CN106290922B - ABO is positive and negative and RhD antigens bloodtypes identify combiner and authentication method - Google Patents
ABO is positive and negative and RhD antigens bloodtypes identify combiner and authentication method Download PDFInfo
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- CN106290922B CN106290922B CN201610834200.6A CN201610834200A CN106290922B CN 106290922 B CN106290922 B CN 106290922B CN 201610834200 A CN201610834200 A CN 201610834200A CN 106290922 B CN106290922 B CN 106290922B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
Abstract
The present invention relates to the blood testing technology for detection equipment in medical treatment, specifically ABO is positive and negative and RhD antigens bloodtypes identification combiner, the identification combiner include four ABO test tubes and a RhD antigen test tube, it is characterized in that：Four ABO test tubes connect it is in a row be integrally formed structure, each ABO test tubes are formed by upper and lower two sections, and epimere is straight tube, and hypomere is conical pipe；The RhD antigens test tube is to be connected with an outermost ABO test tube using activity structure, RhD antigen test tubes have straight tube body, a liquid suction pipe is connected in body lower tine, it is furnished with a sampling viewer in the bottom of RhD antigen test tubes, the method for identifying blood group is to use classical macroprocedure using above-mentioned identification combiner while carry out abo blood group and RhD bracket for blood grouping；The present invention solves existing ABO and RhD detection and separately carried out, and inconvenient for operation, the problems such as easily making mistakes, being inconvenient to achieve, is mainly used in health care industry.
Description
Technical field
The present invention relates to the detection technique in health care, specifically ABO is positive and negative and RhD antigens bloodtypes identification combiner and
Authentication method.
Background technology
Up to the present situation of all-level hospitals Blood Transfusion Dept. does bracket for blood grouping, if ABO blood group system is with the anti-A of standard, anti-B blood
Clear reagent and four kinds of blood group standard reagents of blood cell of Staphylococal Protein A and B antigens respectively plus patient serum and blood cell in test tube（Or glass
Piece cardboard or gel liquid pipe）Inside reacted, then four kinds of reaction results can draw the blood group result of ABO systems more than.
With developing at present if necessary to treatment of blood transfusion for epoch, match in addition to ABO systems must make blood typing,
The bracket for blood grouping of RhD antigens must more importantly be made, because it causes the adverse reaction of this blood group incompatibility to be even more serious or endanger
Danger.Therefore clinically very be strict with make RhD antigens blood group and meanwhile using treatment of blood transfusion and other gestation or other be immunized
The treatment and blocking of disease（As the pregnancy of RhD negative pregnant womens has, RhD positive babies are now female or son necessarily causes great danger）, i.e.,
The detection of RhD antigens be included in clinical prevention and treatment extremely important blood group system and included of equal importance with abo blood group
Must examine blood group system.
Abo blood group identification at present and RhD Antigen Identifications are two respective completely self-contained a set of detection programs, cumbersome
Complexity, easily make mistakes（Generally there are more people while identified, determine to easily cause mistake during corresponding relation）, on the other hand pass
System abo blood group identification be based on macroprocedure, be determined by manually observing result, it is determined that after the completion of test tube is lost,
Stationary state can not be formed, it is impossible to scan mug, consulted the files to later further consultation and bring difficulty.
There is hospital to start to use micro fast ABO bood type authentication method in recent years, dosage is the 1/5 of macroprocedure, during reaction
Between only with several seconds（And macroprocedure is 15 minutes or so）, reaction temperature is at 20-25 DEG C, and the change of these bracket for blood grouping factors is not
Beneficial to the detection of incomplete antibody, will also result in some ABO hypotypes blood groups can be difficult to differentiate or the disadvantage of missing inspection.It is micro quick
Bracket for blood grouping method is equally existed the problem of being inconvenient to scan mug.
