CN106526202B - A kind of closed pathogen detection cartridge - Google Patents
A kind of closed pathogen detection cartridge Download PDFInfo
- Publication number
- CN106526202B CN106526202B CN201611012226.9A CN201611012226A CN106526202B CN 106526202 B CN106526202 B CN 106526202B CN 201611012226 A CN201611012226 A CN 201611012226A CN 106526202 B CN106526202 B CN 106526202B
- Authority
- CN
- China
- Prior art keywords
- ring layer
- outer shroud
- layer
- sampler
- reagent trough
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
Abstract
The invention discloses a kind of closed pathogen detection cartridges, including outer shroud ring layer and inner ring ring layer, the inner ring ring layer is fitted in outer shroud ring layer, outer shroud ring layer is provided with insulating layer with inner collar interlayer, filling heat preservation cotton material inside insulating layer, outer shroud ring layer bottom is fixed on beam, inner ring ring layer inner wall side is provided with temperature sensor, temperature sensor is adjacent with reagent trough, reagent trough is opened on pedestal, reagent trough is corresponding with sampler, sampler is flexibly connected with sliding rail, sliding rail is arranged at the top of external toroidal ring layer, sampler side is connected with reeler, it is provided with knob on outer shroud ring layer side wall, knob is associated with reagent trough.The fast good airproof performance of detection speed of the present invention can detect various samples simultaneously and not will produce cross-infection, effectively avoid the problem that the pollution of pathogenic microorganism is infected with operator.
Description
Technical field
The present invention relates to detection cartridge technical field, specially a kind of closed pathogen detection cartridges.
Background technology
Detection of nucleic acids has been widely used in the fields such as biological medicine.Traditional detection of nucleic acids whole process (including
Nucleic acid extraction, PCR amplification detect) it needs to operate progress on different instruments, sample needs transfer multiple, this is greatly increased
Sample generates the probability of cross contamination, is especially polluted from the operating process for sample detection of uncapping after PCR amplification, and
Detection device lacks leakproofness and is also possible to that operating personnel is caused to infect.
Invention content
The purpose of the present invention is to provide a kind of closed pathogen detection cartridges, have the fast good airproof performance of detection speed
Advantage can detect various samples simultaneously and not will produce cross-infection, and the pollution and operator for solving pathogenic microorganism are infected
The problem of.
To achieve the above object, the present invention provides the following technical solutions：A kind of closed pathogen detection cartridge, including it is outer
Ring layer and inner ring ring layer, the inner ring ring layer are fitted in outer shroud ring layer, and outer shroud ring layer is provided with heat preservation with inner collar interlayer
Layer, insulating layer inside filling heat preservation cotton material, outer shroud ring layer bottom are fixed on beam, and inner ring ring layer inner wall side is provided with
Temperature sensor, temperature sensor is adjacent with reagent trough, and reagent trough is opened on pedestal, and reagent trough is corresponding with sampler,
Sampler is flexibly connected with sliding rail, and sliding rail is arranged at the top of external toroidal ring layer, and sampler side is connected with reeler, external toroidal ring
It is provided with knob on layer side wall, knob is associated with reagent trough.
Preferably, the sampler includes connector with movable, armature plate, centrifugal device and guide pipe, connector with movable
It is opened in armature center, armature is provided at both ends with interconnecting piece, and interconnecting piece is engaged with sliding rail, and guide pipe is fixedly connected on movement
Connector lower end is provided with centrifugal device in guide pipe, be oriented to pipe surface and wrap up outsourcing film, and outsourcing film is with from the outside to the core
Move mode is connected with reeler.
Preferably, it is equipped with nitrocellulose filter on the reagent trough baseplane, reagent trough is respectively arranged with from top to bottom
Well, test strip, absorbing membrane and air hole.
Preferably, described nitrocellulose filter one end is connected to connecting rod, and connecting rod is associated with knob.
Preferably, the sampler both sides open up observation window, and observation window is made of high plastic material thoroughly, observation window with
Inner collar interlayer is provided with sealing ring.
Compared with prior art, beneficial effects of the present invention are as follows：
1, the present invention is by being arranged mobile sampler and outsourcing film, can will be by with collection mode from the outside to the core
The portion collection of sample infection gets up, and the detection of multiple samples can be realized without will produce thereby using the same connector with movable
Cross-infection.
