CN106568711A - Biochemical test piece - Google Patents
Biochemical test piece Download PDFInfo
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- CN106568711A CN106568711A CN201510644752.6A CN201510644752A CN106568711A CN 106568711 A CN106568711 A CN 106568711A CN 201510644752 A CN201510644752 A CN 201510644752A CN 106568711 A CN106568711 A CN 106568711A
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- peroxidating
- tributyl
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Abstract
The invention provides a biochemical test piece, which comprises an insulation substrate, an electrode unit, a first insulation partition, a reaction layer, a second insulation partition and an electrochromic layer. The electrode unit is positioned on the insulation substrate. The first insulation partition is positioned on the electrode unit and has an opening. The opening exposes part of the electrode unit. The reaction layer is positioned in the opening. The second insulation partition is positioned on the first insulation partition. The electrochromic layer is at least arranged on the area outside the reaction layer. The color of the electrochromic layer changes with the change of the environmental factor. The biochemical test piece provided by the invention can sense changes of the environmental factor. Without placing a sample into the reaction zone, whether the biochemical test piece is damaged can be prompted for a user.
Description
Technical field
The invention relates to a kind of biochemical test piece, and in particular to a kind of with conjugated polymer
Biochemical test piece.
Background technology
Be in conventional biochemical sensor using comprising the conversion zone of composition such as enzyme, conducting medium, buffer with
Sample is reacted, and the biochemical concentration produced by the reaction is analyzed by optics or electrochemical means.So
And, the composition of above-mentioned conversion zone is extremely sensitive for environmental factorss such as light, temperature, humidity, oxygen contents.
For example, if enzyme is in non-optimum temperature environment, it will be lost should be active.In addition, conductive be situated between
Matter as illumination and produce structure change, such as ferrous compound can be become three after ultraviolet light
Valency iron compound.And the change that above-mentioned conversion zone is produced because of environmental factorss, belonging to mostly can not be converse
Should, it will cause biochemical sensor to lose original measurement function.
In order to solve impact of the environmental factorss to biochemical sensor, often using such as aluminium film or containing desiccant not
Transparent vessel deposits biochemical sensor to wrap.However, the packing method is only capable of carrying out limited isolation (for example
Light or humidity etc.), it fails impact of the effectively solving temperature to biochemical sensor, and does not make with prompting
The function that the user biochemical sensor has been damaged because of temperature.
Although separately there is dealer to design multiple reaction zone biochemical sensors, it can be by the concentration of two reaction zones
Whether measurement is impaired or chemical reaction medicament is mixed in conversion zone to analyze the biochemical sensor, to measure
Whether conversion zone medicament is damaged.But above two method is both needed to after user places a sample into reaction zone
Can learn whether the sensor is in damaged condition, and then cause the unnecessary puzzlement of user.Therefore, how to send out
A kind of biochemical test piece is opened up, it can sense the change of environmental factorss, and need not place a sample into reaction zone just
Can point out whether the user biochemical test piece is impaired will to become following important subject topic.
The content of the invention
The present invention provides a kind of biochemical test piece, and it can sense the change of environmental factorss and need not put in sample
Entering reaction zone just can point out whether biochemical test piece described in user is damaged.
The present invention provides a kind of biochemical test piece, including insulated substrate, electrode unit, the first insulating spacer,
Conversion zone, the second insulating spacer and photochromic layer.Electrode unit is located on insulated substrate.First insulation every
Piece is located on electrode unit and with opening.Opening expose portion electrode unit.Conversion zone is located in opening.
Second insulating spacer is located on the first insulating spacer.Photochromic layer is at least configured on the region outside conversion zone.
The color of photochromic layer changes with the change of environmental factorss.
In one embodiment of this invention, the photochromic layer be configured in the insulated substrate upside or downside,
The upside or downside of first insulating spacer, or the upside or downside of second insulating spacer.
In one embodiment of this invention, the material of the photochromic layer includes conjugated polymer.
In one embodiment of this invention, the conjugated polymer includes arene compound, non-aromatic hydrocarbon
Compound, anti-arene compound or its combination.
In one embodiment of this invention, the arene compound includes polycyclic aromatic compound, benzene
Based compound, crystal violet derivant or its combination.
In one embodiment of this invention, the polycyclic aromatic compound include arylmethane compounds,
Fluoran compound, phenothiazine compounds, thio fluoran compound, cluck ton compound, spiropyran compounds,
.alpha.-5:6-benzopyran pyrazole compound, methylidyne compound, rhodamine lactam compound, quinazoline compound,
Diaza cluck ton compound, dilactone compound or its combination.
In one embodiment of this invention, the phenyl compound includes cresol red derivant, thymol blue
Derivant, aniline yellow derivant, 2,4- dinitro benzene amphyls, bromophenol blue derivant, methyl yellow derive
Thing, bromocresol green derivant, C.I. 13020. derivant, C.I. 14645. derivant, bromocresol purple derivant, madder
Red derivant, a nitre phenol derivatives, o- cresol phthalide derivatives, phenolphthalein derivant, winter daphne phenolphthalein derive
Thing, alizarin yellow derivant R, indigo carminum derivant, malachite green oxalate derivant, trisbromomethyl benzene sulfone derive
Thing, victoria blue B derivant, Victoria green G derivative, phthalocyanine blue derivant, phthalocyanine green are spread out
Biological or its combination.
In one embodiment of this invention, the photochromic layer also includes monomer.The monomer includes noncrystalline
Polymer, chlorostyrene, ethylene, propylene, butylene, stretch isopentyl, vinyl acetate, vinyl propionate
Ester, vinyl benzoate, vinyl butyrate, acrylic acid methyl ester., ethyl acrylate, alkene butyl acrylate,
2-EHA, phenyl acrylate, methyl methacrylate, ethyl methacrylate, methyl
Butyl acrylate, vinyl methylether, ethoxy ethylene, butyl vinyl ether, methyl vinyl ketone, levulinic
Ketone, polyethylene and polypropylene, polyester resin, polyurethane resins, epoxy resin, silica tree
Fat, improved resin, paraffin or its combination.
In one embodiment of this invention, the photochromic layer also includes sensitizer.The sensitizer is included not
Saturated ketone, 1,2- derovatives, benzo radical derivative, fluorene derivative, naphthoquinone derivatives, anthraquinone spread out
Spend cyanines derivant, many in mutter derivant, coumarin derivative, flower cyanines derivant, portion of biological, dibenzo piperazine
Methine derivative, acridine derivatives, azepine benzene derivative, oxazines derivative, indoline derivative thing,
Azulene derivatives, derivatives of porphyrin, four benzene derivatives of porphyrin, triaryl methane derivant, phthalocyanine derivates,
Wheel ene derivative, spiropyran derivatives, spirooxazine derivant, organic ruthenium misfit thing or its combination.
