CN106834110A - A kind of isothermal PCR detector and its detection method - Google Patents
A kind of isothermal PCR detector and its detection method Download PDFInfo
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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Abstract
The invention discloses a kind of isothermal PCR detector and its detection method, isothermal PCR detector includes：Power module, raising module, one or more detection modules, ultraviolet irradiation module, optical process modules, light signal collection module, CPU control computation analysis module and human-computer interaction module, the present invention mainly solves the problems, such as that isothermal PCR instrument detection speed is slow by the design of multichannel, the problem that sample incubation and detection can be completed in the same apparatus is realized by the integrated raising module on detector, testing result is realized by advanced optical pickocff and perfect algorithm and is quantitatively calculated.
Description
Technical field
The present invention relates to a kind of isothermal PCR detector and its detection method.
Background technology
At present, sentencing for testing result is carried out using macroscopic method more than isothermal PCR detector
It is fixed, and the incubation and detection of most equipment need separately to carry out in existing equipment, this equipment
Incubation and detection in same equipment complete, centre does not need manual operation.
Macroscopic method is although simple and clear, but macroscopic method can only accomplish fixed
Property analysis, it is impossible to complete quantitative analysis, and macroscopic method is influenceed by individual's level
Than more serious, it is impossible to the truth of accurate and visual response measurement result.Additionally, due to present
Equipment in it is most using being incubated and by the way of detection is separated, so inconvenient scene is quickly examined
Survey.
The content of the invention
In order to solve the above problems, the invention provides a kind of isothermal PCR detector, including：
Power module, raising module, one or more detection modules, ultraviolet irradiation module, optics
Processing module, light signal collection module, CPU control computation analysis module and human-computer interaction module,
Power module be respectively connecting to raising module, ultraviolet irradiation module, light signal collection module,
CPU controls computation analysis module and human-computer interaction module, CPU control computation analysis module difference
It is connected to power module, raising module, ultraviolet irradiation module, light signal collection module and people
Machine interactive module, one or more detection modules are respectively positioned at the 12V resistance heatings of raising module
In one or more constant-temperature incubation spaces that film is surrounded, ultraviolet irradiation module and light signal collection
Module is located at below one or more detection modules and side respectively, and optical process modules are located at
Between ultraviolet irradiation module and one or more detection modules or light signal collection module and one
Between individual or multiple detection modules.
According to a kind of above-mentioned isothermal PCR detector, wherein, in one or more detection modules
Each detection module is provided with one or more groups of detection holes, and every group of detection hole includes multiple detection
Hole, wherein, one or more detection modules are one or more aluminium blocks.
According to a kind of above-mentioned isothermal PCR detector, wherein, ultraviolet irradiation module includes multiple
LED light source, multiple LED light sources are separately positioned on each inspection on one or more detection modules
Below gaging hole.
According to a kind of above-mentioned isothermal PCR detector, wherein, light signal collection module includes multiple
Light signal acquisition device, multiple light signal acquisition devices are separately positioned on one or more detection moulds
The side in each the detection hole on block.
According to a kind of above-mentioned isothermal PCR detector, wherein, optical process modules enter including multiple
Penetrate optical processor and multiple excites optical processor, between each LED light source and corresponding detection hole
It is provided with an incident optical processor, and each light signal acquisition device and corresponding detection hole
Between be provided with one and excite optical processor.
According to a kind of above-mentioned isothermal PCR detector, wherein, power module includes the external electricity of 24V
Source adapter and chargeable lithium cell, 24V external power adapters and chargeable lithium cell are distinguished
It is connected with mains 220V power supplys, and 24V external power adapters are through power filter part
The 12V resistance heatings film of raising module is partially attached to or by 5V drops by 12V step-downs afterwards
Laminate section is respectively connecting to CPU controls computation analysis module, the multiple of ultraviolet irradiation module
Multiple light signal acquisition devices and human-computer interaction module of LED light source, light signal collection module；
And chargeable lithium cell is depressured the 12V resistance heating films for being partially attached to raising module by 12V
Or CPU controls computation analysis module, ultraviolet irradiation are respectively connecting to by 5V step-downs part
Multiple LED light sources of module, multiple light signal acquisition devices of light signal collection module and man-machine
Interactive module.
According to a kind of above-mentioned isothermal PCR detector, wherein, chargeable lithium cell is provided with charge and discharge
Electric management module, management of charging and discharging module is connected with CPU controls computation analysis module.
According to a kind of above-mentioned isothermal PCR detector, wherein, raising module is provided with one or many
Individual temperature sensor carries out temperature acquisition, and one or more temperature sensors control to calculate with CPU
Analysis module is connected.
