CN106978446A - A kind of method that utilization stalk prepares hydrogen - Google Patents
A kind of method that utilization stalk prepares hydrogen Download PDFInfo
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- CN106978446A CN106978446A CN201710214780.3A CN201710214780A CN106978446A CN 106978446 A CN106978446 A CN 106978446A CN 201710214780 A CN201710214780 A CN 201710214780A CN 106978446 A CN106978446 A CN 106978446A
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- stalk
- hydrogen
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- rumen microorganism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
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- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Processing Of Solid Wastes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of method that utilization stalk prepares hydrogen, on the basis of traditional handicraft, rumen microorganism has not only been used in the domestication rumen microorganism stage, it is also added into scotobacteria and cellulase simultaneously, accelerate the degradation speed of stalk, so as to accelerate the adding speed and processing speed of stalk in whole course of reaction, accelerate the efficiency of hydrogen making, especially degradation efficiency reaches 77 82%, also there is small size raising.
Description
Technical field
The present invention relates to bio-hydrogen production technology field, more particularly to a kind of method that utilization stalk prepares hydrogen.
Background technology
China produces substantial amounts of phyteral and its discarded object every year, wherein agricultural crop straw annual production just more than 600,000,000 tons,
300,000,000 tons of standard coals are about converted into, this is a huge energy reserves.Annual rural area is in harvest season all a large amount of crop straw burnings, both
Waste valuable and limited resource, and severe contamination environment.Strengthen waste resource to recycle and environmental protection, be conducive to
Promote the ecological balance, the need for being also China's sustainable economic development.Therefore, to the application technology as the second resource of botanical waste
Exploitation tool be of great significance.
Hydrogen is as a kind of important industrial chemicals, in many fields such as petrochemical industry, electronics industry and middle small power plants all
It is widely used.Hydrogen is also a kind of important clean energy resource, is a kind of important fuel in aerospace industry.Hydrogen
It is that can realize the clean energy resource of zero emission, therefore will in following electric automobile, public transport and hydrogen power generation
With important purposes.The fuel value of hydrogen is high, is 3 times of gasoline, 3.9 times of alcohol；In cleanliness without any pollution, combustion process only
Water is produced, GHG carbon dioxide and other harmful materials are not produced.
In the prior art, the preparation of hydrogen includes physico-chemical process and the major class of biological technique method two.Physical chemistry side
Method prepares hydrogen technology maturation, is existing main hydrogen preparation method, but its high energy consumption, costly.With physical chemistry side
Method is compared, biological method hydrogen making have efficiently, energy-conservation, it is economical many advantages, such as and extremely countries in the world and numerous international group
The great attention knitted.
Chinese invention patent CN200410065683.5 discloses a kind of efficient utilization botanical waste and prepares hydrogen
Method, this method is degraded using rumen microorganism to botanical waste, although cost is low, and effect is good, but takes
It is long, in order to shorten the reaction time, it is necessary to which it is made further research.
The content of the invention
The technical problem existed based on background technology, the present invention proposes a kind of method that utilization stalk prepares hydrogen.
A kind of method that utilization stalk prepares hydrogen, is comprised the following steps that：
A, broken phyteral：By crops stalk crushing, stalk particle is obtained；
B, domestication rumen microorganism：Tamed, connect from being taken out in ruminant tumor gastric during rumen microorganism is inoculated into fermentation tank
Plant and stalk particle and mineral matter buffer solution are filled in 6-10% of the amount for fermentation tank reaction volume, the fermentation tank, and add dark thin
Bacterium and cellulase；
C, startup anaerobic digestion reaction vessel：Rumen microorganism after domestication is inoculated into anaerobic digestion reaction vessel, 5-10 times is passed through
In the remaining oxygen of the inert gas removal of reactor volume, 50-60 DEG C of maintenance reaction area temperature, pH is 5.0-5.5, stirring speed
Rate is 180-200 revs/min, and the concentration after stalk dilutes through fragrant buffer solution of wearing is 100-120g/L；When microorganism in reaction zone
Concentration reaches more than 2.0g/L and keeps stable operation 2 weeks, then anaerobic digestion reaction vessel start completion；
D, the hydrolysis acidification of phyteral simultaneously collect hydrogen gas：The anaerobic digestion reaction vessel of stable state is reached, according to stalk
The stalk concentration of the corresponding adjustment buffer solution dilution of difference of adding rate, the adding rate mobility scale of stalk is 80-120g/L/
D, hydraulic detention time is 6-10 hours, and the stalk residence time is 8-12 hours, and stir speed (S.S.) maintains 150-200 revs/min,
Reaction zone pH scopes are 5.0-5.5；Collect hydrogen content in the hydrogen gas produced, the gas and reach 10-30%, by letter
Simplification processing obtains pure hydrogen.
