CN106389472A - Sturgeon oil with skin repairing functions, and preparation method thereof - Google Patents
Sturgeon oil with skin repairing functions, and preparation method thereof Download PDFInfo
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- CN106389472A CN106389472A CN201610729469.8A CN201610729469A CN106389472A CN 106389472 A CN106389472 A CN 106389472A CN 201610729469 A CN201610729469 A CN 201610729469A CN 106389472 A CN106389472 A CN 106389472A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/60—Fish, e.g. seahorses; Fish eggs
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/02—Refining fats or fatty oils by chemical reaction
- C11B3/04—Refining fats or fatty oils by chemical reaction with acids
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/02—Refining fats or fatty oils by chemical reaction
- C11B3/06—Refining fats or fatty oils by chemical reaction with bases
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/10—Refining fats or fatty oils by adsorption
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Abstract
The invention discloses a sturgeon oil with skin repairing functions, and a crude preparation method and a refining method thereof. The sturgeon oil is extracted from sturgeon ovaries or spermaries. The refining method comprises steps of degumming, deacidification, decoloration, and deodorization. The sturgeon oil prepared via the above method possesses no peculiar smell, and antioxidant functions and skin repairing functions.
Description
Technical field
The present invention relates to a kind of sturgeon oil and preparation method thereof, more particularly, to one kind have antioxidation and skin repair
Sturgeon oil of function and preparation method thereof.
Background technology
Various reactive oxygen free radical are that organism constantly produces during the vital movement of oxidative metabolism, various in body
Enzyme system effect is lower and is constantly reduced, and removes, maintains the normal function of cell.The old and feeble generation with disease and the close phase of oxidation
Close, produce the various active oxygen such as hydroxy radical, superoxide anion, inorganic peroxide, organic peroxide incessantly freely
Base.Lipid peroxidation, protein denaturation, DNA ruptures, and enzyme inactivates, and biofilm structure damages and causes body that pathological changes occur.Pass through
Supplement the generation that the Natural antioxidant can suppress body oxygen-derived free radicals, remove excessive oxygen-derived free radicals and keep fit, delay
The aging course of body.
In fish oil be rich in polyunsaturated fatty acid, there is antioxidation, can be used for the health cares such as cardiovascular and cerebrovascular vessel it can also be used to
Skin repair.But in actual applications because it has abnormal flavour and few significant function is pointed to, its application is restricted.
Therefore, those skilled in the art is devoted to developing a kind of free from extraneous odour, has antioxidation and skin repair function
Sturgeon oil and preparation method.
Content of the invention
In view of the drawbacks described above of prior art, the technical problem to be solved is to provide a kind of free from extraneous odour, tool
There are antioxidation and the sturgeon oil of skin repair function.
The sturgeon oil that the present invention provides is extracted and is obtained by the ovary of Acipenser Sinensis or spermary.
The present invention also provides a kind of rough Preparation Method of sturgeon oil it is characterised in that comprising the following steps：
A. Acipenser Sinensis ovary or spermary is taken to thaw, by solid-liquid ratio 1:1.5 addition water, are homogenized using homogenizer, obtain organizing even
Slurry；
B. the tissue homogenate in step a is heated up to 45 DEG C；
C. the tissue homogenate being heated up to 45 DEG C in rapid b adds 10% caustic lye of soda, until the pH of tissue homogenate is extremely
8, it is incubated 8min, in mass ratio 1.5% ratio adds sodium chloride in tissue homogenate, continues to be incubated 15 minutes at 45 DEG C；
D. the tissue homogenate in step c is centrifuged, takes supernatant, obtain detached thick sturgeon oil.
The present invention also provides a kind of sturgeon oil process for purification it is characterised in that comprising the following steps：
A. degumming；
B. deacidification；
C. decolour；
D. deodorize.
Further, step a adopts phosphoric acid degumming method, and thick sturgeon oil is heated to 80 DEG C, adds 60% in fish oil
Phosphoric acid, after stirring, centrifugation removes the precipitation of colloid, obtains supernatant, as degumming sturgeon oil, wherein 60% phosphoric acid with
The mass ratio of thick sturgeon oil is 1：100.