The content of the invention
Present invention aim to solve traditional macroprocedure abo blood group identification to be separated and independently performed with RhD Antigen Identifications,
Complex operation, easily error, it has not been convenient to scan mug, and micro quick bracket for blood grouping method has flase drop, missing inspection, not smart
Accurate, same the problems such as being inconvenient to achieve, there is provided a kind of ABO is positive and negative and RhD antigens bloodtypes identify combiner and authentication method.
The present invention concrete scheme be：ABO is positive and negative and RhD antigens bloodtypes identification combiner, include four ABO test tubes and
One RhD antigen test tube, it is characterized in that：Four ABO test tubes connect it is in a row be integrally formed structure, each ABO test tubes are by upper and lower two
Section is formed, and epimere is straight tube, and hypomere is conical pipe；The RhD antigens test tube is to use slip-knot with an outermost ABO test tube
Structure connects, and RhD antigen test tubes have straight tube body, a liquid suction pipe is connected in body lower tine, match somebody with somebody in the bottom of RhD antigen test tubes
There is a sampling viewer, the sampling viewer has a transparent plastic capsule, and the top of transparent plastic capsule is provided with intubation, and this is inserted
Pipe is that the bottom that RhD antigen test tubes are inserted after RhD antigens test tube completes mixing liquor makes transparent plastic capsule and RhD antigen test tubes
Connection, is configured with a manual gasbag on transparent plastic capsule.
Heretofore described four ABO test tubes are that straight forming fixation is connected or linked into an integrated entity using connector.
Heretofore described RhD antigens test tube be using card or buckle structure be connected with outermost ABO test tubes it is in a row.
The epimere volume of heretofore described ABO test tubes is that the constant volume of ABO tests (meets standard practice instructions appearance
Amount).
The epimere of heretofore described ABO test tubes is highly 40 ± 10mm, and interior bore is 7-9mm；Hypomere highly for 10 ±
5mm, upper internal diameter are 5 ± 2mm.
In heretofore described RhD antigens test tube, from body bottom surface 4-10mm at the fork-join of liquid suction pipe, liquid suction pipe bending to
On be arranged vertically, face height thereon>4/5 body height.
Apply above-mentioned any one the bracket for blood grouping combiner progress ABO of power positive and negative in the present invention and the mirror of RhD antigens bloodtypes
Determine method, including step in detail below：
, prepare that one group of ABO is positive and negative and RhD antigens bloodtypes identification combiner, and five test tubes in combiner will be identified
Number consecutively is No. 1 test tube, No. 2 test tubes, No. 3 test tubes, No. 4 test tubes, No. 5 test tubes since RhD antigen test tubes side；Before operation
Independently placed with the sampling viewer of No. 1 test tube configuration；
, in 2-5 test tubes add neutral gel and fill test tube hypomere conical pipe part；
, appropriate RhD antibody is added in No. 1 test tube（That is anti-D）, appropriate anti-A is added in No. 2 test tubes（I.e.
The anti-A serum of standard）, appropriate anti-B is added in No. 3 test tubes（That is standard anti B serum）, appropriate Staphylococal Protein A is added in No. 4 test tubes
（That is A types standard blood cell）, appropriate B antigens are added in No. 5 test tubes（That is Type B standard blood cell）；
, be separately added into 1-3 test tubes the 5% blood cell suspension 0.1ml of examinee, in 4, No. 5 test tubes respectively plus
Enter the ml of serum 0.25 of examinee；
, by No. 1 test tube from combiner separate, will 2-5 test tubes shake mix after be put into incubator keep 37 DEG C of constant temperature
React 10-18 minutes；
, take out from incubator 2-5 test tubes and be put into centrifuge with 3400 revs/min of centrifugations 3 minutes, take out observation
As a result the abo blood group of examinee is determined as follows：The hypomere of test tube is observed, blood cell is whether there is and penetrates into bottom, be set with infiltration
Then the mixed liquor not aggegation in test tube epimere is expressed as "-", and no mixed liquor aggegation penetrated into then test tube epimere is expressed as "+"；
Then if No. 2, No. 5 test tubes be "+", No. 3, No. 4 test tubes are when being "-", examinee's blood group can be defined as A type blood；If No. 2,5