It is preferred that, in described step A, the particle diameter of described stalk particle is 1-3mm.
It is preferred that, the condition of described rumen microorganism domestication is：Stalk adding rate is 20-40g/L/d, hydraulic retention
Time 8-12 hour, pH is 5.0-5.5, and temperature is 45-50 DEG C, 120-150 revs/min of stir speed (S.S.), and stalk is in reactor
Residence time 8-12 hour.
It is preferred that, in described step B, the inoculum concentration of scotobacteria is 3-5%, and the addition of cellulase is stalk quality
0.002-0.005%.
This programme is beneficial in that compared to traditional scheme：The present invention is on the basis of traditional handicraft, in domestication knurl
The stomach microorganism stage has not only used rumen microorganism, while being also added into scotobacteria and cellulase, accelerates the degraded of stalk
Speed, so as to accelerate the adding speed and processing speed of stalk in whole course of reaction, accelerates the efficiency of hydrogen making, especially
It is that degradation efficiency reaches 77-82%, also there is small size raising.
Embodiment
Embodiment 1：
A kind of method that utilization stalk prepares hydrogen, is comprised the following steps that：
A, broken phyteral：By crops stalk crushing, stalk particle is obtained；In described step A, described stalk particle
Particle diameter is 1-3mm；
B, domestication rumen microorganism：Tamed, connect from being taken out in ruminant tumor gastric during rumen microorganism is inoculated into fermentation tank
Plant and stalk particle and mineral matter buffer solution are filled in 6-10% of the amount for fermentation tank reaction volume, the fermentation tank, and add dark thin
Bacterium and cellulase；The inoculum concentration of scotobacteria is 3-5%, and the addition of cellulase is the 0.002-0.005% of stalk quality；
The condition of described rumen microorganism domestication is：Stalk adding rate is 20-40g/L/d, and hydraulic detention time 8-12 is small
When, pH is 5.0-5.5, and temperature is 45-50 DEG C, 120-150 revs/min of stir speed (S.S.), residence time of the stalk in reactor
8-12 hours；
C, startup anaerobic digestion reaction vessel：Rumen microorganism after domestication is inoculated into anaerobic digestion reaction vessel, 5-10 times is passed through
In the remaining oxygen of the inert gas removal of reactor volume, 50-60 DEG C of maintenance reaction area temperature, pH is 5.0-5.5, stirring speed
Rate is 180-200 revs/min, and the concentration after stalk dilutes through fragrant buffer solution of wearing is 100-120g/L；When microorganism in reaction zone
Concentration reaches more than 2.0g/L and keeps stable operation 2 weeks, then anaerobic digestion reaction vessel start completion；
D, the hydrolysis acidification of phyteral simultaneously collect hydrogen gas：The anaerobic digestion reaction vessel of stable state is reached, according to stalk
The stalk concentration of the corresponding adjustment buffer solution dilution of difference of adding rate, the adding rate mobility scale of stalk is 80-120g/L/
D, hydraulic detention time is 6-10 hours, and the stalk residence time is 8-12 hours, and stir speed (S.S.) maintains 150-200 revs/min,
Reaction zone pH scopes are 5.0-5.5；Collect hydrogen content in the hydrogen gas produced, the gas and reach 10-30%, by letter
Simplification processing obtains pure hydrogen.