Further, step b adopts sodium hydroxide deacidification method, comprises the following steps：
(1) measure sturgeon oil acid value, calculate alkali charge, prepare 15%NaOH solution according to required alkali charge, by sturgeon
Fish fish oil is heated to 40 DEG C, the 15%NaOH solution needed for being slowly added to calculate in 20min-30min；
(2) continue stir sturgeon oil, be warming up to 60 DEG C, be centrifuged after stratification, precipitation；
(3) add 8% light salt brine to sturgeon oil, wash to neutrality, 4000r/min is centrifuged 15 minutes, collects supernatant
Liquid, obtains deacidification sturgeon oil, wherein 8% light salt brine and the ratio of sturgeon oil are 20:100.
Further, step c includes for sturgeon oil being heated to 60 DEG C, adds decolorising agent, sucking filtration after stirring 30min, obtains
To filtrate, wherein decolorising agent and the mass ratio of sturgeon oil are 10：100.
Further, decolorising agent is attapulgite Palygorskite.
Further, step d leads to nitrogen deodorization method using decompression, under the conditions of vacuum 0.095MPa, to taking off in step c
Color sturgeon oil is smoothly passed through nitrogen, is warming up to 85 DEG C, completes after keeping 50min.
Fish oil is a kind of oils and fatss extracting from greasiness Fish, is polyunsaturated fatty acid (DHA and EPA) rich in ω -3, tool
There is antiinflammatory, adjust the health benefits such as blood fat.But, because fish generally has fishy smell, make the fish oil extracting from fish have fishy smell,
And the fishlike smell degree of different cultivars is different and also different from the fish oil fishy smell degree of the different parts extraction of fish, therefore select
Select the kind of suitable fish, and extract part is conducive to reducing the fishy smell of fish.Find there is the three of migration characteristic through experiment
Civilian fish, rainbow trout, Acipenser Sinensis, refined fish, magnificent sub- fishlike smell are less, and Acipenser Sinensis is especially prominent, and therefore, the present invention selects Acipenser Sinensis as raw material system
Standby fish oil, can reduce the fishy smell of prepared fish oil effectively.
In sturgeon oil, ω -3 is that polyunsaturated fatty acid has free radical base Scavenging activity, inhibited apoptosis, promotes
Cell proliferation, protection biomembrane participates in the agglutination after tissue injury, and in present market application, fish oil is usually used in edible guarantor
Strong, and the present invention passes through zoopery it was demonstrated that the effect to skin repair for the sturgeon oil, can be used for external application, repairs skin shallow
Degree is scalded.
Sturgeon oil has non-oxidizability, and its own is oxidized easily and lost efficacy, and therefore, in preparation process, adds anti-
Oxidation component can effectively reduce the speed of its own oxidation, increases the shelf-life.
The cold water fish oil that the present invention selects has low fishy smell, high antioxidant and the function of repairing skin.
Technique effect below with reference to design, concrete structure and generation to the present invention for the accompanying drawing is described further, with
It is fully understood from the purpose of the present invention, feature and effect.
Brief description
Fig. 1 is middle sturgeon oil and the ascorbic acid DPPH free radical scavenging effect of a preferred embodiment of the present invention
Comparison diagram；
Fig. 2 is the om observation result of 1 day each group wound tissue after skin scald in one embodiment of the invention, wherein a
For simple scald group, b is silver-colored zinc frost group, and c is sturgeon oil group；
Fig. 3 is the om observation result of 3 days each group wound tissues after skin scald in one embodiment of the invention, wherein a
For simple scald group, b is silver-colored zinc frost group, and c is sturgeon oil group；
Fig. 4 is the om observation result of 18 days each group wound tissues after skin scald in one embodiment of the invention, wherein a
For simple scald group, b is silver-colored zinc frost group, and c is sturgeon oil group.
Specific embodiment
The research of embodiment 1 sturgeon oil antioxidant activity
1st, light alkali water-swollen squid Acipenser Sinensis ovary fish oil
After taking some defrostings of Acipenser Sinensis ovary, solid-liquid ratio 1:1.5 addition tap waters, tissue homogenate device be homogenized, heat 45 DEG C with
10% caustic lye of soda adjusts pH to 8, is incubated 8min, adds sodium chloride in 1.5% ratio, after continuing insulation 15 minutes, is centrifuged (5
000r/min, 15min) detached crude fish oil.
2nd, Acipenser Sinensis ovary pyloric fat acid
Degumming:Using phosphoric acid degumming method.Mainly remove dephosphorization vinegar and impurity, sturgeon oil is heated to 80 DEG C, by 1% mass
Ratio adds 60% phosphoric acid, and after being sufficiently stirred for, centrifugation removes colloid.
