CN107034123A - Agricultural ATCSP Internet of Things spore information catches culture systems automatically - Google Patents
Agricultural ATCSP Internet of Things spore information catches culture systems automatically Download PDFInfo
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- CN107034123A CN107034123A CN201710198438.9A CN201710198438A CN107034123A CN 107034123 A CN107034123 A CN 107034123A CN 201710198438 A CN201710198438 A CN 201710198438A CN 107034123 A CN107034123 A CN 107034123A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
Culture systems are caught automatically the present invention relates to a kind of agricultural ATCSP Internet of Things spore information.The system includes, by motor-driven rotating disk, carrying glass box, blast pipe, blower fan, by motor-driven ejecting device, and control computer；Rotating disk, ejecting device and blower fan are controlled to run by control computer；Glass box, blast pipe and ejecting device is carried to be fixed on above rotating disk；When the groove that rotating disk is provided with least one groove, rotating disk is turned to below load glass box, the slide carried in glass box is fallen into groove；The groove equipped with slide is rotated further to blast pipe with rotating disk afterwards, under fan action in the distinguished and admirable entrance blast pipe comprising spore, and gathers spore by the adhesive on slide；Slide is released into groove by ejecting device afterwards.The system can round-the-clock unattended continuous automatic work, and can network with Internet of things system, realize country, province, city, county, township information gathering stations at different levels are wirelessly transferred, remote control and information data are shared.
Description
Technical field
The invention belongs to plant disease prevention forecasting technique field in agriculture and forestry production, it is related to spore trap and analytical technology, has
Body is related to a kind of spore of Internet of Things and catches culture systems automatically.
Background technology
Aeroborne disease is generally the main prevention and control object in agriculture and forestry production, and wherein powdery mildew, downy mildew, rust etc. is emphasis
Prevention and control object, is included in national plant disease prevention forecast project, substantial amounts of manpower and materials is needed every year to complete the prediction of related disease
Forecast.And with air spore trap instrument (the air spore trap instrument developed such as Hebi Jia Duo science, industry and trades limited company)
Success, hence it is evident that alleviate the workload that plant protection personnel predict disease.
But in prediction disease field, however it remains grass-roots work personnel will not analyze and identify Spores, information transfer is slow,
Data sharing not in time the problems such as, it is impossible to the need for meeting the modern disease prediction technology that becomes more and more important.
The content of the invention
In view of the shortcomings of the prior art, the present invention proposes that a kind of spore catches culture and statistical analysis system automatically, can
The information gathering of spore is realized in field, realizes that the identification of cause of disease and plant disease prevention are pre- (on the computer in such as office) indoors
Report.The program reduces the workload and pollution condition that staff collects spore slide to field, realize it is round-the-clock nobody
Continuously and automatically spore field seizure on duty, culture on the spot, micro-imaging, real-time Data Transmission, real time information are shared, Zhuan Jiafen
Analysis etc., science, the information gathering and identification for reasonably realizing spore the need for meeting plant disease prevention forecasting technique, are saved
Substantial amounts of manpower and materials.
System is caught automatically present invention firstly provides a kind of spore, including one by motor-driven rotating disk, one equipped with carrying glass
The load glass box of piece, a blast pipe, a blower fan, one by motor-driven ejecting device, and a control computer；Rotating disk, release
Device and blower fan are controlled to run by control computer；Glass box, blast pipe and ejecting device is carried to be fixed on above rotating disk；Set on rotating disk
When having the groove at least one groove, rotating disk to turn to below load glass box, the slide carried in glass box is fallen into groove；Afterwards
Groove equipped with slide is rotated further to blast pipe with rotating disk, and the distinguished and admirable entrance air intake of spore is included under fan action
In pipe, and spore is gathered by the adhesive on slide；Slide is released into groove by ejecting device afterwards.
