CN106706586A - Copper ion fluorescence detection test paper based on nano zinc oxide as well as preparation method and detection method thereof - Google Patents
Copper ion fluorescence detection test paper based on nano zinc oxide as well as preparation method and detection method thereof Download PDFInfo
- Publication number
- CN106706586A CN106706586A CN201710011987.0A CN201710011987A CN106706586A CN 106706586 A CN106706586 A CN 106706586A CN 201710011987 A CN201710011987 A CN 201710011987A CN 106706586 A CN106706586 A CN 106706586A
- Authority
- CN
- China
- Prior art keywords
- copper ion
- fluorescence detection
- solution
- detection test
- test
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses copper ion fluorescence detection test paper based on nano zinc oxide as well as a preparation method and detection method thereof. The preparation method comprises the following steps: (1) preparation of nano zinc oxide dispersion: dissolving zinc salt and alkali in a molar ratio of (0.25-4):1 in alcohol respectively to obtain an alcohol solution of zinc salt and an alcohol solution of alkali; heating and stirring the alcohol solution of zinc salt for 5-20min at 50-70 DEG C; adding the alcohol solution of alkali, and further stirring for 10-30min at 50-70 DEG C; and cooling to room temperature to obtain nano zinc oxide dispersion; and (2) preparation of test paper: soaking an adsorption layer in the nano zinc oxide dispersion obtained in the step (1) for 1-10min, and wetting sufficiently; and after that, taking out the soaked adsorption layer, and drying to obtain copper ion fluorescence detection test paper.
Description
Technical field
The invention belongs to copper ion detection technique field, and in particular to a kind of copper ion fluorescence inspection based on nano zine oxide
Test paper and preparation method thereof and detection method.
Background technology
Copper is a kind of typical heavy metal element, at the same be also in human body content be only second to it is micro necessary to the life of iron
Element.Used as more than 30 cofactorses for planting important zymoprotein, copper ion participates in numerous important vital movements.However, excessive
The intake of copper ion can cause a series of health problems, such as the organ-tissue such as ischemic heart disease, anaemia and kidney, bone
Lesion.Moreover, the copper ion of too high amount also can cause strong influence to aquatile in water body, when organism synthesis
Metal hydrosulfide albumen can not meet with enter cell copper ion combined the need for when, copper ion will be with other biological molecule phase
Interaction, causes denaturation to be poisoned, and bioconcentration can also make the mankind in biological chain top be subjected to its evil.Just because of such as
This, China《Standards for drinking water quality》Copper content must not exceed 1 mg/ L in regulation Drinking Water（15.7 μmol /
L）；《Water environment quality standard》Content of copper ion must not exceed 1 mg in II, III, IV and V class earth's surface waters of regulation
/L（15.7 μmol/ L）.Meanwhile, heavy metal copper also by《Heavy metal pollution integrated control " 12 " is planned》It is classified as emphasis dirty
Dye thing.Copper ion pollution is how related to the exploitation and smelting of copper mine.Especially wet process iron-smelting copper and high-purity electrolytic copper refining, its
The industrial wastewater of a large amount of copper ions can be produced.How copper ion is carried out to industrial water drainage, related waters and household drinking water
Fast and effectively in situ detection, be to realize to the effectively protection of water body Cu-W ore deposit and one of the key administered.
Although copper ion present in water body can make the blueness of water body expression characteristicses, when copper ion concentration is less than in water body
During 1 mmol/L, naked eyes have been difficult to distinguish.And the concentration of 1 mmol/ L is still far above national standard decades of times.Relative to quantitative
Accurately, but electrochemical process, spectrophotometer method, the Atomic absorption of numerous and diverse operation or high cost or costly and cumbersome instrument are needed
Spectroscopic methodology and inductively coupled plasma transmitting spectrometry, to water in Test paper more conducively investigation in the wild and daily life
The need for body carries out quick in situ detection.
At present, from Macherey-Nagel companies of Germany, it is produced the most import of domestic commercially available copper ion test paper
Product are expensive, every hundred RMB of test paper price about 300, and it is only capable of differentiating copper ion concentration scope in water body, it is impossible to
Realize quantitative analysis.Its lowest detection is limited to 10 mg/L simultaneously, still far above national standard threshold value.Based on this, it is necessary to develop
A kind of cheap, sensitivity is high, be easy to carry, and it is to be capable of achieving to carry out quantitative determination to copper ion not need XRF
Test paper.
