CN106630190A - COD (chemical oxygen demand)-degrading fungicide agent and preparation method thereof - Google Patents
COD (chemical oxygen demand)-degrading fungicide agent and preparation method thereof Download PDFInfo
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Abstract
The invention provides a COD (chemical oxygen demand)-degrading fungicide agent and a preparation method thereof. The COD-degrading fungicide agent comprises compound fungicide liquid, a protective agent and a carrier, wherein the compound fungicide liquid comprises Aspergillus calidoustus liquid, trichoderma citrinoviride liquid, aspergillus niger liquid and rhizopus stolonifer liquid; the protective agent comprises skim milk powder, trehalose, calcium carbonate and glycerin. The preparation method comprises the following steps of (1) mixing the Aspergillus calidoustus liquid, the trichoderma citrinoviride liquid, the aspergillus niger liquid and the rhizopus stolonifer liquid into the compound fungicide liquid, adding the protective agent, and uniformly mixing; (2) crushing the carrier and treating by a 60 to 100-mesh sieve; (3) adding the crushed carrier into the compound fungicide liquid, uniformly mixing, pre-freezing, and drying, so as to obtain the fungicide agent. The fungicide agent has the advantages that the fungicide agent is used as a degradable microorganism, the strain is stable, the synergistic action is obvious, and the COD in sewage is favorably degraded.
Description
Technical field
The invention belongs to water treatment micro-organism formulation art, and in particular to one kind degraded COD fungal inoculants and its preparation side
Method.
Background technology
At present, incomparable effect is played in industry, cultivation and the process of sanitary wastewater in terms of environmental protection, but
Sewage treatment area the inside yet suffers from many problems, and such as trade effluent is unstable in water quality treatment effect, and runs
Relatively costly, the water after purification is needed through being passed through the disinfections such as chlorine, causes originally to be micro- inside carrying
There is mortality in biology, so as to allow water body to lose the ability of the auto purification of script, the chemical composition in sewage-treating agent is present
Higher phenomenon, easily causes secondary pollution etc., the COD and ammonia nitrogen superelevation of aquaculture wastewater to water body.
Than chemical method, biological method processes waste water and has the following advantages：(1) every kind of being both for property of chemical article is very strong
Product, failure is possible to when other chemical substances are run into, and removal of the biologic product to pollutant has light spectrality；(2) change
Learning product can temporarily eliminate some harmful substances and cover stink, and lacking can not prevent the generation of harmful substance；(3) useization
After learning product, residual is had in water body, secondary pollution may be caused.Natural microbial contained by biologic product, without pathogenic bacteria and
Pathogen, these microorganisms under the catalytic action of enzyme, with the organic nutrient substance in sewage as food, when sewage is purified
Afterwards, these microbes are gradually decreased with the reduction of pollutant, until withering away；(4) nontoxic, non-corrosiveness is easy to use, base
Originally equipment or engineering need not be added, fund input is saved.
Trade effluent composition is more complicated, and particularly substantial amounts of artificial-synthetic compound enters environment, and this kind of material is main
It is alkanes, olefines, alicyclic hydrocarbon type, arene, artificial synthesized organic matter, especially macromolecular, difficult degradation, poisonous has
Evil material such as phenol, chlorophenol, cresols, nitrophenols etc., arene, cyanogen class, amine and ammonia nitrogen etc. giving up with plant emissions
Water or solid waste enter environment, due to the complexity and the strange property of biology of these materials this body structures, at short notice can not be by
Microorganism decomposes utilization.The microorganism of traditional method of wastewater treatment activated sludge culture can not effectively to these
Pollutant is removed, and these materials are accumulated in the environment for a long time, and to the ecological environment that we depend on for existence very big pollution is caused.
There is problems with existing microbial degradation COD inorganic agents：
1) complex microorganism preparations on market in COD degradation microbial inoculum are generally based on bacterium, fungi composite bacteria agent application
Less, the degradation of fungi is not fully excavated；2) bacterial classification instability problem, has a strong impact on the production of microorganism formulation and answers
With bacterial classification is purified and tames unstable；3) stability of complex microorganism preparations is not strong, easily affected by environment, group structure or
Because environmental change makes certain micro-organisms dead or because the intervention of new microorganism causes the community superiority quilt of complex microbial inoculum
Change；4) microbial profile is not uniform enough in complex micro organism fungicide, and same species of microorganism distribution is more concentrated, and compartmentalization is presented and shows
As；5) mostly the research of correlation and effect is not deep enough between bacterium in mixed culture, causes the product developed for having
The bacterial strain screening of synergy relation and combination or random process, lack effective theoretical direction, and for
The co-culture system of Jing applications can not effectively coordinate the relation between bacterium so as to up to optimal Ecological level, play maximum effect
Should.