Further, the groove is at least two, and the load glass box is built with multi-disc slide；First slide falls
Enter in the first groove and turn to below blast pipe to gather during spore, the second groove, which is located at, carries below glass box and fall into second
Slide, afterwards second slide turn to below blast pipe to gather spore；Slide after collection spore is pushed out dress
Put after release groove, the groove is rotated further to load glass box and falls into the 3rd slide；So realize lasting collection.
The present invention also provide a kind of spore catch automatically, culture systems, on the basis of said apparatus, in addition to culture dress
Put, it is used to extruding the pressurizing unit of infusion bag comprising infusion bag, woven hose and by motor-driven, the infusion bag built with
Nutrient solution, the pressurizing unit is controlled to run by the control computer；Slide after collection spore is rotated further with rotating disk
To the culture apparatus, under the control of the control computer and the driving of motor, infusion bag is extruded by pressurizing unit, made
Nutrient solution is added on slide at regular time and quantity by woven hose, realizes automatic liquid-feeding culture；Glass will be carried by ejecting device afterwards
Piece releases groove.
Further, the groove is at least three, and the load glass box is built with multi-disc slide；First slide falls
Enter in the first groove and turn to below blast pipe to gather during spore, the second groove, which is located at, carries below glass box and fall into second
Slide；When first slide after collection spore is rotated further to the woven hose of culture apparatus to be cultivated, the
Two slides turn to below blast pipe to gather spore, while the 3rd groove, which is located at, carries below glass box and fall into the 3rd load
Slide；So realize lasting collection and culture.
The present invention also provide a kind of spore catch, cultivate automatically, imaging system, on the basis of said apparatus, in addition into
As device, it includes microscope and video camera, and wherein video camera is driven by motor, and controls operation by the control computer；
Spore after culture is turned under the imaging device, drives video camera to be taken pictures by motor after amplifying through microscope；It
Slide is released into groove by ejecting device afterwards.
Further, the groove is at least four, and the load glass box is built with multi-disc slide；First slide falls
Enter in the first groove and turn to below blast pipe to gather during spore, the second groove, which is located at, carries below glass box and fall into second
Slide；When first slide after collection spore is rotated further to the woven hose of culture apparatus to be cultivated, the
Two slides turn to below blast pipe to gather spore, while the 3rd groove, which is located at, carries below glass box and fall into the 3rd load
Slide；When first slide after being cultivated is rotated further to imaging device, second slide is located at culture and filled
To be cultivated below the woven hose put, while the 3rd groove is located at below blast pipe to gather spore, while the 4th groove in
Carry below glass box and fall into the 4th slide；So realize lasting collection, culture and be imaged.
Further, the imaging device includes transport module, for by wired or wireless way by the spore of shooting
Image transmitting is to Internet of Things server；The Internet of Things server is handled spore image, including carries out automatically analyzing sieve
Choosing.
Further, in addition to an externally-located casing, the casing includes a nor'wester and a three-dimensional bracket.
The spore information of the present invention catch automatically culture systems using air-flow is quantitative, timing acquiring it is (unified blower fan model, logical
Wind pipeline diameter, it has been determined that air-flow per minute is the same, is that control computer controls exhausting time consistency by controller,
Reach that air-flow is quantified；Controller setting acquisition time timing acquiring spore number of times), automatic culture (can be for the setting of different spores
Different incubation times), automated imaging, long range radio transmissions, display in real time, storage germ spore image；Believed by germ image
Storehouse is ceased, the change of the Spores quantity in field is analyzed, time of origin, occurrence degree and the round of disease is predicted, is spore
The special equipment of son monitoring, being capable of round-the-clock unattended continuous automatic work.The system can network with Internet of things system, such as with
(ATCSP is the Internet of things system for the agricultural ATCSP Internet of things system networking of Hebi Jia Duo science, industry and trades limited company exploitation
Code name), reach country, province, city, county, township information gathering stations at different levels be wirelessly transferred, remote control and information data it is shared.
Brief description of the drawings
Fig. 1 is the structural representation that spore catches culture systems automatically.
