CN106733886B - Full-automatic high-efficient western blot membrane washing system - Google Patents
Full-automatic high-efficient western blot membrane washing system Download PDFInfo
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- CN106733886B CN106733886B CN201611187775.XA CN201611187775A CN106733886B CN 106733886 B CN106733886 B CN 106733886B CN 201611187775 A CN201611187775 A CN 201611187775A CN 106733886 B CN106733886 B CN 106733886B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/10—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
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Abstract
The invention relates to a full-automatic high-efficiency western blot membrane washing system.A membrane washing device upper shell is in a hollow platform shape and is matched with a membrane washing device base, and a water pump device, an electric device, a rocker arm device and a transmission device are fixed on the membrane washing device base; the lifting liquid changing device is connected with the water pump device and is arranged close to the membrane washing box, the membrane washing box is positioned on the shaking table and can be taken and placed, the shaking table is driven by the rocker arm device, and the electric device drives the rocker arm device through the transmission device; the electric device and the water pump device are controlled by a control panel, and the control panel respectively controls the water pump device and the electric device. The invention has the advantages of simple operation, short time consumption, small reagent consumption, low labor cost, automation and high efficiency.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a full-automatic high-efficiency western blot membrane washing system used in a biomedical basic experiment.
Background
Western blot, western blotting, is a commonly used experimental method in molecular biology, biochemistry and immunogenetics. The basic principle is to stain the cell or biological tissue sample treated by gel electrophoresis through specific antibody, and obtain the information of the expression condition of specific protein in the analyzed cell or tissue through analyzing the stained position and stained depth. The Western blot steps roughly comprise the steps of sample preparation, protein content quantification, SDS-PAGE electrophoresis, membrane transfer (PVDF membrane or NC membrane), sealing, membrane washing, antibody incubation, chemiluminescence and the like, and the whole process is long in time consumption. Among them, membrane washing is an important step for the purpose of removing unbound blocking reagent or non-specifically bound antibody from the membrane. The whole Western Blot experiment process needs 2-3 times of membrane washing, and a single membrane washing needs 5min multiplied by 3 times or 10-15min multiplied by 2 times, so that the total time consumption is long.
The conventional membrane washing method is usually a manual operation of a table type shaking table combined with a timer, and an operator needs to perform liquid transfer and liquid adding for many times in a timed manner. Compared with the traditional method, the American Next advanced free roller full-automatic membrane washing system is available in the market, the system can automatically complete the steps of sealing → washing → adding primary antibody → washing → recycling primary antibody → washing → adding secondary antibody → recycling secondary antibody → washing, and the like, and can completely replace the traditional manual operation method, but the system still has the following defects: the western blot step is complete in coverage and slightly redundant, and particularly covers the step of automatically adding and recovering the antibody, so that the use efficiency of the antibody cannot be guaranteed; 2. the washing film box is large, and washing liquid is wasted; 3. the liquid washing (transferring) pipe is easy to contact with the PVDF membrane or the NC membrane, and the liquid discharge is incomplete; 4. the volume is large, and the occupied space is wide; 5. the price is high, and the market utilization rate is low.
Disclosure of Invention
The invention aims to provide a full-automatic high-efficiency western blot membrane washing system which is used for automatically washing membranes, automatically adding/transferring liquid and is simple and convenient to operate, aiming at the defects of inconvenient operation, long time consumption, high reagent consumption and high labor cost in the traditional method.
A full-automatic high-efficiency western blot membrane washing system comprises:
the membrane washing device comprises a membrane washing device upper shell, a membrane washing device lower shell and a membrane washing device, wherein the membrane washing device upper shell is in a hollow platform shape and is matched with a membrane washing device base, and a water pump device, an electric device, a rocker arm device and a transmission device are fixed on the membrane washing device base;
the lifting liquid changing device is connected with the water pump device and is placed close to the membrane washing box, the membrane washing box is positioned on the shaking table, the shaking table is driven by the rocker arm device, and the electric device drives the rocker arm device through the transmission device;
the electric device and the water pump device are controlled by a control panel, and the control panel respectively controls the water pump device and the electric device.
The upper part of the upper shell of the membrane washing device is provided with a liquid changing rod lifting hole and a rocker arm moving hole, the side wall of the upper shell of the membrane washing device is provided with a control panel mounting end, a waste liquid bottle connecting end and a washing liquid bottle connecting end, the control panel mounting end is fixedly provided with a control panel, and the control panel is connected with a touch screen.
Wash membrane device base upper portion and be equipped with biax motor installation base, first synchronizing wheel unable adjustment base, unipolar motor installation base, second synchronizing wheel unable adjustment base, first rocker guide rail, second rocker guide rail, third rocker guide rail and lead screw motor base, be fixed with first rocker guide rail, second rocker guide rail between biax motor installation base and the first synchronizing wheel unable adjustment base, biax motor installation base is two, fixes electric actuator's biax motor, first synchronizing wheel unable adjustment base is two, the synchronizing wheel of fixed transmission respectively, unable adjustment base fixes third rocker guide rail between unipolar motor installation base and the second synchronizing wheel, the fixed liftable of lead screw motor base trades the liquid device.