The content of the invention
The first object of the present invention is to provide a kind of copper ion fluorescence detection test based on nano zine oxide, the present invention
The second purpose be that a kind of preparation method of the copper ion fluorescence detection test based on nano zine oxide is provided, of the invention
Three purposes are to provide a kind of detection method of the copper ion fluorescence detection test based on nano zine oxide.
The first object of the present invention is achieved in that described copper ion fluorescence detection test is in nanometer by adsorption layer
Obtained after soaking and drying in zinc oxide fluid dispersion.
The second object of the present invention is achieved in that and comprises the following steps：
1）The preparation of nano zinc oxide fluid dispersion：It is 0.25 ~ 4 by mol ratio：1 zinc salt and alkali is dissolved in alcohol respectively, is obtained
The alcoholic solution and alkali alcosol of zinc salt；By the alcoholic solution of zinc salt at 50 ~ 70 DEG C the min of heating stirring 5 ~ 20, then by alkali
Alcoholic solution is added, and continues to stir 10 ~ 30 min at 50 ~ 70 DEG C, is subsequently cooled to room temperature and is obtained nano zinc oxide fluid dispersion；
2）The making of test paper：Adsorption layer is immersed in step（1）1 ~ 10 min in the nano zinc oxide fluid dispersion for obtaining, fully leaching
Profit, then takes out the adsorption layer after immersion, and copper ion fluorescence detection test is obtained after drying.
The third object of the present invention is achieved in that described detection method, specially will be described for qualitative detection
Copper ion fluorescence detection test is immersed in 1 ~ 2 s in solution to be measured, the solution for getting rid of and being remained on test paper is then taken out, in uviol lamp
Lower observation, test paper yellow fluorescence weakens, you can to detect and contain copper ion in solution to be measured.
Beneficial effects of the present invention：
1st, copper ion fluorescence detection test of the invention is easy to use, is quick on the draw, with low cost, makes simple, and need not
The participation of professional and large-scale detecting instrument be capable of achieving copper ion is carried out quick quantitative and semi-quantitative detection and
Quantitative determination, easy to carry, detection range is wide, strong applicability.
2nd, the invention provides a kind of copper ion fluorescence detection test based on nano zine oxide, born using on Test paper
Fluorescence reaction of the nano granular of zinc oxide of load to copper ion, it is possible to achieve the concentration range to copper ion in water body carries out semidefinite
Amount detection, aids in related software, can further realize quantitative determination.
3rd, zinc oxide used in the present invention is a kind of cheap wide bandgap semiconductor, and itself has room temperature fluorescence
Characteristic.Zinc oxide with particular surface state and pattern has specific fluorescent quenching characteristic to copper ion, by determining
The relation of copper ion concentration and zinc oxide luminescent intensity, you can determine the concentration of copper ion in testing sample.Compared to body phase material
Material, nano zine oxide has more surfaces and abundant surface defect, so that with fluorescence intensity higher, being more suitable for should
For fluorescent quenching.Copper ion fluorescence detection test based on nano zine oxide of the invention is solved quantitatively to be examined in the prior art
Must be by the problem of costly and cumbersome XRF during survey；Dispersion in the prior art is solved simultaneously
Nano zine oxide must be transported, carries and preserved, and test process consumption is big, a series of problems, such as with high costs, realizes house
To the quick detection of copper ion under front yard and wild environment, simple and convenient, zinc oxide consumption is small, and detection is quick on the draw, efficiency high,
Strong applicability.
Brief description of the drawings
Fig. 1 is the X-ray diffractogram of nano zinc oxide fluid dispersion prepared by the embodiment of the present invention 3；
Fig. 2 is the transmission electron microscopy figure of nano zinc oxide fluid dispersion prepared by the embodiment of the present invention 3；
Fig. 3 is the photo of the test paper under fluorescent lamp and uviol lamp of the copper ion solution impregnated of various concentrations in embodiment 6；
Fig. 4 is grey scale change curves of the Fig. 3 impregnated of the photo of the test paper under uviol lamp of the copper ion solution of various concentrations；
Fig. 5 is the linear relationship in embodiment 7 between copper ion concentration and gray value；
Fig. 6 is the photo of the different metal salting liquid of the test paper dipping same concentrations prepared by embodiment 3.
