CN106834084A - A kind of user oriented DNA collections assay system - Google Patents
A kind of user oriented DNA collections assay system Download PDFInfo
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- CN106834084A CN106834084A CN201710064531.0A CN201710064531A CN106834084A CN 106834084 A CN106834084 A CN 106834084A CN 201710064531 A CN201710064531 A CN 201710064531A CN 106834084 A CN106834084 A CN 106834084A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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Abstract
The invention particularly discloses a kind of user oriented DNA collections assay system, including biological label Acquisition Instrument, biological label filtrator, DNA sample chemical examination instrument and DNA extraction modules.The present invention associates personal microcosmic gene information with least one apparent personal information, such as DNA information and fingerprint, DNA information and acoustic information, or DNA information is associated with iris information.The highly integrated database of the present invention, can be by data based on the user for having carried out DNA collections, it is followed successively by whether the user according to judgement below has carried out DNA collections, share built file data, avoid DNA from repeating chemically examine, file, cause the repeated work of lab assistant and bring the waste of the related resources such as storage, experiment.
Description
Technical field
The present invention relates to physical characteristics collecting chemical examination field, more particularly to DNA collection chemical examinations, in particular it relates to a kind of
User oriented DNA gathers assay system.
Background technology
Gene (gene) is the DNA fragmentation with hereditary effect.Gene supports essential structure and the performance of life.
Store the race of life, blood group, breed, grow, the full detail of the process such as apoptosis.Interdepending for E＆H, drills
Unravel silk the important physiology courses such as procreation, cell division and the protein synthesis of life.Organism it is sick, long, decline, it is old, wait indefinitely
All life phenomenon is all relevant with gene.It is also the internal factor for determining life and health.Therefore, gene has double attribute：
Materiality (existing way) and informedness (fundamental nature).
Therefore, DNA chemical examinations are widely used in the fields such as personal information record, medical treatment, Criminal Investigation.In the prior art,
When certain department needs collection DNA information, all right chemical examination for being carried out to user again, file.Many users may be at it in fact
His place had carried out DNA information chemical examination collection, and simply its chemical examination collection result is not shared.Cause the DNA for having filed
Can repeat to chemically examine, file, cause the repeated work of lab assistant and bring the waste of the related resources such as storage, experiment.
The content of the invention
For the deficiency in prior art, it is an object of the invention to provide a kind of user oriented DNA collections chemical examination system
System.
In order to solve the above-mentioned technical problem, the present invention is achieved by the following technical programs：
A kind of user oriented DNA collections assay system, including biological label Acquisition Instrument, biological label filtrator, DNA samples
This chemical examination instrument and DNA extraction modules, the biological label Acquisition Instrument are used to obtain the biological label group of each user to be assayed,
Wherein, biological label group include the DNA sample of a corresponding user to be assayed and in addition to the DNA sample at least
A kind of biological label；The biological label filtrator is used to filter out to need at least one target organism mark for carrying out DNA chemical examinations
Knowledge group；The DNA sample chemical examination instrument is used to carry out DNA chemical examinations at least one target organism mark group, and records right
The DNA sample result of laboratory test answered, the DNA extraction modules are used to extract user's genomic DNA.
Preferably, also including ID maker, the personal information account for setting up each user to be assayed is described
ID maker generates personal unique information-account based on the fingerprint or acoustic information of user；The personal information
Account is also including personal essential information such as name, sex, date of birth, ID card No., address and contact method.
Relative to prior art, beneficial effects of the present invention：
1st, personal microcosmic gene information associate by the present invention with least one apparent personal information, such as DNA information with
Fingerprint, DNA information and acoustic information, or DNA information are associated with iris information.
2nd, the characteristics of biological label filtrator is according to the uniqueness and consistency that set biological label, by repeated acquisition
Biological label group judgement, filtering out needs the target organism mark group for carrying out DNA chemical examinations, is further advanced by DNA sample
Chemical examination instrument is chemically examined the DNA sample for needing to carry out DNA chemical examinations, and is filed for result of laboratory test, drastically increases system
Efficiency, saved cost.
3rd, highly integrated database, data based on the user for having carried out DNA collections can be followed successively by foundation and sentenced
Whether the user in face of having no progeny has carried out DNA collections, shares built file data, it is to avoid DNA repeats chemically examine, file, and causes laboratory
The repeated work of personnel simultaneously brings the waste of the related resources such as storage, experiment.
Brief description of the drawings
Accompanying drawing herein is merged in specification and constitutes the part of this specification, shows and meets implementation of the invention
Example, and be used to explain principle of the invention together with specification.
Fig. 1 is structural representation of the invention.
Specific embodiment
The invention will be further described with the following Examples.
Fig. 1 is the structural representation of one embodiment of the invention, as shown in Figure 1.