The liftable trade liquid device includes liftable trade liquid pole and trades liquid pole mount, trade the liquid pole mount and fix in the bottom of liftable trade liquid pole through trading liquid pole lead screw hole to lead screw motor drive through electric actuator, liftable trade the liquid pole and be 7 fonts, upper portion is located the upper portion of washing the diaphragm chamber, and is equipped with the second and annotates liquid mouth and second leakage fluid dram, and the bottom is equipped with first notes liquid mouth and first drainage fluid dram.
The membrane washing box is provided with a liquid changing bin and a membrane washing bin, the bottom of the membrane washing box is provided with a membrane washing box limiting groove, and a washing liquid circulation hole is formed in the membrane washing box.
Shaking table upper portion is equipped with the capsule of washing draw-in groove and the spacing post of capsule of washing, and the lower part is equipped with the fixed arm lock of open end rocking arm, first rocking arm connecting rod link and first stiff end rocking arm link, the capsule of washing draw-in groove is two, presss from both sides tight capsule of washing, the spacing post of capsule of washing cooperates with the capsule of washing spacing groove of capsule of washing, the fixed arm lock of open end rocking arm is two, blocks the open end rocking arm of rocking arm device, first rocking arm connecting rod link is connected with the rocking arm connecting rod of rocking arm device, first stiff end rocking arm link is connected with the stiff end rocking arm of rocking arm device.
The water pump device comprises a liquid suction pump and a liquid drainage pump, the liquid suction pump and the liquid drainage pump are respectively fixed on a water pump fixing frame, and the water pump fixing frame is fixed on a membrane washing device base.
The electric device comprises a double-shaft motor, a single-shaft motor, a lead screw motor with a guide rail and a lead screw motor, the double-shaft motor is fixed on a double-shaft motor mounting base, the single-shaft motor is fixed on the single-shaft motor mounting base, the lead screw motor with the guide rail is fixed on an open end rocker arm of a rocker arm device, and the lead screw motor is fixed on a lead screw motor base.
The rocking arm device includes open end rocking arm, stiff end rocking arm, over-and-under type rocking arm and rocking arm connecting rod, open end rocking arm is L shape, and the bottom is equipped with first synchronous wheel area fixed orifices and first guide rail sliding hole, first synchronous wheel area fixed orifices passes transmission's synchronous wheel area, first guide rail sliding hole overlaps respectively on the first rocking arm guide rail of washing membrane device base and second rocking arm guide rail, the stiff end rocking arm is the straight line form, and the bottom is equipped with second synchronous wheel area fixed orifices and second guide rail sliding hole, second guide rail slip Kong Taozai washes on the third rocking arm guide rail of membrane device base, second synchronous wheel area fixed orifices passes transmission's synchronous wheel area, over-and-under type rocking arm and rocking arm connecting rod are articulated, the rocking arm connecting rod passes through the rocking arm movable hole of the rocking arm of washing membrane device epitheca and fixes on the rocking bed, the over-and-under type rocking arm passes through electric actuator's area guide rail lead screw motor and fixes on open end rocking arm.
The transmission device comprises a synchronizing wheel, a synchronizing wheel belt, an open-end synchronizing wheel fixing shaft and a fixed-end synchronizing wheel fixing shaft, wherein the synchronizing wheel is respectively sleeved on the open-end synchronizing wheel fixing shaft and the fixed-end synchronizing wheel fixing shaft.
The upper shell of the membrane washing device is in a hollow platform shape and is matched with a base of the membrane washing device, and a water pump device, an electric device, a rocker arm device and a transmission device are fixed on the base of the membrane washing device; the lifting liquid changing device is connected with the water pump device and is arranged close to the membrane washing box, the membrane washing box is positioned on the shaking table, the shaking table is driven by the rocker arm device, and the electric device drives the rocker arm device through the transmission device; the electric device and the water pump device are controlled by a control panel, and the control panel respectively controls the water pump device and the electric device. The invention has the advantages of simple operation, short time consumption, small reagent consumption, low labor cost, automation and high efficiency.