According to a kind of above-mentioned isothermal PCR detector, wherein, human-computer interaction module mainly includes key
Disk, battery capacity indication lamp and digital display screen, the connection of keyboard, battery capacity indication lamp and digital display screen
Computation analysis module is controlled to CPU.
According to a kind of above-mentioned detection method of isothermal PCR detector, including：
Power module, is respectively connecting to raising module, ultraviolet irradiation module, light signal collection
Module, CPU control computation analysis module and human-computer interaction module to be powered for it；
CPU controls computation analysis module, is respectively connecting to power module, raising module, ultraviolet
Line irradiation module, light signal collection module and human-computer interaction module are controlling modules and calculating
Analysis detecting data；
Raising module, one or more constant-temperature incubations for being provided with 12V resistance heatings film to surround
Space, and one or more temperature sensors carry out temperature acquisition, one or more of temperature
Sensor is connected the temperature number for collecting temperature sensor with CPU controls computation analysis module
Control computation analysis module to control the temperature of raising module according to sending to CPU；
One or more detection modules, respectively in one or more constant-temperature incubation spaces, often
Individual detection module is provided with one or more groups of detection holes, and every group of detection hole includes multiple detection holes,
The testing sample that will be handled well is placed in centrifuge tube, and centrifuge tube is placed in detection hole and is detected；
Ultraviolet irradiation module, including multiple LED light sources, are separately positioned on one or more inspections
Below each the detection hole surveyed in module；
Light signal collection module, including multiple light signal acquisition devices, be separately positioned on one or
The side in each the detection hole on multiple detection modules；
Optical process modules, including multiple incidence optical processors and multiple excite optical processor, often
An incident optical processor, and each are provided between individual LED light source and corresponding detection hole
One is provided between light signal acquisition device and corresponding detection hole excite optical processor, this
Sample forms multigroup and multiple sense channels；And
Human-computer interaction module, mainly including keyboard, battery capacity indication lamp and digital display screen, connects
It is connected to CPU and controls computation analysis module to realize man-machine interaction.
Beneficial effect
The invention discloses a kind of isothermal PCR detector and its detection method, there is advantages below：
1st, quantifying for testing result is realized by advanced photoelectric sensor and perfect algorithm
Calculate and analyze.
2nd, testing sample incubation is realized by the method for integrated raising module, detection can not be again
The problem completed in same equipment.
3rd, solve the problems, such as that isothermal PCR instrument detection speed is slow by the design of multichannel.
4th, a kind of isothermal PCR detector disclosed by the invention and its detection method can be to golden yellow
Color staphylococcus, oral cavity streptococcus mutans, helicobacter pylori, Bacillus acidi lactici, legionella pneumophilia,
Control field bend bacterium, Salmonella paratyphi A etc. are detected.
Brief description of the drawings
Fig. 1 is a kind of structural representation of isothermal PCR detector disclosed by the invention；
Fig. 2 is a kind of ultraviolet irradiation of isothermal PCR detector disclosed by the invention, at optics
Reason and light signal collection principle schematic；
Fig. 3 be a kind of power module of isothermal PCR detector disclosed by the invention power supply and its
Control schematic diagram.
Specific embodiment
The present invention is described in further detail with specific embodiment below in conjunction with the accompanying drawings, but is not made
It is limitation of the invention.
Fig. 1 is a kind of structural representation of isothermal PCR detector disclosed by the invention, such as Fig. 1
It is shown, the invention provides a kind of isothermal PCR detector, including：Power module 01, incubate
Educate module 02, one or more detection module (not shown)s, ultraviolet irradiation module
03rd, optical process modules (not shown), light signal collection module 04, CPU control meter
Analysis module 05 and human-computer interaction module 06 are calculated, power module 01 is respectively connecting to be incubated mould
Block 02, ultraviolet irradiation module 03, the control of light signal collection module 04, CPU calculate analysis
Module 05 and human-computer interaction module 06, CPU controls computation analysis module 05 are respectively connecting to electricity
Source module 01, raising module 02, ultraviolet irradiation module 03, light signal collection module 04
With human-computer interaction module 06, CPU control computation analysis module 05 use STM32F4 systems
Row single-chip microcomputer, major function is that each functional module of equipment is controlled, and to collection
Result is analyzed and calculates, and one or more detection modules are respectively positioned at raising module 02
In one or more constant-temperature incubation spaces that 12V resistance heating films are surrounded, ultraviolet irradiation module
03 and light signal collection module 04 respectively be located at one or more detection modules below and side
Face, optical process modules be located at ultraviolet irradiation module 03 and one or more detection modules it
Between or light signal collection module 04 and one or more detection modules between.