In one embodiment of this invention, the unsaturated ketone includes chromocor derivative, Er Ya Benzyl acetone
Or its combination, 1, the 2- derovatives Bao Kuo Benzyl bases, camphorquinone or its combination.
In one embodiment of this invention, the forming method of the photochromic layer includes cladding process, ink jet printing
Method, twine print process or distributing print process.
In one embodiment of this invention, the environmental factorss include light, temperature, humidity, oxygen content or
Its combination.
In one embodiment of this invention, the photochromic layer color change show word, numeral, figure,
Pattern, symbol or its combination.
In one embodiment of this invention, the photochromic layer also includes initiator, and the initiator is included
Machine peroxide.
In one embodiment of this invention, the organic peroxide include isobutyl peroxide, α, α '-
Double (new capryl peroxidating) diisopropyl benzenes, peroxidating neodecanoic acid cumyl ester, peroxy dicarbonate be double-
N-propyl, peroxy dicarbonate double-the second butyl ester, neodecanoic acid 1,1,3,3- tetramethyl butyl esters, the carbon of peroxidating two
Sour double (4- tributyl cyclohexyl) ester, peroxidating neodecanoic acid 1- cyclohexyl -1- Methylethyls, peroxidating
Double -2- the ethoxy ethyl esters of two carbonic acid, double (ethylhexyl peroxidating) two carbonic esters, the own ester of neodecanoic acid the 3rd,
Peroxy dicarbonate bi-methoxy butyl ester, double (3- methyl -3- methoxybutyl peroxidating) two carbonic esters,
Peroxidating neodecanoic acid tributyl, the own ester of peroxidating pivalic acid the 3rd, 3,5,5- trimethyl acetyl base peroxidating
Thing, sim peroxides, lauroyl peroxide, stearyl peroxide, peroxidating -2- second
Base caproic acid 1,1,3,3- tetramethyl butyl esters, succinyl peroxide, 2,5- dimethyl -2,5- are double (2- ethyl hexyl acyl groups)
Hexane, peroxidating -2 ethyl hexanoic acid 1- cyclohexyl -1- Methylethyls, the own ester of peroxidating -2 ethyl hexanoic acid the 3rd,
4- methyl benzoyl peroxide, peroxidating -2 ethyl hexanoic acid tributyl, a toluyl groups benzene first
Double (the 3rd fourths of acyl peroxide, benzoyl peroxide, peroxidating isopropylformic acid. tributyl, 1,1-
Base peroxidating) 2- hexahydrotoluenes, double (the 3rd hexyl the peroxidating) -3,3,5- trimethyl-cyclohexanes of 1,1-,
Double (the 3rd hexyl peroxidating) hexamethylene of 1,1-, double (tributyl the peroxidating) -3,3,5- trimethyls of 1,1-
Double (tributyl peroxidating) Ketohexamethylene of hexamethylene, 1,1-, double (the 4,4- dibutyl peroxidating hexamethylenes of 2,2-
Base) propane, double (tributyl peroxidating) cyclododecanes of 1,1-, isopropyl peroxide single carbon acid the 3rd oneself
Ester, tributyl peroxidating maleic acid, peroxidating -3,5,5 Trimethylhexanoic acid tributyl, peroxidating
Double (a toluyl groups peroxidating) hexanes of lauric acid tributyl, 2,5- dimethyl -2,5-, peroxidating are different
Propyl group single carbon acid tributyl, peroxidating -2- ethylhexyls single carbon acid tributyl, perbenzoic acid the
Double (benzoylperoxy) hexane of three own esters, 2,5- dimethyl -2,5-, peracetic acid tributyl,
Double (tributyl peroxidating) butane of 2,2-, perbenzoic acid tributyl, the double (tributyls of 4,4-
Peroxidating) n-butyl pentanoate, the tributyl of peroxidating M-phthalic acid two-the, α, α '-bis- (tributyl mistakes
Oxidation) diisopropyl benzene, cumyl peroxide, 2,5- dimethyl -2,5- double (tributyl peroxidating)
Hexane, tributyl dicumyl peroxide, double to terpane hydroperoxides, 2,5- dimethyl -2,5- (the
Tributyl peroxidating) hexin, diisopropyl benzene hydroperoxides, tributyl TMS peroxide
Compound, 1,1,3,3- tetramethyl butyl hydroperoxides, cumene hydroperoxide, the 3rd hexyl hydrogen peroxide
Compound, tributyl hydroperoxides, 2,3- dimethyl -2,3- diphenyl butanes or its combination.
In one embodiment of this invention, the activity change speed of the conversion zone is equal to the photochromic layer
Concentration change speed.
In one embodiment of this invention, the concentration change speed of the photochromic layer be by formula (1) with
Formula (2) is defining, and its is as follows：
Wherein
K is the reaction rate constant of the photochromic layer, and it is defined by formula (2)；
A, b are experimental constant, and wherein a is the rate of change constant K and temperature T of the photochromic layer
Intercept, b is the slopes of the rate of change constant K with temperature T of the photochromic layer；
C0For the original concentration of the photochromic layer；
CiFor the photochromic layer varied concentration to be presented, wherein the varied concentration is defined as the reaction
Layer loses varied concentration during original measurement function；
A is the reaction rate of the conversion zone or the photochromic layer at temperature T.
In one embodiment of this invention, the reaction rate of the photochromic layer at a certain temperature is normal
Number is adjusted by the concentration of the compound in the photochromic layer.
In one embodiment of this invention, the compound in the photochromic layer include conjugated polymer,
Monomer, sensitizer, initiator or its combination.
Based on above-mentioned, the present invention is using being at least configured on the region outside conversion zone and with conjugated polymers
The photochromic layer of thing, its color can change with the change of environmental factorss.And the color change of photochromic layer
Various words, numeral, figure (figure), pattern (pattern), symbol etc. can be shown as.Therefore,
User need not place a sample into reaction zone and just can learn whether the biochemical test piece is damaged, and it not only may be used
The consume of sample is reduced, the risk of the detection error of biochemical test piece can be also reduced.
It is that the features described above and advantage of the present invention can be become apparent, special embodiment below, and coordinate
Accompanying drawing is described in detail below.
Description of the drawings
Fig. 1 is the exploded perspective view according to the biochemical test piece of one embodiment of the invention；
Fig. 2 is the loss of activity figure of the conversion zone of experimental example 1-2.
Description of reference numerals：
100：Biochemical test piece；
110：Insulated substrate；
120：Electrode unit；
122：Working electrode；
124：Reference electrode；
126、128：Identification electrode；
130：First insulating spacer；
132：Opening；
140：Conversion zone；
142：Reaction zone；
150：Second insulating spacer；
152：The transparent area of observation coverage；
160：Photochromic layer.
Specific embodiment
Fig. 1 is the exploded perspective view according to the biochemical test piece of one embodiment of the invention.
Fig. 1 is refer to, the present invention provides a kind of biochemical test piece 100, including insulated substrate 110, electrode list
First 120, first insulating spacer 130, conversion zone 140, the second insulating spacer 150 and photochromic layer 160.
