CN106179075B - A kind of material point deposits formula mixing arrangement - Google Patents
A kind of material point deposits formula mixing arrangement Download PDFInfo
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- CN106179075B CN106179075B CN201510222297.0A CN201510222297A CN106179075B CN 106179075 B CN106179075 B CN 106179075B CN 201510222297 A CN201510222297 A CN 201510222297A CN 106179075 B CN106179075 B CN 106179075B
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Abstract
A kind of material point deposits formula mixing arrangement, including the first raw material cabin, the second raw material cabin, is equipped with the barrier layer for separating the second raw material in the first raw material and the second raw material cabin in the first raw material cabin in the second raw material cabin；The first hollow cavity and the second hollow cavity are respectively equipped in first raw material cabin and the second raw material cabin, thrust assemblies are provided in first hollow cavity, when thrust assemblies are mobile to the direction of the second raw material cabin, thrust assemblies front end punctures barrier layer, and press to the first raw material in the first hollow cavity in the second hollow cavity by the opening of barrier layer, the first raw material is mixed with the second raw material in the second hollow cavity.The present invention includes the mixing by realizing material between adjacent feed storehouse, first raw material cabin and the second raw material cabin of the invention can individually save material, and material is separated by barrier layer, thrust assemblies front end punctures barrier layer, and the first raw material in the first raw material cabin and the second raw material cabin sufficiently, is efficiently mixed with the second raw material.
Description
Technical field
The present invention relates to a kind of experiment consumptive materials more particularly to a kind of material point to deposit formula mixing arrangement.
Background technique
With the transformation of medical model and the continuous development of personalized medicine, there is an urgent need to quick, accurate for medical test circle
Detection means, Molecular Detection then plays unique advantage.The basis of Molecular Detection be analyze detected person histocyte,
Hair, anticoagulation or dry bloodstain and formaldehyde fixes, gene and its expression product in the tissue of paraffin embedding, by from point
Nucleic acid (DNA and RNA) detection is completed in sub- level, in disease once occurring symptom, sign and biochemical change even not yet occur
Before, detection can accurately just be made.
Currently, molecular detection technology mainly has making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip technology
Deng.Molecular Detection product is mainly used in the detection and physical examination of the clinical departments such as tumour, infection, heredity, Prenatal Screening
The heart, technical service center, third party testing agency and microbial rapid detection market etc..Currently, blood routine, cell
The measuring means such as, pathology and immunology develop towards automation, integration, standardization direction, but due to Molecular Detection
The full-automatic instrument platform of its own technical complexity, " from sample to result " is few or there are many insoluble skills
Art problem.The centrifugal column method used in field of biotechnology or paramagnetic particle method carry out nucleic acid extraction, generally require and are cracked, tied
Four steps such as conjunction, rinsing, elution, in addition subsequent making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip etc. are examined
Step is surveyed, keeps the full-automatic instrument of entire " from sample to result " very difficult, it is single with regard to effective component in each step
For mixing, the prior art mainly adopts the modes of hand mix, i.e., raw material or intermediate product are existed by artificial mode
Successively step and realizing successively is mixed in the reaction vessel of step, it is therefore apparent that the manual material used in the prior art
Hybrid mode, it is not only cumbersome, time-consuming and laborious.What is more also resides in whole operation process and be easy to cause pollution, influences to mention
The purity of object is taken, and material is difficult sufficiently, efficiently to be mixed, and influences experimental result.Therefore, in view of Molecular Detection its own
The full-automatic instrument platform of technical complexity, " from sample to result " is few or there are many insoluble technologies to ask
Topic, to realize material between each step it is abundant, efficiently mixing just need consumptive material and experimental facilities structurally and functionally
Carry out it is various consider, can avoid such as at high cost, poor sensitivity existing in the prior art, structure is complicated, operation and
The problems such as associated assay devices are excessively complicated.
Summary of the invention
Aiming at the shortcomings in the prior art, present invention solves the technical problem that being to provide a kind of material point deposits formula mixing dress
It sets, it is independent close in different material accommodating chambers by separately depositing in each component material needed for reaction solution or extracting solution
Envelope saves, and mixes each material when reaction, and it is cumbersome to avoid Extemporaneous reaction solution or extracting solution during the experiment
Operation, improves conventional efficient and the degree of automation.
Formula mixing arrangement is deposited present invention solves the technical problem that also residing in and providing a kind of material point, not only self structure letter
It is single and easy to operate, the complexity of the matched equipment used can be effectively reduced.
In order to solve the above-mentioned technical problem, it includes the first raw material that material provided in an embodiment of the present invention point, which deposits formula mixing arrangement,
Storehouse, the second raw material cabin connecting with first raw material cabin are equipped with first in the first raw material cabin in second raw material cabin
The first barrier layer that the second raw material in raw material and the second raw material cabin separates；Divide in first raw material cabin and the second raw material cabin
Not She You the first hollow cavity and the second hollow cavity, be provided in first hollow cavity and slid in the first hollow cavity
Thrust assemblies, when thrust assemblies are mobile to the direction of the second raw material cabin, thrust assemblies front end punctures first barrier layer, and
The first raw material in first hollow cavity is pressed in the second hollow cavity by the opening of the first barrier layer, the first raw material and
The second raw material mixing in two hollow cavities.