Number test tube is "-", No. 3, No. 4 test tubes are when being "+", and examinee's blood group can be defined as Type B blood；If No. 2, No. 3 test tubes be
"-", No. 4, No. 5 test tubes are when being "+", examinee's blood group can be defined as O-shaped blood；If No. 2, No. 3 test tubes be "+", No. 4, No. 5
When test tube is "-", examinee's blood group can be defined as AB type blood；
, add cohesion amine 2 in No. 1 test tube and drip and the ml of low ion buffer 0.65, then No. 1 test tube is put into centrifugation
Machine is taken out after 2 minutes with 3400 revs/min of centrifugations, extracts the useless of top out from the liquid suction pipe of No. 1 test tube using aspirator
Liquid, the clot for retaining bottom mix slag, estimate this clot and mix whether slag has aggegation, such as this experiment must reform without aggegation；Observation has
After aggegation, then mix addition liquefacient 2 in slag to clot and drip, and gently shake whether test tube observing mixing blood clot scatters, observe
Invisible spectro mixture, represent that RhD antibody for feminine gender, represents examinee's blood cell if aggegation is not scattered if aggegation is scattered
In have RhD antigens, as RhD positives blood group；
, the intubation that will sample viewer insert the connection of No. 1 test tube bottom, the manual gasbag on operation sampling viewer,
The transparent plastic capsule for making the mixed liquor in No. 1 test tube enter in sampling viewer, swept with No. 2, No. 3, No. 4, No. 5 test tubes and same send
Retouch instrument scanning mug.
The present invention program can also be by the advance filling hypomere in each ABO test tubes of neutral gel（Fill hypomere）, shape
Into the bracket for blood grouping combiner for adding neutral gel.It is substantially same that bracket for blood grouping method is carried out using this bracket for blood grouping combiner
Above method step, simply removes above-mentioned steps from.
The present invention makes abo blood group identification and RhD Antigen Identifications simultaneously by designing a set of special bracket for blood grouping combiner
Operated, it is simple and convenient, it is not error-prone.Particularly in abo blood group identification, it is layered by using neutral gel, makes blood
Condense or state of aggregation be not relatively fixed in test tube conical section, and sampling viewer is added in RhD antigen test tubes,
So as to convenient observation, scanning mug.And the present invention is that tracing to the source for blood group cross reaction has been stricted adhere to from method
Property operational procedure and the classical standard reagent dosage of routine test and the test tube method of model（When 37 DEG C of reaction temperatures and reaction
Between, condition）Come what is identified, therefore the result is that Suo Yuan and true, science, reliably.Invention also overcomes leakage simultaneously
The disadvantage of inspection.
Brief description of the drawings
Fig. 1 is the structural representation of present invention identification combiner；
Fig. 2 is the zoomed-in view for sampling viewer.
In figure：1-ABO test tubes, 2- neutral gels, 3-RhD antigen test tubes, 4- liquid suction pipes, 5- sampling viewers, 6- are transparent
Plastic bag, 7- manual gasbags, 8- intubations.
Embodiment
Example 1：It is first according to Fig. 1,2 one group of ABO of making are positive and negative and RhD antigens bloodtypes identify combiner.The identification combiner
Body includes four ABO test tubes 1 and a RhD antigens test tube 3, it is characterized in that：Four ABO test tubes 1 connect in a row be integrally formed
Structure, each ABO test tubes 1 are formed by upper and lower two sections, and epimere is straight tube, and hypomere is conical pipe；The RhD antigens test tube 3 be with
An outermost ABO test tube 1 is connected using activity structure, and RhD antigens test tube 3 has straight tube body, is connected in body lower tine
One liquid suction pipe 4, is furnished with a sampling viewer 5 in the bottom of RhD antigens test tube 3, and the sampling viewer 5 has a transparent modeling
Expect capsule 6, the top of transparent plastic capsule 6 is provided with intubation 8, and the intubation 8 is to insert RhD after RhD antigens test tube 3 completes mixing liquor
The bottom of antigen test tube 3 makes transparent plastic capsule 6 be connected with RhD antigens test tube 3, and one is configured with transparent plastic capsule 6 manually
Air bag 7.