Fig. 2A~Fig. 2 D are the schematic diagram of inner cylinder 200, and wherein Fig. 2A is that inner cylinder removes the solid after the barrel of top half
Structural representation, Fig. 2 B are to remove the dimensional structure diagram after whole barrels, and Fig. 2 C are Fig. 2 B top view, and Fig. 2 D are Fig. 2 C
The sectional view in middle C-C sections.
The schematic diagram of grooves of the Fig. 3 for rotating disk and its above.
Fig. 4 A~Fig. 4 C are the schematic diagram of extruding type micro automatic liquid feeder, and wherein Fig. 4 A, Fig. 4 C are stereogram, Fig. 4 B
For top view.
Fig. 5 is the structure chart of imaging device.
Fig. 6 is the structure chart of ejecting device.
Fig. 7 is the schematic diagram that ejecting device releases slide.
Fig. 8 is the polycrystalline substance schematic diagram of inner cylinder.
Fig. 9 catches the flow chart of culture and statistical analysis for progress spore information automatically.
Figure 10~Figure 12 is disease occurrence degree and the Linear correlative analysis figure of the germ quantitative relation of capture.Wherein Figure 10
Sporangium magnitude relation was captured for disease occurrence degree and the same day, Figure 11 is that disease occurrence degree captured sporangium and spore with the same day
Magnitude relation, Figure 12 is disease occurrence degree and capture sporangiums on the two and spore magnitude relation.
Embodiment
Below by specific embodiments and the drawings, the present invention will be further described.
Fig. 1 is that the spore of the present embodiment catches the structural representation of culture systems automatically.The system includes a casing 100,
It includes nor'wester 101 and three-dimensional bracket 102.Nor'wester 101 is used for rain cover.One is formed in the middle of three-dimensional bracket 102 to close
Accommodating cavity 103, inner cylinder 200, microcomputer control system 300 (control computer), negative-pressure air-flow blower fan 400 are placed in it.It is interior
The top of cylinder 200 sets an outer barrel 500, and it is a cylinder filter, and barrel is provided with multiple rows of pore, and aerial for filtering is big
Particle debris.Negative-pressure air-flow blower fan 400 is fixed on the bottom of accommodating cavity 103.The inwall of accommodating cavity 304 (can be with provided with heat-insulation layer
Using materials such as heat-preservation cottons), for reducing the temperature difference inside accommodating cavity and outside, it is ensured that the temperature of accommodating cavity internal stability.
Fig. 2A~Fig. 2 D are the structural representation of inner cylinder 200, and wherein Fig. 2A is that inner cylinder 200 removes after the barrel of top half
Dimensional structure diagram, 200a is string holes in the figure, includes the string holes and the string holes of control line of the transfusion line of culture apparatus,
200b is that (will be explained in detail hereinafter) at slide export.Fig. 2 B are to remove the dimensional structure diagram after whole barrels.Fig. 2 C
For Fig. 2 B top view, Fig. 2 D are the sectional view in C-C sections in Fig. 2 C.In order that the structure in picture shows clear, Fig. 2A, figure
Without display blast pipe 211 in 2B, and blast pipe 211 is illustrated in Fig. 2 C, Fig. 2 D.Screen pack is provided with blast pipe 211, is used
In the distinguished and admirable carry out secondary filter (being filtered through outer barrel 500 for the first time to realize) to entrance.
The structure composition in inner cylinder 200 is specifically described below：
A. glass box, slide and adhesive are carried
As shown in Figure 2 B, carrying glass box 201 is used to contain slide, carries the porch of glass box 201 provided with gear door 2011, its dozen
Multi-disc slide (such as 100) can be loaded after opening, the slide carried in glass box can be blocked after closing, it is to avoid it outwards falls
Go out.
Slide can be using concave-concave or single concavity slide, it is preferred to use concave-concave slide (has two grooves, is standard
Part).Adhesive is smeared in the recess of concave-concave slide, for bonding spore.Benefit using concave-concave slide is：A) in concave-concave
The recess of slide smears adhesive, and ensure that will not be mutually bonded between slide, and if using planar slide, then
Each slide after adhesive is smeared easily to bond together；B) later stage can add nutrient solution on concave-concave slide, it is ensured that spore gathers
Collection is at concave-concave, and spore will not spread, and is easy to gather spore pictorial information.
