CN106645116B - Biological rapid detection method based on smart phone two-dimensional code style - Google Patents
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Abstract
Biological rapid detection method based on smart phone two-dimensional code style, the rapid detection method are to prepare detection reaction vessels, the reaction figure be it is identical by size, color is different, the module that can be freely disassembled, assemble is spliced；Detection reagent is added in detection reaction vessels, chromogenic reaction occurs, the result after reaction is scanned using mobile phone camera, program is detected by smart phone and handles and show the testing result after reaction, realizes quickly detection.The advantages that the present invention is based on the quick detections that smart phone realizes biological respinse, have broad spectrum activity, easy to operate, at low cost, is applicable in the quick detection of various biologies on site.
Description
Technical field
The present invention relates to a kind of biological rapid detection methods, and it is automatic specifically to have write a kind of collection using Java language
It encodes, decode, being detected on integrated software, the detection reaction vessels printed using 3D printer, the molecule based on colorimetric method is known
Not, according to testing requirements, two-dimensional bar needed for setting generates experiment ties reaction, it can be achieved that the reaction of any biological color development
Result after beam is scanned with designed mobile phone A pp, obtains testing result, and then is the colour developing realized to the quantitative detection of sample
Reaction.
Background technique
In the 21 century that economic globalization, information networking, production internationalization are with rapid changepl. never-ending changes and improvements, new and high technology is not only to constitute
The key element of overall national strength, and the every field in the national economic development has been permeated, as information technology important composition portion
Point two-dimensional bar code this new and high technology, be under the premise of bar code is unable to satisfy modern information industry technology growth requirement
It generates.It solves the problems, such as that perplexing people always with bar code is described " article ", makes bar code veritably become letter
The effective tool of breath storage and identification.Two-dimensional bar code has information capacity big relative to bar code, and high reliablity can indicate to scheme
The advantages that kinds of words information such as picture, Chinese character, security and antiforge purpose is strong.Two-dimensional bar code be mainly characterized by two-dimensional bar code horizontal and
Vertical both direction indicates data information.According to its generating principle and planform, capable row's formula two-dimensional bar code can be classified as
And matrix two-dimensional barcode.Row row's formula two-dimensional bar code is on the basis of bar code, after being truncated by two rows or multirow height
Bar code accumulation, information expression is realized on the basis of increasing row identification, the characteristics such as error correcting.Matrix type two-dimension
Bar code is in planform with the feature of matrix.It is based on computer image processing technology, in matrix respective element
It on position, is indicated binary " 1 " with the appearance of square point, dot etc., binary " 0 " does not occur indicating in point, passes through point
Different permutation and combination indicate data information.Jiang et al. (Y. Zhang, L. B. Qiao, Y. K. Ren, X. W.
Wang, M. Gao, Y. F. Tang, J. Z. J. Xi, T-Y Fu and X. Y. Jiang,
Biomicrofludics, 2013, 7, 034110, Two dimensional barcode-inspired automatic
Analysis for arrayed microfluidic immunoassays) by Data Matrix and QR in two-dimensional bar code
Code has the excitation of automatic positioning function, devises the microchannel of two-dimensional structure, the design of the microchannel is by asymmetric
Red stripes are surrounded to determine detection zone, are then scanned with hand-held scanning means to the result after reaction, last benefit
Result is analyzed with computer software, high-throughput detection is realized, achievees the purpose that quantitative detection.This method operation quickly letter
It just, is not two dimension truly there is no coding rule is incorporated just with the concept of two dimensional code automatic positioning function
Code.Meanwhile the seminar (Y. Zhang, J. Sun, Y. Zou, W. W. Chen, W. Zhang, J. Z. J. Xi,
and X. Y. Jiang, Anal. Chem. 2015, 87, 900-906, Barcode Microchips for
Biomolecular Assays) structure and its coding rule that utilize one-dimensional bar code, according to requirement of experiment by bar code
Each " item " and " sky " is designed to microchannel, completes the detection of human body inhibition of HIV, and the microchannel can be used for examining simultaneously
Multiple pathogens are surveyed, it is after finally being reacted with hand held scanner scanning as a result, determining the property of sample in turn.This method has
Consume the advantages that agent is few, and channel is novel in design, and detection flux is high.