In the present embodiment, biochemical test piece 100 is the electrochemistry test piece of the blood sample for receiving user,
To the numerical value for measuring Blood Glucose, cholesterol, uric acid, lactic acid, haemachrome etc..But the present invention is simultaneously
Not limited to this, in other embodiments, biochemical test piece 100 can also be used in any liquid sample, as long as
Electrochemical reaction can be produced with conversion zone 140 or distinguish the ability of biomaterial or signal with specificity
.
Insulated substrate 110 is with even curface, with electrical insulating property and is resistant to 40 DEG C～120 DEG C
The base material of temperature capacity.In one embodiment, the material of insulated substrate 110 can comprising polrvinyl chloride (PVC),
It is glass fibre (FR-4), polyester (polyester suphone), bakelite plate (bakelite), poly- to benzene two
It is formic acid diethylester (PET), Merlon (PC), polypropylene (PP), polyethylene (PE), poly-
The combination in any of styrene (PS), glass plate, ceramics or above-mentioned material.Certainly, insulated substrate 110
Material be not limited to that.
As shown in figure 1, electrode unit 120 is located on insulated substrate 110.Electrode unit 120 is comprising mutual
Working electrode 122, reference electrode 124 and the identification electrode 126,128 for mutually insulating.In the present embodiment
In, identification electrode 126,128 is configured at working electrode 122, the outside of reference electrode 124.But electrode
The configuration of unit 120 can in response to various demands different, the arrangement being not intended to limit between these electrodes,
The quantity of electrode is not limited, designer can increase and decrease electrode according to needed for practical application, and the present invention is not yet
It is limited to this.
In the present embodiment, recognizing electrode 126,128 can be by (can example from opening 132 in successive process
Thief hatch in this way) liquid sample that enters and turn on, thereby start measuring process.And working electrode 122
With reference electrode 124 be then judge the liquid sample that enters in successive process whether with conversion zone 140
It is electrochemically reacted or produces specificity and distinguishes bio signal.But the present invention is not limited to this, another
In one embodiment, being possible with electrode 126,128 carries out the measurement of chaff interference.For example, electrode is worked as
122nd, 124 when blood glucose measurement is carried out, and it can correct blood glucose value by the measured value of chaff interference.It is another
Aspect, in other embodiments, also can carry out the first sample concentration detection by electrode 126,128, and
Electrode 122,124 carries out the second sample concentration detection.The material of electrode unit 120 can be any conducting objects
Matter, such as palladium glue, platinum glue, gold size, titanium glue, carbon paste, elargol, copper glue, gold silver epoxy glue, carbon silver
The combination in any of epoxy glue or above-mentioned conductive material.In one embodiment, electrode unit 120 is by one
Conductive carbon bisque is constituted.In another embodiment, electrode unit 120 is made up of a metal level.
In another embodiment, electrode unit 120 is by a conductive silver glue-line and conductive carbon bisque institute disposed thereon
Composition, the impedance of above-mentioned conductive carbon bisque is typically much deeper than conductive silver glue-line or other metal glue-lines.
First insulating spacer 130 is located on electrode unit 120.First insulating spacer 130 has opening 132,
At least expose portion working electrode 122 of opening 132 and reference electrode 124.In the present embodiment, it is open
As long as 132 can expose the part working electrode 122 and reference electrode 124 needed for measurement, the present invention
It is not intended to limit the area and shape of opening 132.In one embodiment, the material of the first insulating spacer 130
Can be including but not limited to polyvinyl chloride insulation adhesive tape, ethylene terephthalate insulating tape, heated drying type
Insullac or ultraviolet curing type insullac.
Conversion zone 140 is located in opening 132.Conversion zone 140 at least covers the work corresponding to opening 132
Make electrode 122 and reference electrode 124, to be electrochemically reacted or produce specificity biological letter is distinguished
Number.In detail, conversion zone 140 is including at least oxidoreductase and electron transfer instrumentality.Oxidation
Reductase is to target analytes (can be for example glucose, cholesterol, uric acid, lactic acid, haemachrome etc.)
Specificity with height, is only catalyzed the reaction configuration of target analytes and with catalysis target analytes
Macromolecule composite spherical protein.
In another embodiment, conversion zone 140 may also comprise the coenzyme (coenzyme) for participating in reaction,
It is a special subset prothetic group, and its cofactor (or can be described as cofactor) can be sticked tightly in oxidoreduction
On enzyme, and do not consume because of above-mentioned reaction, and because whole reaction belongs to coupling reaction (coupling
Reaction), thus the consumption of coenzyme or growing amount can also bring the catalysis activity for judging oxidoreductase.
In one embodiment, coenzyme can be for example the core Enzyme of flavin gland alkali two acid (Flavin-adenine dinucleotide)
Or nicotinamide adenine dinucleotide (Nicotinamide adenine dinucleotide), but the present invention is not
As limit.
When the target analytes and oxidoreductase metabolite react so that the cofactor reduction of coenzyme,
And electron transfer instrumentality is then coenzyme is returned back to into its dehydrogenated state.In other words, the coenzyme and
Electron transfer instrumentality is reduced, and the electrons being reduced diffuse to biochemical sensor electrode surface and shape
Into a potential difference.By measure the potential difference and quantitative analyses target analytes content.In one embodiment,
Electron transfer instrumentality can be for example Ferrocene and its derivative, quinine and its derivant, organic conductive
Salt or viologen (viologen), chlorination pregnancy urotropine ruthenium (III), the potassium ferricyanide, potassium ferrocyanide
Or it is combined, but the present invention is not limited.
Second insulating spacer 150 is located in the first insulating spacer 130 and conversion zone 140.Due to second
Insulating spacer 150 is completely covered in conversion zone 140, and it causes conversion zone 140 upper and lower and three
Side wall (in addition to thief hatch) is insulated by the second insulating spacer 150, insulated substrate 110 and first
The tubular space (not shown) that partition 130 is surrounded and formed.When liquid sample enters above described tubular space
When, adhesive force of the liquid sample in tubular space can be more than the cohesiveness of liquid sample, and cause liquid
Sample is continuously advanced.Now, liquid sample can be contacted with the conversion zone 140 in tubular space so that liquid
Aspect product mix with the oxidoreductase in conversion zone 140, coenzyme and electron transfer instrumentality, with
Reaction zone 142 is formed in tubular space.
Additionally, in order to allow user to see that liquid sample injects the situation of reaction zone 142, in this reality
In applying example, the second insulating spacer 150 has the transparent area of observation coverage 152.The transparent area of observation coverage 152 exposes at least
A part reaction zone 142, in order to observe reaction zone 142 in liquid sample injection situation.One
In embodiment, the second insulating spacer 150 also can be for example transparent upper cover, and it may be used to observe photochromic layer 160
Color change.