The composition of the adhesive used on slide is preferably, glycerine：Lactic acid：The volume ratio of tween=(3~5)：(1~
2)：(1~2), more preferably glycerine：Lactic acid：Tween=3：1：1.The wherein preferred Tween 80 of tween or polysorbas20.The bonding
In agent, glycerine is used to bond, and lactic acid is used for bacteria growing inhibiting, and tween is used for spore and disperses to avoid aggregation in heaps.By adhesive
Uniformly it is applied in the groove of concave-concave slide one end, is then placed in load glass box.
B. rotating disk and its drive mechanism
As shown in Fig. 2 C, Fig. 2 D, rotating disk 202 is driven by stepper motor 203.Rotating disk connecting shaft 204 connects following rotating disk
202 and travelling gear 205 above, and be fixed by bearing 206~208；The gear 2031 of stepper motor 203 and transmission
Gear 205 is engaged, and is rotated so as to driving rotating disk 202.210 be gear pressure plate, for fixed gear 205.
As shown in Fig. 2 B, Fig. 3, rotating disk 202 is provided with some grooves 2021 (being 5 grooves in figure), each groove
A piece of slide can be put into.Carry glass box 201, blast pipe 211 to be fixed on above rotating disk, the groove on rotating disk is turned under load glass box
Fang Shi, the slide 2012 carried in glass box 201 is fallen into groove；The groove equipped with slide is rotated further to entering with rotating disk afterwards
Below airduct, under fan action in the distinguished and admirable entrance blast pipe comprising spore, and spore is gathered by the adhesive on slide
Sub (spore is deposited on slide).Simultaneously as the rotation of rotating disk, makes next groove turn to below load glass box, so that
Another slide carried in glass box is fallen in the groove under gravity, and then rotating disk is rotated further and repeats above-mentioned mistake
Journey.That is, whenever the groove on rotating disk is turned under load glass box, just having a piece of slide in load glass box falls to groove
Upper (i.e. slide comes out from glass box is carried one by one).After acquisition, rotating disk is rotated further, and is driven slide and then is reached
Spore cultivation is carried out at the woven hose of culture apparatus, then rotating disk is rotated further, drive slide to reach under imaging device and carry out
After the completion of IMAQ, IMAQ, slide is released automatically from exit by ejecting device under driving stepper motor.Afterwards
Culture apparatus, imaging device and ejecting device will be described in detail in text.
Carrying in glass box, Counter weight metal block (such as piece of stainless steel) can be set on one piece of concave-concave slide of the top.This
Sample, when slide is left last two panels so as to be difficult to fall on groove, Counter weight metal block pushes down slide, and to fall into it recessed
In groove, i.e., the slide in load glass box is set to be fully used by the pressure effect of Counter weight metal block.
As shown in Figure 2 B, alignment sensor 209 can be set on gear 205 and (5 alignment sensors is shown in Fig. 2 B
209), the groove on locating turntable 202, makes groove be precisely located at load glass box, culture apparatus, the lower section of imaging device,
It can be used for calibration when error occurs in groove location etc..Alignment sensor 209 can be used arbitrarily with positioning function
Sensor, such as photoelectric sensor.
C. culture apparatus
Culture apparatus is used to add nutrient solution on the slide after collection spore, to cultivate spore.Culture dress
Putting can be inside inner cylinder 200, can also be outside inner cylinder 200.In the present embodiment, culture apparatus is outside inner cylinder 200
Portion, comprising infusion bag, woven hose, woven hose is entered in inner cylinder 200 by the string holes 200a shown in Fig. 2, and is fixed on rotating disk
Specific position on 202.