Deacidification:Using sodium hydroxide deacidification method, measure sturgeon oil acid value, calculate alkali charge, be configured to 15%NaOH molten
Liquid, fish oil is heated to 40 DEG C, is slowly added in 20min-30min.Stirring, it is warming up to 60 DEG C, stratification centrifugation
Remove soap stock precipitation.Light salt brine (8%) is added to wash to neutrality in fish oil 20% ratio, 4000r/min is centrifuged 15 minutes, collects
Supernatant is deacidification sturgeon oil.
Decolouring:Sturgeon oil is heated to 60 DEG C, is proportionally added into 10% decolorising agent (attapulgite Palygorskite),
It is stirred continuously 30min sucking filtration.
Deodorize:Using the logical nitrogen deodorization method of decompression, under the conditions of vacuum 0.095MPa, smoothly it is passed through nitrogen, is warming up to
85 DEG C of 50min complete.
3rd, testing index method
Fatty acid compositional analysis Ge-MS method.
4th, result
5th, sturgeon oil DPPH radical scavenging activity measures
Various reactive oxygen free radical are that organism constantly produces during the vital movement of oxidative metabolism, various in body
Enzyme system effect is lower and is constantly reduced, and removes, maintains the normal function of cell.The old and feeble generation with disease and the close phase of oxidation
Close, produce the various active oxygen such as hydroxy radical, superoxide anion, inorganic peroxide, organic peroxide incessantly freely
Base.Lipid peroxidation, protein denaturation, DNA ruptures, and enzyme inactivates, and biofilm structure damages and causes body that pathological changes occur.Wish
The generation of body oxygen-derived free radicals can be suppressed by supplementing the Natural antioxidant, remove excessive oxygen-derived free radicals and keep fit,
Delay the aging course of body.
Fish oil has antioxidant activity rich in unsaturated fatty acid and appears in the newspapers.Acipenser Sinensis ovary fish oil correlational study rarely has report
Road.Common Study on Its Antioxidant Activity in Vitro method has rhodanate method, thiobarbituricacidα- method, ORAC method etc.,
1,1- diphenyl -2- picryl phenylhydrazine method (DPPH) because easy, quick, sensitive the features such as be often first-selection.Therefore adopt DPPH free radical
Removing method simultaneously compares the radical scavenging activity testing sturgeon oil with ascorbic acid, is that sturgeon oil above carries in antioxidant activity
For basic data.
Instrument and reagent
Experimental technique：
Dehydrated alcohol prepares DPPH solution 0.04mg/ml；Prepare ascorbic acid 1mg/ml storing solution, be diluted to series concentration
(40,50,60,70 and 80 μ g/ml)；Sturgeon oil prepare series concentration (2,6,10,14,18mg/m).Respectively take 2ml ascorbic acid
With sturgeon oil add 2mlDPPH solution, mix, room temperature place 30min after, 5000r/min be centrifuged 10min, take supernatant in
Light absorption value is surveyed at 517nm.DPPH free radical scavenging activity is pressed following equation and is calculated:
A0：Dehydrated alcohol+DPPH solution light absorption value；
A1：Sample solution+DPPH solution light absorption value；
A2：Sample solution+dehydrated alcohol light absorption value.
Experimental result：
With sturgeon oil concentration, DPPH free radical scavenging activity is fitted, (Y is DPPH free radical scavenging as shown in Figure 1
Rate, X is fish oil concentration).Its Scavenging activity of elimination effect of the DPPH free radical of table 1 sturgeon oil is all with fish oil mass concentration
Increase and increase, mass concentration is bigger, and Scavenging activity is stronger, and 2mg/ml-10mg/ml rises substantially, preferable dosage-efficiency
Dependency, 10mg/ml has a gentle process, but the mass concentration more stronger trend of major clean-up operation ability is obvious.
Table 1 sturgeon oil and ascorbic acid DPPH free radical scavenging effect
| Sturgeon oil | mg/ml | 2.0 | 6.0 | 10.0 | 14.0 | 18.0 |
| Ascorbic acid | μg/ml | 40 | 50 | 60 | 70 | 80 |
| Sturgeon oil | Clearance rate % | 11.53 | 28.31 | 60.42 | 64.85 | 69.58 |
| Ascorbic acid | Clearance rate % | 17.53 | 31.86 | 40.23 | 60.55 | 78.2 |
It is in line that sturgeon oil has obvious scavenging action (LC50=10.03mg/ml) and fish oil concentration to DPPH free radical
Sexual intercourse, that is, fish oil concentration is bigger, and clearance rate is stronger.Illustrate that the superoxide radical of sturgeon oil has certain Scavenging activity.