2, the present invention is conveniently replaceable nitrocellulose in the case where not opening kit by the way that connecting rod and knob is arranged
Film keeps sealing performance, avoids operating personnel's infection.
3, the present invention is by being arranged absorbing membrane and air hole so that sample climbing speed is accelerated, and detection time is shortened, and improves
The speed of detection.
Description of the drawings
Fig. 1 is schematic structural view of the invention；
Fig. 2 is structure of the invention reagent trough schematic diagram；
Fig. 3 is structure of the invention sampler schematic diagram.
In figure：1- outer shroud ring layers, 2- inner ring ring layers, 3- insulating layers, 4- observation windows, 5- reagent troughs, 51- wells, 52- inspections
Survey band, 53- absorbing membranes, 54- air holes, 55- nitrocellulose filters, 6- beams, 7- knobs, 8- sliding rails, 9- samplers,
91- connector with movable, 92- armature plates, 93- centrifugal devices, 94- guide pipes, 95- outsourcing films, 10-- reelers, 11- connecting rods,
12- temperature sensors, 13- pedestals.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
- 3 are please referred to Fig.1, the present invention provides a kind of technical solution：A kind of closed pathogen detection cartridge, including outer shroud
Ring layer 1 and inner ring ring layer 2, the inner ring ring layer 1 are fitted in outer shroud ring layer 2, are provided between outer shroud ring layer 2 and inner ring ring layer 1
Insulating layer 3,3 inside filling heat preservation cotton material of insulating layer, 2 bottom of outer shroud ring layer are fixed on beam 6,1 inner wall of inner ring ring layer
Side is provided with temperature sensor 12, and temperature sensor 12 is adjacent with reagent trough 5, and reagent trough 5 is opened on pedestal 13, reagent trough
5 is corresponding with sampler 9, and sampler 9 is flexibly connected with sliding rail 8, and the setting of sliding rail 8 is in 1 top of external toroidal ring layer, sampler
9 sides are connected with reeler 10, and knob 7 is provided on 1 side wall of outer shroud ring layer, and knob 7 is associated with reagent trough 5.
The sampler 9 includes connector with movable 91, armature plate 92, centrifugal device 93 and guide pipe 94, mobile connection
First 91 are opened in 92 center of armature, and armature 92 is provided at both ends with interconnecting piece, and interconnecting piece and sliding rail 8 are engaged, and guide pipe 94 is solid
Surely it is connected to 91 lower end of connector with movable, centrifugal device 93 is provided in guide pipe 94, outsourcing film is wrapped up on 94 surface of guide pipe
95, outsourcing film 94 is connected with move mode from the outside to the core with reeler 10, and shrinking outsourcing film 94 from the outside to the core can incite somebody to action
Got up by the portion collection that sample infects, thereby using the same connector with movable 91 can realize the detections of multiple samples without
Cross-infection is generated, while the setting of centrifugal device 93 makes sample solution ingredient uniform, the reagent trough is laid on 5 baseplane
There are nitrocellulose filter 55, reagent trough 5 to be respectively arranged with well 51, test strip 52, absorbing membrane 53 from top to bottom and breathe freely
Hole 54 accelerates sample climbing speed by absorbing membrane 53 and air hole 54, shortens detection time, the nitrocellulose filter 55 1
End is connected to connecting rod 11, and connecting rod is associated with knob 7, and nitrocellulose filter is conveniently replaceable in the case where not opening kit
55, sealing performance is kept, prevents operating personnel from infecting, 9 both sides of the sampler open up observation window 4, and observation window 4 is using high saturating
Plastic material is made, and is provided with sealing ring between observation window 4 and inner ring ring layer 2, good airproof performance and conducive to the internal detected status of observation.