Four ABO test tubes 1 described in the present embodiment are that straight forming fixation is connected or connects into one using connector
Body.
The test tubes of RhD antigens described in the present embodiment 3 are to connect into one with outermost ABO test tubes 1 using card or buckle structure
Row.
ABO test tubes described in the present embodiment 1 epimere volume is the constant volume of ABO tests.
ABO test tubes described in the present embodiment 1 epimere is highly 40 ± 10mm, specially 40mm, and interior bore is 7-9mm,
Specially 8mm；Hypomere is highly 10 ± 5mm, specially 10mm, and upper internal diameter is 5 ± 2mm, specially 5mm.
In the test tubes of RhD antigens described in the present embodiment 3, from body bottom surface 4-10mm at the fork-join of liquid suction pipe 4, it is specially
8mm, the bending of liquid suction pipe 4 are arranged vertically upwards, and face height is highly concordant with body thereon（Typically>4/5 test tube height）.
The method that bracket for blood grouping is carried out using the bracket for blood grouping combiner of above-mentioned making comprises the following steps：
, prepare that one group of above-mentioned ABO is positive and negative and RhD antigens bloodtypes identification combiner, and five in combiner will be identified
Test tube number consecutively since the side of RhD antigens test tube 3 is No. 1 test tube, No. 2 test tubes, No. 3 test tubes, No. 4 test tubes, No. 5 test tubes；
Sampling viewer 5 before operation with No. 1 test tube configuration is independently placed；
, in 2-5 test tubes add neutral gel 2 and fill test tube hypomere conical pipe part；, in No. 1 test tube
It is middle to add appropriate RhD antibody, appropriate anti-A is added in No. 2 test tubes, appropriate anti-B is added in No. 3 test tubes, in No. 4 test tubes
Appropriate Staphylococal Protein A is added, appropriate B antigens are added in No. 5 test tubes；
, be separately added into 1-3 test tubes the 5% blood cell suspension 0.1ml of examinee, in 4, No. 5 test tubes respectively plus
Enter the ml of serum 0.25 of examinee；
, by No. 1 test tube from combiner separate, will 2-5 test tubes shake mix after be put into incubator keep 37 DEG C of constant temperature
React 10-18 minutes；
, take out from incubator 2-5 test tubes and be put into centrifuge with 3400 revs/min of centrifugations 3 minutes, take out observation
As a result the abo blood group of examinee is determined as follows：The hypomere of test tube is observed, blood cell is whether there is and penetrates into bottom, be set with infiltration
Then the mixed liquor not aggegation in test tube epimere is expressed as "-", and no mixed liquor aggegation penetrated into then test tube epimere is expressed as "+"；
Then if No. 2, No. 5 test tubes be "+", No. 3, No. 4 test tubes are when being "-", examinee's blood group can be defined as A type blood；If No. 2,5
Number test tube is "-", No. 3, No. 4 test tubes are when being "+", and examinee's blood group can be defined as Type B blood；If No. 2, No. 3 test tubes be
"-", No. 4, No. 5 test tubes are when being "+", examinee's blood group can be defined as O-shaped blood；If No. 2, No. 3 test tubes be "+", No. 4, No. 5
When test tube is "-", examinee's blood group can be defined as AB type blood；
, add cohesion amine 2 in No. 1 test tube and drip and the ml of low ion buffer 0.65, then by No. 1 test tube be put into from
Scheming is taken out after 2 minutes with 3400 revs/min of centrifugations, and top is extracted out from the liquid suction pipe of No. 1 test tube using aspirator
Waste liquid, the clot for retaining bottom mix slag, estimate this clot and mix whether slag has aggegation, such as this experiment must reform without aggegation；Observation
After having aggegation, then mix addition liquefacient 2 in slag to clot and drip, and gently shake whether test tube observing mixing blood clot scatters, see
Invisible spectro mixture is examined, represents that RhD antibody for feminine gender, represents examinee's blood if aggegation is not scattered if aggegation is scattered
There are RhD antigens, as RhD positives blood group in ball；
, the intubation 8 for sampling viewer 5 inserted to the connection of No. 1 test tube bottom, the manual gas on operation sampling viewer 5
Capsule 7, the transparent plastic capsule 6 for making mixed liquor in No. 1 test tube enter in sampling viewer 5, with No. 2, No. 3, No. 4, No. 5 test tubes simultaneously
It is same to send scanner scanning mug.
