CN106754357A - Wireless program-controlled illumination system and application for light science of heredity cell high pass quantity research - Google Patents
Wireless program-controlled illumination system and application for light science of heredity cell high pass quantity research Download PDFInfo
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- CN106754357A CN106754357A CN201611095124.8A CN201611095124A CN106754357A CN 106754357 A CN106754357 A CN 106754357A CN 201611095124 A CN201611095124 A CN 201611095124A CN 106754357 A CN106754357 A CN 106754357A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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Abstract
The invention discloses a kind of wireless program-controlled illumination system for light science of heredity cell high pass quantity research, the system includes host computer and control software, wireless communication module, slave computer central processing unit, slave computer register die group, slave computer light emitting diode group and constant voltage dc source.Device of the invention studies the research of Drug molecule screening especially related to signal path for the light science of heredity signal path of external scale evaluation cell, solve and lack high flux, many intensity, long-time, the plant issue of the stimulated in vitro of multifactor illumination in presently relevant research, effectively increase the accuracy and conventional efficient of light stimulus system in light genetics research.In addition, the condition of the cultured cells photoactivation obtained based on the system, such as intensity of illumination, photoactivation time are also had wide practical use for the light genetics research of cell in future.
Description
Technical field
It is more particularly to a kind of to be used for light science of heredity cell high pass the invention belongs to integrated electronic circuit and light genetic arts
The wireless program-controlled illumination system of quantity research and application.
Background technology
Light genetics technology is that for developing rapidly in recent years incorporates the complete of optics, genetic engineering and electronic engineering
The biotechnology of new multi-crossed disciplines.Using photogene technical controlling cellular activity, with not damaged, non-intruding, time-space resolution
Rate is high, can quantify repeat, using the advantage such as simple, got in research fields such as Neuscience, cell biology, signal paths
It is widely applied to get over.
So far, local excitation neuron (such as patent CN201510654472.3) is pinpointed by fiber-coupled diode
Or using Minitype LED array multiple spot light stimulus (such as Kwon K, Li W.Integrated multi-LED array with
three-dimensional polymer waveguide for optogenetics[C]//Micro Electro
Mechanical Systems(MEMS),2013IEEE 26th International Conference on.IEEE,2013:
1017-1020.), light science of heredity obtains very big achievement in neuroscience field.
But in more emerging signal path research field, light stimulus system also very it is simple and crude (such as Bugaj L J,
Choksi A T,Mesuda C K,et al.Optogenetic protein clustering and signaling
activation in mammalian cells[J].Nature methods,2013,10(3):249-252.)：1. lamp bead number
Amount is few, it is impossible to carry out high-throughout signal path molecule screening；2. can only be with single brightness irradiated stimulator cell, it is impossible to from software
On brightness is fine-tuned to probe into the relation that intensity of illumination and signal path activate degree；3. physical connection and computer expert are passed through
Letter, is not easy under incubator environment irradiate culture studies for a long time to cell.4. the same time can only with a kind of light enter assassinate
Swash.But signal path study, especially the Drug molecule screening related to signal path need high flux, many intensity, it is long when
Between and/or multifactor incentive condition.
The content of the invention
In view of above-mentioned shortcoming of the prior art, the invention provides a kind of for the wireless of light science of heredity high pass quantity research
Program control illumination system, the system can improve light genetics technology conventional efficient, reduce experimental cost, and the system can apply to
Carry out the control of light science of heredity high flux in the cell of in vitro culture.
A kind of wireless program-controlled illumination system for light science of heredity high pass quantity research, including：Host computer PC, bluetooth channel radio
News module, slave computer and constant voltage dc source, described slave computer include：Positioned at the centre of IC printed board one side
Reason unit, register die group, communication interface and power interface；Positioned at the light emitting diode of IC printed board another side
Group；And light emitting diode distribution is corresponding with each hole site of orifice plate used by biological cell assays；
Control instruction is delivered to centre by the control software in described host computer PC by blue tooth wireless communication module
Reason unit；
Described CPU receives the control instruction that blue tooth wireless communication slave sends, and by control instruction and when
Clock signal input register die group；
The serial input of control instruction is converted to parallel output by described register die group, and controls light emitting diode
Light-emitting mode, individually control the luminous of each Light-Emitting Diode；
Described light emitting diode group implements light stimulus in light genetics research to cell；
Described constant voltage dc source is used for the component offer work constant voltage dc source in slave computer.
Control software in the host computer PC is used to set light emitting diode (LED) bright light duration, flashing mode, light
Brightness and photochromic.
Described blue tooth wireless communication module includes：Blue tooth wireless communication main frame and blue tooth wireless communication slave, bluetooth without
Line host computer communication is connected by USB interface with host computer, and blue tooth wireless communication slave is assisted with blue tooth wireless communication main frame with bluetooth
View pairing；The blue tooth wireless communication main frame is instructed for emission control, and the blue tooth wireless communication slave is used to receive control
Instruction.