A kind of user oriented DNA collections assay system, including biological label Acquisition Instrument, biological label filtrator, DNA samples
This chemical examination instrument and DNA extraction modules, the biological label Acquisition Instrument are used to obtain the biological label group of each user to be assayed,
Wherein, biological label group include the DNA sample of a corresponding user to be assayed and in addition to the DNA sample at least
A kind of biological label；The biological label filtrator is used to filter out to need at least one target organism mark for carrying out DNA chemical examinations
Knowledge group；The DNA sample chemical examination instrument is used to carry out DNA chemical examinations at least one target organism mark group, and records right
The DNA sample result of laboratory test answered, the DNA extraction modules are used to extract user's genomic DNA.
Preferably, also including ID maker, the personal information account for setting up each user to be assayed is described
ID maker generates personal unique information-account based on the fingerprint or acoustic information of user.
Preferably, also including memory module, the memory module also includes DNA collection analysis datas storehouse, the database
Storage has carried out the user profile of DNA collection chemical examinations, and judges whether user below has carried out DNA collections based on this
Chemical examination.
Preferably, the biological label Acquisition Instrument includes DNA sample Acquisition Instrument and at least one biological label collection terminal, institute
At least one biological label collection terminal is stated for one or more in fingerprint collecting end, sound collection end, iris capturing end.
Preferably, the biological label filtrator, including at least one biological label duplicate checking device, the biological label are looked into
Treasure, for according to the corresponding biological label of all biological label groups for having collected, from most freshly harvested biological label group
The biological label group of non-repeated acquisition is filtered out, as the target organism mark group for needing to carry out DNA chemical examinations；Wherein, if newest
There are two biological label groups with identical biological label in the biological label group of collection, then randomly select one and judge whether
It is the biological label group of repeated acquisition.
The present invention associates personal microcosmic gene information with least one apparent personal information, such as DNA information and refers to
Line, DNA information and acoustic information, or DNA information are associated with iris information.Biological label filtrator is according to setting biological label
Uniqueness and consistency the characteristics of, by the judgement of the biological label group to repeated acquisition, filtering out needs to carry out DNA chemical examinations
Target organism mark group, be further advanced by DNA sample chemical examination instrument the DNA sample for needing to carry out DNA chemical examinations is chemically examined,
And filed for result of laboratory test, the efficiency of system is drastically increased, save cost.
As the presently preferred embodiments, the DNA extraction equipments that DNA extraction modules are used below be will be described in detail.
A kind of rapid extraction genomic DNA kit, including DNA Extraction buffers, washing lotion, eluent, storage liquid, centrifugation
Pipe, adsorption column and syringe, the DNA Extraction buffers main component include that cracking material, surfactant, chelating agent ease up
Electuary；The washing lotion includes washing lotion A and washing lotion B, and the eluent includes eluent I and eluent II；The centrifuge tube mouth of pipe
Place is provided with seal gasket；The built-in pellosil of adsorption column, built-in 2 filter layers in front end in the syringe.
Preferably, the DNA Extraction buffers composition be 5M guanidinium isothiocyanates, 10mM 2 mercapto ethanols, 50mM Tris,
20mM EDTA, 21.3mM Triton X-100s, pH 6.8；
The washing lotion A compositions are 4M guanidine thiocyanates, 50mMTris, pH 6.8；
The washing lotion B component is 10mM Tris, 100mMKCl, pH 7.4；
The composition of the eluent I is 10mM Tris-HCl, pH 7.8；1mM EDTA, pH 7.8；
The eluent II is 75% alcohol, pH 8.0；
The storage liquid composition is 100mM Tris-HCl, 10mM EDTA, 20mM ethanol；
The pellosil of the adsorption column is special silicon matrix sorbing material, can repel RNA and egg with specific adsorption DNA
White matter；The filter layer is the sponge filter layer of large aperture and the miillpore filter layer of extra small bore diameter.
Preferably, the filter layer sponge is the material of acid-fast alkali-proof；The filter layer miillpore filter is acid-fast alkali-proof
Ceramic membrane, aperture is 0.8nm~1 μm.
It is described to treat that DNA chemical examination collecting samples derive from human whole blood, Throat washing or fetus amniocyte.
Creatively be used in combination for guanidinium isothiocyanate and 2 mercapto ethanol by DNA Extraction buffers of the invention, carries significantly
Cell lysis efficiency high and cleavage rate, so as to improve the yield and purity of genomic DNA, this is gathered for follow-up DNA information
There are important quality assurance, high-quality genomic DNA, it is ensured that discrimination is higher.
Finally it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than the present invention is protected
The limitation of scope is protected, although being explained to the present invention with reference to preferred embodiment, one of ordinary skill in the art should
Work as understanding, technical scheme can be modified or equivalent, without deviating from the reality of technical solution of the present invention
Matter and scope.
