CN106434342A - Cell culture dish - Google Patents
Cell culture dish Download PDFInfo
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- CN106434342A CN106434342A CN201610845234.5A CN201610845234A CN106434342A CN 106434342 A CN106434342 A CN 106434342A CN 201610845234 A CN201610845234 A CN 201610845234A CN 106434342 A CN106434342 A CN 106434342A
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- culture dish
- camera lens
- tissue culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/08—Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a cell culture dish, and belongs to the field of biological materials. The cell culture dish comprises a dish box and a lens which are detachably connected through a connecting pieces, the bottom of the dish box is made from a high-transparency material, the thickness of the bottom of the dish box is smaller than 0.7 mm, and the lens is connected with a zoom adjustment system. The cell culture dish has the advantages that the requirement of high-power microscopy can be met, cell culture and observation are integrated, culture and observation can be conducted simultaneously, and the phenomenon that the cell function is affected due to the fact that a cell body makes contact with air can be avoided; meanwhile, when observation is conducted, a culture solution can be sucked out conveniently, and the influence of observation is reduced.
Description
Technical field
The present invention relates to a kind of culture dish, particularly a kind of Tissue Culture Dish.
Background technology
In view of extensive application of the microscopy in Basic of Biology research and medical industry, various living cells show
Micro mirror imaging technique becomes the important tool in biomedical research.Go for high-quality, high-resolution living cells microscope
Image, the parameter such as the consumptive material thickness of its cultured cells, coefficient of refraction is extremely important, especially goes for high-resolution living cells
Micro-image, it is often necessary to first-class using the microscope of high power, the now requirement to Tissue Culture Dish base material, especially thick
The requirement of degree and coefficient of refraction is very harsh.Cell culture consumptive material common at present, the relevant parameter such as thickness of its base material
All be unsatisfactory for the demand of high-resolution imaging so that carry out high-resolution living cells imaging process become extremely complex；It is frequently necessary to
After cell is first cultivated, then cell is moved on microscope slide, then microscope slide is fixed in imaging systems, allow fiber camera lens
Can be imaged directly facing microscope slide, in this course, may be revealed along with culture fluid, microscope slide may be exposed to
In the air time course and affect cell function, will cyton move into microscope slide during may affect cell function etc. lack
Point.
Content of the invention
The goal of the invention of the present invention is：For above-mentioned problem, provide a kind of disclosure satisfy that high power micro- will
Ask, integrate cultured cells and observe, can be observed while culture, it is to avoid cyton ingress of air affects cell
Function, while can facilitate in observation suctioning out culture fluid, reduces the Tissue Culture Dish of observation impact.
The technical solution used in the present invention is as follows：
A kind of Tissue Culture Dish of the present invention, including ware box and camera lens, the ware box and camera lens are detachably connected by connector, institute
State ware cassette bottom portion to be made up of high light transmissive material, the thickness in the ware cassette bottom portion is less than 0.7mm, and the camera lens is connected with zooming adjustment
System.
Due to employing technique scheme, by connector, ware box and camera lens are detachably connected, you can needing to see
During survey, ware box is connected with camera lens by connector, ware cassette bottom portion is made up of high light transmissive material, it is seen that the transmitance of light is
98.43%, while disclosure satisfy that the toughness in incubation to vessel requirement, camera lens is connected with zooming adjustment system can be normal
Be observed.
A kind of Tissue Culture Dish of the present invention, the ware box surface is evenly equipped with some culture tanks, and the culture tank is rectangular
Array arrangement, the culture tank is rounded, and the horizontal and vertical upper two adjacent culture tank is all by connecting canal connection, the company
Connect the depth of the depth less than culture tank of canal.
As technique scheme is employed, cell culture is carried out in culture tank, connection canal can will ensure culture fluid
Flowing in whole cultivation region, meanwhile, if culture fluid is impacted to observation, in fixed point, culture fluid can all be inhaled
/ injection is played, reduces the destruction during picking up/injecting culture fluid to cell.
A kind of Tissue Culture Dish of the present invention, the culture tank positioned at array edges is transversely connected with connection with longitudinal direction
Canal, the spacing >=0.02cm between two culture tank, the depth of the culture tank be 0.47cm, the connection canal depth≤
0.21cm.
Due to employing technique scheme, in edge fixed point batch, culture fluid can be picked up/be injected, will not be to training
Damage in cell in foster groove so that observed result is more accurate, connect the depth of the depth much smaller than culture tank of canal, energy
During enough avoiding cell culture, cell overflows and is grown in connecting in canal, impact observation.