Specific embodiment
With reference to embodiment and accompanying drawing, the present invention is further illustrated, but the present invention is subject to never in any form
Limitation, based on present invention teach that any conversion or replacement made, belong to protection scope of the present invention.
Copper ion fluorescence detection test based on nano zine oxide of the present invention, described copper ion fluoroscopic examination examination
Paper obtains adsorption layer in nano zinc oxide fluid dispersion after soaking and drying.
A kind of preparation method of the copper ion fluorescence detection test based on nano zine oxide, comprises the following steps：
1）The preparation of nano zinc oxide fluid dispersion：It is 0.25 ~ 4 by mol ratio：1 zinc salt and alkali is dissolved in alcohol respectively, is obtained
The alcoholic solution and alkali alcosol of zinc salt；By the alcoholic solution of zinc salt at 50 ~ 70 DEG C the min of heating stirring 5 ~ 20, then by alkali
Alcoholic solution is added, and continues to stir 10 ~ 30 min at 50 ~ 70 DEG C, is subsequently cooled to room temperature and is obtained nano zinc oxide fluid dispersion；
2）The making of test paper：Adsorption layer is immersed in step（1）1 ~ 10 min in the nano zinc oxide fluid dispersion for obtaining, fully leaching
Profit, then takes out the adsorption layer after immersion, and copper ion fluorescence detection test is obtained after drying.
Step（1）Described in zinc salt be one or more in zinc sulfate, zinc acetate, zinc chloride, zinc nitrate.
Step（1）Described in alkali be one or more in lithium hydroxide, NaOH, potassium hydroxide, described alcohol
It is one or more in methyl alcohol, ethanol, propyl alcohol, butanol, amylalcohol, hexanol.
Step（2）Described in adsorption layer be qualitative filter paper, printing paper or lens wiping paper.
Step（2）Described in drying be to be dried up using hair-dryer.
A kind of detection method of the copper ion fluorescence detection test based on nano zine oxide, described detection method is qualitative
Detection, is specially immersed in 1 ~ 2 s in solution to be measured by described copper ion fluorescence detection test, then takes out and gets rid of on test paper
The solution of residual, observes under uviol lamp, and test paper yellow fluorescence weakens, you can to detect and contain copper ion in solution to be measured.
A kind of detection method of the copper ion fluorescence detection test based on nano zine oxide, described detection method is semidefinite
Amount detection, comprises the following steps：
1）With copper chloride as copper ion source, the copper ion standard liquid of various concentrations is configured, examined using described copper ion fluorescence
Test paper soaks 1 ~ 2s in the copper ion standard liquid of various concentrations respectively, then takes out, and gets rid of what is remained on each test paper
Solution, observes under uviol lamp, obtains the fluorescence detection test standard specimen of the copper ion of various concentrations；
2）Described copper ion fluorescence detection test is immersed in 1 ~ 2 s in solution to be measured, is then taken out to be got rid of and remained on test paper
Solution, observed under uviol lamp, and enter under uviol lamp with the fluorescence detection test standard specimen of the copper ion of above-mentioned various concentrations
The comparing of row fluorescence intensity, you can obtain the concentration range of copper ion in solution to be measured.
A kind of detection method of the copper ion fluorescence detection test based on nano zine oxide, described detection method is quantitative
Detection, comprises the following steps：
1）With copper chloride as copper ion source, the copper ion standard liquid of various concentrations is configured, examined using described copper ion fluorescence
Test paper soaks 1 ~ 2s in the copper ion standard liquid of various concentrations respectively, then takes out, and gets rid of what is remained on each test paper
Solution, observes under uviol lamp, obtains the fluorescence detection test standard specimen of the copper ion of various concentrations；
2）To step（1）In the fluorescence detection test standard specimen of the copper ion of various concentrations that obtains taken pictures under uviol lamp,
And grey scale change curve is derived photo by the grey scale change curve in test paper region in software analysis acquisition photo, obtain
Gray value under each copper ion concentration, matched curve, so as to obtain the linear relationship of logarithm value between concentration and gray value；
3）Described copper ion fluorescence detection test is immersed in 1 ~ 2 s in solution to be measured, is then taken out to be got rid of and remained on test paper
Solution, its fluorescence is observed under uviol lamp；
4）To step（3）In the copper ion fluorescence detection test of solution to be measured that obtains taken pictures under uviol lamp, and will shine
Piece is processed by software analysis and obtains gray value, then by step（2）In linear relationship, realization copper ion solution is quantified
Test.
