CN106955750B - Micro-fluidic chip based on the flowing of active control liquid - Google Patents
Micro-fluidic chip based on the flowing of active control liquid Download PDFInfo
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- CN106955750B CN106955750B CN201710213382.XA CN201710213382A CN106955750B CN 106955750 B CN106955750 B CN 106955750B CN 201710213382 A CN201710213382 A CN 201710213382A CN 106955750 B CN106955750 B CN 106955750B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K99/0001—Microvalves
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K2099/0082—Microvalves adapted for a particular use
- F16K2099/0084—Chemistry or biology, e.g. "lab-on-a-chip" technology
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Abstract
The invention discloses a kind of micro-fluidic chips based on the flowing of active control liquid, including sample introduction chamber, reaction chamber, waste chamber and micro-fluidic runner, sample introduction chamber to be connected to gas circuit；The micro-valve in normally open is installed on the micro-fluidic runner, which has and prevent the micro-valve Anti-backflow structure of fluid reflux and whether flow through the testing agency of micro-valve installation site for sensing fluid；Micro-valve in normally open can be closed automatically when the numerical tabular free flow body that testing agency is fed back flows through micro-valve installation site, be in current floe condition；Micro-valve in current floe condition can be opened periodically, and circulation status is in；Micro-valve in circulation status, in the case where the air-flow that gas circuit provides pushes, fluid can circulate in micro-valve.It it follows that micro-fluidic chip of the present invention can arbitrarily control the flow velocity of fluid in entire micro-fluidic runner, and can be fixed in a certain region, carry out incubation reaction.
Description
Technical field
The present invention relates to a kind of micro-fluidic chips based on the flowing of active control fluid.
Background technology
Immune lateral chromatography diagnostic techniques is suitble to as a kind of stabilization and practical technology in various real-time test
(POCT) or onsite application.
In immune series of strata reaction system, causes CV big due to system, be unable to reach accurate quantification.And it is based on miniflow
The immune diagnostic method of control technology can effectively avoid the above problem.
The micro-fluidic passive type and two kinds active of being divided into.Passive type is micro-fluidic still to need capillary power to reach liquid
Forward lateral chromatography.But due to the viscosity of different samples especially whole blood sample difference, cause flow rate of liquid without legally constituted authority
One.
It is active micro-fluidic it is possible to prevente effectively from the above problem, forward thrust can be given, liquid be made uniformly to flow forward
It is dynamic, it avoids because of test value difference caused by different in flow rate.
Active micro-fluidic power have centrifugation power drive, electrowetting driving, pressure-driven (electrolysis pump, compressed gas pump,
Chemical breakdown pump, the driving of direct draught head)
But if to achieve the purpose that arbitrarily to control liquid velocity, not only to have motive force, also to there is valve control, also
There is anti-return so as not to liquid, because pressure removes, reflux is gone back.
Existing the relevant technologies, the making of chip and the application of chip, please refer to following patent：
1) a kind of micro-fluidic chips of CN203899622U
2) the micro-fluidic bedside's quick diagnosis reagent kits of CN106353491A
3) CN205941345U is used for the micro-fluidic chip of biological detection
Wherein, patent 1) in, it is not directed to mixed function, and different liquids and liquid and solid in chip (such as it is pre-buried
Freeze-dried reagent etc.) mixing be micro-fluidic chip a key function.
Patent 2) and 3) in, the sample liquid being added in chip is not quantified, and to realize quantitative detection, it is necessary to
It realizes to the quantitative of the sample of addition and is pre-placed quantifying in the reagent in chip.
Patent 1), 2) and 3) in be all not directed to the monitoring of accurate location when liquid flows in the chips, it is in other words, above-mentioned
The chip of 3 patents before last testing result to fluid, do not supervise in the chip by the filling behavior of channel or cavity
It surveys.
Invention content
In view of the deficiencies of the prior art, the present invention provides it is a kind of based on active control fluid flowing micro-fluidic chip,
The thrust that fluid flows in micro-fluidic runner is provided by configuring special micro-valve in micro-fluidic runner, and using gas circuit so that
Fluid in micro-fluidic runner of the present invention can active control, can arbitrarily control fluid in entire micro-fluidic runner
Flow velocity, and can be fixed in a certain region, carry out incubation reaction.Reach real micro-fluidic purpose.In addition, the present invention passes through
Quantitative chamber reaches quantitative sample-adding truly, and redundant sample is discharged into waste chamber.Further more, fully mixed by evenly mixing device
Even sample provides a strong guarantee for properties of product.Last technical purpose be there is sealing ring made of conductive rubber,
It is played together with device capacitances detection probe, the detection of liquid flowing state.
For the technical purpose for realizing above-mentioned, the present invention will take the following technical solution：
It is a kind of based on active control liquid flowing micro-fluidic chip, including sample introduction chamber, reaction chamber, waste chamber with
And micro-fluidic runner, sample introduction chamber are connected to gas circuit；The micro-valve in normally open is installed on the micro-fluidic runner, this is micro-
Valve has and prevents the micro-valve Anti-backflow structure of fluid reflux and whether flow through the detection of micro-valve installation site for sensing fluid
Mechanism；Micro-valve in normally open can when the numerical tabular free flow body that testing agency is fed back flows through micro-valve installation site
It is automatic to be closed, it is in current floe condition；Micro-valve in current floe condition can be opened periodically, and circulation status is in；In circulation shape
The micro-valve of state, in the case where the air-flow that gas circuit provides pushes, fluid can circulate in micro-valve.
As a further improvement on the present invention, the micro-valve includes the first micro-valve, the second micro-valve；The sample introduction chamber with it is anti-
It answers and is provided with quantitative chamber between chamber；Micro-fluidic runner at sample introduction chamber liquid outlet is divided into two-way, respectively micro-fluidic master
Runner and micro-fluidic bypass runner；Sample introduction chamber by micro-fluidic sprue successively with quantitative chamber, reaction chamber, waste chamber
Connection, while sample introduction chamber is connected to by micro-fluidic bypass runner with waste chamber；It is micro- between sample introduction chamber and waste chamber
The first micro-valve is installed on flow control bypass runner；It is micro- that quantitative chamber installs second on the micro-fluidic sprue of liquid outlet
Valve；The first Anti-backflow structure is installed on micro-fluidic sprue between sample introduction chamber and quantitative chamber；In the first micro-valve, second
Micro-valve, the first Anti-backflow structure cooperation under, quantitative chamber can be according to own volume size to reaction chamber weight feed sample
Product.