Simultaneously more people's checked objects can also be carried out with survey inspection using the inventive method, i.e., it is accurate according to the number of checked object
Standby respective sets（Correspond）Bracket for blood grouping combiner, then each group repeat identified by above-mentioned steps.
Example 2：One group of bracket for blood grouping combiner is made by the same structure of example 1 first, then in each ABO test tube 1
Hypomere, which is filled, neutral gel 2, and diaphragm seal or closure are sealed with the top of ABO test tubes 1, forms one group and adds neutrality
The bracket for blood grouping combiner of gel 2.
The method for carrying out bracket for blood grouping using the above-mentioned bracket for blood grouping combiner for adding neutral gel 2 includes following step
Suddenly：
, prepare one group of above-mentioned bracket for blood grouping combiner for adding neutral gel 2, and by identify combiner in five
Individual test tube number consecutively since the side of RhD antigens test tube 3 is No. 1 test tube, No. 2 test tubes, No. 3 test tubes, No. 4 test tubes, No. 5 examinations
Pipe；Sampling viewer 5 before operation with No. 1 test tube configuration is independently placed；
, open 2-5 test tubes on diaphragm seal or lid, appropriate RhD antibody is added in No. 1 test tube, No. 2 examination
Appropriate anti-A is added in pipe, appropriate anti-B is added in No. 3 test tubes, appropriate Staphylococal Protein A is added in No. 4 test tubes, is added in No. 5 test tubes
Enter appropriate B antigens；
, be separately added into 1-3 test tubes the 5% blood cell suspension 0.1ml of examinee, in 4, No. 5 test tubes respectively plus
Enter the ml of serum 0.25 of examinee；
, by No. 1 test tube from combiner separate, will 2-5 test tubes shake mix after be put into incubator keep 37 DEG C of constant temperature
React 10-18 minutes；
, take out from incubator 2-5 test tubes and be put into centrifuge with 3400 revs/min of centrifugations 3 minutes, take out observation
As a result the abo blood group of examinee is determined as follows：The hypomere of test tube is observed, blood cell is whether there is and penetrates into bottom, be set with infiltration
Then the mixed liquor not aggegation in test tube epimere is expressed as "-", and no mixed liquor aggegation penetrated into then test tube epimere is expressed as "+"；
Then if No. 2, No. 5 test tubes be "+", No. 3, No. 4 test tubes are when being "-", examinee's blood group can be defined as A type blood；If No. 2,5
Number test tube is "-", No. 3, No. 4 test tubes are when being "+", and examinee's blood group can be defined as Type B blood；If No. 2, No. 3 test tubes be
"-", No. 4, No. 5 test tubes are when being "+", examinee's blood group can be defined as O-shaped blood；If No. 2, No. 3 test tubes be "+", No. 4, No. 5
When test tube is "-", examinee's blood group can be defined as AB type blood；
, add cohesion amine 2 in No. 1 test tube and drip and the ml of low ion buffer 0.65, then No. 1 test tube is put into centrifugation
Machine is taken out after 2 minutes with 3400 revs/min of centrifugations, extracts the useless of top out from the liquid suction pipe of No. 1 test tube using aspirator
Liquid, the clot for retaining bottom mix slag, estimate this clot and mix whether slag has aggegation, such as this experiment must reform without aggegation；Observation has
After aggegation, then mix addition liquefacient 2 in slag to clot and drip, and gently shake whether test tube observing mixing blood clot scatters, observe
Invisible spectro mixture, represent that RhD antibody for feminine gender, represents examinee's blood cell if aggegation is not scattered if aggegation is scattered
In have RhD antigens, as RhD positives blood group；
, the intubation 8 for sampling viewer 5 inserted to the connection of No. 1 test tube bottom, the manual gas on operation sampling viewer 5
Capsule 7, the transparent plastic capsule 6 for making mixed liquor in No. 1 test tube enter in sampling viewer 5, with No. 2, No. 3, No. 4, No. 5 test tubes simultaneously
It is same to send scanner scanning mug.