Drawings
FIG. 1 is a perspective view of the present invention;
FIG. 2 is an exploded view of the present invention;
FIG. 3 is a top view of the upper housing of the membrane washing device of the present invention;
FIG. 4 is a front view of the upper housing of the membrane washing device of the present invention;
FIG. 5 is a top view of the capsule of the present invention;
FIG. 6 is a bottom view of the capsule of the present invention;
FIG. 7 isbase:Sub>A cross-sectional view of the invention A-A;
FIG. 8 is a right side view of the liftable liquid exchange device of the present invention;
FIG. 9 is a front view of the liftable liquid-changing device of the present invention;
FIG. 10 is a cross-sectional view of the invention B-B;
FIG. 11 is a front view of a rocking platform according to the invention;
FIG. 12 is a bottom view of a swing bed according to the present invention;
FIG. 13 is a front view of the open end rocker arm of the present invention;
FIG. 14 is a front view of a fixed end rocker arm of the present invention;
FIG. 15 is a right side view of the fixed end rocker arm of the present invention;
FIG. 16 is a front view of the lift rocker arm of the present invention;
FIG. 17 is a right side view of the lift rocker arm of the present invention;
FIG. 18 is a bottom plan view of the lift rocker arm of the present invention;
FIG. 19 is a front view of the rocker link of the present invention;
FIG. 20 is a right side elevational view of the rocker link of the present invention;
FIG. 21 is a control connection diagram of the present invention;
FIG. 22 is a comparison of different capsule washers according to the present invention;
FIG. 23 is a cross-sectional view of the present invention C-C;
in the figure: 1. a membrane washing device upper shell, 2, a membrane washing device base, 3, a lifting liquid changing device, 4, a membrane washing box, 5, a shaking table, 10, a control panel, 11, a control panel mounting end, 12, a liquid changing rod lifting hole, 13, a rocker arm movable hole, 14, a waste liquid bottle connecting end, 15, a liquid washing bottle connecting end, 21, a double-shaft motor mounting base, 22, a first synchronous wheel fixing base, 23, a single-shaft motor mounting base, 24, a second synchronous wheel fixing base, 25, a first rocker arm guide rail, 26, a second rocker arm guide rail, 27, a third rocker arm guide rail, 28, a screw rod motor base, 31, a lifting liquid changing rod, 32, a liquid changing rod fixing frame, 41, a liquid changing bin, 42, a membrane washing bin, 43, a washing liquid flowing hole, 44, a membrane washing box limiting groove, 51, a membrane washing box clamping groove, 52, an open end rocker arm fixing clamping arm, 53, a first rocker arm connecting end, 54, a first fixed end connecting end, 55, the device comprises a capsule washing box limiting column, 61, a liquid suction water pump, 62, a liquid discharge water pump, 63, a water pump fixing frame, 71, a double-shaft motor, 72, a single-shaft motor, 73, a lead screw motor with a guide rail, 74, a lead screw motor, 81, an open end rocker arm, 82, a fixed end rocker arm, 83, a lifting rocker arm, 84, a rocker arm connecting rod, 91, a synchronizing wheel, 92, a synchronizing wheel belt, 93, a synchronizing wheel fixing shaft with an open end, 94, a fixed end synchronizing wheel fixing shaft, 311, a first liquid injection port, 312, a first liquid discharge port, 313, a second liquid injection port, 314, a second liquid discharge port, 315, a liquid changing rod screw rod hole, 811, a first synchronizing wheel belt fixing hole, 812, a first guide rail sliding hole, 821, a second synchronizing wheel belt fixing hole, 822, a second guide rail sliding hole, 823, a second rocker arm connecting end, 831, a screw rod hole, 832, a motor guide rail hole, 833 and a second rocker arm connecting end.
Detailed Description
The invention is further described below with reference to the following figures and specific examples.
Example 1:
a full-automatic high-efficiency western blot membrane washing system comprises: the membrane washing device comprises a membrane washing device upper shell 1, wherein the membrane washing device upper shell 1 is in a hollow platform shape and is matched with a membrane washing device base 2, and a water pump device, an electric device, a rocker arm device and a transmission device are fixed on the membrane washing device base 2; the lifting liquid changing device 3 is connected with the water pump device and is arranged close to the membrane washing box 4, the membrane washing box 4 is positioned on the shaking table 5, the shaking table 5 is driven by the rocker arm device, and the electric device drives the rocker arm device through the transmission device; the electric device and the water pump device are controlled by a control board 10, and the control board 10 respectively controls the water pump device and the electric device.
The upper part of the upper shell 1 of the film washing device is provided with a liquid exchange rod lifting hole 12 and a rocker arm movable hole 13, the side wall of the upper shell is provided with a control panel mounting end 11, a waste liquid bottle connecting end 14 and a washing liquid bottle connecting end 15, the control panel mounting end 11 is used for fixing a control panel, and the control panel 10 is connected with a touch screen. The upper portion of the film washing device base 2 is provided with a double-shaft motor installation base 21, a first synchronous wheel fixing base 22, a single-shaft motor installation base 23, a second synchronous wheel fixing base 24, a first rocker guide rail 25, a second rocker guide rail 26, a third rocker guide rail 27 and a lead screw motor base 28, the double-shaft motor installation base 21 and the first synchronous wheel fixing base 22 are used for fixing the first rocker guide rail 25 and the second rocker guide rail 26 respectively, the number of the double-shaft motor installation base 21 is two, a double-shaft motor 71 for fixing an electric device is fixed, the number of the first synchronous wheel fixing base 22 is two, synchronous wheels 91 for fixing a transmission device respectively, the single-shaft motor installation base 23 and the second synchronous wheel fixing base 24 are used for fixing the third rocker guide rail 27, and the lead screw motor base 28 is used for fixing the lifting liquid changing device 3.