Claims (4)
1. a kind of user oriented DNA gathers assay system, it is characterised in that including biological label Acquisition Instrument, biological label mistake
Filter instrument, DNA sample chemical examination instrument and DNA extraction modules, the biological label Acquisition Instrument are used to obtain each user's to be assayed
Biological label group, wherein, a biological label group includes the DNA sample of a corresponding user to be assayed and except the DNA samples
At least one biological label outside this；The biological label filtrator is used to filter out to need carry out DNA chemical examinations at least one
Individual target organism mark group；The DNA sample chemical examination instrument is used to carry out DNAization at least one target organism mark group
Test, and record corresponding DNA sample result of laboratory test, the DNA extraction modules are used to extract user's genomic DNA.
2. user oriented DNA gathers assay system according to claim 1, it is characterised in that also generated including ID
Device, the personal information account for setting up each user to be assayed, the ID maker is with the fingerprint or sound of user
Personal unique information-account is generated based on message breath.
3. user oriented DNA gathers assay system according to claim 2, it is characterised in that also including memory module, institute
Stating memory module also includes DNA collection analysis datas storehouse, and the database purchase has carried out user's letter of DNA collection chemical examinations
Breath, and judge whether user below has carried out DNA collection chemical examinations based on this.
4. user oriented DNA gathers assay system according to claim 3, it is characterised in that the biological label collection
Instrument includes DNA sample Acquisition Instrument and at least one biological label collection terminal, and at least one biological label collection terminal is fingerprint
One or more in collection terminal, sound collection end, iris capturing end.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710064531.0A CN106834084A (en) | 2017-02-04 | 2017-02-04 | A kind of user oriented DNA collections assay system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710064531.0A CN106834084A (en) | 2017-02-04 | 2017-02-04 | A kind of user oriented DNA collections assay system |
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| CN106834084A true CN106834084A (en) | 2017-06-13 |
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| CN201710064531.0A Pending CN106834084A (en) | 2017-02-04 | 2017-02-04 | A kind of user oriented DNA collections assay system |
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Citations (7)
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|---|---|---|---|---|
| CN101500694A (en) * | 2006-05-09 | 2009-08-05 | 先进液体逻辑公司 | Droplet manipulation systems |
| CN103314383A (en) * | 2010-11-01 | 2013-09-18 | 皇家飞利浦电子股份有限公司 | In vitro diagnostic testing including automated brokering of royalty payments for proprietary tests |
| CN103911366A (en) * | 2014-03-19 | 2014-07-09 | 华南理工大学 | Genome DNA extraction method and its kit |
| CN104573438A (en) * | 2013-10-29 | 2015-04-29 | 江苏华御信息技术有限公司 | System and method for managing file based on file biological label |
| CN204474677U (en) * | 2015-02-06 | 2015-07-15 | 大连医科大学附属第二医院 | A kind ofly collect nucleic acid extraction, increase, be detected as the micro-fluidic chip of one |
| CN105349401A (en) * | 2015-10-14 | 2016-02-24 | 安徽易康达光电科技有限公司 | Multifunctional integrated microfluidic nucleic acid analysis chip and preparation and analysis method thereof |
| CN205188287U (en) * | 2015-06-29 | 2016-04-27 | 方正国际软件(北京)有限公司 | DNA gathers chemical examination system |
-
2017
- 2017-02-04 CN CN201710064531.0A patent/CN106834084A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101500694A (en) * | 2006-05-09 | 2009-08-05 | 先进液体逻辑公司 | Droplet manipulation systems |
| CN103314383A (en) * | 2010-11-01 | 2013-09-18 | 皇家飞利浦电子股份有限公司 | In vitro diagnostic testing including automated brokering of royalty payments for proprietary tests |
| CN104573438A (en) * | 2013-10-29 | 2015-04-29 | 江苏华御信息技术有限公司 | System and method for managing file based on file biological label |
| CN103911366A (en) * | 2014-03-19 | 2014-07-09 | 华南理工大学 | Genome DNA extraction method and its kit |
| CN204474677U (en) * | 2015-02-06 | 2015-07-15 | 大连医科大学附属第二医院 | A kind ofly collect nucleic acid extraction, increase, be detected as the micro-fluidic chip of one |
| CN205188287U (en) * | 2015-06-29 | 2016-04-27 | 方正国际软件(北京)有限公司 | DNA gathers chemical examination system |
| CN105349401A (en) * | 2015-10-14 | 2016-02-24 | 安徽易康达光电科技有限公司 | Multifunctional integrated microfluidic nucleic acid analysis chip and preparation and analysis method thereof |
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Effective date of registration: 20170920 Address after: Xinhua Street, Huadu District, Guangdong city of Guangzhou province 510000 Tian Mei Cun Tian Mei Xin Cun from No. 87 Applicant after: Guangzhou Rui LAN embroidery Trading Co., Ltd. Address before: 200000, 556, 5, 2265 Willow Road, Shanghai, Putuo District Applicant before: Shanghai phase resistant Intelligent Technology Co., Ltd. |
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Application publication date: 20170613 |
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