Claims (8)
1.ABO is positive and negative and RhD antigens bloodtypes identify combiner, includes four ABO test tubes and a RhD antigen test tube, and it is special
Sign is：Four ABO test tubes connect it is in a row be integrally formed structure, each ABO test tubes are formed by upper and lower two sections, and epimere is straight tube, under
Section is conical pipe；The RhD antigens test tube is to be connected with an outermost ABO test tube using activity structure, RhD antigens test tube tool
There is straight tube body, a liquid suction pipe is connected in body lower tine, be furnished with a sampling viewer in the bottom of RhD antigen test tubes, should
Sampling viewer has a transparent plastic capsule, and the top of transparent plastic capsule is provided with intubation, and the intubation is complete in RhD antigen test tubes
The bottom of RhD antigen test tubes is inserted after into mixing liquor makes transparent plastic capsule be connected with RhD antigen test tubes, on transparent plastic capsule
It is configured with a manual gasbag.
2. ABO according to claim 1 is positive and negative and RhD antigens bloodtypes identify combiner, it is characterized in that：Four ABO
Test tube is that straight forming fixation is connected or linked into an integrated entity using connector.
3. ABO according to claim 1 is positive and negative and RhD antigens bloodtypes identify combiner, it is characterized in that：The RhD antigens
Test tube be using card or buckle structure be connected with outermost ABO test tubes it is in a row.
4. ABO according to claim 1 is positive and negative and RhD antigens bloodtypes identify combiner, it is characterized in that：The ABO test tubes
Epimere be highly 40 ± 10mm, interior bore is 7-9mm；Hypomere is highly 10 ± 5mm, and upper internal diameter is 5 ± 2mm.
5. ABO according to claim 1 is positive and negative and RhD antigens bloodtypes identify combiner, it is characterized in that：The RhD antigens
In test tube, it is arranged vertically upwards from body bottom surface 4-10mm, liquid suction pipe bending at the fork-join of liquid suction pipe, thereon face height>4/5
Body height.
6. ABO according to claim 1 is positive and negative and RhD antigens bloodtypes identify combiner, it is characterized in that：In each ABO
Hypomere in test tube, which is filled, neutral gel, and diaphragm seal or closure are sealed with the top of ABO test tubes.