Step（1）Described in various concentrations copper ion standard liquid for copper of the concentration in the range of 10 ~ 1000 μM from
Substandard solution.
Described standard liquid is standard aqueous solution.
Described software is ImageJ softwares.
Embodiment 1
A kind of preparation method of the copper ion fluorescence detection test based on nano zine oxide, comprises the following steps：
1）The preparation of nano zinc oxide fluid dispersion：It is 0.25 by mol ratio：1 zinc salt and alkali is dissolved in alcohol respectively, obtains zinc
The alcoholic solution and alkali alcosol of salt；By the alcoholic solution of zinc salt at 50 DEG C heating stirring 5min, then alkali alcosol is added
Enter, continuation stirs 10min at 50 DEG C, is subsequently cooled to room temperature and obtains nano zinc oxide fluid dispersion；Described zinc salt is sulphur
Sour zinc；Described alkali is NaOH；Described alcohol is methyl alcohol；
2）The making of test paper：Adsorption layer is immersed in step（1）1min in the nano zinc oxide fluid dispersion for obtaining, fully infiltration,
Then the adsorption layer after immersion is taken out, copper ion fluorescence detection test is obtained after being dried up using hair-dryer；Described absorption
Layer is qualitative filter paper.
Embodiment 2
A kind of preparation method of the copper ion fluorescence detection test based on nano zine oxide, comprises the following steps：
1）The preparation of nano zinc oxide fluid dispersion：It is 4 by mol ratio：1 zinc salt and alkali is dissolved in alcohol respectively, obtains zinc salt
Alcoholic solution and alkali alcosol；By the alcoholic solution of zinc salt at 70 DEG C the min of heating stirring 20, then alkali alcosol is added,
30 min are stirred in continuation at 70 DEG C, are subsequently cooled to room temperature and are obtained nano zinc oxide fluid dispersion；Described zinc salt is nitric acid
Zinc；Described alkali is lithium hydroxide；Described alcohol is hexanol；
2）The making of test paper：Adsorption layer is immersed in step（1）10 min in the nano zinc oxide fluid dispersion for obtaining, fully leaching
Profit, then takes out the adsorption layer after immersion, and copper ion fluorescence detection test is obtained after drying；Described adsorption layer is wiping mirror
Paper.
Embodiment 3
A kind of preparation method of the copper ion fluorescence detection test based on nano zine oxide, comprises the following steps：
1）The preparation of nano zinc oxide fluid dispersion：It is 0.5 by mol ratio：1 zinc salt and alkali is dissolved in volume ratio respectively
1.826：In 1 ethanol, the alcoholic solution and alkali alcosol of zinc salt are obtained；By the alcoholic solution of zinc salt at 60 DEG C heating stirring 10
Min, then adds alkali alcosol, and 20 min are stirred in continuation at 60 DEG C, are subsequently cooled to room temperature and are obtained nano oxidized
Zinc dispersion liquid；Described zinc salt is zinc acetate；Described alkali is potassium hydroxide；Nano zinc oxide fluid dispersion to obtaining is examined
Survey, obtain X-ray diffractogram as shown in Figure 1 and transmission electron microscopy figure is as shown in Figure 2；
2）The making of test paper：Adsorption layer is immersed in step（1）3min in the nano zinc oxide fluid dispersion for obtaining, fully infiltration,
Then the adsorption layer after immersion is taken out, copper ion fluorescence detection test is obtained after being dried up using hair-dryer；Described absorption
Layer is qualitative filter paper, and described qualitative filter paper can be cut into suitable size.
By Fig. 1 and Fig. 2, there is nano zine oxide prepared by the present invention more surfaces and abundant surface to lack
Fall into, with fluorescence intensity higher, be highly suitable to be applied for fluorescent quenching, be suitable as detect copper ion and for copper from
The preparation of sub- fluorescence detection test.
