CN106621824B - The disintegration agent of Biofilm contamination in a kind of alleviation membrane bioreactor - Google Patents
The disintegration agent of Biofilm contamination in a kind of alleviation membrane bioreactor Download PDFInfo
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- CN106621824B CN106621824B CN201710001417.3A CN201710001417A CN106621824B CN 106621824 B CN106621824 B CN 106621824B CN 201710001417 A CN201710001417 A CN 201710001417A CN 106621824 B CN106621824 B CN 106621824B
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- disintegration agent
- membrane bioreactor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
- B01D65/02—Membrane cleaning or sterilisation ; Membrane regeneration
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Abstract
The invention discloses a kind of disintegration agent of Biofilm contamination in alleviation membrane bioreactor, preparation method includes: to prepare the deoxyribonuclease I and D- tyrosine formic acid solution of different quality concentration respectively, above-mentioned solution is uniformly mixed, the disintegration agent activator Mn of certain mass concentration is added after pH value is adjusted2+.Using when Biofilm contamination disintegration agent added into membrane bioreactor backwashing water, dosage and backwashing water volume ratio are 1:100~150.Biofilm contamination disintegration agent significantly extends the membrane bioreactor slow stage fouling membrane time, and deoxyribonuclease I and D- tyrosine play decomposition to film surface biofilm formation key component, is used in combination with synergistic effect；Activator Mn2+There is activation to deoxyribonuclease I and D- tyrosine；The Biofilm contamination disintegration agent diffused in bulk solution can reduce activated sludge multiplication rate, reduce excess sludge discharge amount.
Description
Technical field
The invention belongs to water-treatment technology fields, specifically a kind of to delay Biofilm contamination disintegration agent in membrane bioreactor
Preparation and application method.
Background technique
Traditional activated sludge process is filtered into membrane bioreactor (MBR) technique combined with film, good with effluent quality,
The advantages that occupied area is small is concerned in sewage treatment and Treated sewage reusing field.However due to the formation of film surface biomembrane
And the cake layer then adhered to causes serious fouling membrane, constrains membrane biological reaction while significantly reducing membrane flux
The extensive extensive use of device technique.Early-stage study show 60% or more of membrane bioreactor operating cost directly with biology
Fouling membrane is related, thus how to control angle from membrane bioreactor Biofilm contamination and take necessary regulation measure, for prolonging
Slow fouling membrane has important practical significance.
Biomembrane is widely present in nature, and forming process is spontaneous, self-assembling formation process, thus with biomembrane shape
Membrane bioreactor Biofilm contamination as main feature is inevitable.Biofilm contamination includes: (1) microorganism in film surface
Adherency and growth；(2) microorganism and film surface form pollution of the metabolite generated by biomembrane to film in bulk solution.Born of the same parents
Outer polymer (EPS) and biofilm formation are in close relations, and as " biological glue ", one side EPS facilitates microorganism in film table
The attachment in face creates condition for microorganism in the primary growth of film surface；The formation of another aspect film surface biomembrane is strengthened
The ability of film retention larger molecular organics, so that most of dissolubility microbial metabolic products (SMP) are accumulated in reactor,
To further enhanced biological film formation and aggravate membrane biological pollution.Membrane bioreactor Biofilm contamination is a dynamic
, complicated and relatively slow process, be related to quorum sensing mechanism (Bioresource Technology, 2014,171:
472-476), microbial metabolic products (Water Research, 2009,43:1489-512) and it can extract extracellular deoxyribose
Nucleic acid (eDNA) controls mechanism of biofilm formation (Science, 2002,295:1487-1487) etc..Although in membrane bioreactor
Middle Biofilm contamination mechanism is intricate, however a large number of studies show that, eDNA plays very heavy during biofilm formation
The role wanted, it is most important for the formation of biomembrane.EDNA is the non-polymeric metabolite of bacterium, crucial group as EPS
Point, the space structure for maintaining EPS plays key player, plays the function of " scaffold " in biofilm formation process.
Using inhibiting biofilm formation to be concerned to slow down the research of membrane pollution of membrane bioreactor, such as United States Patent (USP)
(No.6,777,223) is disclosed using decomposing enzyme is quenched inhibiting the formation of biomembrane for acyl homoserine lactones (AHL),
However it is larger by environmental factor influence of fluctuations to be quenched enzyme, enzyme easy in inactivation, thus inhibits biomembrane using Quorum quenching enzymes
Application prospect is dull in practical projects for the method for pollution.Korean Patent (No.981519), which is disclosed, is quenched enzyme packet using group
It overlays on magnetic carrier, solves the problems, such as that quencher is lost and recycles, however fundamentally solve quencher easy in inactivation, use
Measure the bottleneck problems such as big and at high cost.The invention proposes membrane bioreactor Biofilm contamination disintegration agent preparation and application sides
Method is proposed and is added into membrane bioreactor backwashing water to decompose in conjunction with membrane bioreactor Biofilm contamination feature
EDNA is main syllabus target Biofilm contamination disintegration agent, to realize that membrane pollution of membrane bioreactor slows down with lower cost.