103272656 A of Publication No. CN discloses a kind of " Bar code micro fluidic chip and purposes ", which is related to one
Kind micro-fluidic chip, micro-fluidic chip includes chip unit, and the chip unit includes example reaction channel, in the sample
When the chromogenic reaction of sample occurring in reaction channel with the chromogenic reaction that sample does not occur, the bar code figure after being formed by reaction
It is different characters that case can be distinguished by bar code reader, so that it is determined that the property of sample.
In order to reach a kind of more convenience-for-people detection architecture and smart phone become increasingly popular not only brought it is huge
Big convenience, while also having attracted the extensive concern of numerous researchers at home and abroad.Due to the powerful image procossing of smart phone
Function and open application development environment, many researchers are applied in biological detection.With bar code
Universal, many researchers pass through research observation, it was found that the structure feature of bar code, and using this structure feature as life
Analyte detection channel.As Li et al. people (J. Guo, J. X. H. Wong, C. E. Cui, X. C. Li and H-Z. Yu,
A smartphone-readable barcode assay for the detection and quantitation of
Pesticide residues, Analyst, 2015,140,5518-5525) utilize 39 yards of "+", "-" structure in bar code
Particularity, 39 yards of homochromy "+" of the designed channel PDMS and printing are formed into " bio-barcode ", so that the biology item
Shape code can be read by cell phone application, achieve the purpose that a qualitative detection.And then in order to realize further detection, Yu etc.
People (J. X. H. Wong, X. C. Li, F. S. F. Liu and H-Z. Yu, Direct Reading of Bona
Fide Barcode Assays for Diagnostics with Smartphone Apps, Scientific Reports
2015,5,11727) mobile phone A pp is utilized, " the biology in conjunction with composed by 39 yards of "+" in bar code, the particularity of "-" structure
Bar code " realizes the quantitative detection of hCG.This method does not need expensive instrument not only, but also be applicable on site quick examines
It is disconnected.
Therefore, the coding rule of present invention combination two-dimensional bar, the module of 3D printer printing are designed to detection reaction
Vessel detect program using smart phone, and self-editing one kind can collect coding, decode, be detected on integrated mobile phone A pp, realize life
Change rapid reaction testing goal.
Summary of the invention
The invention solves concrete technical problems be that existing biochemical reaction detection method usually requires expensive profession
Instrument；And it is based on smart phone detection system, using two dimensional code pattern as sense channel and incorporate the biochemistry of coding rule instead
Detection method is answered not to be suggested at present.The module that the purpose of the present invention prints 3D printer is utilized as detection reaction vessels
The characteristics of it can be freely disassembled, assemble realizes quick, the portable detection to biochemical reaction in conjunction with smart phone detection program
Method establishes a kind of biological rapid detection method based on smart phone two-dimensional code style.
The coding rule part of two dimensional code is dissolved among the design of chip by the present invention, and write collection coding, decoding,
It is detected on integrated mobile phone A pp.
Measure adopted by the present invention is the biological rapid detection method based on smart phone two-dimensional code style, the method
It is using the module of 3D printer printing as detection reaction vessels, the characteristics of can arbitrarily dismantling, assemble using it, in conjunction with oneself
The mobile phone A pp program write, automatically generates required pattern in 2 D code, is assembled, spliced according to the pattern of generation, is made
Detection reaction vessels needed for reaction are scanned the result after chromogenic reaction then by mobile phone decoding program, according to sweeping
Character after retouching is compared with blank pattern, determines the property of sample, and the picture after interception reaction analyzes picture,
Achieve the purpose that quantitative detection.
The detection reaction vessels that the present invention designs include that sample chromogenic reaction occurs and does not occur two kinds of sample chromogenic reaction
Situation, the pattern generated after the reaction are enough to read result by mobile phone A pp.