As a further improvement on the present invention, described quantify has evenly mixing device, the mixing between chamber and reaction chamber
Device includes mixing chamber and mixing runner；The Z-shape shape of mixing runner；The micro-fluidic sprue of mixing chamber both sides is at least
Part is mixing runner, and mixing chamber can be with mixing runner Natural communication.
Another technical object of the present invention is to provide a kind of micro-fluidic chips based on the flowing of active control liquid, including into
Sample chamber, sample mark-quantify chamber, fluid path runner, mixing chamber, reaction chamber, waste chamber and micro-fluidic runner；Its
In：The sample introduction chamber is used to prepare simultaneously filtered sample；The sample marks-chamber is quantified, it is quantitative according to own volume size
It receives sample introduction chamber and conveys the sample come, and exported after sample and marker reaction；The mixing chamber is used for sample mark
The sample blending of the quantitative chamber output of note-；The reaction chamber is embedded with the coating body to react with marker, has reacted
At rear output to waste chamber；Fluid path runner, each chamber for cleaning micro-fluidic runner and on micro-fluidic runner,
One end and external fluid path orifice, the other end are then connected to micro-fluidic runner；Micro-fluidic runner include micro-fluidic sprue and
Micro-fluidic bypass runner；Sample introduction chamber is marked with sample-is quantified successively chamber by micro-fluidic sprue, mixing chamber, reacts
Chamber, waste chamber connection, while sample introduction chamber is connected to by micro-fluidic bypass runner with waste chamber；Sample introduction chamber and waste liquid
The first micro-valve is installed on micro-fluidic bypass runner between chamber；Sample introduction chamber and sample mark-quantify micro- between chamber
First anti-return runner is installed on flow control sprue；Sample marks-quantifies the micro-fluidic mainstream between chamber and mixing chamber
Second micro-valve is installed on road；On micro-fluidic sprue between mixing chamber and reaction chamber in close proximity to reaction chamber into
Liquid mouth is disposed with the second Anti-backflow structure at position, and is equipped on the micro-fluidic sprue between reaction chamber and waste chamber
Third micro-valve；The first anti-return runner, the second Anti-backflow structure include anti-return runner；The anti-return runner
The extending direction of extending direction and the micro-fluidic runner of Anti-backflow structure installed position is perpendicular；Anti-backflow structure installed position
Micro-fluidic runner be separated into two sections by micro-fluidic runner spacer block, respectively micro-fluidic feed liquor runner, it is micro-fluidic go out liquid stream road；
The upper end closed of anti-return runner, lower end are then connected across above micro-fluidic runner spacer block, and respectively with micro-fluidic feed liquor runner, micro-
Flow control goes out the connection of liquid stream road；When connecting gas circuit, under the air pressure driving that gas circuit provides, the fluid stream in micro-fluidic feed liquor runner
After anti-return runner, be back to it is micro-fluidic go out liquid stream road in；First micro-valve, the second micro-valve, third micro-valve are to prevent back
Flow micro-valve, including press mechanism, testing agency, sealing element and micro-valve Anti-backflow structure；Wherein：The micro-valve anti-return knot
Structure, the micro-fluidic runner split settings with anti-return micro-valve installed position include the micro-valve anti-return runner of perforation；It is described anti-
The micro-fluidic runner of reflux micro-valve installed position is divided into micro-valve liquid input section by micro-fluidic runner tabula and micro-valve goes out liquid section；It is micro-
Valve liquid input section has liquid outlet, and micro-valve, which goes out liquid section, has inlet；It is horizontal that the lower end of micro-valve anti-return runner is placed in micro-fluidic runner
Every top, and can to go out the inlet of liquid section with the liquid outlet of micro-valve liquid input section, micro-valve respectively corresponding for micro-valve anti-return runner
Connection；The upper end of micro-valve anti-return runner has the caulking groove for installing sealing element；The sealing element is conductive seal circle, packet
Include outer layer sealing ring, internal close membrane and capacitance probe contact；Internal close membrane is in the inner ring of outer layer sealing ring, and capacitance
Probe contacts are then connect with outer layer sealing ring；Sealing element is flush-mounted in caulking groove, and internal close membrane covers micro-valve and prevents back just
The upper end of runner is flowed, while internal close membrane has the space of free deformation；The press mechanism, including push-down head, under this
The top of the internally positioned close membrane of pressure head；The testing agency, including detection probe, the detection probe connect with push-down head linkage
It connects, and positioned at the top of probe contacts, while the push-down head that is smaller than between detection probe and probe contacts is closed with internal
Spacing between film；When the push-down head of press mechanism with detection probe towards conductive seal circle downlink when, detection probe with
When probe contacts touch, press mechanism stops downlink, at this point, there are spacing between push-down head and internal close membrane；When detection is visited
The data of head sensing change, and show there are when the micro-fluidic runner that fluid flows through valving installed position, under triggering
Press mechanism acts, and push-down head is pushed to continue traveling downwardly, until push-down head pushes down internal close membrane, to seal micro-valve liquid input section simultaneously
Liquid outlet, micro-valve go out the inlet of liquid section, and valving is in current floe condition at this time；When the push-down head of press mechanism is sealed with internal
It closes between film there are when gap, valving is in the open state, deformation characteristics possessed by internal close membrane, it is ensured that fluid energy
Enough to be flowed out from the liquid outlet of micro-valve liquid input section, the inlet that liquid section is gone out from micro-valve flows into.
According to above-mentioned technical solution, compared with the existing technology, the present invention has following advantage：
1, the present invention has proportioning device in the chip, is made of the first micro-valve, the second micro-valve and the first Anti-backflow structure,
Really quantitative sample-adding is played, the difference of sample-adding amount caused by avoiding pipettor not calibrated provides reliable together for quantitative test
Insurance.
2, the present invention mixes well sample by evenly mixing device, is provided a strong guarantee for properties of product.
3, the present invention creatively devises valving, back-flow preventer, and combines gas circuit as liquid is forward and push away
Power can arbitrarily control liquid velocity in runner, and can be fixed in a certain region, carry out incubation reaction.Reach real
Micro-fluidic purpose.
4, the present invention is having sealing ring made of conductive rubber and device capacitances detection probe to play together, liquid flow
The detection of dynamic state.