Embodiment 2
A kind of method that utilization stalk prepares hydrogen, is comprised the following steps that：
A, broken phyteral：By crops stalk crushing, stalk particle is obtained；In described step A, described stalk particle
Particle diameter is 1-3mm；
B, domestication rumen microorganism：Tamed, connect from being taken out in ruminant tumor gastric during rumen microorganism is inoculated into fermentation tank
Plant and stalk particle and mineral matter buffer solution are filled in 6-10% of the amount for fermentation tank reaction volume, the fermentation tank, and add dark thin
Bacterium and cellulase；The inoculum concentration of scotobacteria is 3-5%, and the addition of cellulase is the 0.002-0.005% of stalk quality；
The condition of described rumen microorganism domestication is：Stalk adding rate is 20-40g/L/d, and hydraulic detention time 8-12 is small
When, pH is 5.0-5.5, and temperature is 45-50 DEG C, 120-150 revs/min of stir speed (S.S.), residence time of the stalk in reactor
8-12 hours；
C, startup anaerobic digestion reaction vessel：Rumen microorganism after domestication is inoculated into anaerobic digestion reaction vessel, 5-10 times is passed through
In the remaining oxygen of the inert gas removal of reactor volume, 50-60 DEG C of maintenance reaction area temperature, pH is 5.0-5.5, stirring speed
Rate is 180-200 revs/min, and the concentration after stalk dilutes through fragrant buffer solution of wearing is 100-120g/L；When microorganism in reaction zone
Concentration reaches more than 2.0g/L and keeps stable operation 2 weeks, then anaerobic digestion reaction vessel start completion；
D, the hydrolysis acidification of phyteral simultaneously collect hydrogen gas：The anaerobic digestion reaction vessel of stable state is reached, according to stalk
The stalk concentration of the corresponding adjustment buffer solution dilution of difference of adding rate, the adding rate mobility scale of stalk is 80-120g/L/
D, hydraulic detention time is 6-10 hours, and the stalk residence time is 8-12 hours, and stir speed (S.S.) maintains 150-200 revs/min,
Reaction zone pH scopes are 5.0-5.5；Collect hydrogen content in the hydrogen gas produced, the gas and reach 10-30%, by letter
Simplification processing obtains pure hydrogen.
Embodiment 3
A kind of method that utilization stalk prepares hydrogen, is comprised the following steps that：
A, broken phyteral：By crops stalk crushing, stalk particle is obtained；In described step A, described stalk particle
Particle diameter is 1-3mm；
B, domestication rumen microorganism：Tamed, connect from being taken out in ruminant tumor gastric during rumen microorganism is inoculated into fermentation tank
Plant and stalk particle and mineral matter buffer solution are filled in 6-10% of the amount for fermentation tank reaction volume, the fermentation tank, and add dark thin
Bacterium and cellulase；The inoculum concentration of scotobacteria is 3-5%, and the addition of cellulase is the 0.002-0.005% of stalk quality；
The condition of described rumen microorganism domestication is：Stalk adding rate is 20-40g/L/d, and hydraulic detention time 8-12 is small
When, pH is 5.0-5.5, and temperature is 45-50 DEG C, 120-150 revs/min of stir speed (S.S.), residence time of the stalk in reactor
8-12 hours；
C, startup anaerobic digestion reaction vessel：Rumen microorganism after domestication is inoculated into anaerobic digestion reaction vessel, 5-10 times is passed through
In the remaining oxygen of the inert gas removal of reactor volume, 50-60 DEG C of maintenance reaction area temperature, pH is 5.0-5.5, stirring speed
Rate is 180-200 revs/min, and the concentration after stalk dilutes through fragrant buffer solution of wearing is 100-120g/L；When microorganism in reaction zone
Concentration reaches more than 2.0g/L and keeps stable operation 2 weeks, then anaerobic digestion reaction vessel start completion；
D, the hydrolysis acidification of phyteral simultaneously collect hydrogen gas：The anaerobic digestion reaction vessel of stable state is reached, according to stalk
The stalk concentration of the corresponding adjustment buffer solution dilution of difference of adding rate, the adding rate mobility scale of stalk is 80-120g/L/
D, hydraulic detention time is 6-10 hours, and the stalk residence time is 8-12 hours, and stir speed (S.S.) maintains 150-200 revs/min,
Reaction zone pH scopes are 5.0-5.5；Collect hydrogen content in the hydrogen gas produced, the gas and reach 10-30%, by letter
Simplification processing obtains pure hydrogen.
Reaction zone microorganism concn reaches 8-15g/L when reaching poised state.Hydrogen generation efficiency reaches 120-140ml/g
Degrading straw, the sour efficiency of production reaches 0.5-0.6g organic acids/g stalks, and degradation efficiency reaches 77-82%.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should all be included within the scope of the present invention.