It should be noted that in the present embodiment, photochromic layer 160 is at least configured in outside conversion zone 140
Region on.The color of photochromic layer 160 can change with the change of environmental factorss.For example, such as
Shown in Fig. 1, photochromic layer 160 is configured on the upside of the first insulating spacer 130.Due to photochromic layer 160
Material include conjugated polymer, the conjugated polymer and conversion zone 140 have identical change speed,
And photochromic layer 160 and conversion zone 140 under same environmental condition (namely light, temperature, humidity,
Oxygen content is identical).Therefore, change when the conjugated polymer produces color with the change of environmental factorss
During change, also illustrate that oxidoreductase in conversion zone 140, coenzyme or electron transfer instrumentality also with when
Between environmental factorss and loss of activity or produce structure change.Consequently, it is possible to user is only needed using print in advance
It is formed on test piece tank, description or learns the present embodiment by the ratio that shown by measuring instrument screen is checked colors
Whether biochemical test piece 100 is damaged.In one embodiment, the conjugated polymer can be for example fragrant hydrocarbonylation
Compound, non-aromatic compounds, anti-arene compound or its combination.The arene compound can example
Polycyclic aromatic compound (Polycyclic aromatic hydrocarbon compound), phenyl in this way
Compound, crystal violet derivant (Crystal Violet derivative) or its combination.The multi-ring fragrance
Hydrocarbon compound can be for example arylmethane compounds (triarylmethane compound), fluoran compound
It is (fluorine compound), phenothiazine compounds (phenothiazine compound), thio glimmering
Hydride compounds (thiofluoran compound), cluck ton compound (xanthene compound), spiral shell
Pyrylium compound (spiropyran compound) .alpha.-5:6-benzopyran pyrazole compound (chromenopyrazole
Compound), methylidyne compound (methine compound), rhodamine lactam compound
(rhodaminelactam compound), quinazoline compound (quinazoline compound), two
Azepine cluck ton compound (diazaxanthene compound), dilactone compound (bislactone
Compound) or its combination.The phenyl compound can be for example cresol red derivant (cresol red
Derivative), thymol blue derivant (Thymol Blue derivative), aniline yellow derivant (aniline
Yellow derivative), 2,4- dinitro benzene amphyls (2,4-Dinitrophenol derivative),
Bromophenol blue derivant (Bromophenol blue derivative), methyl yellow derivant (Methyl Orange
Derivative), bromocresol green derivant (bromcresol green derivative), C.I. 13020. derivant
(Methyl Red derivative), C.I. 14645. derivant (Eriochrome Black T derivative),
Bromocresol purple derivant (bromcresol purple derivative), alizarin red derivant (Alizarin
Derivative), a nitre phenol derivatives (m-nitrophenol derivative), o- cresol phthalide derivatives
(o-Cresolphthalein derivative), phenolphthalein derivant (Phenolphthalein derivative),
Daphne phenol phthalide derivative (Thymolphthalein derivative), alizarin yellow derivant R (Alizarin
Yellow R derivative), indigo carminum derivant (Indigo Carmine derivative), malachite green oxalate
Derivant (Malachite green derivative), trisbromomethyl Benzosulfone derivatives (Phenyl
Tribromomethyl sulfone derivative), victoria blue B derivant (Victoria blue B
Derivative), Victoria green G derivative (Victoria green G derivative), phthalocyanine blue are spread out
Biological (Phthalocyanine derivative), phthalocyanine green derivant (Phthaloyanine Green G
Derivative) or its combination.
In one embodiment, the forming method of photochromic layer 160 may include cladding process, ink jet printing method, net
Silk print process or distributing print process.For example, can be by the conjugated polymer of liquid form with nozzle painting
Overlay on upside or downside, the upside of the first insulating spacer 130 or the downside of insulated substrate 110, or second
The upside or downside of insulating spacer 150.But the present invention is not limited, as long as by the conjugated polymer
It is coated on the region beyond conversion zone 140.Then, then by the conjugated polymers of the liquid form
Thing is transformed into the conjugated polymer of solid-state form.In one embodiment, the mode of the transformation can be for example
Spontaneously dry or dry etc., the present invention is not limited.
Further, since photochromic layer 160 can be formed using above-mentioned various methods, therefore, when photochromic layer 160
Color when changing with the change of environmental factorss, the color change of photochromic layer 160 can be shown that word,
Numeral, figure, pattern, symbol or its combination.Consequently, it is possible to user need not be placed a sample into instead
Answer area just can learn whether the biochemical test piece is damaged, it can not only reduce the consume of sample, can also increase
The detection efficiency of biochemical test piece.In one embodiment, the environmental factorss can be for example light, temperature, wet
Degree, oxygen content or its combination.But the present invention is not limited, as long as can cause in conversion zone 140
The environmental factorss of oxidoreductase, coenzyme or electron transfer instrumentality loss of activity or generation structure change are equal
In scope of the invention.
Additionally, the present invention is not intended to limit the discrimination method of the discoloration of the conjugated polymer in photochromic layer 160.
In one embodiment, (one is intact for biochemical test piece than checking colors biochemistry can be tried into printed on chip at least two
Color, another color in damaged condition for biochemical test piece), and during photochromic layer is then checked colors positioned at two ratios
Between (not shown), user can recognize whether this biochemical test piece is damaged than checking colors by described.Another
One embodiment, then be the bonding pad (not shown) that photochromic layer can be arranged at biochemical test piece, when biochemical examination
After piece insertion measuring instrument, measuring instrument can read the conjugated polymer in photochromic layer and be presented by optical system
Color, and by show or voice mode to alert the user biochemical test piece in damaged condition.In an embodiment
In, the biochemical sensor is not limited to biochemical test piece, and it also can be for example biochemical cassette.
In the present embodiment, photochromic layer 160 can also include monomer or sensitizer.The monomer may be used to increase
Plus the stability of the conjugated polymer in the color-identifying degree and increase photochromic layer 160 of photochromic layer 160.
In other words, the present embodiment can be by addition different content or different types of monomer, to form different colours
Photochromic layer 160, its cause it is altered in photochromic layer 160 after color identification it is also different therewith.
In one embodiment, the monomer can be for example noncrystalline polymer (amorphous polymer), chlorine
Styrene (chlorostyrene), ethylene (ethylene), propylene (propene), butylene (butene),
Stretch isopentyl (isoamylene), vinyl acetate (vinyl acetate), propionate (vinyl
Propionate), vinyl benzoate (vinyl benzoate), vinyl butyrate (vinyl butyrate),
Acrylic acid methyl ester. (methyl acrylate), ethyl acrylate (ethyl acrylate), alkene butyl acrylate
(butyl acrylate), 2-EHA (2-ethylhexyl acrylate), phenyl acrylate
(phenyl acrylate), methyl methacrylate (methyl methacrylate), methacrylic acid second
Ester (ethyl methacrylate), butyl methacrylate (butyl methacrylate), vinyl methylether
(vinyl methyl ether), ethoxy ethylene (ethoxyethene), butyl vinyl ether (butyl vinyl
Ether), methyl vinyl ketone (methyl vinyl ketone), acetylacetone,2,4-pentanedione (acetylacetone),
Polyethylene (polyvinyl) and polypropylene (polypropylene), polyester resin (polyester resin),
Polyurethane resins (polyurethane resin), epoxy resin (epoxy resin), silica
Resin (silicone resin), improved resin (modified resin), paraffin (paraffins) or its group
Close.