The content of the invention
To solve the problems, such as existing complex microorganism preparations, the present invention proposes a kind of degraded COD fungal inoculants, the bacterium
Using fungi as degrading microorganism, not only bacterial classification is stablized, and it is obvious to act synergistically, and is conducive to the COD degraded in sewage for agent.
The technical scheme is that what is be achieved in that：
One kind degraded COD fungal inoculants, are made up of, the composite fungi bacterium composite fungi bacterium solution, protective agent and carrier
Liquid includes Aspergillus calidoustus bacterium solutions, lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and rhizopus stolonifer bacterium solution,
The protective agent includes skimmed milk power, trehalose, calcium carbonate and glycerine.
Further, the Aspergillus calidoustus bacterium solutions, lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and
The volume ratio of rhizopus stolonifer bacterium solution is 15～30：10～25:20～35：10～30.
Further, the spore count in the Aspergillus calidoustus bacterium solutions reaches 1.5～5.5 × 105cfu/
Ml, the spore count of the lemon green trichoderma bacterium solution reaches 1.5～5.5 × 105Cfu/ml, the spore count of the aspergillus niger bacterium solution reaches
To 1.5～5.5 × 105Cfu/ml, the spore count of the rhizopus stolonifer bacterium solution reaches 1.5～5.5 × 105cfu/ml。
Further, the quality of the skimmed milk power and the volume ratio of the composite fungi bacterium solution are 5～15g:100ml, institute
The volume ratio of the quality and the composite fungi bacterium solution of stating trehalose is 1～10g:100ml, the quality of the calcium carbonate with it is described
1～the 10g of volume ratio of composite fungi bacterium solution:100ml, the volume ratio 0.5 of the quality of the glycerine and the composite fungi bacterium solution
～5.5g:100ml.
Further, the carrier is wheat bran or rice bran.
Further, volume ratio 1g of the quality of the carrier and the composite fungi bacterium solution：0.5～3ml.
It is a further object to provide a kind of preparation method of degraded COD fungal inoculants, comprises the following steps：
1) by Aspergillus calidoustus bacterium solutions, lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and rhizopus stolonifer
Bacterium solution is mixed to get composite fungi bacterium solution, is subsequently adding protective agent and is well mixed；
2) carrier is carried out crushing 60～100 mesh sieve subprocessings；
3) carrier that pulverization process is crossed is added in composite fungi bacterium solution again and is well mixed, then pre-freeze is processed, then is entered
Row drying, you can.
Preferably, in some embodiments of the invention, Aspergillus calidoustus cultural methods are：
(1) primary inclined plane culture：Culture medium prescription：Potato 200g, glucose 20g, magnesium sulfate 1.5g peptone 2g, phosphorus
Acid dihydride potassium 1.5g, dipotassium hydrogen phosphate 1.5g, agar 20g, add water to 1000ml, pH7.0～8.0.By Aspergillus
Calidoustus is inoculated on the inclined-plane of above-mentioned culture medium, cultivates 2～4d, then prepares bacteria suspension, and 10 are contained in bacterium solution5cfu/
ml；
(2) secondary seed culture：Aspergillus calidoustus are inoculated with according to 1% inoculum concentration to train to secondary seed
In foster base, concussion and cultivate (rotating speed 180rpm), 23～30 DEG C of 2～4d of culture, dilution spread is counted.Secondary seed medium：Sulphur
Sour magnesium 0.35g, sodium chloride 0.45g, potassium dihydrogen phosphate 1.15g, urea 5.65g, glucose 5.65g, corn flour 6g, add water to
1L, adjusts pH to 2.5 or so.
The cultural method of lemon green trichoderma, aspergillus niger and rhizopus stolonifer and Aspergillus calidoustus are cultivated
Method is essentially identical, and difference is that bacterial classification is different.
Aspergillus calidoustus bacterium solutions are that Aspergillus calidoustus thalline are seeded into NaCl
Solution.