Colour contrast rate after sample generation chromogenic reaction is added in detection reaction vessels mentioned in the present invention is enough
Greatly.
The specific method is as follows for its described speed survey.
Biological rapid detection method based on smart phone two-dimensional code style, the rapid detection method are by following step
Suddenly it carries out:
Preparation for biological respinse detection vessel；
For reagent to be detected to be added in the detection reaction vessels, for realizing the colour developing of sample amounts detection
Reaction；
Smart phone for biological respinse detection detects program, shows testing result；
The detection reaction vessels are to be printed as material by 3D printer by 3DMax software design using photosensitive resin
Detect reaction vessels；
The detection reaction vessels are to be freely disassembled, assembled according to requirement of experiment.
Further, the rapid detection method follows these steps to carry out:
(1) it is used for the preparation of biological detection reaction vessels, is by the identical module composition of several sizes, outside each module
It is 8mm, Gao Shi 3mm that length, which is 8mm, width, and interior length is 7mm, and wide is 7mm, 1 ㎜ of Gao Shi, moves freely, assembled constitutes a two dimensional code
The detection pattern of pattern；
2) for reagent to be detected to be added in the biological detection reaction vessels, the aobvious of sample amounts detection is realized
Colour response is that sample is equipped in the biological detection reaction vessels, in example reaction vessel occur sample chromogenic reaction with
Under the chromogenic reaction two states that sample does not occur, the color in example reaction channel is able to read phase by mobile phone reading device
The data result answered passes through the figure compared with results of comparison, according to the property of acquired results and then determining sample, after interception reaction
Piece information analyzes the picture of acquisition, shows testing result；
(3) smart phone for biological respinse detection detects program, shows testing result, is compiled by Java language
It writes and operates on Android phone platform.
Further, additional technical feature is as follows.
Example reaction in the detection reaction vessels is same sample reaction or different example reactions, same detection reaction
Example reaction detection reagent in vessel is same reaction detection reagent or differential responses detection reagent.
The sample detection reaction vessels are that same sample detects reaction vessels or different sample detection reaction vessels, same
Example reaction in example reaction detection vessel is same sample reaction or different example reactions.
The module be made of one group of structure, including position sensing area, position sensing marker space, positioning area, correction zone,
Version information area, format information area and data field.
The module of the two dimensional code is detection reaction vessels region, remaining module is the non-reaction vessels region of detection.
The detection reaction vessels region is reacted in data field, and not reacting in data field is non-detection reaction
Vessel region.
The detection reaction vessels quantity of the two dimensional code is to determine the number for detecting reaction vessels according to experiment testing requirements
Amount.
The module is made of according to detection demand the different little module of color each unit.
The speed detection method is benefit under conditions of making sample be enough to occur chromogenic reaction in test sample reaction vessels
Channel is distinguished with mobile phone recognition App, the property of sample is determined according to the character of recognition, after mobile phone A pp interception reaction
Picture is obtained, picture is analyzed, realizes rapid quantitative detection.
Realize the above-mentioned biological rapid detection method based on smart phone two-dimensional code style, the fast determining method is to prepare life
Change reaction detection vessel, detection reaction vessels be it is identical by size, it is any dismantle, the module of assembling is spliced；In device
Detection reagent is added in ware, chromogenic reaction occurs, the result after reaction is scanned using mobile phone camera, passes through intelligent hand
Machine testing program handles and shows the colour developing result after chromogenic reaction.The present invention is based on smart phones to realize the quick of biochemical reaction
Detection, have it is easy to carry, it is easy to operate, it is low in cost, it is quick the advantages that, be suitable for field quick detection.
Compared with prior art, this fast determining method embodies a concentrated reflection of following several points:
(1) generation of two-dimensional bar can be determined, and then determine the quantity of detection vessel according to the demand of detection.
(2) detection reaction vessels can arbitrarily be dismantled according to requirement of experiment, be assembled.
(3) reagent dosage is few, greatly reduces testing cost.