Description of the drawings
Fig. 1 is the structural schematic diagram of micro-fluidic chip of the present invention；
In figure：Upper layer chip 1；Middle level chip 2；Lower layer chip 3；Push-down head 4；Capacitance probe 5；External fluid path interface 6；Outside
Connect air-path interface 7；Blotting paper 8；It is coated with body 9；Hemofiltration film 10；Conductive seal circle 11；
Fig. 2 is the structural schematic diagram of upper layer chip of the present invention；
In figure：First micro-valve push-down head mounting hole 1-1；Second micro-valve push-down head passage hole hole 1-2；Third micro-valve push-down head
Current hole 1-3；The upper cover 1-4 of reactive tank；Fluid path interface 1-5；Air-path interface 1-6；The pillar 1-7 of upper layer chip；
Fig. 3 is the structural schematic diagram of middle level chip of the present invention；
In figure：Middle level chip 2；First caulking groove 2-1；Second caulking groove 2-2；Third caulking groove 2-3；First Anti-backflow structure is in
Position 2-4 on layer chip；The second Anti-backflow structure position 2-5 on chip in middle level；Fluid path interface 2-6；Reactive tank straight slot
2‐7；Air-path interface 2-8；Waste liquid pool upper cover 2-9；The pillar 2-10 of middle level chip；Loading slot 2-11；
Fig. 4 a are the structural schematic diagrams of lower layer chip of the present invention；
In figure：Lower layer chip 3；Sample inlet pool 3-1；Sample treatment-quantifies pond 3-2；The micro-fluidic runner 3-3 of Z-type；Fluid path runner
3‐4；First mixing slot 3-5；Second mixing slot 3-6；Reactive tank 3-7；Waste liquid tank 3-8；The runner 3-9 of first micro-valve；Second is micro-
The runner 3-10 of valve；The runner 3-11 of third micro-valve；The micro-fluidic runner 3-12 of first Anti-backflow structure；Second Anti-backflow structure
Micro-fluidic runner 3-13；
Fig. 4 b are the structural schematic diagrams of the lower layer chip of another structure type；
Fig. 5 is the structural schematic diagram of micro-valve of the present invention；
In figure：Upper layer chip 1；Middle level chip 2；Lower layer chip 3；Push-down head 4；Detection probe 5；Conductive seal circle 11；
Fig. 6 is the structural schematic diagram of conductive seal circle of the present invention；
Fig. 7 is the sectional view of conductive seal circle of the present invention；
In Fig. 6-7：Outer layer sealing ring 11-1；Internal close membrane 11-2；Capacitance probe contact 11-3；
Fig. 8 is the structural schematic diagram of the first caulking groove on middle level chip of the present invention；
Fig. 9 is the sectional view of Fig. 8；
In Fig. 8-Fig. 9：Middle level chip 2；First caulking groove 2-1；Micro-valve runner 2-1-1；
Figure 10 is the structural schematic diagram of three-chip type Anti-backflow structure；
Figure 11 is the sectional view of Figure 10；
In Figure 10-11：Upper layer chip 1；Middle level chip 2；Anti-return liquid outlet 2-a；Anti-return is connected to runner 2-b；Prevent back
Flow convex block 2-c；Lower layer chip 3；Micro-fluidic runner 3-a；
Figure 12 is the structural schematic diagram of second of Anti-backflow structure；
Figure 13 is the structural schematic diagram of middle level chip in Figure 12；
In Figure 12-13：Middle level chip 2；Anti-return runner 2-d；Lower layer chip 3；Micro-fluidic runner 3-a；
Figure 14 is the structural schematic diagram of the third Anti-backflow structure；
Figure 15 is the cross section structure schematic diagram of the third Anti-backflow structure described in Figure 14；
Figure 16 is the structural schematic diagram of lower layer chip in Figure 14；
Figure 17 is the structural schematic diagram of middle level chip in Figure 14；
In Figure 14-16：Middle level chip 2；Anti-return runner 2-d；Lower layer chip 3；Micro-fluidic runner 3-a；Micro-fluidic runner
Spacer block 3-b；
Figure 18 is the structural schematic diagram of the 4th kind of Anti-backflow structure；
Figure 19 is the cross section structure schematic diagram of the 4th kind of Anti-backflow structure described in Figure 18；
Figure 20 is the structural schematic diagram of lower layer chip in Figure 18；
Figure 21 is the structural schematic diagram of middle level chip in Figure 18；
In Figure 18-21：Middle level chip 2；Anti-return runner 2-d；Lower layer chip 3；Micro-fluidic runner 3-a；Micro-fluidic runner
Spacer block 3-b；Connecting pole 3-c.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Below
Description only actually at least one exemplary embodiment is illustrative, is never used as to the present invention and its application or makes
Any restrictions.Based on the embodiments of the present invention, those of ordinary skill in the art are not making creative work premise
Lower obtained every other embodiment, shall fall within the protection scope of the present invention.Unless specifically stated otherwise, otherwise in these realities
The positioned opposite of component and step, expression formula and numerical value described in example is applied not limit the scope of the invention.Meanwhile it should be bright
In vain, for ease of description, the size of attached various pieces shown in the drawings is not to be drawn according to actual proportionate relationship.It is right
Technology, method and apparatus known to person of ordinary skill in the relevant may be not discussed in detail, but in the appropriate case, institute
Stating technology, method and apparatus should be considered as authorizing part of specification.In shown here and discussion all examples, appoint
What occurrence should be construed as merely illustrative, not as limitation.Therefore, the other examples of exemplary embodiment can
With with different values.
For ease of description, herein can with use space relative terms, as " ... on ", " in ... top ",
" ... upper surface ", " above " etc., for describing such as a device shown in the figure or feature and other devices or spy
The spatial relation of sign.It should be understood that spatially relative term is intended to comprising the orientation in addition to device described in figure
Except different direction in use or operation.For example, if the device in attached drawing is squeezed, it is described as " in other devices
It will be positioned as " under other devices or construction after part or construction top " or the device of " on other devices or construction "
Side " or " under other devices or construction ".Thus, exemplary term " ... top " may include " ... top " and
" in ... lower section " two kinds of orientation.The device can also other different modes positioning (being rotated by 90 ° or be in other orientation).