Beneficial effects of the present invention：
1st, strain source is convenient, and institute is learned to do using bacterial screening is from sewage treatment plant and is purified by molecular biosciences
Duan Jinhang is identified, and acclimation is carried out in sewage, using various culture collection process such as ultra low temperature freezer and liquid nitrogen；
2nd, the bacterial classification for being adopted be easy to culture, simple production process, obtained degraded COD fungal inoculants be easy to preserve and
Transport；
3rd, the microorganism that product of the present invention is selected is through antagonism/synergy experiment, relation between effective coordination bacterium so as to reach
To optimal Ecological level, maximum ecological effect has been given play to；
4th, the larger molecular organicses in efficient degradation sewage, degradation capability is strong, effect stability when processing various biochemical indicators,
It is regular strong；
5th, COD fungal inoculants stability is strong, is difficult to be affected by environmental change, effectively reaches the finger of complex microbial inoculum
Mark.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is that the microbial inoculum of embodiment 1 tests water outlet COD contrast curves；
Fig. 2 is that the microbial inoculum of embodiment 1 tests water outlet SS contrast curves.
Specific embodiment
Embodiment 1
One kind degraded COD fungal inoculants, are made up of, composite fungi bacterium solution bag composite fungi bacterium solution, protective agent and carrier
Aspergillus calidoustus bacterium solutions, lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and rhizopus stolonifer bacterium solution are included, is protected
Agent includes skimmed milk power, trehalose, calcium carbonate and glycerine, and carrier is wheat bran.Aspergillus calidoustus bacterium solutions, lemon
The volume ratio of lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and rhizopus stolonifer bacterium solution is 15：25:20：30.
Wherein, the spore count in Aspergillus calidoustus bacterium solutions reaches 4.5 × 105Cfu/ml, the green wood of lemon
The spore count of mould liquid reaches 4.5 × 105Cfu/ml, the spore count of aspergillus niger bacterium solution reaches 4.5 × 105Cfu/ml, rhizopus stolonifer
The spore count of bacterium solution reaches 4.5 × 105cfu/ml。
Preparation method, comprises the following steps：
1) by Aspergillus calidoustus bacterium solutions, lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and rhizopus stolonifer
Bacterium solution is mixed to get composite fungi bacterium solution, is subsequently adding protective agent and is well mixed；Wherein, the quality and composite fungi of skimmed milk power
The volume ratio of bacterium solution is 10g:100ml, the quality of trehalose is 8g with the volume ratio of composite fungi bacterium solution:100ml, calcium carbonate
Volume ratio 8g of quality and composite fungi bacterium solution:100ml, the quality of glycerine and volume ratio 4g of composite fungi bacterium solution:100ml；
2) wheat bran is carried out crushing 80 mesh sieve subprocessings；
3) carrier that pulverization process is crossed is added in composite fungi bacterium solution again and is well mixed, be then placed in -20 DEG C of pre-freezes
8h, is then dried 10h under the conditions of -45 DEG C of 10～20Pa pressure, you can.
Embodiment 2
One kind degraded COD fungal inoculants, are made up of, composite fungi bacterium solution bag composite fungi bacterium solution, protective agent and carrier
Aspergillus calidoustus bacterium solutions, lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and rhizopus stolonifer bacterium solution are included, is protected
Agent includes skimmed milk power, trehalose, calcium carbonate and glycerine, and carrier is wheat bran.Aspergillus calidoustus bacterium solutions, lemon
The volume ratio of lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and rhizopus stolonifer bacterium solution is 30：20:30：20.
Wherein, the spore count in Aspergillus calidoustus bacterium solutions reaches 1.5 × 105Cfu/ml, the green wood of lemon
The spore count of mould liquid reaches 5.5 × 105Cfu/ml, the spore count of aspergillus niger bacterium solution reaches 1.5 × 105Cfu/ml, rhizopus stolonifer
The spore count of bacterium solution reaches 5.5 × 105cfu/ml。
Preparation method, comprises the following steps：
1) by Aspergillus calidoustus bacterium solutions, lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and rhizopus stolonifer
Bacterium solution is mixed to get composite fungi bacterium solution, is subsequently adding protective agent and is well mixed；Wherein, the quality and composite fungi of skimmed milk power
The volume ratio of bacterium solution is 5g:100ml, the quality of trehalose is 10g with the volume ratio of composite fungi bacterium solution:100ml, calcium carbonate
Volume ratio 2g of quality and composite fungi bacterium solution:100ml, the quality of glycerine and volume ratio 0.5g of composite fungi bacterium solution:
100ml；
2) rice bran is carried out crushing 60 mesh sieve subprocessings；
3) carrier that pulverization process is crossed is added in composite fungi bacterium solution again and is well mixed, be then placed in -20 DEG C of pre-freezes
8h, is then dried 10h under the conditions of -45 DEG C of 10～20Pa pressure, you can.