(4) using smart phone detection program recognition chromogenic reaction after colour developing as a result, really realize a step quickly detect,
Substantially reduce detection time.
(5) entire detection process only needs a smart phone, i.e., experiment institute is arbitrarily generated with self-editing mobile phone A pp
The two-dimensional bar figure needed, the module printed in conjunction with 3D printer is spliced according to two-dimension code pattern generated to be assembled, in number
The sample of requirement of experiment is added according to the place of the determination in area, chromogenic reaction occurs, recycles mobile phone A pp to the knot after chromogenic reaction
Fruit is scanned, and compares with the original two dimensional code pattern of above-mentioned assembly, test sample property, the picture letter after interception reaction
Breath, analyzes the picture of the acquisition, shows testing result.It is wide that above-mentioned technical requirements popularize range.
Detailed description of the invention
Fig. 1 is the detection reaction vessels top view printed in the present invention with 3D printer.
Fig. 2 is the detection reaction vessels bottom view printed in the present invention with 3D printer.
Fig. 3 is to utilize the pattern in 2 D code of mobile phone coded program generation for 03.
Fig. 4 is the pattern in 2 D code after the module assembly using 3D printer printing.
Fig. 5 is the smart phone detection program runnable interface that the present invention designs.
Fig. 6 is smart phone decoding of the present invention interface.
Fig. 7 is smart phone decoding result of the present invention.
Fig. 8 is preview interface of the present invention.
Fig. 9 is interpretation of result interface of the present invention.
Specific embodiment
Below to detect in fruits and vegetables for parathion-methyl, as control for 03 pattern generated using two dimensional code, to this
The specific embodiment of invention is further described in detail.
Implement a kind of above-mentioned provided smart phone two-dimensional code biology rapid detection method of the present invention, the quick detection side
Specific step is as follows for method:
(1) 03 character is inputted using Encode, generates the pattern of two dimensional code pattern.
(2) preparation of microchannel: utilizing 3D laser printer, using bright resin as material, by polishing, is made outer a length of
8mm, width are 8 ㎜, and a height of 3mm, the example reaction head of district is 7mm, and width is 7 ㎜, and a height of 1mm, the square detection that base thickness is 1mm is instead
Pattern in 2 D code needed for answering vessel to be spliced into experiment, is made biological respinse vessel.
(3) parathion-methyl standard curve making: configuring the PBS buffer solution (pH=8) of 510mL, and taking 1mL concentration is 100 μ
The parathion-methyl titer of g/mL is diluted to 10 μ g/mL with ultrapure water (buffer), is placed in volumetric flask and keeps away in 4 DEG C of refrigerator
Light saves, spare.It is dissolved in PBS buffer solution using the parathion-methyl titer of 10 μ g/mL as mother liquor, is configured to concentration difference
Be placed in brown centrifuge tube for the 200 μ L of parathion-methyl solution of 0,0.5,2,4,6 μ g/mL, be separately added into 10 μ L color developing agents and
The mixing of 10 μ L enzyme solutions shakes up, and is placed in 37 DEG C of insulating box and cultivates 30min.10 μ L substrates are added in above-mentioned centrifuge tube, timing,
Mixing shakes up.It is each triplicate.Once take the parathion-methyl solution of the above-mentioned various concentration of the configuration of 49 μ L that step is added respectively
In microchannel described in rapid 1, is scanned after 10min and analyze result.
(4) in fruits and vegetables parathion-methyl detection: for detecting the parathion-methyl in apple.It will be from local supermarket
The apples peeling bought back.It weighs 5g apple epidermis to be dissolved in the phosphate buffer of 10mL, quickly shakes up 5min, extract above-mentioned molten
Liquid goes 200 μ L supernatants to be detected as the residual liquid addition centrifuge tube to be checked of agriculture in being centrifuged 3min in centrifuge.