A kind of Tissue Culture Dish of the present invention, is connected with gain piece between the connector and ware box, the gain piece includes
The photic zone one being linked in sequence from top to bottom, gain layer and photic zone two, the thickness of the photic zone one and photic zone two is
0.08cm, the thickness of the gain layer is 1.22mm, and the photic zone one and photic zone two are made by high light transmissive material.
Due to employing technique scheme, gain layer can increase the accuracy of observation to carrying out gain with regard to light, full
The observation condition that sufficient High Accuracy Observation needs.
A kind of Tissue Culture Dish of the present invention, is provided with stepped hole in the middle part of the connector, the stepped hole include hole and
Lower opening, the diameter in the upper hole is equal to the diameter of gain piece, and the diameter of the lower opening is equal to the diameter of camera lens
Size；The upper in the hole wall is provided with profile of tooth buckling groove, and the gain piece bottom is provided with clamping engagement groove；The gain piece and ware
Box is flexibly connected by screw thread；The camera lens is flexibly connected with lower opening by screw thread.
As technique scheme is employed, the distance of distance of camera lens ware box is substantially reduced, and the effect of gain piece is obtained
Farthest play, meanwhile, in observation, the space between ware box, gain piece and camera lens is less, substantially reduce three it
Between air flow, impact of the air to cytoactive is down to minimum.
A kind of Tissue Culture Dish of the present invention, the hole depth of the lower opening is equal to the thickness of camera lens, the hole depth in the upper hole etc.
Thickness in gain piece.
As technique scheme, connector and the compatible degree height of camera lens and gain piece is employed, maximum journey is ensure that
The distance between the reduction ware box of degree and camera lens.
A kind of Tissue Culture Dish of the present invention, the high light transmissive material by 37 parts of polymethyl methacrylates of mass parts, 59
Part polyamine
13 parts of methacrylic acids, 40 parts of SnO2With 18 parts of Sm2O3Composition, the polyamine n：M is 5：7, the SnO2And Sm2O3Particle diameter little
In 700nm.
Due to employing technique scheme, the light transmission of material is fabulous, it is seen that the transmitance of light can reach 98.43%,
In observation, although the stop having compared with multilamellar, but optical attenuation degree is low, and therefore, observation effect is good, disclosure satisfy that high accuracy is seen
The demand of survey；Material can prepare the light transmissive material of thinner thickness with good ductility before hardening is dry.
A kind of Tissue Culture Dish of the present invention, the gain layer is by 59 parts of polyamine of mass parts
13 parts of methacrylic acids, 5 parts of InGaAs, 0.7 part of Graphene and 7 parts of YVO4Composition, the polyamine n：M is 5：7.
Due to employing technique scheme, gain layer has certain gain effect to visible ray, can stimulated radiation,
The intensity of a small amount of amplification visible ray, can make up the light intensity decays that light transmissive material brings.
In sum, due to employing technique scheme, the invention has the beneficial effects as follows：
1st, the micro- requirement of high power is disclosure satisfy that, is integrated cultured cells and observe, can be observed while culture,
Cyton ingress of air impact cell function being avoided, while can facilitate in observation suctioning out culture fluid, reducing observation impact.
2nd, the transmitance of visible ray is 98.43%, to disclosure satisfy that the toughness to vessel requirement in incubation, to visible
Light has certain gain effect, can stimulated radiation, the intensity of a small amount of amplification visible ray, light transmissive material can be made up and brought
Light intensity decays, meet the demand of High Accuracy Observation.
Description of the drawings
Fig. 1 is a kind of Tissue Culture Dish structural representation.
In figure labelling：2 is ware box, and 3 is gain piece, and 4 is connector, and 5 is camera lens.
Specific embodiment
Below in conjunction with the accompanying drawings, the present invention is described in detail.
In order that the purpose of invention, technical scheme and advantage become more apparent, below in conjunction with drawings and Examples, to this
Invention is further elaborated.It should be appreciated that specific embodiment described herein is only in order to explain the present invention, not
For limiting the present invention.