The liftable liquid replacing device 3 comprises a liftable liquid replacing rod 31 and a liquid replacing rod fixing frame 32, the liquid replacing rod fixing frame 32 is fixed at the bottom of the liftable liquid replacing rod 31 through a liquid replacing rod screw rod hole 315 and is driven by a screw rod motor 74 of an electric device, the liftable liquid replacing rod 31 is 7-shaped, the upper part of the liftable liquid replacing rod is positioned at the upper part of the membrane washing box 4 and is provided with a second liquid injection port 313 and a second liquid discharge port 314, and the bottom of the liftable liquid replacing rod fixing frame is provided with a first liquid injection port 311 and a first liquid discharge port 312. The membrane washing box 4 is provided with a liquid changing bin 41 and a membrane washing bin 42, the bottom of the membrane washing box is provided with a membrane washing box limiting groove 44, and the inside of the membrane washing box is provided with a washing liquid circulation hole 43. Shaking table 5 upper portion is equipped with washes diaphragm capsule draw-in groove 51 and washes the spacing post 55 of diaphragm capsule, the lower part is equipped with open end rocking arm fixed arm 52, first rocking arm connecting rod link 53 and first stiff end rocking arm link 54, it is two to wash diaphragm capsule draw-in groove 51, press from both sides tight diaphragm capsule 4, the spacing post 55 of diaphragm capsule is cooperated with the diaphragm capsule washing box spacing groove 44 of diaphragm capsule 4, open end rocking arm fixed arm 52 is two, block the open end rocking arm 81 of rocking arm device, first rocking arm connecting rod link 53 is connected with the rocking arm connecting rod 84 of rocking arm device, first stiff end rocking arm link 54 is connected with the stiff end rocking arm 82 of rocking arm device.
The water pump device comprises a liquid suction water pump 61 and a liquid discharge water pump 62, the liquid suction water pump 61 and the liquid discharge water pump 62 are respectively fixed on a water pump fixing frame 63, and the water pump fixing frame 63 is fixed on the membrane washing device base 2. The electric device comprises a double-shaft motor 71, a single-shaft motor 72, a lead screw motor 73 with a guide rail and a lead screw motor 74, wherein the double-shaft motor 71 is fixed on a double-shaft motor mounting base 21, the single-shaft motor 72 is fixed on a single-shaft motor mounting base 23, the lead screw motor 73 with the guide rail is fixed on a rocker arm 81 at the placing end of the rocker arm device, and the lead screw motor 74 is fixed on a lead screw motor base 28.
The rocker arm device comprises an open-end rocker arm 81, a fixed-end rocker arm 82, a lifting rocker arm 83 and a rocker arm connecting rod 84, wherein the open-end rocker arm 81 is L-shaped, the bottom of the open-end rocker arm is provided with a first synchronous belt fixing hole 811 and a first guide rail sliding hole 812, the first synchronous belt fixing hole 811 penetrates through a synchronous belt 92 of the transmission device, the first guide rail sliding hole 812 is respectively sleeved on a first rocker arm guide rail 25 and a second rocker arm guide rail 26 of the film washing device base 2, the fixed-end rocker arm 82 is linear, the bottom of the fixed-end rocker arm is provided with a second synchronous belt fixing hole 821 and a second guide rail sliding hole 822, the second guide rail sliding hole 822 is sleeved on a third rocker arm guide rail 27 of the film washing device base 2, the second synchronous belt fixing hole 821 penetrates through a synchronous belt 92 of the transmission device, the lifting rocker arm 83 is hinged to the rocker arm connecting rod 84, the rocker arm connecting rod 84 is fixed on the shaking table 5 through a rocker arm movable hole 13 of the upper shell 1 of the film washing device, and the lifting rocker arm 83 is fixed on the open-end rocker arm 81 through a belt guide rail motor 73 of the electric device. The transmission device comprises a synchronizing wheel 91, a synchronizing wheel belt 92, an open-end synchronizing wheel fixing shaft 93 and a fixed-end synchronizing wheel fixing shaft 94, wherein the synchronizing wheel 91 is respectively sleeved on the open-end synchronizing wheel fixing shaft 93 and the fixed-end synchronizing wheel fixing shaft 94, and the open-end synchronizing wheel fixing shaft 93 and the fixed-end synchronizing wheel fixing shaft 94 are respectively sleeved on the open-end synchronizing wheel fixing shaft 93 and the fixed-end synchronizing wheel fixing shaft 93.
The utility model provides a membrane system is washed to full-automatic high-efficient western blot that biomedical basic experiment used, is modular structure, by wash membrane device epitheca 1, wash membrane device base 2, liftable trade liquid device 3, wash membrane box 4, shaking table 5, water pump unit, electric actuator, rocking arm device, transmission and control panel 10 and constitute.
