CN106323803A - Method for rapidly determining coarse fiber content in plant-caused agricultural product - Google Patents
Method for rapidly determining coarse fiber content in plant-caused agricultural product Download PDFInfo
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- CN106323803A CN106323803A CN201610679929.0A CN201610679929A CN106323803A CN 106323803 A CN106323803 A CN 106323803A CN 201610679929 A CN201610679929 A CN 201610679929A CN 106323803 A CN106323803 A CN 106323803A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N5/00—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid
- G01N5/04—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid by removing a component, e.g. by evaporation, and weighing the remainder
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Abstract
The invention relates to a method for rapidly determining the coarse fiber content in a plant-caused agricultural product. A sample is put in a fiber filtration bag made of a specially-made fiber material and with the hole diameter of 25.0 microns, is boiled with acid and alkali for 30 minutes and then is cleaned with water, and drying, weighing and result calculation are performed. Compared with a national standard method, the method is more suitable for rapid determination of large-scale samples and is high in efficiency, low in cost, high in precision degree and accuracy and good in practicability. The method can serve as the supplement of the national standard method and is used for rapid determination of coarse fibers in plant-caused agricultural products (fruits, vegetables and the like) of the departments such as academic institutions and detection mechanism.
Description
Technical field
The present invention relates to coarse-fibred method in the plant-derived agricultural product of a kind of quickly mensuration.
Background technology
Along with the fast development of China's economy, stepping up of people's living standard, the requirement to food is increasingly finer,
In the food taken in, coarse-fibred content is fewer and feweri, modern " ciril disease " such as constipation, obesity, arteriosclerosis, heart and brain
Angiopathy, diabetes etc., seriously threaten the healthy of modern, and in the food of people, complementary diets fiber becomes
For the task of top priority.Plant crude fiber is commonly called as dietary fiber, with protein, fat, carbohydrate, vitamin, mineral, hydration
It is referred to as the seven major nutrient of the mankind.Scientific research proves, health to be ensured, needs to take in right amount crude fibre.Suitable is thick
Fiber intake, can help enterogastric peristalsis, promotes digesting and assimilating of food；Crude fibre also has powerful water absorption, when human body is taken the photograph
During the overnutrition entered, it can take out of external superfluous nutrition, and beneficially the excretion of feces, prevents constipation.And owing to it has
Huge adsorption group, can by numerous harmful, the poisonous factor is taken out of external, is genuine " intestinal tract cleaning husband ".Science
Research shows, edible highly purified crude fibre powder is as nutrient supplement food, the body constitution that can not only keep fit, moreover it is possible to the most pre-
The multiple diseases such as anti-coronary heart disease, diabetes.And be to solve current China public's crude fibre to take in the effective way of wretched insufficiency,
The health level improving the our people masses there is highly important meaning.
At present, measuring plant-derived coarse-fibred method is to use GB/T 5009.10-2003, and national standard method operates
Relatively time-consuming arduously, batch samples is difficult to carry out.Therefore, exploitation is a kind of quickly, be suitable for batch samples measures plant-derived
In agricultural product, the method for crude fiber content is significant for detection of agricultural products system.The method be utilize high temperature resistant, sour
The fiber filter bag of alkali sample is carried out acid boil, soda boiling, clean drying is weighed, thus is carried out coarse-fibred mensuration, is greatly saved
Time, improve precision and accuracy rate.At present, this kind of method has been widely used in many fields, as the most fine at feedstuff
In the mensuration of dimension, but this method mensuration of crude fiber content in plant-derived agricultural product not yet sees that relevant research should or relevant
With.And this method is compared with National Standard Method, simple, batch samples can be met and measure, its result accurately and reliably, can be as state
Strong the supplementing of mark method one, this is to abundant and to improve detection of agricultural products system significant.
This patent utilizes the fiber filter bag of special substance, has invented a kind of quick mensuration plant-derived agricultural product crude fibre and has contained
The method of amount, has realistic meaning to detection of agricultural products system from now on.
Summary of the invention
It is an object of the invention to provide a kind of method of crude fiber content in plant-derived agricultural product of quick mensuration.
The one that the present invention provides, to crude fibre method for measuring in plant-derived agricultural product, comprises the steps:
(1) sample type: relate to agricultural samples type and include: Cereals class, greengrocery, fruits.
(2) sample pre-treatments: carry out agricultural samples air-drying process (simultaneously carrying out determination of water), fat content > 5%
Agricultural product need to carry out defat in advance with ether or acetone soak 10min.