Embodiment 1
As shown in figure 1, a kind of Tissue Culture Dish, including ware box 2 and camera lens 5, ware box 2 and camera lens 5 are detachably connected by connector 4
Connect, 2 bottom of ware box is made up of high light transmissive material, the thickness of 2 bottom of ware box is less than 0.7mm, and camera lens 5 is connected with zooming adjustment system
System.2 surface of ware box is evenly equipped with some culture tanks, and culture tank rectangular array is arranged, and culture tank is rounded, and horizontal and vertical upper two
Adjacent culture tank connects the depth of the depth less than culture tank of canal all by connecting canal connection.Culture tank positioned at array edges
Transversely with longitudinal direction, connection canal is connected with, the spacing >=0.02cm between two culture tanks, the depth of culture tank is 0.47cm, connects canal
Depth≤0.21cm.Gain piece 3 is connected between connector 4 and ware box 2, and it is saturating that gain piece 3 includes to be linked in sequence from top to bottom
Photosphere one, gain layer and photic zone two, the thickness of photic zone one and photic zone two is 0.08cm, and the thickness of gain layer is
1.22mm, photic zone one and photic zone two are made by high light transmissive material.4 middle part of connector is provided with stepped hole, and stepped hole includes
Upper hole and lower opening, the diameter in upper hole is equal to the diameter of gain piece 3, and the diameter of lower opening is equal to the diameter of camera lens 5
Size；Upper in the hole wall is provided with profile of tooth buckling groove, and 3 bottom of gain piece is provided with clamping engagement groove；Gain piece 3 and ware box 2 pass through spiral shell
Stricture of vagina is flexibly connected；Camera lens 5 is flexibly connected with lower opening by screw thread.The hole depth of lower opening is equal to the thickness of camera lens 5, the hole depth in upper hole etc.
Thickness in gain piece 3.
High light transmissive material is by 37 parts of polymethyl methacrylates of mass parts, 59 parts of polyamine
13 parts of methacrylic acids, 40 parts of SnO2With 18 parts of Sm2O3Composition, polyamine n：M is 5：7, SnO2And Sm2O3Particle diameter be less than 700nm.
Gain layer is by 59 parts of polyamine of mass parts
13 parts of methacrylic acids, 5 parts of InGaAs, 0.7 part of Graphene and 7 parts of YVO4Composition, polyamine n：M is 5：7.
Embodiment 2
Polyamine
And the synthetic method of high light transmissive material is as follows：
4 '-diaminodiphenyl ether and with 2,5- bis- (4- aminobenzene) pyrimidine according to mol ratio 5：There-necked flask is added after 7 mix homogeneously
In, in there-necked flask, add appropriate DMAC system solid content to be caused for 8% ~ 12%, be passed through excessive nitrogen as shielding gas, heat
5 ~ 8h of backflow, after question response thing is complete, obtains yellow solution, according to both summations and 4,4 '-hexafluoroisopropyli,ene-neighbour's benzene
Dioctyl phthalate mol ratio 1：1.005 add 4 in there-necked flask, and 4 '-hexafluoroisopropyli,ene-phthalic acid continues to be passed through excessive
Nitrogen is reacted 5h at room temperature, obtains polyamine glue as shielding gas.
Polymethyl methacrylate, methacrylic acid, nano SnO is added in glue according to mass ratio2With nanometer Sm2O3
After 2 ~ 3h is stirred at room temperature, mixed glue solution casting film-forming on totally smooth glass plate is taken, is existed after 2h is dry at 80 DEG C again
8 ~ 12h is dried under vacuum condition, the high light transmission film material of water white transparency.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and
Any modification, equivalent and improvement for being made within principle etc., should be included within the scope of the present invention.
Claims (8)
1. a kind of Tissue Culture Dish, it is characterised in that：Including ware box（2）And camera lens（5）, the ware box（2）And camera lens（5）Pass through
Connector（4）It is detachably connected, the ware box（2）Bottom is made up of high light transmissive material, the ware box（2）The thickness of bottom is less than
0.7mm, the camera lens（5）It is connected with zooming adjustment system.
2. a kind of Tissue Culture Dish as claimed in claim 1, it is characterised in that：The ware box（2）Surface is evenly equipped with some trainings
Foster groove, the culture tank rectangular array arrangement, the culture tank is rounded, and the horizontal and vertical upper two adjacent culture tank is equal
By connecting canal connection, the depth of the connection canal is less than the depth of culture tank.
3. a kind of Tissue Culture Dish as claimed in claim 2, it is characterised in that：Horizontal positioned at the culture tank edge of array edges
Connection canal is connected with to longitudinal direction, the spacing >=0.02cm between two culture tank, the depth of the culture tank is 0.47cm, institute
State the depth≤0.21cm of connection canal.
4. a kind of Tissue Culture Dish as claimed in claim 1 or 2, it is characterised in that：The connector（4）With ware box（2）It
Between be connected with gain piece（3）, the gain piece（3）Including the photic zone one being linked in sequence from top to bottom, gain layer and photic zone two,
The thickness of the photic zone one and photic zone two is 0.08cm, and the thickness of the gain layer is 1.22mm, one He of the photic zone
Photic zone two is made by high light transmissive material.