Fig. 4 A~Fig. 4 C are a kind of embodiment of culture apparatus 600, referred to as extrude type micro automatic liquid feeder, wherein
Fig. 4 A, Fig. 4 C are stereogram, and Fig. 4 B are top view.The device is provided with agravic infusion bag 601, and infusion bag 601 is connected with defeated
Liquid pipe 602 (illustrates two woven hoses) in figure, infusion bag 601, woven hose 602 are fixed in corresponding fixing groove 603,604；
Woven hose 602 uses one-way cock, and such infusion bag, which need not be hung, too high can ensure that nutrient solution will not flow back.The device is provided with
Stepper motor 605, control liquid feeding wheel 606 is rotated at regular time and quantity, is often rotated once, liquid feeding wheel 606 can extrude infusion bag 601 1
It is secondary, a drop nutrient solution is just had in woven hose 602 and flows out and drops on the slide in the groove 2021 on rotating disk 202, it is real
Existing automatic liquid-feeding culture.
D. imaging device
Imaging device 700 is illustrated in Fig. 5, wherein position shown in 701 includes microscope, video camera, microscope is used to put
Megaspore, is then taken pictures by video camera to it.Video camera is driven by stepper motor 702, and in microcomputer control system 300
Control is lower to be run.The lower section of stepper motor 702 is provided with slide unit 703, by the slide unit 703, and stepper motor 702 drives video camera by upper
Down successively take pictures.The slide unit can be realized using prior art, such as use ball slide unit.
In the present embodiment, imaging device 700 can also include transport module, for by wired or wireless way by shooting
Spore image transmitting is handled spore image, including automatically analyzed to Internet of Things server by Internet of Things server
Screening etc..
E. ejecting device
Ejecting device is used to that the release inner cylinder of the slide after spore image will to be acquired.Fig. 6 show ejecting device 800
A kind of implementation, it is comprising one the stepper motor 802 with screw mandrel 801, and one can be under driving stepper motor along screw mandrel
The movement arm 803 of motion.Stepper motor 802 is run under the control of microcomputer control system 300, will be acquired after spore image
Slide releases inner cylinder, and the groove after slide is released is rotated further to carrying under glass box, to fall into new slide.Fig. 7 is to push away
Go out the schematic diagram that device 800 releases slide 2012.200b is at slide export in Fig. 2.
F. the polycrystalline substance of inner cylinder 200
Fig. 8 is the polycrystalline substance schematic diagram of inner cylinder 200, includes front elevation (top view) and right view, lower view.Wherein
212 be rotary disk basement, i.e. the base of rotating disk 202；213 be condenser, for providing light source for the microscope in imaging device；214
For heating plate, the temperature for maintaining inner cylinder；215 be exhaust column, is connected with negative-pressure air-flow blower fan 400.
Fig. 9 is to carry out the flow chart that spore catches, cultivates and is imaged automatically using said apparatus, specifically includes following step
Suddenly：
1) spore automatic data collection
Spore automatic data collection step is using quantitative negative-pressure air-flow deposition principle.The own quantitative negative pressure function of blower fan, with reference to viscous
The spore that blower fan is extracted into is deposited on concave-concave slide by mixture.In collection, filtered first by outer barrel (cylinder filter)
Aerial debris, but the entrance of spore is not hindered, spore enters in the presence of the quantitative negative pressure exhausting of negative-pressure air-flow blower fan
To blast pipe, it is subsequently deposited upon on slide, is bonded on the adhesive coated on slide.There are screen pack, mistake in blast pipe
There is aperture on filter screen, it is possible to achieve secondary filter, the impurity (bulky grain debris) crossed in air filtering.The spore scope bag of collection
Include：Powdery Mildew, Pseudoperonospora cubensis, ash arrhizus bacteria, aspergillus germ, aecidium, Solanaceae early epidemic germ, saprophytic mould germ, late blight
The air-flows such as germ, brown patch germ, anthrax bacteria propagate germ and part soil-borne disease bacterium spore.