Embodiment 3
One kind degraded COD fungal inoculants, are made up of, composite fungi bacterium solution bag composite fungi bacterium solution, protective agent and carrier
Aspergillus calidoustus bacterium solutions, lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and rhizopus stolonifer bacterium solution are included, is protected
Agent includes skimmed milk power, trehalose, calcium carbonate and glycerine, and carrier is wheat bran.Aspergillus calidoustus bacterium solutions, lemon
The volume ratio of lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and rhizopus stolonifer bacterium solution is 15：10:35：10.
Wherein, the spore count in Aspergillus calidoustus bacterium solutions reaches 5.5 × 105Cfu/ml, the green wood of lemon
The spore count of mould liquid reaches 1.5 × 105Cfu/ml, the spore count of aspergillus niger bacterium solution reaches 5.5 × 105Cfu/ml, rhizopus stolonifer
The spore count of bacterium solution reaches 1.5 × 105cfu/ml。
Preparation method, comprises the following steps：
1) by Aspergillus calidoustus bacterium solutions, lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and rhizopus stolonifer
Bacterium solution is mixed to get composite fungi bacterium solution, is subsequently adding protective agent and is well mixed；Wherein, the quality and composite fungi of skimmed milk power
The volume ratio of bacterium solution is 15g:100ml, the quality of trehalose is 1g with the volume ratio of composite fungi bacterium solution:100ml, calcium carbonate
Volume ratio 6g of quality and composite fungi bacterium solution:100ml, the quality of glycerine and volume ratio 5.5g of composite fungi bacterium solution:
100ml；
2) wheat bran is carried out crushing 100 mesh sieve subprocessings；
3) carrier that pulverization process is crossed is added in composite fungi bacterium solution again and is well mixed, be then placed in -20 DEG C of pre-freezes
8h, is then dried 10h under the conditions of -45 DEG C of 10～20Pa pressure, you can.
Embodiment 4
The degraded COD fungal inoculants prepared using embodiment 1 carry out degrading waste water COD experiment as Typical Representative.
1) raw wastewater (the preliminary sedimentation tank water outlet of the existing handling process in certain chemical plant, and active dirt is taken from aeration tank is taken
Mud is used to process test), and detected that both approaches are by two kinds of different examination criterias：①CODcr:Breathe out in the U.S.
Uncommon potassium dichromate method colorimetric method.In strongly acidic solution, using potassium dichromate oxidation Organic substance in water, colorimetric detection is then used
Learn oxygen demand.②SS:U.S.'s Hash detection method.
The data for measuring raw wastewater water quality are following (March 1):
| Title | Aerobic water inlet |
| COD(mg/L) | 560 |
| SS(mg/L) | 195 |
2) tested in sewage reactor using following methods：
1. the microbial inoculum of embodiment 1：Raw wastewater+activated sludge+urea+microbial inoculum of potassium dihydrogen phosphate+embodiment 1
2. blank experiment:Raw wastewater+activated sludge+urea+potassium dihydrogen phosphate
3) match can microbial inoculum addition be only 0.05wt%-0.5wt%, and with this inoculum concentration activated sludge can be significantly improved
Activity, improves the degradation capability of COD, and improves the settling property of sludge.
4) detect to testing effluent COD concentration and SS concentration, detection data following (origination date is March 1)
As can be seen that not adding the blank control group of embodiment 1 due to effectively micro- in activated sludge from the data of table 1
Biomass is less, limited to the degradation capability of COD, and the larger molecular organicses in sewage can not effectively be degraded, and cause water outlet
COD and SS is apparently higher than the experimental group for adding the microbial inoculum of embodiment 1, therefore it may be concluded that the experiment of the addition microbial inoculum of embodiment 1
Group can effectively improve the degradation capability of COD in sewage.
Referring to Fig. 1 (being compared drafting by the concentration to processing COD of sewage to obtain), add the reality of the microbial inoculum of embodiment 1
It is 106.3mg/L to test the average COD concentration of group water outlet, and the average COD concentration of water outlet of blank control group is 137.2mg/L, and addition is real
The experimental group water outlet COD for applying the microbial inoculum of example 1 is significantly lower than blank group, illustrates that the microbial inoculum of embodiment 1 can significantly improve system degraded
The ability of COD in waste water.