The top of the thrust assemblies is equipped with piston head, and the inner wall sealing of the piston head and first hollow cavity connects
Touching；The front end of the thrust assemblies is in syringe needle shape.
First hollow cavity has positioned at first cylindrical cavity on the first raw material cabin top and under the first raw material cabin
Second cylindrical cavity in portion, first cylindrical cavity and the second cylindrical cavity extend through the upper and lower part of first raw material cabin；
The length of first cylindrical cavity is greater than the length of the second cylindrical cavity, the diameter of the first cylindrical cavity less than the second cylindrical cavity diameter, and
And the annular slot extended to the first raw material cabin outer surface is equipped at the middle part of the second cylindrical cavity.
The interface channel being additionally provided in second raw material cabin above second hollow cavity, the interface channel
Through second raw material cabin top and communicated with second hollow cavity, the interface channel includes from top to bottom successively
The first conical cavity, third cylindrical cavity, the second conical cavity and the 4th cylindrical cavity being arranged, first barrier layer are located at the third
In cylindrical cavity and the wall surface of third cylindrical cavity is tightly connected.
The outer surface on second raw material cabin top is equipped with the annular protrusion being adapted with the annular slot, the ring
Shape protrusion buckles into the annular slot and the top of the second raw material cabin is fitted in second cylindrical cavity, and by described first
Raw material cabin links together with the second raw material cabin.The present invention includes the mixing by realizing material between adjacent feed storehouse,
Material preferably of the invention point deposits the first raw material cabin in formula mixing arrangement and the second raw material cabin can be individually by first
Raw material and the second raw material are stored in the first hollow cavity and the second hollow cavity, and by the first barrier layer by the first raw material and
Second raw material separates, in material mixing, by the way that the thrust assemblies slid in the first hollow cavity, thrust assemblies front end is arranged in
First barrier layer is punctured, and the first raw material in the first hollow cavity is pressed in second by the opening of the first barrier layer
In cavity body, to realize that the first raw material sufficiently, is efficiently mixed with the second raw material in the second hollow cavity.
As a preferred solution of the present invention, it is further that a kind of material point that the embodiment of the present invention provides deposits formula mixing arrangement
Including some or all of following technical characteristic:
Preferably, in one embodiment of the present invention, the depth of the first conical cavity is less than the depth of second conical cavity
Degree, the diameter of the 4th cylindrical cavity are less than the diameter of the third cylindrical cavity；Second raw material cabin have the first cylindrical wall,
Second cylindrical wall and third cylindrical wall, the annular protrusion are arranged on the outer surface of first cylindrical wall；First circle
The diameter of second cylindrical wall of post jamb is greater than the diameter of first cylindrical wall, and the outer surface of the third cylindrical wall is equipped with
External screw thread.
Further, in one embodiment of the present invention, the top of first cylindrical cavity is equipped with the gear to extend internally
Block, the block are uniformly distributed along the periphery of the first cylindrical cavity.
Preferably, in one embodiment of the present invention, the outer surface of second cylindrical wall is uniformly set along its periphery
It is equipped with arc-shaped protrusion.
Further, the thrust assemblies further include a mobile bar and support blade, and the support blade is located at the work
The top of chock plug, the mobile bar are located at the top of the support blade.
More preferably, the support blade of stating includes three blades, and blade is uniformly distributed along the central axis of thrust assemblies.
Preferably, first raw material is liquid material, and second raw material is liquid material or solid material.
Preferably, in another embodiment of the invention, it further includes third raw material cabin, institute that the material point, which deposits formula mixing arrangement,
It states third raw material cabin to be threadedly coupled with second raw material cabin, be equipped in the third raw material cabin by second in the second raw material cabin
The second barrier layer that third raw material in raw material and third raw material cabin separates, the third raw material cabin is interior to be equipped with third hollow cavity
Body；After the thrust assemblies puncture the first barrier layer, when continuing mobile to the direction of third raw material cabin, terminal spine before thrust assemblies
Second barrier layer is broken, and the mixture of the first raw material and the second raw material in the second hollow cavity is passed through into the second barrier layer
Opening press in third hollow cavity, the third raw material in the mixture of the first raw material and the second raw material and third hollow cavity
Mixing.
Preferably, the top of the third raw material cabin is run through in one end of the third hollow cavity, goes back in third raw material cabin
Equipped with the flow pass being located at below the third hollow cavity, the flow pass runs through the lower part of the third raw material cabin simultaneously
It is communicated with the third hollow cavity, the flow pass includes the third conical cavity set gradually from top to bottom, the 5th cylinder
Chamber, the 4th conical cavity and the 6th cylindrical cavity, second barrier layer be located at the top of the third hollow cavity and in third
The wall surface of cavity body is tightly connected, and the depth of third conical cavity is less than the depth of the 4th conical cavity, the 6th cylindrical cavity
Diameter be less than the diameter of the 5th cylindrical cavity, the other end of third hollow cavity is connected with flow pass.