Summary of the invention
The object of the present invention is to propose it is a kind of alleviation membrane bioreactor in Biofilm contamination disintegration agent preparation and its answer
With method, in conjunction with membrane bioreactor operating condition, to improve the deficiencies in the prior art.
The preparation of Biofilm contamination disintegration agent is completed by following steps:
(1) deoxyribonuclease I is dissolved in ultrapure water, is configured to the solution that mass concentration is 10-30mg/L, stirring
It is uniformly stand-by；
(2) D- tyrosine is dissolved in formic acid solution, is configured to the solution that mass concentration is 5-8mg/L, is placed in brown
It is uniformly mixed in vial, stands 1~2h；
(3) step (1) is uniformly mixed with 2 kinds of solution that step (2) is prepared, adjusts pH value between 5-6, activation is added
Agent Mn2+, Mn2+Mass concentration be 10-30mg/L, become Biofilm contamination disintegration agent.
Membrane bioreactor Biofilm contamination disintegration agent application method:
Biofilm contamination disintegration agent is added into membrane bioreactor backwashing water, dosage and backwashing water volume ratio
For 1:100~150.
The principle of the present invention is that the formation of biomembrane depends on the concentration of eDNA, is contained in Biofilm contamination disintegration agent
Deoxyribonuclease I, in Mn2+In the presence of can efficiently cut off double-stranded DNA, to make eDNA fragmentation, lose building biomembrane
Ability；D- tyrosine can be by the remaining biofilm breakup of film surface simultaneously, and reduces the secretion of EPS, to slow down film biology
Reactor organisms fouling membrane.
Advantages of the present invention and the beneficial effect of generation are:
(1) after adding Biofilm contamination disintegration agent into backwashing water, the membrane bioreactor slow stage can significantly be extended
The fouling membrane time, and membrane bioreactor pollutant removal is not had an impact.
(2) deoxyribonuclease I and D- tyrosine, to the key component of membrane bioreactor film surface biofilm formation
Decomposition is played, is used in combination with synergistic effect；Activator Mn simultaneously2+It is equal to deoxyribonuclease I and D- tyrosine
There is activation.
(3) the Biofilm contamination disintegration agent in bulk solution is diffused to, activated sludge multiplication rate can be reduced, is reduced remaining
Sludge discharge.
Detailed description of the invention
Shown attached drawing 1 is implementation result explanatory diagram.It indicates under different operating conditions, when membrane bioreactor transmembrane pressure is with operation
Between variation.Operating condition 1 indicates that Biofilm contamination disintegration agent is not added in backwashing water in Fig. 1, and transmembrane pressure increases with runing time
Long situation；Operating condition 2,3,4 indicates the Biofilm contamination disintegration agent that different proportion is added into backwashing water, and transmembrane pressure is with operation
Time growth pattern.
Implement concrete mode
Below by specific embodiment, preparation proposed by the present invention and application method are further described, implemented
Effect is referring to attached drawing 1.It should be noted that following embodiments be it is narrative, without being restrictive, not with this embodiment limit
Fixed protection scope of the presently claimed invention.Raw material used in embodiment is commercial products.
The preparation of fouling membrane disintegration agent is completed by following steps:
(1) deoxyribonuclease I is dissolved in ultrapure water, is configured to the solution that mass concentration is 10-30mg/L, stirring
It is uniformly stand-by；
(2) D- tyrosine is dissolved in formic acid solution, is configured to the solution that mass concentration is 5-8mg/L, is placed in brown
It is uniformly mixed in container, stands 1~2h；
(3) step (1) is uniformly mixed with 2 kinds of solution that step (2) is prepared, adjusts pH value between 5-6, activation is added
Agent Mn2+, Mn2+Mass concentration is 10-30mg/L, becomes Biofilm contamination disintegration agent.
Membrane bioreactor Biofilm contamination disintegration agent application method:
Prepared Biofilm contamination disintegration agent is added into membrane bioreactor backwashing water, dosage and backwash
Water volume ratio is 1:100~150.
Embodiment 1:
Membrane bioreactor effective volume 5L, sludge concentration fluctuation range be 9~13g/L, hydraulic detention time (HRT) and
Sludge retention time (SRT) is respectively 6h and 30d, sanitary sewage water inlet COD (CODcr) it is 240~460mg/L；Film
Effluent flux is 10L/m2H, in per duty cycle, film suction time is 13min, idle hours 2min, and the stage of resting carries out
Backwash, backwash flow are 5L/m2h。
(1) the deoxyribonuclease I solution that mass concentration is 10mg/L is prepared first, is stirred evenly stand-by；
(2) D- tyrosine is dissolved in formic acid solution, being configured to mass concentration is 5mg/L solution, is placed in brown glass
It is uniformly mixed in bottle, stands 1h；
(3) above-mentioned 2 kinds of solution is uniformly mixed, adjusting pH value is 5.4, and Mn is added2+Mass concentration is 10mg/L, and mixing is equal
It is even stand-by.