The membrane washing device upper shell 1 is similar to a cuboid structure, a rocker arm moving hole 13 and a liquid changing rod lifting hole 12 are sequentially arranged in the middle area of the top surface of the membrane washing device upper shell, an inclined plane is arranged at the front end of one side of the rocker arm moving hole 13, a control panel mounting end 11 is arranged on the inclined plane, a waste liquid bottle connecting end 14 and a washing liquid bottle connecting end 15 are arranged on the opposite surface, the waste liquid bottle connecting end 14 is connected with a waste liquid bottle through a hose, and the washing liquid bottle connecting end 15 is connected with a washing liquid bottle through a hose.
Washing membrane device base 2 be similar washing membrane device epitheca 1 structure, set gradually biax motor mount pad 21 on it, first synchronizing wheel unable adjustment base 22, unipolar motor installation base 23, second synchronizing wheel unable adjustment base 24 and lead screw motor base 28, wherein be provided with first rocking arm guide rail 25 and second rocking arm guide rail 26 between biax motor mount pad 21 and the first synchronizing wheel unable adjustment base 22, be provided with third rocking arm guide rail 27 between unipolar motor installation base 23 and the second synchronizing wheel unable adjustment base 24.
The lifting liquid-changing device 3 is composed of a lifting liquid-changing rod 31 and a liquid-changing rod fixing frame 32. The liftable liquid-changing rod 31 is 7-shaped, the front end and the bottom of the liftable liquid-changing rod are respectively provided with a first liquid injection port 311/b313, a first liquid discharge port 312/b314 and a liquid-changing rod screw hole 315, wherein the first liquid injection port 311, the second liquid injection port 313, the first liquid discharge port 312 and the second liquid discharge port 314 are respectively communicated with each other through hole channels. Two sides of the lower end of the liquid changing rod are provided with limiting steps to prevent the liftable liquid changing rod 31 from separating from the upper shell 1 of the membrane washing device when rising. The liquid change rod fixing frame 32 is U-shaped, and the opening is fixed on the membrane washing device upper shell 1 upwards, so that the second liquid injection port 313 and the second liquid discharge port 314 are prevented from colliding with the membrane washing box 4 when the liftable liquid change rod 31 descends.
The membrane washing box 4 is of a cuboid structure and is provided with an opening at the top end. The washing liquid box is composed of a liquid changing bin 41 and a membrane washing bin 42, wherein a washing liquid circulation hole 43 is arranged on a partition plate between the liquid changing bin 41 and the membrane washing bin 42, and a washing liquid box limiting groove 44 is arranged at the bottom of the membrane washing box 4. In a normal state, the second liquid injection port 313 and the second liquid discharge port 314 of the liquid change lever 31 are disposed inside the liquid change tank 41, which facilitates liquid change. When in use, the membrane washing box 4 is fixed on the shaking table 5. The membrane washing box 4 can have different specification models such as a storehouse, two storehouses and four storehouses according to actual need, wherein adjacent liquid changing storehouses 41 in the multi-storehouse membrane washing box 4 such as two storehouses and four storehouses are all inside through, and lotion can flow to other liquid changing storehouses 41 through one of the liquid changing storehouses 41 and flow to each membrane washing storehouse 42 through a lotion circulation hole 43.
Shaking table 5 be approximate to U word structure, the centre is the capsule 4 place the platform of washing, both sides are the baffle around, wherein place the platform top intermediate position be provided with the anastomotic capsule draw-in groove 51 of the long limit direction of capsule 4 of washing, two capsule draw-in grooves 51 centers are provided with the spacing post 55 of capsule of washing, prevent that capsule 4 of washing from droing at the device during operation. An open end rocker arm fixed clamp arm 52, a first rocker arm connecting end 53 and a first fixed end rocker arm connecting end 54 are arranged below the placing platform.
The membrane washing device upper shell 1, the membrane washing device base 2, the liftable liquid changing device 3, the membrane washing box 4 and the shaking table 5 form an integral appearance piece of the device.
The water pump device consists of a liquid suction water pump 61, a liquid discharge water pump 62 and a water pump fixing frame 63. The water pump 61 and the drainage water pump 62 are the same standard small water pump, the classification is determined by the function, and the water pump fixing frame 63 is a matching component of the water pump 61 and the drainage water pump 62. In the device, the water inlet of the imbibition water pump 61 is connected with the connecting end 15 of the lotion bottle, and the water outlet is connected with the first liquid injection port 311 of the liftable liquid exchange rod 31; the water inlet of the liquid drainage pump 62 is connected with the waste liquid bottle connecting end 14, and the water outlet is connected with the first liquid drainage port 312 of the liftable liquid exchange rod 31;
the electric device consists of a double-shaft motor 71, a single-shaft motor 72, a lead screw motor 73 with a guide rail and a lead screw motor 74. The double-shaft motor 71 is fixed on the double-shaft motor mounting base 21 and is connected with a synchronizing wheel 91 fixed on the first synchronizing wheel fixing base 22 through a synchronizing wheel belt 92 fixed on the open end rocker arm 81; the single-shaft motor 72 is fixed on the single-shaft motor mounting base 23 and is connected with a synchronizing wheel 91 fixed on the second synchronizing wheel fixing base 24 through a synchronizing wheel belt 92 fixed on the fixed end rocker arm 82; the lead screw motor 73 with the guide rail is fixed at the top of the lower side of the open end rocker arm 81; the lead screw motor 74 is fixed to the lead screw motor base 28. The double-shaft motor 71 and the single-shaft motor 72 are responsible for driving the shaking table 5 to swing, the lead screw motor 73 with the guide rail is responsible for jacking the shaking table 5 for a certain angle before finishing liquid drainage, so that waste liquid can be discharged completely, and the lead screw motor 74 is responsible for controlling the lifting liquid change rod 31 to lift.