(3) sample determination: weigh appropriate air-dried test sample, in the fiber filter bag of constant weight, away from bag rim 0.5cm
Place's sealing, puts in fiber instrument, and soda acid respectively boils 30min, and boiling water washs 3 times, each 5min, is then taken out by filter bag, puts into
Drying in 105 DEG C of air dry ovens, after taking-up, cooling is weighed and calculates.
(4) data analysis: result shows, it is consistent with National Standard Method that filter bag method measures coarse-fibred content results in agricultural product.
In said method, described in step (1), relate to the agricultural product single sample at least 1kg of sample type.
In said method, described in step (2), sample is air-dried, after after also including air-drying, sample carries out pulverizing, and powder
Sample requirement crosses 1mm aperture sieve, and sample preserves in exsiccator.
In said method, when sample sample weighting amount described in step (3) is less than 1g, fineness requirement is at below 0.5mm.
The method that the present invention provides is simple, practical, can meet the quick mensuration of batch samples, use manpower and material resources sparingly, and
Greatly improving precision and the accuracy of method, the present invention is coarse-fibred mensuration and agricultural product in plant-derived agricultural product
The development of detection system provides technical support, has important promotional value.
What the present invention was compared with prior art had has the active effect that
(1) present invention has feature reproducible, that accuracy is high, easy and simple to handle, is practicable excellent process, more
It it is the important supplement to existing national standard method GB/T 5009.10-2003.
(2) assay method of the present invention coarse-fibred mensuration be applicable to plant-derived agricultural product.With common detection methods
Comparing, the method has that easy and simple to handle quickly applicable batch samples is carried out, and has good preci-sion and accuracy.
(3) coarse-fibred assay method in the plant-derived agricultural product of the present invention, studies agricultural product for agricultural research staff
Quality Detection provides technical support, to abundant and to improve detection of agricultural products system significant.
(4) assay method of the present invention is for findding out coarse-fibred content status in the plant-derived agricultural product of China, for disappearing
Expense person buys agricultural product and provides reference value, thus improves people's health level, has important productive value and scientific guidance meaning
Justice.
Detailed description of the invention
In order to enable the method that the present invention is more clearly described, the experimental technique to the present invention does with specifically below
Bright, the experimental technique used in following embodiment if no special instructions, is conventional method, material used, reagent etc.,
If no special instructions, the most commercially obtain.
Embodiment 1:
(1) sample type
Choose type for examination agricultural product to include: Cereals class, greengrocery, fruits, it is desirable to the weight of single sample is at least
1kg。
(2) sample pre-treatments
The bigger fresh sample of water content completes 20～30min at 100～105 DEG C in advance, then dries to constant weight for 60～70 DEG C.
The Cereals crop that water content is less to be also dried, and measures its moisture.1.00mm aperture sieve (sample sample weighting amount is crossed after pulverizing
During less than 1g, fineness is preferably at below 0.5mm).If such as Semen sojae atricolor or fat content in sample > 5%, need to be in advance with ether or third
Ketone soaks 10min and carries out defat.
(3) sample determination
Weighing appropriate amount of sample in the fiber filter bag of constant weight, seal with sealing machine, and be fixed in fiber instrument, soda acid respectively boils
Boiling 30min, then boiling water washs 3 times, each 5min, and filter bag is taken out after terminating by experiment, puts in 105 DEG C of air dry ovens and dries
Dry, cool down after taking-up, weigh and calculate.Contrasting with National Standard Method, each sample is repeated 6 times simultaneously.
(4) statistical analysis of experimental result
4.1 table 1 filter bag methods compare with National Standard Method measurement result
Note: in table 1, result is all in terms of butt
As it can be seen from table 1 measure 8 kinds of plant-derived agricultural product by two kinds of methods, test sample type relate to Cereals class,
Greengrocery, fruits, measure by filter bag method and National Standard Method simultaneously, carries out data analysis with t inspection, in α=0.05 time, t <
tα=0.05=2.571, therefore the coarse-fibred content of two kinds of method mensuration does not has significant difference, illustrates that filter bag method may be used for planting
Coarse-fibred mensuration in the property agricultural product of thing source.
Embodiment 2:
(1) sample type
It is chosen for Semen phaseoli radiati, rice, and single sample amount at least 1kg for examination agricultural product (Cereals class).
(2) sample pre-treatments
Cereals crop is dried in advance, and measures its moisture.(sample sample weighting amount is less than 1g to cross 1.00mm sieve after pulverizing
Time, fineness is preferably at below 0.5mm), and sample preserves in exsiccator.
