CN106442978A - Y-shaped two-channel fluorescence immunochromatography test strip and preparation method thereof - Google Patents
Y-shaped two-channel fluorescence immunochromatography test strip and preparation method thereof Download PDFInfo
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- CN106442978A CN106442978A CN201610877745.5A CN201610877745A CN106442978A CN 106442978 A CN106442978 A CN 106442978A CN 201610877745 A CN201610877745 A CN 201610877745A CN 106442978 A CN106442978 A CN 106442978A
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- pad
- nitrocellulose filter
- side arm
- arm
- test paper
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
Abstract
The invention discloses a Y-shaped two-channel fluorescence immunochromatography test strip and a preparation method thereof. The test strip comprises a Y-shaped substrate, the substrate comprises a vertical straight arm, and a first side arm and a second side arm are arranged on two sides of the top end of the straight arm; each of the first side arm and the second side arm is provided with a combination pad, a nitrocellulose membrane and an absorption pad from bottom to top, two ends of the nitrocellulose membrane are located at bottoms of the combination pad and the absorption pad, and the combination pad is labeled with a fluorescence compound; each nitrocellulose membrane is coated with a detection line antibody and a quality control line antibody from bottom to top; a sample pad is attached to the straight arm, the top of the sample pad is Y-shaped, and two top ends are located on top surfaces of lower ends of the first and second combination pads. With the adoption of the Y-shaped two-channel fluorescence immunochromatography test strip, synchronous and uniform distribution of a liquid sample can be realized, the separate reaction of chromatography in each channel makes reservation of optimal reaction conditions for different biomolecules possible, and support is finally provided for the multi-target detection of various immunochromatography test strips.
Description
Technical field
The invention belongs to technical field of immunoassay, particularly a kind of Y type dual pathways fluorescence immune chromatography test paper bar and its
Preparation method.
Background technology
Immunochromatography technique is that immunological technique is mutually tied by the one kind growing up the eighties in 20th century with chromatographic techniques
The rapid immunoassay method (being commonly called as test strips) closed, the features such as the method has directly perceived, quick, easy and simple to handle, low cost,
Qualitative, semiquantitative detection under the conditions of being widely deployed for non-lab environment in recent years.The application of immunochromatography technique
Field includes the detection in biomedicine of pathogen, medicine, hormone and metabolite, plant quarantine, veterinary, feedstuff/food
Safety and environmental pollution etc..Immuno-chromatographic test paper strip key component：Sample pad, pad, nitrocellulose filter and absorption pad.Inspection
With the nitrocellulose filter that is coated with T line and C line in advance for fixing chromatographic film during survey, solution Deca to be detected in sample pad,
By capillarity swimming forward, detect the receptor for target nothing to be measured on object and nitrocellulose filter in liquid simultaneously
(antigen or antibody) occurs specific immune response that immune complex is trapped at T line, and free labeled complex is then not
Forward, there is specific immune response with the receptor on C line in impacted continuation swimming, can strengthen colour developing or enzyme by label
Reaction obtains direct result, realizes detecting the purpose of thing to be detected.
The realization of immunochromatography Multiple detection at present generally adopts two methods：First, arranging a plurality of in test strips
Detection band；Second, a plurality of test strips parallel connection.The former is it is impossible to solve sterically hindered problem between a plurality of detection band, simultaneously
The conditional equilibrium point of multiple difference immunoreation is difficult to determine；The latter, could not solve the accurate of liquid in simple parallel connection
Distribution and synchronous chromatography problem, thus dosing accuracy is difficult to control.
Content of the invention
It is an object of the invention to provide a kind of Y type dual pathways fluorescence immune chromatography test paper bar, its structure simply, can be double
Individually reacted in passage, and realized the synchronization of fluid sample and evenly distributed.
It is a further object of the present invention to provide a kind of preparation method of Y type dual pathways fluorescence immune chromatography test paper bar, its work
Skill is simple, and the Y type dual pathways fluorescence immune chromatography test paper bar quality of preparation is good, and Detection results project.