Above-mentioned gatherer process can realize the automatic data collection of spore, including automatic wind exhaust, change slide automatically.Automatically take out
Wind refers to control blower fan by microcomputer controller, realizes automatic wind exhaust.Automatic slide of changing refers to by microcomputer controller control
Rotating disk processed is rotated, and realizes the automatic replacing of slide, i.e.,：When groove on rotating disk is turned to below load glass box, carry in glass box
Slide is fallen into groove；The groove equipped with slide is rotated further to blast pipe with rotating disk afterwards, in the exhausting of blower fan
In the lower distinguished and admirable entrance blast pipe comprising spore of effect, and (spore is deposited on load by the adhesive collection spore on slide
On slide)；Meanwhile, next groove is turned to below load glass box, and another slide carried in glass box falls under gravity
Into the groove, then it is rotated further to being acquired below blast pipe, so repeatedly said process, is realized to spore with rotating disk
Continuous collecting.
2) nutrient solution is added automatically
Addition nutrient solution is with the purpose cultivated spore：To the environment of spore suitable growth, allow it to grow up, see
It can break out the disease of that type, and early-warning and predicting is carried out in real life.
Collection spore after slide be rotated further with rotating disk to culture apparatus, nutrient solution by woven hose at regular time and quantity
Ground is added on slide, realizes automatic liquid-feeding culture.The step can use previously described extruding type micro automatic liquid-feeding dress
Put to realize.Due to the outflow of nutrient solution can be controlled at regular time and quantity so that humidity during culture can be controlled, while inner cylinder
The heating plate that bottom is set can realize temperature adjustment, therefore can realize constant temperature, the culture environment of constant humidity.
When adding nutrient solution, 75-100 microlitres of addition every time.Nutrient solution mainly has following several：A, universal nutrient solution, contain
There is following component (mass percent)：Glucose 0%-0.3%, peptone 0.3%-0%；B, specific spore identification liquid, such as reflect
Determine to use concentrated hydrochloric acid during puccinia striiformis uredospore.
3) micro-imaging
Turn under microscope, camera system, carried out after microscope amplification by video camera by the spore after culture
Take pictures.The visual field can be changed automatically using Computer Control Technology, focused on automatically and be continuously shot (can be real using prior art
It is existing).
Specifically, one microlens can be installed in video camera front end, video camera is driven from top to bottom with stepper motor
Successively take pictures, every 0.001-0.01 millimeters of photo spacing of taking pictures, then by transport module, by wireless network transmissions to Internet of Things
Network server (i.e. following step 4).
4) spore image transmitting
After imaging, photo is transmitted to Internet of Things server by transport module.The Internet of Things server can be peace
Fill any one Internet of Things server of conditions permit, such as the agricultural ATCSP things of Hebi Jia Duo science, industry and trades limited company
Networked server.
Specifically, transport module can be using wire transmission or by the way of being wirelessly transferred by image transmitting to sub-services
Device, is then aggregated into central server by internet, to carry out spore Image Information Processing (i.e. following step 5).
5) spore image procossing
The step automatically analyzes effective picture that screening is collected using identification screening software.Analysis chart can such as be passed through
The light and shade contrast of the pixel of picture realizes screening, picks out more effective picture.If contrast is more than some value (such as
13000 etc.) image, is judged as to be clear, is otherwise judged as YES unclear, and then unclear image is removed, only stayed
Clear image down.Spore image after processing can by the Internet transmission to remote service end, such as remote computer service end,
Remote web page service end, remote handset service end etc..
Example：The correlation of forecast of potato late blight model and spore trap
Belgian (CARAH) model is thought：After humiture reaches suitable condition, the disease speed of development only with temperature phase
Close, related to humidity unobvious, after temperature is higher than 27 DEG C of critical point, disease is to stop circulating.The spore of the present invention is caught automatically
Seizure automatically, culture, micro- bat can be carried out to sporangium, the spore of the phytophthora infestans in air by catching culture assays system
According to, and the quantity of Spores is counted.Utilize the long-range monitoring and warning Internet of Things of Gansu Province's late blight of potato, analysis
The correlation of CARAH forecast of potato late blight model and spore trap, as a result shows：Journey occurs for CARAH model predictions disease
Degree and the spore of the present invention are caught between capture sporangiums on the two of culture assays system and spore amount automatically has notable positive
Guan Xing, coefficient R2For 0.9549.