Preferably, the length of first cylindrical cavity is greater than the length of second raw material cabin.
Preferably, material point deposits multiple originals in formula mixing arrangement comprising being mutually linked in other embodiments of the invention
It includes first, second and third that the material that feed bin, i.e. other embodiments of the invention provide, which point deposits formula mixing arrangement ... ..N raw material
The mixing of various materials is realized in storehouse by the mixing between two adjacent raw material cabins.
Material provided in an embodiment of the present invention point deposits formula mixing arrangement, has the following beneficial effects:
Material provided in an embodiment of the present invention point deposits formula mixing arrangement, by realizing material between adjacent feed storehouse
Mixing, material preferably of the invention point deposit the first raw material cabin in formula mixing arrangement and the second raw material cabin can individually by
First raw material and the second raw material are stored in the first hollow cavity and the second hollow cavity, and former by first by the first barrier layer
Material and the second raw material separate, in material mixing, by be arranged in the thrust assemblies slid in the first hollow cavity puncture it is described
First barrier layer, and the first raw material in the first hollow cavity is pressed into the second hollow cavity by the opening of the first barrier layer
In, to realize that the first raw material sufficiently, is efficiently mixed with the second raw material in the second hollow cavity, overcome nucleic acid, albumen etc.
In the extraction of substance, detection process between each step due to the linkings operation such as material mixing existing for trivial operations, sample or examination
The problem of agent vulnerable to pollution.In addition, material of the invention point deposits formula mixing arrangement, not only self structure is simple, but also operation side
Just, since the first raw material cabin and the second raw material cabin pass through the convex annular on the annular slot and the first cylindrical wall on the second cylindrical cavity
The leakproofness that ensure that between raw material cabin together is snapped connection between rising, it will be needed for reaction solution or extracting solution
Each component material separately deposits in independent sealed preservation in different material accommodating chambers, is needing to mix two different materials
When, first barrier layer is punctured by thrust assemblies front end and mixes the different materials in different material accommodating chamber, and
Sufficiently it can dissolve or mix and pushing thrust assemblies back and forth enables the raw material being located in different material storehouse disturbance, from
And interfering with each other before this mixed process for unclassified stores between neighboring chambers is effectively avoided, unnecessary pollution is caused, is influenced
The purity and quality for extracting final product, avoid Extemporaneous reaction solution during the experiment or extracting solution troublesome operation, mention
High conventional efficient and the degree of automation, further, the second raw material cabin and third raw material cabin of another embodiment of the present invention
It is together by a threaded connection, so that type of attachment between more raw material cabins is provided, to realize that more materials are blended in
Together, mixed liquor directly is generated by mixing manually to add without Extemporaneous, avoid existing during mixing
Such as at high cost present in technology, poor sensitivity, the problems such as structure is complicated, operation and associated assay devices are excessively complicated.
Detailed description of the invention
Fig. 1 is the structural schematic diagram that the material point that the preferred embodiment of the present invention provides deposits formula mixing arrangement.
Fig. 2 is the structural schematic diagram for the thrust assemblies that the material point that the preferred embodiment of the present invention provides deposits formula mixing arrangement.
Fig. 3 is the stereochemical structure that the material point that the preferred embodiment of the present invention provides deposits the first raw material cabin in formula mixing arrangement
Schematic diagram.
Fig. 4 is the cross-section structure that the material point that the preferred embodiment of the present invention provides deposits the first raw material cabin in formula mixing arrangement
Schematic diagram.
Fig. 5 is the stereochemical structure that the material point that the preferred embodiment of the present invention provides deposits the second raw material cabin in formula mixing arrangement
Schematic diagram.
Fig. 6 is the cross-section structure that the material point that the preferred embodiment of the present invention provides deposits the second raw material cabin in formula mixing arrangement
Schematic diagram.
Fig. 7 be another embodiment of the present invention provides material point deposit the structural schematic diagram of formula mixing arrangement.