Claims (4)
1. a kind of method that utilization stalk prepares hydrogen, it is characterised in that comprise the following steps that：
A, broken phyteral：By crops stalk crushing, stalk particle is obtained；
B, domestication rumen microorganism：Tamed, connect from being taken out in ruminant tumor gastric during rumen microorganism is inoculated into fermentation tank
Plant and stalk particle and mineral matter buffer solution are filled in 6-10% of the amount for fermentation tank reaction volume, the fermentation tank, and add dark thin
Bacterium and cellulase；
C, startup anaerobic digestion reaction vessel：Rumen microorganism after domestication is inoculated into anaerobic digestion reaction vessel, 5-10 times is passed through
In the remaining oxygen of the inert gas removal of reactor volume, 50-60 DEG C of maintenance reaction area temperature, pH is 5.0-5.5, stirring speed
Rate is 180-200 revs/min, and the concentration after stalk dilutes through fragrant buffer solution of wearing is 100-120g/L；When microorganism in reaction zone
Concentration reaches more than 2.0g/L and keeps stable operation 2 weeks, then anaerobic digestion reaction vessel start completion；
D, the hydrolysis acidification of phyteral simultaneously collect hydrogen gas：The anaerobic digestion reaction vessel of stable state is reached, according to stalk
The stalk concentration of the corresponding adjustment buffer solution dilution of difference of adding rate, the adding rate mobility scale of stalk is 80-120g/L/
D, hydraulic detention time is 6-10 hours, and the stalk residence time is 8-12 hours, and stir speed (S.S.) maintains 150-200 revs/min,
Reaction zone pH scopes are 5.0-5.5；Collect hydrogen content in the hydrogen gas produced, the gas and reach 10-30%, by letter
Simplification processing obtains pure hydrogen.
2. the method for preparing hydrogen using stalk as claimed in claim 1, it is characterised in that described in described step A
The particle diameter of stalk particle is 1-3mm.
3. the method for preparing hydrogen using stalk as claimed in claim 1, it is characterised in that described rumen microorganism domestication
Condition be：Stalk adding rate is 20-40g/L/d, and hydraulic detention time 8-12 hours, pH is 5.0-5.5, and temperature is 45-
50 DEG C, 120-150 revs/min of stir speed (S.S.), residence time 8-12 hour of the stalk in reactor.
4. the method for preparing hydrogen using stalk as claimed in claim 1, it is characterised in that in described step B, scotobacteria
Inoculum concentration be 3-5%, the addition of cellulase is the 0.002-0.005% of stalk quality.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710214780.3A CN106978446A (en) | 2017-04-01 | 2017-04-01 | A kind of method that utilization stalk prepares hydrogen |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710214780.3A CN106978446A (en) | 2017-04-01 | 2017-04-01 | A kind of method that utilization stalk prepares hydrogen |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108395989A (en) * | 2018-05-24 | 2018-08-14 | 郑州工程技术学院 | A kind of straw saccharification fermentation reactor |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1772403A (en) * | 2004-11-09 | 2006-05-17 | 中国科学技术大学 | Method of utilizing rumen microbe in degrading waste plant matter effectively |
| WO2008076423A1 (en) * | 2006-12-18 | 2008-06-26 | University Of Maryland | Process for rapid anaerobic digestion of biomass using microbes and the production of biofuels therefrom |
-
2017
- 2017-04-01 CN CN201710214780.3A patent/CN106978446A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1772403A (en) * | 2004-11-09 | 2006-05-17 | 中国科学技术大学 | Method of utilizing rumen microbe in degrading waste plant matter effectively |
| WO2008076423A1 (en) * | 2006-12-18 | 2008-06-26 | University Of Maryland | Process for rapid anaerobic digestion of biomass using microbes and the production of biofuels therefrom |
Non-Patent Citations (4)
| Title |
|---|
| 俞联平: "《草食家畜营养及饲料加工调制技术》", 31 July 2014 * |
| 温辉梁: "《饲料添加剂生产技术与配方》", 31 December 2009 * |
| 章力建: "《农业立体污染综合防治理论与实践 江河流域平原卷》", 31 December 2013 * |
| 马庆生: "《生物学大辞典》", 31 January 1999 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108395989A (en) * | 2018-05-24 | 2018-08-14 | 郑州工程技术学院 | A kind of straw saccharification fermentation reactor |
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Application publication date: 20170725 |