Fig. 2 is a kind of ultraviolet irradiation of isothermal PCR detector disclosed by the invention, at optics
Reason and light signal collection principle schematic, as shown in Fig. 2 being examined according to a kind of above-mentioned isothermal PCR
Instrument is surveyed, wherein, each detection module in one or more detection modules is provided with one group or many
Group detection hole, every group of detection hole includes multiple detection holes, wherein, one or more detection modules
It is one or more aluminium blocks, ultraviolet irradiation module includes that multiple LED light sources (do not show in figure
Go out), multiple LED light sources 07 are separately positioned on each inspection on one or more detection modules
Below gaging hole 08, light signal collection module includes multiple light signal acquisition devices 09, multiple
Light signal acquisition device 09 is separately positioned on each the detection hole on one or more detection modules
08 side, optical process modules are included at multiple incidence optical processors 10 and multiple exciting lights
Reason device 11, is provided with one and enters between each LED light source 07 and corresponding detection hole 08
Penetrate between optical processor 10, and each light signal acquisition device 09 and corresponding detection hole 08
It is provided with one and excites optical processor 11.
Incident light uses ultraviolet light in the present invention, and light source is high power ultraviolet LED, is
Preferable fluorescence excitation effect is reached, the present invention uses optical filter, i.e., incident optical processor
The light that the 10 pairs of light sources send is processed, to ensure the unicity of its output light wavelength.Excite
Light aspect, the sample 21 in sample tube 20 is irradiated the exciting light for producing by incident uv
It is green, so needing to swash by exciting optical processor 11 pairs before light signal collection is carried out
It is luminous to be processed, to ensure that light signal collection circuit is not disturbed by other light.Optical signal
Acquisition module 04 includes multiple light signal acquisition devices 09, and its Main Function is by photoelectric transfer
Exciting light is converted into electric signal by sensor, is afterwards turned electric signal by 16 AD conversion chips
Change digital quantity into, there is provided further analyzed to CPU and calculated.
Fig. 3 be a kind of power module of isothermal PCR detector disclosed by the invention power supply and its
Control schematic diagram, as shown in figure 3, according to a kind of above-mentioned isothermal PCR detector, wherein, electricity
Source module 01 includes 24V external power adapters 12 and chargeable lithium cell 13, and 24V is external
Power supply adaptor 12 and chargeable lithium cell 13 are connected with mains 220V power supplys 14 respectively,
And 24V external power adapters 12 are depressured part behind power filter part 15 by 12V
The 16 12V resistance heatings films 17 for being connected to raising module 02 are depressured part 18 by 5V
It is respectively connecting to CPU controls computation analysis module 05, multiple LED of ultraviolet irradiation module 03
Light source 07, multiple light signal acquisition devices 09 of light signal collection module 04 and man-machine interaction
Module 06, the function of power filter part 15 is that to filter electromagnetic interference in external circuitses etc. dry
Disturb composition；And chargeable lithium cell 13 is depressured part 16 and is connected to raising module by 12V
02 12V resistance heatings film 17 is respectively connecting to CPU controls by 5V step-downs part 18
Computation analysis module 05, multiple LED light sources 07, optical signal of ultraviolet irradiation module 03 are adopted
Collect multiple light signal acquisition devices 09 and human-computer interaction module 06 of module 04.
As shown in figure 3, according to a kind of above-mentioned isothermal PCR detector, wherein, chargeable lithium electricity
Pond 13 is provided with management of charging and discharging module 19, and management of charging and discharging module 19 controls meter with CPU
Analysis module 05 is calculated to connect, management of charging and discharging module 19 be responsible for the charging of lithium ion battery and
Electric discharge is managed, and lithium battery is protected, and prevents from breaking through the generation of the problems such as putting.
According to a kind of above-mentioned isothermal PCR detector, wherein, raising module is provided with one or many
Individual temperature sensor carries out temperature acquisition, and one or more temperature sensors control to calculate with CPU
Analysis module connects (not shown).
The function of raising module 02 is that the sample to handling well is incubated, and is used in the design
Be 65 DEG C of constant temperature, at this temperature detect sample tube in bacteria distribution it is fastest.Incubate
Educate the main resistance heating film for using 12V of module 02 to be heated, by TEMP
Device carries out temperature acquisition, and carries out accurate temperature controlling, temperature control precision to it by the CPU of equipment
Reachable ± 0.2 DEG C.