For achieving the above object, the present invention takes technical scheme below：
A kind of Y type dual pathways fluorescence immune chromatography test paper bar, it includes substrate, and this substrate surface scribbles biologically inert and glues
Glue；This substrate is in Y type, has vertical straight-arm, this straight-arm top both sides upwardly extends and is provided with the first side arm and the second side arm；
This first side arm is sticked from the bottom to top successively the first pad, the first nitrocellulose filter and the first absorption pad,
This first nitrocellulose filter two ends is located at the bottom of this first pad and the first absorption pad, this first pad labelling respectively
There is the first fluorescent composition；This first nitrocellulose filter is coated with the first detection line antibody from the bottom to top and the first nature controlling line resists
Body；
This second side arm is sticked from the bottom to top successively the second pad, the second nitrocellulose filter and the second absorption pad,
This second nitrocellulose filter two ends is located at the bottom of this second pad and the second absorption pad, this second pad labelling respectively
There is the second fluorescent composition；This second nitrocellulose filter is coated with the second detection line antibody from the bottom to top and the second nature controlling line resists
Body；
This straight-arm is sticked and has sample pad, and this sample pad top is in Y type, and two tops are located at the first pad and second respectively
The top surface of pad lower end.
Further, described straight-arm bottom is rounded, and described sample pad bottom is also rounded.
Further, described sample pad and the first pad, the overlapping width of the second pad are 1mm.
Further, described first pad and the first nitrocellulose filter, described second pad and the second nitric acid are fine
The overlapping width of the plain film of dimension is 2mm.
Further, described first absorption pad and the first nitrocellulose filter, described second absorption pad and the second nitric acid are fine
The overlapping width of the plain film of dimension is 2mm.
Further, described first nitrocellulose filter and described second nitrocellulose filter are nano fibrous membrane.
Further, described first absorption pad and the second absorption pad are absorbent paper.
Further, described substrate is PVC, and the angle between described first side arm and the second side arm is 90 degree.
A kind of preparation method of above-mentioned Y type dual pathways fluorescence immune chromatography test paper bar, it comprises the following steps：
A. the first nitrocellulose filter is carried out respectively being coated, by of the first detection line antibody and the first nature controlling line antibody
Dinitrocellulose film carries out being coated of the second detection line antibody and the second nature controlling line antibody respectively；
B. will be standby in 37 DEG C of oven drying 2h to the first nitrocellulose filter and the second nitrocellulose filter；
C. by sample pad, the first pad and the second pad respectively with processing immersion bubble 30min, it is dried in 37 DEG C of baking ovens
Overnight；
D. the first pad after processing and the second pad are combined with the first fluorescent composition and the second fluorescence respectively
Substance markers, are dried 2h in 37 DEG C of baking ovens standby；
E. the first nitrocellulose filter is pasted in the middle part of described first side arm, the first pad is pasted on the first side arm
Bottom is simultaneously overlapping with this first nitrocellulose filter lower end；By the first absorption pad be pasted on the first side arm top and with this first nitre
Acid cellulose film upper end is overlapping；
F. the second nitrocellulose filter is pasted in the middle part of described second side arm, the second pad is pasted on described second
Side arm bottom is simultaneously overlapping with this second nitrocellulose filter lower end；By the second absorption pad be pasted on described second side arm top and with
This second nitrocellulose filter upper end is overlapping；
G. sample pad is pasted on straight-arm surface, and overlap is pressed on the first combination respectively to make two tops of this sample pad
Pad and the bottom of the second pad.
Further, the biologically inert adhesive surface of described substrate is pasted with protection sheet, and pasting, described first nitric acid is fine
Before the plain film of dimension, this protection sheet is removed；Described first nitrocellulose filter and described second nitrocellulose filter are electrostatic
Spinning nano fibrous membrane.
The invention has the beneficial effects as follows：Y type dual pathways fluorescence immune chromatography test paper bar of the present invention makes the same of fluid sample
Step evenly distributes and is achieved, and the independent reaction of the interior chromatography of each passage is most preferably reacted for different biological molecules reservation simultaneously
Condition provides possibility, is finally that the multiplex detection of all kinds of immuno-chromatographic test paper strips provides support.
Brief description
Fig. 1 is the structural representation of Y type dual pathways fluorescence immune chromatography test paper bar of the present invention.
Fig. 2 is three kinds of testing result schematic diagrams of Y type dual pathways fluorescence immune chromatography test paper bar of the present invention.
Specific embodiment
In order that technical scheme and beneficial effect become more apparent, with reference to embodiments with accompanying drawing, right
The present invention is further elaborated.It should be appreciated that specific embodiment described herein is only in order to explain the present invention, and
It is not used in the restriction present invention.