As shown in Fig. 1-3, Fig. 4 a, Fig. 4 b, the micro-fluidic chip of the present invention based on the flowing of active control liquid, packet
Three layers of chip are included, upper layer chip 1, middle level chip 2 and lower layer chip 3 is followed successively by from top to bottom, passes through two-by-two in three layers of chip
The mode grafting of groove-pillar is integral, and is bonded by technologies such as ultrasound or laser；Between middle level chip 2 and lower layer chip 3
Form sample introduction chamber, quantitative chamber (can be set as therefore being denoted as sample mark with the chamber of label substance markers sample simultaneously
Note-quantitative chamber), fluid path runner, mixing chamber, reaction chamber, waste chamber and micro-fluidic runner；Wherein：
The sample introduction chamber is used to prepare simultaneously stored sample；Including sample inlet pool 3-1, sample inlet pool 3-1 is to be set to lower layer's core
The Whole Blood Filtration module of piece 3, in octagonal fan-shaped setting；Be fitted with hemofiltration film 10 in Whole Blood Filtration module, and by being set in
Cover board sealing-in on layer chip 2 forms sample introduction chamber；Loading slot is offered on cover board；Sample introduction chamber is set at the position of loading slot
It is equipped with air-path interface 2-8, loading slot is located at the inner cavity of air-path interface 2-8, and air-path interface and the external gas across upper layer chip 1
Road interface 7 connects；Loading slot relative to air-path interface the center of circle in eccentric or be arranged concentrically, the shape of loading slot be it is round or
Person's ellipse or a line are in the rectangular of circular sliding slopes.
The sample marks-quantify chamber, according to own volume size quantitatively receive sample introduction chamber convey come sample,
Sample exports after being reacted with dissolved marker；Therefore, sample of the present invention mark-quantify chamber, can realize
Really quantitatively be loaded blood plasma, mold is quantitative, avoid pipettor in time calibration caused by sample pipetting volume error；Including sample
The quantitative pond 3-2 of processing-, is set to lower layer chip, by forming chamber with middle level chip sealing.
The mixing chamber, the sample blending for sample being marked-being quantified chamber export；Including mixing slot, mixing slot
It is set to lower layer chip 3, the setting of rounded or diamond shape；When mixing slot is at circle, it is in the miniflow flow control of mixing slot both sides
The section in road is at circle；When mixing slot diamondwise is arranged, the micro-fluidic runner in mixing slot both sides is Z-shaped, and Z-shaped miniflow
The section of flow control road 3-3 can with diamond shape mixing slot natural sparse model, i.e., Z-shaped micro-fluidic runner 3-3 can with diamond shape mixing slot from
So connection.There are two mixing slots in attached drawing, respectively：First mixing slot 3-5, the second mixing slot 3-6；Therefore, by with middle level
The cooperation of chip, the mixing chamber that can be formed include two, respectively flow to sequentially connected first mixing chamber according to fluid
Room, the second mixing chamber, micro-fluidic sprue, the first mixing chamber and the second mixing chamber of the first mixing chamber liquid feeding end
The micro-fluidic sprue of micro-fluidic sprue and the second mixing chamber outlet end between room has part that z-shape miniflow is arranged
Flow control road equally has mixed flow effect.
The reaction chamber is embedded with the coating body 9 to react with marker, is exported to waste liquid chamber after the completion of reaction
Room；Including reactive tank, reactive tank is set to lower layer chip 3, pre- to embed the relevant coated antibody of detection project or antigen etc., with mark
Remember the generations reaction such as respective markers object antibody or antigen in object slot.Wherein coating body 9 can also may be used in reaction chamber lower plane
In upper plane.
The fluid path runner 3-4, each chamber for cleaning micro-fluidic runner and on micro-fluidic runner, one end
It is connected to external fluid path interface 6, the other end is then connected to micro-fluidic runner.Fluid path runner 3-4 is in the position of middle level chip 2
Place is provided with the fluid path interface 2-6 of connection, is connect with the external fluid path interface 6 across upper layer chip 1.
The micro-fluidic runner is two-way, respectively micro-fluidic sprue and micro-fluidic from the punishment of sample introduction chamber liquid outlet
Bypass runner；
Sample introduction chamber is marked-is quantified successively chamber with sample by micro-fluidic sprue, mixing chamber, reaction chamber, gives up
Liquid chamber, while sample introduction chamber is connected to by micro-fluidic bypass runner with waste chamber；Waste chamber, including waste liquid tank 3-
8, it is set to lower layer chip 3, wherein putting has blotting paper 8.
The first micro-valve is installed on micro-fluidic bypass runner between sample introduction chamber and waste chamber；Sample introduction chamber and sample
The first anti-return runner is installed on micro-fluidic sprue between the quantitative chamber of label-；Sample marks-quantify chamber and mixing
The second micro-valve is installed on micro-fluidic sprue between chamber；On micro-fluidic sprue between mixing chamber and reaction chamber
It is disposed with the second Anti-backflow structure at the inlet position of reaction chamber, and between reaction chamber and waste chamber
Third micro-valve is installed on micro-fluidic sprue.It is quantitative under the cooperation of the first micro-valve, the second micro-valve with the first Anti-backflow structure
Chamber can be according to own volume size to reaction chamber weight feed sample.
In the present invention, the first micro-valve, the second micro-valve, third micro-valve are the anti-return micro-valve with anti-return function；Figure
The structure of anti-return micro-valve of the present invention is disclosed in detail in 5- Fig. 9, and the anti-return is explained in detail below with reference to attached drawing
The composition of micro-valve：The anti-return micro-valve；Including press mechanism, testing agency, sealing element and micro-valve Anti-backflow structure；Its
In：
The press mechanism, including push-down head 4, the top of 4 internally positioned close membrane 11-2 of the push-down head.
The testing agency, including detection probe 5, the detection probe 5 are connected with push-down head 4, and are touched positioned at probe
The top of point, while between being smaller than between push-down head 4 and internal close membrane 11-2 between detection probe 5 and probe contacts
Away from.The detection probe 5 that the present invention uses is capacitance probe.
The sealing element is conductive seal circle 11, including outer layer sealing ring 11-1, inside close membrane 11-2 and probe
Contact；Internal close membrane 11-2 is in the inner ring of outer layer sealing ring 11-1, and probe contacts are capacitance probe contact 11-3, and outer
Layer sealing ring 11-1 connections.