Specific embodiment
The embodiment of the present invention provide a kind of material point deposit formula mixing arrangement include the first raw material cabin, with first raw material cabin
Second raw material cabin of connection, being equipped in second raw material cabin will be in the first raw material and the second raw material cabin in the first raw material cabin
The first barrier layer that second raw material separates；Be respectively equipped in first raw material cabin and the second raw material cabin the first hollow cavity and
Second hollow cavity is provided with the thrust assemblies slid in the first hollow cavity, thrust assemblies in first hollow cavity
When mobile to the direction of the second raw material cabin, thrust assemblies front end punctures first barrier layer, and will be in the first hollow cavity
First raw material is pressed in the second hollow cavity by the opening of the first barrier layer, second in the first raw material and the second hollow cavity
Raw material mixing；The top of the thrust assemblies is equipped with piston head, the inner wall sealing of the piston head and first hollow cavity
Contact；The front end of the thrust assemblies is in syringe needle shape；First hollow cavity has first positioned at the first raw material cabin top
Cylindrical cavity and the second cylindrical cavity positioned at the first raw material cabin lower part, first cylindrical cavity and the second cylindrical cavity extend through described
The upper and lower part of first raw material cabin；The length of first cylindrical cavity is greater than the length of the second cylindrical cavity, the diameter of the first cylindrical cavity
Less than the diameter of the second cylindrical cavity, and the annular snap extended to the first raw material cabin outer surface is equipped at the middle part of the second cylindrical cavity
Slot；The interface channel being additionally provided with above second hollow cavity in second raw material cabin, the interface channel run through
The top of second raw material cabin is simultaneously communicated with second hollow cavity, and the interface channel includes setting gradually from top to bottom
The first conical cavity, third cylindrical cavity, the second conical cavity and the 4th cylindrical cavity, first barrier layer is located at the third cylinder
In chamber and the wall surface of third cylindrical cavity is tightly connected；The outer surface on second raw material cabin top is equipped with and the annular slot
Adaptable annular protrusion, the annular protrusion buckle into the annular slot top of the second raw material cabin being fitted to described
In two cylindrical cavities, and first raw material cabin is linked together with the second raw material cabin.
Wherein, the depth of the first conical cavity is less than the depth of second conical cavity, and the diameter of the 4th cylindrical cavity is small
In the diameter of the third cylindrical cavity；Second raw material cabin has the first cylindrical wall, the second cylindrical wall and third cylindrical wall, institute
Annular protrusion is stated to be arranged on the outer surface of first cylindrical wall；The diameter of second cylindrical wall of first cylindrical wall
Greater than the diameter of first cylindrical wall, the outer surface of the third cylindrical wall is equipped with external screw thread.
Wherein, the top of first cylindrical cavity is equipped with the block to extend internally, circle of the block along the first cylindrical cavity
Circumferential surface is uniformly distributed.
Wherein, the outer surface of second cylindrical wall is evenly arranged with arc-shaped protrusion along its periphery.
Wherein, the thrust assemblies further include a mobile bar and support blade, and the support blade is located at the piston head
Top, the mobile bar is located at the top of the support blade.
Wherein, the support blade includes three blades, and blade is uniformly distributed along the central axis of thrust assemblies.
Wherein, first raw material is liquid material, and second raw material is liquid material or solid material.
Wherein, it further includes third raw material cabin that the material point, which deposits formula mixing arrangement, the third raw material cabin and second original
Feed bin is threadedly coupled, and is equipped in the third raw material cabin by the third in the second raw material and third raw material cabin in the second raw material cabin
The second barrier layer that raw material separates, the third raw material cabin is interior to be equipped with third hollow cavity；When the thrust assemblies puncture
After one barrier layer, when continuing mobile to the direction of third raw material cabin, thrust assemblies front end punctures second barrier layer, and by the
The mixture of the first raw material and the second raw material in two hollow cavities presses to third hollow cavity by the opening of the second barrier layer
In, the mixture of the first raw material and the second raw material is mixed with the third raw material in third hollow cavity.
Wherein, the top of the third raw material cabin is run through in one end of the third hollow cavity, also sets in third raw material cabin
Have be located at the third hollow cavity below flow pass, the flow pass through the third raw material cabin lower part and with
The third hollow cavity communicates, the flow pass include the third conical cavity set gradually from top to bottom, the 5th cylindrical cavity,
4th conical cavity and the 6th cylindrical cavity, second barrier layer be located at the top of the third hollow cavity and with third hollow cavity
The wall surface of body is tightly connected, and the depth of third conical cavity is less than the depth of the 4th conical cavity, the 6th cylindrical cavity it is straight
Diameter is less than the diameter of the 5th cylindrical cavity, and the other end of third hollow cavity is connected with flow pass.
Wherein, the length of first cylindrical cavity is greater than the length of second raw material cabin.
Wherein, material point deposits multiple raw materials in formula mixing arrangement comprising being mutually linked in other embodiments of the invention
It includes first, second and third that the material that storehouse, i.e. other embodiments of the invention provide, which point deposits formula mixing arrangement ... ..N raw material cabin,
The mixing of various materials is realized by the mixing between two adjacent raw material cabins.
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Shown in Fig. 1, the material point that the preferred embodiment of the present invention provides deposits the structural schematic diagram of formula mixing arrangement.The material point
Formula mixing arrangement is deposited applied in nucleic acid or albumen preparation and detection device, it is intended in nucleic acid or albumen preparation and detection process
Material mixing between adjacent step provide it is a kind of efficiently, efficiently solution, but be not limited thereto, in practical application
In, the material mixing can also be applied to the material mixing of other biological.