As shown in figure 1, the present invention provides a kind of Y type dual pathways fluorescence immune chromatography test paper bar, it includes substrate 1, for example
PVC board, this substrate 1 surface scribbles biologically inert viscose glue.This substrate 1 is in Y type, has vertical straight-arm 11, this straight-arm 11 top
Both sides upwardly extend and are provided with the first side arm 12 and the second side arm 13.Angle between this first side arm 12 and the second side arm 13 is 90
Degree.
This first side arm 12 is sticked from the bottom to top successively the first pad 2, the first nitrocellulose filter 3 and the first absorption
Pad 4, this first nitrocellulose filter 3 two ends is located at the bottom of this first pad 2 and the first absorption pad 4 respectively, this first knot
Close pad 2 and be marked with the first fluorescent composition.This first nitrocellulose filter 3 is coated with the first detection line antibody and from the bottom to top
One nature controlling line antibody.
This second side arm 13 is sticked from the bottom to top successively the second pad 5, the second nitrocellulose filter 6 and the second absorption
Pad 7, this second nitrocellulose filter 6 two ends is located at the bottom of this second pad 5 and the second absorption pad 7 respectively, this second knot
Close pad 5 and be marked with the second fluorescent composition.This second nitrocellulose filter 6 is coated with the second detection line antibody and from the bottom to top
Two nature controlling line antibody.Preferably, this first absorption pad 4 and the second absorption pad 7 can be absorbent paper, in addition, this first nitric acid is fine
The plain film 3 of dimension and this second nitrocellulose filter 6 are Electrospun nano-fibers film so that the film parameters of chromatographic film can be adjusted on demand
Whole, detection sensitivity can be improved, there is good use value.
This straight-arm 11 is sticked and has sample pad 8, for accepting and filtering detection sample.This sample pad 8 top is in Y type, two
Top is located at the top surface of the first pad 2 and the second pad 5 lower end respectively.This straight-arm 11 bottom is rounded, this sample pad 8 bottom
Portion is also rounded, for storage release detection liquid.
The present invention is because the uniformity of sample pad 8 is so that the synchronization of fluid sample evenly distributes and is achieved, and simultaneously each
In passage, the independent reaction of chromatography provides possibility for different biological molecules reservation most preferably reaction condition again, is finally all kinds of exempting from
The multiplex detection of epidemic disease chromatograph test strip provides support.
Specifically, the overlapping width of this sample pad 8 and the first pad 2, the second pad 5 is 1mm.This first knot
Close pad 2 and the overlapping width of the first nitrocellulose filter 3, this second pad 5 and the second nitrocellulose filter 6 is 2mm.Should
First absorption pad 4 is with the first nitrocellulose filter 3, this second absorption pad 7 and the overlapping width of the second nitrocellulose filter 6
2mm.
The present invention also provides a kind of preparation method of above-mentioned Y type dual pathways fluorescence immune chromatography test paper bar, under it includes
Row step：
First nitrocellulose filter 3 is carried out being coated of the first detection line antibody and the first nature controlling line antibody respectively, by
Dinitrocellulose film 6 carries out being coated of the second detection line antibody and the second nature controlling line antibody respectively；
Will be standby in 37 DEG C of oven drying 2h to the first nitrocellulose filter 3 and the second nitrocellulose filter 6；
By sample pad 8, the first pad 2 and the second pad 5 respectively with processing immersion bubble 30min, dry in 37 DEG C of baking ovens
Dry overnight；
The first pad 2 after processing and the second pad 5 are combined with the first fluorescent composition and the second fluorescence respectively
Substance markers, are dried 2h in 37 DEG C of baking ovens standby；
The biologically inert adhesive surface of this substrate 1 is pasted with protection sheet, before pasting this first nitrocellulose filter 3,
This protection sheet is removed；
First nitrocellulose filter 3 is pasted in the middle part of this first side arm 12, the first pad 2 is pasted on the first side arm
12 bottoms are simultaneously overlapping with this first nitrocellulose filter 3 lower end；By the first absorption pad 4 be pasted on the first side arm 12 top and with this
First nitrocellulose filter 3 upper end is overlapping；
Second nitrocellulose filter 6 is pasted in the middle part of this second side arm 13, the second pad 5 is pasted on this second side
Arm 13 bottom is simultaneously overlapping with this second nitrocellulose filter 6 lower end；Second absorption pad 7 is pasted on this second side arm 13 top simultaneously
Overlapping with this second nitrocellulose filter 6 upper end；
Sample pad 8 is pasted on straight-arm 11 surface, and overlap is pressed on the first knot respectively to make two tops of this sample pad 8
Close the bottom of pad 2 and the second pad 5.