The micro-valve Anti-backflow structure, the micro-fluidic runner split settings with anti-return micro-valve installed position, including pass through
Logical micro-valve anti-return runner；
The micro-fluidic runner of the anti-return micro-valve installed position is divided into micro-valve liquid input section by micro-fluidic runner tabula
Go out liquid section with micro-valve；Micro-valve liquid input section has liquid outlet, and micro-valve, which goes out liquid section, has inlet；
The lower end of micro-valve anti-return runner is placed in the top of micro-fluidic runner tabula, and micro-valve anti-return runner can be distinguished
Go out with the liquid outlet of micro-valve liquid input section, micro-valve the inlet of liquid section it is corresponding connection；
In the present invention, the inner cavity of micro-valve anti-return runner has micro-valve anti-return convex block；The outer ring of micro-valve anti-return convex block
The sealing ring caulking groove for installing outer layer sealing ring 11-1 is formed between the inner cavity of micro-valve anti-return runner；Micro-valve anti-return is convex
Block tool goes out liquid stream road there are two runner, respectively micro-valve anti-return feed liquor runner, micro-valve anti-return is penetrated through；Micro-valve anti-return feed liquor
The lower end of runner is connected to the liquid outlet of micro-valve liquid input section, and the lower end that micro-valve anti-return goes out liquid stream road then goes out liquid section with micro-valve
Inlet is connected to；Outer layer sealing ring 11-1 is inlaid in sealing ring caulking groove, and internal close membrane 11-2 can cover micro-valve simultaneously
The upper end of anti-return feed liquor runner, micro-valve anti-return go out the upper end in liquid stream road；
When the present invention not equipped Anti-backflow structure, the inside close membrane 11-2 can cover micro-valve feed liquor simultaneously
The liquid outlet of section, micro-valve go out the inlet of liquid section, and are sealed by outer layer sealing ring 11-1.
The detection probe 5 of testing agency is touched with the pressing action of press mechanism with capacitance probe contact 11-3；At any time
Whether detection liquid flows in valve.At this point, anti-return micro-valve is in the open state.In fact, in the push-down head 4 of press mechanism
Between internal close membrane 11-2 there are spacing in the case of, due to the internal close membrane 11-2 deformation having the function of itself and
The deformation space that installed position has, causes under the drive of air-flow, and fluid can overcome internal envelope from micro-valve liquid input section
After closing film 11-2 deformation, go out liquid section into micro-valve.
When connecting gas circuit, under the air pressure driving that gas circuit provides, liquid moves forward, the fluid warp in micro-valve liquid input section
After micro-valve anti-return runner, it flow to micro-valve and goes out liquid section, the capacitance probe in testing agency detects that liquid flow comes, again
Press mechanism is triggered, under the drive for pushing driving, internal close membrane 11-2 seals the upper end of micro-valve anti-return runner, at this point,
Anti-return micro-valve is closed；
Testing agency includes capacitance probe, and capacitance probe, can be with capacitance under the drive of the pushing driving of press mechanism
Probe contacts 11-3 is electrically connected.
Specifically：When the push-down head 4 of press mechanism with detection probe 5 towards conductive seal 11 downlink of circle when, detecting
When probe 5 is touched with probe contacts, press mechanism stops downlink, at this point, between existing between push-down head 4 and internal close membrane 11-2
Away from；When detection probe 5 sense data change, show that there are the miniflow flow controls that fluid flows through valving installed position
When road, triggering press mechanism action pushes push-down head 4 to continue traveling downwardly, until push-down head 4 pushes down internal close membrane 11-2, with same
When seal the liquid outlet of micro-valve liquid input section, micro-valve goes out the inlet of liquid section, valving is in current floe condition at this time；Instantly press
There are when gap between the push-down head 4 of structure and internal close membrane 11-2, valving is in the open state, internal close membrane 11-2
Possessed deformation characteristics, it is ensured that fluid can be flowed out from the liquid outlet of micro-valve liquid input section, and the inlet stream of liquid section is gone out from micro-valve
Enter.
In attached drawing, the valving be three-chip type structure, including be sequentially distributed from top to bottom upper layer chip 1, middle level
Chip 2 and next chip；Upper layer chip 1, middle level chip 2 and next chip are mutually clamped connection two-by-two；Micro-valve anti-return
For structure setting in middle level chip 2, micro-fluidic runner is arranged in lower layer chip 3, and caulking groove is set to corresponding to micro-valve Anti-backflow structure
The upper surface of middle level chip 2, sealing element are pressed on by upper layer chip 1 in caulking groove, and upper layer chip 1 corresponds to inner sealing
Circle, the position for detecting contact accordingly open up pressure head and walk hole, pop one's head in and walk hole respectively；And press mechanism is located at upper layer chip 1
Top, press mechanism pushes, drives push-down head 4 to walk hole by pressure head, push down internal close membrane 11-2, anti-to seal micro-valve
The upper end of reflux runner, at this point, anti-return micro-valve is closed；Capacitance probe is with the pushing of press mechanism and towards upper
Layer chip 1 moves, after walking hole up through the probe on upper layer chip 1, with the conductive seal being placed in 2 caulking groove of middle level chip
The probe contacts of circle 11 touch, and realize and are electrically connected.
The present invention creatively devises valving, makes itself and the control back-flow preventer fusion of micro-fluidic liquid one
It rises, saves micro-fluidic chip space.The locational space of micro-fluidic chip is extremely limited, and the present invention can effectively save miniflow flow control
Road space, it is more efficient when can make design runner.Avoid between each runner flow channel for liquids closing caused by hypotelorism not
Sternly, the problem of percolation road.
The present invention utilizes the electric conductivity of conductive rubber material, the capacitance detecting probe 5 of bonding apparatus to observe liquid in runner
It is flow to the exact moment of valve, closes valve in time.Save semipermeable membrane device.
In Figure 10-Figure 21, it is disclosed in detail involved by the first Anti-backflow structure of the present invention, the second Anti-backflow structure
And the concrete structure of embodiment, explain the corresponding Anti-backflow structure of each embodiment of the present invention in detail below with reference to attached drawing
Specific composition, in addition, for convenience of describe, the first Anti-backflow structure, the second Anti-backflow structure are equal to anti-return below
Structure.
According to each embodiment it is found that Anti-backflow structure of the present invention, has the property that：
There is Anti-backflow structure anti-return runner, anti-return runner can be lifted the miniflow of Anti-backflow structure installed position
Flow control road liquid level；
When connecting gas circuit, under the air pressure driving that gas circuit provides, anti-return runner is overcome to be lifted micro-fluidic herein
The pressure of runner liquid level promotes the fluid in the micro-fluidic runner of Anti-backflow structure both sides to be in circulation status.