According to a kind of above-mentioned isothermal PCR detector, wherein, human-computer interaction module mainly includes key
Disk, battery capacity indication lamp and digital display screen, the connection of keyboard, battery capacity indication lamp and digital display screen
Computation analysis module (not shown) is controlled to CPU, primarily to finishing man-machine interaction
Wait work, wherein the function of keyboard for personnel operating area, such as adjustment the heat time, beat
Ultraviolet etc. is opened, the function of battery capacity indication lamp is the electricity for indicating device interior lithium ion battery,
The effect of the information such as heat time display measurement result is indicated outside the function of digital display screen.
According to a kind of above-mentioned detection method of isothermal PCR detector, including：
As depicted in figs. 1 and 2, power module 01, is respectively connecting to raising module 02, purple
Outside line irradiation module 03, light signal collection module 04, CPU control computation analysis module 05 and
Human-computer interaction module 06 is powered for it；
CPU controls computation analysis module 05, is respectively connecting to power module 01, raising module
02nd, ultraviolet irradiation module 03, light signal collection module 04 and human-computer interaction module 06 with
Control modules and calculating analysis detecting data；
Raising module 02, one or more constant temperature for being provided with 12V resistance heatings film to surround
Space is incubated, and one or more temperature sensors carry out temperature acquisition, it is one or more of
Temperature sensor is connected the temperature for collecting temperature sensor with CPU controls computation analysis module
Degrees of data send to CPU control computation analysis module with control raising module temperature (in figure not
Show)；
One or more detection modules, respectively in one or more constant-temperature incubation spaces, often
Individual detection module is provided with one or more groups of detection holes, and every group of detection hole includes multiple detection holes,
The testing sample that will be handled well is placed in centrifuge tube, and centrifuge tube is placed in detection hole and is detected
(not shown)；
Ultraviolet irradiation module 03, including multiple LED light sources 07, be separately positioned on one or
Below each detection hole 08 on multiple detection modules；
Light signal collection module 04, including multiple light signal acquisition devices 09, are separately positioned on
The side in each the detection hole 08 on one or more detection modules；
Optical process modules, including multiple incidence optical processors 10 and multiple excite optical processor
11, it is provided between each LED light source 07 and corresponding detection hole 08 at an incident light
Reason is respectively provided between device 10, and each light signal acquisition device 09 and corresponding detection hole 08
There is one to excite optical processor 11, result in formation of multigroup and multiple sense channels；And
Human-computer interaction module 06, mainly including keyboard, battery capacity indication lamp and digital display screen,
It is connected to CPU and controls computation analysis module 05 to realize man-machine interaction (not shown).
The above, is only the present invention preferably implementation method, not to technical side of the invention
Case does any formal limitation.It is every above example is done according to technical spirit of the invention
Any simple modification, form change and modification, each fall within protection scope of the present invention.
Claims (10)
1. a kind of isothermal PCR detector, it is characterised in that：The isothermal PCR detector bag
Include：Power module, raising module, one or more detection modules, ultraviolet irradiation module,
Optical process modules, light signal collection module, CPU control computation analysis module and man-machine interaction
Module, the power module be respectively connecting to the raising module, the ultraviolet irradiation module,
The light signal collection module, the CPU control computation analysis module and the man-machine interaction mould
Block, the CPU controls computation analysis module is respectively connecting to the power module, the incubation
Module, the ultraviolet irradiation module, the light signal collection module and the man-machine interaction mould
Block, one or more of detection modules are respectively positioned at the 12V resistance heatings of the raising module
In one or more constant-temperature incubation spaces that film is surrounded, the ultraviolet irradiation module and the light
Signal acquisition module is located at below one or more of detection modules and side respectively, described
Optical process modules are located at the ultraviolet irradiation module and one or more of detection modules
Between or the light signal collection module and one or more of detection modules between.
2. a kind of isothermal PCR detector according to claim 1, it is characterised in that：
Each detection module in one or more of detection modules is provided with one or more groups of detections
Hole, every group of detection hole includes multiple detection holes, wherein, one or more of detection modules are
One or more aluminium blocks.
3. a kind of isothermal PCR detector according to claim 1, it is characterised in that：
The ultraviolet irradiation module includes multiple LED light sources, and the multiple LED light source is respectively provided with
Below each detection hole on one or more detection modules.
4. a kind of isothermal PCR detector according to claim 1, it is characterised in that：
The light signal collection module includes multiple light signal acquisition devices, the multiple light signal collection
Device is separately positioned on the side in each the detection hole on one or more detection modules.