Y type dual pathways fluorescence immune chromatography test paper bar preparation method of the present invention, process is simple, no cross influence,
It is suitable for large-scale commercial production.
The present invention can realize twin-channel function in test strips by the design of Y type so that detection solution can be all
In the even independent detection zone being assigned to Y type dual pathways fluorescence immune chromatography test paper bar two wall, it is possible to achieve liquid in the dual pathways
The uniform, synchronous of sample is distributed, and can solve the homogeneity of the corresponding each component of test strips synchronously required for chromatography for the dual pathways
Problem.
Before examinations, for target detection thing, develop each self-corresponding single target respectively and be based on fluorescence nano grain
The immunochromatography device of son, obtain the corresponding inside of each Autonomous test as wide in range as possible, between as parallel as possible chromatography reactant
System；The reaction system of two detections is mutually balanced fusion, makes two detections anti-using dual pathways immunochromatography device simultaneously
Should synchronous uniformly carry out, thus on the premise of ensureing respective sensitivity and specificity, realizing dual pathways target synchronous detecting.
The present invention can also carry out the design of dual pathways immunochromatography interpretoscope：By two-dimensional movement platform, intelligent positioning system
System, activating system, reception system, the screening of control system and optimization, combined with intelligent mathematics algorithm, realize being based on fluorescence nano grain
On the dual pathways immunochromatography device of son, specific immunity detection signal automatically stripping and extracting.
The specific operation process of Y type dual pathways fluorescence immune chromatography test paper bar preparation method of the present invention is：Tear off on substrate
Viscose glue protection ply of paper expose biologically inert viscose glue, be coated the first detection line antibody and the first matter taking the first side arm 12 as a example
First nitrocellulose filter 3 of control line antibody is affixed at a certain distance from straight-arm 11, and the first fluorescent labeling is combined substance markers
First pad 2 is affixed at straight-arm suitable distance, then sample pad 8 and the first absorption pad 4 are affixed on the first nitrocellulose filter 3
Two ends.During detection sample, on the planchet of the sample pad 8 of the test strips that 200 μ l measuring samples solution additions are assembled, sample is molten
Liquid swimming forward under capillary action, when the first pad 2 thereon be dried first fluorescent labeling complex redissolve and with
Sample solution migrates forward, specifically binds in specific antigen or antibody zone position, fluorescent grain is in respective regions
Assemble and strengthen colour developing, can with the naked eye be observed under uviol lamp it is also possible to special-purpose assorted instrument carries out reading, thus realizing determining
Property and quantitative detection.As shown in Fig. 2 taking sandwich reaction as a example testing result is described：Y type dual pathways fluorescence immune chromatography tries
One end positive findingses A of paper slip assumes two lines, i.e. detection line 9 and nature controlling line 10；Only a line, i.e. matter in negative findings B
Control line 10；If no C in lines, illustrate that detection time is too short or test strips lost efficacy.
The above, be only presently preferred embodiments of the present invention, not the structure of the present invention is made any pro forma
Limit.Any simple modification, equivalent variations and modification that every technical spirit according to the present invention is made to above example, all
Still fall within the range of technical scheme.
Claims (10)
1. it is characterised in that it includes substrate, this substrate surface scribbles life to a kind of Y type dual pathways fluorescence immune chromatography test paper bar
Thing inertia viscose glue；This substrate is in Y type, has vertical straight-arm, and this straight-arm top both sides upwardly extends and is provided with the first side arm and the
Two side arms；
This first side arm is sticked from the bottom to top successively the first pad, the first nitrocellulose filter and the first absorption pad, and this
One nitrocellulose filter two ends are located at the bottom of this first pad and the first absorption pad respectively, and this first pad is marked with the
One fluorescent composition；This first nitrocellulose filter is coated with the first detection line antibody and the first nature controlling line antibody from the bottom to top；
This second side arm is sticked from the bottom to top successively the second pad, the second nitrocellulose filter and the second absorption pad, and this
Dinitrocellulose film two ends are located at the bottom of this second pad and the second absorption pad respectively, and this second pad is marked with the
Two fluorescent compositions；This second nitrocellulose filter is coated with the second detection line antibody and the second nature controlling line antibody from the bottom to top；
This straight-arm is sticked and has sample pad, and this sample pad top is in Y type, and two tops are located at the first pad and the second combination respectively
The top surface of pad lower end.