Embodiment 1
As illustrated by figs. 12-13, the Anti-backflow structure described in the present embodiment, including anti-return runner 2-d (are set to middle level core
Piece 2), extending direction and the micro-fluidic runner 3-a of Anti-backflow structure installed position of the anti-return runner 2-d (are set to down
Layer chip) extending direction it is perpendicular；The micro-fluidic runner 3-a of Anti-backflow structure installed position passes through micro-fluidic runner spacer block
3-b is separated into two sections, respectively micro-fluidic feed liquor runner, it is micro-fluidic go out liquid stream road；The upper end closed of anti-return runner 2-d, under
End is then connected across above micro-fluidic runner spacer block 3-b, and respectively with micro-fluidic feed liquor runner, it is micro-fluidic go out liquid stream road be connected to；When
When connecting gas circuit, under the air pressure driving that gas circuit provides, after the fluid in micro-fluidic feed liquor runner flows through anti-return runner 2-d,
Be back to it is micro-fluidic go out liquid stream road in.
Anti-backflow structure described in the present embodiment has a single function, and effect is not notable.The height and miniflow flow control of Anti-backflow structure
Liquid surface height is consistent in road 3-a, does not play the purpose of complete anti-return, but simple in structure, and production requirement is not high.
Embodiment 2
As shown in figs. 10-11, Anti-backflow structure described in the present embodiment difference from example 1 is that：
1, there is anti-return convex block 2-c, and anti-return convex block 2-c and anti-return runner 2-d in the anti-return runner 2-d
Between be formed with two anti-returns connection runner 2-b；Two anti-returns are connected to runner 2-b, respectively with micro-fluidic feed liquor
Runner, it is micro-fluidic go out liquid stream road connection；The lower end of anti-return convex block 2-c is flushed with the lower end of anti-return runner 2-d, and anti-return
The upper end that the upper end of convex block 2-c is then less than anti-return runner 2-d is arranged.
2, the upper end closed of the anti-return runner 2-d is that the cover board provided by upper layer chip acts on to complete.
It follows that the raised back-flow preventer on micro-fluidic liquid surface that the present embodiment is creative, can significantly rise
To the effect of liquid anti-return.Requirement simultaneously to production technology is less high, achievees the purpose that large-scale production.
Embodiment 3
As shown in figures 14-17, Anti-backflow structure described in the present embodiment and embodiment 2 the difference is that：
Set the micro-fluidic runner spacer block 3-b to wedge block, and anti-return runner 2-d is shape and micro-fluidic runner
The similar wedge groove of spacer block 3-b shapes.
It can thus be appreciated that：Due to the raised runner of the present embodiment, it can significantly play the role of anti-return.
Embodiment 4
As shown in figs. 18-21, Anti-backflow structure described in the present embodiment and embodiment 3 the difference is that：
The micro-fluidic runner 3-a of Anti-backflow structure installed position, in the both sides of micro-fluidic runner spacer block 3-b symmetrically cloth
It is equipped with wedge-shaped connecting pole 3-c, anti-return runner 2-d has the wedge-shaped link slot being connected with wedge-shaped connecting pole 3-c.
It can thus be appreciated that：The present embodiment creatively devises back-flow preventer, makes itself and micro-fluidic chip fixed bit up and down
Pillar be merged (embodiment 3 is all boss, but due to size be less than 500 microns, can not be as fixed bit
With insufficient strength), save micro-fluidic chip space.Simultaneously because bifacial protrusion enclosure wall, effectively avoids liquid from side
Face leaks out, and influences anti-return.
In addition, above-mentioned Anti-backflow structure, can also be applied to micro-valve, only in micro-valve structure, Anti-backflow structure is anti-
The closing of reflux runner upper end, is realized by internal close membrane.
The present invention can control back-flow preventer by micro-fluidic liquid, and an equipment is allow to operate multiple micro-fluidic cores
Piece plays the effect of more flux, even if because the motive force of liquid flow forward is removed, liquid will not flow back over, because
To there is back-flow preventer, when incubation time between step each in this way, equipment may exit off micro-fluidic chip, go to operate another
A micro-fluidic chip plays the purpose of more flux.
In addition, for convenience of the use of micro-fluidic chip of the present invention, it is provided with for external in the upper layer chip
Fixed part；The external fixed part is spherical shape fixed part either groove-like fixed part or the end being set on the outside of the chip of upper layer
Portion's card slot fixed part.
Claims (10)
1. it is a kind of based on active control liquid flowing micro-fluidic chip, including sample introduction chamber, reaction chamber, waste chamber and
Micro-fluidic runner, sample introduction chamber are connected to gas circuit；It is characterized in that：It is equipped in normally open on the micro-fluidic runner
Micro-valve, the micro-valve have prevent the micro-valve Anti-backflow structure of fluid reflux and for sensing whether fluid flows through micro-valve installation position
The testing agency set；
Micro-valve in normally open can when the numerical tabular free flow body that testing agency is fed back flows through micro-valve installation site
It is automatic to be closed, it is in current floe condition；
Micro-valve in current floe condition can be opened periodically, and circulation status is in；
Micro-valve in circulation status, in the case where the air-flow that gas circuit provides pushes, fluid can circulate in micro-valve.
2. the micro-fluidic chip according to claim 1 based on the flowing of active control liquid, it is characterised in that：The micro-valve
Including the first micro-valve, the second micro-valve；It is provided with quantitative chamber between the sample introduction chamber and reaction chamber；
Micro-fluidic runner at sample introduction chamber liquid outlet is divided into two-way, respectively micro-fluidic sprue and micro-fluidic bypass runner；
Sample introduction chamber is connected to quantitative chamber, reaction chamber, waste chamber successively by micro-fluidic sprue, while sample introduction chamber passes through
Micro-fluidic bypass runner is connected to waste chamber；
The first micro-valve is installed on micro-fluidic bypass runner between sample introduction chamber and waste chamber；
The second micro-valve is installed on the micro-fluidic sprue of the liquid outlet of quantitative chamber；
The first Anti-backflow structure is installed on micro-fluidic sprue between sample introduction chamber and quantitative chamber；
The first micro-valve, the second micro-valve, the first Anti-backflow structure cooperation under, quantitative chamber can according to own volume size to
Reaction chamber weight feed sample.
3. the micro-fluidic chip according to claim 2 based on the flowing of active control liquid, it is characterised in that：It is described quantitative
There is evenly mixing device, which includes mixing chamber and mixing runner between chamber and reaction chamber；Mixing runner is in Z
Shape；The micro-fluidic sprue of mixing chamber both sides is partly at least mixing runner, and mixing chamber can be with mixing runner
Natural communication.
