure includes first clamping plate and the
Second splint, the first clamping plate and the second clamping plate fasten and can grip the cell reactor, the first clamping plate
Provided with the connecting shaft being connected with the output shaft of the motor.
5. cell expansion system according to claim 2, it is characterised in that the cell reactor is cylinder-like structure,
The fixed structure fixes the middle part of the cell reactor.
6. the cell expansion system according to Claims 2 or 3, it is characterised in that the motor can drive described thin
The angle that born of the same parents' reactor is rotated is 0~180 degree.
7. the cell expansion system according to Claims 2 or 3, it is characterised in that the motor is stepper motor.
8. the cell expansion system according to Claims 2 or 3, it is characterised in that pass through filter membrane in the cell reactor
Nutrition sap cavity and cell growth chamber are divided into, the nutrition sap cavity offers nutrient input entrance and nutrient input outlet, described
The nutrient input outlet of the nutrient input entrance of nutrition sap cavity and the nutrition sap cavity is connected with the nutrition liquid supplying device,
Make to set in the nutrition liquid supplying device and nutrition sap cavity formation nutrient solution circulation canal, the nutrient solution circulation canal
Nutritious liquid circulating pump.
9. cell expansion system according to claim 8, it is characterised in that the filter membrane is hollow fiber conduit, the battalion
Nutrient solution chamber is the inner space of the hollow fiber conduit, and the cell growth chamber is the exterior space of the hollow fiber conduit.
10. cell expansion system according to claim 8, it is characterised in that the cell growth chamber is communicated with cell receipts
Storage, the pipeline between the cell growth chamber and the cell harvestor is connected with cell delivery pump.
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Cited By (5)
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| CN109825434A (en) * | 2019-02-01 | 2019-05-31 | 赵涌 | Cell bionic intelligence production system |
| WO2019120135A1 (en) * | 2017-12-18 | 2019-06-27 | 上海白泽医疗器械有限公司 | Cell culture module and cell culture system |
| WO2019219050A1 (en) * | 2018-05-18 | 2019-11-21 | Huacells Corporation | Automated cell culturing |
| CN113105999A (en) * | 2021-03-02 | 2021-07-13 | 中国人民解放军陆军军医大学第二附属医院 | Subcutaneous tumor cell inoculation device capable of improving dispersity and using method |
| US20220017850A1 (en) * | 2015-02-09 | 2022-01-20 | Univercells Technologies Sa | System, apparatus and method for the production of cells and/or cell products |
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| CN206014953U (en) * | 2016-09-01 | 2017-03-15 | 奥凯（苏州）生物技术有限公司 | A kind of cell culture apparatuses of nutritional solution dynamic circulation |
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| US20220017850A1 (en) * | 2015-02-09 | 2022-01-20 | Univercells Technologies Sa | System, apparatus and method for the production of cells and/or cell products |
| WO2019120135A1 (en) * | 2017-12-18 | 2019-06-27 | 上海白泽医疗器械有限公司 | Cell culture module and cell culture system |
| WO2019219050A1 (en) * | 2018-05-18 | 2019-11-21 | Huacells Corporation | Automated cell culturing |
| TWI765154B (en) * | 2018-05-18 | 2022-05-21 | 美商華賽公司 | Automative cell culturing system and method for culturing cells |
| CN109825434A (en) * | 2019-02-01 | 2019-05-31 | 赵涌 | Cell bionic intelligence production system |
| CN109825434B (en) * | 2019-02-01 | 2020-07-10 | 赵涌 | Cell bionic intelligent production system |
| CN113105999A (en) * | 2021-03-02 | 2021-07-13 | 中国人民解放军陆军军医大学第二附属医院 | Subcutaneous tumor cell inoculation device capable of improving dispersity and using method |
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