Referring to Fig. 2 (being compared drafting to the SS concentration of experiment process water to obtain), it will be seen that with the addition of enforcement
The experimental group water outlet SS of the microbial inoculum of example 1 will be far below blank assay group, illustrate that adding the microbial inoculum of embodiment 1 can improve system activity dirt
The settleability of mud.
Presently preferred embodiments of the present invention is the foregoing is only, not to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.
Claims (7)
1. a kind of degraded COD fungal inoculants, it is characterised in that be made up of composite fungi bacterium solution, protective agent and carrier, it is described multiple
Closing fungi bacterium solution includes Aspergillus calidoustus bacterium solutions, lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and ramose root of crawling
Mould liquid, the protective agent includes skimmed milk power, trehalose, calcium carbonate and glycerine.
2. degraded COD fungal inoculants according to claim 1, it is characterised in that the Aspergillus
The volume ratio of calidoustus bacterium solutions, lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and rhizopus stolonifer bacterium solution is 15～30：10
～25:20～35：10～30.
3. degraded COD fungal inoculants according to claim 1 and 2, it is characterised in that the Aspergillus
Spore count in calidoustus bacterium solutions reaches 1.5～5.5 × 105Cfu/ml, the spore count of the lemon green trichoderma bacterium solution reaches
To 1.5～5.5 × 105Cfu/ml, the spore count of the aspergillus niger bacterium solution reaches 1.5～5.5 × 105Cfu/ml, the ramose root of crawling
The spore count of mould liquid reaches 1.5～5.5 × 105cfu/ml。
4. degraded COD fungal inoculants according to claim 1, it is characterised in that the quality of the skimmed milk power with it is described
The volume ratio of composite fungi bacterium solution is 5～15g:100ml, the volume ratio of the quality of the trehalose and the composite fungi bacterium solution
For 1～10g:100ml, the 1～10g of volume ratio of the quality of the calcium carbonate and the composite fungi bacterium solution:100ml is described sweet
The quality of oil and the 0.5～5.5g of volume ratio of the composite fungi bacterium solution:100ml.
5. degraded COD fungal inoculants according to claim 1, it is characterised in that the carrier is wheat bran or rice bran.
6. degraded COD fungal inoculants according to claim 1 and 2, it is characterised in that the quality of the carrier is multiple with described
Close volume ratio 1g of fungi bacterium solution：0.5～3ml.
7. a kind of preparation method of degraded COD fungal inoculants as claimed in claim 1, it is characterised in that comprise the following steps：
1) by Aspergillus calidoustus bacterium solutions, lemon green trichoderma bacterium solution, aspergillus niger bacterium solution and rhizopus stolonifer bacterium solution
Composite fungi bacterium solution is mixed to get, protective agent is subsequently adding and is well mixed；
2) carrier is carried out crushing 60～100 mesh sieve subprocessings；
3) carrier that pulverization process is crossed is added in composite fungi bacterium solution again and is well mixed, then pre-freeze is processed, then is done
It is dry, you can.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107400648A (en) * | 2017-08-29 | 2017-11-28 | 安徽瑞驰兰德生物科技有限公司 | A kind of complex microorganism combines microbial inoculum, its preparation method and application, a kind of comprehensive processing method of black-odor riverway |
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| CN103525870A (en) * | 2013-10-23 | 2014-01-22 | 嘉兴学院 | Microbial flocculant as well as preparation method and application thereof |
| CN103540549A (en) * | 2013-10-24 | 2014-01-29 | 哈尔滨工业大学 | Preparation method of compound microbial inoculum for degrading domestic sewage |
| CN104894033A (en) * | 2015-06-25 | 2015-09-09 | 湖北凌卓生物工程有限公司 | Compound microbial inoculant for degrading COD (chemical oxygen demand) and preparation method of compound microbial inoculant |
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| CN107400648A (en) * | 2017-08-29 | 2017-11-28 | 安徽瑞驰兰德生物科技有限公司 | A kind of complex microorganism combines microbial inoculum, its preparation method and application, a kind of comprehensive processing method of black-odor riverway |
| CN107400648B (en) * | 2017-08-29 | 2020-09-15 | 安徽瑞驰兰德生物科技有限公司 | Composite microbial inoculum, preparation method and application thereof, and comprehensive treatment method of black and odorous river channel |
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