1 material and method
The 1.1 long-range monitoring and warning Internet of Things of the material Gansu Province late blight of potato
1.2 methods are according to the plant disease prevention curve of the long-range monitoring and warning Internet of Things of Gansu Province's late blight of potato, and statistics is in advance
Disease occurrence degree, prediction is surveyed to infect time of origin, predict and infect the end time.Culture is caught according to the spore of the present invention automatically
The spore automated imaging picture statistics day of analysis system captures sporangium quantity and day capture spore quantity.Analyze CARAH models
Predict that disease occurrence degree captures the relation of spore quantity with capturing sporangium quantity and day day.
2 results and analysis
Prediction curve is done harm to by counting the Lintao station of Gansu in 2015 and Wudu station late blight of potato, data below is obtained：
The Lintao station late blight of potato evil prediction of table 1 and capture germ quantity surveying table
Note："-" indicates no prediction data
Wudu station late blight of potato evil prediction of table 2 and capture germ quantity surveying table
As can be seen from Table 1：On the day of disease infestation terminates and journey occurs for germ quantity and the disease of capture in second day
Degree there is certain correlation, if disease-free around here after the 3rd day infect generations, do not found in spore instrument sporangium with
Spore.
Based on the occurrence degree of disease and the germ quantity of capture, related data arrangement is carried out, 3, table is the results are shown in Table
4：
The forecast of potato late blight disease occurrence degree of table 3
The forecast of potato late blight disease occurrence degree of table 4 and germ quantitative relation
Based on the data of table 4, Linear correlative analysis is done to disease occurrence degree and the germ quantitative relation of capture, as a result
See Figure 10~Figure 12.It can be obtained from Figure 10~Figure 12：Disease occurrence degree and the correlation that the same day captures sporangium amount are poor, R2For
0.8508；Disease occurrence degree captured the good relationship of sporangium and spore amount, R with the same day2For 0.9265, disease generation journey
Spend the correlation for capturing sporangium and spore amount with the same day notable, y=1.2353x+3.1765, R2For 0.9549.
3 conclusions
By analyzing the correlation of CARAH forecast of potato late blight model and spore trap, as a result show：CARAH moulds
Type predicts that disease occurrence degree captured sporangium with the same day and spore amount has certain correlation；With two days capture sporangiums and
There is notable positive correlation between spore amount.The two can serve as the analysis of model prediction curve and germ quantitative relation, again
With capture sporangiums on the two and spore amount accuracy highest.
The above embodiments are merely illustrative of the technical solutions of the present invention rather than is limited, the ordinary skill of this area
Personnel can modify or equivalent substitution to technical scheme, without departing from the spirit and scope of the present invention, this
The protection domain of invention should be to be defined described in claims.
Claims (10)
1. a kind of spore catches system automatically, it is characterised in that the load of slide is housed by motor-driven rotating disk, one including one
Glass box, a blast pipe, a blower fan, one by motor-driven ejecting device, and a control computer；Rotating disk, ejecting device and
Blower fan is controlled to run by control computer；Glass box, blast pipe and ejecting device is carried to be fixed on above rotating disk；Rotating disk is provided with least
When groove on one groove, rotating disk is turned to below load glass box, the slide carried in glass box is fallen into groove；Afterwards equipped with load
The groove of slide is rotated further to blast pipe with rotating disk, under fan action in the distinguished and admirable entrance blast pipe comprising spore,
And spore is gathered by the adhesive on slide；Slide is released into groove by ejecting device afterwards.