Working procedure：When using this kind of closed pathogen detection cartridge, pathogen sample to be detected is filled by sampling
It sets 9 to be put into, is dropped in reagent trough 5 and is detected after mixing under the effect of centrifugal device 93, then is continuous to multiple samples
When detection, rotating scroll device 10, outsourcing film 95 is collected from the outside to the core along guide pipe 94, clean outsourcing film 94
It is attached on guide pipe 94 to wait for detecting next time, avoids the pollution of pathogenic microorganism, in temperature sensor 12 detects at any time
Portion's temperature regime avoids temperature from influencing testing result.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (4)
1. a kind of closed pathogen detection cartridge, including outer shroud ring layer（1）With inner ring ring layer（2）, it is characterised in that：In described
Ring layer（1）It is fitted in outer shroud ring layer（2）It is interior, outer shroud ring layer（2）With inner ring ring layer（1）Between be provided with insulating layer（3）, insulating layer
（3）Inside filling heat preservation cotton material, outer shroud ring layer（2）Bottom is fixed on beam（6）On, inner ring ring layer（1）Inner wall side is set
It is equipped with temperature sensor（12）, temperature sensor（12）With reagent trough（5）It is adjacent, reagent trough（5）It is opened in pedestal（13）On, examination
Agent slot（5）With sampler（9）It is corresponding, sampler（9）With sliding rail（8）Flexible connection, sliding rail（8）It is arranged in external toroidal ring layer
（1）Top, sampler（9）Side is connected with reeler（10）, outer shroud ring layer（1）Knob is provided on side wall（7）, knob
（7）With reagent trough（5）It is associated, the sampler（9）Including connector with movable（91）, armature plate（92）, centrifugal device
（93）And guide pipe（94）, connector with movable（91）It is opened in armature（92）Center, armature（92）It is provided at both ends with interconnecting piece, even
Socket part and sliding rail（8）It is engaged, guide pipe（94）It is fixedly connected on connector with movable（91）Lower end, guide pipe（94）Interior setting
There is centrifugal device（93）, guide pipe（94）Wrap up outsourcing film in surface（95）, outsourcing film（94）With mobile side from the outside to the core
Formula and reeler（10）It is connected.
2. a kind of closed pathogen detection cartridge according to claim 1, it is characterised in that：The reagent trough（5）Bottom
Nitrocellulose filter is equipped in plane（55）, reagent trough（5）It is respectively arranged with well from top to bottom（51）, test strip
（52）, absorbing membrane（53）And air hole（54）.
3. a kind of closed pathogen detection cartridge according to claim 2, it is characterised in that：The nitrocellulose filter
（55）One end is connected to connecting rod（11）, connecting rod and knob（7）It is associated.
4. a kind of closed pathogen detection cartridge according to claim 1, it is characterised in that：The sampler（9）
Both sides open up observation window（4）, observation window（4）It is made of high plastic material thoroughly, observation window（4）With inner ring ring layer（2）Between be arranged
There is sealing ring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611012226.9A CN106526202B (en) | 2016-11-17 | 2016-11-17 | A kind of closed pathogen detection cartridge |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611012226.9A CN106526202B (en) | 2016-11-17 | 2016-11-17 | A kind of closed pathogen detection cartridge |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106526202A CN106526202A (en) | 2017-03-22 |
| CN106526202B true CN106526202B (en) | 2018-07-13 |
Family
ID=58352162
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611012226.9A Active CN106526202B (en) | 2016-11-17 | 2016-11-17 | A kind of closed pathogen detection cartridge |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106526202B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5118474A (en) * | 1988-09-19 | 1992-06-02 | Vitaly Rogalsky | Laboratory pipet |
| CN104204229A (en) * | 2012-02-10 | 2014-12-10 | 株式会社百奥尼 | Apparatus and method for automatically analyzing biological samples |
| CN104818213A (en) * | 2015-05-14 | 2015-08-05 | 东南大学 | Sliding type closed pathogen detection card box |
| CN105021835A (en) * | 2015-07-28 | 2015-11-04 | 东南大学 | Magnetically driven biological sample treatment device and treatment method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6566145B2 (en) * | 2000-02-09 | 2003-05-20 | William E Brewer | Disposable pipette extraction |
-
2016
- 2016-11-17 CN CN201611012226.9A patent/CN106526202B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5118474A (en) * | 1988-09-19 | 1992-06-02 | Vitaly Rogalsky | Laboratory pipet |
| CN104204229A (en) * | 2012-02-10 | 2014-12-10 | 株式会社百奥尼 | Apparatus and method for automatically analyzing biological samples |
| CN104818213A (en) * | 2015-05-14 | 2015-08-05 | 东南大学 | Sliding type closed pathogen detection card box |
| CN105021835A (en) * | 2015-07-28 | 2015-11-04 | 东南大学 | Magnetically driven biological sample treatment device and treatment method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106526202A (en) | 2017-03-22 |
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