The rocker arm device consists of an open end rocker arm 81, a fixed end rocker arm 82, a lifting rocker arm 83 and a rocker arm connecting rod 84. The open end swing arm 81 is composed of a high portion and a low portion, and the bottom portion of the open end swing arm is provided with a first synchronous belt fixing hole 811 and a first guide rail sliding hole 812. In the device, the synchronizing wheel belt 92 is fixed in a first synchronizing wheel belt fixing hole 811, the first rocker guide rail 25 and the second rocker guide rail 26 respectively pass through a first guide rail sliding hole 812 at the bottom of the high-low part of the open-end rocker 81, the top of the first rocker guide rail is inserted into the open-end rocker fixing arm 52 of the shaking table 5 through a rocker movable hole 13 corresponding to the upper shell 1 of the film washing device, and the phenomenon that the front end and the rear end of the shaking table 5 are asynchronous to cause the splashing of washing liquid when the shaking table 5 swings left and right is prevented, so that the film washing effect is reduced.
The lower part of the fixed end rocker arm 82 has the same structure as the lower part of the upper part of the open end rocker arm 81, and is provided with a second synchronous belt fixing hole 821 and a second guide rail sliding hole 822, and the difference is that the top of the fixed end rocker arm 82 is provided with a second fixed end rocker arm connecting end 823. In the device, the synchronous belt 92 is fixed in the second synchronous belt fixing hole 821, the third rocker guide 27 passes through the second guide sliding hole 822 at the bottom of the fixed end rocker 82, the second fixed end rocker connecting end 823 at the top passes through the rocker movable hole 13 corresponding to the membrane washing device upper shell 1, and then is connected with the first fixed end rocker connecting end 54 on the shaking table 5 through a standard pin with the diameter of 5 x 20mm, and the shaking table 5 can rotate around the pin axis after connection.
The lifting rocker 83 is similar to a convex structure, the lower end of the lifting rocker is provided with a screw hole 831 and a motor guide rail hole 832 which are matched with the upper guide rail and the screw rod of the screw rod motor 73 with the guide rail, and the top end of the lifting rocker is provided with a second rocker connecting rod connecting end 833. In the device, the lifting rocker 83 can pass through the rocker movable hole 13 corresponding to the membrane washing device upper shell 1 to be connected with the rocker connecting rod 84.
The rocker link 84 is a centrosymmetric H-shaped structure. In the device, a second rocker link connecting end 833 on the lifting rocker 83 and a first rocker link connecting end 53 on the shaking table 5 can be connected through a standard pin with the diameter of 5 x 20mm, so that the shaking table 5 is driven to lift conveniently when the screw motor 73 with the guide rails works.
Example 2:
before use, the touch screen of the control panel 10 is used for setting the swinging time, the swinging distance, the lifting angle after swinging, the running time of the water pump (including liquid injection time and liquid drainage time) and the membrane washing times of the swinging bed 5. When the washing film box is used, the washing film box 4 is taken out firstly, a PVDF film or an NC film to be washed is placed in the washing film cabin 42 of the washing film box 4, then the washing film box 4 is fixed in the washing film box clamping groove 51 of the shaking table 5, and the washing film box limiting column 55 on the shaking table 5 is matched and matched with the washing liquid box limiting groove 44 on the washing film box 4. The liquid change bin 41 faces one end of the liquid change rod 31.