(3) sample determination
Weigh appropriate amount of sample in the fiber filter bag of constant weight, with sealing machine in sealing at edge 0.5cm, and be fixed on fibre
In dimension instrument, soda acid respectively boils 30min, washs 3 times with boiling water, each 5min, is then taken out by filter bag and dries in 105 DEG C of drying baker
Dry, cool down after taking-up, weigh.If crude fat content > 5% agricultural product need to soak 10min with acetone or ether and carry out in advance
Defat.
(4) interpretation
Carrying out fiber determination experiment for examination agricultural product Cereals class (Semen phaseoli radiati and Semen Phaseoli) filter bag method, each sample is repeated 3 times,
Measurement result such as table 2.
Table 2 filter bag method measures the crude fiber content in Semen phaseoli radiati, Semen Phaseoli agricultural product
Note: result is in terms of butt.
It is from table 2 it can be seen that filter bag method measures the crude fiber content in the plant-derived agricultural product of Cereals class, reproducible,
Stability is high, and result is accurate.
Embodiment 3:
(1) sample type
It is chosen for Fructus Capsici, Herba Apii graveolentis and Fructus Mali pumilae, and single sample amount at least 1kg for examination agricultural product (greengrocery).
(2) sample pre-treatments
First 100～105 DEG C of the fresh sample that water content is bigger, dries 20～30min and completes, and then 60～70 DEG C are dried to become
Crisp, easily to clay into power, the time is unsuitable long.(when sample sample weighting amount is less than 1g, fineness preferably exists to cross 1.00mm aperture sieve after pulverizing
Below 0.5mm).And sample preserves in exsiccator.
(3) sample determination
Weigh appropriate amount of sample in the fiber filter bag of constant weight, with sealing machine in sealing at edge 0.5cm, and be fixed on fibre
In dimension instrument, soda acid respectively boils 30min, washs 3 times with boiling water, each 5min, is then taken out by filter bag and dries in 105 DEG C of drying baker
Dry, cool down after taking-up, weigh.If crude fat content > 5% agricultural product need to soak 10min with acetone or ether and carry out in advance
Defat.
(4) statistical analysis of experimental result
Carrying out fiber determination experiment for examination agricultural product fruit and vegerable class (Fructus Capsici and Fructus Mali pumilae) filter bag method, each sample is repeated 3 times,
Measurement result such as table 3.
Table 3 filter bag method measures the crude fiber content in Fructus Capsici and Fructus Mali pumilae agricultural product
Note: result is in terms of butt.
From table 3 it is observed that measure fruit and vegerable class by filter bag method to plant coarse-fibred content in source property agricultural product, repeatability
Good, accuracy is high, and result is accurate.
Method application experiment
Choose different types of plant-derived agricultural product respectively, carry out coarse-fibred determination test according to above-mentioned filter bag method.
By sample being put in the fiber filter bag that aperture is 25.0 μm that tailor-made fibre material is made, after acid, alkali respectively boil 30min,
With distilled water wash 3 times, each 5min, dry in 105 DEG C of air dry ovens, take out cooling after weigh and result of calculation.This
Inventive method, compared with National Standard Method, is more suitable for the quickly mensuration of batch samples, efficiency height, can reduce labor intensity, cost
Low and preci-sion and accuracy is high, practical.This invention can as national standard method supplement, for research institutions, testing machine
Gou Deng department is to quick mensuration coarse-fibred in plant-derived agricultural product (fruit and vegerable, grain etc.).
Claims (5)
1. a coarse-fibred method in the plant-derived agricultural product of quick mensuration, comprises the following steps:
(1) plant-derived agricultural samples (sample type relates to Cereals class, greengrocery and fruits) is collected.
(2) agricultural samples carries out air-drying process, (the freshest sample carries out determination of water), and in the agricultural product such as Semen sojae atricolor, crude fat contains
Measure high needs and carry out defat in advance with acetone or ether.
(3) sample determination: weigh appropriate air-dried test sample, wraps up, in bag rim with the fiber filter bag of constant weight
Sealing at 0.5cm, put in fiber instrument, soda acid respectively boils 30min, and boiling water washs 3 times, each 5min, is then taken out by filter bag,
Putting in 105 DEG C of air dry ovens and dry, after taking-up, cooling is weighed and calculates.
Method the most according to claim 1, it is characterised in that: the plant-derived agricultural product list related to described in step (1)
Individual sample at least 1kg.
Method the most according to claim 1 and 2, it is characterised in that: described in step (2), sample is air-dried, also include wind
Sample requirement after sample carries out pulverizing, and powder after dry crosses 1mm aperture sieve.