5. a kind of isothermal PCR detector according to claim 1, it is characterised in that：
The optical process modules include that multiple incidence optical processors and multiple excite optical processor, each
An incident optical processor, and each light are provided between LED light source and corresponding detection hole
One is provided between signal pickup assembly and corresponding detection hole excite optical processor.
6. a kind of isothermal PCR detector according to claim 1, it is characterised in that：
The power module includes 24V external power adapters and chargeable lithium cell, outside the 24V
Power supply adaptor and the chargeable lithium cell is connect to be connected with mains 220V power supplys respectively,
And the 24V external power adapters are depressured part and connect behind power filter part by 12V
CPU controls are respectively connecting to the 12V resistance heatings film of raising module or by 5V step-downs part
Computation analysis module processed, multiple LED light sources of ultraviolet irradiation module, light signal collection module
Multiple light signal acquisition devices and human-computer interaction module；And the chargeable lithium cell passes through
12V step-downs are partially attached to the 12V resistance heatings film of raising module or are depressured part point by 5V
Be not connected to CPU control computation analysis module, multiple LED light sources of ultraviolet irradiation module,
Multiple light signal acquisition devices and human-computer interaction module of light signal collection module.
7. a kind of isothermal PCR detector according to claim 6, it is characterised in that：
The chargeable lithium cell is provided with management of charging and discharging module, the management of charging and discharging module with
CPU control computation analysis module connections.
8. a kind of isothermal PCR detector according to claim 1, it is characterised in that：
The raising module is provided with one or more temperature sensors and carries out temperature acquisition, one
Or multiple temperature sensors are connected with CPU controls computation analysis module.
9. a kind of isothermal PCR detector according to claim 1, it is characterised in that：
The human-computer interaction module mainly includes keyboard, battery capacity indication lamp and digital display screen, the key
Disk, the battery capacity indication lamp and the digital display screen are connected to CPU control computation analysis modules.
10. a kind of detection method of isothermal PCR detector, it is characterised in that：Methods described bag
Include：
Power module, is respectively connecting to raising module, ultraviolet irradiation module, light signal collection
Module, CPU control computation analysis module and human-computer interaction module to be powered for it,
CPU controls computation analysis module, is respectively connecting to power module, raising module, ultraviolet
Line irradiation module, light signal collection module and human-computer interaction module are controlling modules and calculating
Analysis detecting data；
Raising module, one or more constant-temperature incubations for being provided with 12V resistance heatings film to surround
Space, and one or more temperature sensors carry out temperature acquisition, one or more of temperature
Sensor is connected the temperature data for collecting temperature sensor with CPU controls computation analysis module
Send to CPU and control computation analysis module to control the temperature of raising module；
One or more detection modules, respectively in one or more constant-temperature incubation spaces, often
Individual detection module is provided with one or more groups of detection holes, and every group of detection hole includes multiple detection holes,
The testing sample that will be handled well is placed in centrifuge tube, and centrifuge tube is placed in detection hole and is detected；
Ultraviolet irradiation module, including multiple LED light sources, are separately positioned on one or more inspections
Below each the detection hole surveyed in module；
Light signal collection module, including multiple light signal acquisition devices, be separately positioned on one or
The side in each the detection hole on multiple detection modules；
Optical process modules, including multiple incidence optical processors and multiple excite optical processor, often
An incident optical processor, and each are provided between individual LED light source and corresponding detection hole
One is provided between light signal acquisition device and corresponding detection hole excite optical processor, this
Sample forms multigroup and multiple sense channels；And
Human-computer interaction module, mainly including keyboard, battery capacity indication lamp and digital display screen, connects
It is connected to CPU and controls computation analysis module to realize man-machine interaction.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510874401.4A CN106834110A (en) | 2015-12-04 | 2015-12-04 | A kind of isothermal PCR detector and its detection method |
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| CN201510874401.4A CN106834110A (en) | 2015-12-04 | 2015-12-04 | A kind of isothermal PCR detector and its detection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117286017A (en) * | 2023-11-21 | 2023-12-26 | 瓜藤生物科技(深圳)有限公司 | Multichannel nucleic acid amplification analysis equipment |
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Address after: Room 201, No. 1 Xingchang Road, Changping District Science and Technology Park, Beijing 102200 Applicant after: Medi Weidun (Beijing) Technology Development Co., Ltd. Address before: Room 201, No. 1 Xingchang Road, Changping District, Beijing 102200 Applicant before: BEIJING BIOCHEM HUAYE TECHNOLOGY DEVELOPMENT CO., LTD. |
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Application publication date: 20170613 |