After the device is started, the liftable liquid exchange rod 31 descends, the second liquid injection port 313 extends into the liquid exchange bin 41 of the membrane washing box 4, according to the preset time, the liquid suction pump 61 injects a proper amount of washing liquid in the washing liquid bottle into the liquid exchange bin 41, the washing liquid is uniformly distributed in the liquid exchange bin 41 and the membrane washing bin 42 through the washing liquid circulation holes 43, the liftable liquid exchange rod 31 ascends to be higher than the membrane washing box 4, the shaking table 5 swings left and right at the set frequency, after the swinging time is over, the shaking table is jacked up at a certain angle (default 5 degrees) through the screw motor 73 with the guide rail, a certain time is waited, the waste liquid flows to the liquid exchange bin 41, the liquid exchange rod 31 descends, the second liquid discharge port 314 extends into the bottom of the liquid exchange bin 41, the liquid discharge pump 62 sucks the waste liquid in the liquid exchange bin 41 into the waste liquid bottle, after the set time is reached, the liftable liquid exchange rod 31 ascends, the shaking table 5 descends to the horizontal position, then the above steps are repeated according to the set times, and after all the set times are completed (the last step is not completed), the device alarms to notify an operator. And taking out the PVDF membrane or the NC membrane to be washed to carry out subsequent experimental work.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, and that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (3)
1. The utility model provides a membrane system is washed to full-automatic high-efficient western blot which characterized in that includes:
the membrane washing device comprises a membrane washing device upper shell (1), wherein the membrane washing device upper shell (1) is in a hollow platform shape and is matched with a membrane washing device base (2), and a water pump device, an electric device, a rocker arm device and a transmission device are fixed on the membrane washing device base (2);
the lifting liquid changing device (3) is connected with the water pump device and is arranged close to the membrane washing box (4), the membrane washing box (4) is arranged on the shaking table (5) and can be placed in and out, the shaking table (5) is driven by the rocker arm device, and the electric device drives the rocker arm device through the transmission device;
the electric device and the water pump device are controlled by a control panel (10), and the control panel (10) respectively controls the water pump device and the electric device;
the upper part of the membrane washing device upper shell (1) is provided with a liquid exchange rod lifting hole (12) and a rocker arm movable hole (13), the side wall of the membrane washing device upper shell is provided with a control panel mounting end (11), a waste liquid bottle connecting end (14) and a washing liquid bottle connecting end (15), the control panel mounting end (11) is used for fixing a control panel, and the control panel (10) is connected with a touch screen;
the membrane washing device is characterized in that a double-shaft motor mounting base (21), a first synchronous wheel fixing base (22), a single-shaft motor mounting base (23), a second synchronous wheel fixing base (24), a first rocker guide rail (25), a second rocker guide rail (26), a third rocker guide rail (27) and a lead screw motor base (28) are arranged on the upper portion of the membrane washing device base (2), the first rocker guide rail (25) and the second rocker guide rail (26) are fixed between the double-shaft motor mounting base (21) and the first synchronous wheel fixing base (22), the two double-shaft motor mounting bases (21) are used for fixing double-shaft motors (71) of an electric device, the two first synchronous wheel fixing bases (22) are used for fixing synchronous wheels (91) of a transmission device respectively, the third rocker guide rail (27) is fixed between the single-shaft motor mounting base (23) and the second synchronous wheel fixing base (24), and the lead screw motor base (28) is used for fixing a liftable liquid changing device (3);
the liftable liquid replacing device (3) comprises a liftable liquid replacing rod (31) and a liftable liquid replacing rod fixing frame (32), wherein the liftable liquid replacing rod fixing frame (32) is fixed at the bottom of the liftable liquid replacing rod (31) through a liquid replacing rod screw rod hole (315) and is driven by a screw rod motor (74) of an electric device, the liftable liquid replacing rod (31) is 7-shaped, the upper part of the liftable liquid replacing rod is positioned at the upper part of the membrane washing box (4), and is provided with a second liquid injection port (313) and a second liquid discharge port (314), and the bottom of the liftable liquid replacing rod fixing frame is provided with a first liquid injection port (311) and a first liquid discharge port (312);
the membrane washing box (4) is provided with a liquid changing bin (41) and a membrane washing bin (42), the bottom of the membrane washing box is provided with a membrane washing box limiting groove (44), and the inside of the membrane washing box is provided with a washing liquid circulation hole (43);
the shaking table is characterized in that a membrane washing box clamping groove (51) and a membrane washing box limiting column (55) are arranged on the upper portion of the shaking table (5), an open-end rocker arm fixing clamping arm (52), a first rocker arm connecting rod connecting end (53) and a first fixed-end rocker arm connecting end (54) are arranged on the lower portion of the shaking table, the two membrane washing box clamping grooves (51) are used for clamping the membrane washing box (4), the membrane washing box limiting column (55) is matched with the membrane washing box limiting groove (44) of the membrane washing box (4), the two open-end rocker arm fixing clamping arms (52) are used for clamping an open-end rocker arm (81) of the rocker arm device, the first rocker arm connecting end (53) is connected with a rocker arm connecting rod (84) of the rocker arm device, and the first fixed-end rocker arm connecting end (54) is connected with a fixed-end rocker arm (82) of the rocker arm device;
the electric device comprises a double-shaft motor (71), a single-shaft motor (72), a lead screw motor (73) with a guide rail and a lead screw motor (74), wherein the double-shaft motor (71) is fixed on a double-shaft motor mounting base (21), the single-shaft motor (72) is fixed on a single-shaft motor mounting base (23), the lead screw motor (73) with the guide rail is fixed on an open end rocker arm (81) of the rocker arm device, and the lead screw motor (74) is fixed on a lead screw motor base (28);
the rocking arm device comprises an open end rocking arm (81), a fixed end rocking arm (82), a lifting rocking arm (83) and a rocking arm connecting rod (84), wherein the open end rocking arm (81) is L-shaped, a first synchronous wheel belt fixing hole (811) and a first guide rail sliding hole (812) are formed in the bottom of the open end rocking arm, the first synchronous wheel belt fixing hole (811) penetrates through a synchronous wheel belt (92) of the transmission device, the first guide rail sliding hole (812) is respectively sleeved on a first rocking arm guide rail (25) and a second rocking arm guide rail (26) of the film washing device base (2), the fixed end rocking arm (82) is linear, a second synchronous wheel belt fixing hole (821) and a second guide rail sliding hole (822) are formed in the bottom of the fixed end rocking arm, the lifting rocking arm (83) is hinged to the rocking arm connecting rod (84), the second guide rail sliding hole (822) is sleeved on a third rocking arm guide rail (27) of the film washing device base (2), the second synchronous wheel belt fixing hole (821) penetrates through the synchronous wheel belt (92) of the transmission device, the lifting rocking arm (83) and the rocking arm (84) are fixed on the upper shell of the swing arm (81) through a movable lead screw (73), and the lifting rocking arm (73) is fixed on the electric rocking arm (13).