Preferably, described CPU, register die group and light emitting diode bond pads are integrated at one piece
On circuit board.
Preferably, described register die group includes processor register die group and light emitting diode register die
Group, processor register die group is connected with light emitting diode register die group, and control signal is converted into control luminous two
The level signal of the specific break-make of pole pipe.
Preferably, described light emitting diode group includes 96 light emitting diodes, into 8 × 12 rectangular arrangeds, each luminous two
Centre distance is 9mm between pole pipe, corresponding with each hole site of the porocyte culture plates of standard used by biological cell assays 96.
Preferably, described LED operation frequency is 40~110Hz, with PWM mode adjustment work brightness.Due to
The biological cell of in vitro culture is the insensitive body cell of frequency rather than the neuronal cell sensitive to light frequency, therefore works as illumination
Frequency more than after 40Hz, to impact cell ignore by illumination frequency.Further preferred illumination frequency is 50Hz, improves control essence
Degree.
Preferably, described light emitting diode outgoing optical wavelength includes 470nm blue lights, and 520nm green glows, 620nm feux rouges enters
Preferably, light emitting diode outgoing optical wavelength is 470nm to one step.
Light genetics research relies on light-dependent control element (photosensitive protein) and is transferred to target cell, and the activation of different light-dependent control elements needs
The coloured light of different wave length stimulates.Optimal wavelength is the blue light of 470nm, can effective stimulus cryptochrome class ligth activated element
CRY2/CIBN becomes to closing：After cryptochrome class photosensitive protein CRY2 absorbs 470nm blue lights, conformation changes, so that and part
PROTEIN C IBN is combined；And after blue light illumination is lost, cryptochrome class photosensitive protein CRY2 is in interior and ligandin for a period of time
CIBN is separated, and realizes the light-operated reversible of light heredity light-dependent control element.Therefore wavelength is the blue light stimulus of 470nm by preferably
It is applied in cell-signaling pathways research.
The physical size of the IC printed board is engaged with the porocyte culture plates of standard 96.
Described slave computer is connected by data flat cable with the blue tooth wireless communication slave.
Described DC power supply is connected by power interface with the slave computer.
The course of work of wireless program-controlled illumination system：Control software in host computer sends control instruction to bluetooth channel radio
Control instruction is transferred to news main frame, blue tooth wireless communication main frame blue tooth wireless communication slave, bluetooth in the way of wireless blue tooth
Control instruction is delivered to the CPU by wireless telecommunications slave by communication interface, and CPU refers to control
Order and clock signal input register die group, register die group control the light-emitting mode of light emitting diode, and control its right
Cell in light genetics research implements light stimulus, completes whole illumination.
Wireless program-controlled illumination system of the invention is used to carry out the control of light science of heredity high flux in the cell of in vitro culture, real
Show the wireless multithreading optics regulation and control under the conditions of cells in vitro i.e. incubator, and setting is preferably used for CIBN/CRY2 systems
The incentive condition of activation.
In the present invention, using the light stimulus of single-chip microcomputer and integrated circuit technique exploitation light genetics research field cell experiment
System, improves the accuracy and reliability of system, compared with the existing light genetics technology for being applied to signal path research, this
Invention has following beneficial technique effect：
(1) instrument utilization rate is high, and 96 light emitting diodes and 96 porocyte culture plates that illumination system has coordinate, each
LED lamp bead can individually be controlled by register die, thus single experiment can carry out repeatedly it is non-interfering quantitative
Analysis；
(2) light stimulus regulation and control are accurate, and the light intensity of light emitting diode can be by software adjustment linear change, the sudden strain of a muscle of light emitting diode
Bright frequency stabilization, in the absence of error；
(3) system can both be irradiated stimulation in normal culture environment to cell, it is also possible to by controlled in wireless
Realize the irradiation culture prolonged to cell in incubator environment；
(4) various monochrometric stimulus, can import various light-operated albumen, realize the research to signal path synergy；
(5) apparatus structure is succinct, easy to use, is quickly adjusted beneficial to according to experiment condition.
Brief description of the drawings
Fig. 1 is overall workflow frame diagram of the present invention；
Fig. 2 is the next machine face (light emitting diode face) structure chart in the embodiment of the present invention；
Fig. 3 is slave computer reverse side (component face) structure chart in the embodiment of the present invention；
Fig. 4 is the illumination operating diagram to 96 orifice plates in the embodiment of the present invention.
Specific embodiment
In order to more specifically describe the present invention, below in conjunction with the accompanying drawings and specific embodiment is to technical scheme
It is described in detail.