7. apply the identification combiner progress ABO of any one in claim 1-5 positive and negative and the identification side of RhD antigens bloodtypes
Method, it is characterized in that：Including step in detail below：
（1）, prepare that one group of ABO is positive and negative and RhD antigens bloodtypes identification combiner, and by five test tubes identified in combiner from
RhD antigen test tubes side starts number consecutively as No. 1 test tube, No. 2 test tubes, No. 3 test tubes, No. 4 test tubes, No. 5 test tubes；Operation before with
The sampling viewer of No. 1 test tube configuration is independently placed；
（2）, in 2-5 test tubes add neutral gel and fill test tube hypomere conical pipe part；
（3）, appropriate RhD antibody is added in No. 1 test tube, appropriate anti-A is added in No. 2 test tubes, is added in No. 3 test tubes suitable
Anti- B is measured, appropriate Staphylococal Protein A is added in No. 4 test tubes, appropriate B antigens are added in No. 5 test tubes；
（4）, be separately added into 1-3 test tubes the 5% blood cell suspension 0.1ml of examinee, be separately added into 4, No. 5 test tubes by
The ml of serum 0.25 of inspection person；
（5）, No. 1 test tube separated from combiner, 2-5 test tubes are shaken after mixing and be put into incubator and keep the reaction of 37 DEG C of constant temperature
10-18 minutes；
（6）, take out from incubator 2-5 test tubes and be put into centrifuge with 3400 revs/min of centrifugations 3 minutes, take out observation result
The abo blood group of examinee is determined as follows：The hypomere of test tube is observed, blood cell is whether there is and penetrates into bottom, infiltration is set with and then tries
Mixed liquor not aggegation in pipe epimere is expressed as "-", and no mixed liquor aggegation penetrated into then test tube epimere is expressed as "+"；Then such as
No. 2, No. 5 test tubes of fruit are "+", No. 3, No. 4 test tubes are when being "-", and examinee's blood group can be defined as A type blood；If No. 2, No. 5 examinations
Manage as "-", No. 3, No. 4 test tubes are when being "+", examinee's blood group can be defined as Type B blood；If No. 2, No. 3 test tubes be "-", 4
Number, No. 5 test tubes are when being "+", examinee's blood group can be defined as O-shaped blood；If No. 2, No. 3 test tubes be "+", No. 4, No. 5 test tubes
For "-" when, examinee's blood group can be defined as AB type blood；
（7）, add cohesion amine 2 in No. 1 test tube and drip and the ml of low ion buffer 0.65, then by No. 1 test tube be put into centrifuge with
3400 revs/min of centrifugations take out after 2 minutes, extract the waste liquid on top out from the liquid suction pipe of No. 1 test tube using aspirator, protect
Keep on file portion clot mix slag, estimate this clot mix slag whether have aggegation, such as without aggegation, this experiment must reform；Observation has aggegation
Afterwards, then mix addition liquefacient 2 in slag to clot to drip, and gently shake whether test tube observing mixing blood clot scatters, observe test tube
Interior mixture, represent that RhD antibody for feminine gender, represents have in examinee's blood cell if aggegation is not scattered if aggegation is scattered
RhD antigens, as RhD positives blood group；
（8）, the intubation that will sample viewer insert the connection of No. 1 test tube bottom, the manual gasbag on operation sampling viewer, make 1
The transparent plastic capsule that mixed liquor in number test tube enters in sampling viewer, with No. 2, No. 3, No. 4, No. 5 test tubes and with sending scanning
Instrument scans mug.
8. apply identification combiner progress ABO described in claim 6 positive and negative and the authentication method of RhD antigens bloodtypes, it is characterized in that：
Including step in detail below：
（1）, prepare to identify combiner described in one group of claim 7, and five test tubes identified in combiner are tried from RhD antigens
Pipe side starts number consecutively as No. 1 test tube, No. 2 test tubes, No. 3 test tubes, No. 4 test tubes, No. 5 test tubes；Match somebody with somebody before operation with No. 1 test tube
The sampling viewer put independently is placed；
（2）, open 2-5 test tubes on diaphragm seal or lid, appropriate RhD antibody is added in No. 1 test tube, in No. 2 test tubes
Appropriate anti-A is added, appropriate anti-B is added in No. 3 test tubes, appropriate Staphylococal Protein A is added in No. 4 test tubes, is added in No. 5 test tubes suitable
