CN106318865B - A kind of nucleic acid extraction and the portable unit of gene magnification - Google Patents
A kind of nucleic acid extraction and the portable unit of gene magnification Download PDFInfo
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- CN106318865B CN106318865B CN201610961374.9A CN201610961374A CN106318865B CN 106318865 B CN106318865 B CN 106318865B CN 201610961374 A CN201610961374 A CN 201610961374A CN 106318865 B CN106318865 B CN 106318865B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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Abstract
A kind of nucleic acid extraction and the portable unit of gene magnification, including a nucleic acid extraction and pretreatment module and matched gene magnification module, nucleic acid extraction includes multi-functional heat with pretreatment module and covered, multi-functional heat lid is internal to be provided with four independent cavitys, each cavity lower end is connected with valve, valve connects with the upper end of joint, and the lower end of joint can connect with reagent chamber, and the bottom of joint is covered with one layer of nucleic acid extraction film；It is brought into close contact between each inside cavity of the piston on cavity top and multi-functional heat lid, the top of piston is connected with button；Gene magnification module includes being capable of two warm areas of straight reciprocating motion, support frame is fixed between two warm areas, multi-functional heat lid can combine closely with support frame, by driving warm area to do straight reciprocating motion, reagent chamber is set to be relatively moved between different warm areas, so as to provide different reaction temperatures for the sample in reagent chamber and reagent, gene magnification is realized, there is the advantages of portable, quick.
Description
Technical field
The present invention relates to polymerase chain reaction (PCR) technical field, and in particular to a kind of nucleic acid extraction and gene magnification
Portable unit.
Background technology
The nucleic acid of sample is extracted, by realizing that amplification is the purpose of round pcr in vitro.PCR amplification procedure is
Refer to and the archaeal dna polymerase in micro-biological sample is subjected to specific gene in the presence of catalyst according to the feature of DNA profiling
Amplification.Following three parts will be included in whole process：High-temperature denatured, process annealing and medium temperature primer extend.Normal PCR is based on
Three temperature changes realize this process, and a sample generally requires the cyclic process of 20-40 times, so as to realize the expansion of nucleic acid
Increase.
In terms of nucleic acid extraction, because extracting method is more complicated, generally requires various matched instrument and equipments and come
Into, such as centrifuge, liquid-transfering gun etc. corollary equipment, process is cumbersome, and equipment price is expensive.
In terms of the amplification in vitro of nucleic acid, traditional three temperature ranges, due to the temperature lag of instrument, temperature change
Time is longer；The microscope carrier of sample needs continuous switching temperature, it is necessary to be equipped with Palt original paper and radiating original paper etc., temperature becomes
The specific heat capacity of change body is larger, and system is limited by the principle of itself, and equipment instrument is heavier.
The content of the invention
The shortcomings that in order to overcome above-mentioned prior art, it is an object of the invention to provide a kind of nucleic acid extraction and gene magnification
Portable unit, simple and effective nucleic acid extraction and Rapid nucleic acid amplification procedure can be realized, have it is portable, quick the advantages of.
In order to achieve the above object, the technical scheme taken of the present invention is：
A kind of nucleic acid extraction and the portable unit of gene magnification, including a nucleic acid extraction are matched somebody with somebody with pretreatment module and therewith
The gene magnification module closed.
Described nucleic acid extraction includes multi-functional hot lid 3 with pretreatment module, and the multi-functional hot inside of lid 3 is provided with four independences
Lysate cavity 13, buffer solution cavity 14, the first PCR reaction reagents cavity 15, the 2nd PCR reaction reagents cavity 16, it is each
Be provided with a hollow out fastening ring 5 in the middle part of individual cavity, the upper and lower ends of hollow out fastening ring 5 respectively with the first spring 4 and second spring 6
It is connected, second spring 6 is in tension in normal work all the time, and the lower end of second spring 6 is connected with valve 7, and valve 7 is the
Closely chimeric with the outlet of the multi-functional hot internal cavity of lid 3 under the pulling force effect of two springs 6, the upper end of valve 7 and joint 8 connects
Connect, the lower end of joint 8 can connect with reagent chamber 10, and the bottom of joint 8 is covered with one layer of nucleic acid extraction film 9；First spring 4 exists
All the time impaction state is in during normal work, the upper end of the first spring 4 is connected with piston 2, and piston 2 is each with multi-functional hot lid 3
It is brought into close contact between individual inside cavity, the top of piston 2 is connected with button 1.
Described gene magnification module includes the first warm area 12-1 and the second warm area 12-3 on the other side, the first warm area
The fixed plate 12-4 that 12-1, the second warm area 12-3 are connected through, which is fixed on, is capable of in the mechanism of straight reciprocating motion, the first temperature
Area 12-1 and the second warm area 12-3 forms U trip temperatures module 12, and support frame 11 is fixed on the first warm area 12-1 and the second warm area 12-
Between 3, remain stationary as at work, multi-functional hot lid 3 can be combined closely by buckle structure with support frame 11, the first warm area
12-1, the second warm area 12-3 are provided with four relative U-shaped grooves, and a pair of fan-shaped spring leafs are fixed with each slot opening
12-2, the temperature independent control of two warm areas.