2. Y type dual pathways fluorescence immune chromatography test paper bar according to claim 1 it is characterised in that：Described straight-arm bottom
Rounded, described sample pad bottom is also rounded.
3. Y type dual pathways fluorescence immune chromatography test paper bar according to claim 1 and 2 it is characterised in that：Described sample pad
It is 1mm with the overlapping width of the first pad, the second pad.
4. Y type dual pathways fluorescence immune chromatography test paper bar according to claim 3 it is characterised in that：Described first combination
Pad is 2mm with the overlapping width of the first nitrocellulose filter, described second pad and the second nitrocellulose filter.
5. Y type dual pathways fluorescence immune chromatography test paper bar according to claim 4 it is characterised in that：Described first absorption
Pad is 2mm with the overlapping width of the first nitrocellulose filter, described second absorption pad and the second nitrocellulose filter.
6. Y type dual pathways fluorescence immune chromatography test paper bar according to claim 1 it is characterised in that：Described first nitric acid
Cellulose membrane and described second nitrocellulose filter are nano fibrous membrane.
7. Y type dual pathways fluorescence immune chromatography test paper bar according to claim 1 it is characterised in that：Described first absorption
Pad and the second absorption pad are absorbent paper.
8. Y type dual pathways fluorescence immune chromatography test paper bar according to claim 1 it is characterised in that：Described substrate is
PVC, the angle between described first side arm and the second side arm is 90 degree.
9. the preparation method of the Y type dual pathways fluorescence immune chromatography test paper bar any one of a kind of claim 1 to 8, its
It is characterised by, it comprises the following steps：
A. the first nitrocellulose filter is carried out respectively being coated, by the second nitre of the first detection line antibody and the first nature controlling line antibody
Acid cellulose film carries out being coated of the second detection line antibody and the second nature controlling line antibody respectively；
B. will be standby in 37 DEG C of oven drying 2h to the first nitrocellulose filter and the second nitrocellulose filter；
C. by sample pad, the first pad and the second pad respectively with processing immersion bubble 30min, dried in 37 DEG C of baking ovens
Night；
D. by the first pad after processing and the second pad respectively with the first fluorescent composition and the second fluorescent composition mark
Note, is dried 2h in 37 DEG C of baking ovens standby；
E. the first nitrocellulose filter is pasted in the middle part of described first side arm, the first pad is pasted on the first side arm bottom
And it is overlapping with this first nitrocellulose filter lower end；First absorption pad is pasted on the first side arm top fine with this first nitric acid
The plain film upper end of dimension is overlapping；
F. the second nitrocellulose filter is pasted in the middle part of described second side arm, the second pad is pasted on described second side arm
Bottom is simultaneously overlapping with this second nitrocellulose filter lower end；By the second absorption pad be pasted on described second side arm top and with this
Dinitrocellulose film upper end is overlapping；
G. sample pad is pasted on straight-arm surface, and make two tops of this sample pad respectively overlap be pressed on the first pad and
The bottom of the second pad.