Measure B antigens；
（3）, be separately added into 1-3 test tubes the 5% blood cell suspension 0.1ml of examinee, be separately added into 4, No. 5 test tubes by
The ml of serum 0.25 of inspection person；
（4）, No. 1 test tube separated from combiner, 2-5 test tubes are shaken after mixing and be put into incubator and keep the reaction of 37 DEG C of constant temperature
10-18 minutes；
（5）, take out from incubator 2-5 test tubes and be put into centrifuge with 3400 revs/min of centrifugations 3 minutes, take out observation result
The abo blood group of examinee is determined as follows：The hypomere of test tube is observed, blood cell is whether there is and penetrates into bottom, infiltration is set with and then tries
Mixed liquor not aggegation in pipe epimere is expressed as "-", and no mixed liquor aggegation penetrated into then test tube epimere is expressed as "+"；Then such as
No. 2, No. 5 test tubes of fruit are "+", No. 3, No. 4 test tubes are when being "-", and examinee's blood group can be defined as A type blood；If No. 2, No. 5 examinations
Manage as "-", No. 3, No. 4 test tubes are when being "+", examinee's blood group can be defined as Type B blood；If No. 2, No. 3 test tubes be "-", 4
Number, No. 5 test tubes are when being "+", examinee's blood group can be defined as O-shaped blood；If No. 2, No. 3 test tubes be "+", No. 4, No. 5 test tubes
For "-" when, examinee's blood group can be defined as AB type blood；
（6）, add cohesion amine 2 in No. 1 test tube and drip and the ml of low ion buffer 0.65, then by No. 1 test tube be put into centrifuge with
3400 revs/min of centrifugations take out after 2 minutes, extract the waste liquid on top out from the liquid suction pipe of No. 1 test tube using aspirator, protect
Keep on file portion clot mix slag, estimate this clot mix slag whether have aggegation, such as without aggegation, this experiment must reform；Observation has aggegation
Afterwards, then mix addition liquefacient 2 in slag to clot to drip, and gently shake whether test tube observing mixing blood clot scatters, observe test tube
Interior mixture, represent that RhD antibody for feminine gender, represents have in examinee's blood cell if aggegation is not scattered if aggegation is scattered
RhD antigens, as RhD positives blood group；
（7）, the intubation that will sample viewer insert the connection of No. 1 test tube bottom, the manual gasbag on operation sampling viewer, make 1
The transparent plastic capsule that mixed liquor in number test tube enters in sampling viewer, with No. 2, No. 3, No. 4, No. 5 test tubes and with sending scanning
Instrument scans mug.
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| CN114236140A (en) * | 2021-12-27 | 2022-03-25 | 江苏贝索生物工程有限公司 | Blood type intelligent interpretation method based on test tube method |
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| JPH087215B2 (en) * | 1987-08-24 | 1996-01-29 | シュティフツング・フュア・ディアグノスティッシュ・フォルシュンク | Method for detecting antigen and / or antibody and test kit for detection |
| JP2009030997A (en) * | 2007-07-24 | 2009-02-12 | Olympus Corp | Blood group determining kit for type and screening and blood group determining device using the same |
| US8187538B2 (en) * | 2008-01-17 | 2012-05-29 | Ortho-Clinical Diagnostics, Inc. | Diluent wells produced in card format for immunodiagnostic testing |
| JP2010190732A (en) * | 2009-02-18 | 2010-09-02 | Beckman Coulter Inc | Microreactor container for blood test |
| CN201449350U (en) * | 2009-06-11 | 2010-05-05 | 贾璋林 | Blood-testing reagent plate |
| WO2012159275A1 (en) * | 2011-05-26 | 2012-11-29 | Siemens Aktiengesellschaft | Blood typing system |
| CN102435756A (en) * | 2011-09-21 | 2012-05-02 | 长春博迅生物技术有限责任公司 | Preparation method of detection card for detecting ABO and RhD blood groups |
| CN102680716A (en) * | 2012-06-07 | 2012-09-19 | 北京金豪制药股份有限公司 | ABO/RhD blood group antigen detection reagent card and preparation method thereof |
| CN203083997U (en) * | 2012-12-13 | 2013-07-24 | 江苏创生生物技术有限公司 | ABO and RhD blood group antigen detection card |
| CN205353104U (en) * | 2016-02-23 | 2016-06-29 | 英科新创(厦门)科技有限公司 | ABO&RhD blood group is stereotyped and is detected card |
| CN206002551U (en) * | 2016-09-20 | 2017-03-08 | 刘大基 | ABO is positive and negative and RhD antigens bloodtypes identify combiner |
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