As shown in figures 1 to 6, the material that the preferred embodiment of the present invention provides point deposit formula mixing arrangement include the first raw material cabin 10,
The second raw material cabin 20 connecting with first raw material cabin 10, being equipped in second raw material cabin 20 will be in the first raw material cabin 10
The first barrier layer 30 that the second raw material in first raw material and the second raw material cabin 20 separates；First raw material cabin 10 and second
It is respectively equipped with the first hollow cavity 11 and the second hollow cavity 21 in raw material cabin 20, is provided in first hollow cavity 11
The thrust assemblies 40 slid in first hollow cavity 11, when thrust assemblies 40 are mobile to the direction of the second raw material cabin 20, pushing group
40 front end of part punctures first barrier layer 30, and the first raw material in the first hollow cavity 11 is passed through the first barrier layer 30
Opening presses in the second hollow cavity 21, and the first raw material is mixed with the second raw material in the second hollow cavity 21.Of the invention
Material point is deposited in formula mixing arrangement, and the first raw material is liquid material, and the second raw material is liquid material or solid material, the first raw material
Push liquid material to second raw material convenient for thrust assemblies 40 for liquid material, so that the first raw material and the mixing of the second raw material, real
Between existing nucleic acid or albumen preparation and each step of detection process material it is abundant, efficiently mix.
Specifically, as shown in Fig. 2, the top that material of the invention point deposits the thrust assemblies 40 of formula mixing arrangement is equipped with piston
First 41, piston head 41 is plastic part, and in sealing contact with the inner wall of first hollow cavity 11, prevents thrust assemblies 40 the
During sliding in one hollow cavity 11, the first raw material in the first hollow cavity 11 is from piston head 41 and the first hollow cavity
Gap outflow between 11.The front end of the thrust assemblies 40 is in syringe needle shape, convenient for the first hollow cavity 11 and second is hollow
The first barrier layer 30 between cavity 21 punctures.Further, the thrust assemblies 40 further include a mobile bar 42 and support
Blade 43, the support blade 43 are located at the top of the piston head 41, and the mobile bar 42 is located at the support blade 43
Top, mobile bar 42 is T-shaped, convenient for applying pressure to thrust assemblies 40, and rotates to thrust assemblies 40, the support leaf
Piece 43 includes three blades, and blade is uniformly distributed along the central axis of thrust assemblies 40, and support blade 43 can effectively support fixed
Lever 42 and entire thrust assemblies 40 are displaced, entire thrust assemblies 40 is made to be unlikely to direction deviation occur in pushing traveling process
Or the case where distorting.
In addition, as shown in Figure 3 and Figure 4, the first hollow cavity 11 of the invention has positioned at 10 top of the first raw material cabin
First cylindrical cavity 12 and the second cylindrical cavity 13 positioned at 10 lower part of the first raw material cabin, first cylindrical cavity 12 and the second cylindrical cavity
13 extend through the upper and lower part of first raw material cabin 10；The length of first cylindrical cavity 12 is greater than the length of the second cylindrical cavity 13
Degree, the diameter of the first cylindrical cavity 12 and are equipped at the middle part of the second cylindrical cavity 13 to the less than the diameter of the second cylindrical cavity 13
The annular slot 50 that one raw material cabin outer surface extends.Preferably, the top of first cylindrical cavity 12 is equipped with the gear to extend internally
Block 121, the block 121 are uniformly distributed along the periphery of the first cylindrical cavity 12, and block 121 mainly prevents thrust assemblies 40
Piston head 41 skids off the first cylindrical cavity 12.
As shown in Figure 5 and Figure 6, it is additionally provided with above second hollow cavity 21 in the second raw material cabin 20 of the invention
Interface channel 22, the interface channel 22 through second raw material cabin 20 top and with 21 phase of the second hollow cavity
Logical, the interface channel 22 includes the first conical cavity 221, third cylindrical cavity 222, the second conical cavity set gradually from top to bottom
223 and the 4th cylindrical cavity 224, first barrier layer 30 is located in the third cylindrical cavity 222 and the wall of third cylindrical cavity 222
Face is tightly connected, it is preferable that first barrier layer 30 is film or other can play sealing function component.Further,
The depth of first conical cavity 221 is less than the depth of second conical cavity 223, and the diameter of the 4th cylindrical cavity 224 is less than institute
The diameter of third cylindrical cavity 222 is stated, such setting passes through interface channel 22 convenient for the first raw material and is flowed into the second raw material cabin
The second raw material mixing in 20 the second hollow cavity 21, rapidly and in the second hollow cavity 21.
Further, the outer surface on 20 top of the second raw material cabin is equipped with the ring being adapted with the annular slot 50
Shape protrusion 60, the annular protrusion 60 buckle into the annular slot 50 top of second raw material cabin 20 being fitted to described second
In cylindrical cavity 21, and first raw material cabin is linked together with the second raw material cabin.More specifically, second raw material cabin
20 have the first cylindrical wall 23, the second cylindrical wall 24 and third cylindrical wall 25, and the outer surface of first cylindrical wall 23 is equipped with
The annular protrusion 60 being adapted with the annular slot 50, the annular protrusion 60 buckle into the first circle in the annular slot 50
Post jamb 23 is fitted in second cylindrical cavity 13, and first raw material cabin 10 and the second raw material cabin 20 are linked together.