Embodiment 4
A kind of preparation method of the copper ion fluorescence detection test based on nano zine oxide, comprises the following steps：
1）The preparation of nano zinc oxide fluid dispersion：It is 1 by mol ratio：It is 2 that 1 zinc salt and alkali is dissolved in volume ratio respectively：The third of 1
In alcohol, the alcoholic solution and alkali alcosol of zinc salt are obtained；By the alcoholic solution of zinc salt at 65 DEG C the min of heating stirring 15, then will
Alkali alcosol is added, and 18 min are stirred in continuation at 65 DEG C, are subsequently cooled to room temperature and are obtained nano zinc oxide fluid dispersion；Institute
The zinc salt stated is zinc acetate；Described alkali is potassium hydroxide；
2）The making of test paper：Adsorption layer is immersed in step（1）5min in the nano zinc oxide fluid dispersion for obtaining, fully infiltration,
Then the adsorption layer after immersion is taken out, copper ion fluorescence detection test is obtained after drying；Described adsorption layer is qualitative filter
Paper, described qualitative filter paper can be cut into suitable size.
Embodiment 5
The copper ion fluorescence detection test prepared using embodiment 1 carries out qualitative detection, specially by described copper ion fluorescence
Test paper is immersed in 1 s in solution to be measured, then takes out the solution for getting rid of and being remained on test paper, is observed under uviol lamp, test paper
Yellow fluorescence weakens, and to detect and contain copper ion in solution to be measured.
Embodiment 6
The copper ion fluorescence detection test prepared using embodiment 3 carries out half-quantitative detection, comprises the following steps：
1）With copper chloride as copper ion source, configuration concentration is 1000,300,100,40,10 μM of copper ion standard liquid, is used
Described copper ion fluorescence detection test soaks 2s in the copper ion standard liquid of various concentrations respectively, then takes out, and gets rid of
The solution remained on each test paper, obtains the fluorescence detection test standard specimen of the copper ion of various concentrations；Impregnated of various concentrations
The photo of test paper after copper ion solution under daylight and ultraviolet light is as shown in Figure 3；
2）Described copper ion fluorescence detection test is immersed in 2 s in solution to be measured, is then taken out and is got rid of what is remained on test paper
Solution, observes under ultraviolet light, and and step（1）The fluorescence detection test standard specimen of the copper ion of middle various concentrations is ultraviolet
The comparing of fluorescence intensity is carried out under light irradiation, you can obtain the concentration range of copper ion in solution to be measured.
By Fig. 3 it is observed that in the sunlight, Test paper is white, and indifference；Under ultraviolet light, leaching
The test paper for applying 10 μM of copper ions has macroscopic yellow fluorescence, with the lifting of surveyed copper ion concentration, the Huang of test paper
Color fluorescence intensity gradually weakens, and Test paper hardly shows fluorescence to 1000 μM of copper ion.Such that it is able to realize to from 10
To the qualitative detection of 1000 μM of copper ion solution, and can determine that the interval of copper ion concentration contained by detected solution.And detect
15.7 μm of ol/L of the detection threshold value that the test limit of test paper has been up to state standards.
Embodiment 7
Photo to the copper ion solution of various concentrations that is obtained in embodiment 6 after detection paper under ultraviolet light is carried out
Qualitative analysis, photo is obtained the grey scale change curve in test paper region in photo, as Fig. 4 by ImageJ software analysis；Will
Grey scale change curve is derived, and obtains the gray value under each copper ion concentration, matched curve, so as to obtain copper ion concentration with ash
Linear relationship between angle value, as Fig. 5；
Again by the step of embodiment 6（2）In the copper ion fluorescence detection test of solution to be measured that obtains carried out under ultraviolet light
Take pictures, then photo is processed by ImageJ software analysis and obtains gray value, then by logarithm value between concentration and gray value
Linear relationship, realize to the quantitative test of copper ion solution.Described gray value is Integrate gray value.
By the present embodiment it is observed that the copper ion of various concentrations makes test paper have the same of different fluorescence intensities
When also make it have different gray values, and gray value caused by each concentration copper ion has notable difference.By each concentration
Lower gray value and concentration is depicted as curve, to being tested the copper ion solution from 10 to 1000 μM, the gray scale of test paper and copper from
Sub- log concentration value has good linear relationship, goodness of fit R2More than 0.9740.According to the curve, it is possible to achieve to solution
The fast quantification test of middle copper ion concentration, and do not need the participation of fluorophotometric instrument.