2. The fully automatic high-efficiency western blot membrane washing system according to claim 1, wherein the water pump device comprises a liquid suction water pump (61) and a liquid discharge water pump (62), the liquid suction water pump (61) and the liquid discharge water pump (62) are respectively fixed on a water pump fixing frame (63), and the water pump fixing frame (63) is fixed on the membrane washing device base (2).
3. The fully automatic high efficiency western blot membrane washing system according to claim 1, wherein the transmission device comprises a synchronizing wheel (91), a synchronizing wheel belt (92), an open end synchronizing wheel fixing shaft (93) and a fixed end synchronizing wheel fixing shaft (94), the synchronizing wheel (91) is respectively sleeved on the open end synchronizing wheel fixing shaft (93) and the fixed end synchronizing wheel fixing shaft (94).
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| CN201611187775.XA CN106733886B (en) | 2016-12-20 | 2016-12-20 | Full-automatic high-efficient western blot membrane washing system |
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| CN201611187775.XA CN106733886B (en) | 2016-12-20 | 2016-12-20 | Full-automatic high-efficient western blot membrane washing system |
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| CN106733886B true CN106733886B (en) | 2023-03-24 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108188094B (en) * | 2018-02-05 | 2024-02-23 | 朱宏明 | Automatic film washing machine for western blot test |
| CN108453085A (en) * | 2018-03-30 | 2018-08-28 | 倪华静 | Western Blot intelligently wash film device |
| CN109807106A (en) * | 2019-03-21 | 2019-05-28 | 安图实验仪器(郑州)有限公司 | Full automatic support disc type slide shakes wiper mechanism |
| CN110850098B (en) * | 2019-11-21 | 2023-11-14 | 中国人民解放军西部战区总医院 | Automatic film washing device for Western blot experiment |
| CN112666354B (en) * | 2020-12-15 | 2022-06-17 | 韦克斯科技(北京)有限公司 | Full-automatic western blot processing system |
| CN114453331B (en) * | 2022-02-11 | 2023-06-23 | 四川大学华西医院 | Automatic membrane washing device for Western Blot experiment |
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| AU635008B2 (en) * | 1989-12-13 | 1993-03-11 | Genelabs Diagnostics Pte Ltd | Analytical apparatus and method for automated blot assay |
| CN2775658Y (en) * | 2005-02-24 | 2006-04-26 | 穆海东 | Biological chip reaction instrument |
| CN101923096B (en) * | 2009-06-12 | 2013-01-02 | 上海迅达医疗仪器有限公司 | Automatic westem blot analyzer |
| CN202114037U (en) * | 2011-05-26 | 2012-01-18 | 合肥安弗朋德生物技术有限公司 | Swing type full-automatic cleaner for biological experiments |
| CN202256360U (en) * | 2011-09-27 | 2012-05-30 | 深圳市亚辉龙生物科技有限公司 | Full-automatic washing mechanism |
| CN102507964B (en) * | 2011-10-31 | 2013-12-18 | 上海裕隆生物科技有限公司 | Full-automatic reactor |
| CN103616526B (en) * | 2013-12-16 | 2015-10-28 | 深圳市亚辉龙生物科技股份有限公司 | A kind of full-automatic immunoblotting assay instrument and using method thereof and application |
| CN205176039U (en) * | 2015-06-09 | 2016-04-20 | 南京鼎昊园生物科技有限公司 | Full -automatic albumen trace appearance |
| CN206492747U (en) * | 2016-12-20 | 2017-09-15 | 上海长海医院 | A kind of full automatic high efficiency western blot wash membranous system |
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