The diameter of second cylindrical wall 24 is greater than the diameter of first cylindrical wall 23, and the outer surface of the third cylindrical wall 25 is equipped with
External screw thread, it is preferable that the outer surface of second cylindrical wall 24 is evenly arranged with arc-shaped protrusion 241 along its periphery, is convenient for
The second raw material cabin 20 is clamped using fixture and makes its rotation, and will be of the invention by the external screw thread of 25 outer surface of third cylindrical wall
Material point is deposited formula mixing arrangement and is mounted in other devices, so that the mixture of the first raw material and the second raw material of the invention imports
Into other devices.
In another embodiment of the invention, as shown in fig. 7, it further includes third raw material that the material point, which deposits formula mixing arrangement,
Storehouse 70, the third raw material cabin 70 are threadedly coupled with second raw material cabin 20, are equipped in the third raw material cabin 70 by second
The second barrier layer 80 that the third raw material in the second raw material and third raw material cabin in raw material cabin 20 separates, the third raw material
Third hollow cavity 71 is equipped in storehouse 70, the inner wall on the top of third hollow cavity 71 is equipped with and 25 appearance of third cylindrical wall
The internal screw thread that the external screw thread in face is screwed on, by the threaded connection of external screw thread and internal screw thread by third raw material cabin 70 and the second raw material cabin
20 connect.The flow pass 90 positioned at 71 lower section of third hollow cavity, the stream are additionally provided in third raw material cabin 70
Channel 90 is run through the lower part of the third raw material cabin 70 and is communicated with the third hollow cavity 71 out, and the flow pass 90 wraps
The third conical cavity 91 set gradually from top to bottom, the 5th cylindrical cavity 92, the 4th conical cavity 93 and the 6th cylindrical cavity 94 are included, it is described
Second barrier layer 80 is located at the top of the third hollow cavity 71 and is tightly connected with the wall surface of third hollow cavity 71, into one
Step ground, the depth of third conical cavity 91 are less than the depth of the 4th conical cavity 93, and the diameter of the 6th cylindrical cavity 94 is less than
The diameter of 5th cylindrical cavity 92 is arranged such and passes through flow pass outflow convenient for mixed material.
When specific implementation, after the thrust assemblies 40 puncture the first barrier layer 30, continue the side to third raw material cabin 70
To it is mobile when, 40 front end of thrust assemblies punctures second barrier layer 80, and by the second hollow cavity 21 the first raw material and
The mixture of second raw material is pressed in third hollow cavity 71 by the opening of the second barrier layer 80, the first raw material and the second raw material
Mixture mixed with the third raw material in third hollow cavity 71, specifically, one end of the third hollow cavity 71 is run through
The other end on the top of the third raw material cabin 70, third hollow cavity 71 is connected with flow pass 90, when the first raw material,
After two raw materials and the mixing of third raw material, exported by the flow pass 90 of third raw material cabin 70.In order to guarantee 40 energy of thrust assemblies
The second barrier layer 80 is enough punctured, the length of first cylindrical cavity 12 is greater than the length of second raw material cabin 20, can protect in this way
The front end for demonstrate,proving thrust assemblies 40 can pass through the second raw material cabin 20, then contact the second barrier layer 80 in third raw material cabin 70 and incite somebody to action
It is punctured.
In addition, from the point of view of the structure setting of the first raw material cabin 10 and the joining place of the second raw material cabin 20, in order to sufficiently,
Efficiently, hermetically material is mixed, sealing ring is set in the first cylindrical wall 23 and the second cylindrical cavity 13, further prevents first
The first raw material 10 in raw material cabin 10 is flowed out by the gap between the first cylindrical wall 23 and the second cylindrical cavity 13.It is corresponding to it
Ground is also provided with sealing ring in the joining place of the second raw material cabin 20 and third raw material cabin 70, prevents the outflow of material.
It should be noted that structure can be seen that material provided in an embodiment of the present invention and point deposit formula mixing from Fig. 1 and 7
Device is or to include the first raw material cabin 10, the second raw material cabin 20 and the comprising the first raw material cabin 10 and the second raw material cabin 20
Three raw material cabins 70, in fact, only being mixed using the material of two raw material cabins as the present invention program's in the preferred embodiment of the present invention
A kind of preferred embodiment is to illustrate that the present invention is nucleic acid or albumen preparation and detection process to start with from simple structure
In one kind for providing of material mixing between adjacent step efficiently, efficiently solution, but be not limited thereto, actually answering
In, the material due to being related to multiple steps in nucleic acid extraction and detection process is mixed, in other realities of the invention
Applying material point in example and depositing includes the multiple raw material cabins being mutually linked in formula mixing arrangement.That is the object of other embodiments of the invention offer
It includes first, second and third that material, which point deposits formula mixing arrangement ... ..N raw material cabin, passes through mixing between two adjacent raw material cabins
The mixing to realize various materials is closed, material is abundant, efficient between realization nucleic acid or albumen preparation and each step of detection process
Ground mixing, avoids that such as at high cost, poor sensitivity existing in the prior art, structure is complicated, operation and associated assay devices mistake
In the complexity the problems such as.