Embodiment 8
So that two hydration dichlorides are violent, two hydration dichloride copper, six hydration nickel sulfate, Zinc diacetate dihydrate, six hydration cobaltous dichlorides,
Plumbi nitras, Aluminium chloride hexahydrate, sodium sulphate, potassium hydroxide, barium nitrate, four nitric hydrate cadmiums, six hydrated chromium trichlorides, five hydrations
Butter of tin configures 100 μM of Mn2+、Cu2+、Ni2+、Zn2+、Co2+、Pb2+、Ca2+、Al3+、Na+、K+、Ba2+、Cd2+、Cr3+、Sn4+
Solution.Copper ion fluorescence detection test made by embodiment 3 is immersed in above-mentioned standard solution and gets rid of residual after 1.5s
Solution, it is as shown in Figure 6 impregnated of photo of the test paper after above-mentioned standard solution under daylight and ultraviolet light.First, soak
The test paper of each solution of stain is white in the sunlight, and under ultraviolet light, each test paper has the different Huang of certain intensity
Color fluorescence, and it is minimum impregnated of the Test paper fluorescence intensity of copper ion.Meanwhile, can equally draw when metal ion solution is configured
Enter Cl-、SO4 2-、OH-、NO3 -Deng anion, and its fluorescent quenching characteristic to test paper is far below copper ion.Illustrate to be filtered
Detection of the paper to copper ion has certain selectivity.
Claims (10)
1. a kind of copper ion fluorescence detection test based on nano zine oxide, it is characterised in that described copper ion fluoroscopic examination examination
In nano zinc oxide fluid dispersion be dried to obtain adsorption layer by immersion by paper.
2. a kind of preparation method of the copper ion fluorescence detection test based on nano zine oxide according to claim 1, its
It is characterised by comprising the following steps：
1）The preparation of nano zinc oxide fluid dispersion：It is 0.25 ~ 4 by mol ratio：1 zinc salt and alkali is dissolved in alcohol respectively, is obtained
The alcoholic solution and alkali alcosol of zinc salt；By the alcoholic solution of zinc salt at 50 ~ 70 DEG C the min of heating stirring 5 ~ 20, then by alkali
Alcoholic solution is added, and continues to stir 10 ~ 30 min at 50 ~ 70 DEG C, is subsequently cooled to room temperature and is obtained nano zinc oxide fluid dispersion；
2）The making of test paper：Adsorption layer is immersed in step（1）1 ~ 10 min in the nano zinc oxide fluid dispersion for obtaining, fully leaching
Profit, then takes out the adsorption layer after immersion, and copper ion fluorescence detection test is obtained after drying.
3. the preparation method of the copper ion fluorescence detection test based on nano zine oxide according to claim 2, its feature
It is step（1）Described in zinc salt be one or more in zinc sulfate, zinc acetate, zinc chloride, zinc nitrate.
4. the preparation method of the copper ion fluorescence detection test based on nano zine oxide according to claim 2, its feature
It is step（1）Described in alkali be one or more in lithium hydroxide, NaOH, potassium hydroxide, described alcohol is first
One or more in alcohol, ethanol, propyl alcohol, butanol, amylalcohol, hexanol.
5. the preparation method of the copper ion fluorescence detection test based on nano zine oxide according to claim 2, its feature
It is step（2）Described in adsorption layer be qualitative filter paper, printing paper or lens wiping paper.
6. a kind of detection method of the copper ion fluorescence detection test based on nano zine oxide according to claim 1, its
It is characterised by that for qualitative detection, specially be immersed in for described copper ion fluorescence detection test to be measured molten by described detection method
1 ~ 2s in liquid, then takes out the solution for getting rid of and being remained on test paper, is observed under uviol lamp, and test paper yellow fluorescence weakens, you can inspection
Measure and contain copper ion in solution to be measured.
7. a kind of detection method of the copper ion fluorescence detection test based on nano zine oxide according to claim 1, its
It is characterised by that described detection method, for half-quantitative detection, is comprised the following steps：
1）With copper chloride as copper ion source, the copper ion standard liquid of various concentrations is configured, examined using described copper ion fluorescence
Test paper soaks 1 ~ 2s in the copper ion standard liquid of various concentrations respectively, then takes out, and gets rid of what is remained on each test paper
Solution, observes under uviol lamp, obtains the fluorescence detection test standard specimen of the copper ion of various concentrations；
2）Described copper ion fluorescence detection test is immersed in 1 ~ 2s in solution to be measured, is then taken out and is got rid of what is remained on test paper
Solution, observes, and carried out under uviol lamp with the fluorescence detection test standard specimen of the copper ion of above-mentioned various concentrations under uviol lamp
The comparing of fluorescence intensity, you can obtain the concentration range of copper ion in solution to be measured.