In the present embodiment, in order in response to the reaction of 140 pairs of environmental condition of differential responses layer, can be in discoloration
Layer 160 in add the sensitizer, with adjust conjugated polymer active-energy cationic polymerization and/or
The reactivity of radical polymerization.The sensitizer can be for example unsaturated ketone, 1,2- derovatives
(1,2-diketone derivative), benzo radical derivative (benzo derivative), fluorene derivative (fluorine
Derivative), naphthoquinone derivatives (naphthoquinone derivative), anthraquinone derivative
(anthraquinone derivative), dibenzo croak mutter derivant (xanthenes derivative), fragrant
Legumin derivant (coumarin derivative), flower cyanines derivant (gallocyanine derivative),
Spend cyanines derivant (merocyanine-based derivative), polymethine derivant (polymethine in portion
Derivative), acridine derivatives (acridine derivative), azepine benzene derivative (pyridazine
Derivative), oxazines derivative (oxazine derivative), indoline derivative thing (indoline
Derivative), azulene derivatives (azulene derivative), derivatives of porphyrin (porphyrins derivative),
Four benzene derivatives of porphyrin (tetraphenyl porphyrin derivative), triaryl methane derivant
(triarylmethane derivative), phthalocyanine derivates (phthalocyanin derivative), annulene
Derivant (annulene derivative), spiropyran derivatives (spiropyrans derivative), spiral shell
Oxazines derivative (spirooxazine derivative), organic ruthenium misfit thing (ruthenium complexes)
Or its combination.The unsaturated ketone can be for example chromocor derivative (flavones derivative), two sub-
Benzyl acetone (dibenzalacetone) or its combination, 1, the 2- derovatives (1,2-diketone
Derivative) can Li such as Shi Benzyl bases (benzyl derivative), camphorquinone (camphorquinone
Derivative) or its combination.
In addition, in the present embodiment, initiator can be added in photochromic layer 160.The initiator can be by
The conjugated polymer radical polymerization, to adjust the reaction rate constant of the photochromic layer 160.It is described
Initiator can be for example the organic peroxide that free free radical is produced by specific temperature conditions, but this
Invention not limited to this.The initiator can be for example isobutyl peroxide (isobutyl peroxide), α, and α '-
Double (new capryl peroxidating) diisopropyl benzene (α, α '-bis (neodecanoyl peroxy)
Diisopropylbenzene), peroxidating neodecanoic acid cumyl ester (cumyl peroxyneodecanoate),
Peroxy dicarbonate is double-and n-propyl (bis-n-propyl peroxydicarbonate), peroxy dicarbonate be double-
Second butyl ester (bis-s-butyl peroxydicarbonate), neodecanoic acid 1,1,3,3- tetramethyls butyl ester (1,1,3,3
- tetramethylbutyl neodecanoate), double (the 4- tributyl cyclohexyl) ester (bis of peroxy dicarbonate
(4-t-butyl cyclohexyl) peroxydicarbonate), peroxidating neodecanoic acid 1- cyclohexyl -1- methyl
Double-the 2- of ethyl ester (1-cyclohexyl-1-methyl ethyl peroxyneodecanoate), peroxy dicarbonate
Ethoxy ethyl ester (bis-2-ethoxy ethyl peroxydicarbonate), double (ethylhexyl peroxidating)
Two carbonic esters (bis (ethyl hexyl peroxy) dicarbonate), the own ester (t-hexyl of neodecanoic acid the 3rd
Neodecanoate), peroxy dicarbonate bi-methoxy butyl ester (bismethoxy butyl
Peroxydicarbonate), double (3- methyl -3- methoxybutyl peroxidating) two carbonic ester (bis
(3-methyl-3-methoxy butyl peroxy) dicarbonate), peroxidating neodecanoic acid tributyl,
The own ester (t-hexyl peroxypivalate) of peroxidating pivalic acid the 3rd, 3,5,5- trimethyl acetyl base peroxidating
Thing (3,5,5-trimethyl hexanoyl peroxide), sim peroxides (octanoyl peroxide),
Lauroyl peroxide (lauroyl peroxide), stearyl peroxide (stearoyl peroxide),
Peroxidating -2 ethyl hexanoic acid 1,1,3,3- tetramethyl butyl esters (1,1,3,3-tetramethyl butyl peroxy-2-ethyl
Hexanoate), double (the 2- ethyls of succinyl peroxide (succinic peroxide), 2,5- dimethyl -2,5-
Caproyl) hexane (2,5-dimethyl-2,5-bis (2-ethylhexanoyl) hexane), peroxidating -2-
Thylhexoic acid 1- cyclohexyl -1- Methylethyl (1-cyclohexyl-1-methylethyl
Peroxy-2-ethylhexanoate), own ester (the t-hexyl peroxy-2-ethyl of peroxidating -2 ethyl hexanoic acid the 3rd
Hexanoate), 4- methyl benzoyls peroxide (4-methylbenzoyl peroxide), peroxidating
- 2 ethyl hexanoic acid tributyl (t-butyl peroxy-2-ethylhexanoate), a toluyl groups benzene first
Acyl peroxide (m-toluoyl benzoyl peroxide), benzoyl peroxide (benzoyl
Peroxide), peroxidating isopropylformic acid. tributyl (t-butylperoxy isobutyrate), 1,1- pair (the
Tributyl peroxidating) 2- hexahydrotoluenes (1,1-bis (tert-butylperoxy)
- 2-methyl-cyclohexane), double (the 3rd hexyl the peroxidating) -3,3,5- trimethyl-cyclohexane (1,1-bis of 1,1-
(tert-butylperoxy) -3,3,5-trimethylcyclohexane), 1,1- double (the 3rd hexyl peroxidating)
Hexamethylene (11-bis (t-hexyl peroxy) cyclohexane), 1,1- are double (tributyl peroxidating)
- 3,3,5- trimethyl-cyclohexanes (1,1-bis (t-butylperoxy) -3,3,5-trimethylcyclohexane),
Double (tributyl peroxidating) Ketohexamethylene (1,1-bis (t-butylperoxy) cyclohexane) of 1,1-,
Double (the 4,4- dibutyl peroxidating cyclohexyl) propane (2,2-bis (4,4-di butylperoxy cyclohexyl) of 2,2-
Propane), double (tributyl peroxidating) cyclododecane (1,1-bis (t-butylperoxy) of 1,1-
Cyclododecane), the 3rd own ester (t-hexyl peroxy isopropyl of isopropyl peroxide single carbon acid
Monocarbonate), tributyl peroxidating maleic acid (t-butylperoxy maleic acid),
Peroxidating -3,5,5 Trimethylhexanoic acid tributyl (t-butyl peroxy-3,5,5-trimethylhexanoate),
Double (the toluene of peroxylauric acid tributyl (t-butylperoxy laurate), 2,5- dimethyl -2,5-
Formoxyl peroxidating) hexane (2,5-dimethyl-2,5-bis (m-toluoyl peroxy) hexane), mistake
Oxidation isopropyl single carbon acid tributyl (t-butylperoxy isopropyl monocarbonate), peroxide
Change -2- ethylhexyls single carbon acid tributyl (t-butylperoxy-2-ethylhexyl monocarbonate),
Double (the benzene first of the own ester (t-hexyl peroxybenzoate) of perbenzoic acid the 3rd, 2,5- dimethyl -2,5-
Acyl group peroxidating) hexane (2,5-dimethyl-2,5-bis (benzoyl peroxy) hexane), peroxidating
Double (tributyl peroxidating) butane (2,2-bis of acetic acid tributyl (t-butylperoxyacetate), 2,2-