The impact to wounds in scalded rats for embodiment 2 sturgeon oil
Sturgeon oil refines from Acipenser Sinensis ovary, rich in omega-3 polyunsaturated fatty acidses.Omega-3 polyunsaturated fatty acidses
There are free radical base Scavenging activity, inhibited apoptosis, promote cell proliferation, protection biomembrane participates in the healing after tissue injury
Process.The impact to light degree Ⅱ wound tissue healing for the sturgeon oil of this experiment preliminary observation is it is intended that sturgeon oil is applied to
Scalded tissue reparation provides theoretical foundation.
1. material and method
Wistar male rat, body weight about 250-300g Wuhan University animal center；
Acipenser Sinensis ovary fish oil, the excellent company in Shanghai；
Silver-colored zinc frost Huaxin Biochemical Medical Dressing Co., Ltd., Xinxiang City；
Animal model
Wistar rat 66 animal ad libs, water.Observe one week before experiment.It is randomly divided into simple scald group, silver-colored zinc
White treatment group and sturgeon oil treatment group, every group 22.Before animal is scalded, lose hair or feathers in 1d back, pentobarbital sodium abdominal cavity note during experiment
Penetrate anesthesia, hair removal section is placed in 15s in 70 DEG C of waters bath with thermostatic control, make 2cmx3cm (10%TBSA) light degree Ⅱ scalding model (through pathology
Section confirms).Each group wound surface respectively use normal saline gauze, silver-colored zinc frost gauze, sturgeon oil gauze cover, each wound surface respectively at
At once be administered after wound, ld-3d every 8h dressing once, hereafter every d dressing once, until wound healing.Wound healing is observed:With
The silly skin of wound surface comes off, and new epithelium is generated as healing standard.
2 Testing index
Wound surface situation is observed during daily dressing, red and swollen situation of change, if be dried, edema wound oozes out degree, have or not point
Secretion, whether inflammatory wound surface occurs；Observe wound healing situation, complete epithelium be whether there is using Microscopic observation and cover as wound surface
Healing criterion, records wound healing time.
Wound healing rate rat 3d, 6d, 9d, 12d, 15d and 18d record Wound healing rate after wound.Based on following equation
Calculate Wound healing rate:Wound healing rate=(original wound surface area-do not heal wound surface area)/original wound surface area × 100%.
Pathological study：Each group animal respectively at wound after 1,3,18d (n=6) anesthesia after from wound surface cut about 1cm ×
The wound tissue of 1cm, is fixed with 37% formaldehyde after drawing materials, specimens paraffin embedding slices, under hematoxylin-eosin staining (H-E) light microscopic
Tissues observed Pathologic changes.
Statistical analysis data is represented with x ± s, carries out variance analyses, P using SPSS11.0<0.05 have for difference aobvious
Work property.
Experimental result
After wound, 1d-3d scald group wound surface is red and swollen substantially, the notable swelling of corium, and skin wound has a small amount of liquid to ooze out, wound surface
Periphery substantially exceeds, and silver-colored zinc frost group is taken second place, and sturgeon oil group is the lightest；After 3d, shallow three group wound surface of II degree scald are all dry, successively
Form dry crusts.Early stage scalds visible papillary layer of corium under simple wound group mirror obvious congested, edema and leukocyte infiltration, collagen fiber
Discrete (Fig. 2, Fig. 3).Under early stage silver-colored zinc frost group and sturgeon oil group mirror, inflammation behaves like simple scald group, and after wound, 3d two controls
Treatment group and the obvious recovery of scald group.After rat shallow II degree scald from the 3rd day, obvious granulation tissue in treatment group's wound surface
Hypertrophy, 3d granulation a small amount of hamartoplasia phenomenon after simple scald group wounds.18d each group rat wound surface heals substantially, healing
Wound surface has Mus staple length to go out (Fig. 4).