8. a kind of detection method of the copper ion fluorescence detection test based on nano zine oxide according to claim 1, its
It is characterised by that described detection method, for quantitative determination, is comprised the following steps：
1）With copper chloride as copper ion source, the copper ion standard liquid of various concentrations is configured, examined using described copper ion fluorescence
Test paper soaks 1 ~ 2s in the copper ion standard liquid of various concentrations respectively, then takes out, and gets rid of what is remained on each test paper
Solution, observes under uviol lamp, obtains the fluorescence detection test standard specimen of the copper ion of various concentrations；
2）To step（1）In the fluorescence detection test standard specimen of the copper ion of various concentrations that obtains clapped under ultraviolet light
According to, and photo is derived grey scale change curve by the grey scale change curve in test paper region in software analysis acquisition photo, obtain
Obtain the gray value under each copper ion concentration, matched curve, so as to obtain the linear relationship of logarithm value between concentration and gray value；
3）Described copper ion fluorescence detection test is immersed in 1 ~ 2 s in solution to be measured, is then taken out to be got rid of and remained on test paper
Solution, its fluorescence is observed under uviol lamp；
4）To step（3）In the copper ion fluorescence detection test of solution to be measured that obtains taken pictures under ultraviolet light, and
Photo is processed by software analysis and obtains gray value, then by step（2）In linear relationship, realize to copper ion solution
Quantitative test.
9. the detection method of the copper ion fluorescence detection test based on nano zine oxide according to claim 8, its feature
It is step（1）Described in various concentrations copper ion standard liquid be copper ion mark of the concentration in the range of 10 ~ 1000 μM
Quasi- solution.
10. the detection method of the copper ion fluorescence detection test based on nano zine oxide according to claim 8, its feature
It is that described software is ImageJ softwares.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710011987.0A CN106706586B (en) | 2017-01-09 | 2017-01-09 | A kind of copper ion fluorescence detection test based on nano zine oxide and preparation method thereof and detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710011987.0A CN106706586B (en) | 2017-01-09 | 2017-01-09 | A kind of copper ion fluorescence detection test based on nano zine oxide and preparation method thereof and detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106706586A true CN106706586A (en) | 2017-05-24 |
| CN106706586B CN106706586B (en) | 2019-03-08 |
Family
ID=58907954
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710011987.0A Expired - Fee Related CN106706586B (en) | 2017-01-09 | 2017-01-09 | A kind of copper ion fluorescence detection test based on nano zine oxide and preparation method thereof and detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106706586B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109142294A (en) * | 2018-08-02 | 2019-01-04 | 江苏大学 | A kind of portable arginine detection device and detection method |
| CN114850487A (en) * | 2022-03-19 | 2022-08-05 | 昆明理工大学 | Preparation method of suspension containing nano-copper/nano-zinc oxide composite particles |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1471562A (en) * | 2000-09-22 | 2004-01-28 | �Ʒ� | Optically variable pigments and foils with enhanced color shifting properties |
-
2017
- 2017-01-09 CN CN201710011987.0A patent/CN106706586B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1471562A (en) * | 2000-09-22 | 2004-01-28 | �Ʒ� | Optically variable pigments and foils with enhanced color shifting properties |
Non-Patent Citations (4)
| Title |
|---|
| ANIL H. GORE等: "《Highly Selective and Sensitive Recognition of Cobalt(II) Ions Directly in Aqueous Solution Using Carboxyl-Functionalized CdS Quantum Dots as a Naked Eye Colorimetric Probe:Applications to Environmental Analysis》", 《APPLIED MATERIALS & INTERFACES》 * |
| MARIA TERESA FERN´ANDEZ-ARG¨UELLES等: "《Surface-modified CdSe quantum dots for the sensitive and selective determination of Cu(II) in aqueous solutions by luminescent measurements》", 《ANALYTICA CHIMICA ACTA》 * |