Prepared Biofilm contamination disintegration agent is added into membrane bioreactor backwashing water, dosage and backwash
Water volume ratio is 1:100.
Implementation result is shown in 1 operating condition 2 of attached drawing, and compared with not adding Biofilm contamination disintegration agent (operating condition 1), the present embodiment is significant
Slow fouling membrane phases-time is extended, membrane fouling rate is effectively reduced.
Embodiment 2:
In the present embodiment, reactor used structure is same as Example 1.Sludge concentration fluctuation range is 8~11g/L, surely
Determine stage running parameter: HRT 6h, SRT 25d, sanitary sewage influent CODcrFor 390mg/L~560mg/L；Film service condition
It is same as Example 1.
(1) the deoxyribonuclease I solution that mass concentration is 20mg/L is prepared first, is stirred evenly stand-by；
(2) D- tyrosine is dissolved in formic acid solution, being configured to mass concentration is 7mg/L solution, is placed in brown glass
It is uniformly mixed in bottle, stands 1.5h；
(3) step (1) is uniformly mixed with 2 kinds of solution that step (2) is prepared, adjusting pH value is 5.2, and Mn is added2+Quality
Concentration is 15mg/L.
Prepared Biofilm contamination disintegration agent is added into membrane bioreactor backwashing water, dosage and backwash
Water volume ratio is 1:120.
Implementation result is shown in 1 operating condition 3 of attached drawing, and compared with not adding Biofilm contamination disintegration agent (operating condition 1), the present embodiment is obvious
Slow fouling membrane phases-time is extended, membrane fouling rate is effectively reduced.
Embodiment 3:
In the present embodiment, reactor used structure and sanitary sewage water quality are same as Example 2.Sludge concentration fluctuation range
For 8~11g/L, operating parameter: HRT 8h, SRT 20d；Film service condition is same as Example 1.
(1) the deoxyribonuclease I solution that mass concentration is 30mg/L is prepared first, is stirred evenly stand-by；
(2) D- tyrosine is dissolved in formic acid solution, being configured to mass concentration is 8mg/L solution, is placed in brown glass
It is uniformly mixed in bottle, stands 2h；
(3) step (1) is uniformly mixed with 2 kinds of solution that step (2) is prepared, adjusting pH value is 5.7, and Mn is added2+Quality
Concentration is 30mg/L.
Prepared Biofilm contamination disintegration agent is added into membrane bioreactor backwashing water, dosage and backwash
Water volume ratio is 1:150.
Implementation result is shown in 1 operating condition 4 of attached drawing, and compared with not adding Biofilm contamination disintegration agent (operating condition 1), the present embodiment is significant
Slow fouling membrane phases-time is extended, membrane fouling rate is effectively reduced.
After disintegration agent is added as seen from Figure 1, the transmembrane pressure rate of changing with time is obviously reduced, and transmembrane pressure is at any time
It is better to change the smaller effect for illustrating decelerating membrane pollution, especially operating condition 4 is compared with operating condition 1, effect highly significant.
Claims (2)
1. a kind of disintegration agent for alleviating Biofilm contamination in membrane bioreactor, it is characterised in that: Biofilm contamination disintegration agent
Preparation is completed by following steps:
(1) deoxyribonuclease I is dissolved in ultrapure water, is configured to the solution that mass concentration is 10-30mg/L, stirs evenly
For use；
(2) D- tyrosine is dissolved in formic acid solution, is configured to the solution that mass concentration is 5-8mg/L, is placed in brown glass
It is uniformly mixed in bottle, stands 1~2h；
(3) step (1) is uniformly mixed with 2 kinds of solution that step (2) is prepared, adjusts pH value between 5-6, activator Mn is added2 +, Mn2+Mass concentration be 10-30mg/L, become Biofilm contamination disintegration agent.
2. a kind of disintegration agent application method according to Biofilm contamination in a kind of alleviation membrane bioreactor described in claim 1,
It is characterized in that: Biofilm contamination disintegration agent is added into membrane bioreactor backwashing water, dosage and backwashing water volume
Than for 1:100~150.
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| CN108753632A (en) * | 2018-05-25 | 2018-11-06 | 安徽中医药大学 | A method of dissolving candida albicans biofilm extracellular matrix |
| CN117886438A (en) * | 2024-01-29 | 2024-04-16 | 上海海洋大学 | Lanthanide compound-based MBR membrane pollution inhibition system and method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101040052A (en) * | 2004-09-10 | 2007-09-19 | 诺维信北美公司 | Methods for preventing, removing, reducing, or disrupting biofilm |
| CN103734133A (en) * | 2013-09-13 | 2014-04-23 | 华中科技大学 | Amino acid composite biomembrane remover |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101040052A (en) * | 2004-09-10 | 2007-09-19 | 诺维信北美公司 | Methods for preventing, removing, reducing, or disrupting biofilm |
| CN103734133A (en) * | 2013-09-13 | 2014-04-23 | 华中科技大学 | Amino acid composite biomembrane remover |
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