As shown in figure 1, wireless program-controlled illumination system of the present invention for light science of heredity high pass quantity research, including：Host computer
1st, Bluetooth wireless communication module 2, slave computer central processing unit 3, slave computer register die group 4 and slave computer LED groups 5.
Host computer 1 is to operate in the interactive program on PC, is the operation that user's control adjusts each LED light stimulus modality
Interface, the brightness (PWM duty cycle) of photic stimuli, bright light duration, flashing mode and photochromic four parameters are adjusted.Bluetooth
Wireless communication module 2 includes Bluetooth wireless communication main frame and Bluetooth wireless communication slave, and main frame turns USB interface and realizes by serial ports
With the communication of host computer 1, slave is then connected with slave computer data-interface 7, and after main frame is matched with slave, main frame is by host computer
1 control instruction packet is sent to slave with Bluetooth signal, is entered and is sent to slave computer central processing unit by slave computer data-interface 7
3.Slave computer central processing unit 3 is by control instruction and clock signal transmission to slave computer register die group 4.Slave computer register
Chipset 4 realizes that the break-make of slave computer LED groups 5 is controlled by controlling the negative electrode level height of slave computer LED groups 5, and then controls
The brightness of slave computer LED groups 5, bright light duration, flashing mode with it is photochromic.
As shown in Figures 2 and 3, the present invention is for the bottom in the wireless program-controlled illumination system of light science of heredity high pass quantity research
Machine includes：Slave computer LED groups 5, slave computer IC printed board 6, slave computer central processing unit 3, the deposit of slave computer processor
Device chipset 41, slave computer LED register dies group 42, slave computer data-interface 7, slave computer power interface 8.Wherein, it is the next
Machine data-interface 7 is connected with slave computer central processing unit 3, by the incoming slave computer central processing unit 3 of PC control signal；It is the next
Machine central processing unit 3 is connected with slave computer processor register die group 41, the I/O for extending slave computer central processing unit 3
Port, transmits control signal；Slave computer processor register die group 41 is connected with slave computer LED register dies group 42,
Control signal is converted into the level signal of the control specific break-makes of LED；Slave computer LED groups 5 include 96 LED, in 8 × 12 rows
Row, each adjacent LED distance is 9mm matching distance between each adjacent holes of 96 orifice plates；External dc power passes through the next electromechanical source
Interface 8 provides power supply for above-mentioned all slave computer components, and with the effect of preferable filtering voltage regulation.
The program of host computer 1 is write using C#；Bluetooth wireless communication module is using HC-06 and USB-TTL modules；Slave computer
Data-interface 7 is using row's pin socket；Slave computer central processing unit 3 uses STC15W408AS；Slave computer processor register die
Group 41 uses 74HC595；It is core circuit that slave computer LED register dies group 42 uses PIC10F200；Slave computer LED groups 5 are adopted
Use common anode three-color LED；Slave computer power interface 8 uses DC005 power outlets and filter regulator circuit；Power supply using AC220V~
DC5V 10A voltage stabilizing adapters.The working frequency of LED fixes 50Hz；Brightness, brightness degree 100 are adjusted with PWM mode；Feux rouges work
Make wavelength for 630~640nm；Green glow operation wavelength is 515-525nm；Blue light operation wavelength is 465-475nm；Cell trench bottom
Three kinds of light highlight fluxs are 90lx.
Illumination work structuring such as Fig. 4 institute of the present invention for the wireless program-controlled illumination system of light science of heredity high pass quantity research
Show, wherein, special 96 orifice plate 12 is different from common transparent 96 orifice plate in market, but the orifice plate of black non transparent 96, to avoid one
The LED groups 5 of individual cell cultivation groove 11 are impacted to another culture tank.96 orifice plate plate lids 10 be also it is special, it is normal with market
See that plate lid identical is transparent capping, fit 96 orifice plates, but many black non transparent draw-in grooves 9 directly over each cell cultivation groove,
To support light control system slave computer IC printed board 6 and isolate each LED light source 5.Particularly, to avoid transparency cover 10
In diffusing reflection, the base portion of black non transparent draw-in groove 9 extends to the bottom of plate lid 10.
Workflow is：Cell culture fluid is injected into 96 orifice plate cell grooves 11；The lid of plate lid 10 is put on 96 orifice plates 12；Will
Downwards, each LED aligns and is buckled on plate lid 10 light control system slave computer LED with plate lid draw-in groove 9；By 96 orifice plates 12 and light control system
Slave computer is put into cell culture incubator together；Light control system host computer PC 1 sets illumination parameter and sends；Light control system slave computer is opened
Beginning light stimulation,；After the scheduled time arrives, host computer PC client 1 sends early warning, and slave computer stops illumination.