(5) smart phone detects program reading process: clicking icon on mobile phone screen and enters main interface, main interface includes
Four buttons, respectively New Test, Decode, Encode, Quit.It clicks Decode button and enters mobile phone decoding program, show
It is after showing chromogenic reaction as a result, click return push-button enter main interface.It clicks New Test button and enters mobile phone camera preview circle
Face can show a pre-set screen interception square alignment box at this time in preview interface, by the reaction item after colour developing
After band is to dead square bar frame, Capture button is clicked, program can intercept the picture in square-shaped frame automatically and calculate in RGB
B value be saved in mobile phone, then click Analyses button, program will automatically calculate out the B value in frame, and according to methyl pair
The concentration of sulphur phosphorus titer and corresponding B value ratio fit Calibration equation, in conjunction with this Calibration equation B value meter corresponding with liquid to be checked
The concentration value in sample to be tested in parathion-methyl sample is calculated, result is finally will test and directly displays onto interface.
Claims (2)
1. the biological rapid detection method based on smart phone two-dimensional code style, the rapid detection method are to follow these steps
It carries out:
Preparation for biological respinse detection vessel；
For reagent to be detected to be added in biological respinse detection vessel, for realizing the colour developing of sample amounts detection
Reaction；
Smart phone for biological respinse detection detects program, shows testing result；
The biological respinse detection vessel are to be printed as material by 3D printer by 3DMax software design using photosensitive resin
Biological respinse detects vessel；
The biological respinse detection vessel are to be freely disassembled, assembled according to requirement of experiment；
The specific method that the biology quickly detects follows these steps to carry out:
(1) preparation for biological respinse detection vessel, is by the identical module composition of several sizes, each module foreign minister is
8mm, width are 8mm, Gao Shi 3mm, and interior length is 7mm, and wide is 7mm, 1 ㎜ of Gao Shi, are moved freely, one two dimensional code pattern of assembled composition
Detection pattern；
(2) for reagent to be detected to be added in biological respinse detection vessel, the colour developing of sample amounts detection is realized
Reaction is that sample is equipped in biological respinse detection vessel, and sample biological respinse detects the colour developing that sample occurs in vessel
Under reaction and the chromogenic reaction two states that sample does not occur, the color in example reaction channel is able to by mobile phone reading device
Corresponding data result is read, by the way that compared with results of comparison, according to the property of acquired results and then determining sample, interception is reacted
Pictorial information afterwards analyzes the picture of acquisition, shows testing result；
(3) smart phone for biological respinse detection vessel detects program, shows testing result, is write simultaneously by Java language
It operates on Android phone platform；
The biological respinse detection vessel are that same sample biological respinse detects vessel or different sample biological respinses detect vessel；
The example reaction of the biological respinse detection vessel is same sample reaction or different example reactions, the sample in same reaction vessels
Product reaction detection reagent is same reaction detection reagent or differential responses detection reagent；
The module is made of according to detection demand the different little module of color each unit, including position sensing area, position
Set detection marker space, positioning area, correction zone, version information area, format information area and data field；
The biological respinse detection vessel quantity of the two dimensional code is determined according to testing requirements；The module section of the two dimensional code
It is conversion zone, remaining module is non-conversion zone.
2. biology rapid detection method as described in claim 1, the rapid detection method is to keep sample anti-in sample biology
Under conditions of vessel mesopodium should be detected so that chromogenic reaction occurs, the pattern after chromogenic reaction is distinguished using mobile phone A pp, root
The property that sample is determined according to the character of recognition is analyzed picture, is realized quick with picture is obtained after mobile phone A pp interception reaction
Quantitative detection.
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| CN107356737B (en) * | 2017-07-03 | 2019-07-23 | 华南理工大学 | A kind of production method of Activity determination two dimensional code |
| CN107727651A (en) * | 2017-08-29 | 2018-02-23 | 太原理工大学 | The quick high-precision fixed amount detecting devices of pH in scene and method based on smart machine |
| CN110927145A (en) * | 2018-09-20 | 2020-03-27 | 安徽合大环境检测有限公司 | Experimental preprocessing color image capturing, comparing and analyzing system |
| US20220020481A1 (en) | 2020-07-20 | 2022-01-20 | Abbott Laboratories | Digital pass verification systems and methods |
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