| MASILAMANY KONESWARAN等: "《l-Cysteine-capped ZnS quantum dots based fluorescence sensor for Cu2+ ion》", 《SENSORS AND ACTUATORS B: CHEMICAL》 * |
| 王妍等: "《基于硫化镉量子点的荧光猝灭效应测定微量钴离子》", 《理化检验-化学分册》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109142294A (en) * | 2018-08-02 | 2019-01-04 | 江苏大学 | A kind of portable arginine detection device and detection method |
| CN114850487A (en) * | 2022-03-19 | 2022-08-05 | 昆明理工大学 | Preparation method of suspension containing nano-copper/nano-zinc oxide composite particles |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106706586B (en) | 2019-03-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lu et al. | Smartphone-integrated multi-color ratiometric fluorescence portable optical device based on deep learning for visual monitoring of Cu2+ and thiram | |
| Doroodmand et al. | Synthesis of a novel nitrogen-doped carbon dot by microwave-assisted carbonization method and its applications as selective probes for optical ph (acidity) sensing in aqueous/nonaqueous media, determination of nitrate/nitrite, and optical recognition of nox gas | |
| CN104034683B (en) | Method for measuring chlorine content of vanadium battery electrolyte solution | |
| CN104165915B (en) | A kind of for detection of silver-colored biology sensor and its preparation method and application | |
| CN106706586A (en) | Copper ion fluorescence detection test paper based on nano zinc oxide as well as preparation method and detection method thereof | |
| CN113406170B (en) | Ni (OH) for non-enzymatic glucose detection 2 Nanosheet sensor and preparation method and application thereof | |
| Wu et al. | Facile fabrication of Ag2 (bdc)@ Ag nano-composites with strong green emission and their response to sulfide anion in aqueous medium | |
| Ngo et al. | Highly sensitive smartphone-integrated colorimetric glucose sensor based on MnFe2O4–graphitic carbon nitride hybrid nanostructure | |
| CN108152263A (en) | A kind of method that ferro element is quickly detected based on carbon quantum dot fluorescence method | |
| CN114518358B (en) | Three-color sensing probe for detecting pesticide residues, preparation method thereof, application and visual intelligent monitoring device and method for deep learning | |
| Lu et al. | Deep learning-assisted smartphone-based portable and visual ratiometric fluorescence device integrated intelligent gel label for agro-food freshness detection | |
| He et al. | In situ growth of nanoflake and nanoflower-like Ni hydrated hydroxide on the surface of Ni foam as a free-standing electrode for high-performance phosphate detection | |
| Zhang et al. | Highly sensitive electrochemical sensing platform: Carbon cloth enhanced performance of Co 3 O 4/rGO nanocomposite for detection of H 2 O 2 | |
| Feng et al. | Artificial intelligence-assisted colorimetry for urine glucose detection towards enhanced sensitivity, accuracy, resolution, and anti-illuminating capability | |
| CN110243791B (en) | Method for detecting chloride ions in sweat through visual sensing | |
| Li et al. | A concise and mild green synthetic route of sodium chromate through ferrochrome electrochemical oxidation and in-situ purification | |
| Balamurugan et al. | Co-precipitation synthesis and characterization of rare-earth pyrochlore Gadolinium stannate; A novel electrocatalyst for the determination of furazolidone in water samples | |
| Lu et al. | Dual-channel MIRECL portable devices with impedance effect coupled smartphone and machine learning system for tyramine identification and quantification | |
| Peng et al. | Optimizing the chlorine evolution reaction performance of Co (OH) 2 catalyst for enhanced antifouling ability | |
| CN103217416B (en) | Detection composition, method and kit for detection of bivalent mercury ions | |
| CN106084188B (en) | A kind of preparation method of the porous organic ionic polymer elasticity of imidazole radicals | |
| Qu et al. | Selective and smart dual-channel colorimetric sulfur ion sensing readout platform | |
| Rezaei et al. | A selective modified bentonite–porphyrin carbon paste electrode for determination of Mn (II) by using anodic stripping voltammetry | |
| CN103084073B (en) | Porous membrane composed of cellulose doped with 1,4-dihydroxy anthraquinone and bivalent copper ion and preparation method and application thereof | |
| CN104569087A (en) | DNA/cuprous oxide composite material, biosensor, preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190308 Termination date: 20220109 |