Rat Wound Healing rate is shown in Table 2.Experimental result shows:Shallow II degree Wound treating group and simple scald group healing rate ratio
Relatively, in wound after the 3rd, 6,9,12,15,18d healing percentage difference statistically significant.Using sturgeon oil and silver-colored zinc frost treatment
Group has, compared with matched group group, the effect remarkably promoting wound healing, wound surface cutaneous necrosis, and local woanded surface inflammation is anti-
Or granulation tissue undue growth phenomenon should be increased..
Effect in burn wound's infringement for the oxygen free radical injury earns widespread respect.Ischemia-reperfusion mistake in wound surface
Cheng Zhong, by impaired endotheliocyte, platelet, and the neutrophilic granulocyte of activation produces substantial amounts of oxygen-derived free radicals.Tissue of burn
The ability of scavenging activated oxygen reduces, and makes local concentration of oxygen free radicals too high, causes tissue injury.The damage to tissue for the oxygen-derived free radicals
Wound is mainly lipid peroxidation and the albuminous degeneration causing in biomembrane.The sturgeon oil extracting from Acipenser Sinensis ovary, is rich in
Omega-3 polyunsaturated fatty acidses.Omega-3 polyunsaturated fatty acidses have free radical base Scavenging activity, inhibited apoptosis, promote thin
Born of the same parents breed, and protection biomembrane participates in the agglutination after tissue injury.
Table 2 each group Wound healing rate compares (x ± s, %, n=10)
*:Compare P with simple scald group<0.01
With respect to simple scald group wound surface group after this result of study shallow II degree wound surface scald wound application sturgeon oil of display
Knit edema substantially to mitigate, the inflammatory reaction degree such as congested redness reduces, PATHOMORPHOLOGICAL OBSERVATION OF PULLORUM finds that it can mitigate local organization
Hyperemia, edema and leukocyte infiltration etc. show that sturgeon oil can mitigate oxygen-derived free radicals to tissue injury, have certain antiinflammatory action, promote
Enter the repair function of skin histology, have the certain significance to promoting burn wound healing.
The preferred embodiment of the present invention described in detail above.It should be appreciated that the ordinary skill of this area need not be created
The property made work just can make many modifications and variations according to the design of the present invention.Therefore, all technical staff in the art
Pass through the available technology of logical analysis, reasoning, or a limited experiment under this invention's idea on the basis of existing technology
Scheme, all should be in the protection domain being defined in the patent claims.
Claims (10)
1. a kind of have the sturgeon oil repairing skin function it is characterised in that described sturgeon oil is by the ovary of Acipenser Sinensis or essence
Nest extracts and obtains.
2. a kind of rough Preparation Method of sturgeon oil is it is characterised in that comprise the following steps：
A. Acipenser Sinensis ovary or spermary is taken to thaw, by solid-liquid ratio 1:1.5 addition water, are homogenized using homogenizer, obtain tissue homogenate；
B. the tissue homogenate in step a is heated up to 45 DEG C；
C. the tissue homogenate being heated up to 45 DEG C in rapid b adds 10% caustic lye of soda, until the pH to 8 of tissue homogenate, protects
Warm 8min, in mass ratio 1.5% ratio adds sodium chloride in tissue homogenate, continues to be incubated 15 minutes at 45 DEG C；
D. the tissue homogenate in step c is centrifuged, takes supernatant, obtain detached thick sturgeon oil.
3. a kind of sturgeon oil process for purification, it is characterised in that being refined thick sturgeon oil, comprises the following steps：
A. degumming；
B. deacidification；
C. decolour；
D. deodorize.
4. a kind of sturgeon oil process for purification as claimed in claim 2 is it is characterised in that described step a adopts phosphoric acid degumming
Method, slightly will be heated to 80 DEG C by sturgeon oil, add 60% phosphoric acid in fish oil, and after stirring, centrifugation removes the heavy of colloid
Form sediment, take supernatant, as degumming sturgeon oil, wherein 60% phosphoric acid is 1 with the mass ratio of thick sturgeon oil：100.
5. sturgeon oil process for purification as claimed in claim 2 is it is characterised in that described step b adopts sodium hydroxide deacidification
Method, comprises the following steps：
(1) measure sturgeon oil acid value, calculate alkali charge, prepare 15%NaOH solution according to required alkali charge, by Acipenser Sinensis fish
Oil is heated to 40 DEG C, the 15%NaOH solution needed for being slowly added to calculate in 20min-30min；
(2) continue stir sturgeon oil, be warming up to 60 DEG C, be centrifuged after stratification, precipitation；
(3) add 8% light salt brine to sturgeon oil, wash to neutrality, 4000r/min is centrifuged 15 minutes, collects supernatant, obtains
To deacidification sturgeon oil, wherein 8% light salt brine and the ratio of sturgeon oil are 20:100.