The material point that above-described embodiment discloses deposits formula mixing arrangement, by realizing the mixed of material between adjacent feed storehouse
It closes, material preferably of the invention point deposits the first raw material cabin 10 in formula mixing arrangement and the second raw material cabin 20 can be individually
First raw material and the second raw material are stored in the first hollow cavity 11 and the second hollow cavity 21, and pass through the first barrier layer 30
First raw material and the second raw material are separated, in material mixing, by the way that the pushing group slid in the first hollow cavity 11 is arranged in
Part 40 punctures first barrier layer 30, and the first raw material in the first hollow cavity 11 is passed through to the opening of the first barrier layer 30
It presses in the second hollow cavity 21, to realize that the second raw material in the first raw material and the second hollow cavity 21 is abundant, efficient
Mixing, overcome the extractions of the substances such as nucleic acid, albumen, in detection process between each step because the linkings operation due to such as material mixing exists
Trivial operations, sample or the problem of reagent vulnerable to pollution.In addition, material of the invention point deposits formula mixing arrangement, not only itself
Structure is simple, and easy to operate, since the first raw material cabin 10 and the second raw material cabin 20 pass through the annular on the second cylindrical cavity 13
The sealing that ensure that between raw material cabin together is snapped connection between annular protrusion 60 on card slot 50 and the first cylindrical wall 23
Property, each component material needed for reaction solution or extracting solution is separately deposited in independent sealed in different material accommodating chambers
It saves, when needing to mix two different materials, puncturing first barrier layer 30 by 40 front end of thrust assemblies will not
With the different material mixing in material accommodating chamber, and can be by pushing thrust assemblies to make to be located in different material storehouse back and forth
Raw material disturbance and be able to sufficiently dissolve or mix, to effectively avoid mixing herein for unclassified stores between neighboring chambers
Cheng Qian interferes with each other, and causes unnecessary pollution, influences the purity and quality of extracting final product, avoids and face during the experiment
When prepare reaction solution or extracting solution troublesome operation, improve conventional efficient and the degree of automation.Further, of the invention another
The second raw material cabin 20 and third raw material cabin 70 of one embodiment are together by a threaded connection, to provide more raw material cabins
Between type of attachment, to realize that more materials mix, directly by mixing generate mixed liquor without temporarily matching
The artificial addition of system, avoid during mixing such as at high cost, poor sensitivity existing in the prior art, structure is complicated,
The problems such as operation and excessively complicated associated assay devices.
The above is a preferred embodiment of the present invention, it is noted that for those skilled in the art
For, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also considered as
Protection scope of the present invention.
Claims (10)
1. a kind of material point deposits formula mixing arrangement, it is characterised in that: connect including the first raw material cabin, with first raw material cabin
Second raw material cabin is equipped in second raw material cabin second in the first raw material and the second raw material cabin in the first raw material cabin is former
Expect the first barrier layer separated；It is respectively equipped in the first hollow cavity and second in first raw material cabin and the second raw material cabin
Cavity body, is provided with the thrust assemblies slid in the first hollow cavity in first hollow cavity, and thrust assemblies are to second
When the direction of raw material cabin is mobile, thrust assemblies front end punctures first barrier layer, and first in the first hollow cavity is former
Material is pressed in the second hollow cavity by the opening of the first barrier layer, and the second raw material in the first raw material and the second hollow cavity is mixed
It closes；
The top of the thrust assemblies is equipped with piston head, and the inner wall of the piston head and first hollow cavity is in sealing contact；
The front end of the thrust assemblies is in syringe needle shape；
First hollow cavity has positioned at first cylindrical cavity on the first raw material cabin top and positioned at the first raw material cabin lower part
Second cylindrical cavity, first cylindrical cavity and the second cylindrical cavity extend through the upper and lower part of first raw material cabin；First
The length of cylindrical cavity is greater than the length of the second cylindrical cavity, the diameter of the first cylindrical cavity less than the second cylindrical cavity diameter, and
The middle part of second cylindrical cavity is equipped with the annular slot extended to the first raw material cabin outer surface；
The interface channel being additionally provided with above second hollow cavity in second raw material cabin, the interface channel run through
The top of second raw material cabin is simultaneously communicated with second hollow cavity, and the interface channel includes setting gradually from top to bottom
The first conical cavity, third cylindrical cavity, the second conical cavity and the 4th cylindrical cavity, first barrier layer is located at the third cylinder
In chamber and the wall surface of third cylindrical cavity is tightly connected；
The outer surface on second raw material cabin top is equipped with the annular protrusion being adapted with the annular slot, the convex annular
It rises and buckles into the annular slot top of the second raw material cabin is fitted in second cylindrical cavity, and by first raw material
Storehouse links together with the second raw material cabin.
2. material as described in claim 1 point deposits formula mixing arrangement, it is characterised in that: the depth of the first conical cavity is less than described
The depth of second conical cavity, the diameter of the 4th cylindrical cavity are less than the diameter of the third cylindrical cavity；Second raw material cabin
With the first cylindrical wall, the second cylindrical wall and third cylindrical wall, the appearance of first cylindrical wall is arranged in the annular protrusion
On face；The diameter of second cylindrical wall is greater than the diameter of first cylindrical wall, and the outer surface of the third cylindrical wall is equipped with
External screw thread.