Described reagent chamber 10 is connected with multi-functional hot lid 3 by joint 8, and connected mode is using the chimeric knot of corrugated
Structure, to ensure the compactness of connection.
Described the first warm area 12-1, the second warm area 12-3 and spring leaf 12-2 using thermal conductivity it is good material processing and
Into.
The beneficial effects of the present invention are：
1st, the present invention is slow by the nucleic acid extraction in the nucleic acid preprocessing process of complexity, addition lysate, membrane filtration, addition
Instrument required for the processes such as fliud flushing and these processes is realized by multi-functional hot lid 3, simple in construction, cheap.
2nd, the present invention substitutes the temperature changing process of traditional three-temperature-zone by the cyclic amplification process of two warm areas, reduces temperature
Control power.
3rd, because dual temperature plot structure is simple, volume can accomplish within 80mm*80mm*80mm, reach portable purpose.
4th, due to not needing alternating temperature process, it is thus only necessary to mobile dual temperature area, accelerated the speed of gene magnification.
5th, by the matching of U trip temperatures module and fan-shaped spring leaf, reagent chamber is made to enter dual temperature area easily.
Brief description of the drawings
Fig. 1 is the structural representation of nucleic acid extraction and pretreatment module of the present invention.
Fig. 2 is the structural representation of gene magnification module of the present invention.
Embodiment
The present invention is described in detail below in conjunction with the accompanying drawings.
A kind of nucleic acid extraction and the portable unit of gene magnification, including a nucleic acid extraction are matched somebody with somebody with pretreatment module and therewith
The gene magnification module closed.
Reference picture 1, described nucleic acid extraction include multi-functional hot lid 3 with pretreatment module, and the multi-functional hot inside of lid 3 is provided with
Four independent lysate cavitys 13, buffer solution cavity 14, the first PCR reaction reagents cavity 15, the 2nd PCR reaction reagent cavitys
16, be provided with a hollow out fastening ring 5 in the middle part of each cavity, the upper and lower ends of hollow out fastening ring 5 respectively with the first spring 4 and the
Two springs 6 are connected, and second spring 6 is in tension in normal work all the time, and the lower end of second spring 6 is connected with valve 7, valve
Door 7 is closely chimeric with the outlet of the multi-functional hot internal cavity of lid 3 under the pulling force effect of second spring 6, ensures inside cavity
Reagent will not flow out, and valve 7 connects with the upper end of joint 8, and the lower end of joint 8 can connect with reagent chamber 10, the bottom of joint 8
Covered with one layer of nucleic acid extraction film 9；First spring 4 is in impaction state, the upper end of the first spring 4 and work in normal work all the time
Plug 2 is connected, and is brought into close contact between each inside cavity of piston 2 and multi-functional hot lid 3, the top of piston 2 is connected with button
1, during work, piston 2 can move down under the promotion of button 1, while the envelope that can be formed to multi-functional hot lid 3 and piston 2
Air in closed chamber body produces pressure, when pressure caused by compressed air is more than pulling force effect of the bottom second spring 6 to valve 7
When, valve 7 will be moved down under pressure, and valve 7 is opened, reagent outflow.
Reference picture 2, described gene magnification module include the first warm area 12-1 and the second warm area 12-3 on the other side, the
The fixed plate 12-4 that one warm area 12-1, the second warm area 12-3 are connected through, which is fixed on, is capable of in the mechanism of straight reciprocating motion,
First warm area 12-1 and the second warm area 12-3 forms U trip temperatures module 12, and support frame 11 is fixed on the first warm area 12-1 and second
Between warm area 12-3, remaining stationary as at work, multi-functional hot lid 3 can be combined closely by buckle structure with support frame 11,
First warm area 12-1, the second warm area 12-3 are provided with four relative U-shaped grooves, and a pair of fans are fixed with each slot opening
Shape spring leaf 12-2, the temperature independent control of two warm areas.
Described reagent chamber 10 is connected with multi-functional hot lid 3 by joint 8, and connected mode is using the chimeric knot of corrugated
Structure, to ensure the compactness of connection.
Described the first warm area 12-1, the second warm area 12-3 and spring leaf 12-2 using thermal conductivity it is good material processing and
Into to ensure the uniformity of cavity inside temperature, enabling the thermally equivalent of reagent chamber 10.