4. the micro-fluidic chip according to claim 2 based on the flowing of active control liquid, it is characterised in that：
There is first Anti-backflow structure anti-return runner, anti-return runner can be lifted the first Anti-backflow structure installation site
The micro-fluidic runner liquid level at place；
The extending direction of the micro-fluidic runner of the extending direction of the anti-return runner and the first Anti-backflow structure installed position
It is perpendicular；
The micro-fluidic runner of first Anti-backflow structure installed position is separated into two sections by micro-fluidic runner spacer block, respectively micro-
Flow control feed liquor runner, it is micro-fluidic go out liquid stream road；
There is anti-return convex block in the anti-return runner, and be formed with two between anti-return convex block and anti-return runner and prevent back
Stream connection runner；
Two anti-returns are connected to runner, respectively with micro-fluidic feed liquor runner, it is micro-fluidic go out liquid stream road be connected to；
The lower end of anti-return convex block is flushed with the lower end of anti-return runner, and the upper end of anti-return convex block is then less than anti-return runner
Upper end setting；
When connecting gas circuit, under the air pressure driving that gas circuit provides, the runner micro-fluidic herein that overcomes anti-return runner to be lifted
The pressure of liquid level promotes the fluid in the micro-fluidic runner of the first Anti-backflow structure both sides to be in circulation status.
5. the micro-fluidic chip according to claim 4 based on the flowing of active control liquid, it is characterised in that：The miniflow
Flow control road spacer block is wedge block, and anti-return runner is shape wedge groove similar with micro-fluidic runner spacer block shape；First is anti-
The micro-fluidic runner of return-flow structure installed position has been arranged symmetrically wedge-shaped connecting pole in the both sides of micro-fluidic runner spacer block,
Anti-return runner has the wedge-shaped link slot being connected with wedge-shaped connecting pole.
6. the micro-fluidic chip according to claim 1 based on the flowing of active control liquid, it is characterised in that：
Micro-valve further includes press mechanism, sealing element；Wherein：
The micro-valve Anti-backflow structure, the micro-fluidic runner split settings with anti-return micro-valve installed position, including perforation
Micro-valve anti-return runner；
The micro-fluidic runner of the anti-return micro-valve installed position is divided into micro-valve liquid input section and micro- by micro-fluidic runner tabula
Valve goes out liquid section；Micro-valve liquid input section has liquid outlet, and micro-valve, which goes out liquid section, has inlet；
The lower end of micro-valve anti-return runner is placed in the top of micro-fluidic runner tabula, and micro-valve anti-return runner can respectively with it is micro-
The inlet that the liquid outlet of valve liquid input section, micro-valve go out liquid section corresponds to connection；
The upper end of micro-valve anti-return runner has the caulking groove for installing sealing element；
The sealing element is conductive seal circle, including outer layer sealing ring, internal close membrane and capacitance probe contact；Inside envelope
The inner ring that film is in outer layer sealing ring is closed, and capacitance probe contact is then connect with outer layer sealing ring；Sealing element is flush-mounted in caulking groove,
And internal close membrane covers the upper end of micro-valve anti-return runner just, while internal close membrane has the space of free deformation；
The press mechanism, including push-down head, the top of the internally positioned close membrane of the push-down head；
The testing agency, including detection probe, the detection probe are connected with push-down head, and positioned at the upper of probe contacts
Side, while the spacing being smaller than between push-down head and internal close membrane between detection probe and probe contacts；
When the push-down head of press mechanism with detection probe towards conductive seal circle downlink when, in detection probe and probe contacts phase
When touching, press mechanism stops downlink, at this point, there are spacing between push-down head and internal close membrane；When the number of detection probe sensing
According to changing, show that triggering press mechanism acts there are when the micro-fluidic runner that fluid flows through valving installed position,
Push-down head is pushed to continue traveling downwardly, until push-down head pushes down internal close membrane, to seal liquid outlet, the micro-valve of micro-valve liquid input section simultaneously
Go out the inlet of liquid section, valving is in current floe condition at this time；
When, there are when gap, valving is in the open state, and inside is sealed between the push-down head of press mechanism and internal close membrane
Close deformation characteristics possessed by film, it is ensured that fluid can be flowed out from the liquid outlet of micro-valve liquid input section, and the feed liquor of liquid section is gone out from micro-valve
Mouth flows into.
7. the micro-fluidic chip according to claim 2 based on the flowing of active control liquid, it is characterised in that：The reaction
Third micro-valve is installed on micro-fluidic sprue between chamber and waste chamber；In close proximity to the miniflow of reaction chamber inlet
The second Anti-backflow structure is installed on control sprue；Third micro-valve is the anti-return micro-valve with anti-return function.
8. a kind of micro-fluidic chip based on the flowing of active control liquid, it is characterised in that：Including sample introduction chamber, sample label-
Quantitative chamber, fluid path runner, mixing chamber, reaction chamber, waste chamber and micro-fluidic runner；Wherein：
The sample introduction chamber is used to prepare simultaneously filtered sample；
The sample marks-quantify chamber, according to own volume size quantitatively receive sample introduction chamber convey come sample, and will
It is exported after sample and marker reaction；
The mixing chamber, the sample blending for sample being marked-being quantified chamber export；
The reaction chamber is embedded with the coating body to react with marker, is exported to waste chamber after the completion of reaction；
Fluid path runner, each chamber for cleaning micro-fluidic runner and on micro-fluidic runner, one end and external fluid path
Orifice, the other end are then connected to micro-fluidic runner；
Micro-fluidic runner includes micro-fluidic sprue and micro-fluidic bypass runner；
Sample introduction chamber marks-quantifies successively chamber, mixing chamber, reaction chamber, waste liquid chamber with sample by micro-fluidic sprue
Room is connected to, while sample introduction chamber is connected to by micro-fluidic bypass runner with waste chamber；
The first micro-valve is installed on micro-fluidic bypass runner between sample introduction chamber and waste chamber；Sample introduction chamber and sample mark
The first Anti-backflow structure is installed on micro-fluidic sprue between the quantitative chamber of note-；Sample marks-quantifies chamber and mixing chamber
The second micro-valve is installed on micro-fluidic sprue between room；On micro-fluidic sprue between mixing chamber and reaction chamber
It nestles up and is disposed with the second Anti-backflow structure at the inlet position of reaction chamber, and it is micro- between reaction chamber and waste chamber
Third micro-valve is installed on flow control sprue；
First Anti-backflow structure, the second Anti-backflow structure include anti-return runner；