(t-butylperoxy) butane), perbenzoic acid tributyl (t-butylperoxybenzoate),
Double (tributyl peroxidating) n-butyl pentanoates (n-butyl4,4-bis (t-butylperoxy) valerate) of 4,4-,
The tributyl (di-t-butylperoxyisophthalate) of peroxidating M-phthalic acid two-the, α, α '-bis- (three
Butyl peroxy) diisopropyl benzene (α, α '-bis (t-butylperoxy) diisopropylbenzene), mistake
Oxidation diisopropylbenzene (DIPB) (dicumylperoxide), 2,5- dimethyl -2,5- double (tributyl peroxidating) oneself
Alkane (2,5-dimethyl-2,5-di (t-butylperoxy) hexane), tributyl dicumyl peroxide
(t-butylcumylperoxide), to terpane hydroperoxides (p-menthane hydroperoxide),
Double (tributyl peroxidating) hexin (2,5-dimethyl-2,5-bis (t-butylperoxy) of 2,5- dimethyl -2,5-
Hexyne), diisopropyl benzene hydroperoxides (diisopropylbenzene hydroperoxide),
Tributyl TMS peroxide (t-butyl trimethylsilyl peroxide), 1,1,3,3- tetramethyls
Base butyl hydroperoxides (1,1,3,3-tetramethyl butyl hydroperoxide), isopropylbenzene hydrogen peroxide
Compound (cumene hydroperoxide), the 3rd hexyl hydroperoxides (t-hexyl hydroperoxide),
Tributyl hydroperoxides (t-butyl hydroperoxide), 2,3- dimethyl -2,3- diphenyl butanes
(2,3-dimethyl-2,3-diphenylbutane) or its combination.
But the present invention is not intended to limit the number of species of the initiator.In another embodiment, photochromic layer 160
In can include two or more initiator to different temperatures reaction so that photochromic layer 160 can be in response to
Different ambient temperature and change color.
Significantly, since the activity change speed of conversion zone 140 (can be for example oxidoreductase
Active rate of regression) be equal to photochromic layer 160 concentration change speed (can be for example conjugated polymer
Concentration change speed).Therefore, when the active rate of regression of the oxidoreductase in conversion zone 140 is for
When knowing (can such as by learn in experiment), the concentration change speed of the conjugated polymer in photochromic layer 160
Then can be defined with formula (2) by formula (1), its is as follows：
Wherein
K is the reaction rate constant of the photochromic layer 160, and it is defined by formula (2)；
T is the temperature under absolute scale；
A, b are experimental constant, and wherein a is the rate of change constant K and temperature T of photochromic layer 160
Intercept, b is the slopes of the rate of change constant K with temperature T of the photochromic layer 160；
C0For the original concentration of the photochromic layer 160；
CiFor the varied concentration to be presented of the photochromic layer 160, wherein the varied concentration is defined as reaction
Layer 140 loses varied concentration during original measurement function；
A is the reaction rate of conversion zone 140 or photochromic layer 160 at temperature T.
It should be noted that reaction rate constants of the K for photochromic layer 160, it can pass through described common
The concentration of conjugated polymer, monomer, sensitizer and initiator is adjusting.In other words, in the present embodiment,
Photochromic layer 160 can be controlled by the concentration of the conjugated polymer, monomer, sensitizer and initiator
Color change under specified temp and time.
However, the present invention is not intended to limit the color performance and excursion.As long as the discoloration of the present embodiment
The face that layer 160 is presented when losing original measurement function because of environmental factorss (can be for example ambient temperature)
Color is poor at a distance of the colour atla code of more than 1 with the color presented when just completing and producing (or just dispatching from the factory).
In one embodiment, photochromic layer 160 can lose original because of environmental factorss (can be for example ambient temperature)
The color presented during measurement function is presented the colour atla of more than 7 with the color presented when just completing and producing
Code is poor.
" the colour atla code " is " the color wildcard issued according to " Cai Tong companies " (Pantone Inc.)
Colour system is united”(PANTONE MATCHING) in specified color coding.Such as
Using Pantone Yellow 8pts, Pantone Warm Red 0.125pts and Pantone
The allotment achievement of Trans.Wt.56pts is the yellow of color logical color matching system colour atla code PMS 106C, and correlation is retouched
State in the notification number of U.S. Patent No. 5734800, patent name be " Six-color process system " in,
Here is incorporated into using as above-mentioned reference.And the colour atla code difference is " the logical color matching system of coloured silk" in
Coding it is poor, for example, PMS 106C and PMS 105C or PMS 107C are presented 1 colour atla code
Difference.
Fig. 2 is the loss of activity figure of the conversion zone of experimental example 1-2.
＜ tests ＞
In order to prove the realizability of the present invention, experimental example will be hereafter lifted with the present invention more particularly described below.
Although describing following experiment, in the case of insurmountability scope, can suitably change used
Material, its amount and ratio, process details and handling process etc..Therefore, should not be according to hereinafter described
Explanation of the experiment to restricting property of the present invention.
(experimental example 1)
In biochemical test piece configuration with conjugated polymer photochromic layer, wherein the biochemical test piece be to
Measure cholesterol.Then, the biochemical test piece is positioned over into temperature in the environment of 30 degree Celsius, continuously
Place 51 days, and observe the color change of photochromic layer.The color change of photochromic layer is as shown in Figure 2.
(experimental example 2)
In biochemical test piece configuration with conjugated polymer photochromic layer, wherein the biochemical test piece be to
Measure cholesterol.Then, the biochemical test piece is positioned over into temperature in the environment of 50 degree Celsius, continuously
Place 51 days, and observe the color change of photochromic layer.The color change of photochromic layer is as shown in Figure 2.