5. a kind of Tissue Culture Dish as claimed in claim 3, it is characterised in that：The connector（4）Middle part is provided with stepped hole,
The stepped hole includes hole and lower opening, and the diameter in the upper hole is equal to gain piece（3）Diameter, the lower opening
Diameter is equal to camera lens（5）Diameter；The upper in the hole wall is provided with profile of tooth buckling groove, the gain piece（3）Bottom
It is provided with clamping engagement groove；The gain piece（3）With ware box（2）It is flexibly connected by screw thread；The camera lens（5）By screw thread with
Hole is flexibly connected.
6. a kind of Tissue Culture Dish as claimed in claim 5, it is characterised in that：The hole depth of the lower opening is equal to camera lens（5）'s
Thickness, the hole depth in the upper hole is equal to gain piece（3）Thickness.
7. a kind of Tissue Culture Dish as claimed in claim 6, it is characterised in that：The high light transmissive material is gathered for 37 parts by mass parts
Methyl methacrylate, 59 parts of polyamine
13 parts of methacrylic acids, 40 parts of SnO2With 18 parts of Sm2O3Composition, the polyamine n：M is 5：7, the SnO2And Sm2O3Grain
Footpath is less than 700nm.
8. a kind of Tissue Culture Dish as claimed in claims 6 or 7, it is characterised in that：The gain layer is gathered for 59 parts by mass parts
Amine
13 parts of methacrylic acids, 5 parts of InGaAs, 0.7 part of Graphene and 7 parts of YVO4Composition, the polyamine n：M is 5：7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610845234.5A CN106434342A (en) | 2016-09-24 | 2016-09-24 | Cell culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610845234.5A CN106434342A (en) | 2016-09-24 | 2016-09-24 | Cell culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106434342A true CN106434342A (en) | 2017-02-22 |
Family
ID=58167106
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610845234.5A Pending CN106434342A (en) | 2016-09-24 | 2016-09-24 | Cell culture dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106434342A (en) |
Citations (9)
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|---|---|---|---|---|
| CN1646613A (en) * | 2002-02-19 | 2005-07-27 | 光子－X有限公司 | Polymer nanocomposites for optical applications |
| CN1963663A (en) * | 2006-12-07 | 2007-05-16 | 上海交通大学 | Photography system for welding process of compound heat reservoir in carbon dioxide laser-melting argon-arc welding |
| WO2011135772A1 (en) * | 2010-04-28 | 2011-11-03 | 株式会社東海ヒット | Sliding contact cover used for incubator for observation by microscope and incubator for observation by microscope provided therewith |
| CN102703301A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Darkroom used between installing seat of sequencing chip and CCD (Charge Coupled Device) camera |
| CN102703310A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | CCD (charge coupled device) camera directly coupled with sequencing chip |
| CN202945247U (en) * | 2012-12-07 | 2013-05-22 | 广西壮族自治区妇幼保健院 | Human embryo culture vessel with single culture, co-culture and group culture advantages |
| CN203960183U (en) * | 2014-05-14 | 2014-11-26 | 杨栎 | A kind of darkroom connecting joint for CCD camera |
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-
2016
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|---|---|---|---|---|
| CN1646613A (en) * | 2002-02-19 | 2005-07-27 | 光子－X有限公司 | Polymer nanocomposites for optical applications |
| CN1963663A (en) * | 2006-12-07 | 2007-05-16 | 上海交通大学 | Photography system for welding process of compound heat reservoir in carbon dioxide laser-melting argon-arc welding |
| WO2011135772A1 (en) * | 2010-04-28 | 2011-11-03 | 株式会社東海ヒット | Sliding contact cover used for incubator for observation by microscope and incubator for observation by microscope provided therewith |
| CN102703301A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Darkroom used between installing seat of sequencing chip and CCD (Charge Coupled Device) camera |
| CN102703310A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | CCD (charge coupled device) camera directly coupled with sequencing chip |
| CN202945247U (en) * | 2012-12-07 | 2013-05-22 | 广西壮族自治区妇幼保健院 | Human embryo culture vessel with single culture, co-culture and group culture advantages |
| CN203960183U (en) * | 2014-05-14 | 2014-11-26 | 杨栎 | A kind of darkroom connecting joint for CCD camera |
| US20150344625A1 (en) * | 2014-06-02 | 2015-12-03 | Samsung Electronics Co., Ltd. | Polyimide film, method of preparing polyimide film, optical device including polyimide film |
| CN204125459U (en) * | 2014-10-03 | 2015-01-28 | 亳州师范高等专科学校 | A kind of biological culture laboratory apparatus being convenient to observe |
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