10. the preparation method of Y type dual pathways fluorescence immune chromatography test paper bar according to claim 9 is it is characterised in that institute
The biologically inert adhesive surface stating substrate is pasted with protection sheet, before pasting described first nitrocellulose filter, this is protected
Paper removes；Described first nitrocellulose filter and described second nitrocellulose filter are Electrospun nano-fibers film.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107290540A (en) * | 2017-06-22 | 2017-10-24 | 中国农业大学 | Escherichia coli O 157:H7 and the common Test paper of salmonella typhimurium and preparation method and application |
| CN109725150A (en) * | 2018-12-20 | 2019-05-07 | 江南大学 | A kind of immune joint inspection test strips with sample distribution pad |
| CN110736831A (en) * | 2019-11-28 | 2020-01-31 | 河南大学 | multidirectional combined test strip |
| WO2020143220A1 (en) * | 2019-01-11 | 2020-07-16 | 广州万孚生物技术股份有限公司 | Immunochromatographic assay device |
| CN113009137A (en) * | 2021-03-12 | 2021-06-22 | 齐鲁工业大学 | Test strip, detection test paper box and application thereof |
| EP3910333A4 (en) * | 2019-01-11 | 2022-04-13 | Guangzhou Wondfo Biotech. Co., Ltd. | Sample loading bottom plate and immunochromatography detection apparatus containing sample loading bottom plate |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040166021A1 (en) * | 2003-02-20 | 2004-08-26 | Hubert Hsu | Multiplex test kit |
| WO2009090861A1 (en) * | 2008-01-16 | 2009-07-23 | Panasonic Corporation | Method for analyzing sample solution and apparatus for analyzing sample solution |
| CN201555849U (en) * | 2009-11-18 | 2010-08-18 | 吉林大学第一医院 | Disc type collaurum immunochromatography joint detection plate |
| CN101949930A (en) * | 2010-09-13 | 2011-01-19 | 无锡市金坤生物工程有限公司 | Disc for fast detecting mycotoxin indexes |
| CN202583199U (en) * | 2011-12-13 | 2012-12-05 | 中华人民共和国北京出入境检验检疫局 | Synchronous detection card structure |
| CN202870092U (en) * | 2012-10-25 | 2013-04-10 | 武汉伊艾博科技有限公司 | Parallel detecting plate of test strip |
| CN104428676A (en) * | 2012-07-19 | 2015-03-18 | 松下知识产权经营株式会社 | In vitro diagnostic tool, membrane for in vitro diagnostic tool, and methods for manufacturing same |
| CN204347031U (en) * | 2015-01-19 | 2015-05-20 | 郑州大学 | The detection dish of 9 kinds of objectionable impuritiess in feed is detected fast the while of a kind of |
| CN205538992U (en) * | 2016-03-30 | 2016-08-31 | 珠海华澳生物科技有限公司 | Test paper dish and test paper tray salver |
-
2016
- 2016-10-08 CN CN201610877745.5A patent/CN106442978A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040166021A1 (en) * | 2003-02-20 | 2004-08-26 | Hubert Hsu | Multiplex test kit |
| WO2009090861A1 (en) * | 2008-01-16 | 2009-07-23 | Panasonic Corporation | Method for analyzing sample solution and apparatus for analyzing sample solution |
| CN201555849U (en) * | 2009-11-18 | 2010-08-18 | 吉林大学第一医院 | Disc type collaurum immunochromatography joint detection plate |
| CN101949930A (en) * | 2010-09-13 | 2011-01-19 | 无锡市金坤生物工程有限公司 | Disc for fast detecting mycotoxin indexes |
| CN202583199U (en) * | 2011-12-13 | 2012-12-05 | 中华人民共和国北京出入境检验检疫局 | Synchronous detection card structure |
| CN104428676A (en) * | 2012-07-19 | 2015-03-18 | 松下知识产权经营株式会社 | In vitro diagnostic tool, membrane for in vitro diagnostic tool, and methods for manufacturing same |
| CN202870092U (en) * | 2012-10-25 | 2013-04-10 | 武汉伊艾博科技有限公司 | Parallel detecting plate of test strip |
| CN204347031U (en) * | 2015-01-19 | 2015-05-20 | 郑州大学 | The detection dish of 9 kinds of objectionable impuritiess in feed is detected fast the while of a kind of |
| CN205538992U (en) * | 2016-03-30 | 2016-08-31 | 珠海华澳生物科技有限公司 | Test paper dish and test paper tray salver |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107290540A (en) * | 2017-06-22 | 2017-10-24 | 中国农业大学 | Escherichia coli O 157:H7 and the common Test paper of salmonella typhimurium and preparation method and application |
| CN109725150A (en) * | 2018-12-20 | 2019-05-07 | 江南大学 | A kind of immune joint inspection test strips with sample distribution pad |
| WO2020143220A1 (en) * | 2019-01-11 | 2020-07-16 | 广州万孚生物技术股份有限公司 | Immunochromatographic assay device |
| EP3910333A4 (en) * | 2019-01-11 | 2022-04-13 | Guangzhou Wondfo Biotech. Co., Ltd. | Sample loading bottom plate and immunochromatography detection apparatus containing sample loading bottom plate |
| CN110736831A (en) * | 2019-11-28 | 2020-01-31 | 河南大学 | multidirectional combined test strip |
| CN113009137A (en) * | 2021-03-12 | 2021-06-22 | 齐鲁工业大学 | Test strip, detection test paper box and application thereof |
| CN113009137B (en) * | 2021-03-12 | 2023-07-18 | 齐鲁工业大学 | Test strip, test paper box and application thereof |
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Application publication date: 20170222 |