2. system as claimed in claim 2, it is characterised in that：The groove is at least two, and the load glass box is built with more
Piece slide；First slide falls into the first groove and turned to below blast pipe to gather during spore, the second groove position
In carrying below glass box and falling into second slide, second slide turns to below blast pipe to gather spore afterwards；Adopt
Collect the slide launched device after spore to release after groove, the groove is rotated further to load glass box and falls into the 3rd load
Slide；So realize lasting collection.
3. the system as claimed in claim 1, it is characterised in that：Also include culture apparatus, its comprising infusion bag, woven hose and by
The motor-driven pressurizing unit for being used to extrude infusion bag, the infusion bag is built with nutrient solution, and the pressurizing unit is by described
Control computer control operation；Slide after collection spore is rotated further to the culture apparatus with rotating disk, in the control
Under the control of computer and the driving of motor, by pressurizing unit extrude infusion bag, make nutrient solution by woven hose at regular time and quantity
Ground is added on slide, realizes automatic liquid-feeding culture；Slide is released into groove by ejecting device afterwards.
4. system as claimed in claim 3, it is characterised in that：The groove is at least three, and the load glass box is built with more
Piece slide；First slide falls into the first groove and turned to below blast pipe to gather during spore, the second groove position
In carrying below glass box and fall into second slide；First slide after collection spore is rotated further defeated to culture apparatus
When below liquid pipe to be cultivated, second slide is turned to below blast pipe to gather spore, while the 3rd groove is located at
Carry below glass box and fall into the 3rd slide；So realize lasting collection and culture.
5. system as claimed in claim 3, it is characterised in that：The nutrient solution is 0%~0.3% comprising mass percent
Glucose, and the peptone that mass percent is 0%~0.3%.
6. system as claimed in claim 3, it is characterised in that：Also include imaging device, it includes microscope and video camera, its
Middle video camera is driven by motor, and controls operation by the control computer；Spore after culture turns to imaging dress
Put down, drive video camera to be taken pictures by motor after amplifying through microscope；Slide is released into groove by ejecting device afterwards.
7. system as claimed in claim 6, it is characterised in that：The groove is at least four, and the load glass box is built with more
Piece slide；First slide falls into the first groove and turned to below blast pipe to gather during spore, the second groove position
In carrying below glass box and fall into second slide；First slide after collection spore is rotated further defeated to culture apparatus
When below liquid pipe to be cultivated, second slide is turned to below blast pipe to gather spore, while the 3rd groove is located at
Carry below glass box and fall into the 3rd slide；First slide after being cultivated is rotated further to imaging device
When, second slide is located at below the woven hose of culture apparatus to be cultivated, while the 3rd groove is located at below blast pipe
To gather spore, while the 4th groove is in load glass box lower section and falls into the 4th slide；So realize lasting collection, culture
And imaging.
8. system as claimed in claim 6, it is characterised in that：The imaging device includes transport module, for by wired
Or wireless mode is by the spore image transmitting of shooting to Internet of Things server；The Internet of Things server to spore image at
Reason, including carry out automatically analyzing screening.
9. the system as described in any claim in claim 1 to 8, it is characterised in that：The blower fan is quantitative negative pressure gas
Flow fan；The motor is stepper motor；The slide is single concavity slide or concave-concave slide, smears viscous in its recess
Mixture；Described adhesive includes glycerine, lactic acid and tween；Filter is provided with the import of the blast pipe, for filtering sky
In bulky grain debris；Screen pack is provided with the blast pipe, for the distinguished and admirable carry out secondary filter to entrance.
10. the system as described in any claim in claim 1 to 8, it is characterised in that：Also include an externally-located case
Body, the casing includes a nor'wester and a three-dimensional bracket.
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| CN109212737A (en) * | 2018-10-25 | 2019-01-15 | 西北农林科技大学 | A kind of fungal spore microscopic image acquisition device that collection point is controllable and method |
| CN109491069A (en) * | 2018-10-25 | 2019-03-19 | 西北农林科技大学 | A kind of Air Fungi spore micro-image remote collecting device and method |
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Application publication date: 20170811 |