Shown by the result of Fig. 2, compared with experimental example 1, taken the photograph because the biochemical test piece of experimental example 2 is in
At a high temperature of 50 degree of family name, its oxidoreductase activity for being likely to result in experimental example 2 successively decreases, and causes experimental example
2 oxidoreductase loses original activity or forfeiture respond partially or completely.On the other hand,
The electron transfer instrumentality (e.g. potassium ferrocyanide) of experimental example 2 also can light or heat impact and lead
Structure change is caused, and the structure change is mostly irreversible reaction.Therefore, the biochemistry of experimental example 2
The activity of test piece just decreases below 90% (placing 8 days) from 100% (placing 0 day), and it falls into
Outside the allowed band (i.e. 90%) of sensing cholesterol.In other words, after placing 8 days, experimental example
2 biochemical test piece by permanent loss it is original measurement cholesterol function.
In addition, as shown in Fig. 2 the color of the photochromic layer of experimental example 1 from place 0 day to place 51 days all
For same color (PMS 3155C).And the color of the photochromic layer of experimental example 2 is from the color for placing 0 day
(PMS 3155C) changes to the placement color (PMS 2955C) of 21 days, and then changes to placement 51
It color (PMS 288C).Therefore, user is just available is pre-printed the comparison on test piece tank
Color, comparing the color of the photochromic layer of biochemical test piece can learn whether biochemical test piece is damaged.
In sum, the present invention is utilized and is at least configured on the region outside conversion zone and with conjugated polymers
The photochromic layer of thing, its color can change with the change of environmental factorss.And the color change of photochromic layer
Various words, numeral, figure, pattern, symbol etc. can be shown as.Therefore, user need not be by sample
Product are inserted reaction zone and just can learn whether the biochemical test piece is damaged, and it can not only reduce the consume of sample,
The risk of the detection error of biochemical test piece can also be reduced.
Finally it should be noted that：Various embodiments above is only illustrating technical scheme rather than right
Its restriction；Although being described in detail to the present invention with reference to foregoing embodiments, this area it is common
Technical staff should be understood：It still can modify to the technical scheme described in foregoing embodiments,
Either equivalent is carried out to which part or all technical characteristic；And these modifications or replacement, and
The scope of the essence disengaging various embodiments of the present invention technical scheme of appropriate technical solution is not made.
Claims (19)
1. a kind of biochemical test piece, it is characterised in that include：
Insulated substrate；
Electrode unit, on the insulated substrate；
First insulating spacer, on the electrode unit, and with opening, the opening expose portion
The electrode unit；
Conversion zone, in the opening；
Second insulating spacer, on first insulating spacer；And
Photochromic layer, is at least configured on the region outside the conversion zone, wherein the color of the photochromic layer
Change with the change of environmental factorss.
2. biochemical test piece according to claim 1, it is characterised in that the photochromic layer is configured in institute
State upside or downside, the upside of first insulating spacer or the downside of insulated substrate, or it is described second exhausted
The upside or downside of edge partition.
3. biochemical test piece according to claim 1, it is characterised in that the material bag of the photochromic layer
Include conjugated polymer.
4. biochemical test piece according to claim 3, it is characterised in that the conjugated polymer includes
Arene compound, non-aromatic compounds, anti-arene compound or its combination.
5. biochemical test piece according to claim 4, it is characterised in that the arene compound bag
Include polycyclic aromatic compound, phenyl compound, crystal violet derivant or its combination.
6. biochemical test piece according to claim 5, it is characterised in that the polycyclic aromatic hydrocarbon chemical combination
Thing include arylmethane compounds, fluoran compound, phenothiazine compounds, thio fluoran compound, cluck
In ton compound, spiropyran compounds .alpha.-5:6-benzopyran pyrazole compound, methylidyne compound, rhodamine
Amide compound, quinazoline compound, diaza cluck ton compound, dilactone compound or its combination.
7. biochemical test piece according to claim 5, it is characterised in that the phenyl compound includes
Cresol red derivant, thymol blue derivant, aniline yellow derivant, 2,4- dinitro benzene amphyls, bromine
Phenol indigo plant derivant, methyl yellow derivant, bromocresol green derivant, C.I. 13020. derivant, C.I. 14645. derive
Thing, bromocresol purple derivant, alizarin red derivant, a nitre phenol derivatives, o- cresol phthalide derivatives, phenol
Phthalide derivative, daphne phenol phthalide derivative, alizarin yellow derivant R, indigo carminum derivant, malachite green oxalate spread out
Biology, trisbromomethyl Benzosulfone derivatives, victoria blue B derivant, Victoria green G derivative,
Phthalocyanine blue derivant, phthalocyanine green derivant or its combination.
8. biochemical test piece according to claim 1, it is characterised in that the photochromic layer also includes single
Body, the monomer include noncrystalline polymer, chlorostyrene, ethylene, propylene, butylene, stretch isopentyl,
Vinyl acetate, propionate, vinyl benzoate, vinyl butyrate, acrylic acid methyl ester., propylene
Acetoacetic ester, alkene butyl acrylate, 2-EHA, phenyl acrylate, methyl methacrylate,
Ethyl methacrylate, butyl methacrylate, vinyl methylether, ethoxy ethylene, butyl vinyl ether,
Methyl vinyl ketone, acetylacetone,2,4-pentanedione, polyethylene and polypropylene, polyester resin, polyurethane tree
Fat, epoxy resin, silica resin, improved resin, paraffin or its combination.
9. biochemical test piece according to claim 1, it is characterised in that the photochromic layer also includes increasing
Sense agent, the sensitizer includes that unsaturated ketone, 1,2- derovatives, benzo radical derivative, fluorenes derive
Thing, naphthoquinone derivatives, anthraquinone derivative, dibenzo piperazine are muttered derivant, coumarin derivative, Hua Jingyan
Spend cyanines derivant, polymethine derivant, acridine derivatives, azepine benzene derivative, oxazines in biological, portion
Derivant, indoline derivative thing, azulene derivatives, derivatives of porphyrin, four benzene derivatives of porphyrin, three fragrant first
It is alkane derivatives, phthalocyanine derivates, wheel ene derivative, spiropyran derivatives, spirooxazine derivant, organic
Ruthenium misfit thing or its combination.
10. biochemical test piece according to claim 9, it is characterised in that the unsaturated ketone bag
Include chromocor derivative, Er Ya Benzyl acetone or its combination, 1, the 2- derovatives Bao Kuo Benzyl bases, Camphora
Quinone or its combination.
11. biochemical test pieces according to claim 1, it is characterised in that the formation of the photochromic layer
Method includes cladding process, ink jet printing method, twine print process or distributing print process.
12. biochemical test pieces according to claim 1, it is characterised in that the environmental factorss include
Light, temperature, humidity, oxygen content or its combination.
13. biochemical test pieces according to claim 1, it is characterised in that the color of the photochromic layer
Change and show word, numeral, figure, pattern, symbol or its combination.