6. sturgeon oil process for purification as claimed in claim 2 it is characterised in that described step c include sturgeon oil plus
Heat, to 60 DEG C, adds decolorising agent, sucking filtration after stirring 30min, obtains filtrate, as decolour sturgeon oil.
7. sturgeon oil process for purification as claimed in claim 6 is it is characterised in that described decolorising agent is attapulgite
Palygorskite.
8. sturgeon oil process for purification as claimed in claim 6, wherein decolorising agent are 8 with the mass ratio of sturgeon oil：100-
12：100.
9. sturgeon oil process for purification as claimed in claim 2 is it is characterised in that described step d is using the logical nitrogen deodorize of decompression
Method, under the conditions of vacuum 0.095MPa, is smoothly passed through nitrogen to the decolouring sturgeon oil in step c, is warming up to 85 DEG C, protects
Complete after holding 50min.
10. a kind of application in repairing skin for sturgeon oil is it is characterised in that use Acipenser Sinensis fish as claimed in claim 1
Oil, is directly applied on skin.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107047809A (en) * | 2017-03-31 | 2017-08-18 | 福州海汇生物科技实业有限公司 | A kind of fish oil preparation technology |
| CN107400559A (en) * | 2017-08-10 | 2017-11-28 | 福建海富特生物科技股份有限公司 | A kind of method in semi continuous feed fish oil refinery practice addition antioxidant |
| CN107586591A (en) * | 2017-09-22 | 2018-01-16 | 浙江海洋大学 | A kind of preparation method of fish oil |
| KR102508983B1 (en) * | 2022-07-12 | 2023-03-10 | 이용광 | Method for preparing Immune boosting composition containing sturgeon water-soluble oil |
| KR102509800B1 (en) * | 2022-07-12 | 2023-03-14 | 이용광 | Method for preparing skin whitening functional composition containing sturgeon water-soluble oil |
| KR102510795B1 (en) * | 2022-07-12 | 2023-03-16 | 이용광 | Method for preparing wrinkle improvement composition containing sturgeon water-soluble oil |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009533399A (en) * | 2006-04-10 | 2009-09-17 | ラボラトワール・アゲッタン | Healing composition |
-
2016
- 2016-08-25 CN CN201610729469.8A patent/CN106389472A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009533399A (en) * | 2006-04-10 | 2009-09-17 | ラボラトワール・アゲッタン | Healing composition |
Non-Patent Citations (4)
| Title |
|---|
| 周本翔: "《水产加工增值技术》", 31 October 2009, 河南科学技术出版社 * |
| 王晓龙: "鲐鱼鱼油提取精制及抗氧化活性研究", 《中国优秀硕士学位论文全文数据库》 * |
| 邬薇薇等: "ω-3鱼油脂肪乳对大面积烧伤患者早期救治的影响", 《现代实用医学》 * |
| 邵淑双等: "俄罗斯鲟鱼雌雄生殖腺营养成分分析", 《中国食品科学技术学会第十二届年会暨第八居中美食品业高层论坛论文摘要集》 * |
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| CN107047809A (en) * | 2017-03-31 | 2017-08-18 | 福州海汇生物科技实业有限公司 | A kind of fish oil preparation technology |
| CN107400559A (en) * | 2017-08-10 | 2017-11-28 | 福建海富特生物科技股份有限公司 | A kind of method in semi continuous feed fish oil refinery practice addition antioxidant |
| CN107586591A (en) * | 2017-09-22 | 2018-01-16 | 浙江海洋大学 | A kind of preparation method of fish oil |
| KR102508983B1 (en) * | 2022-07-12 | 2023-03-10 | 이용광 | Method for preparing Immune boosting composition containing sturgeon water-soluble oil |
| KR102509800B1 (en) * | 2022-07-12 | 2023-03-14 | 이용광 | Method for preparing skin whitening functional composition containing sturgeon water-soluble oil |
| KR102510795B1 (en) * | 2022-07-12 | 2023-03-16 | 이용광 | Method for preparing wrinkle improvement composition containing sturgeon water-soluble oil |
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