The present invention operation principle be：
1) the sample pretreatment stage：
1. primary sample is added in reagent chamber 10, and it is connected with joint 8；2. by the cracking of joint 8 and multi-functional hot lid 3
Interface corresponding to sap cavity body 13 is connected, and button 1 corresponding to pressing, injects lysate into reagent chamber 10；3. again by joint 8 with
Interface corresponding to the buffer solution cavity 14 of multi-functional hot lid 3 is connected, button 1 corresponding to pressing, and buffering is injected into reagent chamber 10
Liquid；It is 4. again that joint 8 is corresponding with the first PCR reaction reagents cavity 15 of multi-functional hot lid 3 or the 2nd PCR reaction reagents cavity 16
Interface be connected, button 1 corresponding to pressing, corresponding PCR reaction reagents are injected into reagent chamber 10；5. it is last, remove joint
8, reagent chamber 10 and multi-functional hot lid 3 are joined directly together, now, multi-functional hot lid 3 only has closed effect；
2) the gene magnification stage：
After the completion of the pretreatment work of sample, the joint 8 covered with nucleic acid extraction film 9 is used as consumptive material, in specimen preprocessing
It is dropped, multi-functional hot lid 3 is joined directly together with reagent chamber 10, and closely tied by buckle structure and support frame 11 after the completion of reason
Closing, support frame 11 is fixed between the first warm area 12-1 and the second warm area 12-3, remains stationary as at work, during real work,
When the first warm area 12-1, the second warm area 12-3 straight reciprocating motions, the top of reagent chamber 10 can be forced to hold spring leaf 12-2, into U-shaped
In groove, after spring leaf 12-2 resets, the cavity of a near cylindrical can be surrounded jointly with the U-shaped groove on warm area, so as to
Make reagent chamber 10 by intactly wrap up with wherein.
By driving the first warm area 12-1 and the second warm area 12-3 to do straight reciprocating motion, make reagent chamber 10 in different temperature
Moved between area, so as to provide different reaction temperatures for the sample in reagent chamber 10 and reagent, and then realize gene magnification.
Claims (3)
1. a kind of nucleic acid extraction and the portable unit of gene magnification, it is characterised in that：Including a nucleic acid extraction and pretreatment mould
Block and matched gene magnification module；
Described nucleic acid extraction includes multi-functional heat with pretreatment module and covers (3), and four independences are provided with inside multi-functional heat lid (3)
Lysate cavity (13), buffer solution cavity (14), the first PCR reaction reagents cavity (15), the 2nd PCR reaction reagent cavitys
(16), be provided with a hollow out fastening ring (5) in the middle part of each cavity, the upper and lower ends of hollow out fastening ring (5) respectively with the first bullet
Spring (4) is connected with second spring (6), and second spring (6) is in tension in normal work all the time, under second spring (6)
End is connected with valve (7), outlet of the valve (7) under the pulling force effect of second spring (6) with multi-functional heat lid (3) internal cavity
Close chimeric, valve (7) connects with the upper end of joint (8), and the lower end of joint (8) can connect with reagent chamber (10), joint (8)
Bottom covered with one layer of nucleic acid extraction film (9)；First spring (4) is in impaction state, the first bullet in normal work all the time
Spring (4) upper end is connected with piston (2), is brought into close contact between each inside cavity of piston (2) and multi-functional heat lid (3), living
The top of plug (2) is connected with button (1)；
Described gene magnification module includes the first warm area (12-1) and the second warm area on the other side (12-3), the first warm area
The fixed plate (12-4) that (12-1), the second warm area (12-3) are connected through, which is fixed on, is capable of in the mechanism of straight reciprocating motion,
First warm area (12-1) and the second warm area (12-3) form U trip temperatures module (12), and support frame (11) is fixed on the first warm area
Between (12-1) and the second warm area (12-3), remain stationary as at work, multi-functional heat lid (3) can be with branch by buckle structure
Support (11) is combined closely, and the first warm area (12-1), the second warm area (12-3) are provided with four relative U-shaped grooves, each recessed
A pair of fan-shaped spring leafs (12-2), the temperature independent control of two warm areas are all fixed with channel opening.
2. a kind of nucleic acid extraction according to claim 1 and the portable unit of gene magnification, it is characterised in that：Described examination
Agent chamber (10) is connected with multi-functional heat lid (3) by joint (8), and connected mode uses corrugated embedded structure, to ensure to connect
The compactness connect.
3. a kind of nucleic acid extraction according to claim 1 and the portable unit of gene magnification, it is characterised in that：Described
One warm area (12-1), the second warm area (12-3) and spring leaf (12-2) are process using the good material of thermal conductivity.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610961374.9A CN106318865B (en) | 2016-10-25 | 2016-10-25 | A kind of nucleic acid extraction and the portable unit of gene magnification |
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| CN201610961374.9A CN106318865B (en) | 2016-10-25 | 2016-10-25 | A kind of nucleic acid extraction and the portable unit of gene magnification |
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| CN106318865A CN106318865A (en) | 2017-01-11 |
| CN106318865B true CN106318865B (en) | 2018-03-16 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106635746B (en) * | 2017-02-14 | 2019-11-19 | 江西农业大学 | The helicase dependent amplification reaction unit of citrus disease biocontrol bacterial strain is quickly identified at room temperature |
| CN107384777A (en) * | 2017-09-04 | 2017-11-24 | 上海科源电子科技有限公司 | A kind of multi-temperature zone gene amplification device |
| CN112899151A (en) * | 2021-02-01 | 2021-06-04 | 青岛迪诺瓦基因科技有限公司 | Flowing liquid temperature changing device and using method thereof |
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