14. biochemical test pieces according to claim 1, it is characterised in that the photochromic layer also includes
Initiator, the initiator includes organic peroxide.
15. biochemical test pieces according to claim 14, it is characterised in that the organic peroxide
Including isobutyl peroxide, α, α '-bis- (new capryl peroxidating) diisopropyl benzene, the peroxidating new last of the ten Heavenly stems
Sour cumyl ester, peroxy dicarbonate be double-n-propyl, peroxy dicarbonate double-the second butyl ester, neodecanoic acids
1,1,3,3- tetramethyl butyl esters, peroxy dicarbonate double (4- tributyl cyclohexyl) ester, the peroxidating new last of the ten Heavenly stems
Double -2- the ethoxy ethyl esters of sour 1- cyclohexyl -1- Methylethyls, peroxy dicarbonate, double (ethylhexyl peroxides
Change) two carbonic esters, the own ester of neodecanoic acid the 3rd, peroxy dicarbonate bi-methoxy butyl ester, double (3- methyl -3-
Methoxybutyl peroxidating) two carbonic esters, peroxidating neodecanoic acid tributyl, peroxidating pivalic acid the 3rd
Own ester, 3,5,5- trimethyl acetyl base peroxide, sim peroxides, lauroyl peroxide,
Stearyl peroxide, peroxidating -2 ethyl hexanoic acid 1,1,3,3- tetramethyl butyl esters, succinyl peroxide,
2,5- dimethyl -2,5- double (2- ethyl hexyl acyl groups) hexane, peroxidating -2 ethyl hexanoic acid 1- cyclohexyl -1- first
Base ethyl ester, the own ester of peroxidating -2 ethyl hexanoic acid the 3rd, 4- methyl benzoyl peroxide, peroxidating -2-
Thylhexoic acid tributyl, a toluyl benzoyl base peroxide, benzoyl peroxide,
Double (tributyl peroxidating) the 2- hexahydrotoluenes of peroxidating isopropylformic acid. tributyl, 1,1-, 1,1- double (the
Three hexyl peroxidating) -3,3,5- trimethyl-cyclohexanes, 1,1- double (the 3rd hexyl peroxidating) hexamethylene, 1,1-
Double (tributyl peroxidating) hexamethylenes of double (tributyl peroxidating) -3,3,5- trimethyl-cyclohexanes, 1,1-
Double (the 4,4- dibutyl peroxidating cyclohexyl) propane of ketone, 2,2-, double (tributyl peroxidating) rings of 1,1-
Dodecane, isopropyl peroxide single carbon the 3rd own ester of acid, tributyl peroxidating maleic acid, peroxide
Change -3,5,5 Trimethylhexanoic acid tributyl, peroxylauric acid tributyl, 2,5- dimethyl -2,5- pair (
Toluyl groups peroxidating) hexane, isopropyl peroxide single carbon acid tributyl, peroxidating -2- ethyl hexyls
Base single carbon acid tributyl, the own ester of perbenzoic acid the 3rd, double (the benzoyl mistakes of 2,5- dimethyl -2,5-
Oxidation) hexane, peracetic acid tributyl, 2,2- double (tributyl peroxidating) butane, peroxidating
Benzoic acid tert-butyl ester, 4,4- double (tributyl peroxidating) n-butyl pentanoate, peroxidating M-phthalic acids
Two-the tributyls, α, α '-bis- (tributyl peroxidating) diisopropyl benzene, cumyl peroxide, 2,5-
Double (tributyl peroxidating) hexane, the tributyl dicumyl peroxides of dimethyl -2,5-, to terpane
Hydroperoxides, 2,5- dimethyl -2,5- double (tributyl peroxidating) hexin, diisopropyl benzene hydrogen peroxides
It is compound, tributyl TMS peroxide, 1,1,3,3- tetramethyl butyl hydroperoxides, different
Propyl benzene hydroperoxides, the 3rd hexyl hydroperoxides, tributyl hydroperoxides, 2,3- dimethyl -2,3-
Diphenyl butane or its combination.
16. biochemical test pieces according to claim 1, it is characterised in that the activity of the conversion zone
Rate of change is equal to the concentration change speed of the photochromic layer.
17. biochemical test pieces according to claim 16, it is characterised in that the photochromic layer it is described
Concentration change speed is that its is as follows defining by formula (1) with formula (2)：
Wherein
K is the reaction rate constant of the photochromic layer, and it is defined by formula (2)；
T is the temperature under absolute scale；
A, b are experimental constant, and wherein a is the rate of change constant and the temperature of the photochromic layer
Intercept, b is the slope of the rate of change constant with the temperature of the photochromic layer；
C0For the original concentration of the photochromic layer；
CiFor the photochromic layer varied concentration to be presented, wherein the varied concentration is defined as the reaction
Layer loses varied concentration during original measurement function；
A is the reaction rate of the conversion zone or the photochromic layer at temperature T.
18. biochemical test pieces according to claim 17, it is characterised in that institute at a certain temperature
The reaction rate constant for stating photochromic layer is adjusted by the concentration of the compound in the photochromic layer.
19. biochemical test pieces according to claim 18, it is characterised in that the institute in the photochromic layer
Compound is stated including conjugated polymer, monomer, sensitizer, initiator or its combination.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510644752.6A CN106568711A (en) | 2015-10-08 | 2015-10-08 | Biochemical test piece |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510644752.6A CN106568711A (en) | 2015-10-08 | 2015-10-08 | Biochemical test piece |
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|---|---|
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|---|---|---|---|---|
| US6564620B1 (en) * | 1998-06-29 | 2003-05-20 | Conditions Incorporated | Visually indicating corrosion sensing |
| WO2005108968A1 (en) * | 2004-05-12 | 2005-11-17 | Matsushita Electric Industrial Co., Ltd. | Biosensor, container for biosensor, and biosensor measuring apparatus |
| CN102105125A (en) * | 2008-07-30 | 2011-06-22 | 金伯利-克拉克环球有限公司 | Absorbent products with wetness sensors |
| CN102362161A (en) * | 2009-01-22 | 2012-02-22 | 斯蒂茨丁荷兰聚合物学会 | Multifunctional optical sensor |
-
2015
- 2015-10-08 CN CN201510644752.6A patent/CN106568711A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6564620B1 (en) * | 1998-06-29 | 2003-05-20 | Conditions Incorporated | Visually indicating corrosion sensing |
| WO2005108968A1 (en) * | 2004-05-12 | 2005-11-17 | Matsushita Electric Industrial Co., Ltd. | Biosensor, container for biosensor, and biosensor measuring apparatus |
| CN102105125A (en) * | 2008-07-30 | 2011-06-22 | 金伯利-克拉克环球有限公司 | Absorbent products with wetness sensors |
| CN102362161A (en) * | 2009-01-22 | 2012-02-22 | 斯蒂茨丁荷兰聚合物学会 | Multifunctional optical sensor |
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Application publication date: 20170419 |