4. according to the method described in claim 1-3, it is characterised in that: when sample sample weighting amount described in step (3) is less than 1g, carefully
Degree requires at below 0.5mm.
Method the most according to claim 1 and 2, it is characterised in that: the crude fat content of sample described in step (2) >
5%, such as Semen sojae atricolor etc., need to soak 10min with acetone or ether and carry out defat in advance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610679929.0A CN106323803A (en) | 2016-08-16 | 2016-08-16 | Method for rapidly determining coarse fiber content in plant-caused agricultural product |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610679929.0A CN106323803A (en) | 2016-08-16 | 2016-08-16 | Method for rapidly determining coarse fiber content in plant-caused agricultural product |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2178357Y (en) * | 1993-10-12 | 1994-09-28 | 江苏省微生物研究所 | Quick-acting determination meter for coarse fibre |
| CN1284164A (en) * | 1998-02-06 | 2001-02-14 | 德斯夸瑞德发展有限公司 | Grain quality monitor |
| CN202049082U (en) * | 2011-04-14 | 2011-11-23 | 济南海能仪器有限公司 | Crude fiber test instrument |
| CN102323137A (en) * | 2011-09-07 | 2012-01-18 | 中国农业科学院草原研究所 | Fiber digestion apparatus |
| CN102539274A (en) * | 2012-01-11 | 2012-07-04 | 山东农业大学 | Fiber screen bag and method for rapidly determining coarse fiber |
| CN104062411A (en) * | 2013-03-22 | 2014-09-24 | 华中农业大学 | Method for determining crude fiber content in citrus by texture determination |
| CN205027594U (en) * | 2015-09-22 | 2016-02-10 | 汝州同富科技服务有限公司 | A disappear and boil device for determining crude fiber content in fodder |
| CN105486800A (en) * | 2016-01-08 | 2016-04-13 | 袁秀芹 | Fiber analyzer |
| CN205210053U (en) * | 2015-12-15 | 2016-05-04 | 内蒙古民族大学 | Filter bag is used to fodder coarse fibre survey |
| CN105699301A (en) * | 2015-12-31 | 2016-06-22 | 深圳市芭田生态工程股份有限公司 | Method for determining coarse cellulose in agricultural product by means of spectral method |
-
2016
- 2016-08-16 CN CN201610679929.0A patent/CN106323803A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2178357Y (en) * | 1993-10-12 | 1994-09-28 | 江苏省微生物研究所 | Quick-acting determination meter for coarse fibre |
| CN1284164A (en) * | 1998-02-06 | 2001-02-14 | 德斯夸瑞德发展有限公司 | Grain quality monitor |
| CN1184467C (en) * | 1998-02-06 | 2005-01-12 | 泰克斯特兰系统公司 | Grain quality monitor |
| CN202049082U (en) * | 2011-04-14 | 2011-11-23 | 济南海能仪器有限公司 | Crude fiber test instrument |
| CN102323137A (en) * | 2011-09-07 | 2012-01-18 | 中国农业科学院草原研究所 | Fiber digestion apparatus |
| CN102539274A (en) * | 2012-01-11 | 2012-07-04 | 山东农业大学 | Fiber screen bag and method for rapidly determining coarse fiber |
| CN104062411A (en) * | 2013-03-22 | 2014-09-24 | 华中农业大学 | Method for determining crude fiber content in citrus by texture determination |
| CN205027594U (en) * | 2015-09-22 | 2016-02-10 | 汝州同富科技服务有限公司 | A disappear and boil device for determining crude fiber content in fodder |
| CN205210053U (en) * | 2015-12-15 | 2016-05-04 | 内蒙古民族大学 | Filter bag is used to fodder coarse fibre survey |
| CN105699301A (en) * | 2015-12-31 | 2016-06-22 | 深圳市芭田生态工程股份有限公司 | Method for determining coarse cellulose in agricultural product by means of spectral method |
| CN105486800A (en) * | 2016-01-08 | 2016-04-13 | 袁秀芹 | Fiber analyzer |
Non-Patent Citations (3)
| Title |
|---|
| 张森 等: "饲料粒度及分布对国标法和滤袋法测定粗纤维的影响及两种方法的差异比较", 《粮食与饲料工业》 * |
| 毛艳贞 等: "滤袋法测定棉籽粕中粗纤维含量的不确定度评定", 《中国饲料》 * |
| 王长文 等: "两种山野菜与蔬菜中粗纤维含量的比较研究", 《科技与企业》 * |
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Application publication date: 20170111 |