Method compared to reagent is filled using multiple culture dishes and gradation in traditional illumination research, the present invention has coordinated carefully
96 well culture plates commonly used in born of the same parents' experiment, can be used less reagent disposably to be filled by the volley of rifle fire, therefore use nothing of the invention
Thread control illumination system is greatly improved light genetics technology conventional efficient, while effectively reducing experimental cost, has reached high pass
Measure the purpose of optically screening.
Above-described specific embodiment has been described in detail to technical scheme and beneficial effect, Ying Li
Solution is to the foregoing is only presently most preferred embodiment of the invention, is not intended to limit the invention, all in principle model of the invention
Interior done any modification, supplement and equivalent etc. are enclosed, be should be included within the scope of the present invention.
Claims (10)
1. a kind of wireless program-controlled illumination system for light science of heredity high pass quantity research, including：Host computer PC, blue tooth wireless communication
Module, slave computer and constant voltage dc source, it is characterised in that：Described slave computer includes：Positioned at IC printed board one
The CPU in face, register die group, communication interface and power interface；Positioned at IC printed board another side
Light emitting diode group；And light emitting diode distribution is corresponding with each hole site of orifice plate used by biological cell assays；
It is single that control instruction is delivered to center treatment by the control software in described host computer PC by blue tooth wireless communication module
Unit；
Described CPU receives the control instruction that blue tooth wireless communication slave sends, and control instruction and clock are believed
Number input register chipset；
The serial input of control instruction is converted to parallel output by described register die group, and controls the hair of light emitting diode
Optical mode, individually controls the luminous of each Light-Emitting Diode；
Described light emitting diode group implements light stimulus in light genetics research to cell；
Described constant voltage dc source is used for the component offer work constant voltage dc source in slave computer.
2. wireless program-controlled illumination system according to claim 1, it is characterised in that：Described control instruction includes that control is sent out
Optical diode bright light duration, flashing mode, lamplight brightness and photochromic.
3. wireless program-controlled illumination system according to claim 1, it is characterised in that：Described blue tooth wireless communication module bag
Include：Blue tooth wireless communication main frame and blue tooth wireless communication slave, blue tooth wireless communication main frame pass through USB interface and host computer PC phase
Even, blue tooth wireless communication slave is matched with blue tooth wireless communication main frame with Bluetooth protocol.
4. wireless program-controlled illumination system according to claim 1, it is characterised in that：The thing of described IC printed board
Reason size is corresponding with the porocyte culture plates of standard 96.
5. wireless program-controlled illumination system according to claim 1, it is characterised in that：Described register die group includes place
Reason device register die group and light emitting diode register die group, processor register die group and light emitting diode register
Chipset is connected, and control signal is converted into the level signal of the control specific break-make of light emitting diode.
6. wireless program-controlled illumination system according to claim 5, it is characterised in that：Described light emitting diode group includes 96
Individual light emitting diode, into 8 × 12 rectangular arrangeds, centre distance is 9mm between each light emitting diode, is marked with used by biological cell assays
Accurate each hole site of 96 porocyte culture plates is corresponding.
7. the wireless program-controlled illumination system according to claim 1 or 6, it is characterised in that：Described light emitting diode group institute
The LED operation frequency for using is 40~100Hz, with PWM mode adjustment work brightness.
8. wireless program-controlled illumination system according to claim 7, it is characterised in that：Described light emitting diode outgoing light wave
It is long to include 470nm, 520nm and 620nm.
9. wireless program-controlled illumination system according to claim 1, it is characterised in that：Described CPU, deposit
Device chipset and light emitting diode bond pads are on one piece of surface-mounted integrated circuit.
10. light heredity is carried out in the cell that the wireless program-controlled illumination system according to claim 1~9 is any is cultivated in vitro
Learn the application in high flux control.
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| CN201611095124.8A CN106754357B (en) | 2016-12-01 | 2016-12-01 | Wireless program-controlled illumination system and application for light science of heredity cell high pass quantity research |
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| CN201611095124.8A CN106754357B (en) | 2016-12-01 | 2016-12-01 | Wireless program-controlled illumination system and application for light science of heredity cell high pass quantity research |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109735450A (en) * | 2019-03-11 | 2019-05-10 | 中国医学科学院生物医学工程研究所 | A kind of low energy light source shelf for cell incubator |
| CN111103272A (en) * | 2018-10-26 | 2020-05-05 | 山东大学 | Real-time screening and measuring system and method for cell specific photosensitive effect |
| CN112831418A (en) * | 2021-04-07 | 2021-05-25 | 广州市丹树生物科技有限责任公司 | In-vitro high-flux screening device for phototherapy and application thereof |
| WO2022126363A1 (en) * | 2020-12-15 | 2022-06-23 | 深圳先进技术研究院 | Cell phenotype control device |
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