3. material as described in claim 1 point deposits formula mixing arrangement, it is characterised in that: the top of first cylindrical cavity is equipped with
The block to extend internally, the block are uniformly distributed along the periphery of the first cylindrical cavity.
4. material as described in claim 1 point deposits formula mixing arrangement, it is characterised in that: the outer surface edge of second cylindrical wall
Its periphery is evenly arranged with arc-shaped protrusion.
5. material as described in claim 1 point deposits formula mixing arrangement, it is characterised in that: the thrust assemblies further include one mobile
Bar and support blade, the support blade are located at the top of the piston head, and the mobile bar is located at the upper of the support blade
Side.
6. material as claimed in claim 5 point deposits formula mixing arrangement, it is characterised in that: the support blade includes three leaves
Piece, blade are uniformly distributed along the central axis of thrust assemblies.
7. material as described in claim 1 point deposits formula mixing arrangement, it is characterised in that: first raw material is liquid material,
Second raw material is liquid material or solid material.
8. material as claimed in claim 2 point deposits formula mixing arrangement, it is characterised in that: the material point is deposited formula mixing arrangement and also wrapped
Third raw material cabin is included, the third raw material cabin is threadedly coupled with second raw material cabin, is equipped in the third raw material cabin by the
The second barrier layer that the third raw material in the second raw material and third raw material cabin in two raw material cabins separates, the third raw material cabin
It is interior to be equipped with third hollow cavity；After the thrust assemblies puncture the first barrier layer, continue mobile to the direction of third raw material cabin
When, thrust assemblies front end punctures second barrier layer, and by the second hollow cavity the first raw material and the second raw material it is mixed
Zoarium is pressed in third hollow cavity by the opening of the second barrier layer, in the mixture and third of the first raw material and the second raw material
Third raw material mixing in cavity body.
9. material as claimed in claim 8 point deposits formula mixing arrangement, it is characterised in that: one end of the third hollow cavity is passed through
Wear the top of the third raw material cabin, the flow pass being additionally provided in third raw material cabin below the third hollow cavity,
The flow pass runs through the lower part of the third raw material cabin and communicates with the third hollow cavity, and the flow pass includes
Third conical cavity, the 5th cylindrical cavity, the 4th conical cavity and the 6th cylindrical cavity set gradually from top to bottom, second barrier layer
Positioned at the third hollow cavity top and be tightly connected with the wall surface of third hollow cavity, the depth of third conical cavity is less than
The depth of 4th conical cavity, the diameter of the 6th cylindrical cavity are less than the diameter of the 5th cylindrical cavity, third hollow cavity
The other end of body is connected with flow pass；The length of first cylindrical cavity is greater than the length of second raw material cabin.
10. material as described in claim 1 point deposits formula mixing arrangement, it is characterised in that: the material point is deposited in formula mixing arrangement
Include the multiple raw material cabins being mutually linked.
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| CN107089441B (en) * | 2017-06-13 | 2020-06-02 | 苏州中科生物医用材料有限公司 | Cartilage repair instrument |
| CN110117143B (en) * | 2019-05-10 | 2021-09-17 | 大成科创基础建设股份有限公司 | Method for curing slurry by using magnesium-based composite material |
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| EP0927077A4 (en) * | 1996-09-18 | 2000-07-19 | Promega Corp | Improved filtration system and method |
| WO2006023471A2 (en) * | 2004-08-18 | 2006-03-02 | Preanalytix Gmbh | Additive, method, and article for dna collection, stabilization, and purification |
| US8584838B2 (en) * | 2009-09-08 | 2013-11-19 | Sdi (North America), Inc. | Container for mixing of components |
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| CN1501080A (en) * | 2002-11-14 | 2004-06-02 | - | Method, system and reaction vessel for processing a biological sample contained in a liquid |
| CN101321586A (en) * | 2005-12-12 | 2008-12-10 | 西泰克公司 | Method and apparatus for obtaining aliquot from liquid-based cytological sample |
| CN102639689A (en) * | 2009-09-29 | 2012-08-15 | 盖克基金会 | Portable enrichment, aliquoting, and testing device of microorganisms and toxins |
| CN203941020U (en) * | 2014-07-14 | 2014-11-12 | 冯宝仁 | A kind of food inspection different depth sampler |
| CN204602089U (en) * | 2015-04-30 | 2015-09-02 | 余家昌 | A kind of material divides deposits formula mixing arrangement |
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Effective date of registration: 20170519 Address after: 200120 Shanghai Pudong New Area city wide 222 Lane 6, 5 Dan Applicant after: Bio Technology (Shanghai) Co., Ltd. Address before: 361, Ruth Town, San Francisco, Calif. Applicant before: Yu Jiachang Applicant before: Ai Jiesi bio tech ltd Applicant before: Bio Technology (Shanghai) Co., Ltd. |
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