The extending direction of the anti-return runner and the extending direction of the micro-fluidic runner of Anti-backflow structure installed position are perpendicular；
The micro-fluidic runner of Anti-backflow structure installed position is separated into two sections by micro-fluidic runner spacer block, respectively micro-fluidic
Feed liquor runner, it is micro-fluidic go out liquid stream road；
The upper end closed of anti-return runner, lower end are then connected across above micro-fluidic runner spacer block, and respectively with micro-fluidic into liquid stream
Road, it is micro-fluidic go out liquid stream road connection；
When connecting gas circuit, under the air pressure driving that gas circuit provides, the fluid in micro-fluidic feed liquor runner flows through anti-return runner
Afterwards, be back to it is micro-fluidic go out liquid stream road in；
First micro-valve, the second micro-valve, third micro-valve are anti-return micro-valve, including press mechanism, testing agency, sealing element
And micro-valve Anti-backflow structure；Wherein：
The micro-valve Anti-backflow structure, the micro-fluidic runner split settings with anti-return micro-valve installed position, including perforation
Micro-valve anti-return runner；
The micro-fluidic runner of the anti-return micro-valve installed position is divided into micro-valve liquid input section and micro- by micro-fluidic runner tabula
Valve goes out liquid section；Micro-valve liquid input section has liquid outlet, and micro-valve, which goes out liquid section, has inlet；
The lower end of micro-valve anti-return runner is placed in the top of micro-fluidic runner tabula, and micro-valve anti-return runner can respectively with it is micro-
The inlet that the liquid outlet of valve liquid input section, micro-valve go out liquid section corresponds to connection；
The upper end of micro-valve anti-return runner has the caulking groove for installing sealing element；
The sealing element is conductive seal circle, including outer layer sealing ring, internal close membrane and capacitance probe contact；Inside envelope
The inner ring that film is in outer layer sealing ring is closed, and capacitance probe contact is then connect with outer layer sealing ring；Sealing element is flush-mounted in caulking groove,
And internal close membrane covers the upper end of micro-valve anti-return runner just, while internal close membrane has the space of free deformation；
The press mechanism, including push-down head, the top of the internally positioned close membrane of the push-down head；
The testing agency, including detection probe, the detection probe are connected with push-down head, and positioned at the upper of probe contacts
Side, while the spacing being smaller than between push-down head and internal close membrane between detection probe and probe contacts；
When the push-down head of press mechanism with detection probe towards conductive seal circle downlink when, in detection probe and probe contacts phase
When touching, press mechanism stops downlink, at this point, there are spacing between push-down head and internal close membrane；When the number of detection probe sensing
According to changing, show that triggering press mechanism acts there are when the micro-fluidic runner that fluid flows through valving installed position,
Push-down head is pushed to continue traveling downwardly, until push-down head pushes down internal close membrane, to seal liquid outlet, the micro-valve of micro-valve liquid input section simultaneously
Go out the inlet of liquid section, valving is in current floe condition at this time；
When, there are when gap, valving is in the open state, and inside is sealed between the push-down head of press mechanism and internal close membrane
Close deformation characteristics possessed by film, it is ensured that fluid can be flowed out from the liquid outlet of micro-valve liquid input section, and the feed liquor of liquid section is gone out from micro-valve
Mouth flows into.
9. the micro-fluidic chip according to claim 8 based on the flowing of active control liquid, it is characterised in that：The mixing
Chamber includes two, respectively flows to sequentially connected first mixing chamber, the second mixing chamber according to fluid, and described first is mixed
Micro-fluidic sprue between the micro-fluidic sprue of even chamber liquid feeding end, the first mixing chamber and the second mixing chamber and
The micro-fluidic sprue of two mixing chamber outlet ends has part that z-shape mixed flow runner is arranged.
10. the micro-fluidic chip according to claim 9 based on the flowing of active control liquid, it is characterised in that：It is described micro-
Fluidic chip includes three layers of chip, is followed successively by upper layer chip, middle level chip and lower layer chip from top to bottom, two in three layers of chip
Two by way of groove-pillar grafting it is integral；Sample introduction chamber, sample label-are formed between middle level chip and lower layer chip
Quantitative chamber, fluid path runner, mixing chamber, reaction chamber, waste chamber and micro-fluidic runner；And sample introduction chamber is in
The loading slot of connection is provided at the position of layer chip, and sample introduction chamber is provided with air-path interface at the position of loading slot, adds
Sample slot is located at the inner cavity of air-path interface, and air-path interface is connect with the external air-path interface across upper layer chip；Fluid path runner exists
It is provided with the fluid path interface of connection at the position of middle level chip, is connect with the external fluid path interface across upper layer chip；
The sample introduction chamber includes sample inlet pool, and sample inlet pool is the Whole Blood Filtration module for being set to lower layer chip, is set in octagonal sector
It sets；Hemofiltration film is fitted in Whole Blood Filtration module, and the cover board sealing-in by being set on the chip of middle level forms sample introduction chamber；Lid
Loading slot is offered on plate；Loading slot in bias or is arranged concentrically relative to the center of circle of air-path interface, and the shape of loading slot is
Round either ellipse or a line are in the rectangular of circular sliding slopes；
Mixing chamber includes mixing slot, and mixing slot is set to lower layer chip, the setting of rounded or diamond shape；When mixing slot is at circle
When, the section of the micro-fluidic runner in mixing slot both sides is at circle；When mixing slot diamondwise is arranged, it is in mixing slot both sides
Micro-fluidic runner be Z-shaped, and the section of Z-shaped micro-fluidic runner can be with diamond shape mixing slot natural sparse model.
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| CN201710213382.XA CN106955750B (en) | 2017-04-01 | 2017-04-01 | Micro-fluidic chip based on the flowing of active control liquid |
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| CN117244600B (en) * | 2023-11-15 | 2024-02-13 | 至美时代生物智能科技(北京)有限公司 | Reaction chamber, reaction chamber group and micro-fluidic chip |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001088525A1 (en) * | 2000-05-12 | 2001-11-22 | University Of Cincinnati | Structurally programmable microfluidic systems |
| US7323140B2 (en) * | 2001-03-28 | 2008-01-29 | Handylab, Inc. | Moving microdroplets in a microfluidic device |
| CN104911095B (en) * | 2015-06-16 | 2017-07-07 | 东南大学 | A kind of fluid cartridge |
| CN205517817U (en) * | 2016-03-31 | 2016-08-31 | 苏州市博纳泰科生物技术有限公司 | Prevent little valve of refluence |
| CN105879936B (en) * | 2016-03-31 | 2017-10-13 | 张鹏 | Whole Blood Filtration and quantitatively pipette micro-fluidic chip |
| CN206868242U (en) * | 2017-04-01 | 2018-01-12 | 南京岚煜生物科技有限公司 | Micro-fluidic chip based on the flowing of active control liquid |
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